John P Chang

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. ncbi Nitric oxide produced by a novel nitric oxide synthase isoform is necessary for gonadotropin-releasing hormone-induced growth hormone secretion via a cGMP-dependent mechanism
    A D Uretsky
    Departments of Biological Sciences and Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 15:667-76. 2003
  2. ncbi Kisspeptin-1 directly stimulates LH and GH secretion from goldfish pituitary cells in a Ca(2+)-dependent manner
    John P Chang
    Department of Biological Sciences, University of Alberta, Canada T6G 2E9
    Gen Comp Endocrinol 179:38-46. 2012
  3. ncbi Calcium and other signalling pathways in neuroendocrine regulation of somatotroph functions
    John P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Cell Calcium 51:240-52. 2012
  4. ncbi Signal transduction mechanisms mediating secretion in goldfish gonadotropes and somatotropes
    J P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Biochem Cell Biol 78:139-53. 2000
  5. ncbi Role of Ca2+ stores in dopamine- and PACAP-evoked growth hormone release in goldfish
    J P Chang
    Department of Biological Sciences, University of Alberta, CW 405, Biological Sciences Building, Edmonton, Alta, Canada T6G 2E9
    Mol Cell Endocrinol 206:63-74. 2003
  6. ncbi Pacap stimulation of gonadotropin-II secretion in goldfish pituitary cells: mechanisms of action and interaction with gonadotropin releasing hormone signalling
    J P Chang
    Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
    J Neuroendocrinol 13:540-50. 2001
  7. ncbi Signal transduction in multifactorial neuroendocrine control of gonadotropin secretion and synthesis in teleosts-studies on the goldfish model
    John P Chang
    Department of Biological Sciences, University of Alberta, CW405 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9
    Gen Comp Endocrinol 161:42-52. 2009
  8. ncbi PACAP stimulation of maturational gonadotropin secretion in goldfish involves extracellular signal-regulated kinase, but not nitric oxide or guanylate cyclase, signaling
    John P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Gen Comp Endocrinol 165:127-35. 2010
  9. ncbi Extracellular signal-regulated kinase mediates gonadotropin subunit gene expression and LH release responses to endogenous gonadotropin-releasing hormones in goldfish
    Christian Klausen
    Department of Biological Sciences, University of Calgary, 2500 University Drive, N W, Calgary, Alta, Canada T2N 1N4
    Gen Comp Endocrinol 158:36-46. 2008
  10. ncbi Endogenous hypothalamic somatostatins differentially regulate growth hormone secretion from goldfish pituitary somatotropes in vitro
    Warren K Yunker
    Department of Biological Sciences, Faculty of Science, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
    Endocrinology 144:4031-41. 2003

Collaborators

Detail Information

Publications50

  1. ncbi Nitric oxide produced by a novel nitric oxide synthase isoform is necessary for gonadotropin-releasing hormone-induced growth hormone secretion via a cGMP-dependent mechanism
    A D Uretsky
    Departments of Biological Sciences and Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 15:667-76. 2003
    ..Furthermore, these data suggest that NO, most likely through the generation of cGMP, is a necessary signal transduction component of GnRH-induced GH secretion...
  2. ncbi Kisspeptin-1 directly stimulates LH and GH secretion from goldfish pituitary cells in a Ca(2+)-dependent manner
    John P Chang
    Department of Biological Sciences, University of Alberta, Canada T6G 2E9
    Gen Comp Endocrinol 179:38-46. 2012
    ..These results indicate that, in goldfish, (1) direct gKiss1 actions on pituitary LH and GH secretion exist, (2) these actions are independent of GnRH and (3) they involve Ca(2+) signalling...
  3. ncbi Calcium and other signalling pathways in neuroendocrine regulation of somatotroph functions
    John P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Cell Calcium 51:240-52. 2012
    ....
  4. ncbi Signal transduction mechanisms mediating secretion in goldfish gonadotropes and somatotropes
    J P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Biochem Cell Biol 78:139-53. 2000
    ..The interaction of different second messenger systems provide the basis by which regulation of maturational gonadotropin and somatotropin release by multiple neuroendocrine factors can be integrated at the target cell level...
  5. ncbi Role of Ca2+ stores in dopamine- and PACAP-evoked growth hormone release in goldfish
    J P Chang
    Department of Biological Sciences, University of Alberta, CW 405, Biological Sciences Building, Edmonton, Alta, Canada T6G 2E9
    Mol Cell Endocrinol 206:63-74. 2003
    ..As GH secretion stimulated by two endogenous gonadotropin-releasing hormones is not affected by Tg, it appears that distinct multiple Ca2+ stores mediate the hormone releasing response to different neuroendocrine regulators...
  6. ncbi Pacap stimulation of gonadotropin-II secretion in goldfish pituitary cells: mechanisms of action and interaction with gonadotropin releasing hormone signalling
    J P Chang
    Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
    J Neuroendocrinol 13:540-50. 2001
    ....
  7. ncbi Signal transduction in multifactorial neuroendocrine control of gonadotropin secretion and synthesis in teleosts-studies on the goldfish model
    John P Chang
    Department of Biological Sciences, University of Alberta, CW405 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9
    Gen Comp Endocrinol 161:42-52. 2009
    ....
  8. ncbi PACAP stimulation of maturational gonadotropin secretion in goldfish involves extracellular signal-regulated kinase, but not nitric oxide or guanylate cyclase, signaling
    John P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Gen Comp Endocrinol 165:127-35. 2010
    ..These results suggest that although increased availability of NO and cGMP can lead to increased GTH-II secretion, MEK/ERK signaling, rather than NOS/NO/GC activation, mediates PACAP action on GTH-II release in goldfish...
  9. ncbi Extracellular signal-regulated kinase mediates gonadotropin subunit gene expression and LH release responses to endogenous gonadotropin-releasing hormones in goldfish
    Christian Klausen
    Department of Biological Sciences, University of Calgary, 2500 University Drive, N W, Calgary, Alta, Canada T2N 1N4
    Gen Comp Endocrinol 158:36-46. 2008
    ..More importantly, the present study suggests that GnRH-induced gonadotropin subunit gene expression and LH release involve MEK/ERK signaling in goldfish...
  10. ncbi Endogenous hypothalamic somatostatins differentially regulate growth hormone secretion from goldfish pituitary somatotropes in vitro
    Warren K Yunker
    Department of Biological Sciences, Faculty of Science, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
    Endocrinology 144:4031-41. 2003
    ..This raises the possibility that such differences may allow for the selective regulation of various aspects of somatotrope function by different SS peptides...
  11. ncbi Differential involvement of nitric oxide signaling in dopamine and PACAP stimulation of growth hormone release in goldfish
    Gabriel Mitchell
    Department of Biological Sciences, University of Alberta, CW 405, Biological Sciences Building, Edmonton, Alta, Canada T6G 2E9
    Gen Comp Endocrinol 155:318-27. 2008
    ..These results indicate that although both PACAP and DA stimulation of acute GH release involve activation of adenylate cyclase/cAMP, DA- but not PACAP-signaling also utilizes the NOS/NO second messenger system...
  12. ncbi Role of PKC in the regulation of gonadotropin subunit mRNA levels: interaction with two native forms of gonadotropin-releasing hormone
    Christian Klausen
    Dept. of Biological Sciences, Univ. of Calgary, Calgary, Alberta, Canada, T2N 1N4
    Am J Physiol Regul Integr Comp Physiol 289:R1634-43. 2005
    ..Our data illustrate major differences in the signal transduction of GnRH effects on GtH secretion and gene expression in the goldfish pituitary...
  13. ncbi Differential involvement of phosphoinositide 3-kinase in gonadotrophin-releasing hormone actions in gonadotrophs and somatotrophs of goldfish, Carassius auratus
    Joshua G Pemberton
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 23:660-74. 2011
    ..In gonadotrophs, the inhibitory action of PI3K on both sGnRH and cGnRH-II involves the attenuation of their evoked [Ca(2+)](i); in contrast, GnRH isoform-specific effects occur in somatotrophs...
  14. ncbi PKC and ERK are differentially involved in gonadotropin-releasing hormone-induced growth hormone gene expression in the goldfish pituitary
    Christian Klausen
    Dept. of Biological Sciences, Univ. of Calgary, Calgary, Alberta, Canada, T2N 1N4
    Am J Physiol Regul Integr Comp Physiol 289:R1625-33. 2005
    ..The results presented here provide novel insights into the functional specificity of GnRH-induced signaling and the regulation of GH gene expression...
  15. ncbi Ghrelin-induced growth hormone release from goldfish pituitary cells is nitric oxide dependent
    Caleb L Grey
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9
    Gen Comp Endocrinol 179:152-8. 2012
    ....
  16. ncbi Function-specific calcium stores selectively regulate growth hormone secretion, storage, and mRNA level
    James D Johnson
    Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9, Canada T2N 1N4
    Am J Physiol Endocrinol Metab 282:E810-9. 2002
    ..Collectively, these results suggest that parts of the secretory pathway can be controlled independently by function-specific Ca(2+) stores...
  17. ncbi Ghrelin stimulation of gonadotropin (LH) release from goldfish pituitary cells: presence of the growth hormone secretagogue receptor (GHS-R1a) and involvement of voltage-sensitive Ca2+ channels
    Caleb L Grey
    Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada
    Mol Cell Endocrinol 317:64-77. 2010
    ..These results indicate that GHS-R1a is present in goldfish pituitary and Ca(2+) entry through VSCC mediates direct gGRLN(19) action on LH release in goldfish pituitary cells...
  18. ncbi Caffeine-stimulated GTH-II release involves Ca(2+) stores with novel properties
    James D Johnson
    Department of Biological Sciences, Biological Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
    Am J Physiol Cell Physiol 282:C635-45. 2002
    ..Collectively, these data indicate that caffeine-stimulated GTH-II release is not mediated by typical agonist-sensitive Ca(2+) stores found in endoplasmic reticulum...
  19. ncbi Serotonin interferes with Ca2+ and PKC signaling to reduce gonadotropin-releasing hormone-stimulated GH secretion in goldfish pituitary cells
    Yi Yu
    Department of Biological Sciences, CW 405, Biological Science Building, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
    Gen Comp Endocrinol 159:58-66. 2008
    ..The differential Ca(2+) responses by depolarizing doses of K(+) is consistent with our previous findings that sGnRH and cGnRH-II are coupled to overlapping and yet distinct Ca(2+)-dependent mechanisms...
  20. ncbi Goldfish brain somatostatin-28 differentially affects dopamine- and pituitary adenylate cyclase-activating polypeptide-induced GH release and Ca(2+) and cAMP signals
    Yi Yu
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Mol Cell Endocrinol 332:283-92. 2011
    ..However, DA D1 receptor activation bypassed gbSS-28 inhibitory effects on cAMP production and NO actions via unknown mechanisms to maintain a normal [Ca(2+)](i) response leading to unhampered GH release...
  21. ncbi Calcium buffering activity of mitochondria controls basal growth hormone secretion and modulates specific neuropeptide signaling
    James D Johnson
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Cell Calcium 37:573-81. 2005
    ..Thus, we provide the first evidence that mitochondrial Ca(2+) buffering is differentially involved in specific agonist Ca(2+) signaling pathways and plays an important role in the control of basal GH release...
  22. ncbi Time- and dose-related effects of gonadotropin-releasing hormone on growth hormone and gonadotropin subunit gene expression in the goldfish pituitary
    Christian Klausen
    Department of Biological Sciences, University of Calgary, AB, Canada
    Can J Physiol Pharmacol 80:915-24. 2002
    ....
  23. ncbi Somatostatin-14 actions on dopamine- and pituitary adenylate cyclase-activating polypeptide-evoked Ca2+ signals and growth hormone secretion
    W K Yunker
    Department of Biological Sciences, Faculty of Science, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 16:684-94. 2004
    ..Furthermore, it appears likely that the cellular mechanisms underlying the observed effects of SS(14) on Ca(2+) signalling are upstream of cAMP and may be unrelated to those responsible for inhibiting GH release...
  24. ncbi Signal transduction pathways in GnRH- and dopamine D1-stimulated growth hormone secretion in the goldfish
    J P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Chin J Physiol 37:111-27. 1994
    ..These signalling mechanisms appear to act on distinct GH pools. PKC and cAMP subsequently activate VSCC. Ca2+ entry through VSCC plays a role in the sustained GH release response by enhancing the PKC- and cAMP-induced GH release...
  25. ncbi Ghrelin-induced growth hormone release from goldfish pituitary cells involves voltage-sensitive calcium channels
    Caleb L Grey
    Department of Biological Sciences, University of Alberta, Edmonton, Alta, Canada T6G 2E9
    Gen Comp Endocrinol 160:148-57. 2009
    ..Taken together, the results of this study provide evidence that entry of extracellular Ca(2+) through LVSCCs is a key component of the GRL signalling pathway leading to GH release in the goldfish pituitary...
  26. ncbi Caffeine stores and dopamine differentially require Ca(2+) channels in goldfish somatotropes
    C J Wong
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    Am J Physiol Regul Integr Comp Physiol 280:R494-503. 2001
    ..This differential temporal requirement for Ca(2+) channels in Ca(2+) signaling may be a mechanism to segregate intracellular signaling pathways of multiple neuroendocrine regulators in the teleost pituitary...
  27. ncbi A gonadotropin-releasing hormone insensitive, thapsigargin-sensitive Ca2+ store reduces basal gonadotropin exocytosis and gene expression: comparison with agonist-sensitive Ca2+ stores
    J D Johnson
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 15:204-14. 2003
    ..Our data show that GTH-II secretion, storage and transcription can be independently controlled by distinct Ca2+ stores...
  28. ncbi Intracellular calcium involvement in pituitary adenylate cyclase-activating polypeptide stimulation of growth hormone and gonadotrophin secretion in goldfish pituitary cells
    G R Sawisky
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    J Neuroendocrinol 17:353-71. 2005
    ..PC-PLC and some SERCA negatively modulate PACAP actions but mitochondrial Ca(2+) stores per se are not important. A novel PI-PLC mechanism, which does not involve the traditional IP(3)/Ca(2+) pathway, is also suggested...
  29. ncbi Agonist-specific and sexual stage-dependent inhibition of gonadotropin-releasing hormone-stimulated gonadotropin and growth hormone release by ryanodine: relationship to sexual stage-dependent caffeine-sensitive hormone release
    J D Johnson
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 14:144-55. 2002
    ..Ours study is the first to suggest that RyR2 may be involved in the seasonal plasticity of pituitary function, which may be related to cyclic changes observed in reproduction and growth...
  30. ncbi Characterization of ionic currents and electrophysiological properties of goldfish somatotropes in primary culture
    Yi Yu
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Gen Comp Endocrinol 169:231-43. 2010
    ..Opening of voltage-sensitive Ca(2+) channels during AP firing leads to increases in [Ca(2+)]i...
  31. ncbi Modulation of gonadotropin II release by K+ channel blockers in goldfish gonadotropes: a novel stimulatory action of 4-aminopyridine
    C J Wong
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Neuroendocrinol 13:951-8. 2001
    ....
  32. ncbi Somatostatin actions on a protein kinase C-dependent growth hormone secretagogue cascade
    W K Yunker
    Department of Biological Sciences, CW 405 Biological Sciences Building, Faculty of Science, University of Alberta, Edmonton, T6G 2E9, Alberta, Canada
    Mol Cell Endocrinol 175:193-204. 2001
    ..These data establish that abolition of Ca2+ signals is not a primary mechanism through which SS lowers basal, or inhibits GnRH-stimulated hormone release...
  33. ncbi PKC mediates GnRH activation of a Na+/H+ exchanger in goldfish somatotropes
    Guang Li Li
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Gen Comp Endocrinol 166:296-306. 2010
    ..Since amiloride and Na(+)-free solution caused acidification in somatotropes at rest, regulation of basal pH(i) in these cells likely involves Na(+) flux through amiloride-sensitive NHE...
  34. ncbi Differences in the acute actions of sGnRH and cGnRH-II on gonadotropin release in goldfish pituitary cells
    J P Chang
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Gen Comp Endocrinol 100:339-54. 1995
    ..Taken together, these results strongly indicate that the mechanisms mediating rapid sGnRH-induced and cGnRH-II-elicited GTH responses are different as in the case for prolonged GnRH action...
  35. ncbi Evidence that nitric oxide is involved in the regulation of growth hormone secretion in goldfish
    A D Uretsky
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Gen Comp Endocrinol 118:461-70. 2000
    ..AGH and 1400W did not alter basal GH secretion. These data suggest that NO plays a role in mediating the GH response to endogenous neuroendocrine factors in goldfish...
  36. ncbi Neuroendocrine control of growth hormone in fish
    Luis Fabian Canosa
    Department of Biological Sciences, University of Alberta, Edmonton, Alta, Canada T6G 2E9
    Gen Comp Endocrinol 151:1-26. 2007
    ..However, more work remains to be done in order to better understand the integrative mechanisms of signal transduction at the somatotrope level and the relevance of various GH regulators in different physiological circumstances...
  37. ncbi Differential actions of dopamine receptor subtypes on gonadotropin and growth hormone release in vitro in goldfish
    J P Chang
    Department of Zoology, University of Alberta, Edmonton, Canada
    Neuroendocrinology 51:664-74. 1990
    ..abstract truncated at 400 words)..
  38. ncbi The effect of gonadotropin-releasing hormone on growth hormone and gonadotropin subunit gene expression in the pituitary of goldfish, Carassius auratus
    C Klausen
    Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, Calgary, T2N 1N4, Alberta, Canada
    Comp Biochem Physiol B Biochem Mol Biol 129:511-6. 2001
    ..The present results provide a basis for the investigation of the signal transduction pathways that mediate GnRH-induced changes in GtH subunit and GH mRNA levels in the goldfish pituitary...
  39. ncbi Evidence that gonadotropin-releasing hormone also functions as a growth hormone-releasing factor in the goldfish
    T A Marchant
    Department of Zoology, University of Alberta, Edmonton, Canada
    Endocrinology 124:2509-18. 1989
    ....
  40. ncbi Participation of arachidonic acid metabolism in gonadotropin-releasing hormone stimulation of goldfish gonadotropin release
    J P Chang
    Department of Zoology, University of Alberta, Edmonton, Canada
    Gen Comp Endocrinol 76:2-11. 1989
    ..These results provide in vivo and in vitro evidence for the participation of AA metabolism in mediating GnRH-stimulated GTH release in the goldfish. The importance of AA metabolism through the lipoxygenase pathway is also indicated...
  41. ncbi Involvement of protein kinase C and intracellular Ca2+ in goldfish brain somatostatin-28 inhibitory action on growth hormone release in goldfish
    Y Yu
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Gen Comp Endocrinol 168:71-81. 2010
    ..Our results suggest that gbSS-28 inhibits GnRH-induced GH release in part by attenuating PKC-mediated GnRH [Ca(2+)](i) signals. gbSS-28 reduces basal GH release also via reduction in [Ca(2+)](i) but PKC is not involved in this regard...
  42. ncbi Multiplicity of gonadotropin-releasing hormone signaling: a comparative perspective
    Christian Klausen
    Department of Biological Sciences, University of Calgary, 2500 University Drive N.W, Calgary, AB T2N 1N4, Canada
    Prog Brain Res 141:111-28. 2002
    ....
  43. ncbi Influences of norepinephrine, and adrenergic agonists and antagonists on gonadotropin secretion from dispersed pituitary cells of goldfish, Carassius auratus
    J P Chang
    Department of Zoology, University of Alberta, Edmonton, Canada
    Neuroendocrinology 54:202-10. 1991
    ..These results suggests that NE directly stimulates GTH secretion independent of sGnRH receptors via alpha 1-like adrenergic receptors...
  44. ncbi Growth hormone-releasing hormone stimulates GH release while inhibiting ghrelin- and sGnRH-induced LH release from goldfish pituitary cells
    Caleb L Grey
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9
    Gen Comp Endocrinol 186:150-6. 2013
    ....
  45. ncbi Nitric oxide signaling in ghrelin-induced LH release from goldfish pituitary cells
    Caleb L Grey
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada
    Gen Comp Endocrinol 183:7-13. 2013
    ....
  46. ncbi Purification and isolation of corticosteroidogenic cells from head kidney of rainbow trout (Oncorhynchus mykiss) for testing cell-specific effects of a pesticide
    Alice Hontela
    Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada T1K 3M4
    Comp Biochem Physiol C Toxicol Pharmacol 147:52-60. 2008
    ..The Percoll method for isolation of different cell types from the head kidney facilitated a study of cell-specific effects of a pesticide...
  47. ncbi Non-denaturing assay for the determination of the potency of recombinant bovine somatotropin by high-performance size-exclusion chromatography
    J P Chang
    Lilly Research Laboratories, Eli Lilly and Co, Greenfield, IN 46140
    J Chromatogr A 675:113-22. 1994
    ..The bio-inactive aggregates can be separated by the method developed in this study. The high-low chromatographic technique has been used to estimate rbST soluble aggregates in bulk materials...
  48. ncbi The effects of GABA agonists on glutamic acid decarboxylase, GABA-transaminase, activin, salmon gonadotrophin-releasing hormone and tyrosine hydroxylase mRNA in the goldfish (Carassius auratus) neuroendocrine brain
    C J Martyniuk
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    J Neuroendocrinol 19:390-6. 2007
    ....
  49. ncbi Pituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish
    A O Wong
    Department of Zoology, University of Hong Kong
    Biochem Cell Biol 78:329-43. 2000
    ....
  50. ncbi Improved potency assay for recombinant bovine somatotropin by high-performance size-exclusion chromatography
    J P Chang
    Lilly Research Laboratories, Eli Lilly and Co, Greenfield, IN 46140, USA
    J Chromatogr A 736:97-104. 1996
    ..The rbST monomer and dimer in the separation were verified by liquid chromatography-electrospray mass spectrometry. This method was optimized and validated...