Michael Butler

Summary

Affiliation: University of Manitoba
Country: Canada

Publications

  1. ncbi Application of a serum-free medium for the growth of Vero cells and the production of reovirus
    M Butler
    Departments of Microbiology and Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Biotechnol Prog 16:854-8. 2000
  2. ncbi Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals
    Michael Butler
    Department of Microbiology, University of Manitoba, Buller Building, Winnipeg, Canada
    Appl Microbiol Biotechnol 68:283-91. 2005
  3. doi Recent advances in technology supporting biopharmaceutical production from mammalian cells
    M Butler
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Appl Microbiol Biotechnol 96:885-94. 2012
  4. pmc Optimisation of the cellular metabolism of glycosylation for recombinant proteins produced by Mammalian cell systems
    M Butler
    Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada
    Cytotechnology 50:57-76. 2006
  5. doi CHO cells adapted to hypothermic growth produce high yields of recombinant beta-interferon
    Kevin Sunley
    Dept of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Biotechnol Prog 24:898-906. 2008
  6. ncbi Application of the StrOligo algorithm for the automated structure assignment of complex N-linked glycans from glycoproteins using tandem mass spectrometry
    Martin Ethier
    Department of Chemistry, University of Manitoba, Winnipeg, MB, Canada
    Rapid Commun Mass Spectrom 17:2713-20. 2003
  7. doi High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions
    Tharmala Tharmalingam
    Dept of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2
    Biotechnol Prog 24:832-8. 2008
  8. ncbi The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells
    Veronica Restelli
    Department of Microbiology, University of Manitoba, 118 Buller Bldg, Winnipeg, Manitoba R3T 2N2, Canada
    Biotechnol Bioeng 94:481-94. 2006
  9. ncbi Production and glycosylation of recombinant beta-interferon in suspension and cytopore microcarrier cultures of CHO cells
    Maureen Spearman
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Biotechnol Prog 21:31-9. 2005
  10. doi The changing dielectric properties of CHO cells can be used to determine early apoptotic events in a bioprocess
    Katrin Braasch
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Biotechnol Bioeng 110:2902-14. 2013

Collaborators

  • Bo Liu
  • Jeremy P Kunkel
  • Jaak C Jaeken
  • Suh Chin Wu
  • Jose Rodriguez
  • Oleg V Krokhin
  • G Matthijs
  • Kevin Sunley
  • Maureen Spearman
  • Chia Chyi Liu
  • Tharmala Tharmalingam
  • Katrin Braasch
  • Martin Ethier
  • Helene Perreault
  • Julian A Saba
  • Veronica Restelli
  • Kenneth G Standing
  • Carly Lodewyks
  • Meika Richmond
  • Elham Salimi
  • Greg E Bridges
  • Doug J Thomson
  • Tim Cabel
  • Marija Nikolic-Jaric
  • Shiang Chi Lee
  • Wei Cheng Lian
  • Norman Huzel
  • Ming dong Wang
  • Norm Huzel
  • Werner Ens
  • Werner E Ens
  • David C H Jan
  • James C Jamieson

Detail Information

Publications18

  1. ncbi Application of a serum-free medium for the growth of Vero cells and the production of reovirus
    M Butler
    Departments of Microbiology and Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Biotechnol Prog 16:854-8. 2000
    ..The high viral productivity suggests that this is a suitable system for the production of reovirus as an oncolytic agent for human therapeutic use...
  2. ncbi Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals
    Michael Butler
    Department of Microbiology, University of Manitoba, Buller Building, Winnipeg, Canada
    Appl Microbiol Biotechnol 68:283-91. 2005
    ..However, some analysts indicate that this still may not be enough and that future manufacturing demand may exceed production capacity as the number of approved biotherapeutics increases...
  3. doi Recent advances in technology supporting biopharmaceutical production from mammalian cells
    M Butler
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Appl Microbiol Biotechnol 96:885-94. 2012
    ..Finally, advances in downstream processing are needed to handle the increased supply of product from the bioreactor but maintaining the high purity demanded of these biopharmaceuticals...
  4. pmc Optimisation of the cellular metabolism of glycosylation for recombinant proteins produced by Mammalian cell systems
    M Butler
    Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada
    Cytotechnology 50:57-76. 2006
    ....
  5. doi CHO cells adapted to hypothermic growth produce high yields of recombinant beta-interferon
    Kevin Sunley
    Dept of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
    Biotechnol Prog 24:898-906. 2008
    ..This eventually resulted in a threefold enhancement of volumetric titer of monomeric beta-interferon compared to the original control culture at 37 degrees C...
  6. ncbi Application of the StrOligo algorithm for the automated structure assignment of complex N-linked glycans from glycoproteins using tandem mass spectrometry
    Martin Ethier
    Department of Chemistry, University of Manitoba, Winnipeg, MB, Canada
    Rapid Commun Mass Spectrom 17:2713-20. 2003
    ..The analysis was completed in less than 2 minutes for any glycan, which is a substantial improvement over manual interpretation...
  7. doi High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions
    Tharmala Tharmalingam
    Dept of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2
    Biotechnol Prog 24:832-8. 2008
    ..These results indicate that the hypothermic conditions in a Cytopore culture had a combined and possibly synergistic effect of increasing volumetric production of the recombinant protein...
  8. ncbi The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells
    Veronica Restelli
    Department of Microbiology, University of Manitoba, 118 Buller Bldg, Winnipeg, Manitoba R3T 2N2, Canada
    Biotechnol Bioeng 94:481-94. 2006
    ..This observation of lower fucosylation at high or low DO levels is consistent with previous data reported for glycoprotein production in insect cells...
  9. ncbi Production and glycosylation of recombinant beta-interferon in suspension and cytopore microcarrier cultures of CHO cells
    Maureen Spearman
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Biotechnol Prog 21:31-9. 2005
    ..This suggests that Cytopore microcarriers may be useful in bioprocess development for enhanced recombinant glycoprotein production without affecting the glycosylation profile of the protein...
  10. doi The changing dielectric properties of CHO cells can be used to determine early apoptotic events in a bioprocess
    Katrin Braasch
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
    Biotechnol Bioeng 110:2902-14. 2013
    ....
  11. doi Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest
    Kevin Sunley
    Department of Microbiology, University of Manitoba, Winnipeg, Canada
    Biotechnol Adv 28:385-94. 2010
    ..This review highlights the shared biology of these three controlled proliferation strategies, and their contribution towards bioprocess development for recombinant production from animal cell cultures...
  12. doi Monitoring cell growth, viability, and apoptosis
    Michael Butler
    Animal Cell Technology, Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada
    Methods Mol Biol 1104:169-92. 2014
    ..Alternatively, radioactive techniques may be used for an accurate determination of cellular protein synthesis...
  13. ncbi The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody
    Bo Liu
    Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
    J Biotechnol 170:17-27. 2014
    ..The high GI of the chimeric antibody (EG2) may be due to its low molecular weight and unusual structure. These findings are important in relation to the low substrate that may occur in fed-batch cultures for Mab production. ..
  14. ncbi The bioactivity and fractionation of peptide hydrolysates in cultures of CHO cells
    Maureen Spearman
    Dept of Microbiology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada
    Biotechnol Prog 30:584-93. 2014
    ..2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:584-593, 2014. ..
  15. ncbi A study of immunoglobulin G glycosylation in monoclonal and polyclonal species by electrospray and matrix-assisted laser desorption/ionization mass spectrometry
    Julian A Saba
    Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba R3T 2N2, Canada
    Anal Biochem 305:16-31. 2002
    ..In contrast to FACE and HPAEC-PAD, MALDI-double quadrupole/time-of-flight MS and HPLC/ESI-MS also detected low-abundance high-mannose and hybrid structures in some of the species under investigation...
  16. ncbi Detailed glycan analysis of serum glycoproteins of patients with congenital disorders of glycosylation indicates the specific defective glycan processing step and provides an insight into pathogenesis
    Michael Butler
    The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford, OX1 3QU, UK
    Glycobiology 13:601-22. 2003
    ....
  17. pmc High genetic stability of dengue virus propagated in MRC-5 cells as compared to the virus propagated in vero cells
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    PLoS ONE 3:e1810. 2008
    ..Serum-free microcarrier cultures of MRC-5 cells offer a potentially valuable system for the large-scale production of live-attenuated DEN vaccines...
  18. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines...