Hong Chang

Summary

Affiliation: University of Toronto
Country: Canada

Publications

  1. doi request reprint PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa
    Manujendra N Saha
    Corresponding Author Hong Chang, Toronto General Hospital, University Health Network, 200 Elizabeth Street, 11E 413, Toronto, ON M5G 2C4, Canada
    Mol Cancer Ther 12:2331-41. 2013
  2. pmc Targeting p53 by small molecules in hematological malignancies
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Research Institute, Toronto, Canada
    J Hematol Oncol 6:23. 2013
  3. pmc Suppressing miRNA-15a/-16 expression by interleukin-6 enhances drug-resistance in myeloma cells
    Mu Hao
    State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Science and Peking Union Medical College Tianjin China
    J Hematol Oncol 4:37. 2011
  4. pmc Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma
    Johann Micallef
    Department of Laboratory Hematology, University Health Network, Toronto, Canada
    J Hematol Oncol 3:13. 2010
  5. ncbi request reprint Prognostic relevance of CD56 expression in multiple myeloma: a study including 107 cases treated with high-dose melphalan-based chemotherapy and autologous stem cell transplant
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, ON, Canada
    Leuk Lymphoma 47:43-7. 2006
  6. ncbi request reprint Genetic risk identifies multiple myeloma patients who do not benefit from autologous stem cell transplantation
    H Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Ontario, Canada
    Bone Marrow Transplant 36:793-6. 2005
  7. ncbi request reprint Analysis of PTEN deletions and mutations in multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Ont, Canada M5G 2M9
    Leuk Res 30:262-5. 2006
  8. ncbi request reprint The absence of CD56 on malignant plasma cells in the cerebrospinal fluid is the hallmark of multiple myeloma involving central nervous system
    Hong Chang
    Department of Laboratory Haematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    Br J Haematol 129:539-41. 2005
  9. ncbi request reprint Identification of cell lineages involved by t(15;17) in acute promyelocytic leukemia by combined fluorescence activated cell sorting and FISH
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 158:43-8. 2005
  10. ncbi request reprint Immunohistochemistry accurately predicts FGFR3 aberrant expression and t(4;14) in multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, McLaughlin Center for Molecular Medicine, University of Toronto, ON, Canada
    Blood 106:353-5. 2005

Collaborators

Detail Information

Publications92

  1. doi request reprint PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa
    Manujendra N Saha
    Corresponding Author Hong Chang, Toronto General Hospital, University Health Network, 200 Elizabeth Street, 11E 413, Toronto, ON M5G 2C4, Canada
    Mol Cancer Ther 12:2331-41. 2013
    ..Our study for the first time shows antimyeloma activity of PRIMA-1(Met) and provides the rationale for its clinical evaluation in patients with multiple myeloma, including the high-risk group with p53 mutation/deletion...
  2. pmc Targeting p53 by small molecules in hematological malignancies
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Research Institute, Toronto, Canada
    J Hematol Oncol 6:23. 2013
    ..In addition, we discuss how p53 tumor suppressor protein holds promise as a drug target for recent and future novel therapies in these diseases...
  3. pmc Suppressing miRNA-15a/-16 expression by interleukin-6 enhances drug-resistance in myeloma cells
    Mu Hao
    State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Science and Peking Union Medical College Tianjin China
    J Hematol Oncol 4:37. 2011
    ..In conclusion, our data suggest that via suppressing miRNA-15a and -16 expressions, IL-6 secreted by BMSCs promotes drug-resistance in myeloma cells...
  4. pmc Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma
    Johann Micallef
    Department of Laboratory Hematology, University Health Network, Toronto, Canada
    J Hematol Oncol 3:13. 2010
    ..In addition, mass spectrometry was employed to study relevant signaling pathways activated in MM. This review will focus on how mass spectrometry has been applied to increase our understanding of MM...
  5. ncbi request reprint Prognostic relevance of CD56 expression in multiple myeloma: a study including 107 cases treated with high-dose melphalan-based chemotherapy and autologous stem cell transplant
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, ON, Canada
    Leuk Lymphoma 47:43-7. 2006
    ....
  6. ncbi request reprint Genetic risk identifies multiple myeloma patients who do not benefit from autologous stem cell transplantation
    H Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Ontario, Canada
    Bone Marrow Transplant 36:793-6. 2005
    ..High-risk patients do not benefit from stem cell transplant and should be offered alternative therapies...
  7. ncbi request reprint Analysis of PTEN deletions and mutations in multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Ont, Canada M5G 2M9
    Leuk Res 30:262-5. 2006
    ..Our results indicate that alterations of PTEN are uncommon in MM patients, and PTEN deletions tend to occur in advanced disease suggesting that they are secondary, rather than primary, events in the pathogenesis of MM...
  8. ncbi request reprint The absence of CD56 on malignant plasma cells in the cerebrospinal fluid is the hallmark of multiple myeloma involving central nervous system
    Hong Chang
    Department of Laboratory Haematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    Br J Haematol 129:539-41. 2005
    ..0001). The prevalence of CD56-negative myeloma cells in the BM and CSF of our patients suggests that CD56 downregulation may play a role in the pathogenesis of CNS MM...
  9. ncbi request reprint Identification of cell lineages involved by t(15;17) in acute promyelocytic leukemia by combined fluorescence activated cell sorting and FISH
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 158:43-8. 2005
    ..These results suggest that lymphocytes and erythroblasts are not clonally involved in APL, and that malignant transformation in some cases of APL may occur at the level of CD34+ cells...
  10. ncbi request reprint Immunohistochemistry accurately predicts FGFR3 aberrant expression and t(4;14) in multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, McLaughlin Center for Molecular Medicine, University of Toronto, ON, Canada
    Blood 106:353-5. 2005
    ..001), a shorter progression-free survival (median, 11.5 versus 25.8 months; P < .001), and a shorter overall survival (median, 19.2 versus 46.3 months; P < .001)...
  11. pmc CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, University Health Network, 200 Elizabeth Street, 11E 413 Toronto, ON, M5G 2C4
    Haematologica 95:1542-7. 2010
    ..The aim of this study was to correlate nuclear CKS1B protein immunoreactivity, 1q21 amplification status, p27(Kip1) expression and survival in patients with newly-diagnosed multiple myeloma...
  12. ncbi request reprint Clonality analysis of cell lineages in acute myeloid leukemia with inversion 16
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, 610 University Avenue, 4 320 Toronto, ON, M5G 2M9, Canada
    Cancer Genet Cytogenet 156:175-8. 2005
    ..The current study provides a direct evidence for the clonal involvement of myeloid lineage cells and B-lymphocytes and suggests that T-cells are not part of the malignant clone in this disease...
  13. ncbi request reprint Analysis of 6q deletion in Waldenstrom macroglobulinemia
    Hong Chang
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada
    Eur J Haematol 79:244-7. 2007
    ..92). Our study confirms that 6q deletion is a frequent event, but it does not appear to be a prognostic marker for patients with WM...
  14. ncbi request reprint CD7 expression predicts poor disease free survival and post-remission survival in patients with acute myeloid leukemia and normal karyotype
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ont, Canada M5G 2M9
    Leuk Res 31:157-62. 2007
    ..003 and P=0.0157, respectively) and shorter OS (P</=0.001 and P=0.007, respectively). Multivariate analysis of age, WBC, CD34, HLA-DR and CD7 showed that CD7 expression was an independent risk factor for DFS (P=0.01) and PRS (P=0.02)...
  15. ncbi request reprint Aberrant nuclear p53 protein expression detected by immunohistochemistry is associated with hemizygous P53 deletion and poor survival for multiple myeloma
    Hong Chang
    Laboratory Hematology, and Laboratory Medicine and Pathobiology, University Health Network, University of Toronto, Toronto, ON, Canada
    Br J Haematol 138:324-9. 2007
    ..Our data indicate that nuclear p53 protein expression, detected by a widely available immunohistochemical method, is strongly associated with TP53 deletion and an adverse clinical outcome for MM...
  16. doi request reprint Genetic aberrations including chromosome 1 abnormalities and clinical features of plasma cell leukemia
    Hong Chang
    Department of Laboratory Hematology, University Health Network, Toronto, Canada
    Leuk Res 33:259-62. 2009
    ..008)...
  17. ncbi request reprint Chromosome 1p21 deletion is a novel prognostic marker in patients with multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada
    Br J Haematol 139:51-4. 2007
    ..002) than those without 1p21 deletions. On multivariate analysis, which included deletions of 13q, TP53, t(4;14) and CKS1B amplification, 1p21 deletion remained as an independent risk factor for PFS (P = 0.01) and OS (P = 0.04)...
  18. ncbi request reprint Bortezomib therapy response is independent of cytogenetic abnormalities in relapsed/refractory multiple myeloma
    Hong Chang
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, Canada
    Leuk Res 31:779-82. 2007
    ..Our data suggest that, in this pilot study, bortezomib is an effective salvage therapy for refractory/relapsed myeloma, irrespective of genetic risk factors...
  19. ncbi request reprint Multiple myeloma patients with CKS1B gene amplification have a shorter progression-free survival post-autologous stem cell transplantation
    Hong Chang
    Department of Laboratory Hematology, Toronto General Hospital, University Health Network, Toronto, Canada
    Br J Haematol 135:486-91. 2006
    ..In this cohort of patients, CKS1B was frequently amplified in MM and may represent genetic instability associated with disease progression...
  20. doi request reprint Prognostic relevance of 6q deletion in Waldenström's macroglobulinemia: a multicenter study
    Hong Chang
    University Health Network, Toronto, Canada
    Clin Lymphoma Myeloma 9:36-8. 2009
    ..46), or overall survivals in patients with and without del (6q) (163 months vs. not reached; P = .83). Our study confirms that the 6q deletion is a frequent event, but it does not appear to affect the clinical outcome of WM...
  21. ncbi request reprint Significant increase of CKS1B amplification from monoclonal gammopathy of undetermined significance to multiple myeloma and plasma cell leukaemia as demonstrated by interphase fluorescence in situ hybridisation
    Hong Chang
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada
    Br J Haematol 134:613-5. 2006
    ..001). Our results suggest that CKS1B amplification is associated with transformation from MGUS to MM and progression to PCL...
  22. doi request reprint 1p21 deletions are strongly associated with 1q21 gains and are an independent adverse prognostic factor for the outcome of high-dose chemotherapy in patients with multiple myeloma
    H Chang
    Department of Laboratory Hematology, University Health Network, Toronto, Ontario, Canada
    Bone Marrow Transplant 45:117-21. 2010
    ..003) and OS (P=0.013) after adjusting for del(13q), del(p53), t(4;14) and 1q21 amplifications. Our results indicate that del(1p21) is an independent poor prognostic factor associated with disease progression in MM...
  23. ncbi request reprint Genomic aberrations in plasma cell leukemia shown by interphase fluorescence in situ hybridization
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, Department of Laboratory Medicine and Pathobiology, 610 University Avenue, University of Toronto, Toronto ON M5G 2M9, Canada
    Cancer Genet Cytogenet 156:150-3. 2005
    ..The frequent TP53 deletions may represent a marker of genetic instability giving rise to an increased propensity for myeloma cells to emigrate from the bone marrow environment and enter leukemic phase...
  24. doi request reprint Impact of cytogenetics in patients with relapsed or refractory multiple myeloma treated with bortezomib: Adverse effect of 1q21 gains
    Hong Chang
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, Canada
    Leuk Res 35:95-8. 2011
    ..Multivariate analysis confirmed that 1q21 gain is an independent risk factor for PFS (p=0.03) and OS (p=0.009) of bortezomib-treated relapsed/refractory myeloma...
  25. ncbi request reprint Chronic lymphocytic leukemia in the course of chronic myelocytic leukemia: evidence of independent clonal origin as shown by interphase fluorescence in situ hybridization and fluorescence-activated cell sorting
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, 610 University Ave, Toronto, ON M5G 2M9, Canada
    Cancer Genet Cytogenet 152:146-8. 2004
    ..We provide evidence that the CLL cells arose in a Ph(-) clone...
  26. ncbi request reprint Molecular cytogenetic abnormalities in patients with concurrent chronic lymphocytic leukemia and multiple myeloma shown by interphase fluorescence in situ hybridization: evidence of distinct clonal origin
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital and University Health Network, University of Toronto, 610 University Avenue, 4 320, Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 148:44-8. 2004
    ..Our results show that the cytogenetic profiles differ between CLL and MM within the same patients, and provide evidence for two distinct malignant clones in both patients...
  27. ncbi request reprint Interphase cytogenetic analysis of clonality in peripheral blood cells from a patient with Down syndrome and acute megakaryoblastic leukemia
    Hong Chang
    Princess Margaret Hospital, University Health Network, University of Toronto, 4 320 Toronto, Ontario M5G 2M9, Canada
    Cancer Genet Cytogenet 148:141-4. 2004
    ..The transformation occurs in progenitor cells with limited myeloid differentiation and without involvement of lymphoid lineage cells...
  28. ncbi request reprint Extramedullary infiltrates of AML are associated with CD56 expression, 11q23 abnormalities and inferior clinical outcome
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, Toronto, ON, Canada
    Leuk Res 28:1007-11. 2004
    ..0016). Our study of adult AML patients demonstrates that EMI at diagnosis is associated with CD56 expression by leukemic blasts, 11q23 karyotypic abnormalities, low complete remission rate and poor overall survival...
  29. ncbi request reprint Prognostic relevance of immunophenotyping in 379 patients with acute myeloid leukemia
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Ont, Canada M5G 2M9
    Leuk Res 28:43-8. 2004
    ..Defined by number of factors, this score system may help to stratify AML patients to alternative treatment for better outcome...
  30. ncbi request reprint The t(4;14) is associated with poor prognosis in myeloma patients undergoing autologous stem cell transplant
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, Canada
    Br J Haematol 125:64-8. 2004
    ..Our results indicate that the t(4;14) detected by cIg-FISH is associated with a poor prognosis in MM patients receiving intensive chemotherapy and autotransplant...
  31. ncbi request reprint Detection of chromosome 13q deletions and IgH translocations in patients with multiple myeloma by FISH: comparison with karyotype analysis
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, Toronto, Canada
    Leuk Lymphoma 45:965-9. 2004
    ..Since 13q deletions, IgH translocations and a hypodiploid karyotype are significant prognostic factors for MM, our study illustrates the importance of combining conventional cytogenetics with interphase FISH analysis in patients with MM...
  32. ncbi request reprint p53 gene deletion detected by fluorescence in situ hybridization is an adverse prognostic factor for patients with multiple myeloma following autologous stem cell transplantation
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, Toronto, ON, Canada
    Blood 105:358-60. 2005
    ..0009) or overall survival (P = .0002) in patients with MM after high-dose chemotherapy and autologous stem cell transplantation...
  33. ncbi request reprint Multiple myeloma involving central nervous system: high frequency of chromosome 17p13.1 (p53) deletions
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, ON, Canada
    Br J Haematol 127:280-4. 2004
    ..3 (FGFR3), the other involving 16q23 (c-maf). The high incidence of p53 deletions detected by cIg-FISH in CNS myeloma may be a marker for chromosomal instability, and may be associated with metastatic features of myeloma cells...
  34. doi request reprint Impact of genomic aberrations including chromosome 1 abnormalities on the outcome of patients with relapsed or refractory multiple myeloma treated with lenalidomide and dexamethasone
    Hong Chang
    Department of Laboratory Hematology, University Health Network, Toronto, Canada
    Leuk Lymphoma 51:2084-91. 2010
    ..Our data suggest that chromosome 17p and 1p21 deletions adversely impact the outcome of lenalidomide and dexamethasone treated patients with relapsed/refractory MM. Improved therapeutic strategies are required for these patients...
  35. ncbi request reprint MDM2 antagonist nutlin plus proteasome inhibitor velcade combination displays a synergistic anti-myeloma activity
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Hospital Research Institute and Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada
    Cancer Biol Ther 9:936-44. 2010
    ..These preclinical studies provide the framework for clinical trial of nutlin, alone and in combination with conventional and novel therapies such as velcade to increase efficacy and improve patient outcome in MM...
  36. ncbi request reprint Clinical outcomes in t(4;14) multiple myeloma: a chemotherapy-sensitive disease characterized by rapid relapse and alkylating agent resistance
    Wilfrid Jaksic
    Department of Medical Oncology and Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Canada
    J Clin Oncol 23:7069-73. 2005
    ..To determine whether primary drug resistance or rapid relapse explains the poor prognosis seen in t(4;14)-positive multiple myeloma (MM)...
  37. doi request reprint Influence of cytogenetics in patients with relapsed or refractory multiple myeloma treated with lenalidomide plus dexamethasone: adverse effect of deletion 17p13
    Donna Reece
    Division of Oncology, Princess Margaret Hospital, Toronto, Ontario, Canada
    Blood 114:522-5. 2009
    ..001) and median overall survival of 4.67 months (hazard ratio, 3.23; P < .001). Improved therapeutic strategies are required for this subgroup of patients. This study was registered at www.ClinicalTrials.gov as #NCT00179647...
  38. doi request reprint Cytoplasmic expression of nucleophosmin accurately predicts mutation in the nucleophosmin gene in patients with acute myeloid leukemia and normal karyotype
    Jennifer Luo
    Departments of 1Laboratory Hematology, University Health Network, and Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    Am J Clin Pathol 133:34-40. 2010
    ..Our data confirm that cytoplasmic NPM1 immunoreactivity predicts NPM1 mutations and warrants inclusion in the routine diagnostic and prognostic workup of AML...
  39. ncbi request reprint CHIR-258, a novel, multitargeted tyrosine kinase inhibitor for the potential treatment of t(4;14) multiple myeloma
    Suzanne Trudel
    Department of Medical Oncology, University Health Network, Princess Margaret Hospital and McLaughlin Centre of Molecular Medicine, University of Toronto, 620 University Ave, Rm 8 204, Toronto, ON, Canada M5G 2C1
    Blood 105:2941-8. 2005
    ..Finally, therapeutic efficacy of CHIR-258 was demonstrated in a xenograft mouse model of FGFR3 MM. These studies support the clinical evaluation of CHIR-258 in MM...
  40. doi request reprint Prognostic factors in normal karyotype acute myeloid leukemia in the absence of the FLT3-ITD mutation
    Allan Jiang
    Department of Laboratory Hematology, University Health Network, Toronto, ON, Canada
    Leuk Res 35:492-8. 2011
    ..045 and P=0.028, respectively), and CD56 positivity predicted shorter EFS (P=0.012). Our results confirm the favorable impact of NPM1 mutations and identify the adverse prognostic relevance of CD56 expression in this subgroup of AML...
  41. doi request reprint Analysis of chromosome 12p deletion in plasma cell dyscrasias
    Nan Jiang
    Department of Laboratory Hematology, University Health Network, Toronto, Canada
    Leuk Res 36:32-6. 2012
    ..Our results do not support the use of 12p deletion as a prognostic marker in MM, rather, it tends to occur in advanced disease, may represent a secondary change associated with the disease progression...
  42. doi request reprint Cyclin kinase subunit 1B nuclear expression predicts an adverse outcome for patients with relapsed/refractory multiple myeloma treated with bortezomib
    Mei Hsi Chen
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    Hum Pathol 43:858-64. 2012
    ..Our results indicated that cyclin kinase subunit 1B nuclear expression detected by immunohistochemistry is an adverse prognostic factor for patients with multiple myeloma treated with bortezomib therapy...
  43. doi request reprint p53 nuclear expression correlates with hemizygous TP53 deletion and predicts an adverse outcome for patients with relapsed/refractory multiple myeloma treated with lenalidomide
    Mei Hsi Chen
    Dept of Laboratory Hematology, University Health Network, University of Toronto, 200 Elizabeth Street, Toronto, Ontario, Canada
    Am J Clin Pathol 137:208-12. 2012
    ....
  44. ncbi request reprint Acute leukemia of donor origin arising after stem cell transplantation for acute promyelocytic leukemia
    Andrew S Daly
    Allogeneic Bone Marrow Transplant Service, Department of Medical Oncology and Hematology, Princess Margaret Hospital University Health Network, Toronto, Ont, Canada
    Leuk Res 28:1107-11. 2004
    ..To our knowledge, this is the first report of donor cell leukemia following BMT for APL. We hypothesize that replicative stress may lead to the development of some cases of donor cell acute leukemia...
  45. doi request reprint Adult precursor T-lymphoblastic leukemia/lymphoma with myeloid-associated antigen expression is associated with a lower complete remission rate following induction chemotherapy
    Murtadha Al Khabori
    Departments of Laboratory Hematology and Medical Oncology, University Health Network, Toronto, Ont, Canada
    Acta Haematol 120:5-10. 2008
    ..Our study indicates that expression of myeloid-associated antigens is associated with a lower CR rate in adult T-ALL and may be considered in risk stratification for induction chemotherapy...
  46. doi request reprint RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada
    Mol Cancer Ther 9:3041-51. 2010
    ..56 to 0.82 in MM cells. Our data support further clinical evaluation of RITA as a potential novel therapeutic intervention in MM...
  47. doi request reprint Treatment of adults with BCR-ABL negative acute lymphoblastic leukaemia with a modified paediatric regimen
    John M Storring
    Department of Medical Oncology and Haematology, Princess Margaret Hospital, University of Toronto, Toronto, ON, Canada
    Br J Haematol 146:76-85. 2009
    ..Effective delivery of asparaginase dosing appears to be important in achieving an optimal antileukaemic effect...
  48. doi request reprint CD34 expression predicts an adverse outcome in patients with NPM1-positive acute myeloid leukemia
    Harry Dang
    Department of Laboratory Hematology, University Health Network, Toronto, Ontario, Canada M5G 2C4 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5S 1A1
    Hum Pathol 44:2038-46. 2013
    ..027) in the FLT3-ITD-positive group. Our results confirm the prognostic value of assessing FLT3-ITD mutations in NPM1-positive AML and identify the adverse prognostic impact of high WBC and CD34 expression in this subgroup of AML. ..
  49. pmc Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Hospital Research Institute, Toronto, Ontario, Canada
    PLoS ONE 7:e30215. 2012
    ..Our study unveils a previously undescribed mechanism of RITA-induced p53-mediated apoptosis through JNK signaling pathway and provides the rationale for combination of p53 activating drugs with JNK activators in the treatment of MM...
  50. pmc Molecular mechanisms of nutlin-induced apoptosis in multiple myeloma: evidence for p53-transcription-dependent and -independent pathways
    Manujendra N Saha
    Division of Molecular and Cellular Biology, Toronto General Hospital Research Institute, Dept of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada
    Cancer Biol Ther 10:567-78. 2010
    ..Our study provides the evidence that nutlin-induced apoptosis in MM cells is mediated by transcription-dependent and -independent pathways and supports further clinical evaluation of nutlin as a novel therapeutic agent in MM...
  51. doi request reprint p53 nuclear accumulation is associated with extramedullary progression of multiple myeloma
    Neil Sheth
    Department of Laboratory Hematology, University Health Network, Toronto, ON, Canada
    Leuk Res 33:1357-60. 2009
    ..0001). Our data indicates that p53 nuclear expression but not CD56 expression, along with increased proliferation index is associated with disease progression from intramedullary to extramedullary sites in MM...
  52. ncbi request reprint T-cell large granular lymphocytic leukemia of donor origin occurring after allogeneic bone marrow transplantation for B-cell lymphoproliferative disorders
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, University of Toronto, ON, Canada
    Am J Clin Pathol 123:196-9. 2005
    ..These data show that T-LGL leukemia should be included as one of the types of posttransplantation lymphoproliferative disorders that can occur after allogeneic BMT for B-cell neoplasms...
  53. doi request reprint Genomic aberrations and survival of patients with light-chain-only multiple myeloma undergoing autologous stem cell transplantation
    Nan Jiang
    Department of Laboratory Haematology, Department of Medical Oncology and Haematology, University Health Network, University of Toronto, Toronto, Canada
    Biol Blood Marrow Transplant 17:1790-5. 2011
    ..9 vs 18.2 months, P = .001). There was no significant difference in PFS or OS in patients with or without t(4;14) or del(17p). On multivariate analysis, del(13q) was an independent prognostic factor for PFS and OS...
  54. doi request reprint Genomic stratification of multiple myeloma treated with novel agents
    Allan Jiang
    Department of Laboratory Hematology and Medical Oncology, University Health Network, Toronto, Ontario, Canada
    Leuk Lymphoma 53:202-7. 2012
    ..Here we review recent studies that analyze the impact of specific genomic aberrations on the outcome of MM treated with bortezomib and/or lenalidomide...
  55. ncbi request reprint Aberrant expression of T-cell-associated markers CD4 and CD7 on B-cell chronic lymphocytic leukemia
    Prashant Jani
    Department of Laboratory Hematology, Toronto General Hospital University Health Network, University of Toronto, Toronto, Canada
    Am J Hematol 82:73-6. 2007
    ....
  56. ncbi request reprint The inhibitory anti-FGFR3 antibody, PRO-001, is cytotoxic to t(4;14) multiple myeloma cells
    Suzanne Trudel
    University Health Network, Princess Margaret Hospital, McLaughlin Centre of Molecular Medicine, 620 University Avenue, Rm 8204, Toronto, Ontario, Canada M5G 2C1
    Blood 107:4039-46. 2006
    ..The data demonstrate that PRO-001 is a potent and specific inhibitor of FGFR3 and deserves further study for the treatment of FGFR3-expressing myeloma...
  57. doi request reprint Prognostic value of immunophenotyping and gene mutations in elderly patients with acute myeloid leukemia with normal karyotype
    Harry Dang
    Department of Laboratory Hematology, University Health Network, Toronto, Ontario, Canada
    Hum Pathol 44:55-61. 2013
    ..By combining genetic and immunophenotypic markers, we can divide patients into distinct prognostic groups with important implications for prognostic stratification and risk-adapted therapy...
  58. ncbi request reprint Acute myeloid leukemia with pseudo-Chèdiak-Higashi anomaly exhibits a specific immunophenotype with CD2 expression
    Hong Chang
    Department of Laboratory Hematology, Department of Laboratory Medicine and Pathobiology, University Health Network, University of Toronto, 610 University Avenue 4 320, Toronto, Ontario, Canada
    Am J Clin Pathol 125:791-4. 2006
    ..9%) of 140 cases of newly diagnosed AML without PCH anomaly. CD2 expression correlated strongly with AML with PCH anomaly (P < .01), suggesting a link between a specific immunophenotypic marker, CD2, and AML with PCH anomaly...
  59. ncbi request reprint The morphological subcategories of acute monocytic leukemia (M5a and M5b) share similar immunophenotypic and cytogenetic features and clinical outcomes
    Pierre Villeneuve
    Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, Canada
    Leuk Res 32:269-73. 2008
    ..Our data indicate that the prognosis of AML M5 is similar to non-M5 AML and that M5a and M5b do not differ in immunophenotype, cytogenetics or clinical outcome...
  60. ncbi request reprint Molecular characterization of chronic lymphocytic leukemia with two distinct cell populations: Evidence for separate clonal origins
    Hong Chang
    Department of Laboratory Hematology, University Health Network and University of Toronto, Toronto, Canada
    Am J Clin Pathol 126:23-8. 2006
    ..Our results demonstrated that the immunophenotypically different cell populations originated from 2 separate clones...
  61. doi request reprint Pharmacological activation of the p53 pathway in haematological malignancies
    Manujendra N Saha
    Division of Cellular and Molecular Biology, Toronto General Hospital Research Institute, University of Toronto, Canada
    J Clin Pathol 63:204-9. 2010
    ..This review will highlight recent findings in the research into molecules capable of modulating p53 protein activities and mechanisms that activate the p53 pathway, restoring response to therapy in haematological malignancies...
  62. doi request reprint CD13 expression is an independent adverse prognostic factor in adults with Philadelphia chromosome negative B cell acute lymphoblastic leukemia
    Kenneth J Craddock
    Department of Hematology and Pathology, University Health Network, Toronto, ON, Canada
    Leuk Res 37:759-64. 2013
    ..001), EFS (p=7×10(-4)) and RFS (p=8×10(-4)). Incorporating CD13 into a scoring system provides high discrimination for relapse risk and survival...
  63. ncbi request reprint Loss of Alx4, a stromally-restricted homeodomain protein, impairs mammary epithelial morphogenesis
    Purna A Joshi
    Department of Laboratory Medicine and Pathobiology, Room 6318, Medical Sciences Building, 1 King s College Circle, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Dev Biol 297:284-94. 2006
    ..Thus, the activity of the stromally restricted homeodomain factor, Alx4, is required for normal branching morphogenesis of the ductal epithelia during pubescent mammary gland development...
  64. pmc Natural killer or natural killer/T cell lineage large granular lymphocytosis associated with dasatinib therapy for Philadelphia chromosome positive leukemia
    Dong Hwan Kim
    Chronic Myelogenous Leukemia Group, Department of Hematology Medical Oncology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada
    Haematologica 94:135-9. 2009
    ....
  65. ncbi request reprint Preclinical studies of fibroblast growth factor receptor 3 as a therapeutic target in multiple myeloma
    Joshua L Paterson
    Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada
    Br J Haematol 124:595-603. 2004
    ..Myeloma cells lacking the t(4;14) or with the t(4;14) and a secondary RAS mutation did not respond to therapy. These findings support the development of clinical trials of early intervention with FGFR3 inhibitors in t(4;14) myeloma...
  66. pmc Activation of Erk by sonic hedgehog independent of canonical hedgehog signalling
    Hong Chang
    Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
    Int J Biochem Cell Biol 42:1462-71. 2010
    ..These data reveal novel binding activities for the C-terminal region of Ptch1 and define a signalling pathway stimulated by the Hh-ligands operating independently of pathways requiring Smo...
  67. doi request reprint No significance of derivative chromosome 9 deletion on the clearance kinetics of BCR/ABL fusion transcripts, cytogenetic or molecular response, loss of response, or treatment failure to imatinib mesylate therapy for chronic myeloid leukemia
    Dong Hwan Dennis Kim
    Chronic Myelogenous Leukemia Group, Department of Hematology Medical Oncology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada
    Cancer 113:772-81. 2008
    ....
  68. doi request reprint Aberrant nuclear p53 expression predicts hemizygous 17p (TP53) deletion in chronic lymphocytic leukemia
    Hong Chang
    Departments of 1Laboratory Hematology, Toronto General Hospital, University Health Network, Toronto, Canada
    Am J Clin Pathol 133:70-4. 2010
    ....
  69. doi request reprint CD11b expression correlates with monosomal karyotype and predicts an extremely poor prognosis in cytogenetically unfavorable acute myeloid leukemia
    Mei Hsi Chen
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Leuk Res 37:122-8. 2013
    ..Our proposed scoring model may be adapted in clinical practice to further the stratification of this high-risk population...
  70. doi request reprint A phase I study of the pan bcl-2 family inhibitor obatoclax mesylate in patients with advanced hematologic malignancies
    Aaron D Schimmer
    Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
    Clin Cancer Res 14:8295-301. 2008
    ..Therefore, we conducted a phase I clinical trial of the small-molecule pan-Bcl-2 inhibitor, obatoclax mesylate, in patients with refractory leukemia and myelodysplasia to assess its safety and define its optimal dose...
  71. doi request reprint A novel diquinolonium displays preclinical anti-cancer activity and induces caspase-independent cell death
    Rose Hurren
    Ontario Cancer Institute, Princess Margaret Hospital, 610 University Ave, Toronto, ON, Canada M5G 2M9
    Apoptosis 13:748-55. 2008
    ..Thus, Q(2) is a novel quinolinium with preclinical activity in malignancies such as leukemia and myeloma and warrants further investigation...
  72. ncbi request reprint Diagnosing PNH with FLAER and multiparameter flow cytometry
    D Robert Sutherland
    Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
    Cytometry B Clin Cytom 72:167-77. 2007
    ..Our goals were to develop a FLAER-based assay to diagnose and monitor patients with PNH and to improve detection of minor populations of PNH clones in other hematologic disorders...
  73. ncbi request reprint Hairy cell leukemia variant with features of intrasinusoidal bone marrow involvement
    Charin Ya-In
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Ontario, Canada
    Arch Pathol Lab Med 129:395-8. 2005
    ..This case illustrates an atypical marrow presentation of HCL-V and emphasizes the need to correlate all clinical and pathologic data, including tissue biopsy, in reaching a diagnosis...
  74. ncbi request reprint Complete remission of tumour with interleukin 2 therapy in a patient with non-Hodgkin's lymphoma post allogeneic bone marrow transplant associated with polyclonal T-cell bone marrow lymphocytosis
    Thomas L Kiss
    Bone Marrow Transplant Service, Department of Medical Oncology and Haematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Canada
    Br J Haematol 120:523-5. 2003
    ..The patient remains in complete remission 37 months following allogeneic BMT...
  75. ncbi request reprint Bone marrow aspirates as part of routine donor assessment for allogeneic blood and marrow transplantation can reveal presence of occult hematological malignancies in otherwise asymptomatic individuals
    T L Kiss
    Department of Medical Oncology and Hematology, Princess Margaret Hospital University Health Network, Toronto, Ontario, Canada
    Bone Marrow Transplant 33:855-8. 2004
    ..Clonogenic assays can contribute to detect hemopoietic abnormalities pre transplant...
  76. ncbi request reprint Comparative analysis of tropism between canarypox (ALVAC) and vaccinia viruses reveals a more restricted and preferential tropism of ALVAC for human cells of the monocytic lineage
    Qigui Yu
    Hawaii AIDS Clinical Research Program, Department of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA
    Vaccine 24:6376-91. 2006
    ..These findings have implications in terms of choosing optimal pox virus derived vectors as vaccines in terms of reducing clinical reactogenicity and inducing dendritic cell (DC) maturation...
  77. ncbi request reprint CEP-7055: a novel, orally active pan inhibitor of vascular endothelial growth factor receptor tyrosine kinases with potent antiangiogenic activity and antitumor efficacy in preclinical models
    Bruce Ruggeri
    Department of Oncology, Cephalon, Inc, West Chester, Pennsylvania 19380, USA
    Cancer Res 63:5978-91. 2003
    ..Orally administered CEP-7055 has entered Phase I clinical trials in cancer patients...
  78. ncbi request reprint Human IgG1 Cgamma1 domain is crucial for the bioactivity of the engineered anti-CD20 antibodies
    Shusheng Geng
    College of Life Science, Hebei University, Baoding, China
    Cell Mol Immunol 4:121-5. 2007
    ..Our study highlights some interests and advantages of a methodology based on the homology modeling and analysis of molecular structural properties...
  79. ncbi request reprint Structure-guided identification of novel VEGFR-2 kinase inhibitors via solution phase parallel synthesis
    Rabindranath Tripathy
    Cephalon, Inc, Discovery Research, 145 Brandywine Parkway, West Chester, PA 19380, USA
    Bioorg Med Chem Lett 16:2158-62. 2006
    ....
  80. ncbi request reprint Basigin expression and hormonal regulation in mouse uterus during the peri-implantation period
    Li Juan Xiao
    College of Life Science, Northeast Agricultural University, Harbin, China
    Mol Reprod Dev 63:47-54. 2002
    ..A high level of basigin immunostaining and mRNA was also seen in proestrus and estrus uteri. These results suggest that basigin expression is closely related to mouse implantation and up-regulated by estrogen...
  81. ncbi request reprint Acute promyelocytic leukemia in patients aged 70 years and over -- a single center experience of unselected patients
    Patricia Disperati
    Leuk Lymphoma 48:1654-8. 2007
  82. ncbi request reprint The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice
    Sheila J Miknyoczki
    Cephalon, Inc, 145 Brandywine Parkway, West Chester, PA 19380, USA
    Clin Cancer Res 8:1924-31. 2002
    ..We examined the antitumor efficacy of administration of NT neutralizing antibodies on the growth of established human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice...
  83. ncbi request reprint c-Maf nuclear oncoprotein is frequently expressed in multiple myeloma
    Hong Chang
    Leukemia 21:1572-4. 2007
  84. ncbi request reprint Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist
    Pawel Dobrzanski
    Division of Oncology, Cephalon, Inc, West Chester, Pennsylvania, USA
    Cancer Res 64:910-9. 2004
    ..These data demonstrate a critical role of EphA signaling in tumor growth and metastasis and provide a strong rationale for targeting EphA2 receptors for anticancer therapies...
  85. ncbi request reprint Novel C-3 N-urea, amide, and carbamate dihydroindazolo[5,4-a]pyrrolo[3,4-c]carbazole analogs as potent TIE-2 and VEGF-R2 dual inhibitors
    Nadine C Becknell
    Cephalon, Inc, 145 Brandywine Parkway, West Chester, PA 19380, USA
    Bioorg Med Chem Lett 16:5368-72. 2006
    ..The isopropyl carbamate 39 displayed good dual enzyme, cell potency, and rat pharmacokinetic properties for advancement to in vivo evaluation...
  86. ncbi request reprint Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum
    Hong Chang
    College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
    Mol Reprod Dev 68:135-41. 2004
    ..However, no basigin expression was detected from days 6 to 15 post-hCG injection. In conclusion, our data suggest that basigin may play a role during the mouse follicle development and corpus luteum formation...
  87. ncbi request reprint Karyotype stability of the DT40 chicken B cell line: macrochromosome variation and cytogenetic mosaicism
    Hong Chang
    Department of Animal Science, 2131D Meyer Hall, One Shields Avenue, University of California, Davis, CA 95616, USA
    Chromosome Res 12:299-307. 2004
    ..The results are discussed in regard to current knowledge of cytogenetic anomalies associated with chicken cancers...
  88. pmc Paternal germline origin and sex-ratio distortion in transmission of PTPN11 mutations in Noonan syndrome
    Marco Tartaglia
    Dipartimento di Biologia Cellulare e Neuroscienze, Istituto Superiore di Sanita, Rome, Italy
    Am J Hum Genet 75:492-7. 2004
    ....
  89. ncbi request reprint [Subcloning of human neurotrophin-3 gene and construction of its genetically engineered cell model]
    Hong Chang
    Department of Otolaryngology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China
    Di Yi Jun Yi Da Xue Xue Bao 24:805-8. 2004
    ..To subclone human neurotrophin-3 gene (NT3) and transfer this gene into human bone marrow mesenchymal stem cells (BM-MSCs) to construct genetically engineered cells that produce NT3 in vitro...
  90. ncbi request reprint Relationship between all-cause mortality and cumulative working life course psychosocial and physical exposures in the United States labor market from 1968 to 1992
    Benjamin C Amick
    Center for Society and Population Health, University of Texas School of Public Health, Houston, Texas, USA
    Psychosom Med 64:370-81. 2002
    ..To examine the relationship between cumulative exposures to psychosocial and physical work conditions and mortality in a nationally representative sample...
  91. ncbi request reprint [Studies of hereditary conditionality index on some morphological characters of Tupaia belangeri chinensis]
    Yong Mei Yu
    Shanghai Cancer Institute, Shanghai 200032, China
    Yi Chuan 26:478-80. 2004
    ....
  92. ncbi request reprint The selective poly(ADP-ribose) polymerase-1(2) inhibitor, CEP-8983, increases the sensitivity of chemoresistant tumor cells to temozolomide and irinotecan but does not potentiate myelotoxicity
    Sheila Miknyoczki
    Cephalon, Inc, 145 Brandywine Parkway, West Chester, PA 19380, USA
    Mol Cancer Ther 6:2290-302. 2007
    ..These data indicate that CEP-8983 and its prodrug are effective chemosensitizing agents when administered in combination with select chemotherapeutic agents against chemoresistant tumors...