ROY GRAVEL

Summary

Affiliation: University of Calgary
Country: Canada

Publications

  1. pmc Thermolability of mutant MMACHC protein in the vitamin B12-responsive cblC disorder
    D S Froese
    Department of Biochemistry and Molecular Biology, University of Calgary, Alberta Children s Hospital Research Institute for Child and Maternal Health, Calgary, Alta, Canada
    Mol Genet Metab 100:29-36. 2010
  2. ncbi request reprint Molecular genetics of biotin metabolism: old vitamin, new science
    Roy A Gravel
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    J Nutr Biochem 16:428-31. 2005
  3. pmc Nonenzymatic biotinylation of histone H2A
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada
    Protein Sci 18:314-28. 2009
  4. doi request reprint Ligand-binding by catalytically inactive mutants of the cblB complementation group defective in human ATP:cob(I)alamin adenosyltransferase
    Jun Zhang
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Mol Genet Metab 98:278-84. 2009
  5. doi request reprint Structural impact of human and Escherichia coli biotin carboxyl carrier proteins on biotin attachment
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Biochemistry 49:4687-94. 2010
  6. ncbi request reprint Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Hum Mol Genet 11:3361-9. 2002
  7. ncbi request reprint Reduced histone biotinylation in multiple carboxylase deficiency patients: a nuclear role for holocarboxylase synthetase
    Monica A Narang
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Hum Mol Genet 13:15-23. 2004
  8. ncbi request reprint Impact of cblB mutations on the function of ATP:cob(I)alamin adenosyltransferase in disorders of vitamin B12 metabolism
    Jun Zhang
    Department of Biochemistry and Molecular Biology, University of Calgary, Heritage Medical Research Building, AB, Canada
    Mol Genet Metab 87:315-22. 2006
  9. doi request reprint Biotin is not a natural histone modification
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Biochim Biophys Acta 1789:719-33. 2009
  10. ncbi request reprint Homozygous nonsense mutation in the MCEE gene and siRNA suppression of methylmalonyl-CoA epimerase expression: a novel cause of mild methylmalonic aciduria
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Mol Genet Metab 88:327-33. 2006

Research Grants

Collaborators

  • Shannon Healy
  • Jun Zhang
  • Nicola Longo
  • J R Davie
  • U C Oppermann
  • Alfonso León-Del-Río
  • D Watkins
  • C Melissa Dobson
  • D S Froese
  • David S Rosenblatt
  • Daniel Leclerc
  • Monica A Narang
  • Diana Pacheco-Alvarez
  • Xuchu Wu
  • Timothy Wai
  • G Kochan
  • M McDonald
  • F H Niesen
  • Caroline Belair
  • Jordan Lerner-Ellis
  • Abigail Gradinger
  • Melissa Lemieux
  • Richard Dumas
  • Rafael Cervantes-Roldán
  • Antonio Velazquez
  • R Sergio Solorzano-Vargas
  • Linda M Ayer
  • Monica Narang
  • Carole Dore
  • Jordan P Lerner-Ellis
  • Aaron Wilson
  • Hakan Kadir
  • Thomas J Hudson
  • Thomas Hudson

Detail Information

Publications12

  1. pmc Thermolability of mutant MMACHC protein in the vitamin B12-responsive cblC disorder
    D S Froese
    Department of Biochemistry and Molecular Biology, University of Calgary, Alberta Children s Hospital Research Institute for Child and Maternal Health, Calgary, Alta, Canada
    Mol Genet Metab 100:29-36. 2010
    ....
  2. ncbi request reprint Molecular genetics of biotin metabolism: old vitamin, new science
    Roy A Gravel
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    J Nutr Biochem 16:428-31. 2005
    ..It may change our outlook on the optimal nutritional intake of biotin and its importance in biological processes such as development, cellular homeostasis and regulation...
  3. pmc Nonenzymatic biotinylation of histone H2A
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada
    Protein Sci 18:314-28. 2009
    ..None of the lysine sites within H2A resembles the biotin attachment consensus sequence seen in carboxylases, suggesting a novel mechanism for histone biotinylation...
  4. doi request reprint Ligand-binding by catalytically inactive mutants of the cblB complementation group defective in human ATP:cob(I)alamin adenosyltransferase
    Jun Zhang
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Mol Genet Metab 98:278-84. 2009
    ..Characterization of ligand-binding by MMAB provides insight into the mechanism of cobalamin adenosylation and the effect of patient mutations in the inherited disorder...
  5. doi request reprint Structural impact of human and Escherichia coli biotin carboxyl carrier proteins on biotin attachment
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Biochemistry 49:4687-94. 2010
    ..While the function of the thumb loop remains unknown, these results indicate a constraint on specificity of the bacterial substrate for biotin attachment that is not itself a feature of BirA...
  6. ncbi request reprint Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Hum Mol Genet 11:3361-9. 2002
    ..These data confirm that the identified gene, MMAB, corresponds to the cblB complementation group and has the appearance of a cob(I)alamin adenosyltransferase, as predicted from biochemical data...
  7. ncbi request reprint Reduced histone biotinylation in multiple carboxylase deficiency patients: a nuclear role for holocarboxylase synthetase
    Monica A Narang
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Hum Mol Genet 13:15-23. 2004
    ....
  8. ncbi request reprint Impact of cblB mutations on the function of ATP:cob(I)alamin adenosyltransferase in disorders of vitamin B12 metabolism
    Jun Zhang
    Department of Biochemistry and Molecular Biology, University of Calgary, Heritage Medical Research Building, AB, Canada
    Mol Genet Metab 87:315-22. 2006
    ..Characterization of MMAB will contribute to our understanding of cobalamin processing in mammalian cells and of disease mechanisms in the genetic disorders...
  9. doi request reprint Biotin is not a natural histone modification
    Shannon Healy
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Biochim Biophys Acta 1789:719-33. 2009
    ..We conclude that biotin is absent in native histones to a sensitivity of at least one part per 100,000, suggesting that the regulatory impact of biotin on gene expression must be through alternate mechanisms...
  10. ncbi request reprint Homozygous nonsense mutation in the MCEE gene and siRNA suppression of methylmalonyl-CoA epimerase expression: a novel cause of mild methylmalonic aciduria
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, Canada
    Mol Genet Metab 88:327-33. 2006
    ..We conclude that the patient's MCEE defect was responsible for the mild methylmalonic aciduria, confirming a partial requirement for the enzymatic activity in humans...
  11. pmc Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, AB, Canada T2N 1N4
    Proc Natl Acad Sci U S A 99:15554-9. 2002
    ..We speculate that we have identified a component of a transporter or an accessory protein that is involved in the translocation of vitamin B(12) into mitochondria...
  12. ncbi request reprint Paradoxical regulation of biotin utilization in brain and liver and implications for inherited multiple carboxylase deficiency
    Diana Pacheco-Alvarez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico DF 04510, Mexico
    J Biol Chem 279:52312-8. 2004
    ....

Research Grants7

  1. GENETIC RISK FACTORS FOR HYPERHOMOCYSTEINEMIA
    ROY GRAVEL; Fiscal Year: 2000
    ..These studies, will determine the importance of genotype in the metabolic causes of hyperhomocysteinemia and risk of cardiovascular disease. (End of Abstract) ..
  2. GENETIC RISK FACTORS FOR HYPERHOMOCYSTEINEMIA
    ROY GRAVEL; Fiscal Year: 2006
    ..In summary, our study will give a biochemical and physiological face to the impact of 122M on enzyme function, development and disease. ..