Ann F Chambers

Summary

Affiliation: University of Western Ontario
Country: Canada

Publications

  1. ncbi request reprint Ovarian cancer biomarkers in urine
    Ann F Chambers
    Department of Oncology, University of Western Ontario, London Regional Cancer Program, London, Ontario, Canada
    Clin Cancer Res 12:323-7. 2006
  2. ncbi request reprint Molecular mechanisms of metastasis
    Dave S B Hoon
    Department of Molecular Oncology, John Wayne Cancer Institute, Santa Monica, CA 90404, USA
    Cancer Metastasis Rev 25:203-20. 2006
  3. pmc Breast cancer metastasis suppressor 1 (BRMS1) inhibits osteopontin transcription by abrogating NF-kappaB activation
    Rajeev S Samant
    Mitchell Cancer Institute, University of South Alabama, Mobile, Alabama, USA
    Mol Cancer 6:6. 2007
  4. doi request reprint MDA-MB-435 and M14 cell lines: identical but not M14 melanoma?
    Ann F Chambers
    London Regional Cancer Program, Department of Oncology, University of Western Ontario, London, Ontario, Canada
    Cancer Res 69:5292-3. 2009
  5. doi request reprint Influence of diet on metastasis and tumor dormancy
    Ann F Chambers
    Department of Oncology, University of Western Ontario, London, ON, Canada
    Clin Exp Metastasis 26:61-6. 2009
  6. doi request reprint Downregulation of osteopontin contributes to metastasis suppression by breast cancer metastasis suppressor 1
    Benjamin D Hedley
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Int J Cancer 123:526-34. 2008
  7. ncbi request reprint Human 21T breast epithelial cell lines mimic breast cancer progression in vivo and in vitro and show stage-specific gene expression patterns
    Lesley H Souter
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Lab Invest 90:1247-58. 2010
  8. ncbi request reprint A new model for lymphatic metastasis: development of a variant of the MDA-MB-468 human breast cancer cell line that aggressively metastasizes to lymph nodes
    Sharon A Vantyghem
    London Regional Cancer Program, 790 Commissioners Road East, Ontario, N6A 4L6, London, Canada
    Clin Exp Metastasis 22:351-61. 2005
  9. doi request reprint BRMS1 suppresses breast cancer metastasis in multiple experimental models of metastasis by reducing solitary cell survival and inhibiting growth initiation
    Benjamin D Hedley
    London Regional Cancer Program, London Health Sciences Centre, 790 Commissioners Road East, London, ON, Canada N6A 4L6
    Clin Exp Metastasis 25:727-40. 2008
  10. doi request reprint Three-dimensional imaging and quantification of both solitary cells and metastases in whole mouse liver by magnetic resonance imaging
    Jason L Townson
    University of Western Ontario, London, Ontario, Canada
    Cancer Res 69:8326-31. 2009

Detail Information

Publications95

  1. ncbi request reprint Ovarian cancer biomarkers in urine
    Ann F Chambers
    Department of Oncology, University of Western Ontario, London Regional Cancer Program, London, Ontario, Canada
    Clin Cancer Res 12:323-7. 2006
  2. ncbi request reprint Molecular mechanisms of metastasis
    Dave S B Hoon
    Department of Molecular Oncology, John Wayne Cancer Institute, Santa Monica, CA 90404, USA
    Cancer Metastasis Rev 25:203-20. 2006
    ..The hope is that further unraveling of the direct and indirect molecular events of lymphatic metastasis will lead to new approaches in developing effective therapeutics...
  3. pmc Breast cancer metastasis suppressor 1 (BRMS1) inhibits osteopontin transcription by abrogating NF-kappaB activation
    Rajeev S Samant
    Mitchell Cancer Institute, University of South Alabama, Mobile, Alabama, USA
    Mol Cancer 6:6. 2007
    ..BRMS1 is a member of mSin3-HDAC transcription co-repressor complex and has been shown to suppress the metastasis of breast cancer and melanoma cells in animal models. Hence we hypothesized that BRMS1 regulates OPN expression...
  4. doi request reprint MDA-MB-435 and M14 cell lines: identical but not M14 melanoma?
    Ann F Chambers
    London Regional Cancer Program, Department of Oncology, University of Western Ontario, London, Ontario, Canada
    Cancer Res 69:5292-3. 2009
    ..Evidence from the literature is presented here that is inconsistent with both lines being of M14 melanoma origin, but rather is consistent with both cell lines being of MDA-MB-435 breast cancer origin...
  5. doi request reprint Influence of diet on metastasis and tumor dormancy
    Ann F Chambers
    Department of Oncology, University of Western Ontario, London, ON, Canada
    Clin Exp Metastasis 26:61-6. 2009
    ....
  6. doi request reprint Downregulation of osteopontin contributes to metastasis suppression by breast cancer metastasis suppressor 1
    Benjamin D Hedley
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Int J Cancer 123:526-34. 2008
    ..These studies clarify one mechanism by which BRMS1 can suppress metastasis...
  7. ncbi request reprint Human 21T breast epithelial cell lines mimic breast cancer progression in vivo and in vitro and show stage-specific gene expression patterns
    Lesley H Souter
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Lab Invest 90:1247-58. 2010
    ..This system thus reveals potential therapeutic targets and will provide a means of testing the influences of identified genes on transitions between these stages of pre-malignant to malignant growth...
  8. ncbi request reprint A new model for lymphatic metastasis: development of a variant of the MDA-MB-468 human breast cancer cell line that aggressively metastasizes to lymph nodes
    Sharon A Vantyghem
    London Regional Cancer Program, 790 Commissioners Road East, Ontario, N6A 4L6, London, Canada
    Clin Exp Metastasis 22:351-61. 2005
    ..The dramatic in vitro and in vivo phenotypic and molecular differences of 468LN and parental 468GFP cells make this pair of cell lines a unique model for the specific study of lymph node metastasis of breast cancer...
  9. doi request reprint BRMS1 suppresses breast cancer metastasis in multiple experimental models of metastasis by reducing solitary cell survival and inhibiting growth initiation
    Benjamin D Hedley
    London Regional Cancer Program, London Health Sciences Centre, 790 Commissioners Road East, London, ON, Canada N6A 4L6
    Clin Exp Metastasis 25:727-40. 2008
    ..These novel findings demonstrate that BRMS1 is a potent suppressor of metastasis in multiple organs, and identify two steps in the metastatic process that are sensitive to inhibition by BRMS1...
  10. doi request reprint Three-dimensional imaging and quantification of both solitary cells and metastases in whole mouse liver by magnetic resonance imaging
    Jason L Townson
    University of Western Ontario, London, Ontario, Canada
    Cancer Res 69:8326-31. 2009
    ....
  11. pmc Multi-platform whole-genome microarray analyses refine the epigenetic signature of breast cancer metastasis with gene expression and copy number
    Joseph Andrews
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    PLoS ONE 5:e8665. 2010
    ....
  12. ncbi request reprint Three-dimensional high-frequency ultrasound imaging for longitudinal evaluation of liver metastases in preclinical models
    Kevin C Graham
    Department of Medical Biophysics, The University of Western Ontario, London, Canada
    Cancer Res 65:5231-7. 2005
    ....
  13. ncbi request reprint In vivo videomicroscopy reveals differential effects of the vascular-targeting agent ZD6126 and the anti-angiogenic agent ZD6474 on vascular function in a liver metastasis model
    Hemanth J Varghese
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    Angiogenesis 7:157-64. 2004
    ..Understanding differences in the modes of action of VTAs and anti-angiogenic agents will be important in optimizing their clinical application and in developing appropriate combination strategies...
  14. ncbi request reprint Dietary genistein reduces metastasis in a postsurgical orthotopic breast cancer model
    Sharon A Vantyghem
    Department of Pathology, University of Western Ontario, Canada
    Cancer Res 65:3396-403. 2005
    ..The availability of well-characterized, clinically relevant animal models for studying factors that regulate metastatic outgrowth postsurgery will provide an important tool for developing new systemic adjuvant therapies...
  15. pmc Role of the integrin-binding protein osteopontin in lymphatic metastasis of breast cancer
    Alison L Allan
    Department of Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, London Regional Cancer Program, 790 Commissioners Road East, London, Ontario N6A 4L6, Canada
    Am J Pathol 169:233-46. 2006
    ....
  16. doi request reprint Noninvasive quantification of tumor volume in preclinical liver metastasis models using contrast-enhanced x-ray computed tomography
    Kevin C Graham
    Department of Medical Biophysics, The University of Western Ontario, London, Ontario, Canada
    Invest Radiol 43:92-9. 2008
    ..To evaluate the utility of images acquired during this time period for the noninvasive measurement of liver-tumor volume...
  17. pmc Notch1 inhibition alters the CD44hi/CD24lo population and reduces the formation of brain metastases from breast cancer
    Patricia M McGowan
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    Mol Cancer Res 9:834-44. 2011
    ..001; Kruskal-Wallis). These data suggest that the CSC phenotype contributes to the development of brain metastases from breast cancer, and this may arise in part from increased Notch activity...
  18. pmc Epigenetic mapping and functional analysis in a breast cancer metastasis model using whole-genome promoter tiling microarrays
    David I Rodenhiser
    London Regional Cancer Program, Victoria Research Laboratories, London Health Sciences Centre, 790 Commissioners Road East, London, Ontario, N6A 4L6, Canada
    Breast Cancer Res 10:R62. 2008
    ..Furthermore, no genome-wide assessments have been undertaken to identify altered DNA methylation patterns in the context of metastasis and their effects on specific functional pathways or gene networks...
  19. doi request reprint Distinct karyotypes in three breast cancer cell lines --21PTCi, 21NTCi, and 21MT-1 --derived from the same patient and representing different stages of tumor progression
    Jie Xu
    Cytogenetics Laboratory, London Health Sciences Centre, University of Western Ontario, London, Ontario, Canada
    Cancer Genet Cytogenet 186:33-40. 2008
    ..These three cell lines, with their different karyotypes and biologic characteristics, provide a vital tool for further study of the genetic and epigenetic events involved in transitions between premalignant and malignant phenotypes...
  20. ncbi request reprint In vivo inhibition of growth of human tumor lines by flavonoid fractions from cranberry extract
    Peter J Ferguson
    London Regional Cancer Program, Health Sciences Centre, 790 Commissioners Road East, London, Ontario, Canada
    Nutr Cancer 56:86-94. 2006
    ..05) and also induce cell death within 24 to 48 h of exposure (P < 0.05). These results indicate the presence of a potential anticancer constituent in the flavonoid-containing fractions from cranberry extracts...
  21. ncbi request reprint Lymphatic metastasis of breast cancer cells is associated with differential gene expression profiles that predict cancer stem cell-like properties and the ability to survive, establish and grow in a foreign environment
    Terlika S Pandit
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Int J Oncol 35:297-308. 2009
    ..Differentially expressed genes which reflect these functions provide candidates for investigation as potential targets for therapy directed against early lymphatic metastasis...
  22. doi request reprint Contrast-enhanced microcomputed tomography using intraperitoneal contrast injection for the assessment of tumor-burden in liver metastasis models
    Kevin C Graham
    Department of Medical Biophysics, The University of Western Ontario, London, Ontario, Canada
    Invest Radiol 43:488-95. 2008
    ....
  23. ncbi request reprint Beta(3) integrin expression increases breast carcinoma cell responsiveness to the malignancy-enhancing effects of osteopontin
    Kara A Furger
    Department of Oncology, University of Western Ontario, Ontario, Canada
    Mol Cancer Res 1:810-9. 2003
    ....
  24. ncbi request reprint The thrombin inhibitor Argatroban reduces breast cancer malignancy and metastasis via osteopontin-dependent and osteopontin-independent mechanisms
    Erika B Schulze
    London Regional Cancer Program, London Health Sciences Centre, London, ON, Canada
    Breast Cancer Res Treat 112:243-54. 2008
    ....
  25. ncbi request reprint Osteopontin induction of hyaluronan synthase 2 expression promotes breast cancer malignancy
    Amy C Cook
    Department of Pathology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 281:24381-9. 2006
    ....
  26. ncbi request reprint Detection and quantification of circulating tumor cells in mouse models of human breast cancer using immunomagnetic enrichment and multiparameter flow cytometry
    Alison L Allan
    London Regional Cancer Program, London, Ontario, Canada
    Cytometry A 65:4-14. 2005
    ..The purpose of this study was to optimize the sensitivity of multiparameter flow cytometry for detection of human tumor cells in mouse models of breast cancer...
  27. ncbi request reprint In vivo MRI of cancer cell fate at the single-cell level in a mouse model of breast cancer metastasis to the brain
    Chris Heyn
    Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada
    Magn Reson Med 56:1001-10. 2006
    ....
  28. ncbi request reprint A new breast cancer model for lymphatic metastasis
    Michael M Lizardo
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    Cancer Treat Res 135:157-65. 2007
  29. ncbi request reprint Dormancy of solitary metastatic cells
    Jason L Townson
    Department of Medical Biophysics, University of Western Ontario and the London Regional Cancer Program, London, Ontario, Canada
    Cell Cycle 5:1744-50. 2006
    ....
  30. doi request reprint Effect of anti-fibrinolytic therapy on experimental melanoma metastasis
    Jennifer M Kirstein
    London Regional Cancer Program, London Health Sciences Centre, 790 Commissioners Road East, London, ON, Canada N6A 4L6
    Clin Exp Metastasis 26:121-31. 2009
    ....
  31. doi request reprint The synthetic triterpenoid CDDO-Imidazolide suppresses experimental liver metastasis
    Jason L Townson
    London Regional Cancer Program, 790 Commissioners Road East, Office A4 903B, London, ON N6A 4L6, Canada
    Clin Exp Metastasis 28:309-17. 2011
    ..9). This study demonstrates that CDDO-Im may be useful for the treatment metastatic liver disease as it successfully inhibits growth of actively proliferating liver metastases...
  32. doi request reprint Experimental metastasis assays in the chick embryo
    Sylvia M Wilson
    London Regional Cancer Centre, London, Ontario, Canada
    Curr Protoc Cell Biol . 2004
    ..These assays provide a cost-effective, readily accessible, and rapid approach for studying the process of cancer metastasis...
  33. pmc In vivo single scan detection of both iron-labeled cells and breast cancer metastases in the mouse brain using balanced steady-state free precession imaging at 1.5 T
    Emeline J Ribot
    Imaging Research Laboratories, Robarts Research Institute, London, ON, Canada
    J Magn Reson Imaging 34:231-8. 2011
    ..5 T on mice developing brain metastases subsequent to the injection of micron-sized iron oxide particle-labeled human breast cancer cells...
  34. doi request reprint New dual monoclonal ELISA for measuring plasma osteopontin as a biomarker associated with survival in prostate cancer: clinical validation and comparison of multiple ELISAs
    Pieter H Anborgh
    London Regional Cancer Program, London, Ontario, Canada
    Clin Chem 55:895-903. 2009
    ..We compared both assays with 4 assays that recognize defined regions of OPN protein (dual polyclonal systems 5-1, 4-1, 4-3 and polyclonal-monoclonal system 1-3)...
  35. pmc Gene signatures of breast cancer progression and metastasis
    David I Rodenhiser
    London Regional Cancer Program, Cancer Research Laboratories, London Health Sciences Centre, 790 Commissioners Road East, London, Ontario, N6A 4L6, Canada
    Breast Cancer Res 13:201. 2011
    ..The challenge is to learn what information leads to improved prognostic accuracy and treatment outcome for individual patients...
  36. pmc Co-expression of α9β1 integrin and VEGF-D confers lymphatic metastatic ability to a human breast cancer cell line MDA-MB-468LN
    Mousumi Majumder
    Department of Anatomy and Cell Biology, The University of Western Ontario, London, Ontario, Canada
    PLoS ONE 7:e35094. 2012
    ....
  37. doi request reprint Osteopontin increases breast cancer cell sensitivity to specific signaling pathway inhibitors in preclinical models
    Jennifer C Mutrie
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    Cancer Biol Ther 12:680-90. 2011
    ..These results suggest that OPN could potentially be useful clinically as a predictive marker in identifying patients who will benefit from either Hsp90 or Src kinase inhibitor therapy...
  38. doi request reprint In vivo characterization of changing blood-tumor barrier permeability in a mouse model of breast cancer metastasis: a complementary magnetic resonance imaging approach
    Dean B Percy
    Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada
    Invest Radiol 46:718-25. 2011
    ....
  39. ncbi request reprint Expression of osteopontin and HGF/met in adult soft tissue tumors
    Vivien H C Bramwell
    London Regional Cancer Program and Department of Pathology, London Health Sciences Centre, London, Ontario, Canada
    Cancer Biol Ther 4:1336-41. 2005
    ....
  40. doi request reprint Ex-vivo cellular MRI with b-SSFP: quantitative benefits of 3T over 1.5 T
    Soha Said Ramadan
    Imaging Research Laboratories, Robarts Research Institute, London, ON, Canada
    MAGMA 21:251-9. 2008
    ..We have recently reported that single iron-labeled cells could be detected, as signal voids, in vivo in mouse brains using a balanced steady-state free precession imaging sequence (b-SSFP) and a customized microimaging system at 1.5 T...
  41. ncbi request reprint Tumor dormancy and the role of metastasis suppressor genes in regulating ectopic growth
    Benjamin D Hedley
    University of Western Ontario, Schulich School of Medicine and Dentistry, London Regional Cancer Program, Department of Oncology, London, Ontario, Canada
    Future Oncol 2:627-41. 2006
    ....
  42. ncbi request reprint Changes over time of extracellular domain of HER2 (ECD/HER2) serum levels have prognostic value in metastatic breast cancer
    Vivien H C Bramwell
    London Regional Cancer Program, London, ON, Canada
    Breast Cancer Res Treat 114:503-11. 2009
    ..Our aim was to assess the prognostic value of baseline levels of ECD/HER2, but more importantly changes in levels over time, in women with metastatic breast cancer...
  43. doi request reprint An emerging role for the nuclear localization of maspin in the suppression of tumor progression and metastasis
    Brigitte Goulet
    London Regional Cancer Program, Translational Prostate Cancer Research Group, London, ON N6A 4L6, Canada
    Biochem Cell Biol 90:22-38. 2012
    ..Elucidation of the molecular mechanisms regulating the localization and activities of maspin should pave the way for the development of improved diagnostics and therapies for cancer...
  44. pmc In Vivo Magnetic Resonance Imaging for Investigating the Development and Distribution of Experimental Brain Metastases due to Breast Cancer
    Mevan Perera
    Robarts Research Institute, London, Ontario, Canada
    Transl Oncol 5:217-25. 2012
    ..The overall goal of this study was to assess the utility of three-dimensional magnetic resonance imaging (MRI) for monitoring the temporal and spatial development of experimental brain metastasis in mice...
  45. doi request reprint Role of the metastasis-promoting protein osteopontin in the tumour microenvironment
    Pieter H Anborgh
    London Regional Cancer Program, London, Ontario, Canada Department of Pathology, University of Western Ontario, London, Ontario, Canada
    J Cell Mol Med 14:2037-44. 2010
    ..Understanding OPN-mediated interactions within a tumour will be important for the development of therapeutic strategies to target OPN...
  46. ncbi request reprint Osteopontin induces multiple changes in gene expression that reflect the six "hallmarks of cancer" in a model of breast cancer progression
    Amy C Cook
    Department of Pathology, The University of Western Ontario, London, Ontario, Canada
    Mol Carcinog 43:225-36. 2005
    ..This study provides the first evidence that OPN can lead to numerous gene expression changes that influence multiple aspects of tumor progression and malignant growth...
  47. ncbi request reprint Breast cancer metastasis progression as revealed by intravital videomicroscopy
    Ian C MacDonald
    Department of Medical Biophysics, University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5C1, Canada
    Expert Rev Anticancer Ther 6:1271-9. 2006
    ....
  48. ncbi request reprint Tumor dormancy and cancer stem cells: implications for the biology and treatment of breast cancer metastasis
    Alison L Allan
    London Regional Cancer Program, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Breast Dis 26:87-98. 2006
    ....
  49. doi request reprint Molecular cytogenetic characterization of human breast cancer cell line MDA-MB-468 and its variant 468LN, which displays aggressive lymphatic metastasis
    Jie Xu
    Cytogenetics Laboratory, London Health Sciences Centre, University of Western Ontario, 375 South Street, Room N258, London, Ontario N6A 4G5, Canada
    Cancer Genet Cytogenet 181:1-7. 2008
    ..Knowledge of the chromosome aberrations and breakpoints identified could be useful for further genetic and epigenetic studies of breast cancer...
  50. pmc Imaging the impact of chemically inducible proteins on cellular dynamics in vivo
    Hon S Leong
    Translational Prostate Cancer Research Group, London Regional Cancer Program, London, Ontario, Canada
    PLoS ONE 7:e30177. 2012
    ..This technique allows for precise control over protein activity in vivo while permitting the temporal analysis of dynamic biophysical parameters...
  51. doi request reprint Nuclear localization of maspin is essential for its inhibition of tumor growth and metastasis
    Brigitte Goulet
    London Regional Cancer Program, London, Ontario, Canada
    Lab Invest 91:1181-7. 2011
    ....
  52. ncbi request reprint Ineffectiveness of doxorubicin treatment on solitary dormant mammary carcinoma cells or late-developing metastases
    George N Naumov
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    Breast Cancer Res Treat 82:199-206. 2003
    ..This study has important clinical implications for patients being treated with cytotoxic chemotherapy...
  53. ncbi request reprint In vivo magnetic resonance imaging of single cells in mouse brain with optical validation
    Chris Heyn
    Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada
    Magn Reson Med 55:23-9. 2006
    ..This work demonstrates the sensitivity of MRI for detecting single cells in small animals for a wide range of application from stem cell to cancer cell tracking...
  54. ncbi request reprint Time-course characterization of the computed tomography contrast enhancement of an iodinated blood-pool contrast agent in mice using a volumetric flat-panel equipped computed tomography scanner
    Nancy L Ford
    Imaging Research Laboratories, Robarts Research Institute Department of Medical Biophysics, University of Western Ontario, Canada
    Invest Radiol 41:384-90. 2006
    ..The objective of this study was to determine the time-course of computed tomography (CT) contrast enhancement of an iodinated blood-pool contrast agent...
  55. ncbi request reprint Osteopontin overexpression in breast cancer: knowledge gained and possible implications for clinical management
    Alan B Tuck
    London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada
    J Cell Biochem 102:859-68. 2007
    ..This review will focus on the role of OPN in breast cancer, in particular on the malignancy-promoting aspects of OPN that may reveal opportunities for new approaches to the clinical management of breast cancer...
  56. ncbi request reprint Myxoma virus oncolysis of primary and metastatic B16F10 mouse tumors in vivo
    Marianne M Stanford
    BioTherapeutics Research Group, Robarts Research Institute, University of Western Ontario, London, Ontario, Canada
    Mol Ther 16:52-9. 2008
    ..These results show MV to be a promising virotherapeutic agent in immunocompetent animal tumor models, with good efficacy in combination with rapamycin...
  57. ncbi request reprint Therapeutic targets for antimetastatic therapy
    Benjamin D Hedley
    Department of Medical Biophysics, University of Western Ontario, London Regional Cancer Program, London Health Sciences Centre, Ontario, N6A 4L6, Canada
    Expert Opin Ther Targets 8:527-36. 2004
    ..This review discusses potential biological targets, as defined by the steps in the metastatic process, for antimetastatic therapies and provides examples of clinical strategies for preventing or treating successful metastasis...
  58. pmc Tumour dormancy in breast cancer: an update
    Muriel Brackstone
    London Regional Cancer Program, London, Ontario, N6A 4L6 Canada
    Breast Cancer Res 9:208. 2007
    ..Current knowledge gaps are highlighted and potential areas of future research are identified...
  59. doi request reprint Chapter 9. Intravital videomicroscopy in angiogenesis research
    Ian C MacDonald
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada
    Methods Enzymol 444:201-30. 2008
    ..In this chapter, we describe methods and analytical approaches for using IVVM to study vascular morphology and dynamics...
  60. doi request reprint Tumor dormancy and metastasis
    Benjamin D Hedley
    Division of Hematology, London Health Sciences Centre, London, Ontario, Canada
    Adv Cancer Res 102:67-101. 2009
    ..An improved understanding of tumor dormancy is needed for better management of patients at risk for late-developing metastases...
  61. ncbi request reprint Plasma osteopontin levels are predictive of disease stage in patients with transitional cell carcinoma of the bladder
    Celina Ang
    Department of Surgery, University of Western Ontario, London, Ontario, Canada
    BJU Int 96:803-5. 2005
    ..To measure plasma levels of osteopontin in patients with transitional cell carcinoma (TCC) of the bladder, and to determine if osteopontin levels relate to disease stage...
  62. ncbi request reprint A Method to Validate Quantitative High-Frequency Power Doppler Ultrasound With Fluorescence in Vivo Video Microscopy
    Stephen Z Pinter
    Biomedical Engineering Graduate Program, Western University, London, Ontario, Canada Robarts Research Institute, Western University, London, Ontario, Canada
    Ultrasound Med Biol 40:1908-17. 2014
    ..An investigator can implement the WFSC method to help adapt cutoff velocity to current blood flow conditions and thereby improve the accuracy of power Doppler for quantitative microvascular imaging. ..
  63. doi request reprint Assessing cancer cell migration and metastatic growth in vivo in the chick embryo using fluorescence intravital imaging
    Hon Sing Leong
    The London Regional Cancer Center, London, ON, Canada
    Methods Mol Biol 872:1-14. 2012
    ..Here, we present recent developments of detailed procedures for using shell-less chick embryos, coupled with fluorescent labeling of cancer cells and/or chick vasculature, to study cancer cell migration and metastasis in vivo...
  64. pmc Pre- and post-translational regulation of osteopontin in cancer
    Pieter H Anborgh
    London Regional Cancer Program, 790 Commissioners Road East, London, ON, N6A 4L6, Canada
    J Cell Commun Signal 5:111-22. 2011
    ..The pattern of isoform expression and post-translational modification is cell-type specific and may influence the potential role of OPN in malignancy and as a cancer biomarker...
  65. doi request reprint Micrometastatic disease and metastatic outgrowth: clinical issues and experimental approaches
    Patricia M McGowan
    Department Medical Biophysics, University of Western Ontario, London, ON, Canada
    Future Oncol 5:1083-98. 2009
    ..Elucidation of the molecular pathways involved in dormancy will advance clinical understanding and may suggest new avenues for treatment to inhibit the revival of these dormant cells, thereby reducing cancer mortality rates...
  66. ncbi request reprint A flavonoid fraction from cranberry extract inhibits proliferation of human tumor cell lines
    Peter J Ferguson
    Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada
    J Nutr 134:1529-35. 2004
    ..05) and induce cells to undergo apoptosis (P < 0.05) in a dose-dependent manner. Fr6 is potentially a source of a novel anticancer agent...
  67. doi request reprint Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line
    Philip J Medeiros
    Department of Medical Biophysics, The University of Western Ontario, London, Ontario, Canada
    Int J Cancer 131:276-86. 2012
    ..These data are in congruence with those from human cell lines and highlight the 4T1 cell line as a translatable model of breast cancer in which the effects of NPY can be studied in an immunocompetent system...
  68. ncbi request reprint Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS): a new proteomic urinary test for patients with urolithiasis
    Peter A Cadieux
    Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, London, Ontario, Canada
    J Clin Lab Anal 18:170-5. 2004
    ..Although SELDI-TOF-MS is not yet in widespread use in hospital and diagnostic laboratories, this system represents a promising new method for rapidly identifying patients with urolithiasis...
  69. ncbi request reprint Mapping of the functional microcirculation in vital organs using contrast-enhanced in vivo video microscopy
    Hemanth J Varghese
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Am J Physiol Heart Circ Physiol 288:H185-93. 2005
    ..This study demonstrates that postprocessing of fluorescence contrast video sequences of the microcirculation can provide quantitative images useful for studies in a wide range of model systems...
  70. ncbi request reprint The role of apoptosis in tumor progression and metastasis
    Jason L Townson
    Department of Medical Biophysics, University of Western Ontario, London, Canada N6A 4L6
    Curr Mol Med 3:631-42. 2003
    ....
  71. ncbi request reprint Osteopontin-induced migration of human mammary epithelial cells involves activation of EGF receptor and multiple signal transduction pathways
    Alan B Tuck
    Department of Pathology, London Health Sciences Centre, University of Western Ontario, London, Ontario, Canada
    Oncogene 22:1198-205. 2003
    ..A number of potential targets are thus provided for strategies aimed at blocking the malignancy-promoting effects of OPN...
  72. ncbi request reprint Estrous cycle influences organ-specific metastasis of B16F10 melanoma cells
    Sharon A Vantyghem
    Department of Pathology, University of Western Ontario, and London Regional Cancer Centre, London, Ontario, Canada
    Cancer Res 63:4763-5. 2003
    ..We report here that the estrous phase at the time of entry of B16F10 melanoma cells into the circulation leads to marked differences in organ-specific metastasis, suggesting that this concept merits additional study...
  73. ncbi request reprint Activated ras regulates the proliferation/apoptosis balance and early survival of developing micrometastases
    Hemanth J Varghese
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, N6A 5C1 Canada
    Cancer Res 62:887-91. 2002
    ....
  74. ncbi request reprint Plasma osteopontin: associations with survival and metastasis to bone in men with hormone-refractory prostate carcinoma
    Sebastien J Hotte
    Department of Oncology, University of Western Ontario, London Regional Cancer Center, 790 Commmissioners Road East, London, Ontario, Canada N6A 4L6
    Cancer 95:506-12. 2002
    ..Plasma OPN levels have been associated with tumor burden and survival in patients with metastatic breast carcinoma. The authors explored these associations in men with hormone-refractory prostate carcinoma (HRPC)...
  75. ncbi request reprint Cancer spread and micrometastasis development: quantitative approaches for in vivo models
    Ian C MacDonald
    Department of Medical Biophysics, University of Western Ontario, London, Canada
    Bioessays 24:885-93. 2002
    ..Furthermore, solitary tumor cells may remain dormant for long periods of time in secondary sites. These findings suggest that metastatic growth and angiogenesis are prime targets for anti-metastatic therapy...
  76. ncbi request reprint Dissemination and growth of cancer cells in metastatic sites
    Ann F Chambers
    London Regional Cancer Centre, London, Ontario N6A 4L6, Canada
    Nat Rev Cancer 2:563-72. 2002
    ..Inhibition of the growth of metastases in secondary sites offers a promising approach for cancer therapy...
  77. ncbi request reprint Persistence of solitary mammary carcinoma cells in a secondary site: a possible contributor to dormancy
    George N Naumov
    Department of Medical Biophysics, University of Western Ontario, London, Ontario, N6A 5C1 Canada
    Cancer Res 62:2162-8. 2002
    ..If such cells are present in patients, they could contribute to tumor recurrence and would not be susceptible to current therapeutic strategies targeting proliferating cells...
  78. ncbi request reprint Osteopontin identified as lead marker of colon cancer progression, using pooled sample expression profiling
    Deepak Agrawal
    Department of Cell Biology, Interdisciplinary Oncology, H Lee Moffitt Cancer Center, University of South Florida, Tampa 33612, USA
    J Natl Cancer Inst 94:513-21. 2002
    ..Gene expression profiling techniques offer the opportunity to discover such markers. We investigated the feasibility of sample pooling strategy in combination with a novel analysis algorithm to identify markers...
  79. ncbi request reprint Correlation of osteopontin protein expression and pathological stage across a wide variety of tumor histologies
    Domenico Coppola
    Department of Pathology, University of South Florida College of Medicine, Tampa, Florida, USA
    Clin Cancer Res 10:184-90. 2004
    ..Although various studies have assessed OPN protein levels in several tumor types, a broad survey of OPN expression in human neoplasia under the same experimental conditions has not been carried out...
  80. doi request reprint New clinical and experimental approaches for studying tumor dormancy: does tumor dormancy offer a therapeutic target?
    Paul Goss
    Massachusetts General Hospital Cancer Center, and Department of Medicine, Harvard Medical School, Boston, MA, USA
    APMIS 116:552-68. 2008
    ..A better understanding of mechanisms responsible for tumor dormancy and recurrence will be important for improving care of patients at risk for late-developing metastases...
  81. ncbi request reprint Enhanced cell surface CD44 variant (v6, v9) expression by osteopontin in breast cancer epithelial cells facilitates tumor cell migration: novel post-transcriptional, post-translational regulation
    Subarna A Khan
    Department of Cell Biology and Neuroscience, Nelson Laboratories, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA
    Clin Exp Metastasis 22:663-73. 2005
    ..Our data provide evidence that in 21NT cells OPN enhances CD44s mRNA expression, increases cell surface expression of CD44 variant forms without a change in mRNA levels, and stimulates cell migration...
  82. pmc Inhibition of metastatic outgrowth from single dormant tumor cells by targeting the cytoskeleton
    Dalit Barkan
    Laboratory of Cell Biology and Genetics, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 68:6241-50. 2008
    ..Targeting this process may provide therapeutic strategies for inhibition of the dormant-to-proliferative metastatic switch...
  83. ncbi request reprint Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences
    Shigeyuki Kon
    Division of Molecular Immunology, Institute for Genetic Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo, 060 0815, Japan
    J Cell Biochem 84:420-32. 2002
    ..The two distinct epitopes defined by 2K1 and mAb53 antibodies are closely related to the SVVYGLR cell-binding domain and the GLRSKS containing thrombin cleavage site, respectively, and are involved in cell binding and cell migration...
  84. ncbi request reprint Osteopontin identified as colon cancer tumor progression marker
    Deepak Agrawal
    Department of Cell Biology, H Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA
    C R Biol 326:1041-3. 2003
    ..Osteopontin is a secreted glycoprotein known to bind integrins and CD44. Its actual molecular function remains elusive but its increased expression correlates strongly with tumor progression...
  85. pmc Osteopontin knockdown suppresses tumorigenicity of human metastatic breast carcinoma, MDA-MB-435
    Lalita A Shevde
    University of South Alabama Mitchell Cancer Institute, Mobile, AL 36688 0002, USA
    Clin Exp Metastasis 23:123-33. 2006
    ..Thus, in addition to the widely reported roles of OPN in late stages of tumor progression, these results provide functional evidence that OPN contributes to breast tumor growth as well...
  86. ncbi request reprint Brain metastases of breast cancer
    Diane Palmieri
    Women s Cancers Section, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
    Breast Dis 26:139-47. 2006
    ..New therapies with permeability for the blood-brain barrier are needed to counteract both types of tumor cells...
  87. ncbi request reprint The biology of metastasis to a sanctuary site
    Diane Palmieri
    Women s Cancers Section, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Clin Cancer Res 13:1656-62. 2007
    ..Specific, new approaches to combat brain metastatic disease are needed...
  88. pmc Osteopontin expression in normal skin and non-melanoma skin tumors
    Pi Ling Chang
    Department of Nutrition Sciences, 311 Susan Mott Webb Nutrition Sciences Building, 1675 University Boulevard, University of Alabama at Birmingham, Birmingham, Alabama 35295 3360, USA
    J Histochem Cytochem 56:57-66. 2008
    ..In conclusion, these data suggest that OPN is associated with keratinocyte differentiation and that it is expressed in AK and SCC, which have metastatic potential, but minimally expressed in solid BCC...
  89. ncbi request reprint Serial plasma osteopontin levels have prognostic value in metastatic breast cancer
    Vivien H C Bramwell
    Tom Baker Cancer Centre, Calgary, Alberta, Canada
    Clin Cancer Res 12:3337-43. 2006
    ..To evaluate its prognostic value in advanced disease, we conducted a prospective clinical study measuring serial osteopontin plasma levels in women with metastatic breast cancer throughout the course of their disease...
  90. ncbi request reprint Re: Medroxyprogesterone acetate and metastases: of mice and (wo)men
    Ann F Chambers
    J Natl Cancer Inst 97:1225; discussion 1225-6. 2005
  91. ncbi request reprint Transcriptional regulation of osteopontin and the metastatic phenotype: evidence for a Ras-activated enhancer in the human OPN promoter
    David T Denhardt
    Nelson Laboratories, Rutgers University, Piscataway, New Jersey 88854, USA
    Clin Exp Metastasis 20:77-84. 2003
    ..In this communication we address two issues: (1) How does OPN facilitate the metastatic phenotype? (2) What mechanisms are responsible for the increase in OPN transcription in metastatic cells?..
  92. ncbi request reprint Osteopontin and colon cancer progression
    Timothy J Yeatman
    H Lee Moffitt Cancer Center, Tampa, Florida 33612, USA
    Clin Exp Metastasis 20:85-90. 2003
    ....
  93. ncbi request reprint Osteopontin (Eta-1) and fibroblast growth factor-2 cross-talk in angiogenesis
    Daria Leali
    Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    J Immunol 171:1085-93. 2003
    ..OPN-mediated recruitment of proangiogenic monocytes may represent a mechanism of amplification of FGF2-induced neovascularization during inflammation, wound healing, and tumor growth...
  94. ncbi request reprint Evaluating the role of serine protease inhibition in the management of tumor micrometastases
    Joe B Putnam
    University of Texas, M D Anderson Cancer Center, Houston, Texas, USA
    Oncology (Williston Park) 17:9-30; quiz 31-2. 2003