F Y Choy

Summary

Affiliation: University of Victoria
Country: Canada

Publications

  1. ncbi Secretion of human glucocerebrosidase from stable transformed insect cells using native signal sequences
    Graham Sinclair
    Biomedical Research Centre, Department of Biology, University of Victoria, Station CSC, Canada
    Biochem Cell Biol 84:148-56. 2006
  2. ncbi Gaucher disease and the synucleinopathies: refining the relationship
    Tessa N Campbell
    TNC Scientific Consulting, Calgary, AB, Canada
    Orphanet J Rare Dis 7:12. 2012
  3. ncbi Gaucher disease among Chinese patients: review on genotype/phenotype correlation from 29 patients and identification of novel and rare alleles
    Francis Y M Choy
    Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, Canada
    Blood Cells Mol Dis 38:287-93. 2007
  4. ncbi Glycine cleavage enzyme complex: rabbit H-protein cDNA sequence analysis and comparison to human, cow, and chicken
    F Choy
    Department of Biology, Centre for Environmental Health, University of Victoria, BC, Canada
    Biochem Cell Biol 78:725-30. 2000
  5. ncbi A novel mutation (V191G) in a German-British type 1 Gaucher disease patient. Mutations in brief no. 131. Online
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, Canada
    Hum Mutat 11:411-2. 1998
  6. ncbi Identification of two novel and four uncommon missense mutations among chinese Gaucher disease patients
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, B C, Canada
    Am J Med Genet 71:172-8. 1997
  7. ncbi Heterologous expression and characterization of a rare Gaucher disease mutation (c.481C > T) from a Canadian aboriginal population using archival tissue samples
    G Sinclair
    Department of Biology, Centre for Environmental Health, University of Victoria, Victoria, British Columbia, Canada
    Mol Genet Metab 74:345-52. 2001
  8. ncbi DNA analysis of an uncommon missense mutation in a Gaucher disease patient of Jewish-Polish-Russian descent
    F Y Choy
    Department of Biology, University of Victoria, British Columbia, Canada
    Am J Med Genet 51:156-60. 1994
  9. ncbi Gaucher disease: functional expression of the normal glucocerebrosidase and Gaucher T1366G and G1604A alleles in Baculovirus-transfected Spodoptera frugiperda cells
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, Canada
    Am J Med Genet 65:184-9. 1996
  10. ncbi A novel complex allele and two new point mutations in type 2 (acute neuronopathic) Gaucher disease
    G Sinclair
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, V8W 3N6, Canada
    Blood Cells Mol Dis 24:420-7. 1998

Collaborators

Detail Information

Publications22

  1. ncbi Secretion of human glucocerebrosidase from stable transformed insect cells using native signal sequences
    Graham Sinclair
    Biomedical Research Centre, Department of Biology, University of Victoria, Station CSC, Canada
    Biochem Cell Biol 84:148-56. 2006
    ....
  2. ncbi Gaucher disease and the synucleinopathies: refining the relationship
    Tessa N Campbell
    TNC Scientific Consulting, Calgary, AB, Canada
    Orphanet J Rare Dis 7:12. 2012
    ..Possible mechanisms of interaction will be discussed...
  3. ncbi Gaucher disease among Chinese patients: review on genotype/phenotype correlation from 29 patients and identification of novel and rare alleles
    Francis Y M Choy
    Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, Canada
    Blood Cells Mol Dis 38:287-93. 2007
    ..With the exception of N370S and R353W found only in the Type 1 form, the majority of these mutations are severe ones that result in poor prognosis and often Types 2 and 3 Gaucher disease...
  4. ncbi Glycine cleavage enzyme complex: rabbit H-protein cDNA sequence analysis and comparison to human, cow, and chicken
    F Choy
    Department of Biology, Centre for Environmental Health, University of Victoria, BC, Canada
    Biochem Cell Biol 78:725-30. 2000
    ..We have isolated the full-length cDNA of the H-protein gene from the rabbit (Oryctolagus caniculus) by reverse transcription of liver poly-A mRNA and determined its nucleotide sequence (GenBank Acc. No. BankIt 31828..
  5. ncbi A novel mutation (V191G) in a German-British type 1 Gaucher disease patient. Mutations in brief no. 131. Online
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, Canada
    Hum Mutat 11:411-2. 1998
    ....
  6. ncbi Identification of two novel and four uncommon missense mutations among chinese Gaucher disease patients
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, B C, Canada
    Am J Med Genet 71:172-8. 1997
    ..The population genetics of some of these Gaucher alleles and their implications in genotype/phenotype correlation are discussed...
  7. ncbi Heterologous expression and characterization of a rare Gaucher disease mutation (c.481C > T) from a Canadian aboriginal population using archival tissue samples
    G Sinclair
    Department of Biology, Centre for Environmental Health, University of Victoria, Victoria, British Columbia, Canada
    Mol Genet Metab 74:345-52. 2001
    ..This technique will be applicable to the screening of this and other populations for the frequency of known Gaucher mutations where traditional DNA sources are unavailable...
  8. ncbi DNA analysis of an uncommon missense mutation in a Gaucher disease patient of Jewish-Polish-Russian descent
    F Y Choy
    Department of Biology, University of Victoria, British Columbia, Canada
    Am J Med Genet 51:156-60. 1994
    ..abstract truncated at 250 words)..
  9. ncbi Gaucher disease: functional expression of the normal glucocerebrosidase and Gaucher T1366G and G1604A alleles in Baculovirus-transfected Spodoptera frugiperda cells
    F Y Choy
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, Canada
    Am J Med Genet 65:184-9. 1996
    ..Moreover, the use of this expression system demonstrates that the G1604A and T1366G mutations are both deleterious mutations resulting in profoundly deficient glucocerebrosidase activity and subsequent Gaucher disease...
  10. ncbi A novel complex allele and two new point mutations in type 2 (acute neuronopathic) Gaucher disease
    G Sinclair
    Centre for Environmental Health, Department of Biology, University of Victoria, British Columbia, V8W 3N6, Canada
    Blood Cells Mol Dis 24:420-7. 1998
    ....
  11. ncbi RNA interference: past, present and future
    Tessa N Campbell
    Dept of Biology, Centre for Biomedical Research, University of Victoria, BC V8W 3N5 Canada
    Curr Issues Mol Biol 7:1-6. 2005
    ..In this review, we discuss the landmark discoveries that established the contextual framework leading up to our current understanding of RNAi. We also provide an overview of current developments and future applications...
  12. ncbi Knockdown of chimeric glucocerebrosidase by green fluorescent protein-directed small interfering RNA
    Tessa N Campbell
    Centre for Biomedical Research Department of Biology, University of Victoria, Victoria, BC, Canada
    Genet Mol Res 3:282-7. 2004
    ..Furthermore, this technique is applicable to other systems, since we have demonstrated the usefulness of GFP as a siRNA target in mammalian cells when fused to another gene of interest...
  13. ncbi Expression of two green fluorescent protein variants in citrate-buffered media in Pichia pastoris
    Tessa N Campbell
    Department of Biology, Centre for Biomedical Research, University of Victoria, Box 3020 STN CSC, Victoria, BC, Canada V8W 3N5
    Anal Biochem 311:193-5. 2002
  14. ncbi Approaches to library screening
    Tessa N Campbell
    Center for Biomedical Research, Department of Biology, University of Victoria, British Columbia, Canada
    J Mol Microbiol Biotechnol 4:551-4. 2002
    ..Multi-faceted approaches combining different techniques, as well as less frequently employed methods such as fingerprinting are also described...
  15. ncbi Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris
    Graham Sinclair
    Department of Biology, Centre for Biomedical Research, University of Victoria, P O Box 3020 STN CSC, BC, V8W 3N5, Victoria, Canada
    Protein Expr Purif 26:96-105. 2002
    ..6- and 7.5-fold, respectively. Optimized G+C content, regardless of corresponding codon optimization, appears to be the major contributor to increased translational efficiency in this heterologous expression host...
  16. ncbi Identification of a new mutation (P178S) in an African-American patient with type 2 Gaucher disease
    F Y Choy
    Department of Biology, University of Victoria, British Columbia, Canada
    Hum Mutat 5:345-7. 1995
  17. ncbi HIV TAT variants differentially influence the production of glucocerebrosidase in Sf9 cells
    Andrea K Vaags
    Department of Biology, University of Victoria, Victoria, BC, Canada
    Genet Mol Res 4:491-5. 2005
    ..All three constructs exhibited successful expression, with wild-type TAT chimeras showing lower levels of expression than modified TAT chimeras...
  18. ncbi Glucocerebrosidase recombinant allele: molecular evolution of the glucocerebrosidase gene and pseudogene in primates
    Julie R Wafaei
    Centre for Biomedical Research, Department of Biology, University of Victoria, P.O. Box 3020, Station CSC, Victoria, BC, Canada V8W 3N5
    Blood Cells Mol Dis 35:277-85. 2005
    ..Phylogenetic tree analysis using DNAstar and PAUP4.0 software indicates that gene conversion caused the evolution of glucocerebrosidase functional gene and pseudogene to be concerted...
  19. ncbi p53-based anti-cancer therapies: An empty promise?
    Nikolas Desilet
    Department of Biology, University of Victoria, BC, Canada
    Curr Issues Mol Biol 12:143-6. 2010
    ..However, new insights into small molecule combination therapies, microRNA regulation, structuring of clinical trials, and potential involvement in stem cell regulation may help p53 reach its potential...
  20. ncbi Novel mutations in type 2 Gaucher disease in Chinese and their functional characterization by heterologous expression
    Nelson L S Tang
    Department of Chemical Pathology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, People s Republic of China
    Hum Mutat 26:59-60. 2005
    ..Expression of novel mutations revealed that the enzyme defect could arise from one of two mechanisms: loss of catalytic activity (Y363C and M416V) or enzyme instability (P122L and N382K)...
  21. ncbi Expression of active alpha-N-acetylglucosaminidase/TAT Chimerae in cultured Spodoptera frugiperda cells
    Judith C Bandsmer
    Southern Alberta Cancer Research Institute, Dept. of Oncology, University of Calgary, Calgary, AB, Canada
    Protein Pept Lett 13:353-6. 2006
    ..All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/alpha-N-acetylglucosaminidase chimerae in enzyme replacement therapy...
  22. ncbi Generation of a conditional knockout of murine glucocerebrosidase: utility for the study of Gaucher disease
    Graham B Sinclair
    Child and Family Research Institute, Department of Medical Genetics, University of British Columbia, 950 W28th Ave Vancouver, BC, Canada V5Z 4H4
    Mol Genet Metab 90:148-56. 2007
    ..These results indicate the utility of this loxP GBA targeted murine strain for understanding the complex pathophysiology of Gaucher disease...