Ayman O S El-Kadi

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. pmc Harmine and harmaline downregulate TCDD-induced Cyp1a1 in the livers and lungs of C57BL/6 mice
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E1
    Biomed Res Int 2013:258095. 2013
  2. pmc Camel milk modulates the expression of aryl hydrocarbon receptor-regulated genes, Cyp1a1, Nqo1, and Gsta1, in murine hepatoma Hepa 1c1c7 cells
    Hesham M Korashy
    Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451 Riyadh, Saudi Arabia
    J Biomed Biotechnol 2012:782642. 2012
  3. ncbi request reprint The effect of liver cirrhosis on the regulation and expression of drug metabolizing enzymes
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 5:157-67. 2004
  4. ncbi request reprint Down-regulation of the carcinogen-metabolizing enzyme cytochrome P450 1a1 by vanadium
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada
    Drug Metab Dispos 36:1819-27. 2008
  5. ncbi request reprint Induction of cytochrome P450 1A1 by ketoconazole and itraconazole but not fluconazole in murine and human hepatoma cell lines
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Sci 97:32-43. 2007
  6. doi request reprint Alteration of cardiac cytochrome P450-mediated arachidonic acid metabolism in response to lipopolysaccharide-induced acute systemic inflammation
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Pharmacol Res 61:410-8. 2010
  7. ncbi request reprint Modulation of TCDD-mediated induction of cytochrome P450 1A1 by mercury, lead, and copper in human HepG2 cell line
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Toxicol In Vitro 22:154-8. 2008
  8. ncbi request reprint Transcriptional regulation of the NAD(P)H:quinone oxidoreductase 1 and glutathione S-transferase ya genes by mercury, lead, and copper
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Drug Metab Dispos 34:152-65. 2006
  9. doi request reprint Acute doxorubicin cardiotoxicity alters cardiac cytochrome P450 expression and arachidonic acid metabolism in rats
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Appl Pharmacol 242:38-46. 2010
  10. ncbi request reprint Role of nitric oxide in downregulation of cytochrome P450 1a1 and NADPH: Quinone oxidoreductase 1 by tumor necrosis factor-alpha and lipopolysaccharide
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Pharm Sci 96:2795-807. 2007

Collaborators

Detail Information

Publications84

  1. pmc Harmine and harmaline downregulate TCDD-induced Cyp1a1 in the livers and lungs of C57BL/6 mice
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E1
    Biomed Res Int 2013:258095. 2013
    ..We concluded that harmine and harmaline are promising candidate to inhibit TCDD-mediated induction of Cyp1a1 in mice hepatic and extrahepatic tissues...
  2. pmc Camel milk modulates the expression of aryl hydrocarbon receptor-regulated genes, Cyp1a1, Nqo1, and Gsta1, in murine hepatoma Hepa 1c1c7 cells
    Hesham M Korashy
    Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451 Riyadh, Saudi Arabia
    J Biomed Biotechnol 2012:782642. 2012
    ..In conclusion, camel milk modulates the expression of Cyp1a1, Nqo1, and Gsta1 at the transcriptional and posttranscriptional levels...
  3. ncbi request reprint The effect of liver cirrhosis on the regulation and expression of drug metabolizing enzymes
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 5:157-67. 2004
    ..The reduction in drug metabolizing enzyme activity must be taken into consideration when adjusting doses, especially in patients with severe liver disease...
  4. ncbi request reprint Down-regulation of the carcinogen-metabolizing enzyme cytochrome P450 1a1 by vanadium
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada
    Drug Metab Dispos 36:1819-27. 2008
    ..This study provides the first evidence that V(5+) down-regulates the expression of Cyp1a1 at the transcriptional level through an ATP-dependent mechanism...
  5. ncbi request reprint Induction of cytochrome P450 1A1 by ketoconazole and itraconazole but not fluconazole in murine and human hepatoma cell lines
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Sci 97:32-43. 2007
    ..This study provides the first evidence for the ability of KTZ and ITZ to induce the CYP1A1 gene expression through an AhR-dependent mechanism, and suggests a novel mechanism of the KTZ- and ITZ-mediated toxicities...
  6. doi request reprint Alteration of cardiac cytochrome P450-mediated arachidonic acid metabolism in response to lipopolysaccharide-induced acute systemic inflammation
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Pharmacol Res 61:410-8. 2010
    ..These changes may be involved in the development and/or progression of cardiovascular diseases by inflammation...
  7. ncbi request reprint Modulation of TCDD-mediated induction of cytochrome P450 1A1 by mercury, lead, and copper in human HepG2 cell line
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Toxicol In Vitro 22:154-8. 2008
    ..Based on these results, exposure to metal/PAH mixtures would differentially modulate PAHs-mediated carcinogenicity...
  8. ncbi request reprint Transcriptional regulation of the NAD(P)H:quinone oxidoreductase 1 and glutathione S-transferase ya genes by mercury, lead, and copper
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Drug Metab Dispos 34:152-65. 2006
    ..This is the first demonstration that heavy metals regulate the expression of Nqo1 and Gst ya genes through a transcriptional mechanism...
  9. doi request reprint Acute doxorubicin cardiotoxicity alters cardiac cytochrome P450 expression and arachidonic acid metabolism in rats
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Appl Pharmacol 242:38-46. 2010
    ..These results may represent a novel mechanism by which this drug causes progressive cardiotoxicity...
  10. ncbi request reprint Role of nitric oxide in downregulation of cytochrome P450 1a1 and NADPH: Quinone oxidoreductase 1 by tumor necrosis factor-alpha and lipopolysaccharide
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Pharm Sci 96:2795-807. 2007
    ..These results show that NO, but not peroxynitrite, may be involved in TNF-alpha- and LPS-mediated downregulation of Cyp1a1 without affecting the downregulation of Nqo1...
  11. ncbi request reprint MG-132 inhibits the TCDD-mediated induction of Cyp1a1 at the catalytic activity but not the mRNA or protein levels in Hepa 1c1c7 cells
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Lett 182:121-6. 2008
    ..In conclusion, the present study demonstrates for the first time that MG-132, despite of increasing Cyp1a1 mRNA expression, it decreases its activity probably through decreasing its heme content...
  12. doi request reprint Effect of mercury on aryl hydrocarbon receptor-regulated genes in the extrahepatic tissues of C57BL/6 mice
    Issa E A Amara
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E1
    Food Chem Toxicol 50:2325-34. 2012
    ..The present study demonstrates that Hg(2+) modulates the constitutive and TCDD-induced AhR-regulated genes in a time-, tissue- and, AhR-regulated enzyme genes manner...
  13. doi request reprint The effect of beta-naphthoflavone on the metabolism of amiodarone by hepatic and extra-hepatic microsomes
    Marwa E Elsherbiny
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Lett 195:147-54. 2010
    ..Amiodarone treatment did not affect basal or induced CYP1A activity...
  14. ncbi request reprint The role of redox-sensitive transcription factors NF-kappaB and AP-1 in the modulation of the Cyp1a1 gene by mercury, lead, and copper
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Free Radic Biol Med 44:795-806. 2008
    ..These results clearly demonstrate that activation of the NF-kappaB and AP-1 signaling pathways is directly involved in the modulation of Cyp1a1 by heavy metals...
  15. doi request reprint Chronic doxorubicin cardiotoxicity modulates cardiac cytochrome P450-mediated arachidonic acid metabolism in rats
    Abdulaziz M S Alsaad
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Drug Metab Dispos 40:2126-35. 2012
    ..Therefore, P450 ω-hydroxylases and sEH might be considered as novel targets to prevent and/or treat DOX cardiotoxicity...
  16. doi request reprint Acute arsenic toxicity alters cytochrome P450 and soluble epoxide hydrolase and their associated arachidonic acid metabolism in C57Bl/6 mouse heart
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Xenobiotica 42:1235-47. 2012
    ..Furthermore, inhibiting sEH might represent a novel therapeutic approach to prevent As(III)-induced hypertrophy...
  17. doi request reprint Acute doxorubicin toxicity differentially alters cytochrome P450 expression and arachidonic acid metabolism in rat kidney and liver
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    Drug Metab Dispos 39:1440-50. 2011
    ..These changes can be attributed to DOX-induced inflammation and resulted in altered P450-mediated arachidonic acid metabolism...
  18. ncbi request reprint Arsenite and cadmium, but not chromium, induce NAD(P)H:quinone oxidoreductase 1 through transcriptional mechanisms, in spite of post-transcriptional modifications
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol In Vitro 22:1184-90. 2008
    ..On the other hand, Cr6+ inhibited inducible Nqo1 activity. It is apparent that metals alter Nqo1 expression at the transcriptional level, through a labile protein-mediated pathway...
  19. doi request reprint Modulation of NAD(P)H:quinone oxidoreductase by vanadium in human hepatoma HepG2 cells
    Ghada Abdelhamid
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Toxicol In Vitro 24:1554-61. 2010
    ..The present study demonstrates for the first time that V(5+) down-regulates NQO1 at the transcriptional and post-translational levels in the human hepatoma HepG2 cells via AhR- and Nrf2-dependent mechanisms...
  20. doi request reprint Transcriptional and posttranscriptional regulation of CYP1A1 by vanadium in human hepatoma HepG2 cells
    Ghada Abdelhamid
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Cell Biol Toxicol 26:421-34. 2010
    ..This study demonstrates for the first time that V(5+) downregulates the expression of CYP1A1 at the transcriptional, posttranscriptional and posttranslational mechanisms in the human hepatoma HepG2 cells...
  21. doi request reprint Modulation of cytochrome P450 1 (Cyp1) by vanadium in hepatic tissue and isolated hepatocyte of C57BL/6 mice
    Ghada Abdelhamid
    Faculty of Pharmacy and Pharmaceutical Sciences, 2142J Katz Group Rexall Centre for Pharmacy and Health Research, University of Alberta, Edmonton, AB, T6G 2E1, Canada
    Arch Toxicol 87:1531-43. 2013
    ..The present study demonstrates that there is a differential modulation of Cyp1a1 by V(5+) in C57BL/6 mice livers and isolated hepatocytes and demonstrates Hb as an in vivo specific modulator...
  22. doi request reprint Acute arsenic treatment alters cytochrome P450 expression and arachidonic acid metabolism in lung, liver and kidney of C57Bl/6 mice
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Xenobiotica 43:719-29. 2013
    ..In conclusion, this is the first demonstration that As(III) alters arachidonic acid metabolism in a tissue specific manner...
  23. doi request reprint Differential modulation of cytochrome P450 1a1 by arsenite in vivo and in vitro in C57BL/6 mice
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E1
    Free Radic Biol Med 58:52-63. 2013
    ..Thus, this study implicates Hb as an in vivo-specific modulator...
  24. ncbi request reprint Induction of cytochrome P450 1a1 by the food flavoring agent, maltol
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol In Vitro 21:685-90. 2007
    ..This is the first demonstration that the food flavoring agent, maltol, can directly induce Cyp1a1 gene expression in an AhR-dependent manner and represents a novel mechanism by which maltol promotes carcinogenicity and toxicity...
  25. doi request reprint Inhibition of heme oxygenase-1 partially reverses the arsenite-mediated decrease of CYP1A1, CYP1A2, CYP3A23, and CYP3A2 catalytic activity in isolated rat hepatocytes
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Drug Metab Dispos 40:504-14. 2012
    ..Furthermore, inhibiting the As(III)-mediated induction of HO-1 partially restores the enzymatic activity of these P450s that was initially decreased by As(III), confirming the direct role of HO-1 in the inhibition of P450s...
  26. doi request reprint 2,3,7,8-Tetrachlorodibenzo-p-dioxin and beta-naphthoflavone induce cellular hypertrophy in H9c2 cells by an aryl hydrocarbon receptor-dependant mechanism
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol In Vitro 24:863-71. 2010
    ..In conclusion, AhR ligands caused a hypertrophic effect in H9c2 cells which was associated with induction of several CYP genes which can be prevented by resveratrol...
  27. ncbi request reprint The role of aryl hydrocarbon receptor and the reactive oxygen species in the modulation of glutathione transferase by heavy metals in murine hepatoma cell lines
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alta, Canada T6G 2N8
    Chem Biol Interact 162:237-48. 2006
    ..We concluded that heavy metals differentially modulate the Gst activity through oxidative stress- and AhR-mediated mechanisms...
  28. ncbi request reprint The stereoselective metabolism of halofantrine to desbutylhalofantrine in the rat: evidence of tissue-specific enantioselectivity in microsomal metabolism
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Chirality 19:22-33. 2007
    ..In vivo, oral (+/-)-KTZ caused significant increases in plasma halofantrine and decreases in DHF enantiomer plasma concentrations...
  29. doi request reprint Acute mercury toxicity modulates cytochrome P450, soluble epoxide hydrolase and their associated arachidonic acid metabolites in C57Bl/6 mouse heart
    Issa E A Amara
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, T6G 2E1, AB, Canada
    Toxicol Lett 226:53-62. 2014
    ..Furthermore, inhibiting sEH might represent a novel therapeutic approach to prevent Hg(2+)-induced hypertrophy...
  30. doi request reprint Sulforaphane induces CYP1A1 mRNA, protein, and catalytic activity levels via an AhR-dependent pathway in murine hepatoma Hepa 1c1c7 and human HepG2 cells
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Cancer Lett 275:93-101. 2009
    ..This is the first demonstration that the broccoli-derived SUL can directly induce Cyp1a1 gene expression in an AhR-dependent manner and represents a novel mechanism by which SUL induces this enzyme...
  31. ncbi request reprint Regulatory mechanisms modulating the expression of cytochrome P450 1A1 gene by heavy metals
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Sci 88:39-51. 2005
    ..This is the first demonstration that heavy metals can directly induce Cyp1a1 gene expression in an AhR-dependent manner through transcriptional and posttranslational mechanisms...
  32. doi request reprint Cytochrome P450 epoxygenase metabolite, 14,15-EET, protects against isoproterenol-induced cellular hypertrophy in H9c2 rat cell line
    Mandy M Y Tse
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada
    Vascul Pharmacol 58:363-73. 2013
    ..Furthermore, 14,15-EET exerts a protective effect against isoproterenol-induced cellular hypertrophy whereas, 20-HETE induced cellular hypertrophy in H9c2 cells...
  33. ncbi request reprint Ascorbic acid differentially modulates the induction of heme oxygenase-1, NAD(P)H:quinone oxidoreductase 1 and glutathione S-transferase Ya by As(3+), Cd(2+) and Cr(6+)
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alta, Canada
    Cancer Lett 246:54-62. 2007
    ..Hence, it is tempting to speculate that AA may potentiate the therapeutic efficacy of As(3+) by enhancing the expression of HO-1, Nqo1, and Gst ya while acting as a potent antioxidant...
  34. doi request reprint Differential modulation of aryl hydrocarbon receptor regulated enzymes by arsenite in the kidney, lung, and heart of C57BL/6 mice
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, 2142J Katz Group Rexall Centre for Pharmacy and Health Research, University of Alberta, Edmonton, AB T6G 2N8, Canada
    Arch Toxicol 86:897-910. 2012
    ..In conclusion, the present study demonstrates for the first time that As(III) modulates constitutive and TCDD-induced AhR-regulated genes in a time-, tissue-, and AhR-regulated enzyme-specific manner...
  35. doi request reprint Inhibition of soluble epoxide hydrolase confers cardioprotection and prevents cardiac cytochrome P450 induction by benzo(a)pyrene
    Mona E Aboutabl
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    J Cardiovasc Pharmacol 57:273-81. 2011
    ..The current study demonstrates the cardioprotective effect of sEH inhibitor, TUPS, against BaP-induced cardiac hypertrophy and further confirms the role of sEH and CYP450 enzymes in the development of cardiac hypertrophy...
  36. ncbi request reprint The role of oxidative stress in the modulation of aryl hydrocarbon receptor-regulated genes by As3+, Cd2+, and Cr6+
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3118 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Free Radic Biol Med 39:1499-511. 2005
    ..Our data clearly show that As(3+)-, Cd(2+)-, and Cr(6+)-induced oxidative stress modulates Cyp1a1 at transcriptional and posttranscriptional levels but induces Nqo1 and Gst ya at the transcriptional level...
  37. doi request reprint NF-kappaB and AP-1 are key signaling pathways in the modulation of NAD(P)H:quinone oxidoreductase 1 gene by mercury, lead, and copper
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    J Biochem Mol Toxicol 22:274-83. 2008
    ..These results clearly demonstrate that activation of NF-kappaB negatively regulates the expression of Nqo1 by heavy metals, whereas AP-1 signaling pathways differentially modulates the heavy metal-mediated effects...
  38. pmc Camel milk triggers apoptotic signaling pathways in human hepatoma HepG2 and breast cancer MCF7 cell lines through transcriptional mechanism
    Hesham M Korashy
    Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
    J Biomed Biotechnol 2012:593195. 2012
    ..Taken together, camel milk inhibited HepG2 and MCF7 cells survival and proliferation through the activation of both the extrinsic and intrinsic apoptotic pathways...
  39. ncbi request reprint Transcriptional activation and posttranscriptional modification of Cyp1a1 by arsenite, cadmium, and chromium
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada
    Toxicol Lett 172:106-19. 2007
    ..We clearly demonstrated that As(3+), Cd(2+), and Cr(6+) increase Cyp1a1 mRNA levels at the transcriptional and posttranscriptional levels while decreasing Cyp1a1 activity at the posttranslational level...
  40. ncbi request reprint H9c2 cell line is a valuable in vitro model to study the drug metabolizing enzymes in the heart
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    J Pharmacol Toxicol Methods 56:317-22. 2007
    ..However, a major limitation for isolated cardiomyocytes is that they are rather fragile and difficult to isolate. Therefore, there is an urgent need for an in vitro cell line model...
  41. doi request reprint Mercury modulates the cytochrome P450 1a1, 1a2 and 1b1 in C57BL/6J mice: in vivo and in vitro studies
    Issa E A Amara
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E1
    Toxicol Appl Pharmacol 266:419-29. 2013
    ..Moreover, this study implicates Hb as an in vivo specific modulator of Cyp1 family...
  42. pmc Determination of the dominant arachidonic acid cytochrome p450 monooxygenases in rat heart, lung, kidney, and liver: protein expression and metabolite kinetics
    Ahmed A El-Sherbeni
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada, T6G 2E1
    AAPS J 15:112-22. 2013
    ..These results provide important insights into the activities of P450 epoxygenases and P450 hydroxylases-mediated AA metabolism in different organs and their associated P450 protein levels...
  43. doi request reprint Arsenite down-regulates cytochrome P450 1A1 at the transcriptional and posttranslational levels in human HepG2 cells
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Free Radic Biol Med 48:1399-409. 2010
    ..This study demonstrates that As(III) down-regulates CYP1A1 through transcriptional and posttranslational mechanisms. In addition, HO-1 is involved in the As(III)-mediated down-regulation of CYP1A1 at the catalytic activity level...
  44. pmc 3-methylcholanthrene and benzo(a)pyrene modulate cardiac cytochrome P450 gene expression and arachidonic acid metabolism in male Sprague Dawley rats
    Mona E Aboutabl
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    Br J Pharmacol 158:1808-19. 2009
    ..Here, we have investigated the effect of 3-methylcholanthrene (3-MC) and benzo(a)pyrene (BaP), two AhR ligands, on the development of cardiac hypertrophy...
  45. ncbi request reprint Benzo[a]pyrene, 3-methylcholanthrene and beta-naphthoflavone induce oxidative stress in hepatoma hepa 1c1c7 Cells by an AHR-dependent pathway
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3118, Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta T6G 2N8, Canada
    Free Radic Res 38:1191-200. 2004
    ..The AHR-ligand-mediated decrease in cyp1a1 activity was reversed by the antioxidant N-acetylcysteine. Our results show that the AHR-ligands induce oxidative stress by an AHR-dependent pathway...
  46. doi request reprint Effect of bile and lipids on the stereoselective metabolism of halofantrine by rat everted-intestinal sacs
    Jigar P Patel
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Chirality 22:275-83. 2010
    ..The results were consistent with previous in vivo evaluations showing that the desbutylhalofantrine to halofantrine ratio was decreased by the ingestion of a high fat meal...
  47. doi request reprint Mercury modulates the CYP1A1 at transcriptional and posttranslational levels in human hepatoma HepG2 cells
    Issa E A Amara
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Toxicol Lett 199:225-33. 2010
    ..In addition, HO-1 is involved in the modulation of CYP1A1 at the posttranslational level...
  48. doi request reprint Harman induces CYP1A1 enzyme through an aryl hydrocarbon receptor mechanism
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton AB, Canada T6G 2N8
    Toxicol Appl Pharmacol 249:55-64. 2010
    ..We concluded that harman can directly induce CYP1A1 gene expression in an AhR-dependent manner and may represent a novel mechanism by which harman promotes mutagenicity, co-mutagenicity and carcinogenicity...
  49. doi request reprint STAT3 silencing in dendritic cells by siRNA polyplexes encapsulated in PLGA nanoparticles for the modulation of anticancer immune response
    Aws Alshamsan
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Canada
    Mol Pharm 7:1643-54. 2010
    ..Moreover, it can be combined with other means for cancer immunotherapy like cancer vaccine strategies...
  50. doi request reprint Transcriptional and posttranslational mechanisms modulating the expression of the cytochrome P450 1A1 gene by lead in HepG2 cells: a role of heme oxygenase
    Hesham M Korashy
    Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia
    Toxicology 291:113-21. 2012
    ..In conclusion, this study demonstrated that Pb(2+) down-regulates the expression of CYP1A1 through transcriptional and posttranslational mechanisms and confirms the role of HO-1 in a Pb(2+)-mediated effect...
  51. ncbi request reprint Down-regulation of the detoxifying enzyme NAD(P)H:quinone oxidoreductase 1 by vanadium in Hepa 1c1c7 cells
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Toxicol Appl Pharmacol 236:261-9. 2009
    ..In conclusion, the present study demonstrates that V5+ down-regulates Nqo1 at the transcriptional level, possibly through inhibiting the ATP-dependent activation of Nrf2...
  52. ncbi request reprint Induction of the NAD(P)H:quinone oxidoreductase 1 by ketoconazole and itraconazole: a mechanism of cancer chemoprotection
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada
    Cancer Lett 258:135-43. 2007
    ..This study provides the first evidence for the ability of KTZ and ITZ to induce the Nqo1 gene expression through an AhR-dependent mechanism...
  53. doi request reprint Peganum harmala L. is a candidate herbal plant for preventing dioxin mediated effects
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G 2N8, Canada
    Planta Med 76:671-7. 2010
    ..85 % (w/w), respectively. In addition, both of the active alkaloids showed an inhibitory effect on TCDD-induced Cyp1a1 activity level. We concluded that Peganum harmala L. can interfere with AhR ligands-mediated effects...
  54. doi request reprint Modulation of cytochrome P450 gene expression and arachidonic acid metabolism during isoproterenol-induced cardiac hypertrophy in rats
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Drug Metab Dispos 36:2277-86. 2008
    ..In conclusion, isoproterenol-induced cardiac hypertrophy alters arachidonic acid metabolism and its associated P450 enzymes, suggesting their role in the development and/or progression of cardiac hypertrophy...
  55. doi request reprint Camel urine inhibits the cytochrome P450 1a1 gene expression through an AhR-dependent mechanism in Hepa 1c1c7 cell line
    Abdulqader A Alhaider
    Department of Pharmacology, College of Medicine, King Saud University, Riyadh, Saudi Arabia
    J Ethnopharmacol 133:184-90. 2011
    ..Therefore, we examined the ability of three different camel urines (virgin, lactating, and pregnant source) to modulate a well-known cancer-activating enzyme, the cytochrome P450 1a1 (Cyp1a1) in murine hepatoma Hepa 1c1c7 cell line...
  56. pmc Transcriptional and posttranslational inhibition of dioxin-mediated induction of CYP1A1 by harmine and harmol
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol Lett 208:51-61. 2012
    ..We concluded that harmine and its metabolite, harmol, are new inhibitors of dioxin-mediated effects...
  57. doi request reprint Differential effects of soluble epoxide hydrolase inhibition and CYP2J2 overexpression on postischemic cardiac function in aged mice
    Ketul R Chaudhary
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    Prostaglandins Other Lipid Mediat 104:8-17. 2013
    ..However, the loss of protective effects observed in aged CYP2J2 Tr might be attributed to increased levels of DiHOME, oxidative stress and/or decreased PP2A activity. ..
  58. ncbi request reprint Effect of inflammation on molecular targets and drug transporters
    Sherif Hanafy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Pharm Pharm Sci 15:361-75. 2012
    ..In conclusion, inflammation alters the gene expression of some mediators and drug transporters that can influence the behavior of drugs in the body and contribute to therapeutic failure...
  59. ncbi request reprint Down-regulation of aryl hydrocarbon receptor-regulated genes by tumor necrosis factor-alpha and lipopolysaccharide in murine hepatoma Hepa 1c1c7 cells
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, 3118 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada T6G 2N8
    J Pharm Sci 94:493-506. 2005
    ..Furthermore, reactive oxygen species may be involved in the down-regulation of AHR-regulated genes...
  60. doi request reprint The impact of experimental hyperlipidemia on the distribution and metabolism of amiodarone in rat
    Anooshirvan Shayeganpour
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Int J Pharm 361:78-86. 2008
    ..This experimental hyperlipidemia caused a complex pattern of changes in tissue distribution of AM. In addition, there are decreases in the expression of some important rat CYP isoenzymes...
  61. ncbi request reprint 20-Hydroxyeicosatetraenoic acid is a potential therapeutic target in cardiovascular diseases
    Osama H Elshenawy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Curr Drug Metab 14:706-19. 2013
    ..Based on the recent studies, 20-HETE production and/or action might be a therapeutic target to protect against the initiation and progression of cardiovascular diseases. ..
  62. pmc Harmaline and harmalol inhibit the carcinogen-activating enzyme CYP1A1 via transcriptional and posttranslational mechanisms
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Food Chem Toxicol 50:353-62. 2012
    ..These data suggest that harmaline and harmalol are promising agents for preventing dioxin-mediated effects...
  63. doi request reprint Murine atrial HL-1 cell line is a reliable model to study drug metabolizing enzymes in the heart
    Osama H Elshenawy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E1
    Vascul Pharmacol 58:326-33. 2013
    ..In conclusion, HL-1 cells provide a valuable in vitro model to study the role of Cyps and their associated AA metabolites in addition to phase II enzymes in cardiovascular disease states...
  64. doi request reprint Role of cytochrome P450-mediated arachidonic acid metabolites in the pathogenesis of cardiac hypertrophy
    Abdulaziz M S Alsaad
    Faculty of Pharmacy and Pharmaceutical Sciences, 2142J Katz Group Rexall Center for Pharmacy and Health Research, University of Alberta, Edmonton, Alberta, Canada T6G 2E1
    Drug Metab Rev 45:173-95. 2013
    ..However, further understanding of these metabolites and their effects on intracellular cascades will be required to assess their potential translation to therapeutic approaches for the prevention and/or treatment of CH and heart failure...
  65. ncbi request reprint tert-Butylhydroquinone is a novel aryl hydrocarbon receptor ligand
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, 3118 Dentistry Pharmacy Centre, University of Alberta, Edmonton, AB, Canada T6G 2N8
    Drug Metab Dispos 33:365-72. 2005
    ..This is the first demonstration that the phenolic antioxidant, tBHQ, can directly induce Cyp1a1 gene expression in an AHR-dependent manner and may represent a novel mechanism by which tBHQ promotes carcinogenicity...
  66. ncbi request reprint The metabolism of amiodarone by various CYP isoenzymes of human and rat, and the inhibitory influence of ketoconazole
    Marwa E Elsherbiny
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta
    J Pharm Pharm Sci 11:147-59. 2008
    ..To evaluate the metabolism of amiodarone (AM) to desethylamiodarone (DEA) by selected human and rat cytochrome P450, and the inhibitory effect of ketoconazole (KTZ)...
  67. ncbi request reprint Validation of bone marrow derived dendritic cells as an appropriate model to study tumor-mediated suppression of DC maturation through STAT3 hyperactivation
    Aws Alshamsan
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    J Pharm Pharm Sci 13:21-6. 2010
    ..Therefore, we assessed the behavior of two distinct murine DC models upon exposure to tumor-conditioned medium of B16.F10 melanoma (B16-CM)...
  68. ncbi request reprint Chemoprotective and carcinogenic effects of tert-butylhydroquinone and its metabolites
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 8:1-7. 2007
    ..This review provides an account of recent mechanisms proposed for both chemoprotective and carcinogenic effect of tBHQ...
  69. doi request reprint Induction of several cytochrome P450 genes by doxorubicin in H9c2 cells
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Vascul Pharmacol 49:166-72. 2008
    ..Doxorubicin-mediated CYP induction may represent a novel mechanism by which this drug induces cardiotoxicity...
  70. doi request reprint Effect of cytochrome P450 polymorphism on arachidonic acid metabolism and their impact on cardiovascular diseases
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Pharmacol Ther 125:446-63. 2010
    ..Therefore, more research is needed to confirm this association and to better understand the pathophysiologic mechanisms behind it...
  71. ncbi request reprint Role of NF-kappaB in the regulation of cytochrome P450 enzymes
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 10:164-78. 2009
    ..In conclusion, NF-kappaB plays a crucial role in the regulation of CYP through several mechanisms and this role can explain the altered CYP regulation in many conditions...
  72. ncbi request reprint Modulation of aryl hydrocarbon receptor-regulated gene expression by arsenite, cadmium, and chromium
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3118 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alta, T6G 2N8, Canada
    Toxicology 202:249-69. 2004
    ..These results show that the ability of metals to alter the capacity of AhR ligands to induce the bioactivating phase I and the detoxifying phase II enzymes will influence the carcinogenicity and mutagenicity of the AhR ligands...
  73. ncbi request reprint Constitutive expression and inducibility of CYP1A1 in the H9c2 rat cardiomyoblast cells
    Mona E Aboutabl
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol In Vitro 21:1686-91. 2007
    ..Therefore, this cell line offers a unique in vitro model to study the role of CYP1A1 in the pathogenesis of various cardiovascular diseases...
  74. ncbi request reprint Assessment of the inactivation potential of desethylamiodarone on human CYP1A1
    Marwa E Elsherbiny
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Drug Metab Lett 4:9-14. 2010
    ..1 microM and 9.6 microM, respectively) of CYP1A1 by desethylamiodarone. NADPH addition and/or replenishment were found to be important factors in determining the control activity in inactivation studies...
  75. ncbi request reprint Modulation of cardiac and hepatic cytochrome P450 enzymes during heart failure
    Beshay N M Zordoky
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 9:122-8. 2008
    ..Therefore, more research is needed to elucidate the mechanisms by which CYP affect the development and/or progression of heart failure and also the mechanism by which heart failure alters cardiac and hepatic CYP enzymes...
  76. doi request reprint Transcriptional modulation of the NAD(P)H:quinone oxidoreductase 1 by mercury in human hepatoma HepG2 cells
    Issa E A Amara
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    Free Radic Biol Med 51:1675-85. 2011
    ..In conclusion, we demonstrated that Hg(2+) regulates the expression of the NQO1 gene through a transcriptional mechanism in human hepatoma HepG2 cells. In addition, Nrf2 is involved in the modulation of NQO1 by Hg(2+)...
  77. doi request reprint The effect of Nrf2 knockout on the constitutive expression of drug metabolizing enzymes and transporters in C57Bl/6 mice livers
    Anwar Anwar-Mohamed
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Toxicol In Vitro 25:785-95. 2011
    ..In conclusion, knockout of Nrf2 causes disruption to the coordination of phase I, phase II drug DMEs, and phase III drug transporters through altering the transcription factors controlling them...
  78. ncbi request reprint Peganum harmala L. differentially modulates cytochrome P450 gene expression in human hepatoma HepG2 cells
    Mohamed A M El Gendy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton AB, T6G 2N8, Canada
    Drug Metab Lett 3:212-6. 2009
    ..harmala extract significantly increased the expression of CYP1A2, 2C19, and 3A4 whereas; CYP 2B6, 2D6 and 2E1 was significantly decreased. We concluded that care should be taken when P. harmala is co-administered with other drugs...
  79. ncbi request reprint Differential effects of mercury, lead and copper on the constitutive and inducible expression of aryl hydrocarbon receptor (AHR)-regulated genes in cultured hepatoma Hepa 1c1c7 cells
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alta, T6G 2N8, Canada
    Toxicology 201:153-72. 2004
    ..These results demonstrate that heavy metals differentially modulate the constitutive and the inducible expression of AHR-regulated genes...
  80. ncbi request reprint The role of aryl hydrocarbon receptor in the pathogenesis of cardiovascular diseases
    Hesham M Korashy
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Drug Metab Rev 38:411-50. 2006
    ....
  81. ncbi request reprint Determination of the enzyme(s) involved in the metabolism of amiodarone in liver and intestine of rat: the contribution of cytochrome P450 3A isoforms
    Anooshirvan Shayeganpour
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Canada T6G 2N8
    Drug Metab Dispos 34:43-50. 2006
    ..v. treated rats. Although low to undetectable concentrations of DEA were a limitation for determination of AUC of DEA in vivo, it was confirmed that ketoconazole could cause a significant increase in AM concentrations in rat...
  82. ncbi request reprint Cytochrome P450 enzymes: central players in cardiovascular health and disease
    Reem H Elbekai
    Faculty of Pharmacy and Pharmaceutical Sciences, 3126 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Pharmacol Ther 112:564-87. 2006
    ..However, more research is needed to allow the integration of this information into a clinical setting...
  83. ncbi request reprint Expression of cytochrome P450 in lung tumor
    Negar Gharavi
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2N8
    Curr Drug Metab 5:203-10. 2004
    ..This review gives a detailed picture of the expression of CYP450 in lung tumor and the role of this enzyme in lung tumor in the fate of anticancer drugs...
  84. doi request reprint The induction of tumor apoptosis in B16 melanoma following STAT3 siRNA delivery with a lipid-substituted polyethylenimine
    Aws Alshamsan
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    Biomaterials 31:1420-8. 2010
    ....