B F Mitchell

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. doi request reprint Are animal models relevant to key aspects of human parturition?
    Bryan F Mitchell
    Department of Obstetrics and Gynecology, Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Regul Integr Comp Physiol 297:R525-45. 2009
  2. pmc Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes
    Hector N Aguilar
    Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 6:e20903. 2011
  3. pmc Quantification of rapid Myosin regulatory light chain phosphorylation using high-throughput in-cell Western assays: comparison to Western immunoblots
    Hector N Aguilar
    Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 5:e9965. 2010
  4. pmc Development and validation of primary human myometrial cell culture models to study pregnancy and labour
    Andrea A Mosher
    Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada
    BMC Pregnancy Childbirth 13:S7. 2013
  5. ncbi request reprint Intraperitoneal infusion of proinflammatory cytokines does not cause activation of the rat uterus during late gestation
    Bryan F Mitchell
    Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Am J Physiol Endocrinol Metab 289:E658-64. 2005
  6. ncbi request reprint Metabolites of progesterone and the pregnane X receptor: a novel pathway regulating uterine contractility in pregnancy?
    Bryan F Mitchell
    Department of Obstetrics and Gynecology, Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Obstet Gynecol 192:1304-13; discussion 1313-5. 2005
  7. ncbi request reprint Oxytocin and its receptor in the process of parturition
    B F Mitchell
    Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada
    J Soc Gynecol Investig 8:122-33. 2001
  8. ncbi request reprint Role of carboxy-extended forms of oxytocin in the rat uterus in the process of parturition
    B F Mitchell
    a Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada T5H 3V9
    Biol Reprod 59:1321-7. 1998
  9. ncbi request reprint Metabolism of oxytocin in rat uterus and placenta in late gestation
    B F Mitchell
    Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Biol Reprod 57:807-12. 1997
  10. ncbi request reprint Transcriptional regulation of oxytocin receptor by interleukin-1beta and interleukin-6
    B Schmid
    Perinatal Research Center, Department of Obstetrics and Gynecology, HMRC 220, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Endocrinology 142:1380-5. 2001

Collaborators

Detail Information

Publications33

  1. doi request reprint Are animal models relevant to key aspects of human parturition?
    Bryan F Mitchell
    Department of Obstetrics and Gynecology, Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Regul Integr Comp Physiol 297:R525-45. 2009
    ..We conclude that the application of novel concepts and new models are required to advance translational research in parturition...
  2. pmc Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes
    Hector N Aguilar
    Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 6:e20903. 2011
    ..This method is exemplified by quantifying the phosphorylation of myosin regulatory light chain (RLC) in cultured human uterine myocytes...
  3. pmc Quantification of rapid Myosin regulatory light chain phosphorylation using high-throughput in-cell Western assays: comparison to Western immunoblots
    Hector N Aguilar
    Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 5:e9965. 2010
    ....
  4. pmc Development and validation of primary human myometrial cell culture models to study pregnancy and labour
    Andrea A Mosher
    Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada
    BMC Pregnancy Childbirth 13:S7. 2013
    ..Labour has been described as an inflammatory process; therefore, the ability of these cells to respond to an inflammatory stimulus was also investigated...
  5. ncbi request reprint Intraperitoneal infusion of proinflammatory cytokines does not cause activation of the rat uterus during late gestation
    Bryan F Mitchell
    Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Am J Physiol Endocrinol Metab 289:E658-64. 2005
    ..The role of proinflammatory cytokines in the mechanism of parturition remains unclear...
  6. ncbi request reprint Metabolites of progesterone and the pregnane X receptor: a novel pathway regulating uterine contractility in pregnancy?
    Bryan F Mitchell
    Department of Obstetrics and Gynecology, Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Obstet Gynecol 192:1304-13; discussion 1313-5. 2005
    ..The purpose of this study was to determine the role of 5beta-dihydroprogesterone (5beta-DHP), acting through the nuclear receptor pregnane X receptor (PXR), in regulating uterine contractility...
  7. ncbi request reprint Oxytocin and its receptor in the process of parturition
    B F Mitchell
    Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada
    J Soc Gynecol Investig 8:122-33. 2001
    ..To review the potential roles of oxytocin (OT) and its receptor (OTR) in the regulation of parturition...
  8. ncbi request reprint Role of carboxy-extended forms of oxytocin in the rat uterus in the process of parturition
    B F Mitchell
    a Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada T5H 3V9
    Biol Reprod 59:1321-7. 1998
    ..However, these extended forms of OT have little direct biological activity and do not compete with OT for receptor binding. Their role in the process of parturition may be confined to acting as substrates for OT synthesis...
  9. ncbi request reprint Metabolism of oxytocin in rat uterus and placenta in late gestation
    B F Mitchell
    Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Biol Reprod 57:807-12. 1997
    ..It is doubtful that changes in these activities are major factors in regulating the increase in OT concentrations measured in rat intrauterine tissues at the time of parturition...
  10. ncbi request reprint Transcriptional regulation of oxytocin receptor by interleukin-1beta and interleukin-6
    B Schmid
    Perinatal Research Center, Department of Obstetrics and Gynecology, HMRC 220, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Endocrinology 142:1380-5. 2001
    ..Our findings suggest a role for IL-1beta and IL-6 in the transcriptional regulation of the human OT receptor gene...
  11. ncbi request reprint Effects of LPS and IL-6 on oxytocin receptor in non-pregnant and pregnant rat uterus
    X Fang
    Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Am J Reprod Immunol 44:65-72. 2000
    ..Little is known regarding the regulation of the timing of parturition. Recent evidence suggests an interaction between the immune system and uterine contractility in late gestation...
  12. ncbi request reprint Oxytocin does not directly affect vascular tone in vessels from nonpregnant and pregnant rats
    M E Miller
    Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Am J Physiol Heart Circ Physiol 282:H1223-8. 2002
    ..Although it may have an indirect effect in regulation of peripheral resistance, we conclude that OT is unlikely to play a direct role in the physiological regulation of vascular tone...
  13. ncbi request reprint Phorbol ester treatment of human myometrial cells suppresses expression of oxytocin receptor through a mechanism that does not involve activator protein-1
    Allison Ball
    Department of Obstetrics and Gynecology, Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Endocrinol Metab 291:E922-8. 2006
    ..We conclude that phorbol ester treatment decreases OTR expression, likely through a mechanism that does not involve AP-1...
  14. ncbi request reprint Myometrial activation and preterm labour: evidence supporting a role for the prostaglandin F receptor--a review
    D M Olson
    The Perinatal Research Centre, The CIHR Group in Perinatal Health and Disease, Department of Obstetrics and Gynaecology, The University of Alberta, Edmonton, Canada
    Placenta 24:S47-54. 2003
    ..Finally new data showing that administration of a specific FP antagonist delays preterm birth in sheep will be presented...
  15. ncbi request reprint Proinflammatory cytokines inhibit human placental 11beta-hydroxysteroid dehydrogenase type 2 activity through Ca2+ and cAMP pathways
    Iren Kossintseva
    Department of Obstetrics and Gynecology, Univ of Alberta, Edmonton, Canada
    Am J Physiol Endocrinol Metab 290:E282-8. 2006
    ..This could result in excessive fetal exposure to maternal cortisol. This mechanism might mediate part of the increased risk of metabolic and cardiovascular disease in adult offspring...
  16. ncbi request reprint Resistance exercise decreases the need for insulin in overweight women with gestational diabetes mellitus
    Gabrielle N Brankston
    Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada
    Am J Obstet Gynecol 190:188-93. 2004
    ..This study examines the effects of circuit-type resistance training on the need for insulin in women with gestational diabetes mellitus...
  17. doi request reprint Inflammatory processes in preterm and term parturition
    Inge Christiaens
    Perinatal Research Centre, Departments of Obstetrics and Gynecology, Pediatrics, Physiology, and Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada
    J Reprod Immunol 79:50-7. 2008
    ..In turn, cortisol may drive PG synthesis. Together these feed-forward mechanisms activate the uterus, trigger the production of uterine contractile stimulants and lead to labour and delivery...
  18. ncbi request reprint The interleukin 1beta-induced expression of human prostaglandin F2alpha receptor messenger RNA in human myometrial-derived ULTR cells requires the transcription factor, NFkappaB
    Dean B Zaragoza
    Department of Obstetrics and Gynecology, University of Alberta, Canada
    Biol Reprod 75:697-704. 2006
    ....
  19. ncbi request reprint Emerging tocolytics: challenges in designing and testing drugs to delay preterm delivery and prolong pregnancy
    David M Olson
    University of Alberta, AHFMR Interdisciplinary Team in Preterm Birth and Healthy Outcomes, Department of Obstetrics and Gynecology, 220 HMRC, Edmonton, T6G 2S2, Alberta, Canada
    Expert Opin Emerg Drugs 13:695-707. 2008
    ....
  20. doi request reprint Physiological pathways and molecular mechanisms regulating uterine contractility
    Hector N Aguilar
    Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    Hum Reprod Update 16:725-44. 2010
    ..Abnormal contractility might underlie common and important disorders such as infertility, implantation failure, dysmenorrhea, endometriosis, spontaneous miscarriage or preterm birth...
  21. ncbi request reprint Messenger RNA for progesterone receptor isoforms in the late-gestation rat uterus
    X Fang
    Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Am J Physiol Endocrinol Metab 283:E1167-72. 2002
    ..We conclude that there are significant changes in PR isoforms in late-gestation rat uterus. These changes may be regulated by estrogen and P4 and may influence the timing of parturition...
  22. ncbi request reprint Changes in 17 beta,20 alpha-hydroxysteroid dehydrogenase activity supporting an increase in the estrogen/progesterone ratio of human fetal membranes at parturition
    B F Mitchell
    Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Am J Obstet Gynecol 168:1377-85. 1993
    ..CONCLUSION: The enzyme 17 beta,20 alpha-hydroxysteroid dehydrogenase may be an important regulator of the local estrogen/progesterone ratio in fetal membranes around the time of parturition...
  23. ncbi request reprint Prostaglandin F2alpha and its receptor as activators of human decidua
    Shintaro Makino
    Perinatal Research Centre, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Semin Reprod Med 25:60-8. 2007
    ..These collective actions prepare the decidua for its role in parturition...
  24. pmc Relationship between gene expression and function of uterotonic systems in the rat during gestation, uterine activation and both term and preterm labour
    Patrice Arthur
    Perinatal Research Centre, Department of Obstetrics and Gynaecology, 220 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    J Physiol 586:6063-76. 2008
    ..This novel combination of functional and genetic expression analyses provides new insight into the physiology of parturition...
  25. ncbi request reprint Relative importance of maternal constitutional factors and glucose intolerance of pregnancy in the development of newborn macrosomia
    N Okun
    Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    J Matern Fetal Med 6:285-90. 1997
    ..Treatment of mothers with GDM may be masking the effect of more pronounced carbohydrate intolerance...
  26. ncbi request reprint Regulation of vascular tone during pregnancy: a novel role for the pregnane X receptor
    Kathryn A Hagedorn
    Perinatal Research Centre, Department of Obstetrics Gynecology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Hypertension 49:328-33. 2007
    ....
  27. ncbi request reprint Oxytocin and parturition: a role for increased myometrial calcium and calcium sensitization?
    Patrice Arthur
    Perinatal Research Center, Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Canada
    Front Biosci 12:619-33. 2007
    ..This, in turn, is necessary to facilitate the development of novel and effective strategies to reduce the incidence of preterm birth...
  28. ncbi request reprint Prostaglandin endoperoxide H synthase inhibitors and other tocolytics in preterm labour
    Bryan F Mitchell
    Department of Obstetrics and Gynaecology, Perinatal Research Centre, CIHR Group in Perinatal Health and Disease, University of Alberta, 220 HMRC, Edmonton, Alberta, Canada T6G2S2
    Prostaglandins Leukot Essent Fatty Acids 70:167-87. 2004
    ..Mixed inhibitors and isoform-specific inhibitors of PGHS have been developed, and their roles in delaying preterm labour are examined and compared to other tocolytics...
  29. ncbi request reprint In-home nursing care for women with high-risk pregnancies: outcomes and cost
    M J Harrison
    Faculty of Nursing, University of Alberta, Edmonton, Alberta, Canada
    Obstet Gynecol 97:982-7. 2001
    ....
  30. ncbi request reprint Synthesis of oxytocin analogues with replacement of sulfur by carbon gives potent antagonists with increased stability
    Jake L Stymiest
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    J Org Chem 70:7799-809. 2005
    ..These results suggest that peptides 8 and 18 and analogues thereof may be important leads into the development of a long-lasting, commercially available therapeutic for initiation of parturition and treatment of preterm labor...
  31. ncbi request reprint Synthesis of biologically active dicarba analogues of the peptide hormone oxytocin using ring-closing metathesis
    Jake L Stymiest
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2G2
    Org Lett 5:47-9. 2003
    ..Biological testing on rat uterus strips shows that cis compound 3 has an EC50 value of 38 ng/mL (EC50 for oxytocin is 2.7 ng/mL) whereas 5 and trans olefin 4 are less active. [reaction--see text]..
  32. pmc Performance profile of an outcome measure: morbidity assessment index for newborns
    Anila Verma
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada
    J Epidemiol Community Health 59:420-6. 2005
    ..This score was designed as a discriminative index of morbidity for the entire population of babies delivered >28 weeks gestation without a major congenital anomaly...