Aled Edwards

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. pmc A generalizable pre-clinical research approach for orphan disease therapy
    Chandree L Beaulieu
    Children s Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa, ON, Canada
    Orphanet J Rare Dis 7:39. 2012
  2. pmc MAID : an effect size based model for microarray data integration across laboratories and platforms
    Ivan Borozan
    Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, ON M5G1L6, Canada
    BMC Bioinformatics 9:305. 2008
  3. ncbi request reprint Protein production: feeding the crystallographers and NMR spectroscopists
    A M Edwards
    Ontario Cancer Institute, Toronto, Canada
    Nat Struct Biol 7:970-2. 2000
  4. doi request reprint Large-scale structural biology of the human proteome
    Aled Edwards
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Annu Rev Biochem 78:541-68. 2009
  5. ncbi request reprint Origin DNA-binding proteins
    A M Edwards
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Curr Opin Struct Biol 8:49-53. 1998
  6. doi request reprint Open access chemical and clinical probes to support drug discovery
    Aled M Edwards
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    Nat Chem Biol 5:436-40. 2009
  7. pmc The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta
    Keith I Pardee
    Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada
    PLoS Biol 7:e43. 2009
  8. pmc The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding
    A Bochkarev
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8
    EMBO J 18:4498-504. 1999
  9. pmc In situ proteolysis to generate crystals for structure determination: an update
    Amy Wernimont
    Midwest Center for Structural Genomics and Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 4:e5094. 2009
  10. ncbi request reprint Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA
    A Bochkarev
    Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, Canada
    Nature 385:176-81. 1997

Collaborators

Detail Information

Publications27

  1. pmc A generalizable pre-clinical research approach for orphan disease therapy
    Chandree L Beaulieu
    Children s Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa, ON, Canada
    Orphanet J Rare Dis 7:39. 2012
    ..In addition to enabling academic pre-clinical research, strategies such as this may also aid in seeding startup companies, as well as further engaging the pharmaceutical industry in the treatment of rare genetic disease...
  2. pmc MAID : an effect size based model for microarray data integration across laboratories and platforms
    Ivan Borozan
    Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, ON M5G1L6, Canada
    BMC Bioinformatics 9:305. 2008
    ..The increase in sensitivity and reliability of measurements of gene expression changes can be improved through a systematic integration of different microarray datasets that address the same or similar biological questions...
  3. ncbi request reprint Protein production: feeding the crystallographers and NMR spectroscopists
    A M Edwards
    Ontario Cancer Institute, Toronto, Canada
    Nat Struct Biol 7:970-2. 2000
    ..Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community...
  4. doi request reprint Large-scale structural biology of the human proteome
    Aled Edwards
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Annu Rev Biochem 78:541-68. 2009
    ..Half of the unique human protein structures determined in the past three years derive from these initiatives...
  5. ncbi request reprint Origin DNA-binding proteins
    A M Edwards
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Curr Opin Struct Biol 8:49-53. 1998
    ..One of the origin-binding proteins, Epstein-Barr virus nuclear antigen 1, most likely has two modes of DNA binding; the sequential use of these modes may be important for the initiation of DNA replication...
  6. doi request reprint Open access chemical and clinical probes to support drug discovery
    Aled M Edwards
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    Nat Chem Biol 5:436-40. 2009
    ..Chemical biologists could play a central role in this effort...
  7. pmc The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta
    Keith I Pardee
    Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada
    PLoS Biol 7:e43. 2009
    ..They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions...
  8. pmc The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding
    A Bochkarev
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8
    EMBO J 18:4498-504. 1999
    ..The structure also suggests a mechanism for RPA trimer formation...
  9. pmc In situ proteolysis to generate crystals for structure determination: an update
    Amy Wernimont
    Midwest Center for Structural Genomics and Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 4:e5094. 2009
    ..The overall success rate is similar to that observed in the smaller study, and appears to be higher than any other method reported to rescue stalled protein crystallography projects...
  10. ncbi request reprint Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA
    A Bochkarev
    Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, Canada
    Nature 385:176-81. 1997
    ..The ssDNA lies in a channel that extends from one subdomain to the other. The structure of each RPA70 subdomain is similar to those of the bacteriophage SSBs, indicating that the mechanism of ssDNA-binding is conserved...
  11. ncbi request reprint Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms
    Masoud Vedadi
    Structural Genomics Consortium, U of Toronto, 100 College St Rm 522B, Toronto, Ont, Canada M5G 1L5
    Mol Biochem Parasitol 151:100-10. 2007
    ....
  12. ncbi request reprint The Drosophila nuclear receptor e75 contains heme and is gas responsive
    Jeff Reinking
    Banting and Best Department of Medical Research, Charles H Best Institute, 112 College Street, Toronto, Ontario, M5G 1L6, Canada
    Cell 122:195-207. 2005
    ..Further, the E75-DHR3 interaction is also regulated by the binding of NO or CO to the heme center, suggesting that E75 may also function as a diatomic gas sensor. Possible mechanisms and roles for these interactions are discussed...
  13. pmc Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum
    Bin Wu
    Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    Protein Sci 12:2831-7. 2003
    ..A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA...
  14. ncbi request reprint Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes
    V Saridakis
    Division of Molecular and Structural Biology, Ontario Cancer Institute, 610 University Avenue Toronto M5G 2M9, Ontario, Canada
    J Biol Chem 276:7225-32. 2001
    ..In addition, the role of the conserved (16)HXGH(19) active site motif in catalysis was probed by mutagenic, enzymatic and crystallographic techniques, including the characterization of an NMN(+)/SO4(2-) complex of mutant H19A NMNATase...
  15. ncbi request reprint Structural proteomics of an archaeon
    D Christendat
    Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9
    Nat Struct Biol 7:903-9. 2000
    ..This demonstrates that structural proteomics is feasible and can play a central role in functional genomics...
  16. ncbi request reprint High-throughput protein crystallization
    Raymond Hui
    Affinium Pharmaceuticals, 100 University Avenue, Ont, Toronto, Canada
    J Struct Biol 142:154-61. 2003
    ..Increasing the success rate will require a multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation...
  17. pmc A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    Nat Chem Biol 7:566-74. 2011
    ..In mouse embryonic stem cells, UNC0638 reactivated G9a-silenced genes and a retroviral reporter gene in a concentration-dependent manner without promoting differentiation...
  18. doi request reprint The precompetitive space: time to move the yardsticks
    Thea Norman
    Structural Genomics Consortium, University of Toronto, Toronto, ON M5G 1L7, Canada
    Sci Transl Med 3:76cm10. 2011
    ..The group will reconvene in April 2011 to create a business plan that specifies the generation of two positive POCM results per year...
  19. ncbi request reprint The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain
    E Bochkareva
    Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 273:3932-6. 1998
    ..The ssDNA-binding affinity of this trimeric complex was 3 to 5-fold higher than hRPA14.32-(43-171) alone, suggesting a role for the C terminus of hRPA70 in ssDNA binding...
  20. pmc Binding of elongin A or a von Hippel-Lindau peptide stabilizes the structure of yeast elongin C
    M V Botuyan
    Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9
    Proc Natl Acad Sci U S A 96:9033-8. 1999
    ..These results suggest a structural basis for the interaction of an F-box-containing protein with a SKP1 homologue and the modulation of this interaction by the tumor suppressor VHL...
  21. ncbi request reprint Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70
    V Booth
    Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 275:31266-8. 2000
    ....
  22. ncbi request reprint Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDP
    D Christendat
    Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 275:24608-12. 2000
    ..The conservation of the active site residues suggests that the mechanism of action is also conserved and that the RmlC structure may be useful in guiding the design of antibacterial drugs...
  23. ncbi request reprint Solution structure and dynamics of yeast elongin C in complex with a von Hippel-Lindau peptide
    M V Botuyan
    Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 2M9, Canada
    J Mol Biol 312:177-86. 2001
    ..Moreover, the disappearance of conformational exchange phenomena on the microsecond to millisecond time scale within Elc1 upon VHL peptide binding suggests a role for slow internal motions in ligand recognition...
  24. ncbi request reprint Elongin from Saccharomyces cerevisiae
    C M Koth
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8
    J Biol Chem 275:11174-80. 2000
    ..These studies revealed that yeast elongin A is unfolded but undergoes a dramatic modification of its structure in the presence of elongin C, and that elongin C forms a stable dimer in the absence of elongin A...
  25. ncbi request reprint Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation
    S M Orlicky
    Banting and Best Department of Medical Research and Department of Medical Genetics and Microbiology, C H Best Institute, University of Toronto, Ontario M5G 1L6, Canada
    J Biol Chem 276:10097-102. 2001
    ....
  26. ncbi request reprint Gene expression profiling of acute liver stress during living donor liver transplantation
    I Borozan
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    Am J Transplant 6:806-24. 2006
    ..This study has identified a set of genes whose expression is altered in acute, but not chronic, liver stress, likely to play a central role in the pathogenesis of acute liver injury of liver transplantation...
  27. ncbi request reprint RNA polymerase II subunit Rpb9 regulates transcription elongation in vivo
    S A Hemming
    Banting and Best Department of Medical Research, University of Toronto, Charles H Best Institute, Toronto, Ontario M5G 1L6, Canada
    J Biol Chem 275:35506-11. 2000
    ..Genome-wide transcription profiling of Deltarpb9 cells revealed only a few changes, predominantly in genes related to metabolism...