Genomes and Genes
Affiliation: University of Manitoba
- Loss of HLTF function promotes intestinal carcinogenesisSumit Sandhu
Department of Biochemistry and Medical Genetics, University of Manitoba, 745 Bannatyne Avenue, Winnipeg MB R3E 0J9, Canada
Mol Cancer 11:18. 2012..HLTF has also been found to be frequently inactivated by promoter hypermethylation in human colon cancers. Whether this epigenetic event is required for intestinal carcinogenesis is unknown...
- 3D nuclear organization of telomeres in the Hodgkin cell lines U-HO1 and U-HO1-PTPN1: PTPN1 expression prevents the formation of very short telomeres including "t-stumps"Hans Knecht
CHUS, Universite de Sherbrooke, Quebec, Canada
BMC Cell Biol 11:99. 2010..In vitro and in vivo formation of RS-cells is associated with the increase of very short telomeres including "t-stumps", telomere loss, telomeric aggregate formation and the generation of "ghost nuclei"...
- Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cellsKhajornsak Tragoolpua
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand
BMC Biotechnol 8:5. 2008....
- The three-dimensional organization of telomeres in the nucleus of mammalian cellsTony Chih Yuan Chuang
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, R3E 0V9, Canada
BMC Biol 2:12. 2004..It is, therefore, essential to analyze the 3D genome organization of the interphase nucleus in both normal and cancer cells...
- Initiation of telomere-mediated chromosomal rearrangements in cancerSabine Mai
Manitoba Institute of Cell Biology, CancerCare Manitoba, Department of Physiology, University of Manitoba, Winnipeg, Canada R3E 0V9
J Cell Biochem 109:1095-102. 2010..In this article, I discuss current data and concepts on telomere-mediated chromosomal rearrangements in cancer...
- Genomic instability in MycER-activated Rat1A-MycER cellsS Mai
Manitoba Institute of Cell Biology, Winnipeg, Canada
Chromosome Res 4:365-71. 1996..The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements...
- The significance of telomeric aggregates in the interphase nuclei of tumor cellsSabine Mai
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
J Cell Biochem 97:904-15. 2006..10: 3317-3326). In the present overview, we will discuss the role of telomeres in genome stability, recent findings on three-dimensional (3D) changes of telomeres in tumor interphase nuclei, and outline future avenues of research...
- Oncogenic remodeling of the three-dimensional organization of the interphase nucleus: c-Myc induces telomeric aggregates whose formation precedes chromosomal rearrangementsSabine Mai
Manitoba Institute of Cell Biology, University of Manitoba, CancerCare Manitoba, Winnipeg, Manitoba, Canada
Cell Cycle 4:1327-31. 2005..It induces the formation of telomeric aggregates and fusions that are followed by breakage-bridge fusion cycles, and lead to the onset of chromosomal rearrangements that are typical of tumor cells...
- c-Myc-induced genomic instabilitySabine Mai
Manitoba Institute of Cell Biology, Cancer Care Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada
J Environ Pathol Toxicol Oncol 22:179-99. 2003..Myc thus acts as a structural modifier of the genome and as a promoter of neoplastic transformation...
- The impact of p53 loss on murine plasmacytoma developmentSabine Mai
Manitoba Institute of Cell Biology, The Genomic Centre for Cancer Research and Diagnosis, Department of Physiology, The University of Manitoba, Winnipeg, Canada
Chromosome Res 10:239-51. 2002..The 42.2% incidence in N3p53-/- mice is similar to the percentages recorded in groups of conventional BALB/cAn mice. The possible mechanisms underlying the accelerated tumorigenesis and the shift in translocation patterns are discussed...
- Isolation of extrachromosomal elements by histone immunoprecipitationT I Kuschak
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, MB, Canada
Biotechniques 30:1064-8, 1070-2. 2001..We demonstrate the enrichment of immunoprecipitated DHFR-containing extrachromosomal DNA molecules...
- c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleusSherif F Louis
Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, Canada R3E 0V9
Proc Natl Acad Sci U S A 102:9613-8. 2005..Our findings reveal that c-Myc possesses the ability to structurally modify chromosomes through telomeric fusions, thereby reorganizing the genetic information...
- c-Myc, Genomic Instability and DiseaseF Kuttler
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Canada
Genome Dyn 1:171-90. 2006..We propose that c-Myc is a structural modifier of the genome that affects the nuclear organization and an important molecule in tumor cell progression through the induction of genomic instability...
- Chromosomal rearrangements after ex vivo Epstein-Barr virus (EBV) infection of human B cellsS Lacoste
Department of Physiology, Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
Oncogene 29:503-15. 2010..In conclusion, this study suggests that DNA damage and telomere dysfunction contribute to EBV-related chromosomal instability in early LCLs...
- Alterations of centromere positions in nuclei of immortalized and malignant mouse lymphocytesRahul Sarkar
Manitoba Institute of Cell Biology, The University of Manitoba, CancerCare Manitoba, Winnipeg, Manitoba, Canada
Cytometry A 71:386-92. 2007..However, data on centromere positions during cellular transformation remain elusive. This study has focused on mouse lymphocytes and investigated the centromere positions in primary, immortalized, and tumor cells...
- Fus deficiency in mice results in defective B-lymphocyte development and activation, high levels of chromosomal instability and perinatal deathG G Hicks
Manitoba Institute of Cell Biology and the University of Manitoba, Winnipeg, Canada
Nat Genet 24:175-9. 2000..The involvement of a nuclear riboprotein in these processes in vivo indicates that Fus is important in genome maintenance...
- Premalignant cervical lesions are characterized by dihydrofolate reductase gene amplification and c-Myc overexpression: possible biomarkersFernando B Guijon
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
J Low Genit Tract Dis 11:265-72. 2007..This study analyzes c-Myc protein levels and the stability of the DHFR gene in cervical tissue biopsies...
- Nuclear imaging in three dimensions: a unique tool in cancer researchThomas Klonisch
Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, Manitoba, Canada
Ann Anat 192:292-301. 2010....
- c-Myc-dependent formation of Robertsonian translocation chromosomes in mouse cellsAmanda Guffei
Manitoba Institute of Cell Biology, The University of Manitoba, CancerCare Manitoba, Winnipeg, MB, Canada
Neoplasia 9:578-88. 2007..Our findings are relevant to the understanding of nuclear chromosome remodeling during the initiation of genomic instability and tumorigenesis...
- c-Myc initiates illegitimate replication of the ribonucleotide reductase R2 geneT I Kuschak
Department of Microbiology, Manitoba Institute of Cell Biology, The University of Manitoba, 675 McDermot Ave, Winnipeg, MB, R3E 0V9, Canada
Oncogene 21:909-20. 2002..Thus, c-Myc can act as an illegitimate replication-licensing factor that promotes de novo replication initiation and illegitimate replication timing that adversely impacts upon genomic stability...
- Centromeres in cell division, evolution, nuclear organization and diseaseAmanda Gonçalves Dos Santos Silva
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada
J Cell Biochem 104:2040-58. 2008..Multidisciplinary research will allow for new insights into its normal and aberrant nuclear organization and may allow for new therapeutic interventions that target events linked to centromere function and cell division...
- Increased genomic instability and altered chromosomal protein phosphorylation timing in HRAS-transformed mouse fibroblastsKatherine L Dunn
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
Genes Chromosomes Cancer 48:397-409. 2009..Together our results demonstrate the increased genomic instability and alterations in the epigenetic program in HRAS-transformed cells...
- Formation of non-random extrachromosomal elements during development, differentiation and oncogenesisFabien Kuttler
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, 675 McDermot Avenue, Winnipeg, Man R3E 0V9, Canada
Semin Cancer Biol 17:56-64. 2007..Identification of genes located on EEs will undoubtedly allow a better understanding of genome dynamics and oncogenic pathways...
- c-Myc-induced extrachromosomal elements carry active chromatinGreg Smith
Manitoba Institute of Cell Biology, CancerCare Manitoba, the Genomic Center for Cancer Research and Diagnosis, Winnipeg, Manitoba, Canada
Neoplasia 5:110-20. 2003..In contrast, EEs isolated from non-Myc-activated cells neither carry the DHFR gene nor incorporate BrdU, suggesting that c-Myc deregulation generates a new class of EEs...
- Uncoupling of genomic instability and tumorigenesis in a mouse model of Burkitt's lymphoma expressing a conditional box II-deleted Myc proteinThierry Fest
Manitoba Institute of Cell Biology and The Genomic Center for Cancer Research and Diagnosis, The University of Manitoba, 675 McDermot Avenue, Winnipeg, MB R3E 0V9, Canada
Oncogene 24:2944-53. 2005..Therefore, the genomic instability phenotype induced by Delta106-Myc can be genetically uncoupled from its tumorigenic potential...
- Dynamic chromosomal rearrangements in Hodgkin's lymphoma are due to ongoing three-dimensional nuclear remodeling and breakage-bridge-fusion cyclesAmanda Guffei
Manitoba Institute of Cell Biology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB, Canada
Haematologica 95:2038-46. 2010..We have recently shown telomere dysfunction and aberrant synchronous/asynchronous cell divisions during the transition of Hodgkin cells to Reed-Sternberg cells.1..
- Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocationBrett S Abrahams
Graduate Program in Neuroscience, Department of Medical Genetics, University of Columbia, Vancouver, British Columbia, Canada
Genesis 36:134-41. 2003..This is the first description of a BAC-mediated chromosomal rearrangement and the first application of SKY to identify transgene-induced chromosomal rearrangements...
- ATM-Chk2-p53 activation prevents tumorigenesis at an expense of organ homeostasis upon Brca1 deficiencyLiu Cao
Genetics of Development and Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 25:2167-77. 2006..Our data highlight how accurate maintenance of genomic integrity is critical for the suppression of both aging and malignancy, and provide a further link between aging and cancer...
- c-MYC overexpression in Ba/F3 cells simultaneously elicits genomic instability and apoptosisThierry Fest
Hematology Department, University Hospital Jean Minjoz, 20539 Besançon Cedex, France
Oncogene 21:2981-90. 2002..However, only wt-Myc simultaneously induced genomic instability and apoptosis. Mt-Myc proteins failed to induce apoptosis, thereby generating a strong imbalance towards the survival of genomically unstable cells...
- Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluorescence in-situ hybridization with a subtelomeric probeKatalin Benedek
Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden
Chromosome Res 12:777-85. 2004..Our findings suggest that the Pre-B ABL/MYC cells may be used to analyse the genetic changes affecting chromosome 11 that are associated with v-abl/myc-dependent tumorigenesis in mouse B cells...
- Cell cycle-dependent 3D distribution of telomeres and telomere repeat-binding factor 2 (TRF2) in HaCaT and HaCaT-myc cellsSibylle Ermler
German Cancer Research Center, Heidelberg, Germany
Eur J Cell Biol 83:681-90. 2004..Thus, we propose that TAs, which subsequently also cluster their respective chromosomes, contribute to genomic instability by forcing an abnormal chromosome segregation during mitosis...
- Non-random genomic instability in cancer: a fact, not an illusionSabine Mai
Semin Cancer Biol 17:1-4. 2007
- Tip60 is a haplo-insufficient tumour suppressor required for an oncogene-induced DNA damage responseChiara Gorrini
Department of Experimental Oncology, European Institute of Oncology IEO, IFOM IEO Campus, Milan 20139, Italy
Nature 448:1063-7. 2007..We suggest that this is because critical levels of Tip60 are required for mounting an oncogene-induced DDR in incipient tumour cells, the failure of which might synergize with p53 mutation towards tumour progression...