James D Johnson

Summary

Affiliation: University of British Columbia
Country: Canada

Publications

  1. pmc A multi-parameter, high-content, high-throughput screening platform to identify natural compounds that modulate insulin and Pdx1 expression
    Jessica A Hill
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 5:e12958. 2010
  2. pmc Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome
    James D Johnson
    Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Proc Natl Acad Sci U S A 103:19575-80. 2006
  3. ncbi request reprint Control of pancreatic beta-cell fate by insulin signaling: The sweet spot hypothesis
    James D Johnson
    Laboratory of Molecular Signalling in Diabetes, Life Sciences Institute, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Cell Cycle 7:1343-7. 2008
  4. pmc Impaired insulin secretion in transgenic mice over-expressing calpastatin in pancreatic β-cells
    James D Johnson
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, Canada
    Islets 1:242-8. 2009
  5. ncbi request reprint Suppressed insulin signaling and increased apoptosis in CD38-null islets
    James D Johnson
    Division of Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Diabetes 55:2737-46. 2006
  6. ncbi request reprint Proteomic identification of carboxypeptidase E connects lipid-induced beta-cell apoptosis and dysfunction in type 2 diabetes
    James D Johnson
    Laboratory of Molecular Signalling in Diabetes, Life Sciences Institute, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Cell Cycle 8:38-42. 2009
  7. ncbi request reprint Different effects of FK506, rapamycin, and mycophenolate mofetil on glucose-stimulated insulin release and apoptosis in human islets
    James D Johnson
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Cell Transplant 18:833-45. 2009
  8. doi request reprint Mechanisms of pancreatic beta-cell apoptosis in diabetes and its therapies
    James D Johnson
    Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3
    Adv Exp Med Biol 654:447-62. 2010
  9. pmc Roles of IP3R and RyR Ca2+ channels in endoplasmic reticulum stress and beta-cell death
    Dan S Luciani
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Comlumbia, Canada
    Diabetes 58:422-32. 2009
  10. ncbi request reprint RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets
    James D Johnson
    Department of Internal Medicine, Washington University, St Louis, Missouri 63110, USA
    J Biol Chem 279:24794-802. 2004

Collaborators

Detail Information

Publications57

  1. pmc A multi-parameter, high-content, high-throughput screening platform to identify natural compounds that modulate insulin and Pdx1 expression
    Jessica A Hill
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 5:e12958. 2010
    ..This work represents an important step towards the development of drugs to increase insulin expression in diabetes and during in vitro differentiation of beta-cell replacements...
  2. pmc Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome
    James D Johnson
    Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Proc Natl Acad Sci U S A 103:19575-80. 2006
    ..Bridge-1, a Pdx1-binding partner and regulator of beta-cell survival, was increased significantly at low insulin doses. Together, these data suggest that insulin can act as a master regulator of islet survival by regulating Pdx1...
  3. ncbi request reprint Control of pancreatic beta-cell fate by insulin signaling: The sweet spot hypothesis
    James D Johnson
    Laboratory of Molecular Signalling in Diabetes, Life Sciences Institute, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Cell Cycle 7:1343-7. 2008
    ..We also highlight the key experiments that are required to further test this hypothesis...
  4. pmc Impaired insulin secretion in transgenic mice over-expressing calpastatin in pancreatic β-cells
    James D Johnson
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, Canada
    Islets 1:242-8. 2009
    ..These studies provide the first evidence that blocking endogenous calpain activity partially impairs insulin release in vivo and in vitro by targeting distal components of the insulin exocytotic machinery...
  5. ncbi request reprint Suppressed insulin signaling and increased apoptosis in CD38-null islets
    James D Johnson
    Division of Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Diabetes 55:2737-46. 2006
    ..Thus, our results suggest that CD38 plays a role in novel antiapoptotic signaling pathways but does not directly control glucose signaling in pancreatic beta-cells...
  6. ncbi request reprint Proteomic identification of carboxypeptidase E connects lipid-induced beta-cell apoptosis and dysfunction in type 2 diabetes
    James D Johnson
    Laboratory of Molecular Signalling in Diabetes, Life Sciences Institute, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Cell Cycle 8:38-42. 2009
    ..These newly identified targets of beta-cell lipotoxicity present novel avenues for research and therapeutic intervention...
  7. ncbi request reprint Different effects of FK506, rapamycin, and mycophenolate mofetil on glucose-stimulated insulin release and apoptosis in human islets
    James D Johnson
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Cell Transplant 18:833-45. 2009
    ..Together, our results demonstrate that immunosuppressants impair human beta-cell function and survival, and that these defects can be circumvented to a certain extent with exenatide treatment...
  8. doi request reprint Mechanisms of pancreatic beta-cell apoptosis in diabetes and its therapies
    James D Johnson
    Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3
    Adv Exp Med Biol 654:447-62. 2010
    ..In this chapter, we review the factors that influence the propensity of beta-cells to undergo apoptosis and the mechanisms of this programmed cell death in the initiation and progression of diabetes...
  9. pmc Roles of IP3R and RyR Ca2+ channels in endoplasmic reticulum stress and beta-cell death
    Dan S Luciani
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Comlumbia, Canada
    Diabetes 58:422-32. 2009
    ..Here, we examined the effects of stimulating or inhibiting the ER-resident inositol trisphosphate receptors (IP(3)Rs) and the ryanodine receptors (RyRs) on the induction of beta-cell ER stress and apoptosis...
  10. ncbi request reprint RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets
    James D Johnson
    Department of Internal Medicine, Washington University, St Louis, Missouri 63110, USA
    J Biol Chem 279:24794-802. 2004
    ..Thus RyR2 activity seems to play an essential role in beta-cell survival in vitro by suppressing a death pathway mediated by calpain-10, a type 2 diabetes susceptibility gene with previously unknown function...
  11. doi request reprint Glucose and endoplasmic reticulum calcium channels regulate HIF-1beta via presenilin in pancreatic beta-cells
    Vardit Dror
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    J Biol Chem 283:9909-16. 2008
    ..These findings provide a mechanistic understanding of the signaling pathways activated when intracellular Ca(2+) homeostasis and metabolic activity are suppressed in diabetes and islet transplantation...
  12. doi request reprint Hyperinsulinemia drives diet-induced obesity independently of brain insulin production
    Arya E Mehran
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Cell Metab 16:723-37. 2012
    ..Thus, we provide genetic evidence that pathological circulating hyperinsulinemia drives diet-induced obesity and its complications...
  13. pmc Cardiomyocyte ATP production, metabolic flexibility, and survival require calcium flux through cardiac ryanodine receptors in vivo
    Michael J Bround
    Cardiovascular Research Group, Life Sciences Institute, University of British Columbia, Vancouver V6T 1Z3, Canada
    J Biol Chem 288:18975-86. 2013
    ..RYR2-mediated Ca(2+) fluxes are therefore proximal controllers of mitochondrial Ca(2+), ATP levels, and a cascade of transcription factors controlling metabolism and survival. ..
  14. pmc Carboxypeptidase E mediates palmitate-induced beta-cell ER stress and apoptosis
    Kristin D Jeffrey
    Diabetes Research Group, Laboratory of Molecular Signalling in Diabetes, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Proc Natl Acad Sci U S A 105:8452-7. 2008
    ..Thus, carboxypeptidase E degradation contributes to palmitate-induced beta-cell ER stress and apoptosis. CPE is a major link between hyperlipidemia and beta-cell death pathways in diabetes...
  15. ncbi request reprint Reduced expression of the insulin receptor in mouse insulinoma (MIN6) cells reveals multiple roles of insulin signaling in gene expression, proliferation, insulin content, and secretion
    Mitsuru Ohsugi
    Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:4992-5003. 2005
    ..These results demonstrate important roles of the insulin receptor in islet beta cell gene expression and function and may serve to elucidate molecular defects in animal models with diminished beta cell insulin signaling...
  16. ncbi request reprint Glucose-induced endothelial heparanase secretion requires cortical and stress actin reorganization
    Fang Wang
    Division of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146, East Mall, Vancouver, BC, Canada V6T 1Z3
    Cardiovasc Res 87:127-36. 2010
    ..Using high glucose (HG) to replicate hyperglycaemia observed following diabetes, the present study was designed to determine the mechanism by which HG initiates endothelial heparanase secretion...
  17. pmc Increased islet apoptosis in Pdx1+/- mice
    James D Johnson
    Renal Division, Department of Internal Medicine, Washington University School of Medicine Barnes Jewish Hospital, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Clin Invest 111:1147-60. 2003
    ....
  18. ncbi request reprint Ryanodine receptors in human pancreatic beta cells: localization and effects on insulin secretion
    James D Johnson
    Division of Metabolism, Department of Internal Medicine, Washington University School of Medicine, Box 8126, 8831 Wohl Clinic, 660 S Euclid, St Louis, MO 63110, USA
    FASEB J 18:878-80. 2004
    ....
  19. ncbi request reprint Kinetics and genomic profiling of adult human and mouse β-cell maturation
    Marta Szabat
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Islets 3:175-87. 2011
    ..The results have significant implications for islet regeneration and for in vitro generation of functional β-cells to treat diabetes...
  20. pmc Acute insulin signaling in pancreatic beta-cells is mediated by multiple Raf-1 dependent pathways
    Emilyn U Alejandro
    Department of Cellular and Physiological Sciences, University of British Columbia, 5358 Life Sciences Building, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada
    Endocrinology 151:502-12. 2010
    ..Together our findings identify a novel insulin signaling pathway in beta-cells and shed light on insulin's antiapoptotic and mitogenic mechanisms...
  21. pmc Defective insulin secretion and increased susceptibility to experimental diabetes are induced by reduced Akt activity in pancreatic islet beta cells
    Ernesto Bernal-Mizrachi
    Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 114:928-36. 2004
    ..These observations suggest that Akt plays a novel and important role in the regulation of distal components of the secretory pathway and that this enzyme represents a therapeutic target for improvement of beta cell function in diabetes...
  22. doi request reprint Maturation of adult beta-cells revealed using a Pdx1/insulin dual-reporter lentivirus
    Marta Szabat
    Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
    Endocrinology 150:1627-35. 2009
    ..These results have implications for harnessing the maturation process for therapeutic purposes...
  23. ncbi request reprint Improved human pancreatic islet isolation for a prospective cohort study of islet transplantation vs best medical therapy in type 1 diabetes mellitus
    Garth L Warnock
    Department of Surgery, British Columbia Islet Transplant Centre, University of British Columbia, 950 W 10th Avenue, Vancouver, BC, Canada V5Z 4E3
    Arch Surg 140:735-44. 2005
    ..A local multiorgan donor pancreas procurement program can provide a source for optimized isolation of purified viable islets for transplantation into patients with type 1 diabetes mellitus receiving best medical therapy...
  24. pmc Islet cholesterol accumulation due to loss of ABCA1 leads to impaired exocytosis of insulin granules
    Janine K Kruit
    Departments of Medical Genetics, Centre for Molecular Medicine, and Therapeutics, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada
    Diabetes 60:3186-96. 2011
    ..The ATP-binding cassette transporter A1 (ABCA1) is essential for normal insulin secretion from β-cells. The aim of this study was to elucidate the mechanisms underlying the impaired insulin secretion in islets lacking β-cell ABCA1...
  25. ncbi request reprint Fatty acid-induced nuclear translocation of heparanase uncouples glucose metabolism in endothelial cells
    Fang Wang
    Molecular and Cellular Pharmacology Group, Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146 East Mall, Vancouver, B C, Canada V6T 1Z3
    Arterioscler Thromb Vasc Biol 32:406-14. 2012
    ..We have recently reported that high fatty acid increased the nuclear content of endothelial heparanase. Here, we examined the mechanism and the consequences behind this nuclear translocation of heparanase...
  26. doi request reprint A multi-year analysis of islet transplantation compared with intensive medical therapy on progression of complications in type 1 diabetes
    Garth L Warnock
    Departments of Surgery, Ophthalmology and Visual Sciences, and Medicine, Ike Barber Human Islet Transplantation Laboratory, University of British Columbia, Vancouver, BC, Canada
    Transplantation 86:1762-6. 2008
    ..We hypothesized that transplantation of islets into type 1 diabetics could improve outcomes of glucose metabolism, renal function, retinopathy, and neuropathy compared with intensive medical therapy...
  27. pmc Intraislet SLIT-ROBO signaling is required for beta-cell survival and potentiates insulin secretion
    Yu Hsuan Carol Yang
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Proc Natl Acad Sci U S A 110:16480-5. 2013
    ..Because diabetes results from a deficiency in functional beta-cell mass, these studies may contribute to therapeutic approaches for improving beta-cell survival and function. ..
  28. pmc Loss of both ABCA1 and ABCG1 results in increased disturbances in islet sterol homeostasis, inflammation, and impaired β-cell function
    Janine K Kruit
    Department of Medical Genetics, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia, Canada
    Diabetes 61:659-64. 2012
    ..These data suggest that ABCA1 and ABCG1 each make complimentary and important contributions to β-cell function by maintaining islet cholesterol homeostasis in vivo...
  29. ncbi request reprint Differential regulation and localization of carboxypeptidase D and carboxypeptidase E in human and mouse β-cells
    Kwan Yi Chu
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Islets 3:155-65. 2011
    ..Taken together, our data demonstrate that CPE and CPD are differentially localized, differentially regulated and unlikely to have compensatory functions in pancreatic β-cells...
  30. pmc Autophagy regulates pancreatic beta cell death in response to Pdx1 deficiency and nutrient deprivation
    Kei Fujimoto
    Division of Endocrinology, Metabolism and Lipid Research, Department of Internal Medicine, Washington University School of Medicine Barnes Jewish Hospital, St Louis, Missouri 63110, USA
    J Biol Chem 284:27664-73. 2009
    ..The role of autophagy should be considered in studies of pancreatic beta cell death and diabetes and as a target for novel therapeutic intervention...
  31. ncbi request reprint Voltage-gated Ca(2+) influx and insulin secretion in human and mouse beta-cells are impaired by the mitochondrial Na(+)/Ca(2+) exchange inhibitor CGP-37157
    Dan S Luciani
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    Eur J Pharmacol 576:18-25. 2007
    ..Efforts to use CGP-37157 or design related compounds for therapeutic purposes should take these competing effects into account...
  32. pmc Bcl-2 and Bcl-xL suppress glucose signaling in pancreatic β-cells
    Dan S Luciani
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Diabetes 62:170-82. 2013
    ..Our work suggests that prosurvival Bcl proteins normally dampen the β-cell response to glucose and thus reveals these core apoptosis proteins as integrators of cell death and physiology in pancreatic β-cells...
  33. doi request reprint Nanospaces between endoplasmic reticulum and mitochondria as control centres of pancreatic β-cell metabolism and survival
    James D Johnson
    Department of Cellular and Physiological Sciences, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada
    Protoplasma 249:S49-58. 2012
    ..Many aspects of the mechanisms underlying this control system remain to be uncovered, and new nanotechnologies are required understand these domains at a molecular level...
  34. pmc Pancreatic β-cell Raf-1 is required for glucose tolerance, insulin secretion, and insulin 2 transcription
    Emilyn U Alejandro
    Laboratory of Molecular Signalling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
    FASEB J 25:3884-95. 2011
    ..This work provides the first direct evidence that Raf-1 signaling is essential for the regulation of basal insulin transcription and the supply of releasable insulin in vivo...
  35. doi request reprint Effects of palmitate on ER and cytosolic Ca2+ homeostasis in beta-cells
    Kamila S Gwiazda
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, 5358 Life Sciences Bldg, 2350 Health Sciences Mall, Vancouver, BC, Canada, V6T 1Z3
    Am J Physiol Endocrinol Metab 296:E690-701. 2009
    ..We present the first direct evidence that free fatty acids reduce ER Ca2+ and shed light on pathways involved in lipotoxicity and the pathogenesis of type 2 diabetes...
  36. pmc Insulin stimulates primary beta-cell proliferation via Raf-1 kinase
    Jennifer L Beith
    Laboratory of Molecular Signalling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    Endocrinology 149:2251-60. 2008
    ..These findings have significant implications for the understanding of the regulation of beta-cell mass in both the hyperinsulinemic and insulin-deficient states that occur in the various forms of diabetes...
  37. pmc Improving function and survival of pancreatic islets by endogenous production of glucagon-like peptide 1 (GLP-1)
    Rhonda D Wideman
    Laboratories of Molecular and Cellular Medicine, Departments of Cellular and Physiological Sciences and Surgery, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada
    Proc Natl Acad Sci U S A 103:13468-73. 2006
    ..These results demonstrate a unique strategy for liberating GLP-1 from directly within the target organ and highlight the potential for up-regulating islet GLP-1 production as a means of treating diabetes...
  38. doi request reprint Pancreatic cell immobilization in alginate beads produced by emulsion and internal gelation
    Corinne A Hoesli
    Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada
    Biotechnol Bioeng 108:424-34. 2011
    ....
  39. ncbi request reprint Acute effects of insulin on beta-cells from transplantable human islets
    Dan S Luciani
    Department of Cellular and Physiological Science, University of British Columbia, Vancouver, 2350 Health Sciences Mall, Vancouver, BC, Canada
    Mol Cell Endocrinol 241:88-98. 2005
    ..Our findings suggest autocrine insulin signaling directly controls insulin protein levels, but not exocytosis, in beta-cells and demonstrate the functional specificity of insulin signaling and glucose signaling in human islet cells...
  40. ncbi request reprint Beta-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment
    Liam R Brunham
    Department of Medical Genetics, Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia, 950 West 28th Avenue, Vancouver, British Columbia V5Z 4H4, Canada
    Nat Med 13:340-7. 2007
    ..These experiments establish a new role for Abca1 in beta-cell cholesterol homeostasis and insulin secretion, and suggest that cholesterol accumulation may contribute to beta-cell dysfunction in type 2 diabetes...
  41. pmc ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells
    Kwan Yi Chu
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 285:32606-15. 2010
    ..These findings add to the emerging body of evidence linking beta cell metabolism with programmed cell death...
  42. pmc MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion
    Marco Gallo
    Department of Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 6:e17827. 2011
    ..Our results suggest that controlling MISC-1/OGC function allows regulation of mitochondrial morphology and cell survival decisions by the metabolic needs of the cell...
  43. doi request reprint Rheb activates protein synthesis and growth in adult rat ventricular cardiomyocytes
    Yanni Wang
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
    J Mol Cell Cardiol 45:812-20. 2008
    ..Our findings reveal that Rheb itself can activate both protein synthesis and cell growth in ARVC and demonstrate the key role played by mTORC1 in the growth of cardiomyocytes...
  44. pmc Impaired Ca(2+) signaling in β-cells lacking leptin receptors by Cre-loxP recombination
    Eva Tudurí
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 8:e71075. 2013
    ..These findings highlight the need to generate improved Cre-driver mouse models or new tools to induce Cre recombination in β-cells. ..
  45. ncbi request reprint Role of the TLR signaling molecule TRIF in β-cell function and glucose homeostasis
    Meredith J H Hutton
    Department of Pathology and Laboratory Medicine, University of British Columbia, Child and Family Research Institute, Vancouver, Canada
    Islets 2:104-11. 2010
    ..These studies suggest a role for TLR signaling in glucose homeostasis, and raise the possibility that TRIF signaling is required for normal b-cell function...
  46. pmc Altered pancreatic growth and insulin secretion in WSB/EiJ mice
    Maggie M Ho
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 9:e88352. 2014
    ..These studies suggest that WSB mice may provide novel insight into mechanisms regulating insulin secretion and also highlight the role of post-natal growth in determining adult β-cell mass. ..
  47. doi request reprint Characterization of polyhormonal insulin-producing cells derived in vitro from human embryonic stem cells
    Jennifer E Bruin
    Laboratory of Molecular and Cellular Medicine, Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada
    Stem Cell Res 12:194-208. 2014
    ..2 may contribute to the observed KATP channel defects in hESC-derived islet endocrine cells, and along with lack of GLUT1, may explain the absence of glucose-stimulated insulin secretion. ..
  48. doi request reprint AMP-activated protein kinase confers protection against TNF-{alpha}-induced cardiac cell death
    Girish Kewalramani
    Division of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC, Canada
    Cardiovasc Res 84:42-53. 2009
    ....
  49. ncbi request reprint Ontogeny of ghrelin, obestatin, preproghrelin, and prohormone convertases in rat pancreas and stomach
    Pallavi Walia
    Endocrinology and Diabetes Unit, British Columbia Children s Hospital, Vancouver, British Columbia, V6H 3V4, Canada
    Pediatr Res 65:39-44. 2009
    ..In pancreas, the colocalization of PC1/3 or PC2 in ghrelin positive cells points to a role for both PCs in preproghrelin processing...
  50. ncbi request reprint An odyssey of islet transplantation for therapy of type 1 diabetes
    Garth L Warnock
    Department of Surgery, University of British Columbia Medical Center, 910 West 10th Avenue, Vancouver, British Columbia V5Z 4E3
    World J Surg 31:1569-76. 2007
    ..In this article, some seminal Canadian contributions to this field are reviewed, including contributions at the basic research laboratory and translational applications to bedside therapy of type 1 diabetes...
  51. pmc Diabetes genes identified by genome-wide association studies are regulated in mice by nutritional factors in metabolically relevant tissues and by glucose concentrations in islets
    Maggie M Ho
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    BMC Genet 14:10. 2013
    ..Thus, to understand how these new genetic variants associated with diabetes risk may act, it is necessary to understand the regulation of their cognate genes...
  52. pmc Cardiac ryanodine receptors control heart rate and rhythmicity in adult mice
    Michael J Bround
    Cardiovascular Research Group, Life Sciences Institute, University of British Columbia, 5358 Life Sciences Building, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
    Cardiovasc Res 96:372-80. 2012
    ..The aim of the present study was to test the hypothesis that RYR2 loss-of-function would reduce heart rate and rhythmicity in vivo...
  53. pmc Purified human pancreatic duct cell culture conditions defined by serum-free high-content growth factor screening
    Corinne A Hoesli
    Michael Smith Laboratories and Department of Biological and Chemical Engineering, University of British Columbia, Vancouver, Canada
    PLoS ONE 7:e33999. 2012
    ..The methods developed in this work should advance pancreatic cancer and diabetes research by providing effective cell culture and high-throughput screening platforms to study purified primary pancreatic CK19+ cells...
  54. pmc Inhibition of Raf-1 alters multiple downstream pathways to induce pancreatic beta-cell apoptosis
    Emilyn U Alejandro
    Laboratory of Molecular Signaling in Diabetes, Diabetes Research Group, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 283:2407-17. 2008
    ..These studies identify Raf-1 as a critical anti-apoptotic kinase in pancreatic beta-cells and contribute to our understanding of survival signaling in this cell type...
  55. ncbi request reprint Multi-parameter single-cell kinetic analysis reveals multiple modes of cell death in primary pancreatic β-cells
    Yu Hsuan Carol Yang
    Department of Cellular and Physiological Sciences, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver BC V6T 1Z3, Canada
    J Cell Sci 126:4286-95. 2013
    ..We conclude that apoptosis is not the primary mode of adult primary β-cell death...
  56. doi request reprint Multiparameter screening reveals a role for Na+ channels in cytokine-induced β-cell death
    Yu Hsuan Carol Yang
    Department of Cellular and Physiological Sciences Y H C Y, J D J, Department of Anesthesiology, Pharmacology, and Therapeutics Y Y V, H T K, and Department of Biochemistry and Molecular Biology M R, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Endocrinol 28:406-17. 2014
    ..Carbamazepine down-regulated the proapoptotic and endoplasmic reticulum stress signaling induced by cytokines. Together, these studies point to Na(+) channels as a novel therapeutic target in diabetes...
  57. pmc Nicotinic acid-adenine dinucleotide phosphate-sensitive calcium stores initiate insulin signaling in human beta cells
    James D Johnson
    Department of Internal Medicine, Renal Division, Barnes Jewish Hospital, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 99:14566-71. 2002
    ..These data reveal the complexity of insulin signal transduction and function in human beta cells and demonstrate functional NAADP-sensitive Ca(2+) stores in a human primary cultured cell type...