John S Klassen


Affiliation: University of Alberta
Country: Canada


  1. Kitov P, Han L, Kitova E, Klassen J. Sliding Window Adduct Removal Method (SWARM) for Enhanced Electrospray Ionization Mass Spectrometry Binding Data. J Am Soc Mass Spectrom. 2019;: pubmed publisher
    ..Graphical Abstract. ..
  2. Li J, Han L, Li J, Kitova E, Xiong Z, Prive G, et al. Detecting Protein-Glycolipid Interactions Using CaR-ESI-MS and Model Membranes: Comparison of Pre-loaded and Passively Loaded Picodiscs. J Am Soc Mass Spectrom. 2018;29:1493-1504 pubmed publisher
    ..Together, the results of this study suggest PLPDs may have advantages over conventionally prepared PDs for screening glycolipids against GBPs using CaR-ESI-MS. Graphical Abstract ᅟ. ..
  3. Han L, Kitova E, Tan M, Jiang X, Pluvinage B, Boraston A, et al. Affinities of human histo-blood group antigens for norovirus capsid protein complexes. Glycobiology. 2015;25:170-80 pubmed publisher
  4. El Hawiet A, Kitova E, Klassen J. Recognition of human milk oligosaccharides by bacterial exotoxins. Glycobiology. 2015;25:845-54 pubmed publisher
    ..Taken together, the results of this study suggest that, while HMOs are expected to bind extensively to these bacterial toxins, it is unlikely that HMO binding will effectively inhibit their interactions with their cellular receptors. ..
  5. Yao Y, Richards M, Kitova E, Klassen J. Influence of Sulfolane on ESI-MS Measurements of Protein-Ligand Affinities. J Am Soc Mass Spectrom. 2016;27:498-506 pubmed publisher
    ..Binding measurements performed using liquid sample desorption ESI-MS revealed that protein supercharging with sulfolane can be achieved without a reduction in affinity. ..
  6. request reprint
    Han L, Kitova E, Klassen J. Detecting Protein-Glycolipid Interactions Using Glycomicelles and CaR-ESI-MS. J Am Soc Mass Spectrom. 2016;27:1878-1886 pubmed
    ..Graphical Abstract <!-- [INSERT GRAPHICAL ABSTRACT TEXT HERE] -->. ..
  7. Zhang J, Kitova E, Li J, Eugenio L, Ng K, Klassen J. Localizing Carbohydrate Binding Sites in Proteins Using Hydrogen/Deuterium Exchange Mass Spectrometry. J Am Soc Mass Spectrom. 2016;27:83-90 pubmed publisher
    ..However, a detailed interpretation of the changes in deuterium exchange upon ligand binding can be challenging because of the presence of ligand-induced changes in protein structure and dynamics. ..
  8. Rezaei Darestani R, Winter P, Kitova E, Tuszynski J, Klassen J. Screening Anti-Cancer Drugs against Tubulin using Catch-and-Release Electrospray Ionization Mass Spectrometry. J Am Soc Mass Spectrom. 2016;27:876-85 pubmed publisher
    ..The assay was then used to screen a library of seven colchicinoid analogues to test their binding to tubulin and to rank their affinities. ..
  9. Liu L, Siuda I, Richards M, Renaud J, Kitova E, Mayer P, et al. Structure and Stability of Carbohydrate-Lipid Interactions. Methylmannose Polysaccharide-Fatty Acid Complexes. Chembiochem. 2016;17:1571-8 pubmed publisher
    ..The MD results point to hydrophobic interactions and H-bonds with the FA carboxyl group. Comparison of collision cross sections of deprotonated (MMP+FA) ions with MD structures suggests that the gaseous complexes are disordered. ..

More Information


  1. El Hawiet A, Chen Y, Shams Ud Doha K, Kitova E, Kitov P, Bode L, et al. Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS. Analyst. 2018;143:536-548 pubmed publisher
    ..The affinities of eight purified HMOs for BabA were measured by ESI-MS and found to be in the 103 M-1 to 104 M-1 range. ..
  2. Li J, Richards M, Kitova E, Klassen J. Delivering Transmembrane Peptide Complexes to the Gas Phase Using Nanodiscs and Electrospray Ionization. J Am Soc Mass Spectrom. 2017;28:2054-2065 pubmed publisher
    ..These findings highlight the potential of NDs, combined with ESI, for transferring transmembrane peptide complexes directly from lipid bilayers to the gas phase. Graphical Abstract ᅟ. ..