R B Campenot

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. doi request reprint Production of compartmented cultures of rat sympathetic neurons
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    Nat Protoc 4:1869-87. 2009
  2. doi request reprint NGF uptake and retrograde signaling mechanisms in sympathetic neurons in compartmented cultures
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Results Probl Cell Differ 48:141-58. 2009
  3. ncbi request reprint Protein synthesis in axons and its possible functions
    R B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Neurocytol 29:793-8. 2000
  4. ncbi request reprint Retrograde transport of neurotrophins: fact and function
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    J Neurobiol 58:217-29. 2004
  5. ncbi request reprint Block of slow axonal transport and axonal growth by brefeldin A in compartmented cultures of rat sympathetic neurons
    R B Campenot
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Neuropharmacology 44:1107-17. 2003
  6. ncbi request reprint Ceramide inhibits axonal growth and nerve growth factor uptake without compromising the viability of sympathetic neurons
    E P de Chaves
    Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 276:36207-14. 2001
  7. ncbi request reprint Compartmentalization of choline and acetylcholine metabolism in cultured sympathetic neurons
    M Bussiere
    Canadian Institutes of Health Research Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
    J Biochem 130:561-8. 2001
  8. ncbi request reprint The synthesis and transport of lipids for axonal growth and nerve regeneration
    J E Vance
    Department of Medicine, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 1486:84-96. 2000
  9. ncbi request reprint Spatial requirements for TrkA kinase activity in the support of neuronal survival and axon growth in rat sympathetic neurons
    Bronwyn L MacInnis
    Department of Cell Biology, University of Alberta, 5 14 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Neuropharmacology 45:995-1010. 2003
  10. doi request reprint A retrograde apoptotic signal originating in NGF-deprived distal axons of rat sympathetic neurons in compartmented cultures
    Sue Ann Mok
    Department of Cell Biology, University of Alberta, 5 14 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Cell Res 19:546-60. 2009

Collaborators

  • J E Vance
  • D E Vance
  • Lisa McKerracher
  • Barbara Karten
  • Hideki Hayashi
  • Bronwyn L MacInnis
  • Jodi M Carter
  • Sue Ann Mok
  • Sue A Mok
  • M Bussiere
  • Johanne Bertrand
  • E P de Chaves
  • E I Posse De Chaves
  • Karen Lund
  • Laurent Demizieux
  • Matthew J Winton
  • Nieves Rodriguez-Hernandez
  • Robert A Maue
  • Donna L Senger
  • Kristin A Waite
  • B MacInnis
  • R S Kiss

Detail Information

Publications24

  1. doi request reprint Production of compartmented cultures of rat sympathetic neurons
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    Nat Protoc 4:1869-87. 2009
    ..Here we focus on sympathetic neurons cultured from neonatal rats and provide protocols for the production and some of the uses of compartmented cultures...
  2. doi request reprint NGF uptake and retrograde signaling mechanisms in sympathetic neurons in compartmented cultures
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Results Probl Cell Differ 48:141-58. 2009
    ....
  3. ncbi request reprint Protein synthesis in axons and its possible functions
    R B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Neurocytol 29:793-8. 2000
    ..It remains unclear whether local protein synthesis could supply the cytoskeletal proteins and other slow-transported proteins required for the maintenance, plasticity, and regeneration of long axons...
  4. ncbi request reprint Retrograde transport of neurotrophins: fact and function
    Robert B Campenot
    Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    J Neurobiol 58:217-29. 2004
    ..The newly discovered potential for redundancy in retrograde signaling mechanisms can complicate the interpretation of experimental results...
  5. ncbi request reprint Block of slow axonal transport and axonal growth by brefeldin A in compartmented cultures of rat sympathetic neurons
    R B Campenot
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Neuropharmacology 44:1107-17. 2003
    ....
  6. ncbi request reprint Ceramide inhibits axonal growth and nerve growth factor uptake without compromising the viability of sympathetic neurons
    E P de Chaves
    Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 276:36207-14. 2001
    ..However, inhibition of endocytosis of NGF by ceramide could not be ascribed to decreased phosphorylation of TrkA...
  7. ncbi request reprint Compartmentalization of choline and acetylcholine metabolism in cultured sympathetic neurons
    M Bussiere
    Canadian Institutes of Health Research Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
    J Biochem 130:561-8. 2001
    ..These results suggest that the majority of axonal acetylcholine is synthesized in distal axons/axon terminals from choline taken up by a high-affinity choline transporter in distal axons...
  8. ncbi request reprint The synthesis and transport of lipids for axonal growth and nerve regeneration
    J E Vance
    Department of Medicine, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 1486:84-96. 2000
    ..Lipids are also transported from axons to myelin, although the importance of this process for myelination is not clear...
  9. ncbi request reprint Spatial requirements for TrkA kinase activity in the support of neuronal survival and axon growth in rat sympathetic neurons
    Bronwyn L MacInnis
    Department of Cell Biology, University of Alberta, 5 14 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Neuropharmacology 45:995-1010. 2003
    ..These results support the hypothesis that retrograde signals can be carried by mechanisms downstream of TrkA activity...
  10. doi request reprint A retrograde apoptotic signal originating in NGF-deprived distal axons of rat sympathetic neurons in compartmented cultures
    Sue Ann Mok
    Department of Cell Biology, University of Alberta, 5 14 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7
    Cell Res 19:546-60. 2009
    ..The existence of a retrograde death signaling system in axons that is suppressed by neurotrophins has broad implications for neurodevelopment and for discovering treatments for neurodegenerative diseases and neurotrauma...
  11. ncbi request reprint Retrograde support of neuronal survival without retrograde transport of nerve growth factor
    Bronwyn L MacInnis
    Department of Cell Biology, 6 14 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7
    Science 295:1536-9. 2002
    ..Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it...
  12. ncbi request reprint A nerve growth factor-induced retrograde survival signal mediated by mechanisms downstream of TrkA
    Sue A Mok
    Department of Cell Biology, University of Alberta, 5 14 Medical Sciences Building, Edmonton, Alberta T6G 2H7, Canada
    Neuropharmacology 52:270-8. 2007
    ..The latter effect suggests that downstream retrograde survival signals can arise from alterations in one or more kinase activities in the distal axons without activation of TrkA by NGF...
  13. ncbi request reprint Uptake of lipoproteins for axonal growth of sympathetic neurons
    E I Posse De Chaves
    Departments of Biochemistry, Cell Biology, and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 275:19883-90. 2000
    ..These studies demonstrate for the first time that lipids are taken up from lipoproteins by sympathetic neurons for use in axonal regeneration...
  14. ncbi request reprint Neuronal models for studying lipid metabolism and transport
    Barbara Karten
    Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alta, Canada
    Methods 36:117-28. 2005
    ....
  15. ncbi request reprint Cholesterol accumulates in cell bodies, but is decreased in distal axons, of Niemann-Pick C1-deficient neurons
    Barbara Karten
    Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids and Departments of Medicine, Biochemistry and Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    J Neurochem 83:1154-63. 2002
    ..The altered cholesterol distribution between cell bodies and axons suggests that transport of cholesterol, particularly that synthesized endogenously, from cell bodies to distal axons is impaired in NPC1-deficient neurons...
  16. ncbi request reprint Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching
    Jodi M Carter
    Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids, and Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    J Biol Chem 283:202-12. 2008
    ..These results highlight the importance of CTbeta2 in neurons for promoting neurite outgrowth and branching and represent the first identification of a lipid biosynthetic enzyme that facilitates these functions...
  17. ncbi request reprint Regulation of Wallerian degeneration and nerve growth factor withdrawal-induced pruning of axons of sympathetic neurons by the proteasome and the MEK/Erk pathway
    Bronwyn L MacInnis
    Department of Cell Biology, University of Alberta, 6 22 Medical Sciences Building, Edmonton, AB, Canada T6G 2H7
    Mol Cell Neurosci 28:430-9. 2005
    ..Identification of the pathways that regulate axonal survival and degeneration will provide possible target sites for pharmacological treatments of neurodegenerative diseases and traumatic injury...
  18. ncbi request reprint Trafficking of cholesterol from cell bodies to distal axons in Niemann Pick C1-deficient neurons
    Barbara Karten
    Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 278:4168-75. 2003
    ....
  19. ncbi request reprint Enhanced expression and activation of CTP:phosphocholine cytidylyltransferase beta2 during neurite outgrowth
    Jodi M Carter
    Canadian Institutes of Health Research Group on the Molecular and Cell Biology of Lipids, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 278:44988-94. 2003
    ..Together, these data suggest that the CTbeta2 isoform of CT is specifically up-regulated and activated during neuronal differentiation to increase PC biosynthesis for growing neurites...
  20. ncbi request reprint The Niemann-Pick C1 protein in recycling endosomes of presynaptic nerve terminals
    Barbara Karten
    Canadian Institutes for Health Research Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, Canada
    J Lipid Res 47:504-14. 2006
    ..We propose that NPC1 plays a previously unrecognized role in the presynaptic nerve terminal and that NPC1 deficiency at this site might contribute to the progressive neurological impairment in NPC disease...
  21. ncbi request reprint Methods for the study of lipid metabolism in neurons
    Hideki Hayashi
    Group on Molecualr and Cell Biology of Lipids and Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    Anal Biochem 331:1-16. 2004
  22. ncbi request reprint Glial lipoproteins stimulate axon growth of central nervous system neurons in compartmented cultures
    Hideki Hayashi
    Department of Medicine, Canadian Institutes of Health Research Group on Molecular and Cell Biology of Lipids, University of Alberta, Alberta, Canada
    J Biol Chem 279:14009-15. 2004
    ..These observations suggest that glial cells stimulate axon growth of CNS neurons by providing lipoproteins containing cholesterol and apolipoprotein E to distal axons...
  23. ncbi request reprint Apolipoprotein E-containing lipoproteins protect neurons from apoptosis via a signaling pathway involving low-density lipoprotein receptor-related protein-1
    Hideki Hayashi
    Group on Molecular and Cell Biology of Lipids and Department of Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    J Neurosci 27:1933-41. 2007
    ..These findings indicate the potential for using glial lipoproteins or an activator of the LRP signaling pathway for treatment for neurodegenerative disorders such as Alzheimer's disease...
  24. ncbi request reprint Application of Rho antagonist to neuronal cell bodies promotes neurite growth in compartmented cultures and regeneration of retinal ganglion cell axons in the optic nerve of adult rats
    Johanne Bertrand
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Montreal, Quebec, Canada, H3T 1J4
    J Neurosci 25:1113-21. 2005
    ..Together, our results indicate that application of a Rho antagonist at the cell body is neuroprotective and overcomes growth inhibition but does not fully prime RGCs for active growth...