Genomes and Genes
Affiliation: University of Toronto
- Subretinal gene delivery using helper-dependent adenoviral vectorsLinda Wu
Physiology and Experimental Medicine Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G1X8, Canada
Cell Biosci 1:15. 2011..Since these vectors have a large cloning capacity, they have great potential to extend the success of gene therapy achieved using the adeno-associated viral vector...
- Retinoblastoma, an inside jobRod Bremner
Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada
Cell 137:992-4. 2009..Why are some cell types more prone to transformation than others? In this issue, Xu et al. (2009) show that retinoblastoma cells co-opt several intrinsic features of cone photoreceptors for their survival and growth...
- The RB protein family in retinal development and retinoblastoma: new insights from new mouse modelsRod Bremner
Toronto Western Research Institute, University Health Network, Rm Mc6 424, 399 Bathurst Street, University of Toronto, Toronto, Ontario M5T 2S8, Canada
Dev Neurosci 26:417-34. 2004..The new mouse models of retinoblastoma will be invaluable for testing these possibilities...
- Cyclins, Cdks, E2f, Skp2, and more at the first international RB Tumor Suppressor MeetingRod Bremner
Toronto Western Research Institute, Toronto, Ontario, Canada
Cancer Res 70:6114-8. 2010..We summarize major new breakthroughs and emerging trends presented at the meeting...
- Division and apoptosis of E2f-deficient retinal progenitorsDanian Chen
Toronto Western Research Institute, University Health Network, Department of Ophthalmology, and Laboratory Medicine and Pathobiology, University of Toronto, Ontario M5T 2S8, Canada
Nature 462:925-9. 2009..Thus, activating E2fs are not universally required for normal mammalian cell division, but have an unexpected pro-survival role in development...
- The chromatin-remodeling enzyme BRG1 coordinates CIITA induction through many interdependent distal enhancersZuyao Ni
Genetics and Development Division, Toronto Western Research Institute, University Health Network, Toronto, Ontario M5T 2S8, Canada
Nat Immunol 9:785-93. 2008..Like BRG1, each remote element was critical for the induction of CIITA expression. Thus, BRG1 regulates CIITA through many interdependent remote enhancers, not through the promoter alone...
- Chx10 is required to block photoreceptor differentiation but is dispensable for progenitor proliferation in the postnatal retinaIzzy Livne-Bar
Toronto Western Research Institute, University Health Network
Proc Natl Acad Sci U S A 103:4988-93. 2006..Thus, Chx10 directly controls bipolar cell genesis by inhibiting rod differentiation independent of its temporally limited early effect on RPC proliferation...
- CHX10 targets a subset of photoreceptor genesKimberley M Dorval
Toronto Western Research Institute, University Health Network, Ontario, Canada
J Biol Chem 281:744-51. 2006..Our data suggest that CHX10 may target these motifs to inhibit rod photoreceptor gene expression in bipolar cells...
- Rb-mediated neuronal differentiation through cell-cycle-independent regulation of E2f3aDanian Chen
Genetics and Development Division, Toronto Western Research Institute, University Health Network, University of Toronto, Ontario, Canada
PLoS Biol 5:e179. 2007..These data reveal a mechanism through which Rb regulates neural differentiation directly, and, unexpectedly, it involves inhibition of E2f3a, not potentiation of tissue-specific factors...
- Maximizing functional photoreceptor differentiation from adult human retinal stem cellsTomoyuki Inoue
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
Stem Cells 28:489-500. 2010..This study suggests that gene modulation in human RSCs may provide a source of photoreceptor cells for the treatment of photoreceptor disease...
- Brahma-related gene 1-dependent STAT3 recruitment at IL-6-inducible genesZuyao Ni
Toronto Western Research Institute, University Health Network, Department of Ophthalmology and Vision Science, Vision Science Research Program, University of Toronto, Ontario, Canada
J Immunol 178:345-51. 2007....
- Transcriptional activity of the paired-like homeodomain proteins CHX10 and VSX1Kimberley M Dorval
Toronto Western Research Institute, University Health Network Program, University of Toronto, Toronto, Ontario M5T 2S8, Canada
J Biol Chem 280:10100-8. 2005..Therefore, CHX10 and VSX1 may control retinal bipolar cell specification or differentiation by repressing genes required for the development of other cell types...
- Cell-specific effects of RB or RB/p107 loss on retinal development implicate an intrinsically death-resistant cell-of-origin in retinoblastomaDanian Chen
Toronto Western Research Institute, University Health Network, Vision Science Research Program, Department of Ophthalmology and Visual Sciences, University of Toronto, 399 Bathurst Street, Toronto, Ontario, Canada, M5T 2S8
Cancer Cell 5:539-51. 2004..We suggest that additional lesions common in retinoblastoma overcome growth arrest, not apoptosis...
- Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neuronsMarek Pacal
Genetics and Development Division, Toronto Western Research Institute, University Health Network, Toronto, Canada
Dev Dyn 241:1525-44. 2012..Here, we timed cell cycle and neuronal protein silencing/induction during cell birth in the developing murine retina...
- Retinoblastoma protein purification and transduction of retina and retinoblastoma cells using improved alphavirus vectorsDavid P Diciommo
Ontario Cancer Institute Cancer Informatics, University Health Network, Toronto, Canada
Invest Ophthalmol Vis Sci 45:3320-9. 2004..To develop and use improved Semliki Forest vectors (SFVs) for functional and structural analyses of the retinoblastoma protein (RB) in developing retina and retinoblastoma cells...
- A G1 checkpoint mediated by the retinoblastoma protein that is dispensable in terminal differentiation but essential for senescenceSrikanth Talluri
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
Mol Cell Biol 30:948-60. 2010..Remarkably, this reveals that pRB recruits chromatin regulators primarily to engage a stress-responsive G(1) arrest program...
- The double-stranded RNA-binding protein Staufen 2 regulates eye sizeDiane M Cockburn
Toronto Western Research Institute, Genetics and Development Division, MCL 6 415, Toronto M5T 2S8, Ontario, Canada
Mol Cell Neurosci 51:101-11. 2012..These data highlight a new biological function for Stau2 and suggest that translation control of specific Stau2-associated transcripts may be a key regulator of tissue size...
- Insights from animal models on the origins and progression of retinoblastomaMarek Pacal
Toronto Western Research Institute, University Health Network, 399 Bathurst Street, Room Mc6 424, Toronto, Ontario, M5T 2S8 Canada
Curr Mol Med 6:759-81. 2006..Two years have dramatically improved our understanding of Rb function in the tissue that gave us this important tumor suppressor...
- Direct and indirect effects of hedgehog pathway activation in the mammalian retinaChuan Yu
Molecular Medicine Program, Ottawa Health Research Institute and University of Ottawa Eye Institute, Ottawa, Ontario, Canada
Mol Cell Neurosci 32:274-82. 2006..SMO-M2 expression also inhibited rhodopsin expression in uninfected cells, thus highlighting an unexpected non-cell autonomous effect of Hh pathway activation on photoreceptor development...
- Apical role for BRG1 in cytokine-induced promoter assemblyZuyao Ni
Toronto Western Research Institute, University Health Network, Department of Ophthalmology and Vision Science, Vision Science Research Program, University of Toronto, Toronto, ON, Canada M5T 2S8
Proc Natl Acad Sci U S A 102:14611-6. 2005..Furthermore, BRG1 is required for IFN-induced restriction enzyme and DNase I accessibility at promoters. Thus, BRG1 has an apical role in cytokine-induced promoter assembly, acting upstream of STAT complexes at multiple IFN target loci...
- VSX1: a gene for posterior polymorphous dystrophy and keratoconusElise Heon
Cellular and Molecular Division, Toronto Western Research Institute, Toronto Western Hospital, 399 Bathurst Street, Toronto, Ontario, Canada M5T 2S8
Hum Mol Genet 11:1029-36. 2002..These data define the molecular basis of two important corneal dystrophies and reveal the importance of the CVC domain in the human retina...
- Association of reading disabilities with regions marked by acetylated H3 histones in KIAA0319Jillian M Couto
Genetics and Development Division, Toronto Western Research Institute, Toronto Western Hospital, University Health Network, Toronto, Ontario, Canada
Am J Med Genet B Neuropsychiatr Genet 153:447-62. 2010..Our data indicates that this putative regulatory region is a likely site of genetic variation contributing to RD in our sample, further narrowing the candidate region...
- The search for the retinoblastoma cell of originMichael A Dyer
Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Nat Rev Cancer 5:91-101. 2005..Identifying the cell of origin for this tumour would advance our understanding of how cellular context affects the requirement of specific mutations for cancer initiation and progression...
- Murine cytomegalovirus paralyzes macrophages by blocking IFN gamma-induced promoter assemblyDaniel L Popkin
Department of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 100:14309-14. 2003....
- Re: Update on retinoblastomaRod Bremner
Retina 25:950-1; author reply 951-2. 2005
- Unique requirement for Rb/E2F3 in neuronal migration: evidence for cell cycle-independent functionsKelly A McClellan
Department of Cellular and Molecular Medicine, Ottawa Health Research Institute, University of Ottawa, Ottawa, ON, Canada
Mol Cell Biol 27:4825-43. 2007..This study not only outlines overlapping and distinct functions for E2Fs in neurogenesis but also is the first to establish a physiologically relevant role for the Rb/E2F pathway beyond cell cycle regulation in vivo...