Research Topics
Genomes and Genes | Martin HolcikSummaryAffiliation: University of Ottawa Country: Canada Publications
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Publications
Conditionally lethal genes in the N pilus region of plasmid pCU1M Holcik
Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Ontario, K1S 5B6, Canada
Plasmid 42:53-9. 1999..kilC expression in E. coli leads to cell death accompanied by an increase in average cell length without affecting septum formation...
Translational control in stress and apoptosisMartin Holcik
Apoptosis Research Center, Room R3116, Children s Hospital of Eastern Ontario, 401 Smyth Road, Ottawa, ON, K1H 8L1, Canada
Nat Rev Mol Cell Biol 6:318-27. 2005....
Targeting endogenous inhibitors of apoptosis for treatment of cancer, stroke and multiple sclerosisMartin Holcik
Apoptosis Research Center, Children s Hospital of Eastern Ontario Research Institute and Department of Pediatrics, University of Ottawa, 401 Smyth Road, Ottawa, Ontario, K1H 8L1, USA
Expert Opin Ther Targets 8:241-53. 2004..Indeed, available data suggest that the therapeutic downregulation of IAPs by antisense targeting or their adenovirally-mediated overexpression, can in fact be used to successfully modulate cell death...
Targeting translation for treatment of cancer--a novel role for IRES?Martin Holcik
Apoptosis Research Center, Children s Hospital of Eastern Ontario Research Institute, 401 Smyth Road, Ottawa, ON K1H 8L1 Canada
Curr Cancer Drug Targets 4:299-311. 2004....
Translational upregulation of the X-linked inhibitor of apoptosisMartin Holcik
Solange Gauthier Karsh Molecular Genetics Laboratory, Department of Pediatrics, Children s Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, K1H 8L1 Canada
Ann N Y Acad Sci 1010:249-58. 2003..The IRES regulation of XIAP translation points to an important mechanism in the control and regulation of apoptosis...
The internal ribosome entry site-mediated translation of antiapoptotic protein XIAP is modulated by the heterogeneous nuclear ribonucleoproteins C1 and C2Martin Holcik
Solange Gauthier Karsh Molecular Genetics Laboratory, Children s Hospital of Eastern Ontario Research Institute, Ottawa, Canada
Mol Cell Biol 23:280-8. 2003..Given the central role of XIAP in the regulation of apoptosis these results are important for our understanding of the control of apoptosis...
The IAP proteinsMartin Holcik
Department of Pediatrics, University of Ottawa, Children's Hospital of Eastern Ontario Research Institute, Room R310, 401 Smyth Rd, Ottawa, Ontario, Canada K1H 8L1
Trends Genet 18:537. 2002
XIAP, the guardian angelM Holcik
Children s Hospital of Eastern Ontario Research Institute, and AEgera Oncology Inc, 401 Smyth Road, Ottawa, Ontario K1H 8L1, Canada
Nat Rev Mol Cell Biol 2:550-6. 2001..One cellular inhibitor of apoptosis, XIAP, has emerged as a crucial regulator of caspases, and is itself subject to complex negative regulation...
Internal ribosome initiation of translation and the control of cell deathM Holcik
Solange Gauthier Karsh Molecular Genetics Laboratory, Children s Hospital of Eastern Ontario Research Institute, 401 Smyth Road, ON, K1H 8L1, Ottawa, Canada
Trends Genet 16:469-73. 2000..Thus, IRES-mediated translational control might have evolved to regulate cellular responses in acute but transient stress conditions that would otherwise lead to cell death...
The eIF4G homolog DAP5/p97 supports the translation of select mRNAs during endoplasmic reticulum stressStephen M Lewis
Apoptosis Research Centre, Children s Hospital of Eastern Ontario, 401 Smyth Road, Ottawa, Ontario, K1H 8L1 Canada
Nucleic Acids Res 36:168-78. 2008..Thus, DAP5/p97 is a translational activator that selectively modulates translation of specific mRNAs during conditions of cellular stress in both a caspase-dependent and caspase-independent manner...
Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translationStephen M Lewis
Apoptosis Research Centre, Children s Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa, Ontario, K1H 8L1, Canada
Mol Biol Cell 18:1302-11. 2007..Our findings demonstrate that cytoplasmic hnRNP A1 is a negative regulator of XIAP IRES-dependent translation, indicating a novel function for the cytoplasmic form of this protein...
A search for structurally similar cellular internal ribosome entry sitesStephen D Baird
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
Nucleic Acids Res 35:4664-77. 2007..We therefore propose that cellular IRES are not defined by overall structure, as viral IRES, but are instead dependent upon short motifs and trans-acting factors for their function...
The utrophin A 5'-untranslated region confers internal ribosome entry site-mediated translational control during regeneration of skeletal muscle fibersPedro Miura
Department of Cellular and Molecular Medicine and Centre for Neuromuscular Disease, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
J Biol Chem 280:32997-3005. 2005..These findings provide a novel target for up-regulating levels of utrophin A in Duchenne muscular dystrophy muscle fibers via pharmacological interventions...
IRES-mediated translation of utrophin A is enhanced by glucocorticoid treatment in skeletal muscle cellsPedro Miura
Department of Cellular and Molecular Medicine, Centre for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada
PLoS ONE 3:e2309. 2008..Targeting the utrophin A IRES may thus offer an important and novel therapeutic avenue for developing drugs appropriate for DMD patients...
Internal ribosome entry site-mediated translation of Apaf-1, but not XIAP, is regulated during UV-induced cell deathNicoleta Hosszu Ungureanu
Apoptosis Research Center, Children s Hospital of Eastern Ontario, Ottawa, Ontario K1H 8L1
J Biol Chem 281:15155-63. 2006..These data suggest that progression of UV-induced apoptosis requires IRES-mediated translation of Apaf-1 to ensure continuous levels of Apaf-1 despite an overall suppression of protein synthesis...
p38 Mitogen-activated protein kinase stabilizes SMN mRNA through RNA binding protein HuRFaraz Farooq
University of Ottawa, Ottawa K1H 8M5, Canada
Hum Mol Genet 18:4035-45. 2009....
Spurious splicing within the XIAP 5' UTR occurs in the Rluc/Fluc but not the betagal/CAT bicistronic reporter systemMartin Holcik
Apoptosis Research Center, Children s Hospital of Eastern Ontario, Ottawa, Canada
RNA 11:1605-9. 2005..In this paper, we demonstrate that the splicing within the XIAP 5' UTR specifically occurs only in the context of mRNA produced from the Rluc/Fluc but not the pbetagal/CAT bicistronic reporter plasmid...
Translational induction of the inhibitor of apoptosis protein HIAP2 during endoplasmic reticulum stress attenuates cell death and is mediated via an inducible internal ribosome entry site elementDinesh Warnakulasuriyarachchi
Apoptosis Research Center, Children s Hospital of Eastern Ontario, Department of Pediatrics, University of Ottawa, Ottawa, Ontario K1H 8L1, Canada
J Biol Chem 279:17148-57. 2004..These data suggest an existence of a novel mechanism that regulates apoptotic response in ER stress...
Prolactin increases SMN expression and survival in a mouse model of severe spinal muscular atrophy via the STAT5 pathwayFaraz Farooq
University of Ottawa, Ottawa, Canada
J Clin Invest 121:3042-50. 2011..Our results confirm earlier work suggesting STAT5 pathway activators as potential therapeutic compounds for the treatment of SMA and identify PRL as one such promising agent...
Inhibitor of apoptosis protein cIAP2 is essential for lipopolysaccharide-induced macrophage survivalDamiano Conte
Apoptosis Research Center, Children s Hospital of Eastern Ontario, 401 Smyth Rd, Rm R3114, Ottawa, Ontario K1H 8L1, Canada
Mol Cell Biol 26:699-708. 2006..Hence, cIAP2 is critical in the maintenance of a normal innate immune inflammatory response...
Perk-dependent translational regulation promotes tumor cell adaptation and angiogenesis in response to hypoxic stressJaime D Blais
Ottawa Health Research Institute, Ottawa, ON K1H 1C4, Canada
Mol Cell Biol 26:9517-32. 2006....
Cap-independent regulation of gene expression in apoptosisTyson E Graber
Apoptosis Research Centre, Children s Hospital of Eastern Ontario, Room 3116, 401 Smyth Road, Ottawa, Ontario, Canada K1H 8L1
Mol Biosyst 3:825-34. 2007..In addition, recent advances towards our understanding of the physiological role and mechanism of IRES-mediated translation in the context of cell stress-induced apoptosis and human disease will be examined...
Searching for IRESStephen D Baird
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ontario K1H 8M5, Canada
RNA 12:1755-85. 2006....
Nucleotide Composition of Cellular Internal Ribosome Entry Sites Defines Dependence on NF45 and Predicts a Posttranscriptional Mitotic RegulonMame Daro Faye
Apoptosis Research Centre, Children s Hospital of Eastern Ontario, and University of Ottawa, Ottawa, Ontario, Canada
Mol Cell Biol 33:307-18. 2013..This study uncovers a novel role for NF45 in regulating ploidy and highlights the importance of IRES-mediated translation in cellular homeostasis...
Distinct 5' UTRs regulate XIAP expression under normal growth conditions and during cellular stressAlura Riley
Apoptosis Research Centre, Children s Hospital of Eastern Ontario, Ottawa, Canada
Nucleic Acids Res 38:4665-74. 2010....
Translation mechanism and regulation: old players, new concepts. Meeting on translational control and non-coding RNAMartin Holcik
Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth Road, Ottawa, Ontario K1H 8L1, Canada
EMBO Rep 8:639-43. 2007
An oxygen-regulated switch in the protein synthesis machineryJames Uniacke
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
Nature 486:126-9. 2012..These findings demonstrate that cells have evolved a program by which oxygen tension switches the basic translation initiation machinery...
Tumor suppressor PDCD4 represses internal ribosome entry site-mediated translation of antiapoptotic proteins and is regulated by S6 kinase 2Urszula Liwak
Apoptosis Research Centre, Children s Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada
Mol Cell Biol 32:1818-29. 2012....
Strong eukaryotic IRESs have weak secondary structureXuhua Xia
Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Canada
PLoS ONE 4:e4136. 2009..The objective of this work was to investigate the hypothesis that eukaryotic Internal Ribosome Entry Sites (IRES) lack secondary structure and to examine the generality of the hypothesis...
RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alphaStefanie Galban
LCMB, NIA, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
Mol Cell Biol 28:93-107. 2008..Conversely, HIF-1alpha expression and translation in response to CoCl(2) were markedly elevated after HuR overexpression. We propose that HuR and PTB jointly upregulate HIF-1alpha translation in response to CoCl(2)...
Cytoplasmic relocalization of heterogeneous nuclear ribonucleoprotein A1 controls translation initiation of specific mRNAsAnne Cammas
Institut National de la Sante et de la Recherche Medicale U563, Toulouse, F 31000, France
Mol Biol Cell 18:5048-59. 2007..Our data highlight a new way to regulate protein synthesis in eukaryotes through the subcellular relocalization of a nuclear mRNA-binding protein...
Translational regulation of x-linked inhibitor of apoptosis protein by interleukin-6: a novel mechanism of tumor cell survivalYoko Yamagiwa
Scott and White Clinic, College of Medicine, Texas A and M University System Health Science Center, 2401 South 31st Street, Temple, TX 76502, USA
Cancer Res 64:1293-8. 2004....
