Research Topics
| J T TrevorsSummaryCountry: Canada Publications
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Publications
Bacterial evolution and siliconJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 71:271-6. 1997..It is not unreasonable to examine the role of silicon in early molecular evolution as it may have been part of a micro-environment in which assembly of genetic information occurred...- Bacterial evolution and metabolismJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 71:257-63. 1997..This article examines the relationship between (or dependence of) bacterial evolution in prokaryotes and metabolism, and the changing physical-chemical conditions present during early evolution... - Evolution of bacterial genomesJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 71:265-70. 1997..This review examines evolution of bacterial genomes with an emphasis on RNA based life, the transition to functional DNA and small evolving genomes (possible plasmids) that led to larger, functional bacterial genomes... - Molecular evolution and optimizationJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 72:251-9. 1997..In this review, molecular evolution and molecular optimization are examined with an emphasis on the order in which evolutionary events occurred... 
Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samplesR A Carreno
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Appl Environ Microbiol 67:3309-13. 2001..Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice...- Extraction and detection of baculoviral DNA from lake water, detritus and forest litterL S England
Department of Natural Resources, Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada
J Appl Microbiol 90:630-6. 2001..SIGNIFICANCE AND IMPACT OF THE STUDY: The DNA extraction and purification protocol described in this paper will facilitate risk assessment and ecological studies of both wild-type and genetically-modified baculoviruses... - Nucleic acids in the environmentJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Curr Opin Biotechnol 7:331-6. 1996..These advances will have a significant impact on both future research and the way in which we view nucleic acids in the environment... 
One gram of soil: a microbial biochemical gene libraryJ T Trevors
School of Environmental Sciences, University of Guelph, Guelph, Ontario, NIG 2W1, Canada
Antonie Van Leeuwenhoek 97:99-106. 2010..In this article, soil as a biochemical gene library, gene expression, and the minimal number of genes for the first bacteria in the environment will be examined...- DNA in soil: adsorption, genetic transformation, molecular evolution and genetic microchipJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 70:1-10. 1996..In addition, a postulated mechanism for stabilization and elongation/assembly of primitive genetic material and the role of soil particles, salt concentrations, temperature cycling and crystal formation is examined... - Effects of 2,2',5,5'-tetrachlorobiphenyl and biphenyl on cell membranes of Ralstonia eutropha H850I S Kim
Department of Environmental Biology, University of Guelph, Guelph, ON, Canada N1G 2W1
FEMS Microbiol Lett 200:17-24. 2001..The re-adaptation to a change in temperature required only five generations to return to normal. These results show that biphenyl affects cells in more ways than simply fluidizing the cytoplasmic membrane... - Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatmentK E Shannon
Department of Environmental Biology, University of Guelph, Guelph, ON, Canada N1G 2W1
Sci Total Environ 382:121-9. 2007..This qPCR method was effective for the detection of pathogens in wastewater and confirmed that the risk of exposure to pathogens in the wastewater discharge was well within the Environment Canada guidelines... - Persistence of extracellular baculoviral DNA in aquatic microcosms: extraction, purification, and amplification by the polymerase chain reaction (PCR)L S England
Laboratory of Microbial Technology, Department of Environmental Biology, Ontario Agricultural College, University of Guelph, Guelph, Ont, Canada N1G 2W1
Mol Cell Probes 19:75-80. 2005..This study provides some of the first information on the persistence and detection limits of this viral DNA under aquatic ecological conditions, and the methods that can be used to conduct such a molecular-based field study... - Origin of microbial life hypothesis: a gel cytoplasm lacking a bilayer membrane, with infrared radiation producing exclusion zone (EZ) water, hydrogen as an energy source and thermosynthesis for bioenergeticsJ T Trevors
Laboratory of Microbiology, School of Environmental Sciences, University of Guelph, 50 Stone Rd, East, Guelph, Ontario, Canada N1G 2W1
Biochimie 94:258-62. 2012..These components will be discussed as to how they are interconnected, and their hypothesized roles in the origin of life... - Genome size in bacteriaJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 69:293-303. 1996..These aspects and selected information on physically mapping bacterial genomes will be discussed... - Phenotypic and genotypic characterization of Cryptosporidium species and isolatesR A Carreno
Department of Environmental Biology, University of Guelph, Ontario, Canada
J Ind Microbiol Biotechnol 26:95-106. 2001..However, current knowledge of Cryptosporidium genetic diversity is far from complete, and the large amount of both phenotypic and genotypic data has led to problems in our understanding of the systematics of this genus... - Bacterial gene expression at low temperaturesJ T Trevors
Laboratory of Microbiology, School of Environmental Sciences, Rm 3220 Bovey Bldg, University of Guelph, Guelph, ON, N1G 2W1, Canada
Extremophiles 16:167-76. 2012..The pros and cons of some of the molecular methodologies for gene expression analysis are also discussed... - Physical and biochemical nature of the bacterial cytoplasm: movement and localization of mRNA and the 30S subunits of ribosomesJ T Trevors
School of Environmental Sciences, Laboratory of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Prog Biophys Mol Biol 109:1-5. 2012.... - Perspective: time scales in scientific research with an emphasis on microbial cellular and molecular researchJ T Trevors
School of Environmental Sciences, Edmund Bovey Building, University of Guelph, 50 Stone Road, E Guelph, Ontario, Canada N1G 2W1
J Microbiol Methods 82:102-7. 2010.... - Suitable microscopic entropy for the origin of microbial life: microbiological methods are challengesJ T Trevors
School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Microbiol Methods 83:341-4. 2010..Methodology limitations to study postulated past origin of microbial life events and to mimic these events in the laboratory, are still obstacles to understanding the origin of life... - Microarray analysis of Escherichia coli strains from interstitial beach waters of Lake Huron (Canada)T Kon
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Appl Environ Microbiol 73:7757-8. 2007..This suggested that adaptation and growth occurred within the interstitial water sites tested. All isolates were nonpathogenic, and three lake isolates possessed tetracycline resistance genes... - Real-time polymerase chain reaction quantification of the transgenes for roundup ready corn and roundup ready soybean in soil samplesS Lerat
Department of Environmental Biology, of Plant Agriculture, and of Botany, University of Guelph, Guelph, Ontario, Canada
J Agric Food Chem 53:1337-42. 2005..As few as one copy of RR corn genome or one copy of RR soybean genome was detected in the soil DNA extract... - Origin of microbial life: Nano- and molecular events, thermodynamics/entropy, quantum mechanisms and genetic instructionsJ T Trevors
School of Environmental Sciences, University of Guelph, 50 Stone Rd, East, Guelph, Ontario, N1G 2W1, Canada
J Microbiol Methods 84:492-5. 2011.... - Chance and necessity do not explain the origin of lifeJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, Room 3220, Bovey Building, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
Cell Biol Int 28:729-39. 2004..Minimal metabolism would be needed for cells to be capable of growth and division. All known metabolism is cybernetic--that is, it is programmatically and algorithmically organized and controlled... - DNA technologies: what's next applied to microbiology research?J T Trevors
School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Antonie Van Leeuwenhoek 98:249-62. 2010..What's next with DNA is not always obvious, but history shows the international microbiology research community will readily adopt it... - Cytoplasmic membrane polarization in Gram-positive and Gram-negative bacteria grown in the absence and presence of tetracyclineM Vincent
Laboratory of Microbial Technology, Rm 3215 Bovey Building, Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Biochim Biophys Acta 1672:131-4. 2004..To our knowledge, these are some of the first cytoplasmic membrane polarization values reported for these Gram-negative and Gram-positive bacteria over a broad temperature range and also for cells grown in the presence of tetracycline... - Growth and membrane polarization in Pseudomonas aeruginosa UG2 grown in randomized microgravity in a high aspect ratio vesselL S England
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, ON, Canada N1G 2W1
Biochim Biophys Acta 1624:76-80. 2003..It may be possible that RMG effects are species-dependent or bacterial cells with a small mass and volume may be near the threshold where RMG exerts a minimal effect... - Molecular evolution in bacteria: surfaces, cathodes and anodesJ T Trevors
Department of Environmental Biology, University of Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 71:363-8. 1997..These aspects will be considered from a theoretical evolutionary perspective... - Extraction, detection and persistence of extracellular DNA in forest litter microcosmsL S England
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Room 3220, Bovey Building, Guelph, Ont, Canada N1G 2W1
Mol Cell Probes 18:313-9. 2004..A 530 bp DNA fragment from the genome of the baculovirus CfMNPVegt(-)/lacZ(+) was detected in these microcosms for about 3 months. The DNA may have persisted for a longer period but was below the detection limit of the PCR analysis... - A perspective on the mobilization, localization and delivery of molecules in the crowded bacterial cytoplasmJ T Trevors
Laboratory of Microbiology, School of Environmental Sciences, University of Guelph, 50 Stone Rd, East, Guelph, Ontario, N1G 2W1, Canada
Curr Issues Mol Biol 14:39-46. 2012.... - Quantum microbiologyJ T Trevors
School of Environmental Sciences, University of Guelph, Ontario, Canada
Curr Issues Mol Biol 13:43-50. 2011.... - Can dead bacterial cells be defined and are genes expressed after cell death?J T Trevors
Laboratory of Microbiology, School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Microbiol Methods 90:25-8. 2012..The definition of living and dead has implications for possible, completely, synthetic bacterial cells that may be capable of growth and division... - Introduction of green fluorescent protein gene into phenol-degrading Alcaligenes faecalis cells and their monitoring in phenol-contaminated soilA E Bastos
Department of Environmental Biology, University of Guelph, Ontario, Canada
Appl Microbiol Biotechnol 56:255-60. 2001..Survival of gfp-marked cells in phenol-contaminated soil over 22 days was determined from plate counts using an epifluorescence microscope... - Synthesis of ligand-specific phage-display ScFv against the herbicide picloram by direct cloning from hyperimmunized mouseN L Tout
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Agric Food Chem 49:3628-37. 2001..5 Da, predominantly requires somatic hypermutations... - Molecular evolution: first enzymes, gases as substrates and genetic templatesJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2WI
Riv Biol 94:105-22. 2001.... - Improving the rate of infectivity of Cryptosporidium parvum oocysts in cell culture using centrifugationS C Weir
Department of Environmental Biology, University of Guelph, Ontario, Canada
J Parasitol 87:1502-4. 2001..Centrifugation may expedite detection during C. parvum infectivity assays. Furthermore, multiwell plate formats are more cost effective than traditional chamber slides... - Generalizations about bacteriology: thermodynamic, open systems, genetic instructions, and evolutionJ T Trevors
School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada
Antonie Van Leeuwenhoek 97:313-8. 2010..Some fundamental properties describing bacterial life are also presented... - Self-assembly of the first cells in a hydrophobic environment with hydrogen as the energy sourceJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
Riv Biol 94:535-41. 2001..The possibility is explored that life first self-assembled in a hydrophobic environment in the subsurface protected from radiation with ubiquitous hydrogen as the likely universal energy source... - Possible origin of a membrane in the subsurface of the EarthJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Room 3220 Bovey Building, ON, Guelph, Canada N1G 2W1
Cell Biol Int 27:451-7. 2003..Such an open system could also acquire novel functions, whereas a closed system would be unable to evolve... - The Big Bang, Superstring Theory and the origin of life on the EarthJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Theory Biosci 124:403-12. 2006..It was the separation of the unified force into four singular forces that allowed the origin of life... - Fluorescent methods to study DNA, RNA, proteins and cytoplasmic membrane polarization in the pentachlorophenol-mineralizing bacterium Sphingomonas sp. UG30 during nutrient starvation in waterT J Denich
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
J Fluoresc 15:143-51. 2005.... - Fluorescent probes for bacterial cytoplasmic membrane researchJ T Trevors
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
J Biochem Biophys Methods 57:87-103. 2003.... - Early assembly of cellular lifeJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, Ont, Canada N1G 2W1
Prog Biophys Mol Biol 81:201-17. 2003..This may have been widespread in the subsurface of the early Earth located at microscopic physical domains... - Microbial processes in the Athabasca Oil Sands and their potential applications in microbial enhanced oil recoveryN K Harner
Laboratory of Microbiology, School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada
J Ind Microbiol Biotechnol 38:1761-75. 2011..This article reviews the microbiology of the Athabasca Oil Sands and the mechanisms bacteria use to tolerate low water and high hydrocarbon availability in oil reservoirs and oilfields, and potential applications in MEOR... - Viable but non-culturable (VBNC) bacteria: Gene expression in planktonic and biofilm cellsJ T Trevors
Laboratory of Microbiology, School of Environmental Sciences, Rm 3320 Bovey Building, University of Guelph, 50 Stone Rd, East, Guelph, Ontario, Canada N1G 2W1
J Microbiol Methods 86:266-73. 2011.... - How much cytoplasm can a bacterial genome control?J T Trevors
School of Environmental Sciences, University of Guelph, 50 Stone Road, E, Guelph, Ontario, Canada
J Microbiol Methods 84:147-50. 2011..The optimal genome size is one that is relatively constant, but also has some plasticity for evolutionary change (via gene transfer) and mutational events, and can control a range of cytoplasm during the cell cycle... - Perspective: researching the transition from non-living to the first microorganisms: methods and experiments are major challengesJ T Trevors
School of Environmental Sciences, University of Guelph, 350 Stone Rd, East, Guelph, Ontario NIG2W1, Canada
J Microbiol Methods 81:259-63. 2010..These perspectives will be discussed in this article as well as the possibility that a pre-biotic film preceded a microbial biofilm as a possible micro-location for the origin of microbial cells capable of growth and division... - From self-assembly of life to present-day bacteria: a possible role for nanocellsJ T Trevors
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
FEMS Microbiol Rev 25:573-82. 2001..This will also be examined from an evolutionary perspective with hydrogen as the postulated universal energy source [Morita, R. (2000) Microb. Ecol. 38, 307-320]...