Xing Zhen Chen

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. pmc Structural interaction and functional regulation of polycystin-2 by filamin
    Qian Wang
    Membrane Protein Disease Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 7:e40448. 2012
  2. doi request reprint Translational up-regulation of polycystic kidney disease protein PKD2 by endoplasmic reticulum stress
    JungWoo Yang
    2Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, 7 29 Medical Sciences Bldg, Edmonton, AB T6G 2H7, Canada
    FASEB J 27:4998-5009. 2013
  3. doi request reprint Submembraneous microtubule cytoskeleton: interaction of TRPP2 with the cell cytoskeleton
    Xing Zhen Chen
    Department of Physiology, University of Alberta, Edmonton, Canada
    FEBS J 275:4675-83. 2008
  4. ncbi request reprint Alpha-actinin associates with polycystin-2 and regulates its channel activity
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7 Canada
    Hum Mol Genet 14:1587-603. 2005
  5. ncbi request reprint Troponin I binds polycystin-L and inhibits its calcium-induced channel activation
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Biochemistry 42:7618-25. 2003
  6. pmc Filamin interacts with epithelial sodium channel and inhibits its channel function
    Qian Wang
    Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 288:264-73. 2013
  7. ncbi request reprint Polycystin-2 interacts with troponin I, an angiogenesis inhibitor
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Biochemistry 42:450-7. 2003
  8. ncbi request reprint The calcium-binding EF-hand in polycystin-L is not a domain for channel activation and ensuing inactivation
    Qiang Li
    Department of Physiology, University of Alberta, 729 MSB, T6G 2H7, Edmonton, AB, Canada
    FEBS Lett 516:270-8. 2002
  9. pmc Electrophysiological characterization of a recombinant human Na+-coupled nucleoside transporter (hCNT1) produced in Xenopus oocytes
    Kyla M Smith
    Membrane Protein Research Group, Department of Physiology, Faculty of Pharmacy, 7 55 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Physiol 558:807-23. 2004
  10. ncbi request reprint Modulation of the human polycystin-L channel by voltage and divalent cations
    Yan Liu
    Membrane Protein Research Group, Department of Physiology, University of Alberta, 729 MSB, T6G 2H7, Edmonton, AB, Canada
    FEBS Lett 525:71-6. 2002

Collaborators

Detail Information

Publications28

  1. pmc Structural interaction and functional regulation of polycystin-2 by filamin
    Qian Wang
    Membrane Protein Disease Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 7:e40448. 2012
    ..Our study indicates that filamins are important regulators of polycystin-2 channel function, and further links actin cytoskeletal dynamics to the regulation of this channel protein...
  2. doi request reprint Translational up-regulation of polycystic kidney disease protein PKD2 by endoplasmic reticulum stress
    JungWoo Yang
    2Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, 7 29 Medical Sciences Bldg, Edmonton, AB T6G 2H7, Canada
    FASEB J 27:4998-5009. 2013
    ..Thus, ER stress and P-eIF2α translationally up-regulates PKD2 through bypassing the inhibitory uORF...
  3. doi request reprint Submembraneous microtubule cytoskeleton: interaction of TRPP2 with the cell cytoskeleton
    Xing Zhen Chen
    Department of Physiology, University of Alberta, Edmonton, Canada
    FEBS J 275:4675-83. 2008
    ....
  4. ncbi request reprint Alpha-actinin associates with polycystin-2 and regulates its channel activity
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7 Canada
    Hum Mol Genet 14:1587-603. 2005
    ..Thus, physical and functional interactions between PC2 and alpha-actinin may play an important role in abnormal cell adhesion, proliferation and migration observed in ADPKD...
  5. ncbi request reprint Troponin I binds polycystin-L and inhibits its calcium-induced channel activation
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Biochemistry 42:7618-25. 2003
    ..Taken together, this study suggests that troponin I acts as a regulatory subunit of the PCL channel complex and provides the first direct evidence that PCL is associated with the actin cytoskeleton through troponin I...
  6. pmc Filamin interacts with epithelial sodium channel and inhibits its channel function
    Qian Wang
    Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 288:264-73. 2013
    ..Taken together, our study demonstrated that filamin reduces ENaC channel function through direct interaction on the cell surface...
  7. ncbi request reprint Polycystin-2 interacts with troponin I, an angiogenesis inhibitor
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Biochemistry 42:450-7. 2003
    ..It is possible that altered interaction due to pathogenic polycystin-1 or -2 mutations can account for angiogenesis in ADPKD and may be corrected to some extent by exogenous troponin I...
  8. ncbi request reprint The calcium-binding EF-hand in polycystin-L is not a domain for channel activation and ensuing inactivation
    Qiang Li
    Department of Physiology, University of Alberta, 729 MSB, T6G 2H7, Edmonton, AB, Canada
    FEBS Lett 516:270-8. 2002
    ..Our data demonstrate that the EF-hand and other parts of the carboxyl tail of PCL are not determinants of channel activation/inactivation although the EF-hand seems to be involved in the modulation of these processes...
  9. pmc Electrophysiological characterization of a recombinant human Na+-coupled nucleoside transporter (hCNT1) produced in Xenopus oocytes
    Kyla M Smith
    Membrane Protein Research Group, Department of Physiology, Faculty of Pharmacy, 7 55 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Physiol 558:807-23. 2004
    ..81) and the average number of transporters present in the oocyte plasma membrane (6.8 x 10(10) per cell). The hCNT1 turnover rate at -50 mV was 9.6 molecules of uridine transported per second...
  10. ncbi request reprint Modulation of the human polycystin-L channel by voltage and divalent cations
    Yan Liu
    Membrane Protein Research Group, Department of Physiology, University of Alberta, 729 MSB, T6G 2H7, Edmonton, AB, Canada
    FEBS Lett 525:71-6. 2002
    ..This study shows that although PCL is not a voltage-gated channel, its channel activity and inhibition by Mg2+ are modulated by membrane potential...
  11. ncbi request reprint Kinesin-2 mediates physical and functional interactions between polycystin-2 and fibrocystin
    Yuliang Wu
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    Hum Mol Genet 15:3280-92. 2006
    ..Taken together, we discovered that kinesin-2 is a linker between PC2 and FPC and mediates the regulation of PC2 channel function by FPC. Our study may be important for elucidating common molecular pathways for PKD of different genotypes...
  12. pmc Receptor for activated C kinase 1 (RACK1) inhibits function of transient receptor potential (TRP)-type channel Pkd2L1 through physical interaction
    JungWoo Yang
    Department of Physiology, University of Alberta, 7 29 Medical Sciences Building, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 287:6551-61. 2012
    ..Thus, Pkd2L1 is a novel target channel whose function is regulated by the versatile scaffolding protein RACK1...
  13. ncbi request reprint A modified mammalian tandem affinity purification procedure to prepare functional polycystin-2 channel
    Qiang Li
    Department of Physiology, University of Alberta, 7 29 Medical Sciences Building, Edmonton, AB, Canada T6G 2H7
    FEBS Lett 576:231-6. 2004
    ..Thus, this modified TAP procedure provides a powerful alternative to functionally characterize membrane proteins, such as ion channels, transporters and receptors, using cell-free system derived from mammalian cells...
  14. ncbi request reprint Permeation and inhibition of polycystin-L channel by monovalent organic cations
    Xiao Qing Dai
    Membrane Protein Research Group, Department of Physiology, University of Alberta, 7 29 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Biochim Biophys Acta 1758:197-205. 2006
    ..These data allow to estimate a channel pore size of approximately 7 A for PCL...
  15. ncbi request reprint Polycystin-2 cation channel function is under the control of microtubular structures in primary cilia of renal epithelial cells
    Qiang Li
    Department of Physiology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 281:37566-75. 2006
    ..The data indicate that microtubular organization regulates PC2 function, which may explain, among others, the regulatory role of PC2 in the sensory function of primary cilia...
  16. ncbi request reprint Direct binding of alpha-actinin enhances TRPP3 channel activity
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    J Neurochem 103:2391-400. 2007
    ..It remains to be determined whether the TRPP3-alpha-actinin interaction is relevant to acid sensing and other functions in neuronal and non-neuronal cells...
  17. doi request reprint Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha
    Genqing Liang
    Membrane Protein Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Hum Mol Genet 17:3254-62. 2008
    ....
  18. doi request reprint Polycystin-2 is regulated by endoplasmic reticulum-associated degradation
    Genqing Liang
    Membrane Protein Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Hum Mol Genet 17:1109-19. 2008
    ..The ERAD machinery may thus be important for ADPKD pathogenesis because the regulation of PC2 expression by the ERAD pathway is altered by mutations in PC2...
  19. ncbi request reprint Inhibition of TRPP3 channel by amiloride and analogs
    Xiao Qing Dai
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Mol Pharmacol 72:1576-85. 2007
    ..TRPP3 may contribute to previously reported in vivo amiloride-sensitive cation transport...
  20. ncbi request reprint Inhibition of polycystin-L channel by the Chinese herb Sparganum stoloniferum Buch.-Ham
    Fang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, 729 Medical Sciences Building, Edmonton, AB T6G 2H7, Canada
    Can J Physiol Pharmacol 84:923-7. 2006
    ..Our study suggests that SBH contains 1 or more yet-to-be determined components that are inhibitors of PCL channel. The therapeutic potential of SBH for ADPKD and its chemical composition remain to be investigated...
  21. ncbi request reprint The broadly selective human Na+/nucleoside cotransporter (hCNT3) exhibits novel cation-coupled nucleoside transport characteristics
    Kyla M Smith
    Membrane Protein Research Group, Departments of Physiology and Oncology, University of Alberta Cross Cancer Institute, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 280:25436-49. 2005
    ..Chimeric studies between hCNT1 and hCNT3 located hCNT3-specific cation interactions to the C-terminal half of hCNT3, setting the stage for site-directed mutagenesis experiments to identify the residues involved...
  22. ncbi request reprint Cloning and functional characterization of the human GLUT7 isoform SLC2A7 from the small intestine
    Qiang Li
    Department of Physiology, University of Alberta, Rm 7 22 Medical Sciences Bldg, Edmonton, Alberta T6G 2H7, Canada
    Am J Physiol Gastrointest Liver Physiol 287:G236-42. 2004
    ..Comparison between these proteins may provide key information as to the structural determinants for the recognition of fructose as a substrate...
  23. ncbi request reprint Polycystin-2 associates with tropomyosin-1, an actin microfilament component
    Qiang Li
    Membrane Protein Research Group, Department of Physiology, University of Alberta, 7 29 Medical Sciences Building, T6G 2H7, Edmonton, AB, Canada
    J Mol Biol 325:949-62. 2003
    ....
  24. ncbi request reprint Detecting protein-protein interactions by Far western blotting
    Yuliang Wu
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Nat Protoc 2:3278-84. 2007
    ..Furthermore, in cases where they bind to each other indirectly, Far WB allows the examination of candidate protein(s) that form a complex between them. Typically, 2-3 d are required to carry out the experiment...
  25. ncbi request reprint Effect of hydro-osmotic pressure on polycystin-2 channel function in the human syncytiotrophoblast
    Nicolás Montalbetti
    Laboratorio de Canales Iónicos, Departamento de Fisicoquimica y Quimica Analitica, Facultad de Farmacia y Bioquimica, Buenos Aires, Argentina
    Pflugers Arch 451:294-303. 2005
    ..This co-localization was disrupted by cytochalasin D. Taken together, our findings indicate that physical forces exerted on cells regulate PC2 channel activity by a sensory mechanism involving the actin cytoskeleton...
  26. pmc Polycystin-2 cation channel function in the human syncytiotrophoblast is regulated by microtubular structures
    Nicolás Montalbetti
    Laboratorio de Canales Iónicos, Departamento de Fisicoquimica y Quimica Analitica, Facultad de Farmacia y Bioquimica, Buenos Aires, Argentina
    J Physiol 579:717-28. 2007
    ..We believe that the data are the first to provide a direct demonstration of a microtubular interaction with PC2 in the hST. This interaction thus plays an important regulatory role in the control of ion transport in the human placenta...
  27. pmc Cytoskeletal regulation of calcium-permeable cation channels in the human syncytiotrophoblast: role of gelsolin
    Nicolás Montalbetti
    Laboratorio de Canales Iónicos, Departmento de Fisicoquímica y Química Analítica, Facultad de Farmica y Bioquímica, Buenos Aires, Argentina
    J Physiol 566:309-25. 2005
    ..Cytoskeletal structures may thus be relevant to ion transport regulation in the human placenta...
  28. ncbi request reprint More than colocalizing with polycystin-1, polycystin-L is in the centrosome
    Eva Flore Bui-Xuan
    Département de Physiologie et Biophysique, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    Am J Physiol Renal Physiol 291:F395-406. 2006
    ..Interestingly, we also identified a novel site for a polycystin member (polycystin-L) in unciliated cells, the centrosome, which allowed us to reveal an involvement of polycystin-l in cell proliferation...