J R Lakey

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. ncbi request reprint Long-term graft function after allogeneic islet transplantation
    Jonathan R T Lakey
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 16:441-6. 2007
  2. ncbi request reprint Supply of human allograft tissue in Canada
    Jonathan R T Lakey
    Clinical Islet Transplant Program, Department of Surgery, University of Alberta, 8215 112 Street, Suite 210, College Plaza, T6G 2C8 Edmonton, Alberta, Canada
    Cell Tissue Bank 8:135-50. 2007
  3. ncbi request reprint Technical aspects of islet preparation and transplantation
    Jonathan R T Lakey
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, T6G 2N8, Edmonton, Alberta, Canada
    Transpl Int 16:613-32. 2003
  4. ncbi request reprint Dynamics of cryoprotectant permeation in porcine heart valve leaflets
    Jonathan R T Lakey
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Cell Transplant 12:123-8. 2003
  5. ncbi request reprint Preservation of the human pancreas before islet isolation using a two-layer (UW solution-perfluorochemical) cold storage method
    Jonathan R T Lakey
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1809-11. 2002
  6. ncbi request reprint Demand for human allograft tissue in Canada
    Jonathan R T Lakey
    Clinical Islet Transplant Program, Department of Surgery, Faculty of Medicine, University of Alberta, 8215 112 Street, Suite 210, College Plaza, T6G 2C8 Edmonton, AB, Canada
    Cell Tissue Bank 8:31-42. 2007
  7. ncbi request reprint Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability
    Jonathan R T Lakey
    Department of Surgery and the Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta T6G 2N8, Canada
    Transplantation 73:1106-10. 2002
  8. ncbi request reprint Islet cryopreservation using intracellular preservation solutions
    J R Lakey
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Canada
    Cell Transplant 10:583-9. 2001
  9. ncbi request reprint Novel approaches to cryopreservation of human pancreatic islets
    J R Lakey
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, T6G 2N8 Canada
    Transplantation 72:1005-11. 2001
  10. ncbi request reprint Secretion from islets and single islet cells following cryopreservation
    J R Lakey
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Cell Transplant 8:691-8. 1999

Collaborators

Detail Information

Publications74

  1. ncbi request reprint Long-term graft function after allogeneic islet transplantation
    Jonathan R T Lakey
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 16:441-6. 2007
    ..In contrast, sustained insulin secretion with nearly normal HbA1c at 13 years follow-up was observed in another patient, providing hope for improving long-term graft outcomes for islet transplant recipient...
  2. ncbi request reprint Supply of human allograft tissue in Canada
    Jonathan R T Lakey
    Clinical Islet Transplant Program, Department of Surgery, University of Alberta, 8215 112 Street, Suite 210, College Plaza, T6G 2C8 Edmonton, Alberta, Canada
    Cell Tissue Bank 8:135-50. 2007
    ..Through estimating Current supply, and compiling additional qualitative information, this study has provided a snapshot of the current Canadian supply and shortfall of allograft tissue grafts...
  3. ncbi request reprint Technical aspects of islet preparation and transplantation
    Jonathan R T Lakey
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, T6G 2N8, Edmonton, Alberta, Canada
    Transpl Int 16:613-32. 2003
    ..This chapter will review the rationale of islet transplantation and the development of islet isolation and purification. The challenges facing clinical islet transplantation in the twenty-first century will also be introduced...
  4. ncbi request reprint Dynamics of cryoprotectant permeation in porcine heart valve leaflets
    Jonathan R T Lakey
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Cell Transplant 12:123-8. 2003
    ..The clinical applications of these findings may help to optimize the balance between the protective and toxic effects of cryoprotectants and lead to improved methods of preservation of heart valves...
  5. ncbi request reprint Preservation of the human pancreas before islet isolation using a two-layer (UW solution-perfluorochemical) cold storage method
    Jonathan R T Lakey
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1809-11. 2002
  6. ncbi request reprint Demand for human allograft tissue in Canada
    Jonathan R T Lakey
    Clinical Islet Transplant Program, Department of Surgery, Faculty of Medicine, University of Alberta, 8215 112 Street, Suite 210, College Plaza, T6G 2C8 Edmonton, AB, Canada
    Cell Tissue Bank 8:31-42. 2007
    ..The highest rated preferences (98% and 94%) were for accredited and Canadian tissue banks, respectively. This study represents a key step in addressing the paucity of information concerning the demand for allograft tissue in Canada...
  7. ncbi request reprint Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability
    Jonathan R T Lakey
    Department of Surgery and the Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta T6G 2N8, Canada
    Transplantation 73:1106-10. 2002
    ..The aim of this study was to correlate pancreas procurement technique with the core temperature within the pancreas during in situ procurement to the recovery and in vitro function of isolated human islets...
  8. ncbi request reprint Islet cryopreservation using intracellular preservation solutions
    J R Lakey
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Canada
    Cell Transplant 10:583-9. 2001
    ..6 +/- 0.5, and 0.4 +/- 0.2 for islets in groups 1-5, respectively. This study demonstrates that postcryopreservation survival can be improved using intracellular-based preservation solutions, including UW or HTS, in conjunction with DMSO...
  9. ncbi request reprint Novel approaches to cryopreservation of human pancreatic islets
    J R Lakey
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, T6G 2N8 Canada
    Transplantation 72:1005-11. 2001
    ..Furthermore, the effect of different addition protocols and equilibrium concentrations of the cryoprotectants were studied...
  10. ncbi request reprint Secretion from islets and single islet cells following cryopreservation
    J R Lakey
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Cell Transplant 8:691-8. 1999
    ..It was determined that beta-cells recovered following cryopreservation are capable of secreting insulin at levels and frequencies comparable to those of cultured nonfrozen islet preparations...
  11. ncbi request reprint Nonviral transfection of intact pancreatic islets
    J R Lakey
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Cell Transplant 10:697-708. 2001
    ..5 and 3.4+/-0.5, respectively, p < 0.05). Lipid-based transfection using DOTAP at a DNA/lipid ratio of 1:6 provides an effective means of ex vivo gene delivery without compromising in vitro functionality of the transfected islets...
  12. ncbi request reprint Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen
    A M Shapiro
    Surgical Medical Research Institute and the Department of Surgery, University of Alberta, Edmonton, Canada
    N Engl J Med 343:230-8. 2000
    ..Registry data on patients with type 1 diabetes mellitus who undergo pancreatic islet transplantation indicate that only 8 percent are free of the need for insulin therapy at one year...
  13. ncbi request reprint Osmotic and cryoprotectant permeation characteristics of islet cells isolated from the newborn pig pancreas
    C Fedorow
    Department of Surgery, University of Alberta, Edmonton, Canada
    Cell Transplant 10:651-9. 2001
    ....
  14. ncbi request reprint Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol
    E A Ryan
    Department of Medicine, Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Diabetes 50:710-9. 2001
    ..An elevation of creatinine appears to be a contraindication to this immunosuppressive regimen. For the subjects who had labile type 1 diabetes that was difficult to control, the risk-to-benefit ratio is in favor of islet transplantation...
  15. ncbi request reprint Novel approaches toward early diagnosis of islet allograft rejection
    A M Shapiro
    Department of Surgery, University of Alberta Hospitals, Mackenzie Health Sciences Center, 8440 112 Street, Edmonton, AB, Canada T6G 2B7
    Transplantation 71:1709-18. 2001
    ..We explored three novel approaches toward development of early diagnostic markers of islet rejection after islet allotransplantation...
  16. ncbi request reprint Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia
    J R Lakey
    Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Canada
    Transplantation 72:565-70. 2001
    ..CONCLUSIONS: Inhibition of intrinsic protease activity within pancreases after prolonged cold storage improves isolation of viable islets...
  17. ncbi request reprint Pancreatic islet transplantation in the treatment of diabetes mellitus
    A M Shapiro
    Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton, AB, Canada
    Best Pract Res Clin Endocrinol Metab 15:241-64. 2001
    ..Recent advances in stem cell biology and xenotransplantation may soon provide this opportunity...
  18. ncbi request reprint Peroxynitrite is a mediator of cytokine-induced destruction of human pancreatic islet beta cells
    J R Lakey
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Lab Invest 81:1683-92. 2001
    ....
  19. ncbi request reprint Decellularization reduces the immune response to aortic valve allografts in the rat
    Steven R Meyer
    Department of Surgery, University of Alberta, Edmonston, Canada
    J Thorac Cardiovasc Surg 130:469-76. 2005
    ..This might be reduced by removal of antigenic cellular elements (decellularization). The aim of this study was to determine the immunologic effect of decellularization in a rat allograft valve model...
  20. ncbi request reprint Ameliorating injury during preservation and isolation of human islets using the two-layer method with perfluorocarbon and UW solution
    Payam Salehi
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Cell Transplant 15:187-94. 2006
    ....
  21. ncbi request reprint Estimation of pancreas weight from donor variables
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 15:181-5. 2006
    ..These formulae should contribute to the estimation of pancreas weight, and thus improve donor selection for islet isolation and transplantation...
  22. ncbi request reprint Comparison of cooling systems during islet purification
    Sue Swift
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 15:175-80. 2006
    ..7 degrees C, p < 0.05). The original cooler system was found to be more efficient at reducing heat generated by the COBE machine than the air system. Further investigation of the importance of the recorded temperatures is required...
  23. ncbi request reprint Oxygen and pancreas preservation
    Mohammadreza Mirbolooki
    Transplantation 81:492; author reply 492-3. 2006
  24. ncbi request reprint Strategic opportunities in clinical islet transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Canada
    Transplantation 79:1304-7. 2005
    ..Future opportunities to expand the donor pool using living donor islet transplantation are within reach, and will be enhanced considerably with both donor and recipient adjunctive treatment using islet-specific growth-factors...
  25. ncbi request reprint Quantitative assessment of collagenase blends for human islet isolation
    Matthew J Barnett
    Human Islet Investigations Laboratory, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 80:723-8. 2005
    ..Examination of the formulation and potency of the widely used Liberase HI enzyme blend will uncover possible sources of imprecision...
  26. ncbi request reprint A human beta-cell line for transplantation therapy to control type 1 diabetes
    Michiki Narushima
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Biotechnol 23:1274-82. 2005
    ..The establishment of this cell line is one step toward a potential cure of diabetes by transplantation...
  27. ncbi request reprint Five-year follow-up after clinical islet transplantation
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, 2000 College Plaza, 8215 112th St, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 54:2060-9. 2005
    ..The results, though promising, still point to the need for further progress in the availability of transplantable islets, improving islet engraftment, preserving islet function, and reducing toxic immunosuppression...
  28. ncbi request reprint XIAP overexpression in human islets prevents early posttransplant apoptosis and reduces the islet mass needed to treat diabetes
    Juliet A Emamaullee
    1 21 Medical Sciences Building, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    Diabetes 54:2541-8. 2005
    ..These results suggest that by transiently overexpressing XIAP in the immediate posttransplant period, human islets from a single donor might be used to effectively treat two diabetic recipients...
  29. ncbi request reprint Assessment of glycemic control after islet transplantation using the continuous glucose monitor in insulin-independent versus insulin-requiring type 1 diabetes subjects
    Breay W Paty
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Diabetes Technol Ther 8:165-73. 2006
    ....
  30. ncbi request reprint Stem cell sources for clinical islet transplantation in type 1 diabetes: embryonic and adult stem cells
    Helena Miszta-Lane
    Clinical Islet Transplantation Program, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, 1074 Dentistry Pharmacy Centre, Edmonton, Alta, Canada T6G 2N8
    Med Hypotheses 67:909-13. 2006
    ..In this study, we will review the different aspects and challenges of using embryonic or adult stem cells in clinical islet transplantation for the treatment of type 1 diabetes...
  31. ncbi request reprint Current status of clinical islet cell transplantation
    Jonathan R T Lakey
    Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    Methods Mol Biol 333:47-104. 2006
    ..The challenges facing the clinician-scientist in the 21st century are also presented in this review...
  32. ncbi request reprint Combination therapy with glucagon-like peptide-1 and gastrin induces beta-cell neogenesis from pancreatic duct cells in human islets transplanted in immunodeficient diabetic mice
    Wilma L Suarez-Pinzon
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Cell Transplant 17:631-40. 2008
    ....
  33. ncbi request reprint Detection of microbial contamination during human islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 16:9-13. 2007
    ..Microbial contamination in final products is rare, but de novo contamination still occurs during processing even under cGMP conditions...
  34. ncbi request reprint Factors influencing the collagenase digestion phase of human islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Canada
    Transplantation 83:7-12. 2007
    ..Such innovative strategies will be advantageous in improving islet isolation efficiency, recovery, viability, and ultimately posttransplant function...
  35. ncbi request reprint Compaction of islets is detrimental to transplant outcome in mice
    Shaheed Merani
    Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton AB, Canada
    Transplantation 82:1472-6. 2006
    ..We herein investigate the potential detrimental impact of maintaining islets in a pelleted state for periods preceding implantation. We hypothesize that periods of islet compaction lead to impairment if islet function in vivo...
  36. ncbi request reprint Islet isolation and transplantation outcomes of pancreas preserved with University of Wisconsin solution versus two-layer method using preoxygenated perfluorocarbon
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health, Edmonton, Alberta, Canada
    Transplantation 82:1286-90. 2006
    ..However, the effect of TLM has not been evaluated in large-scale study. In addition, a direct benefit of TLM on islet transplantation outcome has not been addressed in the setting of any randomized controlled trials...
  37. ncbi request reprint International trial of the Edmonton protocol for islet transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    N Engl J Med 355:1318-30. 2006
    ..We conducted an international, multicenter trial to explore the feasibility and reproducibility of islet transplantation with the use of a single common protocol (the Edmonton protocol)...
  38. ncbi request reprint Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts
    Atsushi Miki
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cell Transplant 15:325-34. 2006
    ..A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system...
  39. ncbi request reprint Comparison of aortic valve allograft decellularization techniques in the rat
    Steven R Meyer
    Department of Surgery, University of Alberta, Edmonton, AB, Canada
    J Biomed Mater Res A 79:254-62. 2006
    ..Osmotic lysis protocols with low dose detergent appear to be most effective at both removing antigenic cellular elements and preserving ECM...
  40. ncbi request reprint Enhancing the success of human islet isolation through optimization and characterization of pancreas dissociation enzyme
    T Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health, Canada
    Am J Transplant 7:1233-41. 2007
    ..204 was used, suggesting that CII/CI ratio may be a strong predictor to distinguish potential lot success. This study provides a framework for improved enzymatic digestion in human islet isolation...
  41. ncbi request reprint Islet isolation and transplantation from an annular pancreas: a case report
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    JOP 6:274-6. 2005
    ..Annular pancreas is an uncommon congenital anomaly formed by a thin band of normal pancreatic tissue encircling the duodenum...
  42. ncbi request reprint Pancreas divisum: a study of the cadaveric donor pancreas for islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Pancreas 30:325-7. 2005
    ..We investigated the frequency of pancreas divisum and its effect on the digestion process in islet isolation...
  43. ncbi request reprint Prevalence of hepatic steatosis after islet transplantation and its relation to graft function
    Ravi Bhargava
    Department of Radiology and Diagnostic Imaging, 2A2 42 Walter C Mackenzie Health Sciences Centre, University of Alberta, 8440 112 Street, Edmonton, Alberta T6G 2B7, Canada
    Diabetes 53:1311-7. 2004
    ..001). MRI-detected steatosis is a common finding; the steatosis appears to be due to a paracrine action of insulin secreted from intrahepatic islets. Hepatic steatosis may be associated with insulin resistance or graft dysfunction...
  44. ncbi request reprint Alleviating ischemia-reperfusion injury in small bowel
    Payam Salehi
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Building, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Am J Transplant 4:728-37. 2004
    ..This study raises the possibility of a role for a luminal nutrient-rich solution during ischemic storage of small bowel in the clinic...
  45. ncbi request reprint Islet auto-transplantation into an omental or splenic site results in a normal beta cell but abnormal alpha cell response to mild non-insulin-induced hypoglycemia
    Stephanie M Gustavson
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA
    Am J Transplant 5:2368-77. 2005
    ..In conclusion, in conscious dogs with successfully auto-transplanted islets, the beta cell response to mild non-insulin-induced hypoglycemia was normal, whereas the alpha cell response was not...
  46. ncbi request reprint Short-term storage of the ischemically damaged human pancreas by the two-layer method prior to islet isolation
    Toshiaki Tsujimura
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta T6G2N8, Canada
    Cell Transplant 13:67-73. 2004
    ..Use of the TLM following prolonged storage in UW provides an excellent adjunctive protocol for treating human pancreata for the rigors of the islet isolation process...
  47. ncbi request reprint Changes in liver enzymes after clinical islet transplantation
    Ehab Rafael
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 76:1280-4. 2003
    ..The aim of this study was to characterize the pattern of elevation in liver enzymes observed after ITx and to investigate any correlation between these elevations and graft characteristics or graft functional outcome...
  48. ncbi request reprint Percutaneous transhepatic pancreatic islet cell transplantation in type 1 diabetes mellitus: radiologic aspects
    Richard J T Owen
    Departments of Diagnostic Imaging, University of Alberta Hospital, 8440 112th Street, Edmonton, Alberta, Canada T6G 2B7
    Radiology 229:165-70. 2003
    ..To report our experience with percutaneous transhepatic pancreatic islet cell transplantation in patients with type 1 diabetes mellitus...
  49. ncbi request reprint An evaluation of endogenous pancreatic enzyme levels after human islet isolation
    Natisha L Rose
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Pancreas 27:167-73. 2003
    ..Following the digestion, it is important to further inhibit these enzymes by the addition of an inhibitor to the dilution media...
  50. ncbi request reprint A novel technique of hypothermic luminal perfusion for small bowel preservation
    Jay Z J Zhu
    Surgical Medical Institute, University of Alberta, Edmonton, Canada
    Transplantation 76:71-6. 2003
    ..This study improved small bowel preservation using University of Wisconsin (UW) solution in conjunction with hypothermic luminal perfusion...
  51. ncbi request reprint Evaluation of Pefabloc as a serine protease inhibitor during human-islet isolation
    Natisha L Rose
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 75:462-6. 2003
    ..In this study, we have hypothesized that this improvement was because of the inhibition of three key serine proteases...
  52. ncbi request reprint Evaluating the effect of serine proteases on collagenase activity during human islet isolation
    Natisha L Rose
    Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada
    Cell Transplant 11:821-6. 2002
    ..Further efforts at examining exogenous and endogenous enzyme levels may result in the development of an enzyme cocktail that is both stable and effective for digesting the human pancreas while preserving islet function and viability...
  53. ncbi request reprint Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW solution/perfluorochemical) cold-storage method
    Toshiaki Tsujimura
    Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1687-91. 2002
    ..In this study, we determined whether the additional preservation by the two-layer method could improve islet recovery from human pancreases with prolonged cold storage in UW...
  54. ncbi request reprint Defining optimal immunosuppression for islet transplantation based on reduced diabetogenicity in canine islet autografts
    A M James Shapiro
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1522-8. 2002
    ..We here explore the effects of clinically relevant doses of cyclosporine or tacrolimus when given alone or in combination with glucocorticoids on long-term canine islet autograft function...
  55. ncbi request reprint Intrahepatic islet transplantation in type 1 diabetic patients does not restore hypoglycemic hormonal counterregulation or symptom recognition after insulin independence
    Breay W Paty
    Clinical Islet Transplant Program, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 51:3428-34. 2002
    ....
  56. ncbi request reprint Endogenous pancreatic enzyme activity levels show no significant effect on human islet isolation yield
    Natisha L Rose
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, T6G 2N8, Canada
    Cell Transplant 13:153-60. 2004
    ..This suggests that further understanding of the isolation process should focus on the role of the collagenase digestion solution in the dissociation of the endocrine-exocrine tissue connection...
  57. ncbi request reprint Maintenance of glucose-sensitive insulin secretion of cryopreserved human islets with University of Wisconsin solution and ascorbic acid-2 glucoside
    Takashi Arata
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Artif Organs 28:529-36. 2004
    ..Human islets monolayer-cultured with 804G-derived ECM are efficiently transduced with a lentiviral vector Lt-NLS/LacZ...
  58. ncbi request reprint Use of an allograft patch in repair of hypoplastic left heart syndrome may complicate future transplantation
    Steven R Meyer
    Department of Surgery, University of Alberta Hospital and Stollery Children s Hospital, Edmonton, Alta, Canada
    Eur J Cardiothorac Surg 27:554-60. 2005
    ..The purpose of this study was to assess the anti-HLA antibody response to allograft patches used in the initial repair of HLHS...
  59. ncbi request reprint Beta-score: an assessment of beta-cell function after islet transplantation
    Edmond A Ryan
    Heritage Medical Research Centre, Edmonton, Alberta, Canada T6G 2S2
    Diabetes Care 28:343-7. 2005
    ..For the current study, a composite beta-score was developed that provides an integrated measure of beta-cell function success after islet transplantation...
  60. ncbi request reprint Islet graft assessment in the Edmonton Protocol: implications for predicting long-term clinical outcome
    Cale N Street
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Diabetes 53:3107-14. 2004
    ..In summary, careful assessment of islet graft composition is needed in a clinical transplantation program to accurately estimate islet purity and assess the contribution of other cell types present, such as islet progenitor cells...
  61. ncbi request reprint Successful islet transplantation: continued insulin reserve provides long-term glycemic control
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, 2000 College Plaza, 8215 112th Street, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 51:2148-57. 2002
    ..Islet transplantation is a reasonable option in those with severe problems with glycemic lability or hypoglycemia...
  62. ncbi request reprint Cross-sectional and prospective association between proinsulin secretion and graft function after clinical islet transplantation
    Charlotte G McDonald
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 78:934-7. 2004
    ..TP and PF levels were reduced in transplant recipients compared with controls but increased over time if insulin independence was lost...
  63. ncbi request reprint Influence of pancreas preservation on human islet isolation outcomes: impact of the two-layer method
    Toshiaki Tsujimura
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Transplantation 78:96-100. 2004
    ..The purpose of this study was to clarify the limitations of the University of Wisconsin (UW) solution and the potentials of the two-layer method (TLM) for pancreas preservation before human islet isolation...
  64. ncbi request reprint Clinical islet transplant: current and future directions towards tolerance
    A M James Shapiro
    Clinical Islet Transplant Program and Department of Surgery, University of Alberta, 200 College Plaza, 8215 112 Street, Edmonton, Alberta, Canada T6G 2C8
    Immunol Rev 196:219-36. 2003
    ....
  65. ncbi request reprint Pancreatic islet autotransplantation with completion pancreatectomy in the management of uncontrolled pancreatic fistula after whipple resection for ampullary adenocarcinoma
    Faisal Alsaif
    Pancreas 32:430-1. 2006
  66. ncbi request reprint A novel approach to increase human islet cell mass while preserving beta-cell function
    Gillian M Beattie
    Department of Pediatrics and Surgery, The Whittier Institute, University of California at San Diego, 9894 Genesee Avenue, La Jolla, CA 92037, USA
    Diabetes 51:3435-9. 2002
    ..Limited islet ex vivo expansion under these conditions may improve recipient-donor tissue ratios to equal the functional results of whole-organ transplants...
  67. ncbi request reprint Combination therapy with epidermal growth factor and gastrin induces neogenesis of human islet {beta}-cells from pancreatic duct cells and an increase in functional {beta}-cell mass
    Wilma L Suarez-Pinzon
    430 Heritage Medical Research Center, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    J Clin Endocrinol Metab 90:3401-9. 2005
    ....
  68. ncbi request reprint Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas
    Ergeng Hao
    Rebecca and John Moores UCSD Cancer Center, University of California San Diego, 9500 Gilman Drive, MC 0816, La Jolla, California 92093, USA
    Nat Med 12:310-6. 2006
    ..Nonendocrine-to-endocrine differentiation of NEPECs supports the existence of endocrine stem or progenitor cells within the epithelial compartment of the adult human pancreas...
  69. ncbi request reprint The portal immunosuppressive storm: relevance to islet transplantation?
    A M James Shapiro
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Ther Drug Monit 27:35-7. 2005
    ..This "portal storm" of immunosuppression may be relevant to intrahepatic islet transplantation...
  70. ncbi request reprint The current situation in human pancreatic islet transplantation: problems and prospects
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
    J Artif Organs 7:1-8. 2004
    ..Here we present general information on islet transplantation and discuss its problems and prospects...
  71. ncbi request reprint Expansion of mesenchymal stem cells from human pancreatic ductal epithelium
    Karen L Seeberger
    Surgical Medical Research Institute, University of Alberta, Edmonton, AB, Canada
    Lab Invest 86:141-53. 2006
    ..Therefore, multipotent MSCs residing within the adult exocrine pancreas could represent a progenitor cell, which when further manipulated could result in the production of functional islet beta-cells...
  72. ncbi request reprint Sirolimus-induced ulceration of the small bowel in islet transplant recipients: report of two cases
    Michele Molinari
    Department of Surgery, Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Am J Transplant 5:2799-804. 2005
    ..Our experience highlights an uncommon but potentially serious adverse effect of high-dose SRL in islet recipients...
  73. ncbi request reprint The standardization of pancreatic donors for islet isolations
    Doug O'Gorman
    Clinical Islet Transplant Program, Department of Transplant Services, University of Alberta Hospital, Edmonton, Alberta, Canada
    Transplantation 80:801-6. 2005
    ..The aim of this study was to establish an algorithm by which the combination of the donor quality and pancreas quality was given a numerical score from 0 to 100 for use in determining the quality of a pancreas for islet isolation...
  74. ncbi request reprint Development of a novel beta-cell specific promoter system for the identification of insulin-producing cells in in vitro cell cultures
    Takuya Fukazawa
    First Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho 2 5 1, Okayama 700 8558, Japan
    Exp Cell Res 312:3404-12. 2006
    ..Here, we describe a novel beta-cell specific and glucose responsive artificial promoter system designed for analyzing and sorting beta-like insulin-producing cells that have differentiated from stem cells or other progenitor cells...