Robert Ew Hancock

Summary

Affiliation: University of British Columbia
Country: Canada

Publications

  1. ncbi Therapeutic potential of host defense peptides in antibiotic-resistant infections
    Nicole J Afacan
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, Canada
    Curr Pharm Des 18:807-19. 2012
  2. pmc The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells
    Anastasia Nijnik
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    J Leukoc Biol 91:599-607. 2012
  3. pmc Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro
    Suki M Y Lee
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China
    Respir Res 11:147. 2010
  4. pmc Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity
    Kelly L Brown
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    J Transl Med 8:6. 2010
  5. pmc Curating the innate immunity interactome
    David J Lynn
    Animal and Bioscience Research Department, AGRIC, Teagasc, Grange, Dunsany, Co Meath, Ireland
    BMC Syst Biol 4:117. 2010
  6. pmc Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome
    Clara Amid
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    BMC Genomics 10:606. 2009
  7. pmc Modulation of interleukin-1β-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts
    Emily Turner-Brannen
    Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, 799 John Buhler Research Centre, 715 McDermot Avenue, Winnipeg, MB, R3E3P4, Canada
    Arthritis Res Ther 13:R129. 2011
  8. ncbi Function of pseudomonas porins in uptake and efflux
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3
    Annu Rev Microbiol 56:17-38. 2002
  9. ncbi Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics
    R E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, B C, Canada V6T 1Z3
    Curr Drug Targets Infect Disord 2:79-83. 2002
  10. ncbi The relationship between peptide structure and antibacterial activity
    Jon Paul S Powers
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, BC, Canada V6T 1Z3
    Peptides 24:1681-91. 2003

Collaborators

Detail Information

Publications109 found, 100 shown here

  1. ncbi Therapeutic potential of host defense peptides in antibiotic-resistant infections
    Nicole J Afacan
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, Canada
    Curr Pharm Des 18:807-19. 2012
    ..Challenges to their development as new therapeutics are also discussed...
  2. pmc The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells
    Anastasia Nijnik
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    J Leukoc Biol 91:599-607. 2012
    ..These findings elucidate the common molecular mechanisms mediating the chemokine induction activity of natural and synthetic cationic peptides in monocytic cells and identify SFKs as a potential target for modulating peptide responses...
  3. pmc Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro
    Suki M Y Lee
    Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China
    Respir Res 11:147. 2010
    ..However a comprehensive gene expression profile of pdmH1N1 in relevant primary human cells in vitro has not been reported. Type I alveolar epithelial cells are a key target cell in pdmH1N1 pneumonia...
  4. pmc Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity
    Kelly L Brown
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    J Transl Med 8:6. 2010
    ..Many TLRs utilize a common signalling pathway that relies on activation of the kinase IRAK4 and the transcription factor NFkappaB for the rapid expression of immunity genes...
  5. pmc Curating the innate immunity interactome
    David J Lynn
    Animal and Bioscience Research Department, AGRIC, Teagasc, Grange, Dunsany, Co Meath, Ireland
    BMC Syst Biol 4:117. 2010
    ..InnateDB (http://www.innatedb.com) is a molecular interaction and pathway database developed to facilitate systems-level analyses of innate immunity...
  6. pmc Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome
    Clara Amid
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    BMC Genomics 10:606. 2009
    ..Defensins have been studied for over two decades and their naming has become a critical issue due to incorrect identification of defensin genes derived from different mouse strains and the duplicated nature of this region...
  7. pmc Modulation of interleukin-1β-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts
    Emily Turner-Brannen
    Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, 799 John Buhler Research Centre, 715 McDermot Avenue, Winnipeg, MB, R3E3P4, Canada
    Arthritis Res Ther 13:R129. 2011
    ..This study examined the impact of a 12-amino acid IDR peptide, IDR-1002, in pro-inflammatory cytokine interleukin (IL)-1β-induced responses in synovial fibroblasts, a critical cell type in the pathogenesis of inflammatory arthritis...
  8. ncbi Function of pseudomonas porins in uptake and efflux
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3
    Annu Rev Microbiol 56:17-38. 2002
    ..These familial relationships underlie functional similarities such that well-studied members of these families become prototypes for other members. We summarize here the latest information on these porins...
  9. ncbi Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics
    R E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, B C, Canada V6T 1Z3
    Curr Drug Targets Infect Disord 2:79-83. 2002
    ..A few of these peptide antibiotics have entered clinical trials to date, with mixed success. However, their diverse portfolio of structures, activity spectra, biological activities, and modes of action, provide substantial potential...
  10. ncbi The relationship between peptide structure and antibacterial activity
    Jon Paul S Powers
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, BC, Canada V6T 1Z3
    Peptides 24:1681-91. 2003
    ..In addition to peptide conformation, the role of membrane lipid composition, specifically non-bilayer lipids, on peptide activity will also be discussed...
  11. ncbi On the mechanism of solute uptake in Pseudomonas
    Sandeep Tamber
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, BC, Canada V6T 1Z3
    Front Biosci 8:s472-83. 2003
    ..The recent advances in elucidating the structures and functional mechanisms of these uptake systems will be discussed in this review...
  12. ncbi Concerns regarding resistance to self-proteins
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Microbiology 149:3343-4; discussion 3344-5. 2003
  13. ncbi Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies
    Robert E W Hancock
    Centre for Microbial Diseases and Immunity Research, Room 232, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4
    Nat Biotechnol 24:1551-7. 2006
    ....
  14. ncbi Mechanisms of action of newer antibiotics for Gram-positive pathogens
    Robert Ew Hancock
    University of British Columbia, Vancouver, British Columbia, Canada
    Lancet Infect Dis 5:209-18. 2005
    ..This review discusses the mechanisms of antibiotic action against Gram-positive pathogens, and resistance counter-mechanisms developed by Gram-positive bacteria, with emphasis on the newer agents...
  15. ncbi Host defence peptides from invertebrates--emerging antimicrobial strategies
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, 2259 Lower Mall, Vancouver, BC, Canada V6T 1Z4
    Immunobiology 211:315-22. 2006
    ..This enormous diversity is providing templates for the design and development of both antibiotic peptides and peptides that selectively modulate innate immunity to increase protection against infections and sepsis...
  16. ncbi Cationic peptides: effectors in innate immunity and novel antimicrobials
    R E Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Lancet Infect Dis 1:156-64. 2001
    ..One such function might involve the dampening of signalling by bacterial molecules such as lipopolysaccharide and lipoteichoic acid...
  17. ncbi Host defence (cationic) peptides: what is their future clinical potential?
    R E Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Drugs 57:469-73. 1999
    ..For this reason such peptides have entered clinical trials, largely as agents for topical therapy of polymicrobial infections and are considered to have excellent potential for being a novel antibiotic class...
  18. ncbi Role of membranes in the activities of antimicrobial cationic peptides
    Robert E W Hancock
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Blvd, Vancouver, BC, Canada V6T 1Z3
    FEMS Microbiol Lett 206:143-9. 2002
    ..Although dogma suggests that their lethal action involves disruption of the cytoplasmic membranes, a number of cationic peptides can traverse intact membranes to interact with internal targets...
  19. pmc Role of intracellular proteases in the antibiotic resistance, motility, and biofilm formation of Pseudomonas aeruginosa
    Lucía Fernández
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada
    Antimicrob Agents Chemother 56:1128-32. 2012
    ....
  20. pmc The role of antimicrobial peptides in animal defenses
    R E Hancock
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Proc Natl Acad Sci U S A 97:8856-61. 2000
    ..We are currently developing variant peptides as therapeutics against antibiotic-resistant infections...
  21. ncbi The role of cationic antimicrobial peptides in innate host defences
    R E Hancock
    Dept of Microbiology and Immunology, University of British Columbia, 300 6174 University Blvd, Vancouver, Canada V6T 1Z3
    Trends Microbiol 8:402-10. 2000
    ..In addition, they interact directly with host cells to modulate the inflammatory process and innate defences...
  22. ncbi The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility
    Alexandra K Marr
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, British Columbia V6T 1Z4, Canada
    Microbiology 153:474-82. 2007
    ..Studies with a lon mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa...
  23. pmc Role of lon, an ATP-dependent protease homolog, in resistance of Pseudomonas aeruginosa to ciprofloxacin
    Michelle D Brazas
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    Antimicrob Agents Chemother 51:4276-83. 2007
    ..The data are consistent with the hypothesis that the induction of Lon by ciprofloxacin is involved in adaptive resistance...
  24. pmc Ciprofloxacin induction of a susceptibility determinant in Pseudomonas aeruginosa
    Michelle D Brazas
    Centre for Microbial Diseases and Immunity Research, Room 232, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia, Canada
    Antimicrob Agents Chemother 49:3222-7. 2005
    ..C. Wolfgang et al., Proc. Natl. Acad. Sci. USA 100:8484-8489, 2003), these findings demonstrate that the R2/F2 pyocin region is a "loaded gun" that can mediate fluoroquinolone susceptibility in P. aeruginosa...
  25. pmc Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa
    W James Gooderham
    Center for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, 232 2259 Lower Mall Research Station, Vancouver, BC, Canada
    J Bacteriol 190:5624-34. 2008
    ..This suggests that PsrA might be a key regulator of antimicrobial peptide resistance and virulence...
  26. ncbi Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa
    Joseph B McPhee
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Blvd Vancouver BC, V6T 1Z3, Canada
    Mol Microbiol 50:205-17. 2003
    ..aeruginosa infections...
  27. pmc Adaptive resistance to the "last hope" antibiotics polymyxin B and colistin in Pseudomonas aeruginosa is mediated by the novel two-component regulatory system ParR-ParS
    Lucía Fernández
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, 232 2259 Lower Mall Research Station, Vancouver, BC, Canada V6T 1Z4
    Antimicrob Agents Chemother 54:3372-82. 2010
    ..This study highlights the complexity of the regulatory network controlling resistance to cationic antibiotics and host peptides in P. aeruginosa, which has major relevance in the development and deployment of cationic antimicrobials...
  28. pmc Human host defense peptide LL-37 prevents bacterial biofilm formation
    Joerg Overhage
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, Canada
    Infect Immun 76:4176-82. 2008
    ....
  29. doi The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa
    W James Gooderham
    Department of Microbiology and Immunology and Centre for Microbial Diseases and Immunity Research, University of British Columbia, 232 2259 Lower Mall, Vancouver, BC V6T 1Z4, Canada
    Microbiology 155:699-711. 2009
    ..It was also demonstrated that PhoQ controls the expression of many genes outside the known PhoP regulon...
  30. pmc Mechanism of action and limited cross-resistance of new lipopeptide MX-2401
    E Rubinchik
    BioWest Therapeutics Inc, Suite 400, 1727 West Broadway, Vancouver, British Columbia V6J4W61, Canada
    Antimicrob Agents Chemother 55:2743-54. 2011
    ..Overall, these results provided strong evidence that the mode of action of MX-2401 is unique and different from that of any of the approved antibiotics, including daptomycin...
  31. ncbi Sequence requirements and an optimization strategy for short antimicrobial peptides
    Kai Hilpert
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z3, Canada
    Chem Biol 13:1101-7. 2006
    ..This process provides a cost and time effective enrichment of new candidates for drug development, increasing the chances of finding pharmacologically relevant peptides...
  32. ncbi Design of host defence peptides for antimicrobial and immunity enhancing activities
    Joseph B McPhee
    Department of Microbiology and Immunology, University of British Columbia, Lower Mall Research Station, 232 2259 Lower Mall, Vancouver BC, V6T 1Z4 Canada
    Comb Chem High Throughput Screen 8:257-72. 2005
    ..We also review recent literature on what structural components are related to these immunomodulatory effects. It must be stressed however that these studies, and the area of peptide research, are still in their infancy...
  33. pmc Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered susceptibility
    Elena B M Breidenstein
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Room 232, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T1Z4, Canada
    Antimicrob Agents Chemother 52:4486-91. 2008
    ..Thirty-five and 79 mutants with increased and decreased susceptibilities, respectively, were identified, as confirmed by broth dilution...
  34. ncbi Alternative mechanisms of action of cationic antimicrobial peptides on bacteria
    John D F Hale
    University of British Columbia, Centre for Microbial Disease and Immunity Research, Department of Microbiology and Immunology, Lower Mall Research Station, Vancouver, BC V6T1Z4, Canada
    Expert Rev Anti Infect Ther 5:951-9. 2007
    ..This article presents an updated review of how cationic antimicrobial peptides are able to affect bacterial killing, with a focus on internal targets...
  35. pmc Novel genetic determinants of low-level aminoglycoside resistance in Pseudomonas aeruginosa
    Kristen N Schurek
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Room 232, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T1Z4, Canada
    Antimicrob Agents Chemother 52:4213-9. 2008
    ..The results of this study emphasize the complexity of the interactions that tobramycin may have within the bacterial cell and introduce a large number of novel genes which may play a role in tobramycin resistance...
  36. pmc Construction of a mini-Tn5-luxCDABE mutant library in Pseudomonas aeruginosa PAO1: a tool for identifying differentially regulated genes
    Shawn Lewenza
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    Genome Res 15:583-9. 2005
    ..This dual-purpose mutant library allows for functional and regulation studies and will serve as a resource for the research community to further our understanding of P. aeruginosa biology...
  37. pmc Swarming of Pseudomonas aeruginosa is a complex adaptation leading to increased production of virulence factors and antibiotic resistance
    Joerg Overhage
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, BC, Canada
    J Bacteriol 190:2671-9. 2008
    ..These results clearly favored the conclusion that swarming of P. aeruginosa is a complex adaptation process in response to a viscous environment resulting in a substantial change in virulence gene expression and antibiotic resistance...
  38. ncbi QSAR modeling and computer-aided design of antimicrobial peptides
    Håvard Jenssen
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
    J Pept Sci 14:110-4. 2008
    ....
  39. doi Inhibition of HSV cell-to-cell spread by lactoferrin and lactoferricin
    Håvard Jenssen
    Department of Medical Microbiology, University Hospital of North Norway, N 9038 Tromsø, Norway
    Antiviral Res 79:192-8. 2008
    ..In contrast, the cellular localization of iron-saturated (holo)-Lf appeared to differ from that of apo-Lf, indicating that holo- and apo-Lf may exhibit different antiviral mechanisms...
  40. ncbi An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane
    Trevor F Moraes
    Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Nat Struct Mol Biol 14:85-7. 2007
    ..Lysine residues coat the inner periplasmic surface, creating an 'electropositive sink' that pulls the phosphates through the eyelet and into the cell...
  41. pmc Sublethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli
    Aleksander Patrzykat
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    Antimicrob Agents Chemother 46:605-14. 2002
    ..Better understanding of the effects of peptides acting at their MICs will contribute to the design of new peptides effective at lower, less toxic concentrations...
  42. doi Regulation of virulence and antibiotic resistance by two-component regulatory systems in Pseudomonas aeruginosa
    W James Gooderham
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
    FEMS Microbiol Rev 33:279-94. 2009
    ..Here, we summarize the current body of knowledge of these and other two-component systems that provides insight into the complex regulation of virulence and resistance in P. aeruginosa...
  43. pmc Involvement of pmrAB and phoPQ in polymyxin B adaptation and inducible resistance in non-cystic fibrosis clinical isolates of Pseudomonas aeruginosa
    Kristen N Schurek
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, British Columbia, Canada
    Antimicrob Agents Chemother 53:4345-51. 2009
    ..The results presented here suggest that the skipped-well isolates have the ability to adapt to specific concentrations of polymyxin B, inducing known polymyxin B resistance genes involved in generating alterations in the outer membrane...
  44. pmc Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR
    Amy T Y Yeung
    Center for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, BC, Canada
    J Bacteriol 191:5592-602. 2009
    ..The observed dysregulation in the metR mutant of nine different genes required for swarming motility provided a possible explanation for the swarming-deficient phenotype of this mutant...
  45. pmc Multidrug efflux systems play an important role in the invasiveness of Pseudomonas aeruginosa
    Yoichi Hirakata
    Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, V5Z 4H4 Canada
    J Exp Med 196:109-18. 2002
    ..We conclude that the P. aeruginosa MexAB-OprM efflux system exports virulence determinants that contribute to bacterial virulence...
  46. pmc Synergistic interactions between mammalian antimicrobial defense peptides
    H Yan
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    Antimicrob Agents Chemother 45:1558-60. 2001
    ..We demonstrate that mammalian peptides from different structural classes frequently show synergy with each other and selectively show synergy with human lysozyme...
  47. pmc Peptide antimicrobial agents
    Håvard Jenssen
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Lower Mall Research Station, 232 2259 Lower Mall, Vancouver, British Columbia V6T 1Z4, Canada
    Clin Microbiol Rev 19:491-511. 2006
    ..Knowledge regarding the relationship between peptide structure and function as well as their mechanism of action is being applied in the design of antimicrobial peptide variants as potential novel therapeutic agents...
  48. pmc Characterization of OpdH, a Pseudomonas aeruginosa porin involved in the uptake of tricarboxylates
    Sandeep Tamber
    Department of Microbiology and Immunology, University of British Columbia, 235 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z4 Canada
    J Bacteriol 189:929-39. 2007
    ..Thus, these data suggest that the requirement for OpdH for efficient growth on tricarboxylates was likely due to the specific expression of this large-channel porin under particular growth conditions...
  49. pmc Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant library
    Joerg Overhage
    Centre for Microbial Diseases and Immunity, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    J Bacteriol 189:2164-9. 2007
    ..aeruginosa. Conversely, many of the swarming-negative mutants also showed an impairment in biofilm formation, indicating a strong relationship between these types of growth states...
  50. pmc Aminoglycoside efflux in Pseudomonas aeruginosa: involvement of novel outer membrane proteins
    James T H Jo
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3
    Antimicrob Agents Chemother 47:1101-11. 2003
    ..Based on the data, we propose that OpmG is a major outer membrane efflux channel involved in aminoglycoside efflux in P. aeruginosa PAK and that OpmI, its most related paralog, may share an overlapping function...
  51. pmc Effect of membrane composition on antimicrobial peptides aurein 2.2 and 2.3 from Australian southern bell frogs
    John T J Cheng
    Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada
    Biophys J 96:552-65. 2009
    ..3-COOH was the least efficient. Taken together, these data show that the membrane interactions of aurein peptides are affected by the hydrophobic thickness of the lipid bilayers and the PG content...
  52. doi Antimicrobial properties of lactoferrin
    Håvard Jenssen
    Centre for Microbial Diseases and Immunity Research, 232 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
    Biochimie 91:19-29. 2009
    ..In this review, we focus on the antimicrobial activities of lactoferrin with particular emphasis on antibacterial and antiviral activities, although its antifungal and -parasitic activity are also discussed...
  53. ncbi Using microarray gene signatures to elucidate mechanisms of antibiotic action and resistance
    Michelle D Brazas
    Centre for Microbial Diseases and Immunity Research, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, Canada, V6T 1Z4
    Drug Discov Today 10:1245-52. 2005
    ..A more detailed knowledge of how known antibiotics act should reveal new strategies for antimicrobial drug discovery...
  54. doi Identification of novel antibacterial peptides by chemoinformatics and machine learning
    Christopher D Fjell
    Department of Medicine, Division of Infectious Diseases, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    J Med Chem 52:2006-15. 2009
    ....
  55. doi Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic-resistant superbugs
    Artem Cherkasov
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia V6T 1Z3, Canada
    ACS Chem Biol 4:65-74. 2009
    ....
  56. doi Screening and characterization of surface-tethered cationic peptides for antimicrobial activity
    Kai Hilpert
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada
    Chem Biol 16:58-69. 2009
    ..Structure-activity modeling of the structural features determining the activity of tethered peptides indicated that the extent and positioning of positive charges and hydrophobic residues were influential in determining activity...
  57. ncbi Antibacterial peptides for therapeutic use: obstacles and realistic outlook
    Alexandra K Marr
    Department of Microbiology and Immunology, and Centre for Microbial Diseases and Immunity Research, University of British Columbia, 232 2259 Lower Mall, Vancouver, BC V6T 1Z4, Canada
    Curr Opin Pharmacol 6:468-72. 2006
    ..Thus, there is hope that they will spawn a new generation of antimicrobials with a broad range of topical and systemic applications against infections...
  58. ncbi Structure of the bovine antimicrobial peptide indolicidin bound to dodecylphosphocholine and sodium dodecyl sulfate micelles
    A Rozek
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    Biochemistry 39:15765-74. 2000
    ..The preferred location of indolicidin in DPC micelles and lipid bilayers, analyzed using spin-label probes, was at the membrane interface...
  59. ncbi Interaction of cationic antimicrobial peptides with model membranes
    L Zhang
    Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 276:35714-22. 2001
    ..Overall, whereas all studied cationic antimicrobial peptides interacted with membranes, they were quite heterogeneous in their impact on these membranes...
  60. doi Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
    Irith Wiegand
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z4, Canada
    Nat Protoc 3:163-75. 2008
    ..Growth is assessed after incubation for a defined period of time (16-20 h) and the MIC value is read. This protocol applies only to aerobic bacteria and can be completed in 3 d...
  61. pmc Mutator genes giving rise to decreased antibiotic susceptibility in Pseudomonas aeruginosa
    Irith Wiegand
    Centre for Microbial Diseases and Immunity Research, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia, Canada
    Antimicrob Agents Chemother 52:3810-3. 2008
    ..aeruginosa, including PA3958 and RadA (PA4609)...
  62. pmc The commensal Streptococcus salivarius K12 downregulates the innate immune responses of human epithelial cells and promotes host-microbe homeostasis
    Celine Cosseau
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, Canada
    Infect Immun 76:4163-75. 2008
    ..S. salivarius K12 might thereby ensure that it is tolerated by the host and maintained on the epithelial surface while actively protecting the host from inflammation and apoptosis induced by pathogens...
  63. pmc Metabolic changes associated with adaptive diversification in Escherichia coli
    Mickael Le Gac
    Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Genetics 178:1049-60. 2008
    ....
  64. ncbi Evaluating different descriptors for model design of antimicrobial peptides with enhanced activity toward P. aeruginosa
    Håvard Jenssen
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
    Chem Biol Drug Des 70:134-42. 2007
    ..7 +/- 1.6 peptides. The model has also been proven significantly more accurate than a simpler model (Bac2a- #1), where the inductive and conventional quantitative structure-activity relationship descriptors were excluded...
  65. ncbi Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology
    Kai Hilpert
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, no 2259 Lower Mall Research Station, Vancouver, British Columbia, V6T 1Z3, Canada
    Nat Protoc 2:1652-60. 2007
    ..The protocol can be completed in 3 d...
  66. ncbi Structure-based design of an indolicidin peptide analogue with increased protease stability
    Annett Rozek
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, British Columbia, Canada
    Biochemistry 42:14130-8. 2003
    ..These findings suggest that cyclization may serve as an important strategy in the rational design of antimicrobial peptides...
  67. ncbi Structure-activity relationships for the beta-hairpin cationic antimicrobial peptide polyphemusin I
    Jon Paul S Powers
    Department of Microbiology and Immunology, University of British Columbia, 300 6174 University Boulevard, Vancouver, British Columbia, V6T 1Z3, Canada
    Biochim Biophys Acta 1698:239-50. 2004
    ..Disruption of this structure results in reduced antimicrobial activity and completely abolishes membrane translocation indicating that the linear PM1-S acts through a different antimicrobial mechanism...
  68. pmc ProbeLynx: a tool for updating the association of microarray probes to genes
    Fiona M Roche
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada
    Nucleic Acids Res 32:W471-4. 2004
    ..By also including the latest gene function annotation information in the output, ProbeLynx provides the critical first step in updating microarray data annotation...
  69. ncbi The cationic antimicrobial peptide LL-37 modulates dendritic cell differentiation and dendritic cell-induced T cell polarization
    Donald J Davidson
    British Columbia Research Institute for Child and Family Health, Room 381, 950 West 28th Avenue, Vancouver, British Columbia, Canada V5Z 4H4
    J Immunol 172:1146-56. 2004
    ..LL-37 may be an attractive therapeutic candidate for manipulating T cell polarization by DC...
  70. ncbi Salmonella's sensor for host defense molecules
    Robert E W Hancock
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
    Cell 122:320-2. 2005
    ..2005) decipher an elegant mechanism by which the PhoQ sensor kinase of Salmonella is switched on by host cationic antimicrobial peptides, leading to changes in gene expression that enable Salmonella to combat the host immune response...
  71. ncbi Cationic host defense (antimicrobial) peptides
    Kelly L Brown
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
    Curr Opin Immunol 18:24-30. 2006
    ..In addition, the mechanisms of action are being unraveled, which support more effective implementation of derivatives of these endogenous peptides as therapeutic agents...
  72. pmc Apoptosis of airway epithelial cells: human serum sensitive induction by the cathelicidin LL-37
    Y Elaine Lau
    Centre for Microbial Diseases and Immunity Research, Room 232, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, BC V6T 1Z4 Canada
    Am J Respir Cell Mol Biol 34:399-409. 2006
    ..We propose that LL-37-induced apoptosis of epithelial cells at low serum tissue sites may have a protective role against bacterial infection...
  73. pmc Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa
    Sandeep Tamber
    Department of Microbiology and Immunology, University of British Columbia, No 235 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T 1Z4, Canada
    J Bacteriol 188:45-54. 2006
    ..These results imply that there is a functional basis for the phylogenetic clustering of these proteins and provide a framework for studying OprD homologues in other organisms...
  74. ncbi High-throughput generation of small antibacterial peptides with improved activity
    Kai Hilpert
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    Nat Biotechnol 23:1008-12. 2005
    ..5 microg/ml against Escherichia coli. Similarly, we generated an 8-mer substituted peptide that showed broad spectrum activity, with an MIC of 2 microg/ml, against E. coli and Staphylococcus aureus...
  75. pmc Characterization of the structure and membrane interaction of the antimicrobial peptides aurein 2.2 and 2.3 from Australian southern bell frogs
    Yeang Ling Pan
    Department of Chemistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada
    Biophys J 92:2854-64. 2007
    ..The correlation between structure, membrane interaction, and activity are discussed in light of these results...
  76. ncbi Involvement of two related porins, OprD and OpdP, in the uptake of arginine by Pseudomonas aeruginosa
    Sandeep Tamber
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
    FEMS Microbiol Lett 260:23-9. 2006
    ..The impact of this apparent functional redundancy on the genetic fitness of P. aeruginosa is discussed...
  77. ncbi The bacterial outer membrane as a drug barrier
    R E Hancock
    Dept of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Trends Microbiol 5:37-42. 1997
    ..Restricted outer membrane permeability works in synergy with co-determinant resistance mechanisms, such as the periplasmic enzyme beta-lactamase or active efflux mechanisms, bringing about antibiotic resistance...
  78. pmc Synergy of histone-derived peptides of coho salmon with lysozyme and flounder pleurocidin
    A Patrzykat
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    Antimicrob Agents Chemother 45:1337-42. 2001
    ....
  79. doi Potential of immunomodulatory host defense peptides as novel anti-infectives
    Donna M Easton
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, Room 232, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, BC Canada V6T 1Z4
    Trends Biotechnol 27:582-90. 2009
    ..IDRs show significant promise and current research is uncovering mechanistic information that will aid in the future development of IDRs for clinical use...
  80. doi The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel
    Joseph B McPhee
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, Lower Mall Research Station, Vancouver, BC, Canada
    FEMS Microbiol Lett 296:241-7. 2009
    ....
  81. doi Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment
    Anastasia Nijnik
    Department of Microbiology and Immunology, Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, British Columbia, Canada
    J Immunol 184:2539-50. 2010
    ....
  82. doi Identification of novel host defense peptides and the absence of alpha-defensins in the bovine genome
    Christopher D Fjell
    Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    Proteins 73:420-30. 2008
    ..Since we could find no technical reasons these would be missed and no references to bovine alpha-defensins in the literature, this suggests that cattle lack this important family of host defense peptides...
  83. ncbi An anti-infective peptide that selectively modulates the innate immune response
    Monisha G Scott
    Inimex Pharmaceuticals Inc, 3650 Wesbrook Mall, Vancouver, British Columbia, Canada V6S 2L2
    Nat Biotechnol 25:465-72. 2007
    ..To our knowledge, an innate defense regulator that counters infection by selective modulation of innate immunity without obvious toxicities has not been reported previously...
  84. doi New insights into cathelicidin modulation of adaptive immunity
    Kelli Wuerth
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    Eur J Immunol 41:2817-9. 2011
    ....
  85. pmc Antimicrobial properties of MX-2401, an expanded-spectrum lipopeptide active in the presence of lung surfactant
    Dominique Dugourd
    BioWest Therapeutics Inc, Suite 1320, 885 West Georgia, Vancouver, British Columbia, Canada V6C 2G2
    Antimicrob Agents Chemother 55:3720-8. 2011
    ..In conclusion, MX-2401 is a promising new-generation lipopeptide for the treatment of serious infections with Gram-positive bacteria, including hospital-acquired pneumonia...
  86. pmc The pmrCAB operon mediates polymyxin resistance in Acinetobacter baumannii ATCC 17978 and clinical isolates through phosphoethanolamine modification of lipid A
    Luis A Arroyo
    Centre for Microbial Diseases and Immunity Research, 232 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Antimicrob Agents Chemother 55:3743-51. 2011
    ..These results demonstrate that specific alterations in the sequence of the pmrCAB operon are responsible for resistance to polymyxins in A. baumannii...
  87. pmc The sensor kinase CbrA is a global regulator that modulates metabolism, virulence, and antibiotic resistance in Pseudomonas aeruginosa
    Amy T Y Yeung
    University of British Columbia, Vancouver, BC, Canada
    J Bacteriol 193:918-31. 2011
    ..However, as CbrB did not have a resistance phenotype, CbrA likely modulates antibiotic resistance in a manner independent of CbrB...
  88. pmc Involvement of an ATP-dependent protease, PA0779/AsrA, in inducing heat shock in response to tobramycin in Pseudomonas aeruginosa
    Kristen N Kindrachuk
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, No 232, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T 1Z4, Canada
    Antimicrob Agents Chemother 55:1874-82. 2011
    ....
  89. ncbi Structure and mechanism of action of an indolicidin peptide derivative with improved activity against gram-positive bacteria
    C L Friedrich
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 276:24015-22. 2001
    ..These studies have introduced a cationic peptide with a unique structure and an ability to interact with membranes and to affect intracellular synthesis of proteins, RNA, and DNA...
  90. ncbi Mapping and characterization of two mutations to antibiotic supersusceptibility in Pseudomonas aeruginosa
    B L Angus
    Department of Microbiology, University of British Columbia, Vancouver, Canada
    J Gen Microbiol 133:2905-14. 1987
    ..The absB mutation caused a structurally undefined alteration in the physical interaction of EDTA and gentamicin with the outer membrane...
  91. ncbi Antimicrobial constituents of Rhus glabra
    G Saxena
    Department of Botany, University of British Columbia, Vancouver, Canada
    J Ethnopharmacol 42:95-9. 1994
    ..The first two compounds are reported here for the first time from Rhus glabra. Their structures were established using spectroscopic and chemical methods...
  92. pmc Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression
    X Liao
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    J Bacteriol 179:1490-6. 1997
    ..3H]penicillin-binding assays indicated that PBP3 was mainly produced during exponential growth whereas PBP3x was produced in the stationary phase of growth...
  93. ncbi PhyloBLAST: facilitating phylogenetic analysis of BLAST results
    F S Brinkman
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3
    Bioinformatics 17:385-7. 2001
    ..Flexible features such as ability to input your own multiple sequence alignment and use PHYLIP program options provide additional web-based phylogenetic analysis functionality beyond the analysis of a BLAST result...
  94. pmc Fluoroquinolone supersusceptibility mediated by outer membrane protein OprH overexpression in Pseudomonas aeruginosa: evidence for involvement of a nonporin pathway
    M Young
    Department of Microbiology, University of British Columbia, Vancouver, Canada
    Antimicrob Agents Chemother 36:2365-9. 1992
    ..This suggests that OprH is not a porin but, instead, may cause increased uptake of quinolones and chloramphenicol via a non-porin pathway...
  95. ncbi Optimization of microbial specificity in cyclic peptides by modulation of hydrophobicity within a defined structural framework
    Leslie H Kondejewski
    Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 277:67-74. 2002
    ..Our results show that the balance between activity and specificity in the present cyclic peptides can be optimized for each microorganism by systematic modulation of hydrophobicity...
  96. ncbi Membrane binding and permeation by indolicidin analogs studied by a biomimetic lipid/polydiacetylene vesicle assay
    Revital Halevy
    Department of Chemistry and Stadler Minerva Center for Mesoscopic Macromolecular Engineering, Ben Gurion University of the Negev, Beersheva 84105, Israel
    Peptides 24:1753-61. 2003
    ..The degree of hemolysis induced by the analogs, on the other hand, correlated to the extent of penetration into the hydrophobic core of the lipid assembly...
  97. pmc Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane
    María A Llamas
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    J Bacteriol 185:4707-16. 2003
    ..These results suggest that the Tol-OprL system is necessary for appropriate functioning of certain uptake systems at the level of the cytoplasmic membrane...
  98. pmc The complexities of antibiotic action
    Robert E W Hancock
    Mol Syst Biol 3:142. 2007
  99. ncbi Effect of divalent cations on the structure of the antibiotic daptomycin
    Steven W Ho
    Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada
    Eur Biophys J 37:421-33. 2008
    ..Similar studies on the divalent cation Mg(2+) are also presented. The implication of these results for the biological function of daptomycin is discussed...
  100. pmc Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index
    Yuxin Chen
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Aurora, Colorado 80045, USA
    J Biol Chem 280:12316-29. 2005
    ....
  101. pmc Effects of net charge and the number of positively charged residues on the biological activity of amphipathic alpha-helical cationic antimicrobial peptides
    Ziqing Jiang
    Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
    Biopolymers 90:369-83. 2008
    ..e., the change from +8 to +9 resulted in greater than 32-fold increase in hemolytic activity (250 microg/ml to <7.8 microg/ml, respectively)...