Research Topics
| D W LitchfieldSummaryAffiliation: University of Western Ontario Country: Canada Publications
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Detail Information
Publications
Protein kinase CK2: structure, regulation and role in cellular decisions of life and deathDavid W Litchfield
Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5C1
Biochem J 369:1-15. 2003..Furthermore, this anti-apoptotic function of CK2 may contribute to its ability to participate in transformation and tumorigenesis...
CK2 interacting proteins: emerging paradigms for CK2 regulation?Mary Ellen K Olsten
Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5CI
Mol Cell Biochem 274:115-24. 2005..A second aspect of this work involves an examination of the structural basis for interactions between CK2 and CKIP-1 with emphasis on a putative HIKE domain in CK2...- Development of an unbiased statistical method for the analysis of unigenic evolutionColleen D Behrsin
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
BMC Bioinformatics 7:150. 2006..In addition, it was unclear when sufficient functional variants had been examined to conclude that residues conserved in all variants have important functional roles... 
Localization of phosphorylated CK2alpha to the mitotic spindle requires the peptidyl-prolyl isomerase Pin1Nicole A St-Denis
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London ON N6A 5C1, Canada
J Cell Sci 124:2341-8. 2011..Furthermore, because Pin1 has many important spindle substrates, this might represent a general mechanism for localization of mitotic signalling proteins...- Functional specialization of CK2 isoforms and characterization of isoform-specific binding partnersD W Litchfield
Department of Biochemistry, University of Western Ontario, London, Canada
Mol Cell Biochem 227:21-9. 2001.... - Identification and characterization of CKIP-1, a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2D G Bosc
Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 275:14295-306. 2000..We postulate that CKIP-1 is a non-enzymatic regulator of one isoform of CK2 (i.e. CK2alpha) with a potential role in targeting CK2alpha to a particular cellular location... - Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK2betaD A Canton
Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
Biochem J 358:87-94. 2001.... - Inducible expression of the regulatory protein kinase CK2beta subunit: incorporation into complexes with catalytic CK2 subunits and re-examination of the effects of CK2beta on cell proliferationG Vilk
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1
J Cell Biochem 84:84-99. 2001..Furthermore, these results indicate that increased expression of CK2beta is not by itself sufficient to effect alterations in cell proliferation... - Functionally important residues in the peptidyl-prolyl isomerase Pin1 revealed by unigenic evolutionC D Behrsin
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
J Mol Biol 365:1143-62. 2007..Accordingly, an alternative catalytic mechanism for Pin1 is postulated... - The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 independent roles reveal a secret identity for CK2betaAshley C Bibby
Department of Biochemistry, Siebens-Drake Research Institute, University of Western Ontario, London, Ontario, Canada, N6A 5C1
Int J Biol Sci 1:67-79. 2005.... - Tyrosine kinase-mediated serine phosphorylation of adenylyl cyclaseC M Tan
The John P Robarts Research Institute, London, Ontario, Canada, N6A 5K8
Biochemistry 40:1702-9. 2001..Together, these data define a novel tyrosine kinase-mediated mechanism leading to serine phosphorylation of adenylyl cyclase isoform VI and the sensitization of adenylyl cyclase responsiveness... - Protein kinase CK2 in health and disease: From birth to death: the role of protein kinase CK2 in the regulation of cell proliferation and survivalN A St-Denis
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
Cell Mol Life Sci 66:1817-29. 2009..Finally, we discuss possible ways in which these functions are integrated to allow the cell to respond appropriately in the presence of multiple signals... - Interactions between protein kinase CK2 and Pin1. Evidence for phosphorylation-dependent interactionsMoira M Messenger
Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 277:23054-64. 2002..Furthermore, these results provide new insights into the functional role of the mitotic phosphorylation of CK2 and provide a new mechanism for selectively regulating the ability of CK2 to phosphorylate one of its mitotic targets... - Protein kinase CK2 catalyzes tyrosine phosphorylation in mammalian cellsGreg Vilk
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Cell Signal 20:1942-51. 2008..Furthermore, these results raise the possibility that increased CK2 levels that frequently accompany transformation may contribute to the increased tyrosine phosphorylation that occurs in transformed cells... - Phosphorylation regulates the stability of the regulatory CK2beta subunitCunjie Zhang
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Oncogene 21:3754-64. 2002..Furthermore, based on these results, we speculate that alterations in the phosphorylation or dephosphorylation of the regulatory CK2beta subunit could underlie the elevated expression of CK2 that is observed in cancer cells... - The Pleckstrin homology domain of CK2 interacting protein-1 is required for interactions and recruitment of protein kinase CK2 to the plasma membraneMary Ellen K Olsten
Departments of Biochemistry and Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 279:42114-27. 2004..Overall, these results provide novel insights into how CK2, a predominantly nuclear enzyme, is targeted to the plasma membrane, and perhaps more importantly how it may be regulated... - Subtype-specific regulation of equilibrative nucleoside transporters by protein kinase CK2Meaghan Stolk
Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada N6A 5C1
Biochem J 386:281-9. 2005.... - The dual histidine motif in the active site of Pin1 has a structural rather than catalytic roleMelanie L Bailey
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Biochemistry 47:11481-9. 2008..Instead, we suggest these histidines play a key role in domain structure or stability... - Peptide biosensors for the electrochemical measurement of protein kinase activityKagan Kerman
Department of Chemistry, Faculty of Science, The University of Western Ontario, London, Ontario, Canada N6A 5B7
Anal Chem 80:9395-401. 2008..Peptide modified electrochemical biosensors are promising candidates for cost-effective in vitro kinase activity and inhibitor screening assays... - Evidence for regulation of mitotic progression through temporal phosphorylation and dephosphorylation of CK2alphaNicole A St-Denis
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
Mol Cell Biol 29:2068-81. 2009..Collectively, these studies indicate that reversible phosphorylation of CK2alpha requires precise regulation to allow proper mitotic progression... - Order or chaos? An evaluation of the regulation of protein kinase CK2Mary Ellen K Olsten
Department of Biochemistry, Siebens Drake Research Institute, University of Western Ontario, London, ON N6A 5C1, Canada
Biochem Cell Biol 82:681-93. 2004.... - The pleckstrin homology domain-containing protein CKIP-1 is involved in regulation of cell morphology and the actin cytoskeleton and interaction with actin capping proteinDavid A Canton
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Mol Cell Biol 25:3519-34. 2005..Overall, our results are consistent with CKIP-1 playing a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein... - Tra1p is a component of the yeast Ada.Spt transcriptional regulatory complexesA Saleh
Department of Biochemistry, University of Western Ontario, London N6A 5C1, Canada
J Biol Chem 273:26559-65. 1998..Despite the similarity of Tra1p to a group of putative protein kinases, we have not detected protein kinase activity within immunoprecipitates of Tra1p or the Ada.Spt complexes... - The shape of things to come: an emerging role for protein kinase CK2 in the regulation of cell morphology and the cytoskeletonDavid A Canton
Regulatory Biology and Functional Genomics Group, Siebens-Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Cell Signal 18:267-75. 2006.... - Opposing regulatory roles of phosphorylation and acetylation in DNA mispair processing by thymine DNA glycosylaseRyan D Mohan
Department of Physiology and Pharmacology, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
Nucleic Acids Res 38:1135-48. 2010.... - Regulation of cell proliferation and survival: convergence of protein kinases and caspasesJames S Duncan
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario Canada N6A 5C1
Biochim Biophys Acta 1804:505-10. 2010.... - A novel costimulatory signaling in human T lymphocytes by a splice variant of CD28Haruo Hanawa
Department of Microbiology and Immunology, The John P Robarts Research Institute, University of Western Ontario, 1400 Western Road, London, Ontario, Canada N6G 2V4
Blood 99:2138-45. 2002..These data demonstrate that CD28i, via noncovalent association with CD28, plays a role as a costimulatory signal amplifier in human T cells... - A multiplex human syndrome implicates a key role for intestinal cell kinase in development of central nervous, skeletal, and endocrine systemsPiya Lahiry
Robarts Research Institute, London, Ontario N6A 5K8, Canada
Am J Hum Genet 84:134-47. 2009..We also demonstrate that the R272Q mutant fails to localize at the nucleus and has diminished kinase activity. These findings suggest that ICK plays a key role in the development of multiple organ systems... - Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2Piotr Zien
Regulatory Biology and Functional Genomics Research Group, Siebens-Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
Biochim Biophys Acta 1754:271-80. 2005..Finally, development of shRNA strategies for the selective knockdown of the CK2alpha and CK2alpha' isoforms reinforces the foregoing results, indicating that inhibition of CK2 leads to attenuation of proliferation... - The role of CKIP-1 in cell morphology depends on its interaction with actin-capping proteinDavid A Canton
Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 281:36347-59. 2006.... - The emerging CK2 interactome: insights into the regulation and functions of CK2Laszlo Gyenis
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada, N6A 5C1
Mol Cell Biochem 316:5-14. 2008.... - An unbiased evaluation of CK2 inhibitors by chemoproteomics: characterization of inhibitor effects on CK2 and identification of novel inhibitor targetsJames S Duncan
Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
Mol Cell Proteomics 7:1077-88. 2008..The results described in the present study provide insight into the molecular mechanism of action of the inhibitors as well as drug specificity that will assist in the development of more specific next generation CK2 inhibitors... - Too much of a good thing: the role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2James S Duncan
Department of Biochemistry, The University of Western Ontario, London, ON, Canada
Biochim Biophys Acta 1784:33-47. 2008..This review will focus on published evidence highlighting the molecular mechanisms by which CK2 functions in the promotion of tumorigenesis, as well as review current strategies being used to inhibit CK2... - Development of a stabilized form of the regulatory CK2beta subunit that inhibits cell proliferationAshley C French
Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 282:29667-77. 2007..Overall, our results indicate that a stabilized form of CK2beta can be used to inhibit cell proliferation... - Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at Ser-17Ganna Panasyuk
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv 03143, Ukraine
J Biol Chem 281:31188-201. 2006..Taken together, this study establishes a functional link between S6K1 II and CK2 signaling, which involves the regulation of S6K1 II nuclear export by CK2-mediated phosphorylation of Ser-17... - Tyrosine phosphorylation of protein kinase CK2 by Src-related tyrosine kinases correlates with increased catalytic activityArianna Donella-Deana
Dipartimento di Chimica Biologica and CRIBI, Centro Nazionale delle Ricerche, Institute of Neuroscience, University of Padova, Viale G. Colombo 3, Italy
Biochem J 372:841-9. 2003..A CK2alpha mutant in which Tyr(255) was replaced by phenylalanine proved less susceptible to phosphorylation and refractory to stimulation by c-Fgr... - The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2Jian Min Sun
Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
J Biol Chem 277:35783-6. 2002..CK2 phosphorylation of HDAC2 recruited by Sp1 or Sp3 could regulate HDAC activity and alter the balance of histone deacetylase and histone acetyltransferase activities and dynamic chromatin remodeling of estrogen-regulated genes... - Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylationHenning Kleine
Institut fur Biochemie und Molekularbiologie, Klinikum, RWTH Aachen University, 52057 Aachen, Germany
Mol Cell 32:57-69. 2008..This mechanism explains why the novel PARPs are unable to function as polymerases. This discovery will help to illuminate the different biological functions of mono- versus poly-ADP-ribosylation in cells...