Marie E Fraser

Summary

Affiliation: University of Western Ontario
Country: Canada

Publications

  1. ncbi Two glutamate residues, Glu 208 alpha and Glu 197 beta, are crucial for phosphorylation and dephosphorylation of the active-site histidine residue in succinyl-CoA synthetase
    Marie E Fraser
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochemistry 41:537-46. 2002
  2. ncbi Participation of Cys123alpha of Escherichia coli succinyl-CoA synthetase in catalysis
    Esther Hidber
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    Acta Crystallogr D Biol Crystallogr 63:876-84. 2007
  3. ncbi Structure of the mammalian CoA transferase from pig heart
    Katherine S Bateman
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    Biochemistry 41:14455-62. 2002
  4. ncbi Beta-galactosidase (Escherichia coli) has a second catalytically important Mg2+ site
    Gopinath Sutendra
    Biochemistry, Faculty of Science, University of Calgary, Calgary, Alta, Canada T2N 1N4
    Biochem Biophys Res Commun 352:566-70. 2007
  5. pmc Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary AB T2N 1N4, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:627-30. 2006
  6. ncbi Interactions of GTP with the ATP-grasp domain of GTP-specific succinyl-CoA synthetase
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 281:11058-65. 2006
  7. ncbi Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases
    Erumbi S Rangarajan
    Department of Biochemistry, McGill University, Quebec, Canada
    J Biol Chem 280:42919-28. 2005
  8. ncbi Structure of the CoA transferase from pig heart to 1.7 A resolution
    Abbie M Coros
    Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada
    Acta Crystallogr D Biol Crystallogr 60:1717-25. 2004
  9. ncbi Identification of the cysteine residue exposed by the conformational change in pig heart succinyl-CoA:3-ketoacid coenzyme A transferase on binding coenzyme A
    Stephanie D Tammam
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    Biochemistry 46:10852-63. 2007
  10. ncbi An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation
    Lusong Luo
    Department of Enzymology and Mechanistic Pharmacology, MMPD CEDD, GlaxoSmithKline, Collegeville, PA 19426, USA
    Anal Biochem 353:69-74. 2006

Collaborators

Detail Information

Publications13

  1. ncbi Two glutamate residues, Glu 208 alpha and Glu 197 beta, are crucial for phosphorylation and dephosphorylation of the active-site histidine residue in succinyl-CoA synthetase
    Marie E Fraser
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochemistry 41:537-46. 2002
    ..We postulate that in this conformation, the protein cannot productively bind succinyl-CoA for phosphorylation via succinyl-CoA and P(i)...
  2. ncbi Participation of Cys123alpha of Escherichia coli succinyl-CoA synthetase in catalysis
    Esther Hidber
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    Acta Crystallogr D Biol Crystallogr 63:876-84. 2007
    ..These results explain why this cysteine residue is conserved in the sequences of succinyl-CoA synthetases from different sources...
  3. ncbi Structure of the mammalian CoA transferase from pig heart
    Katherine S Bateman
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    Biochemistry 41:14455-62. 2002
    ....
  4. ncbi Beta-galactosidase (Escherichia coli) has a second catalytically important Mg2+ site
    Gopinath Sutendra
    Biochemistry, Faculty of Science, University of Calgary, Calgary, Alta, Canada T2N 1N4
    Biochem Biophys Res Commun 352:566-70. 2007
    ..These inhibitor and temperature results suggest that binding of the second Mg2+ helps to order the active site for stabilization of the transition state...
  5. pmc Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary AB T2N 1N4, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:627-30. 2006
    ..Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA...
  6. ncbi Interactions of GTP with the ATP-grasp domain of GTP-specific succinyl-CoA synthetase
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 281:11058-65. 2006
    ..The complex of GTP with succinyl-CoA synthetase shows that the enzyme is able to protect GTP from hydrolysis when the active-site histidine residue is not in position to be phosphorylated...
  7. ncbi Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases
    Erumbi S Rangarajan
    Department of Biochemistry, McGill University, Quebec, Canada
    J Biol Chem 280:42919-28. 2005
    ..The structure of the YdiF complex provides a basis for understanding the different catalytic steps in the reaction of Family I CoA transferases...
  8. ncbi Structure of the CoA transferase from pig heart to 1.7 A resolution
    Abbie M Coros
    Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada
    Acta Crystallogr D Biol Crystallogr 60:1717-25. 2004
    ..The structures of a deletion mutant in two different crystal forms were also solved...
  9. ncbi Identification of the cysteine residue exposed by the conformational change in pig heart succinyl-CoA:3-ketoacid coenzyme A transferase on binding coenzyme A
    Stephanie D Tammam
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    Biochemistry 46:10852-63. 2007
    ..Modeling this conformational change has led to the proposal of a new mechanism for catalysis by SCOT...
  10. ncbi An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation
    Lusong Luo
    Department of Enzymology and Mechanistic Pharmacology, MMPD CEDD, GlaxoSmithKline, Collegeville, PA 19426, USA
    Anal Biochem 353:69-74. 2006
    ..This assay is amenable to miniaturization and easily adapted to a format suitable for high-throughput screening; thus, it will be a valuable tool to study Fe(II)/2-oxoglutarate-dependent dioxygenases...
  11. pmc Cyclic nucleotide binding proteins in the Arabidopsis thaliana and Oryza sativa genomes
    Dave Bridges
    Department of Biological Sciences, University of Calgary, AB, T2N 1N4 Canada
    BMC Bioinformatics 6:6. 2005
    ....
  12. pmc Cloning, expression, purification, crystallization and preliminary X-ray analysis of Thermus aquaticus succinyl-CoA synthetase
    Michael A Joyce
    Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:399-402. 2007
    ..The resulting crystals, which diffract to 2.35 A resolution, are of the protein in complex with Mn2+-GDP...
  13. ncbi Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 279:27511-7. 2004
    ..Any of these structural differences might result in different mechanisms of action of the two toxins and the development of hemolytic uremic syndrome upon exposure to Stx2...