Susan P Lees-Miller

Summary

Affiliation: University of Calgary
Country: Canada

Publications

  1. ncbi request reprint DNA damage-induced activation of ATM and ATM-dependent signaling pathways
    Ebba U Kurz
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, Canada
    DNA Repair (Amst) 3:889-900. 2004
  2. pmc Phosphorylation of polynucleotide kinase/ phosphatase by DNA-dependent protein kinase and ataxia-telangiectasia mutated regulates its association with sites of DNA damage
    Angela E Zolner
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    Nucleic Acids Res 39:9224-37. 2011
  3. pmc Enhanced cytotoxicity of PARP inhibition in mantle cell lymphoma harbouring mutations in both ATM and p53
    Chris T Williamson
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    EMBO Mol Med 4:515-27. 2012
  4. doi request reprint PIKK-ing a new partner: a new role for PKB in the DNA damage response
    Susan P Lees-Miller
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, AB T2N 4N1, Canada
    Cancer Cell 13:379-80. 2008
  5. ncbi request reprint Repair of DNA double strand breaks by non-homologous end joining
    S P Lees-Miller
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, NW, Calgary, Alta, Canada T2N 4N1
    Biochimie 85:1161-73. 2003
  6. ncbi request reprint Dysfunction of lamin A triggers a DNA damage response and cellular senescence
    Susan P Lees-Miller
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alta, Canada T2N 4N1
    DNA Repair (Amst) 5:286-9. 2006
  7. ncbi request reprint Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species
    Ebba U Kurz
    Cancer Biology Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:53272-81. 2004
  8. pmc Protein phosphatase 6 interacts with the DNA-dependent protein kinase catalytic subunit and dephosphorylates gamma-H2AX
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada
    Mol Cell Biol 30:1368-81. 2010
  9. pmc Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A
    Aaron A Goodarzi
    Department of Biological Sciences, University of Calgary, Calgary, AB, Canada
    EMBO J 23:4451-61. 2004
  10. pmc Dual modes of interaction between XRCC4 and polynucleotide kinase/phosphatase: implications for nonhomologous end joining
    Rajam S Mani
    Department of Oncology, University of Alberta, Alberta, and the Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada
    J Biol Chem 285:37619-29. 2010

Collaborators

Detail Information

Publications61

  1. ncbi request reprint DNA damage-induced activation of ATM and ATM-dependent signaling pathways
    Ebba U Kurz
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, Canada
    DNA Repair (Amst) 3:889-900. 2004
    ....
  2. pmc Phosphorylation of polynucleotide kinase/ phosphatase by DNA-dependent protein kinase and ataxia-telangiectasia mutated regulates its association with sites of DNA damage
    Angela E Zolner
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    Nucleic Acids Res 39:9224-37. 2011
    ..Together, our results reveal that IR-induced phosphorylation of PNKP by ATM and DNA-PK regulates PNKP function at DSBs...
  3. pmc Enhanced cytotoxicity of PARP inhibition in mantle cell lymphoma harbouring mutations in both ATM and p53
    Chris T Williamson
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    EMBO Mol Med 4:515-27. 2012
    ....
  4. doi request reprint PIKK-ing a new partner: a new role for PKB in the DNA damage response
    Susan P Lees-Miller
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, AB T2N 4N1, Canada
    Cancer Cell 13:379-80. 2008
    ....
  5. ncbi request reprint Repair of DNA double strand breaks by non-homologous end joining
    S P Lees-Miller
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, NW, Calgary, Alta, Canada T2N 4N1
    Biochimie 85:1161-73. 2003
    ..This review discusses how these and other accessory proteins may function in the repair of DSBs produced by ionizing radiation (IR) and by V(D)J recombination...
  6. ncbi request reprint Dysfunction of lamin A triggers a DNA damage response and cellular senescence
    Susan P Lees-Miller
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alta, Canada T2N 4N1
    DNA Repair (Amst) 5:286-9. 2006
    ..These studies underscore the role of the nuclear envelope in maintaining genomic stability and the interplay between nuclear architecture and the DNA damage response...
  7. ncbi request reprint Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species
    Ebba U Kurz
    Cancer Biology Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:53272-81. 2004
    ....
  8. pmc Protein phosphatase 6 interacts with the DNA-dependent protein kinase catalytic subunit and dephosphorylates gamma-H2AX
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada
    Mol Cell Biol 30:1368-81. 2010
    ..We propose that a novel function of DNA-PKcs is to recruit PP6 to sites of DNA damage and that PP6 contributes to the dephosphorylation of gamma-H2AX, the dissolution of IR-induced foci, and release from the G(2)/M checkpoint in vivo...
  9. pmc Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A
    Aaron A Goodarzi
    Department of Biological Sciences, University of Calgary, Calgary, AB, Canada
    EMBO J 23:4451-61. 2004
    ..We propose that PP2A plays an important role in the regulation of ATM autophosphorylation and activity in vivo...
  10. pmc Dual modes of interaction between XRCC4 and polynucleotide kinase/phosphatase: implications for nonhomologous end joining
    Rajam S Mani
    Department of Oncology, University of Alberta, Alberta, and the Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada
    J Biol Chem 285:37619-29. 2010
    ..Our results reveal that CK2-mediated phosphorylation of XRCC4 can have different effects on PNKP activity, with implications for the roles of XRCC4 and PNKP in NHEJ...
  11. pmc DNA-PK and ATM phosphorylation sites in XLF/Cernunnos are not required for repair of DNA double strand breaks
    Yaping Yu
    Department of Biochemistry and Molecular Biology, and The Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada
    DNA Repair (Amst) 7:1680-92. 2008
    ..We conclude that phosphorylation of XLF at these sites does not play a major role in the repair of IR-induced DSBs in vivo...
  12. pmc The DNA-dependent protein kinase catalytic subunit is phosphorylated in vivo on threonine 3950, a highly conserved amino acid in the protein kinase domain
    Pauline Douglas
    Department of Biochemistry, University of Calgary, Calgary, Alberta, Canada
    Mol Cell Biol 27:1581-91. 2007
    ..Together, these data suggest that threonine 3950 is a functionally important, DNA damage-inducible phosphorylation site and that phosphorylation of this site regulates the activity of DNA-PKcs...
  13. pmc Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase
    Pauline Douglas
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, Calgary, AB, T2N 4N1, Canada
    Biochem J 368:243-51. 2002
    ..Moreover, we show that DNA-PKcs is phosphorylated in vivo at Thr2609, Ser2612, Thr2638 and Thr2647 in okadaic acid-treated human cells. We propose that phosphorylation of these sites may play an important role in DNA-PK function...
  14. ncbi request reprint The DNA-dependent protein kinase interacts with DNA to form a protein-DNA complex that is disrupted by phosphorylation
    Dennis Merkle
    Department of Biochemistry and Molecular Biology, University of Calgary, 3300 Hospital Drive, Calgary AB, T2N 4N1, Canada
    Biochemistry 41:12706-14. 2002
    ..We propose that autophosphorylation of DNA-PK plays an important regulatory role in DNA double-strand break repair by regulating the assembly and disassembly of the DNA-PK-DNA complex...
  15. pmc ATM deficiency sensitizes mantle cell lymphoma cells to poly(ADP-ribose) polymerase-1 inhibitors
    Chris T Williamson
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    Mol Cancer Ther 9:347-57. 2010
    ..Thus, PARP inhibitors have therapeutic potential in the treatment of MCL, and the concept of synthetic lethality extends to human cancers with ATM alterations...
  16. ncbi request reprint DNA-PK-dependent phosphorylation of Ku70/80 is not required for non-homologous end joining
    Pauline Douglas
    Department of Biochemistry and Molecular Biology and Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive N W, Calgary, Alta, Canada T2N 4N1
    DNA Repair (Amst) 4:1006-18. 2005
    ....
  17. pmc Autophosphorylation-dependent remodeling of the DNA-dependent protein kinase catalytic subunit regulates ligation of DNA ends
    Wesley D Block
    Cancer Biology Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    Nucleic Acids Res 32:4351-7. 2004
    ..We propose that autophosphorylation of DNA-PKcs at this cluster of sites is important for remodeling of DNA-PK complexes at DNA ends prior to DNA end joining...
  18. ncbi request reprint DNA-PK phosphorylation sites in XRCC4 are not required for survival after radiation or for V(D)J recombination
    Yaping Yu
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, NW, Calgary, Alta, Canada T2N 1N4
    DNA Repair (Amst) 2:1239-52. 2003
    ....
  19. ncbi request reprint Biochemical characterization of the ataxia-telangiectasia mutated (ATM) protein from human cells
    Aaron A Goodarzi
    Departments of Biochemistry and Molecular Biology, and Biological Sciences, Cancer Biology Research Group, University of Calgary, 3330 Hospital Drive N W, Calgary, AB, Canada
    DNA Repair (Amst) 3:753-67. 2004
    ..Our results suggest that phosphorylation of serine 1981 alone may not directly regulate the subunit composition of ATM...
  20. pmc The human telomerase RNA component, hTR, activates the DNA-dependent protein kinase to phosphorylate heterogeneous nuclear ribonucleoprotein A1
    Nicholas S Y Ting
    Department of Biochemistry and Molecular Biology and Department of Oncology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive N W Calgary, AB T2N 4N1, Canada
    Nucleic Acids Res 37:6105-15. 2009
    ..These data are the first to report that hTR stimulates the kinase activity of DNA-PK toward a known telomere-associated protein, and may provide further insights into the function of DNA-PK at telomeres...
  21. ncbi request reprint Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated
    Aaron A Goodarzi
    Southern Alberta Cancer Research Institute and Department of Biochemistry and Molecular Biology, University of Calgary, AB, Canada
    Methods Mol Biol 365:47-59. 2007
    ..Here, we describe the series of experiments that employed protein phosphatase inhibitors to establish that ATM was regulated by a type-2A protein phosphatase...
  22. pmc XRCC4 and XLF form long helical protein filaments suitable for DNA end protection and alignment to facilitate DNA double strand break repair
    Brandi L Mahaney
    Department of Biochemistry, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada
    Biochem Cell Biol 91:31-41. 2013
    ....
  23. pmc Phosphatidyl inositol 3-kinase-like serine/threonine protein kinases (PIKKs) are required for DNA damage-induced phosphorylation of the 32 kDa subunit of replication protein A at threonine 21
    Wesley D Block
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    Nucleic Acids Res 32:997-1005. 2004
    ..Thus, the regulation of RPA32 Thr21 phosphorylation by multiple DNA damage response protein kinases suggests that Thr21 phosphorylation of RPA32 is a crucial step within the DNA damage response...
  24. ncbi request reprint The isoflavonoids genistein and quercetin activate different stress signaling pathways as shown by analysis of site-specific phosphorylation of ATM, p53 and histone H2AX
    Ruiqiong Ye
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, N W, Calgary, Alta, Canada T2N 4N1
    DNA Repair (Amst) 3:235-44. 2004
    ....
  25. ncbi request reprint Analysis of DNA-dependent protein kinase-mediated DNA end joining by two-photon fluorescence cross-correlation spectroscopy
    Dennis Merkle
    Department of Chemistry, University of Calgary, 2500 University Drive N W, Calgary, Alberta, T2N 1N4, Canada
    Biochemistry 45:4164-72. 2006
    ....
  26. pmc Selective inhibition of the DNA-dependent protein kinase (DNA-PK) by the radiosensitizing agent caffeine
    Wesley D Block
    Department of Biological Sciences, Cancer Biology Research Group, University of Calgary, 3330 Hospital Drive N W, Calgary AB, T2N 4N1, Canada
    Nucleic Acids Res 32:1967-72. 2004
    ..These data suggest that while DNA-PK does not appear to be the target of caffeine-induced radiosensitization, caffeine cannot be used to differentiate between ATM, ATR and DNA- PK-dependent substrate phosphorylation in vivo...
  27. doi request reprint Comparing ERCC1 protein expression, mRNA levels, and genotype in squamous cell carcinomas of the head and neck treated with concurrent chemoradiation stratified by HPV status
    Desiree Hao
    Department of Medical Oncology, Tom Baker Cancer Centre and University of Calgary, Calgary, Alberta, Canada
    Head Neck 34:785-91. 2012
    ..Previous studies examining excision repair cross-complementation group 1 (ERCC1) in squamous cell carcinoma of the head and neck (SCCHN) have not compared methods of ERCC1 testing nor been stratified by human papillomavirus (HPV) status...
  28. pmc Targeting protein for xenopus kinesin-like protein 2 (TPX2) regulates γ-histone 2AX (γ-H2AX) levels upon ionizing radiation
    Gernot Neumayer
    Department of Clinical Neurosciences, Hotchkiss Brain Institute, University of Calgary, Calgary T2N4N1, Canada
    J Biol Chem 287:42206-22. 2012
    ..In sum, our study identifies a novel and the first nuclear function for TPX2 in the cellular responses to DNA damage...
  29. doi request reprint The significance of tumoral ERCC1 status in patients with locally advanced cervical cancer treated with chemoradiation therapy: a multicenter clinicopathologic analysis
    Corinne M Doll
    Department of Oncology, University of Calgary, Calgary, AB, Canada
    Int J Radiat Oncol Biol Phys 85:721-7. 2013
    ....
  30. ncbi request reprint The role of ATM and ATR in DNA damage-induced cell cycle control
    Aaron A Goodarzi
    Departments of Biochemistry and Molecular Biology and Biological Sciences, University of Calgary, 2500 University Drive, N W, Calgary, AB T2N 1N4, Canada
    Prog Cell Cycle Res 5:393-411. 2003
    ..This review focuses on the role of ATM and ATR in various DNA damage response pathways, and discusses the potential for targeting these pathways for the development of novel therapeutics...
  31. doi request reprint Estrogen receptor α-mediated transcription induces cell cycle-dependent DNA double-strand breaks
    Laura M Williamson
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada
    Carcinogenesis 32:279-85. 2011
    ....
  32. doi request reprint Prognostic significance of p16 in locally advanced squamous cell carcinoma of the head and neck treated with concurrent cisplatin and radiotherapy
    Harold Y Lau
    Department of Radiation Oncology, Tom Baker Cancer Centre, Calgary, Alberta, Canada
    Head Neck 33:251-6. 2011
    ..Human papillomavirus (HPV)-related squamous cell cancer of the head and neck (SCCHN) has emerged as a distinct clinical entity. The expression of p16 protein can be used as a surrogate for HPV status...
  33. pmc Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining
    Brandi L Mahaney
    Department of Biochemistry and Molecular Biology and The Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Biochem J 417:639-50. 2009
    ..In the present review, we will discuss our current understanding of the mechanism of NHEJ in mammalian cells and discuss the roles of DNA-PKcs and DNA-PK-mediated phosphorylation in NHEJ...
  34. doi request reprint PIK3CA mutational status and overall survival in patients with cervical cancer treated with radical chemoradiotherapy
    John B McIntyre
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary AB, Canada
    Gynecol Oncol 128:409-14. 2013
    ....
  35. pmc Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase
    Nicholas S Y Ting
    Department of Biochemistry and Molecular Biology, Cancer Biology Research Group, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Nucleic Acids Res 33:2090-8. 2005
    ....
  36. pmc Detection and repair of ionizing radiation-induced DNA double strand breaks: new developments in nonhomologous end joining
    Chen Wang
    Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Canada
    Int J Radiat Oncol Biol Phys 86:440-9. 2013
    ..Here we review recent advances on the mechanism of nonhomologous end joining, as well as new findings on its component proteins and regulation...
  37. pmc Nepenthesin from monkey cups for hydrogen/deuterium exchange mass spectrometry
    Martial Rey
    Department of Biochemistry and Molecular Biology and The Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Mol Cell Proteomics 12:464-72. 2013
    ..We conclude that nepenthesin is an effective alternative to pepsin for all HDX-MS applications, and especially for the analysis of structural transitions among intrinsically disordered proteins and their binding partners...
  38. pmc A structural model for regulation of NHEJ by DNA-PKcs autophosphorylation
    Tracey A Dobbs
    Department of Biochemistry, Southern Alberta Cancer Research Institute, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6, Canada
    DNA Repair (Amst) 9:1307-14. 2010
    ....
  39. ncbi request reprint Low ATM protein expression and depletion of p53 correlates with olaparib sensitivity in gastric cancer cell lines
    Eiji Kubota
    Department of Biochemistry and Molecular Biology University of Calgary Calgary, Alberta, Canada Southern Alberta Cancer Research Institute University of Calgary Calgary, Alberta, Canada
    Cell Cycle 13:2129-37. 2014
    ....
  40. ncbi request reprint UV-light induces p38 MAPK-dependent phosphorylation of Bcl10
    Amanda J Bodero
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, NW, AB T2N 4N1, Calgary, Canada
    Biochem Biophys Res Commun 301:923-6. 2003
    ..UV-induced phosphorylation of Bcl10 was inhibited by the p38 stress-activated protein kinase inhibitors SB203580 and PD169316, suggesting that p38 is required for UV-mediated phosphorylation of Bcl10...
  41. ncbi request reprint The C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining
    Brandi L Mahaney
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, Canada T2N 4N1
    DNA Repair (Amst) 14:9-16. 2014
    ....
  42. doi request reprint Low ERCC1 mRNA and protein expression are associated with worse survival in cervical cancer patients treated with radiation alone
    Corinne M Doll
    Department of Oncology, University of Calgary, Alberta, Canada
    Radiother Oncol 97:352-9. 2010
    ..To evaluate the association of excision repair cross-complementation group 1 (ERCC1) expression, using both mRNA and protein expression analysis, with clinical outcome in cervical cancer patients treated with radical radiation therapy (RT)...
  43. ncbi request reprint Structure and dynamics of lipoplex formation examined using two-photon fluorescence cross-correlation spectroscopy
    Dennis Merkle
    Department of Chemistry, University of Calgary, 2500 University Drive, NW, Calgary, Alberta T2N 1N4, Canada
    Biochemistry 43:7263-72. 2004
    ..The dependence of large aggregated lipoplex formation on DNA-to-cationic lipid charge ratio was determined, as was the maximum number of 40 bp double-stranded DNA oligonucleotides able to bind to a single vesicle...
  44. ncbi request reprint Intestinal infection with Giardia spp. reduces epithelial barrier function in a myosin light chain kinase-dependent fashion
    Kevin G E Scott
    Department of Biological Sciences, University of Calgary, Alberta, Canada
    Gastroenterology 123:1179-90. 2002
    ..This study was conducted to determine whether Giardia spp. alters intestinal permeability in vivo, to assess whether these abnormalities are dependent on T cells, and to assess the role of MLCK in altered epithelial barrier function...
  45. ncbi request reprint ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation
    Shin ichi Saito
    Biology Department, Brookhaven National Laboratory, 50 Bell Avenue, Upton, New York 11973, USA
    J Biol Chem 277:12491-4. 2002
    ..These observations, together with the proposed role of Ser(46) phosphorylation in mediating apoptosis, suggest that ATM is involved in the initiation of p53-dependent apoptosis after IR in human lymphoblastoid cells...
  46. pmc Autophosphorylation of DNA-dependent protein kinase regulates DNA end processing and may also alter double-strand break repair pathway choice
    Xiaoping Cui
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, 48824, USA
    Mol Cell Biol 25:10842-52. 2005
    ..We conclude that separate DNA-PK autophosphorylation events may function reciprocally by not only regulating DNA end processing but also affecting DSB repair pathway choice...
  47. ncbi request reprint DNA end sequestration by DNA-dependent protein kinase and end joining of sterically constrained substrates in whole-cell extracts
    Jae Wan Lee
    Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, Virginia 23298 0230, USA
    Environ Mol Mutagen 42:279-87. 2003
    ..In addition, end joining in human extracts appears to involve interactions with significantly longer segments of DNA than the approximately 28 bp occupied by DNA-PK...
  48. pmc Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair
    Qi Ding
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
    Mol Cell Biol 23:5836-48. 2003
    ..However, autophosphorylation at these sites may not be sufficient for kinase disassembly...
  49. pmc Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366
    Gopal P Sapkota
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:507-16. 2002
    ..These observations provide the first link between ATM and LKB1 and suggest that ATM could regulate LKB1...
  50. ncbi request reprint Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule
    Dennis J Sawchuk
    Laboratory of Molecular Immunology and Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 279:29821-31. 2004
    ....
  51. pmc Phosphorylation in the serine/threonine 2609-2647 cluster promotes but is not essential for DNA-dependent protein kinase-mediated nonhomologous end joining in human whole-cell extracts
    Lawrence F Povirk
    Department of Pharmacology and Toxicology, Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
    Nucleic Acids Res 35:3869-78. 2007
    ....
  52. ncbi request reprint The DNA-dependent protein kinase: the director at the end
    Katheryn Meek
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA
    Immunol Rev 200:132-41. 2004
    ..In this review, we discuss recent developments that have provided insight into how DNA-PK functions, once bound to DNA ends...
  53. ncbi request reprint The double (strand break) life of Par-3
    Susan P Lees-Miller
    Nat Cell Biol 9:363-5. 2007
  54. pmc trans Autophosphorylation at DNA-dependent protein kinase's two major autophosphorylation site clusters facilitates end processing but not end joining
    Katheryn Meek
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA
    Mol Cell Biol 27:3881-90. 2007
    ..These data support an emerging consensus that, via a series of autophosphorylation events, DNA-PK undergoes a sequence of conformational changes that promote efficient and appropriate repair of DSBs...
  55. pmc DNA-PK autophosphorylation facilitates Artemis endonuclease activity
    Aaron A Goodarzi
    Genome Damage and Stability Centre, University of Sussex, East Sussex, UK
    EMBO J 25:3880-9. 2006
    ..These findings demonstrate that DNA-PK autophosphorylation regulates Artemis access to DNA ends, providing insight into the mechanism of Artemis mediated DNA end processing...
  56. ncbi request reprint Deficiency in the catalytic subunit of DNA-dependent protein kinase causes down-regulation of ATM
    Yuanlin Peng
    Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    Cancer Res 65:1670-7. 2005
    ....
  57. pmc Inhibition of homologous recombination by variants of the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs)
    Erin Convery
    Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 102:1345-50. 2005
    ....
  58. ncbi request reprint Non-homologous end joining requires that the DNA-PK complex undergo an autophosphorylation-dependent rearrangement at DNA ends
    Yeturu V R Reddy
    Lineberger Comprehensive Cancer Center and Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 279:39408-13. 2004
    ..Autophosphorylation at ABCDE sites thus apparently directs a rearrangement of the DNA-PK complex that ensures access to broken ends and joining steps are coupled together within a synaptic complex, making repair more accurate...
  59. ncbi request reprint Putative homologues of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and other components of the non-homologous end joining machinery in Dictyostelium discoideum
    Wesley D Block
    DNA Repair (Amst) 4:1061-5. 2005
  60. ncbi request reprint Conversion of phosphoglycolate to phosphate termini on 3' overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1
    Kedar V Inamdar
    Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA
    J Biol Chem 277:27162-8. 2002
    ..The results suggest a role for hTdp1 in repair of free radical-mediated DNA double strand breaks bearing terminally blocked 3' overhangs...
  61. pmc Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA
    Elena Bochkareva
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    EMBO J 21:1855-63. 2002
    ..Taken together, our data indicate that switching from 8-10 to 30 nt mode is mediated by DNA binding with the trimerization core...