J N Mark Glover

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. pmc Impact of BRCA1 BRCT domain missense substitutions on phosphopeptide recognition
    Nicolas Coquelle
    Department of Biochemistry, School of Medicine, University of Alberta, Edmonton, AB, Canada
    Biochemistry 50:4579-89. 2011
  2. pmc The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50
    Ross A Edwards
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochemistry 47:11446-56. 2008
  3. pmc Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM
    Joyce J W Wong
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Nucleic Acids Res 39:6775-88. 2011
  4. pmc Mapping interactions between the RNA chaperone FinO and its RNA targets
    David C Arthur
    Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Nucleic Acids Res 39:4450-63. 2011
  5. pmc Mechanism of DNA substrate recognition by the mammalian DNA repair enzyme, Polynucleotide Kinase
    N K Bernstein
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Nucleic Acids Res 37:6161-73. 2009
  6. ncbi request reprint Interactions between BRCT repeats and phosphoproteins: tangled up in two
    J N Mark Glover
    Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
    Trends Biochem Sci 29:579-85. 2004
  7. ncbi request reprint Insights into the molecular basis of human hereditary breast cancer from studies of the BRCA1 BRCT domain
    J N Mark Glover
    Department of Biochemistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada
    Fam Cancer 5:89-93. 2006
  8. ncbi request reprint Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail
    Megan S Lee
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 280:32053-6. 2005
  9. pmc Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays
    Megan S Lee
    Department of Biochemistry, School of Systems Molecular Medicine, University of Alberta, Edmonton, Alberta, Canada
    Cancer Res 70:4880-90. 2010
  10. pmc FinO is an RNA chaperone that facilitates sense-antisense RNA interactions
    David C Arthur
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    EMBO J 22:6346-55. 2003

Collaborators

Detail Information

Publications43

  1. pmc Impact of BRCA1 BRCT domain missense substitutions on phosphopeptide recognition
    Nicolas Coquelle
    Department of Biochemistry, School of Medicine, University of Alberta, Edmonton, AB, Canada
    Biochemistry 50:4579-89. 2011
    ..The R1835P and E1836K variants do not dramatically reduce peptide binding, in spite of the fact that these mutations significantly alter the structure of the walls of the Phe(+3) pocket...
  2. pmc The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50
    Ross A Edwards
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochemistry 47:11446-56. 2008
    ..BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage signals directly to RNA polymerase...
  3. pmc Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM
    Joyce J W Wong
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Nucleic Acids Res 39:6775-88. 2011
    ....
  4. pmc Mapping interactions between the RNA chaperone FinO and its RNA targets
    David C Arthur
    Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Nucleic Acids Res 39:4450-63. 2011
    ..Together, these data lead us to propose a model of how FinO mediates FinP/traJ mRNA pairing to down regulate bacterial conjugation...
  5. pmc Mechanism of DNA substrate recognition by the mammalian DNA repair enzyme, Polynucleotide Kinase
    N K Bernstein
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Nucleic Acids Res 37:6161-73. 2009
    ..The bipartite DNA-binding surface of the mPNK kinase domain explains its preference for recessed 5'-termini, structures that would be encountered in the course of DNA strand break repair...
  6. ncbi request reprint Interactions between BRCT repeats and phosphoproteins: tangled up in two
    J N Mark Glover
    Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
    Trends Biochem Sci 29:579-85. 2004
    ....
  7. ncbi request reprint Insights into the molecular basis of human hereditary breast cancer from studies of the BRCA1 BRCT domain
    J N Mark Glover
    Department of Biochemistry, University of Alberta, T6G 2H7, Edmonton, AB, Canada
    Fam Cancer 5:89-93. 2006
    ..Missense variants identified in breast cancer screening programs often disrupt these interactions and these molecular defects may lead to an increased cancer risk...
  8. ncbi request reprint Structure of the BRCT repeat domain of MDC1 and its specificity for the free COOH-terminal end of the gamma-H2AX histone tail
    Megan S Lee
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 280:32053-6. 2005
    ....
  9. pmc Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays
    Megan S Lee
    Department of Biochemistry, School of Systems Molecular Medicine, University of Alberta, Edmonton, Alberta, Canada
    Cancer Res 70:4880-90. 2010
    ..The excellent agreement between the structure/function effects of these mutations and available clinical data supports the notion that functional and structure information can be useful in the development of models to assess cancer risk...
  10. pmc FinO is an RNA chaperone that facilitates sense-antisense RNA interactions
    David C Arthur
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    EMBO J 22:6346-55. 2003
    ....
  11. ncbi request reprint Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1
    R Scott Williams
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Nat Struct Mol Biol 11:519-25. 2004
    ..The diminished peptide-binding capacity observed for cancer-associated BRCA1-BRCT variants may explain the enhanced cancer risks associated with these mutations...
  12. pmc Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control
    Charles Chung Yun Leung
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Structure 21:1450-9. 2013
    ..Mutations in this surface reduce binding affinity and recruitment of TopBP1 to γH2AX foci in cells. These studies reveal a different mode of phosphopeptide binding by BRCT domains in the DNA damage response. ..
  13. ncbi request reprint The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase
    Nina K Bernstein
    Department of Biochemistry, 4 74 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Mol Cell 17:657-70. 2005
    ..The FHA domain is linked to the kinase/phosphatase catalytic domain by a flexible tether, and it exhibits a mode of target selection based on electrostatic complementarity between the binding surface and the phosphothreonine peptide...
  14. pmc Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates
    Nicolas Coquelle
    Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
    Proc Natl Acad Sci U S A 108:21022-7. 2011
    ..A positively charged surface distinct from the active site specifically facilitates interactions with double-stranded substrates, providing a complex DNA binding surface that enables the recognition of diverse substrates...
  15. doi request reprint Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1
    Stephen J Campbell
    Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Structure 18:167-76. 2010
    ..A mutation in MDC1 that induces a more BRCA1-like conformation relaxes the binding specificity, allowing the mutant to bind phosphopeptides lacking a -COO(-) terminus...
  16. ncbi request reprint Recognition of 5'-YpG-3' sequences by coupled stacking/hydrogen bonding interactions with amino acid residues
    Jason S Lamoureux
    Department of Biochemistry, University of Alberta, Edmonton, Alta, Canada T6G 2H7
    J Mol Biol 335:399-408. 2004
    ..In most cases, this mode of DNA recognition helps explain the sequence specificity of the protein for its target DNA...
  17. doi request reprint Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation
    Jun Lu
    Departments of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Mol Microbiol 70:89-99. 2008
    ....
  18. pmc Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM
    Jun Lu
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    EMBO J 25:2930-9. 2006
    ..Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation...
  19. pmc Insights from the crystal structure of the sixth BRCT domain of topoisomerase IIbeta binding protein 1
    Charles Chung Yun Leung
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Protein Sci 19:162-7. 2010
    ..We further provide insight into poly(ADP-ribose) binding and sites of potential modification by PARP-1...
  20. ncbi request reprint Structural basis for phosphorylation-dependent signaling in the DNA-damage response
    R Scott Williams
    Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
    Biochem Cell Biol 83:721-7. 2005
    ..Our studies have revealed a flexible mode of recognition that allows PNK to interact with numerous negatively charged substrates...
  21. ncbi request reprint Structural consequences of a cancer-causing BRCA1-BRCT missense mutation
    R Scott Williams
    Department of Biochemistry, 437 Medical Sciences Building, University of Alberta, Edmonton T6G 2H7, Canada
    J Biol Chem 278:2630-5. 2003
    ..Destabilization and global unfolding of the mutated BRCT domain at physiological temperatures explain the pleiotropic molecular and genetic defects associated with the BRCA1-M1775R protein...
  22. pmc Crystal structure of a core spliceosomal protein interface
    Matthew J Schellenberg
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Proc Natl Acad Sci U S A 103:1266-71. 2006
    ..SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome...
  23. ncbi request reprint Characterizing the structural features of RNA/RNA interactions of the F-plasmid FinOP fertility inhibition system
    Michael J Gubbins
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    J Biol Chem 278:27663-71. 2003
    ....
  24. pmc N. meningitidis 1681 is a member of the FinO family of RNA chaperones
    Steven Chaulk
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    RNA Biol 7:812-9. 2010
    ..This study strongly suggests that NMB1681 is a FinO-like RNA chaperone that likely regulates gene expression through RNA-based mechanisms in N. meningitidis...
  25. pmc Myosin-driven peroxisome partitioning in S. cerevisiae
    Andrei Fagarasanu
    Department of Cell Biology and 2 Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G2H7, Canada
    J Cell Biol 186:541-54. 2009
    ....
  26. pmc Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint control
    Charles Chung Yun Leung
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 286:4292-301. 2011
    ..Together with systematic mutagenesis studies, we highlight the role of key contacts in governing the unique specificity of the TopBP1-BACH1 interaction...
  27. ncbi request reprint Detection of protein folding defects caused by BRCA1-BRCT truncation and missense mutations
    R Scott Williams
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 278:53007-16. 2003
    ....
  28. ncbi request reprint Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes
    Jason S Lamoureux
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Structure 14:555-65. 2006
    ..The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps...
  29. pmc Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ
    Jun Lu
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    J Bacteriol 194:3670-7. 2012
    ..We propose that the regulation of TraJ dimerization and/or its susceptibility to HslVU could be a key mechanism in various signaling processes for controlling bacterial conjugation in response to physiological or environmental stimuli...
  30. pmc Structure of the periplasmic stress response protein CpxP
    Gina L Thede
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Bacteriol 193:2149-57. 2011
    ..Three of the six previously characterized cpxP loss-of-function mutations, M59T, Q55P, and Q128H, likely result from a destabilization of the protein fold, whereas the R60Q, D61E, and D61V mutations may alter intermolecular interactions...
  31. pmc BRCT domains: easy as one, two, three
    Charles Chung Yun Leung
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Cell Cycle 10:2461-70. 2011
    ..In this review, we incorporate recent structural and biochemical studies to demonstrate how structural features allow single and tandem BRCT domains to attain a high degree of functional diversity...
  32. doi request reprint Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective
    Joyce J W Wong
    Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada
    Mol Microbiol 85:602-17. 2012
    ..The structures of TraI and TraM bound to oriT DNA reveal the basis of specific recognition of DNA for their cognate plasmid. Specificity also exists in TraI and TraM interactions with the transferosome protein TraD...
  33. doi request reprint ProQ is an RNA chaperone that controls ProP levels in Escherichia coli
    Steven G Chaulk
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochemistry 50:3095-106. 2011
    ..Taken together, these results suggest that ProQ does not regulate proP transcription. It may act as an RNA-binding protein to regulate proP translation...
  34. pmc Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon-helix-helix DNA-binding fold
    Jun Lu
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Proc Natl Acad Sci U S A 106:9643-8. 2009
    ..The RHH fold of VirC2 is the first crystal structure representing a group of predicted RHH proteins that facilitate endonucleolytic processing of DNA for horizontal gene transfer...
  35. pmc Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation
    Stephen J Campbell
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    J Biol Chem 287:23900-10. 2012
    ..These findings support the hypothesis that RNF8 is responsible for the initiation of Lys-63-linked ubiquitylation in the DNA damage response, which is subsequently amplified by RNF168...
  36. doi request reprint Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis
    Steven G Chaulk
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    RNA Biol 8:1105-14. 2011
    ..In summary, we describe the first example of pri-miRNA structure modulating differential expression of constituent miRNAs...
  37. pmc Tidying up loose ends: the role of polynucleotide kinase/phosphatase in DNA strand break repair
    Michael Weinfeld
    Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, Canada
    Trends Biochem Sci 36:262-71. 2011
    ....
  38. pmc Structure of the sporulation-specific transcription factor Ndt80 bound to DNA
    Jason S Lamoureux
    Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada
    EMBO J 21:5721-32. 2002
    ..Functional similarities between Ndt80 and the Caenorhabditis elegans p53 homolog suggest an evolutionary link between Ndt80 and the p53 family...
  39. pmc Probing FinO-FinP RNA interactions by site-directed protein-RNA crosslinking and gelFRET
    Alexandru F Ghetu
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    RNA 8:816-23. 2002
    ..These data suggest that significant conformational adjustments in the protein and/or the RNA accompany complex formation...
  40. pmc Structural mechanisms underlying signaling in the cellular response to DNA double strand breaks
    Inbal Mermershtain
    Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Mutat Res 750:15-22. 2013
    ..Here we review the emerging structural principles that underlie how post-translational protein modifications control protein signaling that emanates from these DNA lesions. ..
  41. pmc FHA Domain pThr Binding Specificity: It's All about Me
    Nicolas Coquelle
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Structure 18:1549-50. 2010
    ..Biochemical, structural, and dynamic simulations analysis allowed Pennell and colleagues to unravel the molecular basis of FHA domain phospho-threonine specificity...
  42. ncbi request reprint BRCA1 DNA-binding activity is stimulated by BARD1
    Amanda M Simons
    Graduate Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
    Cancer Res 66:2012-8. 2006
    ....
  43. ncbi request reprint Dimerization of CtIP, a BRCA1- and CtBP-interacting protein, is mediated by an N-terminal coiled-coil motif
    Manu J Dubin
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 279:26932-8. 2004
    ..These results suggest a specific model for CtIP homodimerization via its N terminus and contribute to an improved understanding of how this protein might assemble other factors required for its role as a transcriptional corepressor...