John J Heikkila
Affiliation: University of Waterloo
Campbell J, Heikkila J. Effect of hemin, baicalein and heme oxygenase-1 (HO-1) enzyme activity inhibitors on Cd-induced accumulation of HO-1, HSPs and aggresome-like structures in Xenopus kidney epithelial cells. Comp Biochem Physiol C Toxicol Pharmacol. 2018;210:1-17 pubmed publisher
..These results suggest that HO-1-mediated protection against CdCl2 toxicity includes the maintenance of actin cytoskeletal and microtubular structure and the suppression of aggregated protein and aggresome-like structures. ..
Heikkila J. The expression and function of hsp30-like small heat shock protein genes in amphibians, birds, fish, and reptiles. Comp Biochem Physiol A Mol Integr Physiol. 2017;203:179-192 pubmed publisher
..The current availability of probes to detect the presence of hsp30 mRNA or encoded protein has resulted in the increased use of hsp30 gene expression as a marker of proteotoxic stress in non-mammalian vertebrates. ..
Music E, Khan S, Khamis I, Heikkila J. Accumulation of heme oxygenase-1 (HSP32) in Xenopus laevis A6 kidney epithelial cells treated with sodium arsenite, cadmium chloride or proteasomal inhibitors. Comp Biochem Physiol C Toxicol Pharmacol. 2014;166:75-87 pubmed publisher
..An examination of heavy metal and proteasomal inhibitor-induced HO-1 accumulation in amphibians is of importance given the presence of toxic heavy metals in aquatic habitats. ..
Khamis I, Chan D, Shirriff C, Campbell J, Heikkila J. Expression and localization of the Xenopus laevis small heat shock protein, HSPB6 (HSP20), in A6 kidney epithelial cells. Comp Biochem Physiol A Mol Integr Physiol. 2016;201:12-21 pubmed publisher
..These findings were in contrast to HSP70, which was enhanced in response to all three stressors. Finally, immunocytochemical analysis revealed that HSPB6 was present in the cytoplasm in the perinuclear region with some in the nucleus. ..
Khan S, Heikkila J. Distinct patterns of HSP30 and HSP70 degradation in Xenopus laevis A6 cells recovering from thermal stress. Comp Biochem Physiol A Mol Integr Physiol. 2014;168:1-10 pubmed publisher
Shirriff C, Heikkila J. Characterization of cadmium chloride-induced BiP accumulation in Xenopus laevis A6 kidney epithelial cells. Comp Biochem Physiol C Toxicol Pharmacol. 2017;191:117-128 pubmed publisher
..In amphibians, the association of BiP with unfolded protein and its possible role in aggresome function may be vital in the maintenance of cellular proteostasis. ..
Khamis I, Heikkila J. Effect of isothiocyanates, BITC and PEITC, on stress protein accumulation, protein aggregation and aggresome-like structure formation in Xenopus A6 kidney epithelial cells. Comp Biochem Physiol C Toxicol Pharmacol. 2018;204:1-13 pubmed publisher
..This latter finding suggests that BITC-induced microtubule fragmentation may impede the movement of aggregated protein via microtubules and their subsequent coalescence into aggresome-like structures in the perinuclear region. ..
Khan S, Khamis I, Heikkila J. The small heat shock protein, HSP30, is associated with aggresome-like inclusion bodies in proteasomal inhibitor-, arsenite-, and cadmium-treated Xenopus kidney cells. Comp Biochem Physiol A Mol Integr Physiol. 2015;189:130-40 pubmed publisher
..While the role of HSP30 in these aggresome-like structures is not known, it is possible that they may be involved in various aspects of aggresome-like inclusion body formation or transport. ..