Research Topics
Species | J AusioSummaryAffiliation: University of Victoria Country: Canada Publications
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Publications
Vertebrate nucleoplasmin and NASP: egg histone storage proteins with multiple chaperone activitiesRon M Finn
2Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 258a, Victoria, BC, Canada V8W 3P6
FASEB J 26:4788-804. 2012..Finn, R. M., Ellard, K., Eirín-López, J. M., Ausió, J. Vertebrate nucleoplasmin and NASP: egg histone storage proteins with multiple chaperone activities...
A unified phylogeny-based nomenclature for histone variantsPaul B Talbert
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA
Epigenetics Chromatin 5:7. 2012..For clarity and searchability, we encourage the use of descriptors that are separate from the phylogeny-based variant name to indicate developmental and other properties of variants that may be independent of structure...
The characterization of amphibian nucleoplasmins yields new insight into their role in sperm chromatin remodelingLindsay J Frehlick
Department of Biochemistry and Microbiology, University of Victoria, Petch Building, Victoria, BC, V8W 3P6, Canada
BMC Genomics 7:99. 2006....- The evolutionary differentiation of two histone H2A.Z variants in chordates (H2A.Z-1 and H2A.Z-2) is mediated by a stepwise mutation process that affects three amino acid residuesJosé M Eirín-López
Departamento de Biologia Celular y Molecular, Universidade da Coruna, Coruna, Spain
BMC Evol Biol 9:31. 2009..Z generically as a single protein, in vertebrates it is a mixture of two protein forms H2A.Z-1 (previously H2A.Z) and H2A.Z-2 (previously H2A.F/Z or H2A.V) that differ by three amino acids... - Characterization of the histone H2A.Z-1 and H2A.Z-2 isoforms in vertebratesDeanna Dryhurst
Department of Biochemistry and Microbiology and The Center for Biomedical Research, University of Victoria, Petch Building, Victoria, BC, V8W 3P6, Canada
BMC Biol 7:86. 2009..Z mediates its effects are not entirely understood. However, it is now known that H2A.Z has two protein isoforms in vertebrates, H2A.Z-1 and H2A.Z-2, which are encoded by separate genes and differ by 3 amino acid residues... 
Evolution of vertebrate chromosomal sperm proteins: implications for fertility and sperm competitionJuan Ausio
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Soc Reprod Fertil Suppl 65:63-79. 2007..All of this suggests a potential involvement of protamines in sperm competition which is discussed in this paper...- Histone variants--the structure behind the functionJuan Ausio
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
Brief Funct Genomic Proteomic 5:228-43. 2006..Understanding and deciphering the underlying structural amino acid code behind such variability remains one of the most exciting future challenges in chromatin research... - The many tales of a tail: carboxyl-terminal tail heterogeneity specializes histone H2A variants for defined chromatin functionJuan Ausio
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada V8W 3P6
Biochemistry 41:5945-9. 2002.... - Histone variants and histone modifications: a structural perspectiveJ Ausio
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
Biochem Cell Biol 79:693-708. 2001..Recent work on this topic emphasizes an earlier notion that suggests that, in many instances, the functional response to histone variability is possibly the result of a synergistic structural effect... - Syndromes of disordered chromatin remodelingJ Ausio
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Clin Genet 64:83-95. 2003.... - Analytical ultracentrifugation and the characterization of chromatin structureJ Ausio
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
Biophys Chem 86:141-53. 2000..As an example he has devoted some of his research effort to the characterization of chromatin in solution. This review includes these important contributions... - Are linker histones (histone H1) dispensable for survival?J Ausio
Department of Biochemistry and Microbiology, University of Victoria, Petch Building 220, Victoria, British Columbia, Canada V8W 3P6
Bioessays 22:873-7. 2000..These results suggest that while linker histones may be dispensable for the relatively short life span of an individual cell, they are most likely indispensable for survival of higher eukaryote organisms... - The histidine-rich protamine from ostrich and tinamou sperm. A link between reptile and bird protaminesJ Ausio
Department of Biochemistry and Microbiology, University of Victoria, B C, Canada
Biochemistry 38:180-4. 1999..Furthermore, these results shed some additional light on the controversial origin of birds. They provide further molecular support to the fossil record that suggests that reptiles and birds are closely related... - Effects of histone acetylation on the solubility and folding of the chromatin fiberX Wang
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
J Biol Chem 276:12764-8. 2001.... - Long-term evolution and functional diversification in the members of the nucleophosmin/nucleoplasmin family of nuclear chaperonesJosé M Eirín-López
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
Genetics 173:1835-50. 2006.... - Dimerization stabilizes the pore-forming toxin aerolysin in solutionF G van der Goot
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
J Biol Chem 268:18272-9. 1993..These substitutions are known to increase the protein's ability to oligomerize, supporting the conclusion that dimer dissociation is necessary for oligomerization to occur... - Nucleoplasmin-mediated unfolding of chromatin involves the displacement of linker-associated chromatin proteinsIsbaal Ramos
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Biochemistry 44:8274-81. 2005..The results are discussed within the context of the possible implication of nucleoplasmin in processes such as transcription and replication licensing which take place after egg fertilization at the onset of development... - Origin of H1 linker histonesH E Kasinsky
Department of Biochemistry and Microbiology, University of Victoria, Victoria, B C, Canada
FASEB J 15:34-42. 2001.... - Interaction of daunomycin with acetylated chromatinLyndsay Sprigg
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8W 3P6, Canada
J Med Chem 53:6457-65. 2010..Furthermore, the binding of anthracycline to acetylated chromatin sheds additional light into the conformational chromatin alterations resulting from core histone acetylation... - The sperm proteins from amphioxus mirror its basal position among chordates and redefine the origin of vertebrate protaminesJose Maria Eirin-Lopez
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Mol Biol Evol 25:1705-13. 2008..Given that tunicates are now viewed as a sister group of vertebrates, the lysine to arginine transition responsible for the origin of vertebrate protamines must be set a step back from tunicates... - Acetylation of vertebrate H2A.Z and its effect on the structure of the nucleosomeToyotaka Ishibashi
Department of Biochemistry and Microbiology and The Center for Biomedical Research, University of Victoria, Petch Building, Victoria, BC, Canada V8W 3P6
Biochemistry 48:5007-17. 2009..Furthermore, the simultaneous occurrence of H2A.Z and H2A in heteromorphic NCPs that most likely occurs in vivo slightly destabilizes the NCP, but only in the presence of acetylation... - Structural characterization of macroH2A containing chromatinD Wade Abbott
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada, V8W 3P6
Biochemistry 43:1352-9. 2004.... - Common phylogenetic origin of protamine-like (PL) proteins and histone H1: Evidence from bivalve PL genesJosé M Eirín-López
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Mol Biol Evol 23:1304-17. 2006.... - A walk though vertebrate and invertebrate protaminesJohn D Lewis
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
Chromosoma 111:473-82. 2003..The review is based on some of the most recent publications in the field and builds upon previously published reviews on this topic... - Sperm nuclear basic proteins of two closely related species of Scorpaeniform fish (Sebastes maliger, Sebastolobus sp.) with different sexual reproduction and the evolution of fish protaminesLindsay J Frehlick
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada V8W 3P6
J Exp Zoolog A Comp Exp Biol 305:277-87. 2006.... - H2A.Bbd: an X-chromosome-encoded histone involved in mammalian spermiogenesisToyotaka Ishibashi
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 3P6, Canada
Nucleic Acids Res 38:1780-9. 2010..This common rate of evolution provides further support for the functional and structural involvement of this protein in male gametogenesis in mammals... - MeCP2 preferentially binds to methylated linker DNA in the absence of the terminal tail of histone H3 and independently of histone acetylationToyotaka Ishibashi
Department of Biochemistry and Microbiology, The Center for Biomedical Research, University of Victoria, Victoria, BC, Canada V8W 3P6
FEBS Lett 582:1157-62. 2008..However, its binding specificity strongly depends on DNA methylation. We also observed that as with linker histones, this binding is independent of the core histone H3 N-terminal tail and is not affected by histone acetylation... - MBD4-mediated glycosylase activity on a chromatin template is enhanced by acetylationToyotaka Ishibashi
Department of Biochemistry and Microbiology, University of Victoria, P O Box 3055, Victoria, BC, Canada V8W 3P6
Mol Cell Biol 28:4734-44. 2008..However, histone hyperacetylation facilitated the efficiency with which the bases were excised from the nucleosome templates, irrespective of the position of the mismatch relative to the pseudodyad axis of symmetry of the nucleosome... - Characterization of Inhibitor of Growth 2 tumor suppressor in Alligator mississippiensis, its conservation in Archosauria, and response to thyroid stimulating hormoneCaren C Helbing
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
Comp Biochem Physiol B Biochem Mol Biol 146:279-88. 2007..ING2 expression in the thyroid gland, gonad, and heart was unaffected by TSH treatment. These data indicate that control of ING2 expression by the thyroid axis may be conserved among species and is tissue-dependent... - Beyond the Xi: macroH2A chromatin distribution and post-translational modification in an avian systemD Wade Abbott
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
J Biol Chem 280:16437-45. 2005..This report provides new clues into how mH2A distribution and a previously unidentified post-translational modification may help regulate the repression of autosomal chromatin... - Protamine-like proteins: evidence for a novel chromatin structureJohn D Lewis
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
Biochem Cell Biol 80:353-61. 2002..Potential post-translational modifications and the control of PL protein expression and deposition are also discussed... - H2A.Z stabilizes chromatin in a way that is dependent on core histone acetylationAnita A Thambirajah
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
J Biol Chem 281:20036-44. 2006..These studies also showed that the late elution of this variant (correlated to enhanced binding stability) was independent of the chromatin size and of the presence or absence of linker histones... - Histone H1 and the origin of protaminesJohn D Lewis
Department of Biochemistry and Microbiology, University of Victoria, P.O. Box 3055, Petch Building, Victoria, British Columbia, Canada V8W 3P6
Proc Natl Acad Sci U S A 101:4148-52. 2004.... - All roads lead to arginine: the squid protamine geneJohn D Lewis
Department of Biochemistry and Microbiology, University of Victoria, P.O. Box 3055, Petch Building Room 220, Victoria, B.C, V8W 3P6, Canada
J Mol Evol 58:673-80. 2004..It is unclear whether the similarities of protamines in species from both the deuterostome and the protostome branches represent the result of phylogenetic conservation or evolutionary convergence... - Protamine-like sperm nuclear basic proteins in the primitive frog Ascaphus truei and histone reversions among more advanced frogsH E Kasinsky
Department of Zoology, University of British Columbia, Vancouver, Canada
J Exp Zool 284:717-28. 1999..truei introsperm to neobatrachian aquasperm that Jamieson et al. (1993. Herpetologica 49:52-65) attribute as a secondary reversion to external fertilization... - H2A.Bbd: a quickly evolving hypervariable mammalian histone that destabilizes nucleosomes in an acetylation-independent wayJose Maria Eirin-Lopez
Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 258a, Victoria, BC, Canada V8W 3P6
FASEB J 22:316-26. 2008..This suggests that replacement by H2A.Bbd provides an alternative mechanism to unfold chromatin structure, possibly in euchromatic regions, in a way that is not dependent on acetylation... - Characterization of nucleosomes consisting of the human testis/sperm-specific histone H2B variant (hTSH2B)Andra Li
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Biochemistry 44:2529-35. 2005..Furthermore, the presence of hTSH2B in the nucleosome does not affect its ability to bind to linker histones... - Probasin promoter assembles into a strongly positioned nucleosome that permits androgen receptor bindingAllison H Maffey
Department of Biochemistry and Microbiology, University of Victoria, P O Box 3055, Petch Building, Victoria, BC, Canada V8W 3P6
Mol Cell Endocrinol 268:10-9. 2007..Thus the AR-DBD is able to bind to its cognate sequences within the PB promoter in a way that is indifferent to the presence or absence of histones and nucleosomal structure... - Phosphorylation of histone H2A.X by DNA-dependent protein kinase is not affected by core histone acetylation, but it alters nucleosome stability and histone H1 bindingAndra Li
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
J Biol Chem 285:17778-88. 2010..Finally, we show that although H2A.X does not affect nucleosome conformation, it has a de-stabilizing effect that is enhanced by the DNA-PK-mediated phosphorylation and results in an impaired histone H1 binding... - New insights into the nucleophosmin/nucleoplasmin family of nuclear chaperonesLindsay J Frehlick
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
Bioessays 29:49-59. 2007.... - A haploid affair: core histone transitions during spermatogenesisJohn D Lewis
Department of Biochemistry and Microbiology, Unversity of Victoria, BC, Canada
Biochem Cell Biol 81:131-40. 2003..This review will examine recent discoveries concerning the role of histone modifications and variants during meiosis and spermatogenesis... - Long-range histone acetylation: biological significance, structural implications, and mechanismsAlison Calestagne-Morelli
Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 220, Victoria, BC V8W 3P6, Canada
Biochem Cell Biol 84:518-27. 2006..These include the thermal, ionic, cumulative, and isoform-specific (H4 K16) consequences of acetylation that result in a more dynamic core complex and chromatin fiber... - The elusive structural role of ubiquitinated histonesSusan C Moore
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
Biochem Cell Biol 80:311-9. 2002..Consequently, the search for a structural role for ubiquitinated histones continues and their effect on and importance in chromatin dynamics remains elusive... - Chromatin condensation, cysteine-rich protamine, and establishment of disulphide interprotamine bonds during spermiogenesis of Eledone cirrhosa (Cephalopoda)Pepita Gimenez-Bonafe
The Prostate Center, Vancouver General Hospital, BC, Canada
Eur J Cell Biol 81:341-9. 2002.... - Protamines, in the footsteps of linker histone evolutionJose Maria Eirin-Lopez
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
J Biol Chem 281:1-4. 2006 - Characterization of the PL-I-related SP2 protein from XenopusLindsay J Frehlick
Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 258, Victoria, B C, Canada
Biochemistry 46:12700-8. 2007..However, its interaction with circular DNA does not exhibit an enhanced preference for the supercoiled conformation, and it appears to be mainly driven by ionic interactions... - New developments in post-translational modifications and functions of histone H2A variantsAnita A Thambirajah
Department of Biochemistry and Microbiology and The Center for Biomedical Research, University of Victoria, Victoria, BCV8W3P6, Canada
Biochem Cell Biol 87:7-17. 2009..It is of additional interest that analogous sites within these different histone variants can be similarly modified. Whether this is a redundant function or a finely tuned one, designed to meet specific needs, remains to be elucidated... - sNASP, a histone H1-specific eukaryotic chaperone dimer that facilitates chromatin assemblyRon M Finn
Department of Biochemistry and Microbiology, University of Victoria, Petch Building, Victoria, BC V8W 3P6, Canada
Biophys J 95:1314-25. 2008..In vitro chromatin reconstitution experiments show that this protein facilitates the incorporation of linker histones onto nucleosome arrays and hence is a bona fide linker histone chaperone... - PL-I of Spisula solidissima, a highly elongated sperm-specific histone H1John D Lewis
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada, V8W 3P6
Biochemistry 43:7766-75. 2004..This information provides additional molecular support for the classification and underlying evolution of sperm nuclear basic proteins in bivalve molluscs... - New twists on H2A.Z: a histone variant with a controversial structural and functional pastDeanna Dryhurst
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
Biochem Cell Biol 82:490-7. 2004..The nature of these structural changes has remained somewhat controversial but nevertheless exemplifies the seemingly multifaceted nature of H2A.Z... - H2AX: tailoring histone H2A for chromatin-dependent genomic integrityAndra Li
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
Biochem Cell Biol 83:505-15. 2005.... - Physical and chemical characterization of the oligomerization state of the Aeromonas hydrophila lipase/acyltransferaseJ Ausio
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada
FEBS Lett 333:296-300. 1993..An important change occurs in the apparent partial specific volume of the molecule upon activation... - MeCP2 post-translational regulation through PEST domains: two novel hypotheses: potential relevance and implications for Rett syndromeAnita A Thambirajah
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Bioessays 31:561-9. 2009..The second hypothesis explores the use of histone deacetylase inhibitors to modulate the amounts of MeCP2 expressed in conjunction with the aforementioned therapeutic approaches... - A moment's pause: putative nucleosome-based influences on MeCP2 regulationAnita A Thambirajah
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W3P6, Canada
Biochem Cell Biol 87:791-8. 2009..By understanding the chromatin and (or) tissue context in which MeCP2 interacts, it may be possible to discern the specific etiology of diseases linked to MeCP2 dysfunction... - Protamines: structural complexity, evolution and chromatin patterningHarold E Kasinsky
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
Protein Pept Lett 18:755-71. 2011.... - Alpha-glucan recognition by a new family of carbohydrate-binding modules found primarily in bacterial pathogensAlicia Lammerts van Bueren
Department of Biochemistry and Microbiology, University of Victoria, P O Box 3055 STN CSC, Victoria, British Columbia V8W 3P6, Canada
Biochemistry 43:15633-42. 2004..e., a CBM dimer ligated by an oligosaccharide). The presence of an alpha-(1-6) linkage in the oligosaccharide appears to prevent this phenomenon...