J R Davie

Summary

Affiliation: University of Manitoba
Country: Canada

Publications

  1. ncbi Ser-10 phosphorylation of histone H3 and immediate early gene expression in oncogene-transformed mouse fibroblasts
    Ileana S Strelkov
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba, R3E 0V9 Canada
    Cancer Res 62:75-8. 2002
  2. ncbi Histone modifications as a platform for cancer therapy
    Paula S Espino
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9, Canada
    J Cell Biochem 94:1088-102. 2005
  3. pmc Phosphorylated serine 28 of histone H3 is associated with destabilized nucleosomes in transcribed chromatin
    Jian Min Sun
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9 Canada
    Nucleic Acids Res 35:6640-7. 2007
  4. pmc RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing
    Dilshad H Khan
    Department of Biochemistry and Medical Genetics, University of Manitoba, Manitoba Institute of Child Health, Winnipeg, Manitoba, R3E 3P4, Canada, Department of Biochemistry and Medical Genetics, University of Manitoba, Manitoba Institute of Cell Biology, Winnipeg, Manitoba, R3E0V9, Canada and Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nucleic Acids Res 42:1656-70. 2014
  5. pmc Mitogen- and stress-activated protein kinases 1 and 2 are required for maximal trefoil factor 1 induction
    Protiti Khan
    Manitoba Institute of Child Health, University of Manitoba, Winnipeg, Manitoba, Canada
    PLoS ONE 8:e63189. 2013
  6. pmc Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells
    Xuemei Wang
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E0V9, Canada
    Mol Biol Cell 21:2987-95. 2010
  7. doi Targeting class I histone deacetylases in cancer therapy
    Geneviève P Delcuve
    University of Manitoba, Manitoba Institute of Child Health, 715 McDermot Avenue, Room 600A, Winnipeg, Manitoba, R3E 3P4, Canada
    Expert Opin Ther Targets 17:29-41. 2013
  8. pmc Roles of histone deacetylases in epigenetic regulation: emerging paradigms from studies with inhibitors
    Geneviève P Delcuve
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, R3E 0V9, Canada
    Clin Epigenetics 4:5. 2012
  9. ncbi Control of histone modifications
    J R Davie
    Manitoba Institute of Cell Biology and the Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3E 0V9 Canada
    J Cell Biochem . 1999
  10. ncbi Organization of chromatin in cancer cells: role of signalling pathways
    J R Davie
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada
    Biochem Cell Biol 77:265-75. 1999

Collaborators

Detail Information

Publications67

  1. ncbi Ser-10 phosphorylation of histone H3 and immediate early gene expression in oncogene-transformed mouse fibroblasts
    Ileana S Strelkov
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba, R3E 0V9 Canada
    Cancer Res 62:75-8. 2002
    ..We propose that persistent activation of the Ras-MAPK pathway and MSK1 resulting in the elevation of phosphorylated H3 levels may contribute to the aberrant gene expression observed in the oncogene-transformed cells...
  2. ncbi Histone modifications as a platform for cancer therapy
    Paula S Espino
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9, Canada
    J Cell Biochem 94:1088-102. 2005
    ..This article will review the potential of these reversible epigenetic processes as targets for cancer therapies...
  3. pmc Phosphorylated serine 28 of histone H3 is associated with destabilized nucleosomes in transcribed chromatin
    Jian Min Sun
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9 Canada
    Nucleic Acids Res 35:6640-7. 2007
    ..3 phosphorylated at serine 28 was present in labile nucleosomes. We propose that destabilized nucleosomes containing H3.3 phosphorylated at serine 28 aid in the dynamic disassembly-assembly of nucleosomes in active promoters...
  4. pmc RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing
    Dilshad H Khan
    Department of Biochemistry and Medical Genetics, University of Manitoba, Manitoba Institute of Child Health, Winnipeg, Manitoba, R3E 3P4, Canada, Department of Biochemistry and Medical Genetics, University of Manitoba, Manitoba Institute of Cell Biology, Winnipeg, Manitoba, R3E0V9, Canada and Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Nucleic Acids Res 42:1656-70. 2014
    ..Thus, nonphosphorylated HDAC1/2 is recruited to pre-mRNA by splicing factors to act at the RNA level with KAT2B and other KATs to catalyze dynamic histone acetylation of the MCL1 alternative exon and alter the splicing of MCL1 pre-mRNA. ..
  5. pmc Mitogen- and stress-activated protein kinases 1 and 2 are required for maximal trefoil factor 1 induction
    Protiti Khan
    Manitoba Institute of Child Health, University of Manitoba, Winnipeg, Manitoba, Canada
    PLoS ONE 8:e63189. 2013
    ..Moreover, we show that MSK1 or MSK2 is recruited to TFF1 regulatory regions, but as components of different multiprotein complexes...
  6. pmc Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells
    Xuemei Wang
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E0V9, Canada
    Mol Biol Cell 21:2987-95. 2010
    ..Because PRDX1 phosphorylation is known to inhibit its peroxidase activity and to promote PRDX1 oligomerization, we propose that PRDX1 acts as a chaperone to enhance the transactivation potential of NF-kappaB in ER- breast cancer cells...
  7. doi Targeting class I histone deacetylases in cancer therapy
    Geneviève P Delcuve
    University of Manitoba, Manitoba Institute of Child Health, 715 McDermot Avenue, Room 600A, Winnipeg, Manitoba, R3E 3P4, Canada
    Expert Opin Ther Targets 17:29-41. 2013
    ..Hence, the field of cancer therapy has experienced a continued surge in the development of HDAC inhibitors...
  8. pmc Roles of histone deacetylases in epigenetic regulation: emerging paradigms from studies with inhibitors
    Geneviève P Delcuve
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, R3E 0V9, Canada
    Clin Epigenetics 4:5. 2012
    ..HDAC inhibitors might also regulate transcription elongation and have been shown to impinge on alternative splicing...
  9. ncbi Control of histone modifications
    J R Davie
    Manitoba Institute of Cell Biology and the Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3E 0V9 Canada
    J Cell Biochem . 1999
    ..A new and exciting development, presented in this review, is the role of signal transduction pathways in the phosphorylation of histone H3 and the expression of immediate-early genes. J. Cell. Biochem. Suppls. 32/33:141-148, 1999...
  10. ncbi Organization of chromatin in cancer cells: role of signalling pathways
    J R Davie
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada
    Biochem Cell Biol 77:265-75. 1999
    ..Histone phosphorylation may lead to decondensation of chromatin, resulting in aberrant gene expression...
  11. ncbi MSK1 and MSK2 mediate mitogen- and stress-induced phosphorylation of histone H3: a controversy resolved
    James R Davie
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, Manitoba, Canada, R3E 0V9
    Sci STKE 2003:PE33. 2003
    ....
  12. ncbi Regulation and regulatory parameters of histone modifications
    J R Davie
    Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada
    J Cell Biochem Suppl 30:203-13. 1998
    ....
  13. ncbi Covalent modifications of histones: expression from chromatin templates
    J R Davie
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Curr Opin Genet Dev 8:173-8. 1998
    ..The recruited enzymes may either acetylate or deacetylate proximal nucleosomal histones or nonhistone chromosomal proteins...
  14. ncbi Inhibition of histone deacetylase activity by butyrate
    James R Davie
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba, Canada
    J Nutr 133:2485S-2493S. 2003
    ..Pending the cell background, the nonproliferating cells may enter differentiation or apoptotic pathways. The potential of butyrate and HDAC inhibitors in the prevention and treatment of cancer is presented...
  15. ncbi Effect of estradiol on histone acetylation dynamics in human breast cancer cells
    J M Sun
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 276:49435-42. 2001
    ....
  16. ncbi Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts
    D N Chadee
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9 Canada
    J Biol Chem 274:24914-20. 1999
    ..Elevated levels of phosphorylated H1 and H3 may be responsible for the less condensed chromatin structure and aberrant gene expression observed in the oncogene-transformed cells...
  17. pmc Effects of histone acetylation, ubiquitination and variants on nucleosome stability
    W Li
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Biochem J 296:737-44. 1993
    ..The elevated lability of the H2A-H2B dimer may facilitate processes such as the exchange of these dimers with newly synthesized histones, the elongation process of transcription and transcription factor binding...
  18. ncbi Histone H1b phosphorylation is dependent upon ongoing transcription and replication in normal and ras-transformed mouse fibroblasts
    D N Chadee
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 272:8113-6. 1997
    ..Phosphorylation of H1b may be required to maintain a more decondensed chromatin structure to facilitate transcription and replication processes...
  19. ncbi Purification and characterization of chicken erythrocyte histone deacetylase 1
    J M Sun
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Biochemistry 38:5939-47. 1999
    ..We propose that in addition to catalyzing dynamic acetylation of transcribed chromatin, the enzyme has a role in the organization of nuclear DNA...
  20. ncbi Potential role of estrogen receptor alpha (ERalpha) phosphorylated at Serine118 in human breast cancer in vivo
    Leigh C Murphy
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Man R3E 0V9, Canada
    J Steroid Biochem Mol Biol 102:139-46. 2006
    ..However, the in vivo data are somewhat contradictory and limited by the small cohorts used and the lack of standard well-characterized reagents and protocols...
  21. ncbi Dynamically acetylated histone association with transcriptionally active and competent genes in the avian adult beta-globin gene domain
    V A Spencer
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 276:34810-5. 2001
    ..Our observations are consistent with a model in which nuclear matrix-associated histone acetyltransferases and deacetylases mediate a dynamic attachment between active and competent chromatin and the nuclear matrix...
  22. ncbi CUG-initiated FGF-2 induces chromatin compaction in cultured cardiac myocytes and in vitro
    G Sun
    Department of Human Anatomy and Cell Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    J Cell Physiol 186:457-67. 2001
    ..By affecting chromatin compaction "hi" FGF-2 may contribute to the regulation of gene expression...
  23. ncbi Chromatin modification of the trefoil factor 1 gene in human breast cancer cells by the Ras/mitogen-activated protein kinase pathway
    Paula S Espino
    Manitoba Institute of Cell Biology, Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
    Cancer Res 66:4610-6. 2006
    ..These results show that although both stimuli lead to TFF1 gene activation, estradiol and TPA exert their effects on TFF1 gene expression by different mechanisms...
  24. ncbi Altered profiles in nuclear matrix proteins associated with DNA in situ during progression of breast cancer cells
    V A Spencer
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada
    Cancer Res 61:1362-6. 2001
    ..Our results suggest that progression of breast cancer is accompanied by a reorganization of chromosomal domains, which may lead to alterations in gene expression...
  25. ncbi Ubiquitinated histone H2B is preferentially located in transcriptionally active chromatin
    B E Nickel
    Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Biochemistry 28:958-63. 1989
    ..These observations are consistent with the hypothesis that ubiquitinated histones have a role maintaining the structure of transcriptionally active chromatin...
  26. ncbi Ubiquitination of histone H3 in elongating spermatids of rat testes
    H Y Chen
    Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0W3 Canada
    J Biol Chem 273:13165-9. 1998
    ..We speculate that its ubiquitination could loosen the nucleosome structure in preparation for histone removal, be a consequence of nucleosome relaxation or disruption caused by other means, or target H3 for degradation...
  27. pmc An integrated analysis of genes and pathways exhibiting metabolic differences between estrogen receptor positive breast cancer cells
    Soma Mandal
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg Manitoba, R3E 0V9, Canada
    BMC Cancer 7:181. 2007
    ..The sex hormone estrogen (E2) is pivotal to normal mammary gland growth and differentiation and in breast carcinogenesis. In this in silico study, we examined metabolic differences between ER(+)ve breast cancer cells during E2 deprivation...
  28. ncbi Estrogen receptor-alpha phosphorylated at Ser118 is present at the promoters of estrogen-regulated genes and is not altered due to HER-2 overexpression
    Gregory E Weitsman
    Department of Biochemistry and Medical Genetics and Department of Pathology, Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    Cancer Res 66:10162-70. 2006
    ....
  29. ncbi Isolation of transcriptionally active chromatin from human breast cancer cells using Sulfolink coupling gel chromatography
    Jian Min Sun
    Manitoba Institute of Cell Biology, 675 McDermot Avenue, Winnipeg, Manitoba, R3E 0V9, Canada
    J Cell Biochem 84:439-46. 2002
    ..We conclude that in cultured T5 breast cancer cells H3 and H4 are in highly acetylated states maintaining the unfolded structure of the transcribed nucleosome and facilitating subsequent rounds of elongation...
  30. ncbi Inducible upregulation of oestrogen receptor-beta1 affects oestrogen and tamoxifen responsiveness in MCF7 human breast cancer cells
    L C Murphy
    Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
    J Mol Endocrinol 34:553-66. 2005
    ..These models are relevant and will be useful for dissecting the role of ERbeta1 expression in ER-positive breast cancer...
  31. doi Estrogen regulated expression of the p21 Waf1/Cip1 gene in estrogen receptor positive human breast cancer cells
    Soma Mandal
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    J Cell Physiol 224:28-32. 2010
    ..Although Sp1/Sp3 is not involved in the recruitment of ER alpha to the promoter, Sp1 is necessary for estrogen-induced p21(Waf1/Cip1) promoter activity...
  32. doi Genomic instability and histone H3 phosphorylation induction by the Ras-mitogen activated protein kinase pathway in pancreatic cancer cells
    Paula S Espino
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    Int J Cancer 124:562-7. 2009
    ..Our results present evidence that nonclonal chromosomal aberrations and epigenetic programming responding to stimulation of the Ras-MAPK pathway may be better markers for cancer progression than the upstream mutated oncogenes...
  33. ncbi Histone H1(S)-3 phosphorylation in Ha-ras oncogene-transformed mouse fibroblasts
    Deborah N Chadee
    Manitoba Institute of Cell Biology, 675 McDermot Avenue, Winnipeg, Manitoba, R3E 0V9 Canada
    Oncogene 21:8397-403. 2002
    ..The chromatin remodelling actions of these modified histones may result in aberrant gene expression...
  34. ncbi Mitogen- and stress-activated protein kinase 1 activity and histone h3 phosphorylation in oncogene-transformed mouse fibroblasts
    Bojan Drobic
    Manitoba Institute of Cell Biology, Department of Biochemistry, University of Manitoba, Winnipeg, Manitoba, Canada
    Cancer Res 64:9076-9. 2004
    ....
  35. ncbi Estrogen regulation of trefoil factor 1 expression by estrogen receptor alpha and Sp proteins
    Jian Min Sun
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9, Canada
    Exp Cell Res 302:96-107. 2005
    ....
  36. ncbi The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2
    Jian Min Sun
    Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    J Biol Chem 277:35783-6. 2002
    ..CK2 phosphorylation of HDAC2 recruited by Sp1 or Sp3 could regulate HDAC activity and alter the balance of histone deacetylase and histone acetyltransferase activities and dynamic chromatin remodeling of estrogen-regulated genes...
  37. ncbi Characterization of stably transfected fusion protein GFP-estrogen receptor-alpha in MCF-7 human breast cancer cells
    Helen Zhao
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, Canada R3E 0V9
    J Cell Biochem 86:365-75. 2002
    ..Furthermore, GFP-ER expression did not affect the estradiol induced temporal expression of hormone responsive genes c-myc and pS2...
  38. pmc Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex
    Bojan Drobic
    Department of Immunology, University of Manitoba, Manitoba Institute of Cell Biology, Winnipeg, Manitoba R3E 0V9, Canada
    Nucleic Acids Res 38:3196-208. 2010
    ..The recruited SWI/SNF remodels nucleosomes at the promoter of IE genes enabling the binding of transcription factors like JUN and the onset of transcription...
  39. ncbi Competitive inhibition of histone deacetylase activity by trichostatin A and butyrate
    Anoushe Sekhavat
    MB Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, Manitoba, Canada
    Biochem Cell Biol 85:751-8. 2007
    ..Thus, repressor complexes such as Sin3, appear to be stable in the presence of TSA. The association of HDAC2 with transcription factor Sp1 is also not affected by TSA...
  40. ncbi Chromatin immunoprecipitation: a tool for studying histone acetylation and transcription factor binding
    Virginia A Spencer
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
    Methods 31:67-75. 2003
    ..In this article, we present the ChIP assay currently used in our lab and discuss the various ways to optimize this assay for one's own use...
  41. ncbi Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28
    Katherine L Dunn
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, Manitoba, Canada R3E 0V9
    Oncogene 24:3492-502. 2005
    ....
  42. ncbi Measurement of histone acetyltransferase and histone deacetylase activities and kinetics of histone acetylation
    Jian Min Sun
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Man, Winnipeg, Canada R3E 0V9
    Methods 31:12-23. 2003
    ..Procedures to assay the activities of these enzymes and to measure the kinetics of histone acetylation and deacetylation are presented...
  43. pmc Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin
    A M Spence
    Department of Physiology, University of Manitoba, Winnipeg, Canada
    Proc Natl Acad Sci U S A 86:7843-7. 1989
    ..These data are consistent with a mechanism of translational regulation of a eukaryotic bifunctional mRNA...
  44. ncbi Expression of rainbow trout apolipoprotein A-I genes in liver and hepatocellular carcinoma
    G P Delcuve
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    J Lipid Res 33:251-62. 1992
    ..The similarity of the repeat pattern in trout and human apoA-Is suggests that all the internal repeats in these sequences arose before the fish-mammal split, some 400 million years ago...
  45. doi Increased genomic instability and altered chromosomal protein phosphorylation timing in HRAS-transformed mouse fibroblasts
    Katherine L Dunn
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
    Genes Chromosomes Cancer 48:397-409. 2009
    ..Together our results demonstrate the increased genomic instability and alterations in the epigenetic program in HRAS-transformed cells...
  46. ncbi Epigenetic control
    Geneviève P Delcuve
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    J Cell Physiol 219:243-50. 2009
    ..For example, recent studies challenge the idea that DNA methylation is irreversible. Further, research on Rett syndrome has revealed an unforeseen role for methyl-CpG-binding protein 2 (MeCP2) in neurons...
  47. doi Association of Sp3 and estrogen receptor alpha with the transcriptionally active trefoil factor 1 promoter in MCF-7 breast cancer cells
    Lin Li
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    J Cell Biochem 105:365-9. 2008
    ..Taken together, these results provide evidence that Sp3 and ERalpha are involved in the estrogen induced transcription of the TFF1 gene...
  48. ncbi The estrogen receptor: more than the average transcription factor
    Laura L Hart
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada
    Biochem Cell Biol 80:335-41. 2002
    ..The resulting levels of estrogen receptor and the type of ligand bound to it have a direct impact on the transcription of estrogen-responsive genes...
  49. ncbi Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin
    Jian Min Sun
    Manitoba Institute of Cell Biology, University of Manitoba, 675 McDermot Avenue, Winnipeg, Manitoba, Canada
    J Biol Chem 282:33227-36. 2007
    ..We provide evidence that unmodified HDAC2 is associated with the coding region of transcribed genes, whereas phosphorylated HDAC2 is primarily recruited to promoters...
  50. doi H3 phosphorylation: dual role in mitosis and interphase
    Beatriz Pérez-Cadahía
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, MB R3E0V9, Canada
    Biochem Cell Biol 87:695-709. 2009
    ..The cellular contexts under which specific residues on H3 are phosphorylated in mitosis and interphase are known to some extent. However, the functional consequences of H3 phosphorylation are still unclear...
  51. ncbi Gene regulation by Sp1 and Sp3
    Lin Li
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada
    Biochem Cell Biol 82:460-71. 2004
    ..Regulation of the transcriptional activity of Sp1 and Sp3 occurs largely at the post-translational level. In this review, we focus on the roles of Sp1 and Sp3 in the regulation of gene expression...
  52. ncbi Elevated expression of the estrogen receptor prevents the down-regulation of p21Waf1/Cip1 in hormone dependent breast cancer cells
    Helen Zhao
    Manitoba Institute of Cell Biology, 675 McDermot Avenue, Winnipeg, Manitoba, Canada R3E 0V9
    J Cell Biochem 93:619-28. 2004
    ..The results show that elevated levels of ER prevented the down-regulation of p21 protein expression, which is required for hormone responsive cells to enter S phase...
  53. ncbi Estrogen receptor-beta regulates psoriasin (S100A7) in human breast cancer
    George P Skliris
    Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada
    Breast Cancer Res Treat 104:75-85. 2007
    ..These data support the hypothesis that psoriasin/S100A7 is specifically regulated by ERbeta activity and could be useful to guide future therapies targeting ERbeta in certain phenotypic subsets of breast cancers in-vivo...
  54. ncbi Phosphorylation of histones by tissue transglutaminase
    Suresh Mishra
    Department of Physiology, University of Manitoba, Winnipeg, Manitoba R3E 0W3, Canada
    J Biol Chem 281:5532-8. 2006
    ..These studies suggest a novel role for TG2 in the regulation of chromatin structure and function...
  55. ncbi The insulator binding protein CTCF associates with the nuclear matrix
    Katherine L Dunn
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    Exp Cell Res 288:218-23. 2003
    ..We propose that CTCF may demarcate nuclear matrix-dependent points of transition in chromatin, thereby forming topologically independent chromatin loops that may support gene silencing...
  56. pmc Differential intranuclear organization of transcription factors Sp1 and Sp3
    Shihua He
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    Mol Biol Cell 16:4073-83. 2005
    ..Our results demonstrate the different features of Sp1 and Sp3, providing further evidence that Sp3 is not a functional equivalent of Sp1...
  57. doi The role of Sp1 and Sp3 in normal and cancer cell biology
    Lin Li
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    Ann Anat 192:275-83. 2010
    ..In this review, the role of Sp1 and Sp3 in normal and cancer cell biology and the multiple mechanisms deciding the functional roles of Sp1 and Sp3 will be presented...
  58. ncbi Chromatin organization and nuclear microenvironments in cancer cells
    Shihua He
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada
    J Cell Biochem 104:2004-15. 2008
    ..This article will review genetic and epigenetic events that alter chromatin organization in cancer cells and the role of the nuclear matrix in determining nuclear morphology...
  59. ncbi Sp1 and Sp3 foci distribution throughout mitosis
    Shihua He
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9, Canada
    J Cell Sci 119:1063-70. 2006
    ..Both Sp1 and Sp3 return to the nuclei ahead of RNA polymerase II. Our results support a model in which entry of Sp1, Sp3 and RNA polymerase II into the newly formed nuclei is an ordered process...
  60. ncbi Mitotic partitioning of transcription factors
    Geneviève P Delcuve
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9
    J Cell Biochem 105:1-8. 2008
    ..The elucidation of how the memory of a programmed expression profile is transmitted to daughter cells represents a challenge...
  61. ncbi Transcriptional silencing of the death gene BNIP3 by cooperative action of NF-kappaB and histone deacetylase 1 in ventricular myocytes
    James Shaw
    Institute of Cardiovascular Sciences, St Boniface General Hospital Research Centre Rm 3016, 351 Tache Avenue, Winnipeg, Manitoba, Canada, R2H 2A6
    Circ Res 99:1347-54. 2006
    ....
  62. pmc Effects of the in vivo supply of butyrate on histone acetylation of cecum in piglets
    C Lawrence Kien
    Department of Pediatrics, College of Medicine, University of Vermont, Burlington, Vermont 05405, USA
    JPEN J Parenter Enteral Nutr 32:51-6. 2008
    ..In vitro, butyrate inhibits histone deacetylase and down-regulates expression of cyclin D1. We hypothesized that an increased entry rate of butyrate into the cecal lumen would have similar effects in vivo...
  63. ncbi Histone H4-K16 acetylation controls chromatin structure and protein interactions
    Michael Shogren-Knaak
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 311:844-7. 2006
    ....
  64. ncbi The role of Sp1 and Sp3 in the constitutive DPYD gene expression
    Xue Zhang
    Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochim Biophys Acta 1759:247-56. 2006
    ..These studies provide further insight into the molecular mechanisms underlying the heterogeneity of DPD activity, and may facilitate the efficacy and safety of 5-FU-based chemotherapy...
  65. ncbi NAPP2, a peroxisomal membrane protein, is also a transcriptional corepressor
    Narender R Gavva
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China
    Genomics 79:423-31. 2002
    ..They also suggest that NF-E2 may function both positively and negatively in the transcription regulation of specific erythroid and megakaryocytic genes...
  66. ncbi Suppression of DPYD expression in RKO cells via DNA methylation in the regulatory region of the DPYD promoter: a potentially important epigenetic mechanism regulating DPYD expression
    Xue Zhang
    Mayo Clinic Cancer Center, Mayo Clinic, 200 First Street, SW, Rochester, MN 55905, USA
    Biochem Cell Biol 85:337-46. 2007
    ..This may also provide insight into the mechanism underlying the downregulation of DPYD in some colorectal cancers...
  67. ncbi CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins
    Helen H Tai
    Ottawa Regional Cancer Centre, and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ont, Canada
    Biochem Biophys Res Commun 308:170-6. 2003
    ..Splicing assays show that CHD1 overexpression can affect alternative splicing. These results suggest that CHD1 may function in both chromatin mediated transcriptional repression and RNA splicing...