Graham N George

Summary

Affiliation: University of Saskatchewan
Country: Canada

Publications

  1. ncbi request reprint Mercury binding to the chelation therapy agents DMSA and DMPS and the rational design of custom chelators for mercury
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 17:999-1006. 2004
  2. ncbi request reprint Structure of the active site of sulfite dehydrogenase from Starkeya novella
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 45:7488-92. 2006
  3. pmc X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Synchrotron Radiat 19:875-86. 2012
  4. ncbi request reprint Coordination chemistry at the molybdenum site of sulfite oxidase: redox-induced structural changes in the cysteine 207 to serine mutant
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 43:8456-60. 2004
  5. pmc Interaction of product analogues with the active site of rhodobacter sphaeroides dimethyl sulfoxide reductase
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 46:3097-104. 2007
  6. ncbi request reprint X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 46:2-4. 2007
  7. pmc X-ray absorption spectroscopy as a probe of microbial sulfur biochemistry: the nature of bacterial sulfur globules revisited
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    J Bacteriol 190:6376-83. 2008
  8. doi request reprint Chemical forms of mercury and selenium in fish following digestion with simulated gastric fluid
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 21:2106-10. 2008
  9. pmc The chemical forms of mercury in aged and fresh dental amalgam surfaces
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 22:1761-4. 2009
  10. doi request reprint The chemical forms of mercury in human hair: a study using X-ray absorption spectroscopy
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada
    J Biol Inorg Chem 15:709-15. 2010

Collaborators

Detail Information

Publications78

  1. ncbi request reprint Mercury binding to the chelation therapy agents DMSA and DMPS and the rational design of custom chelators for mercury
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 17:999-1006. 2004
    ..We discuss the design criteria for a mercuric specific chelator molecule or "custom chelator", which might form the basis for an improved clinical treatment...
  2. ncbi request reprint Structure of the active site of sulfite dehydrogenase from Starkeya novella
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 45:7488-92. 2006
    ..73 A and three thiolate Mo-S ligands at 2.42 A, whereas the reduced enzyme has one oxo at 1.74 A, one long oxygen at 2.19 A (characteristic of Mo-OH2), and three Mo-S ligands at 2.40 A...
  3. pmc X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Synchrotron Radiat 19:875-86. 2012
    ..This paper reviews the origins of photo-reduction and photo-oxidation, the impact that they can have on active site structure, and the methods that can be used to provide relief from X-ray-induced photo-chemical artifacts...
  4. ncbi request reprint Coordination chemistry at the molybdenum site of sulfite oxidase: redox-induced structural changes in the cysteine 207 to serine mutant
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 43:8456-60. 2004
    ..The Mo(IV) species can be reoxidized to a mono-oxo Mo(V) species still coordinated to Ser207, which in turn can be further reoxidized to yield the initial tri-oxo Mo(VI) structure with loss of Ser207 ligation...
  5. pmc Interaction of product analogues with the active site of rhodobacter sphaeroides dimethyl sulfoxide reductase
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 46:3097-104. 2007
    ....
  6. ncbi request reprint X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 46:2-4. 2007
    ..Furthermore, our results suggest that the form found in vivo is the monooxobis(molybdopterin) species...
  7. pmc X-ray absorption spectroscopy as a probe of microbial sulfur biochemistry: the nature of bacterial sulfur globules revisited
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    J Bacteriol 190:6376-83. 2008
    ..We examine isolated sulfur globules of Allochromatium vinosum and intact cells of a strain of magnetotactic coccus and show that XAS indicates the presence of a chemical form of sulfur resembling S(8)...
  8. doi request reprint Chemical forms of mercury and selenium in fish following digestion with simulated gastric fluid
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 21:2106-10. 2008
    ..We find that local chemical environments of mercury and selenium do not change upon digestion of the fish with simulated gastric fluid. We discuss the toxicological implications for humans consuming fish...
  9. pmc The chemical forms of mercury in aged and fresh dental amalgam surfaces
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Chem Res Toxicol 22:1761-4. 2009
    ..Whereas the fresh amalgam gives spectra that are typical of metallic mercury, the aged amalgam is predominantly beta-mercuric sulfide. The toxicological implications of these results are discussed...
  10. doi request reprint The chemical forms of mercury in human hair: a study using X-ray absorption spectroscopy
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada
    J Biol Inorg Chem 15:709-15. 2010
    ..No appreciable role was found for selenium in coordinating mercury in hair...
  11. doi request reprint The chemical forms of mercury and selenium in whale skeletal muscle
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, SK S7N 5E2, Canada
    Metallomics 3:1232-7. 2011
    ....
  12. pmc The chemical nature of mercury in human brain following poisoning or environmental exposure
    Malgorzata Korbas
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    ACS Chem Neurosci 1:810-8. 2010
    ..Mercury exposure did not perturb organic selenium levels. These results elucidate a key detoxification pathway in the central nervous system and provide new insights into the appropriate methods for biological monitoring...
  13. doi request reprint X-ray absorption spectroscopy of cuprous-thiolate clusters in Saccharomyces cerevisiae metallothionein
    Limei Zhang
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon S7N5E2, Canada
    Chem Biodivers 5:2042-9. 2008
    ....
  14. pmc MoV electron paramagnetic resonance of sulfite oxidase revisited: the low-pH chloride signal
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 47:2033-8. 2008
    ..Here, we present a re-examination of the CW EPR spectroscopy of the sulfite oxidase low-pH chloride species and provide evidence for direct coordination of molybdenum by chloride...
  15. ncbi request reprint Modified active site coordination in a clinical mutant of sulfite oxidase
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Am Chem Soc 129:9421-8. 2007
    ..This difference in the structure of the molybdenum site explains many of the properties of the mutant enzyme which have previously been reported...
  16. doi request reprint Chemical form matters: differential accumulation of mercury following inorganic and organic mercury exposures in zebrafish larvae
    Malgorzata Korbas
    Molecular and Environmental Science Research Group, Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada
    ACS Chem Biol 7:411-20. 2012
    ..Our results clearly demonstrate that the common differentiation between organic and inorganic mercury is not sufficient to determine the toxicity of various mercury species...
  17. doi request reprint Towards a custom chelator for mercury: evaluation of coordination environments by molecular modeling
    Juxia Fu
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, SK, Canada
    J Biol Inorg Chem 16:15-24. 2011
    ..Finally, we propose a new chelator "tripod" molecule, benzene-1,3,5-triamidopropanethiolate, or "Trithiopod," which is expected to bind Hg(2+) in three-coordinate geometry with very high affinity...
  18. doi request reprint Dynamic accumulation and redistribution of methylmercury in the lens of developing zebrafish embryos and larvae
    Malgorzata Korbas
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, SK, S7N 5E2, Canada
    J Biol Inorg Chem 15:1137-45. 2010
    ..The results indicate that mercury is redistributed from the original target tissue to the eye lens, identifying the developing lens as a major sink for methylmercury in early embryonic and larval stages...
  19. doi request reprint X-ray absorption spectroscopy of a quantitatively Mo(V) dimethyl sulfoxide reductase species
    M Jake Pushie
    Molecular and Environmental Sciences Research Group, Department of Geological Sciences, University of Saskatchewan, SK, S7N 5E2, Canada
    Inorg Chem 52:2830-7. 2013
    ..XAS of an extensively studied Mo(V) form of Rhodobacter sphaeroides DMSO reductase (the high-g split species) shows that previously suggested structures for the active site are likely incorrect...
  20. doi request reprint Molybdenum site structure of Escherichia coli YedY, a novel bacterial oxidoreductase
    M Jake Pushie
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Inorg Chem 50:732-40. 2011
    ..05 Å. Our results indicate that the YedY active site shows considerable similarity to but also important differences from that of reduced forms of sulfite oxidase...
  21. doi request reprint Arsenic K-edge X-ray absorption spectroscopy of arsenic in seafood
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada
    Mol Nutr Food Res 53:552-7. 2009
    ..We find that three different chemical types of arsenic are present, consistent with arsenate, an arsenosugar or tetra-alkyl-arsonium species, and a trivalent arsenic species...
  22. doi request reprint The fictile coordination chemistry of cuprous-thiolate sites in copper chaperones
    M Jake Pushie
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, SK, Canada S7N5E2
    Biochim Biophys Acta 1817:938-47. 2012
    ..This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes...
  23. doi request reprint Nature of halide binding to the molybdenum site of sulfite oxidase
    M Jake Pushie
    Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 50:9406-13. 2011
    ..We show that there is no evidence of direct Mo-I coordination, and that the data are consistent with a structure in which the halide is bound at ∼5 Å from Mo...
  24. ncbi request reprint Molecular mimicry in mercury toxicology
    Ruth E Hoffmeyer
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Canada
    Chem Res Toxicol 19:753-9. 2006
    ....
  25. doi request reprint Localizing the chemical forms of sulfur in vivo using X-ray fluorescence spectroscopic imaging: application to onion (Allium cepa) tissues
    Ingrid J Pickering
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Biochemistry 48:6846-53. 2009
    ..In intact cells, X-ray fluorescence spectroscopic imaging showed elevated levels of sulfoxides in the cytosol and elevated levels of reduced sulfur in the central transport vessels and bundle sheath cells...
  26. ncbi request reprint The chemical form of mitochondrial iron in Friedreich's ataxia
    Bogdan F Gh Popescu
    Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada S7N5E5
    J Inorg Biochem 101:957-66. 2007
    ..We conclude that most of the iron in fibroblast HMF from both affected and unaffected cells is ferrihydrite but only FRDA affected cells mineralize significant iron in mitochondrial ferritin...
  27. pmc Localizing organomercury uptake and accumulation in zebrafish larvae at the tissue and cellular level
    Malgorzata Korbas
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
    Proc Natl Acad Sci U S A 105:12108-12. 2008
    ..This novel approach is a powerful tool for directly investigating the molecular toxicology of heavy metals, and should be equally applicable to the study of a wide range of elements in developing embryos...
  28. doi request reprint Structure of the molybdenum site of Escherichia coli trimethylamine N-oxide reductase
    Limei Zhang
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 47:1074-8. 2008
    ..The presence of multiple active site structures provides a potential explanation for the anomalous features reported from the crystal structure...
  29. ncbi request reprint High-resolution X-ray emission spectroscopy of molybdenum compounds
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 44:2579-81. 2005
    ....
  30. ncbi request reprint Chemical form of selenium in naturally selenium-rich lentils (Lens culinaris L.) from Saskatchewan
    Dil Thavarajah
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan, Canada S7N 5E2
    J Agric Food Chem 55:7337-41. 2007
    ..As the toxicities of certain forms of arsenic and selenium are antagonistic, selenium-rich lentils may have a pivotal role to play in alleviating the chronic arsenic poisoning in Bangladesh...
  31. pmc X-ray absorption spectroscopy at the sulfur K-edge: a new tool to investigate the biochemical mechanisms of neurodegeneration
    Mark J Hackett
    Molecular and Environmental Sciences Group, Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatchewan S7NSE2, Canada
    ACS Chem Neurosci 3:178-85. 2012
    ..X-ray absorption spectroscopy at the sulfur K-edge is now poised for an exciting new range of applications to study thiol redox, methionine oxidation, and the role of taurine and sulfatides during neurodegeneration...
  32. doi request reprint Tracing copper-thiomolybdate complexes in a prospective treatment for Wilson's disease
    Limei Zhang
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Biochemistry 48:891-7. 2009
    ..Our work allows new insights into metabolism of the metal complex in the diseased state...
  33. doi request reprint Molybdenum speciation in uranium mine tailings using X-ray absorption spectroscopy
    Joseph Essilfie-Dughan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada
    Environ Sci Technol 45:455-60. 2011
    ..This suggests that the speciation of Mo in the tailings is dependent in part on the chemistry of the original ore...
  34. ncbi request reprint Localizing the biochemical transformations of arsenate in a hyperaccumulating fern
    Ingrid J Pickering
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Canada
    Environ Sci Technol 40:5010-4. 2006
    ..This study provides important insights into arsenic hyperaccumulation, which may prove useful for phytoremediating arsenic-contaminated sites, and demonstrates the strengths of XAS imaging for distinguishing highly localized species...
  35. doi request reprint Metalloprotein active site structure determination: synergy between X-ray absorption spectroscopy and X-ray crystallography
    Julien J H Cotelesage
    Molecular and Environmental Sciences Research Group, Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E2
    J Inorg Biochem 115:127-37. 2012
    ....
  36. doi request reprint X-ray absorption spectroscopy at a protein crystallography facility: the Canadian Light Source beamline 08B1-1
    Julien J H Cotelesage
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, SK, Canada S7N 5E2
    J Synchrotron Radiat 19:887-91. 2012
    ..Mainstream XAS data-processing software packages are available for the users; assistance with data processing and interpretation by staff is also available upon request...
  37. ncbi request reprint Nature of the catalytically labile oxygen at the active site of xanthine oxidase
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    J Am Chem Soc 127:4518-22. 2005
    ..These results support a mechanism initiated by base-assisted nucleophilic attack of the substrate by Mo-OH...
  38. doi request reprint Prion protein expression level alters regional copper, iron and zinc content in the mouse brain
    M Jake Pushie
    Molecular and Environmental Science Research Group, Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan, S7N 5E2, Canada
    Metallomics 3:206-14. 2011
    ....
  39. ncbi request reprint Structure of frataxin iron cores: an X-ray absorption spectroscopic study
    Helen Nichol
    Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada S7N 5E5
    Biochemistry 42:5971-6. 2003
    ..The extended X-ray absorption fine structure (EXAFS) analysis agrees with previous electron microscopy data showing that frataxin cores are composed of very small ferrihydrite crystallites...
  40. doi request reprint Active-site dynamics and large-scale domain motions of sulfite oxidase: a molecular dynamics study
    M Jake Pushie
    Molecular and Environmental Science Research Group, Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan, S7N 5E2, Canada
    J Phys Chem B 114:3266-75. 2010
    ..Our simulations also probe the dynamics of the active site and surrounding residues, adding a further level of structural and thermodynamic detail in understanding sulfite oxidase function...
  41. doi request reprint Development of a combined K-edge subtraction and fluorescence subtraction imaging system for small animals
    Brian Bewer
    Department of Physics, University of Saskatchewan, 116 Science Place, Saskatoon, SK S7N 5E2, Canada
    Rev Sci Instrum 79:085102. 2008
    ..A description of the system is given as well as preliminary results using an iodine test object...
  42. pmc Use of Soller slits to remove reference foil fluorescence from transmission spectra
    Justin J Tse
    Department of Geological Sciences, University of Saskatchewan, 114 Science Place, Saskatoon, Saskatchewan, Canada S7N 5E2
    J Synchrotron Radiat 18:527-9. 2011
    ..A comparison of blank and standard samples, measured with or without Soller slits or under a worst-case scenario, demonstrates the advantages of Soller slits when analyzing weak signal samples via transmission XAS...
  43. pmc Copper sensing function of Drosophila metal-responsive transcription factor-1 is mediated by a tetranuclear Cu(I) cluster
    Xiaohua Chen
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Nucleic Acids Res 36:3128-38. 2008
    ....
  44. ncbi request reprint Structures of the cuprous-thiolate clusters of the Mac1 and Ace1 transcriptional activators
    Kenneth R Brown
    University of Utah Health Sciences Center, 50 N Medical Drive, 4C312, Salt Lake City, Utah 84132, USA
    Biochemistry 41:6469-76. 2002
    ....
  45. ncbi request reprint Biliary excretion of [(GS)(2)AsSe](-) after intravenous injection of rabbits with arsenite and selenate
    Jürgen Gailer
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
    Chem Res Toxicol 15:1466-71. 2002
    ..The relevance of these results for the human toxicology of arsenic and selenium is discussed...
  46. ncbi request reprint Spectroscopic studies of Pyrococcus furiosus superoxide reductase: implications for active-site structures and the catalytic mechanism
    Michael D Clay
    Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Am Chem Soc 124:788-805. 2002
    ..The structural and electronic properties of the SOR active site are discussed in relation to the role and bonding of the axial cysteine residue and the recent proposals for the catalytic mechanism...
  47. ncbi request reprint Yeast Cox11, a protein essential for cytochrome c oxidase assembly, is a Cu(I)-binding protein
    Heather S Carr
    University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    J Biol Chem 277:31237-42. 2002
    ..Cytochrome c oxidase activity is reduced in these mutants. Thus, the residues important for Cu(I) binding correlate with in vivo function, suggesting that Cu(I) binding is important in Cox11 function...
  48. ncbi request reprint C-terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: sub-femtomolar Cu(I) affinity of three proteins involved in copper trafficking
    Zhiguang Xiao
    School of Chemistry, University of Melbourne, Victoria, 3010, Australia
    J Am Chem Soc 126:3081-90. 2004
    ..In addition, both Ctr1c and its copper-responsive transcription factor Mac1 contain similar clusters which may be important in signaling copper status in yeast...
  49. ncbi request reprint Unraveling the substrate-metal binding site of ferrochelatase: an X-ray absorption spectroscopic study
    Gloria C Ferreira
    Department of Biochemistry and Molecular Biology, College of Medicine, Institute for Biomolecular Science and H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
    Biochemistry 41:4809-18. 2002
    ..This is in marked disagreement with the conclusions from X-ray crystallographic studies of the human enzyme, and possible reasons for this are discussed...
  50. ncbi request reprint The chemical form of mercury in fish
    Hugh H Harris
    Stanford Synchrotron Radiation Laboratory, SLAC, MS 69, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
    Science 301:1203. 2003
  51. ncbi request reprint High-resolution EXAFS of the active site of human sulfite oxidase: comparison with density functional theory and X-ray crystallographic results
    Hugh H Harris
    School of Chemistry, University of Sydney, New South Wales 2006, Australia
    Inorg Chem 45:493-5. 2006
    ..The reduced (Mo(IV)) enzyme shows two Mo-S bond lengths at 2.35 A and one at 2.41 A (assigned to cofactor dithiolene and cysteine, respectively, from DFT), together with one Mo=O at 1.72 A and one Mo-OH(2) at 2.30 A...
  52. ncbi request reprint Reversed-phase high-performance liquid chromatographic separation of inorganic mercury and methylmercury driven by their different coordination chemistry towards thiols
    Andrew J Percy
    Department of Chemistry, University of Calgary, Calgary, Alberta, Canada
    J Chromatogr A 1156:331-9. 2007
    ..The developed method is compatible with inductively coupled plasma atomic emission spectrometry and was applied to analyze spiked human urine...
  53. ncbi request reprint characterization of the cytochrome c oxidase assembly factor Cox19 of Saccharomyces cerevisiae
    Kevin Rigby
    Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA
    J Biol Chem 282:10233-42. 2007
    ..Cysteinyl residues important for Cu(I) binding are also shown to be important for the in vivo function of Cox19. Thus, a correlation exists in the ability to bind Cu(I) and in vivo function...
  54. ncbi request reprint Synthesis, characterization, and biomimetic chemistry of cis-oxosulfidomolybdenum(VI) complexes stabilized by an intramolecular Mo(O)=S...S interaction
    Les J Laughlin
    School of Chemistry, University of Melbourne, Victoria 3010, Australia
    Inorg Chem 46:939-48. 2007
    ..The presence of the Mo(O)=S...S interaction does not radically lengthen the Mo=S bond in the complexes or preclude them from reactions typical of unperturbed oxosulfidomolybdenum(VI) complexes...
  55. ncbi request reprint Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans
    Silke Leimkuhler
    Department of Plant Biology, Technical University Braunschweig, 38023 Braunschweig, Germany
    J Biol Chem 278:20802-11. 2003
    ..The active form contains a full complement of redox centers, whereas in the inactive form the FeSI center is likely to be missing...
  56. ncbi request reprint Electronic structure description of the cis-MoOS unit in models for molybdenum hydroxylases
    Christian J Doonan
    The Department of Chemistry and Biological Chemistry, The University of New Mexico, MSC03 20601 University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    J Am Chem Soc 130:55-65. 2008
    ....
  57. ncbi request reprint Strong poison revisited
    Roger C Prince
    ExxonMobil Biomedical Sciences Inc, 1545 Route 22 East, Annandale, NJ 08801, USA
    J Inorg Biochem 101:1891-3. 2007
    ....
  58. ncbi request reprint X-ray absorption spectroscopy of selenate reductase
    Megan J Maher
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    Inorg Chem 43:402-4. 2004
    ..The enzyme was found to contain Se in a reduced form (probably organic) although the sequence does not indicate the presence of selenocysteine. The Se is coordinated to both a metal (probably Fe) and a lighter scatterer such as carbon...
  59. ncbi request reprint Solution structural studies of molybdate-nucleotide polyanions
    Lyndal M R Hill
    School of Chemistry, University of Melbourne, Victoria 3010, Melbourne, Australia
    J Inorg Biochem 88:274-83. 2002
    ....
  60. ncbi request reprint The seleno bis(S-glutathionyl) arsinium ion is assembled in erythrocyte lysate
    Shawn A Manley
    Department of Chemistry, University of Calgary, 2500 University Drive Northwest, Calgary, Alberta, T2N 1N4, Canada
    Chem Res Toxicol 19:601-7. 2006
    ..Combined, these results demonstrate that [(GS)2AsSe]- is rapidly formed in blood and that erythrocytes are an important site for the in vivo formation of this toxicologically important metabolite...
  61. ncbi request reprint Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions
    Paul A Cobine
    National Research Center for Environmental Toxicology, University of Queensland, Coopers Plains, QLD 4108, Australia
    Biochemistry 41:5822-9. 2002
    ..Gain-of-function mutation of a CopZ homologue demonstrated the necessity of four lysine residues on the chaperone for the interaction with CopY. Taken together, these results suggest a mechanism for copper exchange between CopZ and CopY...
  62. ncbi request reprint Using softer X-ray absorption spectroscopy to probe biological systems
    Barak Akabayov
    Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    J Synchrotron Radiat 12:392-401. 2005
    ..In addition, the required experimental set-up and the difficulties associated with conducting softer XAS experiments on biological samples are presented...
  63. ncbi request reprint Redox interplay of oxo-thio-tungsten centers with sulfur-donor co-ligands
    Simon Thomas
    School of Chemistry, University of Melbourne, Victoria 3010, Australia
    Inorg Chem 42:5909-16. 2003
    ..P.; Laughlin, L. J.; Gable, R. W.; Young, C. G. Inorg. Chem. 1996, 35, 3447)...
  64. ncbi request reprint Models for the molybdenum hydroxylases: synthesis, characterization and reactivity of cis-oxosulfido-Mo(VI) complexes
    Christian J Doonan
    School of Chemistry, University of Melbourne, Victoria 3010, Australia
    J Am Chem Soc 128:305-16. 2006
    ..The oxosulfido-Mo(VI) complexes serve as precursors to biologically relevant Mo(V) and heterobimetallic MoO(mu-S)Cu species and undergo a range of biomimetic reactions...
  65. ncbi request reprint Biochemistry: a cadmium enzyme from a marine diatom
    Todd W Lane
    Biosystems Research Department, Sandia National Laboratories, Livermore, California 94551 0969, USA
    Nature 435:42. 2005
    ....
  66. ncbi request reprint Human Sco1 and Sco2 function as copper-binding proteins
    Yih Chern Horng
    Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA
    J Biol Chem 280:34113-22. 2005
    ..Both the mutant yeast and human proteins were nonfunctional, suggesting the importance of this aspartate for normal function. Taken together, these data suggest that both Cu(I) and Cu(II) binding are critical for normal Sco function...
  67. doi request reprint A new type of metal-binding site in cobalt- and zinc-containing adenylate kinases isolated from sulfate-reducers Desulfovibrio gigas and Desulfovibrio desulfuricans ATCC 27774
    Olga Yu Gavel
    REQUIMTE, Departamento de Quimica, Centro de Quimica Fina e Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    J Inorg Biochem 102:1380-95. 2008
    ..gigas is involved in metal coordination and represents a new type of binding motif that differs from other known zinc-binding sites of AK. Cobalt and zinc play a structural role in stabilizing the LID domain...
  68. ncbi request reprint Synthesis, purification, and structural characterization of the dimethyldiselenoarsinate anion
    Jürgen Gailer
    Institute for Ecological Chemistry, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
    Inorg Chem 41:5426-32. 2002
    ..943 A and two As-Se interactions at 2.279 A. On the basis of these results this novel solution species is identified as the dimethyldiselenoarsinate anion...
  69. ncbi request reprint Tetrathiomolybdate causes formation of hepatic copper-molybdenum clusters in an animal model of Wilson's disease
    Graham N George
    Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, 2575 Sand Hill Road, MS 69, Menlo Park, California 94025, USA
    J Am Chem Soc 125:1704-5. 2003
    ..70 A. Each Cu is coordinated to 3-4 sulfurs at 2.28 A with approximately one Mo neighbor at 2.70 A. These results indicate the formation of a biologically novel molybdenum-copper-sulfur cluster...
  70. ncbi request reprint Sulfur X-ray absorption spectroscopy of living mammalian cells: an enabling tool for sulfur metabolomics. In situ observation of uptake of taurine into MDCK cells
    Manuel Gnida
    Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    Biochemistry 46:14735-41. 2007
    ..We also investigated the polarity of uptake of taurine into MDCK cells, and our results confirmed that uptake in situ is predominantly a function of the basolateral cell surface...
  71. ncbi request reprint The active site of arsenite oxidase from Alcaligenes faecalis
    Thomas Conrads
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 124:11276-7. 2002
    ....
  72. ncbi request reprint Mercury speciation in piscivorous fish from mining-impacted reservoirs
    James S Kuwabara
    U S Geological Survey, 345 Middlefield Road, Mail Stop 439, Menlo Park, California 94025, USA
    Environ Sci Technol 41:2745-9. 2007
    ....
  73. pmc Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus
    Ingrid J Pickering
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA
    Plant Physiol 131:1460-7. 2003
    ..These results also indicate that sulfate and selenate reduction are developmentally correlated, and they suggest important differences between S and Se biochemistries...
  74. ncbi request reprint Removal of a cysteine ligand from rubredoxin: assembly of Fe(2)S(2) and Fe(S-Cys)(3)(OH) centres
    Maddalena Cross
    School of Chemistry, University of Melbourne, Parkville, Victoria 3010, Australia
    J Biol Inorg Chem 7:781-90. 2002
    ..In addition to Fe(S-Cys)(3)(OH) centres, the C9G, C42G and C42A preparations contain a second four-coordinate Fe(III)form in which a ligand appears to be supplied by the protein chain...
  75. ncbi request reprint X-ray absorption spectroscopy of bacterial sulfur globules
    Graham N George
    Stanford Synchrotron Radiation Laboratory, SLAC MS 69, 2575 Sand Hill Road, Menlo Park, California 94025, USA
    Microbiology 148:2267-8; discussion 2268-70. 2002
  76. ncbi request reprint The characterization and role of zinc binding in yeast Cox4
    H Jerome Coyne
    University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, Utah 84132, USA
    J Biol Chem 282:8926-34. 2007
    ..The Zn(II) ion is buried within the domain, consistent with its role in structural stability. Potential functions of this matrix-facing subunit are discussed...
  77. ncbi request reprint CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator
    Tong Liu
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
    Nat Chem Biol 3:60-8. 2007
    ..Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed...
  78. ncbi request reprint Selenium biotransformations in an insect ecosystem: effects of insects on phytoremediation
    Danel B Vickerman
    Department of Entomology, University of California at Riverside, Riverside, California 92521, USA
    Environ Sci Technol 38:3581-6. 2004
    ..exigua damage to M. sativa being used to remove selenium from soils. Moreover, the presence of such insects may improve phytoremediation by increasing biotransformation of inorganic selenium and release of volatile selenium species...