M C Ganoza

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. pmc Ribosome-dependent ATPase interacts with conserved membrane protein in Escherichia coli to modulate protein synthesis and oxidative phosphorylation
    Mohan Babu
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 6:e18510. 2011
  2. pmc A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, Ontario M5G 1L6, Canada
    Antimicrob Agents Chemother 45:2813-9. 2001
  3. ncbi request reprint Identification of a ribosomal ATPase in Escherichia coli cells
    M C Kiel
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canad
    Biochimie 81:1097-108. 1999
  4. ncbi request reprint A proposed role for IF-3 and EF-T in maintaining the specificity of prokaryotic initiation complex formation
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario Canada
    Mol Biol Rep 15:33-8. 1991
  5. ncbi request reprint The ribosome as affinity matrix': efficient purification scheme for translation factors
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Biochimie 78:51-61. 1996
  6. pmc Molecular localization of a ribosome-dependent ATPase on Escherichia coli ribosomes
    J Xu
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6
    Nucleic Acids Res 34:1158-65. 2006
  7. ncbi request reprint Regulation of gene expression at the translational level. The rescue factor reverses thermosensitive protein synthesis in N4316, a conditionally-lethal mutant of Escherichia coli defective in translation
    M C Ganoza
    Banting and Best Department of Medical Research, C H Best Institute, Toronto, Ontario, Canada
    Eur J Biochem 178:663-74. 1989
  8. ncbi request reprint Functional interactions of an Escherichia coli ribosomal ATPase
    M C Kiel
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, ON, Canada M5G 1L6
    Eur J Biochem 268:278-86. 2001
  9. ncbi request reprint A new factor from Escherichia coli affects translocation of mRNA
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    J Biol Chem 270:26377-81. 1995
  10. ncbi request reprint Molecular characterization of the prokaryotic efp gene product involved in a peptidyltransferase reaction
    H Aoki
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Biochimie 79:7-11. 1997

Collaborators

Detail Information

Publications22

  1. pmc Ribosome-dependent ATPase interacts with conserved membrane protein in Escherichia coli to modulate protein synthesis and oxidative phosphorylation
    Mohan Babu
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 6:e18510. 2011
    ..Our results suggest that RbbA appears to function together with YhjD as part of a regulatory network that impacts bacterial oxidative phosphorylation and translation efficiency...
  2. pmc A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, Ontario M5G 1L6, Canada
    Antimicrob Agents Chemother 45:2813-9. 2001
    ..The binding site of hygromycin B and its known dual effect on the fidelity of decoding and translocation suggest a model for the action of this drug on ribosomes...
  3. ncbi request reprint Identification of a ribosomal ATPase in Escherichia coli cells
    M C Kiel
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canad
    Biochimie 81:1097-108. 1999
    ..The discovery of a ribosomal ATPase in a prokaryotic cell suggests a common, conserved function for these proteins in translation...
  4. ncbi request reprint A proposed role for IF-3 and EF-T in maintaining the specificity of prokaryotic initiation complex formation
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario Canada
    Mol Biol Rep 15:33-8. 1991
    ..We propose that one function of EF-T may be to prevent the entry of aminoacyl-tRNAs into the 30S particle during initiation. The possibility that a special site for fMet-tRNA resides on 16S rRNA is also discussed...
  5. ncbi request reprint The ribosome as affinity matrix': efficient purification scheme for translation factors
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Biochimie 78:51-61. 1996
    ..The procedure markedly simplifies the isolation, in homogeneous form, of all the non-ribosomal proteins required to reconstruct translation...
  6. pmc Molecular localization of a ribosome-dependent ATPase on Escherichia coli ribosomes
    J Xu
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6
    Nucleic Acids Res 34:1158-65. 2006
    ..The combined data suggest plausible mechanisms for the function of RbbA in translation...
  7. ncbi request reprint Regulation of gene expression at the translational level. The rescue factor reverses thermosensitive protein synthesis in N4316, a conditionally-lethal mutant of Escherichia coli defective in translation
    M C Ganoza
    Banting and Best Department of Medical Research, C H Best Institute, Toronto, Ontario, Canada
    Eur J Biochem 178:663-74. 1989
    ..Based on these and other observations, we propose tht rescue mediates a novel function in the association/dissociation of ribosomal subunits which is essential to the accuracy and efficiency of translation...
  8. ncbi request reprint Functional interactions of an Escherichia coli ribosomal ATPase
    M C Kiel
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, ON, Canada M5G 1L6
    Eur J Biochem 268:278-86. 2001
    ..A possible role for RbbA in protein-chain elongation is proposed...
  9. ncbi request reprint A new factor from Escherichia coli affects translocation of mRNA
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    J Biol Chem 270:26377-81. 1995
    ..We propose that W functions by ejecting tRNAs from ribosomes in a step that precedes the movement of mRNA during translocation...
  10. ncbi request reprint Molecular characterization of the prokaryotic efp gene product involved in a peptidyltransferase reaction
    H Aoki
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Biochimie 79:7-11. 1997
    ..This observation and the requirement for L16 but not for the L7/L12 nor L6 or L11 r-proteins suggest that the binding site for EF-P may overlap the peptidyltransferase center of the ribosome...
  11. ncbi request reprint The gene encoding the elongation factor P protein is essential for viability and is required for protein synthesis
    H Aoki
    Banting and Best Department of Medical Research, Nucleic Acids, Protein Synthesis and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 1L6, Canada
    J Biol Chem 272:32254-9. 1997
    ..At 44 degrees C, mutant cells are severely defective in peptide-bond formation. We conclude that the efp gene is essential for cell viability and is required for protein synthesis...
  12. ncbi request reprint Molecular characterization of a prokaryotic translation factor homologous to the eukaryotic initiation factor eIF4A
    J Lu
    Banting and Best Department of Medical Research, University of Toronto, Ont, Canada
    Int J Biochem Cell Biol 31:215-29. 1999
    ..The W2 protein is not required in standard initiation assays programmed by synthetic mRNAs of defined sequence that lack this feature. We conclude that W2 is an important factor for initiation in eukaryotic and prokaryotic cells...
  13. ncbi request reprint Peptide bond synthesis: function of the efp gene product
    M C Ganoza
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Biol Chem 381:553-9. 2000
    ....
  14. pmc Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis
    H Aoki
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Nucleic Acids Res 19:6215-20. 1991
    ..Based on DNA sequence analysis of this gene, the EF-P protein consists of 187 amino acids with a calculated molecular weight of 20,447. The sequence and chromosomal location of EF-P establishes it as a unique gene product...
  15. ncbi request reprint The asparaginyl-tRNA synthetase gene encodes one of the complementing factors for thermosensitive translation in the Escherichia coli mutant strain, N4316
    H Aoki
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Eur J Biochem 209:511-21. 1992
    ..A potential role of this synthetase in restoring normal protein synthesis with respect to ribosomal frameshifting, read-through of nonsense codons and protein copy number is discussed...
  16. ncbi request reprint Characterization and site of action of a soluble protein that stimulates peptide-bond synthesis
    B R Glick
    Eur J Biochem 71:483-91. 1976
    ..Unlike all other propagation factors, EF-P does not require the 50-S ribosomal proteins L7 and L12 and may therefore occupy a different ribosomal site...
  17. ncbi request reprint Peptide bond formation stimulated by protein synthesis factor EF-P depends on the aminoacyl moiety of the acceptor
    B R Glick
    Eur J Biochem 97:23-8. 1979
    ..One role of EF-P in protein synthesis may be to allow peptide bond synthesis to occur more efficiently with some aminoacyl-tRNAs that are poor acceptors for the ribosomal peptidyl transferase...
  18. pmc Identification of a soluble protein that stimulates peptide bond synthesis
    B R Glick
    Proc Natl Acad Sci U S A 72:4257-60. 1975
    ..We suggest that this extraribosomal factor modulates the intrinsic activity of ribosomes to catalyze peptide-bond synthesis, and regard it as a new factor required for peptide chain elongation, which we call EF-P...
  19. ncbi request reprint Isolation of a soluble factor needed for protein synthesis with various messenger ribonucleic acids other than poly(U)
    M C Ganoza
    J Biol Chem 249:1037-43. 1974
  20. ncbi request reprint Stimulation of peptidyltransferase reactions by a soluble protein
    M C Ganoza
    Eur J Biochem 146:287-94. 1985
    ..30S subunits were resistant to treatment by this reagent. These results suggest the involvement of histidine residues in peptidyltransferase activities. The role of EF-P in the catalytic mechanism of peptidyltransferase is discussed...
  21. ncbi request reprint Identification and quantitation of elongation factor EF-P in Escherichia coli cell-free extracts
    G An
    Can J Biochem 58:1312-4. 1980
    ..It is also shown that a highly purified preparation of EF-P is free of all known protein synthesis factors and ribosomal proteins...
  22. ncbi request reprint Purification and Best Department of Medical Research, University of Toronto, Ont., Canada
    B R Glick
    Can J Biochem 57:749-57. 1979
    ..1 nm), and a frictional coefficient of 1.48, suggesting an asymmetric structure. By this and a number of other criteria, EF-P is a new factor that controls peptide bond formation during protein biosynthesis...