Research Topics
Genomes and Genes | J N Mark GloverSummaryAffiliation: University of Alberta Country: Canada Publications
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Publications
Insights into the molecular basis of human hereditary breast cancer from studies of the BRCA1 BRCT domainJ N Mark Glover
Department of Biochemistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada
Fam Cancer 5:89-93. 2006..Missense variants identified in breast cancer screening programs often disrupt these interactions and these molecular defects may lead to an increased cancer risk...- Interactions between BRCT repeats and phosphoproteins: tangled up in twoJ N Mark Glover
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
Trends Biochem Sci 29:579-85. 2004.... - Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tailMegan S Lee
Department of Biochemistry, University of Alberta, Edmonton, Canada
J Biol Chem 280:32053-6. 2005.... 
Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substratesNicolas Coquelle
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
Proc Natl Acad Sci U S A 108:21022-7. 2011..A positively charged surface distinct from the active site specifically facilitates interactions with double-stranded substrates, providing a complex DNA binding surface that enables the recognition of diverse substrates...
Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1Stephen J Campbell
Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
Structure 18:167-76. 2010..A mutation in MDC1 that induces a more BRCA1-like conformation relaxes the binding specificity, allowing the mutant to bind phosphopeptides lacking a -COO(-) terminus...- Recognition of 5'-YpG-3' sequences by coupled stacking/hydrogen bonding interactions with amino acid residuesJason S Lamoureux
Department of Biochemistry, University of Alberta, Edmonton, Alta, Canada T6G 2H7
J Mol Biol 335:399-408. 2004..In most cases, this mode of DNA recognition helps explain the sequence specificity of the protein for its target DNA... - The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50Ross A Edwards
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochemistry 47:11446-56. 2008..BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage signals directly to RNA polymerase... - Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assaysMegan S Lee
Department of Biochemistry, School of Systems Molecular Medicine, University of Alberta, Edmonton, Alberta, Canada
Cancer Res 70:4880-90. 2010..The excellent agreement between the structure/function effects of these mutations and available clinical data supports the notion that functional and structure information can be useful in the development of models to assess cancer risk... - Structural basis for phosphorylation-dependent signaling in the DNA-damage responseR Scott Williams
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
Biochem Cell Biol 83:721-7. 2005..Our studies have revealed a flexible mode of recognition that allows PNK to interact with numerous negatively charged substrates... - The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinaseNina K Bernstein
Department of Biochemistry, 4 74 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Mol Cell 17:657-70. 2005..The FHA domain is linked to the kinase/phosphatase catalytic domain by a flexible tether, and it exhibits a mode of target selection based on electrostatic complementarity between the binding surface and the phosphothreonine peptide... - Insights from the crystal structure of the sixth BRCT domain of topoisomerase IIbeta binding protein 1Charles Chung Yun Leung
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Protein Sci 19:162-7. 2010..We further provide insight into poly(ADP-ribose) binding and sites of potential modification by PARP-1... - Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1R Scott Williams
Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
Nat Struct Mol Biol 11:519-25. 2004..The diminished peptide-binding capacity observed for cancer-associated BRCA1-BRCT variants may explain the enhanced cancer risks associated with these mutations... - N. meningitidis 1681 is a member of the FinO family of RNA chaperonesSteven Chaulk
Department of Biochemistry, University of Alberta, Edmonton, Canada
RNA Biol 7:812-9. 2010..This study strongly suggests that NMB1681 is a FinO-like RNA chaperone that likely regulates gene expression through RNA-based mechanisms in N. meningitidis... - Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugationJun Lu
Departments of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
Mol Microbiol 70:89-99. 2008.... - Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint controlCharles Chung Yun Leung
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 286:4292-301. 2011..Together with systematic mutagenesis studies, we highlight the role of key contacts in governing the unique specificity of the TopBP1-BACH1 interaction... - Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraMJoyce J W Wong
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, AB T6G 2H7, Canada
Nucleic Acids Res 39:6775-88. 2011.... - Detection of protein folding defects caused by BRCA1-BRCT truncation and missense mutationsR Scott Williams
Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 278:53007-16. 2003.... - Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexesJason S Lamoureux
Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Structure 14:555-65. 2006..The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps... - Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJJun Lu
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
J Bacteriol 194:3670-7. 2012..We propose that the regulation of TraJ dimerization and/or its susceptibility to HslVU could be a key mechanism in various signaling processes for controlling bacterial conjugation in response to physiological or environmental stimuli... - Mapping interactions between the RNA chaperone FinO and its RNA targetsDavid C Arthur
Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
Nucleic Acids Res 39:4450-63. 2011..Together, these data lead us to propose a model of how FinO mediates FinP/traJ mRNA pairing to down regulate bacterial conjugation... - Myosin-driven peroxisome partitioning in S. cerevisiaeAndrei Fagarasanu
Department of Cell Biology and 2 Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G2H7, Canada
J Cell Biol 186:541-54. 2009.... - Structure of the periplasmic stress response protein CpxPGina L Thede
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
J Bacteriol 193:2149-57. 2011..Three of the six previously characterized cpxP loss-of-function mutations, M59T, Q55P, and Q128H, likely result from a destabilization of the protein fold, whereas the R60Q, D61E, and D61V mutations may alter intermolecular interactions... - Crystal structure of a core spliceosomal protein interfaceMatthew J Schellenberg
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Proc Natl Acad Sci U S A 103:1266-71. 2006..SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome... - BRCT domains: easy as one, two, threeCharles Chung Yun Leung
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
Cell Cycle 10:2461-70. 2011..In this review, we incorporate recent structural and biochemical studies to demonstrate how structural features allow single and tandem BRCT domains to attain a high degree of functional diversity... - Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspectiveJoyce J W Wong
Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada
Mol Microbiol 85:602-17. 2012..The structures of TraI and TraM bound to oriT DNA reveal the basis of specific recognition of DNA for their cognate plasmid. Specificity also exists in TraI and TraM interactions with the transferosome protein TraD... - ProQ is an RNA chaperone that controls ProP levels in Escherichia coliSteven G Chaulk
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochemistry 50:3095-106. 2011..Taken together, these results suggest that ProQ does not regulate proP transcription. It may act as an RNA-binding protein to regulate proP translation... - Impact of BRCA1 BRCT domain missense substitutions on phosphopeptide recognitionNicolas Coquelle
Department of Biochemistry, School of Medicine, University of Alberta, Edmonton, AB, Canada
Biochemistry 50:4579-89. 2011..The R1835P and E1836K variants do not dramatically reduce peptide binding, in spite of the fact that these mutations significantly alter the structure of the walls of the Phe(+3) pocket... - Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon-helix-helix DNA-binding foldJun Lu
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Proc Natl Acad Sci U S A 106:9643-8. 2009..The RHH fold of VirC2 is the first crystal structure representing a group of predicted RHH proteins that facilitate endonucleolytic processing of DNA for horizontal gene transfer... - Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraMJun Lu
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
EMBO J 25:2930-9. 2006..Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation... - Structural consequences of a cancer-causing BRCA1-BRCT missense mutationR Scott Williams
Department of Biochemistry, 437 Medical Sciences Building, University of Alberta, Edmonton T6G 2H7, Canada
J Biol Chem 278:2630-5. 2003..Destabilization and global unfolding of the mutated BRCT domain at physiological temperatures explain the pleiotropic molecular and genetic defects associated with the BRCA1-M1775R protein... - Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesisSteven G Chaulk
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
RNA Biol 8:1105-14. 2011..In summary, we describe the first example of pri-miRNA structure modulating differential expression of constituent miRNAs... - Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylationStephen J Campbell
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
J Biol Chem 287:23900-10. 2012..These findings support the hypothesis that RNF8 is responsible for the initiation of Lys-63-linked ubiquitylation in the DNA damage response, which is subsequently amplified by RNF168... - Tidying up loose ends: the role of polynucleotide kinase/phosphatase in DNA strand break repairMichael Weinfeld
Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, Canada
Trends Biochem Sci 36:262-71. 2011.... - Structure of the sporulation-specific transcription factor Ndt80 bound to DNAJason S Lamoureux
Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada
EMBO J 21:5721-32. 2002..Functional similarities between Ndt80 and the Caenorhabditis elegans p53 homolog suggest an evolutionary link between Ndt80 and the p53 family... - FinO is an RNA chaperone that facilitates sense-antisense RNA interactionsDavid C Arthur
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
EMBO J 22:6346-55. 2003.... - Dimerization of CtIP, a BRCA1- and CtBP-interacting protein, is mediated by an N-terminal coiled-coil motifManu J Dubin
School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
J Biol Chem 279:26932-8. 2004..These results suggest a specific model for CtIP homodimerization via its N terminus and contribute to an improved understanding of how this protein might assemble other factors required for its role as a transcriptional corepressor... - BRCA1 DNA-binding activity is stimulated by BARD1Amanda M Simons
Graduate Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
Cancer Res 66:2012-8. 2006....