D W Litchfield

Summary

Affiliation: University of Western Ontario
Country: Canada

Publications

  1. pmc Protein kinase CK2: structure, regulation and role in cellular decisions of life and death
    David W Litchfield
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochem J 369:1-15. 2003
  2. ncbi request reprint CK2 interacting proteins: emerging paradigms for CK2 regulation?
    Mary Ellen K Olsten
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5CI
    Mol Cell Biochem 274:115-24. 2005
  3. pmc The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2beta
    Ashley C Bibby
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    Int J Biol Sci 1:67-79. 2005
  4. pmc Characterizing the convergence of protein kinase CK2 and caspase-3 reveals isoform-specific phosphorylation of caspase-3 by CK2α': implications for pathological roles of CK2 in promoting cancer cell survival
    Jacob P Turowec
    Department of Biochemistry, Schulich School of Medicine, Western University, London, ON, Canada
    Oncotarget 4:560-71. 2013
  5. pmc Unbiased functional proteomics strategy for protein kinase inhibitor validation and identification of bona fide protein kinase substrates: application to identification of EEF1D as a substrate for CK2
    Laszlo Gyenis
    Department of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada
    J Proteome Res 10:4887-901. 2011
  6. pmc Non-catalytic participation of the Pin1 peptidyl-prolyl isomerase domain in target binding
    Brendan T Innes
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario London, ON, Canada
    Front Physiol 4:18. 2013
  7. pmc Development of an unbiased statistical method for the analysis of unigenic evolution
    Colleen D Behrsin
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    BMC Bioinformatics 7:150. 2006
  8. doi request reprint Localization of phosphorylated CK2alpha to the mitotic spindle requires the peptidyl-prolyl isomerase Pin1
    Nicole A St-Denis
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London ON N6A 5C1, Canada
    J Cell Sci 124:2341-8. 2011
  9. ncbi request reprint Functional specialization of CK2 isoforms and characterization of isoform-specific binding partners
    D W Litchfield
    Department of Biochemistry, University of Western Ontario, London, Canada
    Mol Cell Biochem 227:21-9. 2001
  10. ncbi request reprint Identification and characterization of CKIP-1, a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2
    D G Bosc
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 275:14295-306. 2000

Collaborators

  • C Zhang
  • Shawn S C Li
  • M D Cole
  • Christopher J Brandl
  • J R Yates
  • Maria Bretner
  • Laszlo Gyenis
  • R D Feldman
  • Brian H Shilton
  • Timothy Haystead
  • Lee Graves
  • James R Hammond
  • S P Lees-Miller
  • Justin Guy
  • Paul O Hassa
  • Lorenzo A Pinna
  • J R Davie
  • James S Duncan
  • Greg Vilk
  • Mary Ellen K Olsten
  • David A Canton
  • Jacob P Turowec
  • Melanie L Bailey
  • Nicole A St-Denis
  • Piotr Zien
  • Gilles Lajoie
  • Brendan T Innes
  • Arianna Donella-Deana
  • Ryan D Mohan
  • Gregory B Gloor
  • Piya Lahiry
  • N A St-Denis
  • D Richard Derksen
  • Kagan Kerman
  • Henning Kleine
  • Bernhard Luscher
  • Ashley C French
  • C D Behrsin
  • Jane E Weber
  • Ganna Panasyuk
  • Colleen D Behrsin
  • John A Cooper
  • Stefania Sarno
  • Ashley C Bibby
  • Meaghan Stolk
  • Amanda Doherty-Kirby
  • Moira M Messenger
  • Haruo Hanawa
  • Jian Min Sun
  • Michelle Gabriel
  • G Vilk
  • D A Canton
  • C M Tan
  • Erin L Parker
  • D G Bosc
  • Marc Tini
  • Joseph Torchia
  • Kevin A Strauss
  • John F Robinson
  • Mildred B Martens
  • Jian Wang
  • Robert A Hegele
  • Erik G Puffenberger
  • Victoria Siu
  • Matthew B Lanktree
  • David A Ramsay
  • C Anthony Rupar
  • Michael O Hottiger
  • Heinz Bernhard Kraatz
  • Haifeng Song
  • John Lenehan
  • Chenggang Wu
  • Krzysztof Lesniewicz
  • Elzbieta Poreba
  • K S Hamilton
  • K S Bateman
  • L M Wahl
  • M L Bailey
  • Hanspeter Niederstrasser
  • A Saleh
  • Ivan T Gout
  • Alexander Zhyvoloup
  • Lindi M Wahl
  • Valeriy Filonenko
  • Ivan Nemazanyy
  • Elizabeth Cooper
  • David Shugar
  • Kyoungtae Kim
  • Janusz Skierski
  • Paul A Walton

Detail Information

Publications41

  1. pmc Protein kinase CK2: structure, regulation and role in cellular decisions of life and death
    David W Litchfield
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochem J 369:1-15. 2003
    ..Furthermore, this anti-apoptotic function of CK2 may contribute to its ability to participate in transformation and tumorigenesis...
  2. ncbi request reprint CK2 interacting proteins: emerging paradigms for CK2 regulation?
    Mary Ellen K Olsten
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5CI
    Mol Cell Biochem 274:115-24. 2005
    ..A second aspect of this work involves an examination of the structural basis for interactions between CK2 and CKIP-1 with emphasis on a putative HIKE domain in CK2...
  3. pmc The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2beta
    Ashley C Bibby
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    Int J Biol Sci 1:67-79. 2005
    ....
  4. pmc Characterizing the convergence of protein kinase CK2 and caspase-3 reveals isoform-specific phosphorylation of caspase-3 by CK2α': implications for pathological roles of CK2 in promoting cancer cell survival
    Jacob P Turowec
    Department of Biochemistry, Schulich School of Medicine, Western University, London, ON, Canada
    Oncotarget 4:560-71. 2013
    ....
  5. pmc Unbiased functional proteomics strategy for protein kinase inhibitor validation and identification of bona fide protein kinase substrates: application to identification of EEF1D as a substrate for CK2
    Laszlo Gyenis
    Department of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada
    J Proteome Res 10:4887-901. 2011
    ..Furthermore, this validation strategy could be adaptable to other protein kinases and readily combined with other phosphoproteomic methods...
  6. pmc Non-catalytic participation of the Pin1 peptidyl-prolyl isomerase domain in target binding
    Brendan T Innes
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario London, ON, Canada
    Front Physiol 4:18. 2013
    ..We also compare sequences that are recognized by Pin1 within its individual interaction partners to investigate the underlying basis for its different types of interactions...
  7. pmc Development of an unbiased statistical method for the analysis of unigenic evolution
    Colleen D Behrsin
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    BMC Bioinformatics 7:150. 2006
    ..In addition, it was unclear when sufficient functional variants had been examined to conclude that residues conserved in all variants have important functional roles...
  8. doi request reprint Localization of phosphorylated CK2alpha to the mitotic spindle requires the peptidyl-prolyl isomerase Pin1
    Nicole A St-Denis
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London ON N6A 5C1, Canada
    J Cell Sci 124:2341-8. 2011
    ..Furthermore, because Pin1 has many important spindle substrates, this might represent a general mechanism for localization of mitotic signalling proteins...
  9. ncbi request reprint Functional specialization of CK2 isoforms and characterization of isoform-specific binding partners
    D W Litchfield
    Department of Biochemistry, University of Western Ontario, London, Canada
    Mol Cell Biochem 227:21-9. 2001
    ....
  10. ncbi request reprint Identification and characterization of CKIP-1, a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2
    D G Bosc
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 275:14295-306. 2000
    ..We postulate that CKIP-1 is a non-enzymatic regulator of one isoform of CK2 (i.e. CK2alpha) with a potential role in targeting CK2alpha to a particular cellular location...
  11. pmc Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK2beta
    D A Canton
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Biochem J 358:87-94. 2001
    ....
  12. ncbi request reprint Functionally important residues in the peptidyl-prolyl isomerase Pin1 revealed by unigenic evolution
    C D Behrsin
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    J Mol Biol 365:1143-62. 2007
    ..Accordingly, an alternative catalytic mechanism for Pin1 is postulated...
  13. ncbi request reprint Inducible expression of the regulatory protein kinase CK2beta subunit: incorporation into complexes with catalytic CK2 subunits and re-examination of the effects of CK2beta on cell proliferation
    G Vilk
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    J Cell Biochem 84:84-99. 2001
    ..Furthermore, these results indicate that increased expression of CK2beta is not by itself sufficient to effect alterations in cell proliferation...
  14. doi request reprint Protein kinase CK2 in health and disease: From birth to death: the role of protein kinase CK2 in the regulation of cell proliferation and survival
    N A St-Denis
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Cell Mol Life Sci 66:1817-29. 2009
    ..Finally, we discuss possible ways in which these functions are integrated to allow the cell to respond appropriately in the presence of multiple signals...
  15. ncbi request reprint Tyrosine kinase-mediated serine phosphorylation of adenylyl cyclase
    C M Tan
    The John P Robarts Research Institute, London, Ontario, Canada, N6A 5K8
    Biochemistry 40:1702-9. 2001
    ..Together, these data define a novel tyrosine kinase-mediated mechanism leading to serine phosphorylation of adenylyl cyclase isoform VI and the sensitization of adenylyl cyclase responsiveness...
  16. ncbi request reprint Interactions between protein kinase CK2 and Pin1. Evidence for phosphorylation-dependent interactions
    Moira M Messenger
    Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 277:23054-64. 2002
    ..Furthermore, these results provide new insights into the functional role of the mitotic phosphorylation of CK2 and provide a new mechanism for selectively regulating the ability of CK2 to phosphorylate one of its mitotic targets...
  17. doi request reprint Protein kinase CK2 catalyzes tyrosine phosphorylation in mammalian cells
    Greg Vilk
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Cell Signal 20:1942-51. 2008
    ..Furthermore, these results raise the possibility that increased CK2 levels that frequently accompany transformation may contribute to the increased tyrosine phosphorylation that occurs in transformed cells...
  18. ncbi request reprint Phosphorylation regulates the stability of the regulatory CK2beta subunit
    Cunjie Zhang
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Oncogene 21:3754-64. 2002
    ..Furthermore, based on these results, we speculate that alterations in the phosphorylation or dephosphorylation of the regulatory CK2beta subunit could underlie the elevated expression of CK2 that is observed in cancer cells...
  19. ncbi request reprint The Pleckstrin homology domain of CK2 interacting protein-1 is required for interactions and recruitment of protein kinase CK2 to the plasma membrane
    Mary Ellen K Olsten
    Departments of Biochemistry and Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 279:42114-27. 2004
    ..Overall, these results provide novel insights into how CK2, a predominantly nuclear enzyme, is targeted to the plasma membrane, and perhaps more importantly how it may be regulated...
  20. pmc Subtype-specific regulation of equilibrative nucleoside transporters by protein kinase CK2
    Meaghan Stolk
    Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochem J 386:281-9. 2005
    ....
  21. ncbi request reprint Tra1p is a component of the yeast Ada.Spt transcriptional regulatory complexes
    A Saleh
    Department of Biochemistry, University of Western Ontario, London N6A 5C1, Canada
    J Biol Chem 273:26559-65. 1998
    ..Despite the similarity of Tra1p to a group of putative protein kinases, we have not detected protein kinase activity within immunoprecipitates of Tra1p or the Ada.Spt complexes...
  22. doi request reprint Peptide biosensors for the electrochemical measurement of protein kinase activity
    Kagan Kerman
    Department of Chemistry, Faculty of Science, The University of Western Ontario, London, Ontario, Canada N6A 5B7
    Anal Chem 80:9395-401. 2008
    ..Peptide modified electrochemical biosensors are promising candidates for cost-effective in vitro kinase activity and inhibitor screening assays...
  23. doi request reprint The dual histidine motif in the active site of Pin1 has a structural rather than catalytic role
    Melanie L Bailey
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochemistry 47:11481-9. 2008
    ..Instead, we suggest these histidines play a key role in domain structure or stability...
  24. pmc Evidence for regulation of mitotic progression through temporal phosphorylation and dephosphorylation of CK2alpha
    Nicole A St-Denis
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Mol Cell Biol 29:2068-81. 2009
    ..Collectively, these studies indicate that reversible phosphorylation of CK2alpha requires precise regulation to allow proper mitotic progression...
  25. ncbi request reprint Order or chaos? An evaluation of the regulation of protein kinase CK2
    Mary Ellen K Olsten
    Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, ON N6A 5C1, Canada
    Biochem Cell Biol 82:681-93. 2004
    ....
  26. pmc The pleckstrin homology domain-containing protein CKIP-1 is involved in regulation of cell morphology and the actin cytoskeleton and interaction with actin capping protein
    David A Canton
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Mol Cell Biol 25:3519-34. 2005
    ..Overall, our results are consistent with CKIP-1 playing a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein...
  27. pmc The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein
    David A Canton
    Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 281:36347-59. 2006
    ....
  28. ncbi request reprint A novel costimulatory signaling in human T lymphocytes by a splice variant of CD28
    Haruo Hanawa
    Department of Microbiology and Immunology, The John P Robarts Research Institute, University of Western Ontario, 1400 Western Road, London, Ontario, Canada N6G 2V4
    Blood 99:2138-45. 2002
    ..These data demonstrate that CD28i, via noncovalent association with CD28, plays a role as a costimulatory signal amplifier in human T cells...
  29. pmc Opposing regulatory roles of phosphorylation and acetylation in DNA mispair processing by thymine DNA glycosylase
    Ryan D Mohan
    Department of Physiology and Pharmacology, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Nucleic Acids Res 38:1135-48. 2010
    ....
  30. doi request reprint Regulation of cell proliferation and survival: convergence of protein kinases and caspases
    James S Duncan
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario Canada N6A 5C1
    Biochim Biophys Acta 1804:505-10. 2010
    ....
  31. ncbi request reprint The shape of things to come: an emerging role for protein kinase CK2 in the regulation of cell morphology and the cytoskeleton
    David A Canton
    Regulatory Biology and Functional Genomics Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Cell Signal 18:267-75. 2006
    ....
  32. pmc A multiplex human syndrome implicates a key role for intestinal cell kinase in development of central nervous, skeletal, and endocrine systems
    Piya Lahiry
    Robarts Research Institute, London, Ontario N6A 5K8, Canada
    Am J Hum Genet 84:134-47. 2009
    ..We also demonstrate that the R272Q mutant fails to localize at the nucleus and has diminished kinase activity. These findings suggest that ICK plays a key role in the development of multiple organ systems...
  33. ncbi request reprint Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2
    Piotr Zien
    Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochim Biophys Acta 1754:271-80. 2005
    ..Finally, development of shRNA strategies for the selective knockdown of the CK2alpha and CK2alpha' isoforms reinforces the foregoing results, indicating that inhibition of CK2 leads to attenuation of proliferation...
  34. ncbi request reprint The emerging CK2 interactome: insights into the regulation and functions of CK2
    Laszlo Gyenis
    Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada, N6A 5C1
    Mol Cell Biochem 316:5-14. 2008
    ....
  35. doi request reprint An unbiased evaluation of CK2 inhibitors by chemoproteomics: characterization of inhibitor effects on CK2 and identification of novel inhibitor targets
    James S Duncan
    Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    Mol Cell Proteomics 7:1077-88. 2008
    ..The results described in the present study provide insight into the molecular mechanism of action of the inhibitors as well as drug specificity that will assist in the development of more specific next generation CK2 inhibitors...
  36. ncbi request reprint Too much of a good thing: the role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2
    James S Duncan
    Department of Biochemistry, The University of Western Ontario, London, ON, Canada
    Biochim Biophys Acta 1784:33-47. 2008
    ..This review will focus on published evidence highlighting the molecular mechanisms by which CK2 functions in the promotion of tumorigenesis, as well as review current strategies being used to inhibit CK2...
  37. ncbi request reprint Development of a stabilized form of the regulatory CK2beta subunit that inhibits cell proliferation
    Ashley C French
    Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 282:29667-77. 2007
    ..Overall, our results indicate that a stabilized form of CK2beta can be used to inhibit cell proliferation...
  38. ncbi request reprint Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at Ser-17
    Ganna Panasyuk
    Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv 03143, Ukraine
    J Biol Chem 281:31188-201. 2006
    ..Taken together, this study establishes a functional link between S6K1 II and CK2 signaling, which involves the regulation of S6K1 II nuclear export by CK2-mediated phosphorylation of Ser-17...
  39. pmc Tyrosine phosphorylation of protein kinase CK2 by Src-related tyrosine kinases correlates with increased catalytic activity
    Arianna Donella-Deana
    Dipartimento di Chimica Biologica and CRIBI, Centro Nazionale delle Ricerche, Institute of Neuroscience, University of Padova, Viale G Colombo 3, Italy
    Biochem J 372:841-9. 2003
    ..A CK2alpha mutant in which Tyr(255) was replaced by phenylalanine proved less susceptible to phosphorylation and refractory to stimulation by c-Fgr...
  40. ncbi request reprint The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2
    Jian Min Sun
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 277:35783-6. 2002
    ..CK2 phosphorylation of HDAC2 recruited by Sp1 or Sp3 could regulate HDAC activity and alter the balance of histone deacetylase and histone acetyltransferase activities and dynamic chromatin remodeling of estrogen-regulated genes...
  41. doi request reprint Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation
    Henning Kleine
    Institut fur Biochemie und Molekularbiologie, Klinikum, RWTH Aachen University, 52057 Aachen, Germany
    Mol Cell 32:57-69. 2008
    ..This mechanism explains why the novel PARPs are unable to function as polymerases. This discovery will help to illuminate the different biological functions of mono- versus poly-ADP-ribosylation in cells...