George Harauz

Summary

Affiliation: University of Guelph
Country: Canada

Publications

  1. pmc The effects of threonine phosphorylation on the stability and dynamics of the central molecular switch region of 18.5-kDa myelin basic protein
    Kenrick A Vassall
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    PLoS ONE 8:e68175. 2013
  2. pmc Myelin management by the 18.5-kDa and 21.5-kDa classic myelin basic protein isoforms
    George Harauz
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group and Collaborative Program in Neuroscience, University of Guelph, Guelph, Ontario, Canada
    J Neurochem 125:334-61. 2013
  3. ncbi request reprint Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis
    George Harauz
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, Room 230, Axelrod Building, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Micron 35:503-42. 2004
  4. ncbi request reprint Three-dimensional cryoelectron microscopic reconstruction of the 2.25-MDa homomultimeric phosphoenolpyruvate synthase from Staphylothermus marinus
    G Harauz
    Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada
    Biochem Biophys Res Commun 241:599-605. 1997
  5. ncbi request reprint A tale of two citrullines--structural and functional aspects of myelin basic protein deimination in health and disease
    George Harauz
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1
    Neurochem Res 32:137-58. 2007
  6. ncbi request reprint The classic basic protein of myelin--conserved structural motifs and the dynamic molecular barcode involved in membrane adhesion and protein-protein interactions
    George Harauz
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Curr Protein Pept Sci 10:196-215. 2009
  7. pmc Proline substitutions and threonine pseudophosphorylation of the SH3 ligand of 18.5-kDa myelin basic protein decrease its affinity for the Fyn-SH3 domain and alter process development and protein localization in oligodendrocytes
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 90:28-47. 2012
  8. doi request reprint Secondary structure and solvent accessibility of a calmodulin-binding C-terminal segment of membrane-associated myelin basic protein
    Lopamudra Homchaudhuri
    Department of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Biochemistry 49:8955-66. 2010
  9. doi request reprint Zinc induces disorder-to-order transitions in free and membrane-associated Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2: a solution CD and solid-state ATR-FTIR study
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
    Amino Acids 40:1485-502. 2011
  10. doi request reprint Phosphorylation of Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 facilitates cation-induced conformational changes and actin assembly
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochemistry 50:9587-604. 2011

Collaborators

Detail Information

Publications71

  1. pmc The effects of threonine phosphorylation on the stability and dynamics of the central molecular switch region of 18.5-kDa myelin basic protein
    Kenrick A Vassall
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    PLoS ONE 8:e68175. 2013
    ..The results support the hypothesis that the central conserved segment of MBP constitutes a molecular switch in which the conformation and/or intermolecular interactions are mediated by phosphorylation/dephosphorylation at T92 and T95. ..
  2. pmc Myelin management by the 18.5-kDa and 21.5-kDa classic myelin basic protein isoforms
    George Harauz
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group and Collaborative Program in Neuroscience, University of Guelph, Guelph, Ontario, Canada
    J Neurochem 125:334-61. 2013
    ....
  3. ncbi request reprint Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis
    George Harauz
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, Room 230, Axelrod Building, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Micron 35:503-42. 2004
    ..By solving the 3D structure of 18.5 kDa MBP and the effects of PTMs, we will attain a better understanding of myelin architecture, and of the molecular mechanisms that transpire in demyelinating diseases such as multiple sclerosis...
  4. ncbi request reprint Three-dimensional cryoelectron microscopic reconstruction of the 2.25-MDa homomultimeric phosphoenolpyruvate synthase from Staphylothermus marinus
    G Harauz
    Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada
    Biochem Biophys Res Commun 241:599-605. 1997
    ....
  5. ncbi request reprint A tale of two citrullines--structural and functional aspects of myelin basic protein deimination in health and disease
    George Harauz
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1
    Neurochem Res 32:137-58. 2007
    ..In this review, we describe the structural and functional consequences of MBP deimination in healthy and diseased myelin...
  6. ncbi request reprint The classic basic protein of myelin--conserved structural motifs and the dynamic molecular barcode involved in membrane adhesion and protein-protein interactions
    George Harauz
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Curr Protein Pept Sci 10:196-215. 2009
    ..For the most part, any classic protein isoform functions as an entropic spring that interacts in its entirety with membranes and cytoskeletal proteins, but the central and C-terminal motifs may represent molecular switches...
  7. pmc Proline substitutions and threonine pseudophosphorylation of the SH3 ligand of 18.5-kDa myelin basic protein decrease its affinity for the Fyn-SH3 domain and alter process development and protein localization in oligodendrocytes
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 90:28-47. 2012
    ....
  8. doi request reprint Secondary structure and solvent accessibility of a calmodulin-binding C-terminal segment of membrane-associated myelin basic protein
    Lopamudra Homchaudhuri
    Department of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada
    Biochemistry 49:8955-66. 2010
    ..The deiminated form would be more susceptible to regulation of its membrane binding functions by Ca(2+)-CaM than the unmodified form...
  9. doi request reprint Zinc induces disorder-to-order transitions in free and membrane-associated Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2: a solution CD and solid-state ATR-FTIR study
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
    Amino Acids 40:1485-502. 2011
    ....
  10. doi request reprint Phosphorylation of Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 facilitates cation-induced conformational changes and actin assembly
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochemistry 50:9587-604. 2011
    ..These results support the hypothesis that dehydrins stabilize the cytoskeleton under stress conditions and further that phosphorylation may be an important feature of this stabilization...
  11. ncbi request reprint An immunodominant epitope of myelin basic protein is an amphipathic alpha-helix
    Ian R Bates
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 279:5757-64. 2004
    ..The alpha-helix is tilted approximately 9 degrees, and the central lysine is in an ideal snorkeling position for side-chain interaction with the negatively charged phospholipid head groups...
  12. ncbi request reprint Terminal deletion mutants of myelin basic protein: new insights into self-association and phospholipid interactions
    Christopher M D Hill
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Ont, Canada
    Micron 34:25-37. 2003
    ....
  13. doi request reprint Myelin basic protein as a "PI(4,5)P2-modulin": a new biological function for a major central nervous system protein
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada, N1G 2W1
    Biochemistry 47:10372-82. 2008
    ..This regulation of PI(4,5)P 2 availability at the cell cortex may be coupled to the elaboration and outgrowth of the membranous cellular processes by oligodendrocytes...
  14. doi request reprint NMR assignment of an intrinsically disordered protein under physiological conditions: the 18.5 kDa isoform of murine myelin basic protein
    David S Libich
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group, University of Guelph, Guelph, ON, Canada
    Biomol NMR Assign 1:61-3. 2007
    ..We report the NMR assignment of 18.5 kDa recombinant murine myelin basic protein (MBP) in 100 mM KCl as a prerequisite to structural analyses of its Ca2+-dependent interaction with calmodulin...
  15. ncbi request reprint The interaction of zinc with membrane-associated 18.5 kDa myelin basic protein: an attenuated total reflectance-Fourier transform infrared spectroscopic study
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
    Amino Acids 39:739-50. 2010
    ....
  16. ncbi request reprint Charge effects modulate actin assembly by classic myelin basic protein isoforms
    Christopher M D Hill
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group, University of Guelph, Guelph, Ont, Canada N1G 2W1
    Biochem Biophys Res Commun 329:362-9. 2005
    ..The presence of at least one region derived from exon II or VI of the "classic" MBP gene was required for effective bundling as assessed by light scattering and transmission electron microscopy...
  17. ncbi request reprint Myelin basic protein has multiple calmodulin-binding sites
    David S Libich
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Biochem Biophys Res Commun 308:313-9. 2003
    ..We conclude that there is an additional CaM-binding site on MBP in a central segment (we posit murine residues 82-93) that forms an amphipathic alpha-helix...
  18. pmc Structured functional domains of myelin basic protein: cross talk between actin polymerization and Ca(2+)-dependent calmodulin interaction
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    Biophys J 101:1248-56. 2011
    ..These three domains of MBP represent molecular recognition fragments with multiple roles in both membrane- and protein-association...
  19. pmc Interaction of the 18.5-kD isoform of myelin basic protein with Ca2+ -calmodulin: effects of deimination assessed by intrinsic Trp fluorescence spectroscopy, dynamic light scattering, and circular dichroism
    David S Libich
    Department of Molecular Biology and Genetics, Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Protein Sci 12:1507-21. 2003
    ....
  20. ncbi request reprint Expression and properties of the recombinant murine Golli-myelin basic protein isoform J37
    Jaspreet Kaur
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 71:777-84. 2003
    ..It was determined via a gel-shift assay and fluorescence spectroscopy that rmJ37 and CaM interacted in a 1:1 ratio in a Ca(2+)-dependent manner. However, the interaction was weak compared with 18.5 kDa MBP...
  21. pmc Solid-state NMR spectroscopy of membrane-associated myelin basic protein--conformation and dynamics of an immunodominant epitope
    Mumdooh A M Ahmed
    Department of Physics, University of Guelph, Guelph, Ontario, Canada
    Biophys J 99:1247-55. 2010
    ..These results are the first, to our knowledge, to provide atomic-level detail of a membrane-anchoring segment of MBP, and direct evidence of decreased MBP-membrane interaction after posttranslational modification...
  22. ncbi request reprint Partitioning of myelin basic protein into membrane microdomains in a spontaneously demyelinating mouse model for multiple sclerosis
    Lillian S DeBruin
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol 84:993-1005. 2006
    ....
  23. ncbi request reprint Solution NMR structure of an immunodominant epitope of myelin basic protein. Conformational dependence on environment of an intrinsically unstructured protein
    Christophe Fares
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, Canada
    FEBS J 273:601-14. 2006
    ..The high sensitivity of the epitope structure to its environment is characteristic of intrinsically unstructured proteins, like MBP, and reflects its association with diverse ligands such as lipids and other proteins...
  24. ncbi request reprint Solution NMR and CD spectroscopy of an intrinsically disordered, peripheral membrane protein: evaluation of aqueous and membrane-mimetic solvent conditions for studying the conformational adaptability of the 18.5 kDa isoform of myelin basic protein (MBP)
    David S Libich
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, ON, Canada
    Eur Biophys J 37:1015-29. 2008
    ..5 at 300 K, and 100 mM KCl, pH 6.5 at 277 K were the best conditions to use for future solution NMR studies of MBP. Micelles of DPC were found to be inappropriate for backbone resonance assignments of rmMBP in this instance...
  25. pmc Deimination of membrane-bound myelin basic protein in multiple sclerosis exposes an immunodominant epitope
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Proc Natl Acad Sci U S A 103:4422-7. 2006
    ..These findings suggest a mechanism for initial loss of myelin stability and the autoimmune pathogenesis of multiple sclerosis...
  26. doi request reprint Interaction of myelin basic protein with actin in the presence of dodecylphosphocholine micelles
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Biochemistry 49:6903-15. 2010
    ..Thus, whereas the N-terminal domain of MBP can be more important for the MBP's actin polymerization activity and membrane-association, the C-terminal domain can regulate its interaction with actin...
  27. doi request reprint Myelin basic protein binds microtubules to a membrane surface and to actin filaments in vitro: effect of phosphorylation and deimination
    Joan M Boggs
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, Toronto, ON, Canada
    Biochim Biophys Acta 1808:761-73. 2011
    ..Thus, MBP may also cross-link microtubules to actin filaments in vivo...
  28. pmc Classical 18.5-and 21.5-kDa isoforms of myelin basic protein inhibit calcium influx into oligodendroglial cells, in contrast to golli isoforms
    Graham S T Smith
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 89:467-80. 2011
    ....
  29. doi request reprint Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
    Neurosci Lett 450:32-6. 2009
    ..The results lend further support to MBP's multifunctionality, particularly as an additional modulator of PI(4,5)P(2) availability in myelin...
  30. doi request reprint Misincorporation of the proline homologue Aze (azetidine-2-carboxylic acid) into recombinant myelin basic protein
    Kyrylo Bessonov
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    Phytochemistry 71:502-7. 2010
    ....
  31. doi request reprint Kinetics of human peptidylarginine deiminase 2 (hPAD2)--reduction of Ca2+ dependence by phospholipids and assessment of proposed inhibition by paclitaxel side chains
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol 86:437-47. 2008
    ..Despite paclitaxel having previously been shown to inhibit hPAD2 in vitro, the side chain of paclitaxel alone did not inhibit this enzyme's activity...
  32. pmc Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 91:349-62. 2013
    ....
  33. doi request reprint Interactions of Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes at cold and ambient temperatures-surface morphology and single-molecule force measurements show phase separation, and reveal tertiary and quaternary associations
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada
    Biochim Biophys Acta 1828:967-80. 2013
    ..Single-molecule force spectroscopy of both proteins pulled from supported lipid bilayers indicated the induced formation of tertiary conformations in both proteins, and potentially a dimeric association for TsDHN-2...
  34. ncbi request reprint Membrane-anchoring and charge effects in the interaction of myelin basic protein with lipid bilayers studied by site-directed spin labeling
    Ian R Bates
    Department of Molecular Biology and Genetics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 278:29041-7. 2003
    ..The mobility at F42A/F43A and especially F86A/F87A increased significantly. Depth measurements and helical wheel analysis indicated that the Phe-86/Phe-87 region could form a surface-seeking amphipathic alpha-helix...
  35. pmc Classic 18.5- and 21.5-kDa myelin basic protein isoforms associate with cytoskeletal and SH3-domain proteins in the immortalized N19-oligodendroglial cell line stimulated by phorbol ester and IGF-1
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada
    Neurochem Res 37:1277-95. 2012
    ..This study supports a role for classic MBP isoforms in cytoskeletal and other protein-protein interactions during membrane and cytoskeletal remodeling in OLGs...
  36. pmc Induced secondary structure and polymorphism in an intrinsically disordered structural linker of the CNS: solid-state NMR and FTIR spectroscopy of myelin basic protein bound to actin
    Mumdooh A M Ahmed
    Department of Physics, University of Guelph, Guelph, Canada
    Biophys J 96:180-91. 2009
    ..The overall conformational polymorphism of MBP is consistent with its in vivo roles as both a linker (membranes and cytoskeleton) and a putative signaling hub...
  37. ncbi request reprint The BG21 isoform of Golli myelin basic protein is intrinsically disordered with a highly flexible amino-terminal domain
    Mumdooh A M Ahmed
    Department of Physics and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Biochemistry 46:9700-12. 2007
    ..Another highly mobile segment, A126-S127-G128-G129, may function as a hinge...
  38. doi request reprint Structural polymorphism and multifunctionality of myelin basic protein
    George Harauz
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Biochemistry 48:8094-104. 2009
    ....
  39. doi request reprint Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)--characterization of its phosphatase activity and interaction with Golli-BG21
    Vladimir V Bamm
    Departments of Molecular and Cellular Biology, Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    Protein Expr Purif 62:36-43. 2008
    ..Using glutaraldehyde cross-linking and gel shift assays, the rmBG21-DeltaN-rmGIP interaction was shown to be equimolar and specific, but seemingly relatively weak, suggesting that a third interaction partner is required in vivo...
  40. ncbi request reprint Molecular "negativity" may underlie multiple sclerosis: role of the myelin basic protein family in the pathogenesis of MS
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology, Biophysics Interdepartmental Group, University of Guelph, Ontario N1G 2W1, Canada
    Int Rev Neurobiol 79:149-72. 2007
    ..In this chapter, we describe the structural and functional consequences of MBP deimination in healthy and diseased myelin...
  41. ncbi request reprint An Arg/Lys-->Gln mutant of recombinant murine myelin basic protein as a mimic of the deiminated form implicated in multiple sclerosis
    Ian R Bates
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    Protein Expr Purif 25:330-41. 2002
    ..Overall, the mutant protein's properties were consistent with the effects of deimination and support its use as a model for evaluating the effects of this modification...
  42. ncbi request reprint Assembly of tubulin by classic myelin basic protein isoforms and regulation by post-translational modification
    Christopher M D Hill
    Department of Molecular and Cellular Biology and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
    Biochemistry 44:16672-83. 2005
    ..Finally, bC1 was shown to disrupt and aggregate planar sheets of crystalline tubulin stabilized by paclitaxel, establishing that these structures are not suitable substrates for the formation of MBP cocrystals...
  43. doi request reprint Solution nuclear magnetic resonance structure and molecular dynamics simulations of a murine 18.5 kDa myelin basic protein segment (S72-S107) in association with dodecylphosphocholine micelles
    Mumdooh A M Ahmed
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Biochemistry 51:7475-87. 2012
    ....
  44. pmc The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal
    Graham S T Smith
    Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    Biochem Biophys Res Commun 422:670-5. 2012
    ....
  45. ncbi request reprint Purification and spectroscopic characterization of the recombinant BG21 isoform of murine golli myelin basic protein
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada
    J Neurosci Res 85:272-84. 2007
    ..Thus, like "classic" MBP and the Golli-MBP isoform J37, rmBG21 is intrinsically disordered, implying multi functionality, and that its conformation depends on its environment and bound ligands...
  46. pmc Peptidylarginine deiminase 2 (PAD2) overexpression in transgenic mice leads to myelin loss in the central nervous system
    Abdiwahab A Musse
    Department of Molecular and Cellular Biology and Biophysics Interdepartmental Group, University of Guelph, Ontario, Canada N1G 2W1
    Dis Model Mech 1:229-40. 2008
    ..These data support the hypothesis that elevated PAD levels in white matter represents an early change that precedes demyelination...
  47. pmc Solid-state NMR spectroscopy of 18.5 kDa myelin basic protein reconstituted with lipid vesicles: spectroscopic characterisation and spectral assignments of solvent-exposed protein fragments
    Ligang Zhong
    Department of Physics, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1
    Biochim Biophys Acta 1768:3193-205. 2007
    ..Chemical shift index analysis showed that the fragments were largely disordered under these conditions. These combined approaches are applicable to ssNMR investigations of other peripheral membrane proteins reconstituted with lipids...
  48. pmc Backbone dynamics of the 18.5 kDa isoform of myelin basic protein reveals transient alpha-helices and a calmodulin-binding site
    David S Libich
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    Biophys J 94:4847-66. 2008
    ....
  49. ncbi request reprint Dipolar chemical shift correlation spectroscopy for homonuclear carbon distance measurements in proteins in the solid state: application to structure determination and refinement
    Xiaohu Peng
    Department of Physics, Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
    J Am Chem Soc 130:359-69. 2008
    ..8 A. The approach is of general applicability for peptides and small proteins and can be easily incorporated into a structure determination and refinement protocol...
  50. ncbi request reprint Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza
    Jutta Nesper
    Department of Microbiology, University of Guelph, Guelph, Ontario N1G2W1, Canada
    J Biol Chem 278:49763-72. 2003
    ..Chemical cross-linking of intact cells suggested formation of a transmembrane complex minimally containing Wza and the inner membrane tyrosine autokinase Wzc...
  51. ncbi request reprint Interactions of the 18.5 kDa isoform of myelin basic protein with Ca2+-calmodulin: in vitro studies using gel shift assays
    David S Libich
    Department of Molecular Biology and Genetics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    Mol Cell Biochem 241:45-52. 2002
    ..Our results demonstrated further that the interaction involved the C-terminal domain of MBP, again in a primarily 1:1 molar ratio with CaM, consistent with our identification of a CaM-binding motif at the C-terminus...
  52. doi request reprint Parameterization of the proline analogue Aze (azetidine-2-carboxylic acid) for molecular dynamics simulations and evaluation of its effect on homo-pentapeptide conformations
    Kyrylo Bessonov
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
    J Mol Graph Model 39:118-25. 2013
    ....
  53. doi request reprint Interactions of intrinsically disordered Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes - synergistic effects of lipid composition and temperature on secondary structure
    Luna N Rahman
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol 88:791-807. 2010
    ..Moreover, this induced folding is enhanced at low temperatures, lending credence to the hypothesis that dehydrins stabilize plant outer and organellar membranes in conditions of cold...
  54. ncbi request reprint Interactions of the 18.5-kDa isoform of myelin basic protein with Ca(2+)-calmodulin: in vitro studies using fluorescence microscopy and spectroscopy
    David S Libich
    Department of Molecular Biology and Genetics, University of Guelph, ON, Canada
    Biochem Cell Biol 80:395-406. 2002
    ..These values show a roughly 1000-fold lower affinity of MBP for CaM than other CaM-binding peptides, such as myristoylated alanine-rich C-kinase substrate, that are involved in signal transduction...
  55. ncbi request reprint The formation of helical tubular vesicles by binary monolayers containing a nickel-chelating lipid and phosphoinositides in the presence of basic polypeptides
    Noboru Ishiyama
    Biophysics Interdepartmental Group, Department of Molecular Biology and Genetics, University of Guelph, 50 Stone Road East, Ont, N1G 2W1, Guelph, Canada
    Chem Phys Lipids 114:103-11. 2002
    ..There, comparable helical tubular structures might represent a means for sequestration of these lipids into domains of high local concentration, perhaps in regions where the membrane is greatly curved...
  56. ncbi request reprint Molecular dynamics exposes alpha-helices in myelin basic protein
    Ian R Bates
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, Ontario, Canada
    J Mol Model 9:290-7. 2003
    ..The degree of molecular extension was greater in the deiminated species, and the alpha-helices were more transient. These structural disruptions may be operative in vivo during multiple sclerosis...
  57. ncbi request reprint Hemoglobin as a source of iron overload in multiple sclerosis: does multiple sclerosis share risk factors with vascular disorders?
    Vladimir V Bamm
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
    Cell Mol Life Sci 71:1789-98. 2014
    ..Moreover, such considerations could also shed light on and explain the higher susceptibility of MS patients to a wide range of cardiovascular disorders. ..
  58. ncbi request reprint Regulatory effect of the glial Golli-BG21 protein on the full-length murine small C-terminal domain phosphatase (SCP1, or Golli-interacting protein)
    Sergio Jaramillo-Tatis
    Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada
    Biochem Biophys Res Commun 447:633-7. 2014
    ..The successful production of an active, full-length mSCP1/GIP thus demonstrates a role for its N-terminus in regulation of phosphatase activity, in events such as the regulation of transcription in oligodendrocytes...
  59. ncbi request reprint White matter rafting--membrane microdomains in myelin
    Lillian S DeBruin
    Department of Molecular and Cellular Biology, and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, ON, Canada
    Neurochem Res 32:213-28. 2007
    ....
  60. pmc Characterization of the cell wall of the sheathed methanogen Methanospirillum hungatei GP1 as an S layer
    M Firtel
    Department of Microbiology, College of Biological Sciences, University of Guelph, Ontario, Canada
    J Bacteriol 175:7550-60. 1993
    ..The cell wall was composed of two nonglycosylated polypeptides (114 and 110 kDa). The cell wall resembled an archaeal S layer and may function in regulating the passage of small (< 10-kDa) sheath precursor proteins...
  61. ncbi request reprint Effects of the osmolyte trimethylamine-N-oxide on conformation, self-association, and two-dimensional crystallization of myelin basic protein
    Christopher M Hill
    Department of Molecular Biology and Genetics, University of Guelph, 50 Stone Road East, Guelph, Ont, Canada N1G 2W1
    J Struct Biol 139:13-26. 2002
    ..Thus, this protein has an inherently disordered nature, which mitigates strongly against its crystallization for high-resolution structure determination...
  62. ncbi request reprint Three-dimensional structure of myelin basic protein. II. Molecular modeling and considerations of predicted structures in multiple sclerosis
    R A Ridsdale
    Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 272:4269-75. 1997
    ..Such mechanisms could be operative in demyelinating diseases such as multiple sclerosis...
  63. ncbi request reprint Human proteolipid protein (PLP) mediates winding and adhesion of phospholipid membranes but prevents their fusion
    N Palaniyar
    Department of Molecular Biology and Genetics, The University of Guelph, 50 Stone Road East, Guelph, Ont N1G 2W1, Canada
    Biochim Biophys Acta 1415:85-100. 1998
    ..Hence, our data provide direct evidence for PLP's suspected function of membrane adhesion, and also suggest that PLP could potentially play a role in the formation of the myelin sheath...
  64. ncbi request reprint Topology of recombinant rat upstream binding factor
    R A Ridsdale
    Department of Molecular Biology and Genetics, University of Guelph, ON, Canada
    Biochem Cell Biol 76:649-55. 1998
    ..Distinct nodes were observable, of size and spacing consistent with being HMG boxes 3 and 4. The dimerisation domain seemed most probably to be located in the internal region of the structure...
  65. ncbi request reprint Formation of folds and vesicles by dipalmitoylphosphatidylcholine monolayers spread in excess
    R A Ridsdale
    Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
    J Membr Biol 180:21-32. 2001
    ..It is concluded that DPPC monolayers can form bilayers spontaneously in the absence of surfactant apoproteins, other proteins or agents...
  66. ncbi request reprint The effects of deimination of myelin basic protein on structures formed by its interaction with phosphoinositide-containing lipid monolayers
    N Ishiyama
    Department of Molecular Biology and Genetics, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada
    J Struct Biol 136:30-45. 2001
    ..Sequence comparisons with other actin- and phosphoinositide-binding proteins (vinculin, ActA, MARCKS) suggested that the carboxyl-terminal segment of MBP could form an amphipathic alpha helix and was the phosphoinositide binding site...
  67. ncbi request reprint Electron paramagnetic resonance spectroscopy and molecular modelling of the interaction of myelin basic protein (MBP) with calmodulin (CaM)-diversity and conformational adaptability of MBP CaM-targets
    Eugenia Polverini
    Istituto Nazionale per la Fisica della Materia, Dipartimento di Fisica, Universita di Parma, Parco Area delle Scienze 7 A, 43100 Parma, Italy
    J Struct Biol 148:353-69. 2004
    ..The results obtained confirmed the appropriate utilisation of simple residue substitutions to mimic the natural modifications, and demonstrated molecular mechanisms by which MBP-CaM interactions could be modulated in vivo...
  68. ncbi request reprint Backbone resonance assignments of the 18.5 kDa isoform of murine myelin basic protein (MBP)
    David S Libich
    J Biomol NMR 29:545-6. 2004
  69. ncbi request reprint Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity
    Noboru Ishiyama
    Department of Biochemistry and Department of Microbiology and Immunology, McGill University, Montreal, Quebec
    J Biol Chem 281:24489-95. 2006
    ..Guided by evidence from site-directed mutagenesis and computational simulations, a three-step reaction mechanism is proposed that involves Lys-133 functioning as both a catalytic acid and base...
  70. ncbi request reprint Effect of arginine loss in myelin basic protein, as occurs in its deiminated charge isoform, on mediation of actin polymerization and actin binding to a lipid membrane in vitro
    Joan M Boggs
    Division of Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
    Biochemistry 44:3524-34. 2005
    ..Thus, this posttranslational modification of MBP, which occurs early in life and is increased in multiple sclerosis, attenuates the ability of MBP to polymerize and bundle actin, and to bind it to a negatively charged membrane...
  71. ncbi request reprint Binding of the proline-rich segment of myelin basic protein to SH3 domains: spectroscopic, microarray, and modeling studies of ligand conformation and effects of posttranslational modifications
    Eugenia Polverini
    Dipartimento di Fisica and CNISM, Universita di Parma, V le Usberti, 7 A, 43100 Parma, Italy
    Biochemistry 47:267-82. 2008
    ....