Darrell Desveaux

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. ncbi request reprint Type III effector proteins: doppelgangers of bacterial virulence
    Darrell Desveaux
    University of Toronto, 25 Willcocks Street, M5S 3B2, Toronto, Ontario, Canada
    Curr Opin Plant Biol 9:376-82. 2006
  2. doi request reprint A high-throughput chemical screen for resistance to Pseudomonas syringae in Arabidopsis
    Karl Schreiber
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, ON M5S 3B2, Canada
    Plant J 54:522-31. 2008
  3. pmc The HopZ family of Pseudomonas syringae type III effectors require myristoylation for virulence and avirulence functions in Arabidopsis thaliana
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, Ontario M5S 3B2, Canada
    J Bacteriol 190:2880-91. 2008
  4. pmc The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a
    Jennifer D Lewis
    Department of Cell and Systems Biology and Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON, Canada M5S 3B2
    Proc Natl Acad Sci U S A 110:18722-7. 2013
  5. pmc Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS Genet 6:e1000894. 2010
  6. pmc Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3B2, Canada
    BMC Genomics 13:8. 2012
  7. pmc Phytopathogen type III effectors as probes of biological systems
    Amy Huei Yi Lee
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Microb Biotechnol 6:230-40. 2013
  8. doi request reprint The targeting of plant cellular systems by injected type III effector proteins
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, ON M5S3B2, Canada
    Semin Cell Dev Biol 20:1055-63. 2009
  9. pmc A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion
    Amy Huei Yi Lee
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS Pathog 8:e1002523. 2012
  10. doi request reprint The YopJ superfamily in plant-associated bacteria
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada
    Mol Plant Pathol 12:928-37. 2011

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Type III effector proteins: doppelgangers of bacterial virulence
    Darrell Desveaux
    University of Toronto, 25 Willcocks Street, M5S 3B2, Toronto, Ontario, Canada
    Curr Opin Plant Biol 9:376-82. 2006
    ..Recent efforts to determine their three-dimensional structures are, however, revealing important clues about the mechanisms of bacterial virulence in plants...
  2. doi request reprint A high-throughput chemical screen for resistance to Pseudomonas syringae in Arabidopsis
    Karl Schreiber
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, ON M5S 3B2, Canada
    Plant J 54:522-31. 2008
    ..The whole-organism liquid assay provides a novel approach to probe chemical libraries in a high-throughput manner for compounds that reduce bacterial virulence in plants...
  3. pmc The HopZ family of Pseudomonas syringae type III effectors require myristoylation for virulence and avirulence functions in Arabidopsis thaliana
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, Ontario M5S 3B2, Canada
    J Bacteriol 190:2880-91. 2008
    ..This paper provides insight into the selective pressures driving virulence protein evolution by describing a detailed functional characterization of the diverse HopZ family of type III effectors with the model plant Arabidopsis...
  4. pmc The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a
    Jennifer D Lewis
    Department of Cell and Systems Biology and Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON, Canada M5S 3B2
    Proc Natl Acad Sci U S A 110:18722-7. 2013
    ..ZED1 is a nonfunctional kinase that forms part of small genomic cluster of kinases in Arabidopsis. We hypothesize that ZED1 acts as a decoy to lure HopZ1a to the ZAR1-resistance complex, resulting in ETI activation. ..
  5. pmc Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS Genet 6:e1000894. 2010
    ..Our results demonstrate that the Arabidopsis resistance protein ZAR1 confers allele-specific recognition and virulence attenuation of the Pseudomonas syringae T3SE protein HopZ1a...
  6. pmc Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3B2, Canada
    BMC Genomics 13:8. 2012
    ..Identification of protein-protein interactions is a fundamental aspect of understanding protein function. A commonly used method for identifying protein interactions is the yeast two-hybrid system...
  7. pmc Phytopathogen type III effectors as probes of biological systems
    Amy Huei Yi Lee
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Microb Biotechnol 6:230-40. 2013
    ..Furthermore, given that effectors directly and specifically interact with their targets within plant cells, these virulence proteins have enormous biotechnological potential for manipulating eukaryotic systems...
  8. doi request reprint The targeting of plant cellular systems by injected type III effector proteins
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, ON M5S3B2, Canada
    Semin Cell Dev Biol 20:1055-63. 2009
    ..Effector-host interactions reveal different stages of the battle between pathogen and host, as well as the diverse molecular strategies employed by bacterial pathogens to hijack eukaryotic cellular systems...
  9. pmc A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion
    Amy Huei Yi Lee
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS Pathog 8:e1002523. 2012
    ..Together, this study supports the hypothesis that HopZ1a promotes virulence through cytoskeletal and secretory disruption...
  10. doi request reprint The YopJ superfamily in plant-associated bacteria
    Jennifer D Lewis
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada
    Mol Plant Pathol 12:928-37. 2011
    ..The YopJ superfamily provides an excellent foundation for the study of effector diversification in the context of wide-ranging, co-evolutionary interactions...
  11. doi request reprint AlgW regulates multiple Pseudomonas syringae virulence strategies
    Karl J Schreiber
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada
    Mol Microbiol 80:364-77. 2011
    ..syringae required to dampen plant immune responses. Our findings support the conclusion that P. syringae co-ordinately regulates virulence strategies through AlgW in order to effectively suppress host immunity...
  12. pmc A high-throughput forward genetic screen identifies genes required for virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
    Karl J Schreiber
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 7:e41461. 2012
    ..A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth...
  13. pmc Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole
    Karl J Schreiber
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 3102, USA
    BMC Plant Biol 12:226. 2012
    ..These screens were based on the growth phenotype of seedlings germinated in the presence of the compound sulfamethoxazole (Smex)...
  14. doi request reprint Found in translation: high-throughput chemical screening in Arabidopsis thaliana identifies small molecules that reduce Fusarium head blight disease in wheat
    Karl J Schreiber
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Mol Plant Microbe Interact 24:640-8. 2011
    ..graminearum infection in wheat. As such, the Arabidopsis-based liquid assay represents a biologically relevant surrogate system for high-throughput studies of agriculturally important plant-pathogen interactions...
  15. ncbi request reprint A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis
    Shelley Lumba
    Cell and Systems Biology, University of Toronto and the Centre for The Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S 3B2, Canada
    Dev Cell 29:360-72. 2014
    ..This comprehensive ABA resource allows for application of approaches to understanding ABA functions in higher plants. ..
  16. doi request reprint The roles of ABA in plant-pathogen interactions
    Feng Yi Cao
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada
    J Plant Res 124:489-99. 2011
    ....
  17. doi request reprint Next-generation genomics of Pseudomonas syringae
    Heath E O'Brien
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada
    Curr Opin Microbiol 14:24-30. 2011
    ..In general, these discoveries have illustrated the utility of draft genome sequencing for quickly and economically identifying candidate loci for more refined genetic and functional analyses...
  18. pmc The type III effector HopF2Pto targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence
    Mike Wilton
    Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada
    Proc Natl Acad Sci U S A 107:2349-54. 2010
    ..This virulence activity was not observed in plants genetically lacking RIN4. These data provide evidence that RIN4 is a major virulence target of HopF2(Pto) and that a pathogenic advantage can be conveyed by TTSEs that target RIN4...
  19. pmc Peptide binding properties of the three PDZ domains of Bazooka (Drosophila Par-3)
    Cao Guo Yu
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 9:e86412. 2014
    ..Thus, the peptide binding pockets of each PDZ domain in Baz are not obviously affected by the presence of neighbouring PDZ domains, but act as isolated modules with specific in vitro peptide binding preferences. ..
  20. doi request reprint Plant chemical genetics
    Peter McCourt
    Department of Cell and Systems Biology, University of Toronto, 25 Willcocks St, Toronto, ON, Canada
    New Phytol 185:15-26. 2010
    ..Because chemical genetics is rooted in genetic analysis, we focus on how chemicals used in combination with genetics can be very powerful in dissecting a process of interest...
  21. pmc Lessons learned from type III effector transgenic plants
    Mike Wilton
    Department of Cell and Systems Biology, University of Toronto, Toronto, Canada
    Plant Signal Behav 5:746-8. 2010
    ..syringae TTSEs. As we describe here, transgenic Arabidopsis plants expressing individual TTSEs have also be used to overcome problems of functional redundancy and provide invaluable insights into TTSE virulence functions...
  22. ncbi request reprint Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis
    Alex U Singer
    Department of Pharmacology, University of North Carolina at Chapel Hill, 27599, USA
    Structure 12:1669-81. 2004
    ....
  23. pmc The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally acylated target, RIN4, from Arabidopsis membranes to block RPM1 activation
    Han Suk Kim
    Department of Biology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 102:6496-501. 2005
    ....
  24. pmc Type III effector activation via nucleotide binding, phosphorylation, and host target interaction
    Darrell Desveaux
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Pathog 3:e48. 2007
    ..Our data suggest that activated AvrB, bound to RIN4, is indirectly recognized by RPM1 to initiate plant immune system function...
  25. ncbi request reprint The HopX (AvrPphE) family of Pseudomonas syringae type III effectors require a catalytic triad and a novel N-terminal domain for function
    ZACHARY L NIMCHUK
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Plant Microbe Interact 20:346-57. 2007
    ....
  26. pmc The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1
    Charles Despres
    Department of Biological Sciences, Brock University, St Catharines, Ontario, Canada L2S 3A1
    Plant Cell 15:2181-91. 2003
    ..Unlike its animal and yeast counterparts, the DNA binding activity of TGA1 is not redox regulated; however, this property is conferred by interaction with the NPR1 cofactor...