Janet Rossant

Summary

Affiliation: The Hospital for Sick Children
Country: Canada

Publications

  1. ncbi request reprint Stem cells and lineage development in the mammalian blastocyst
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Reprod Fertil Dev 19:111-8. 2007
  2. ncbi request reprint Stem cells: the magic brew
    Janet Rossant
    Nature 448:260-2. 2007
  3. pmc Loss of Notch signalling induced by Dll4 causes arterial calibre reduction by increasing endothelial cell response to angiogenic stimuli
    Rui Benedito
    Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinaria, Technical University of Lisbon, Lisboa, Portugal
    BMC Dev Biol 8:117. 2008
  4. ncbi request reprint Retrospective. Anne McLaren (1927-2007)
    Janet Rossant
    Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 1X8
    Science 317:609. 2007
  5. doi request reprint The impact of developmental biology on pluripotent stem cell research: successes and challenges
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
    Dev Cell 21:20-3. 2011
  6. doi request reprint Stem cells and early lineage development
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Department of Molecular Genetics, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
    Cell 132:527-31. 2008
  7. doi request reprint Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
    Janet Rossant
    Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
    Development 136:701-13. 2009
  8. pmc A developmental journey and lessons learned along the way
    Janet Rossant
    The Hospital for Sick Children, Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada
    Mol Biol Cell 21:9-10. 2010
  9. doi request reprint Reprogramming to pluripotency: from frogs to stem cells
    Janet Rossant
    Hospital for Sick Children Research Institute, University of Toronto, Ontario, Canada
    Cell 138:1047-50. 2009
  10. pmc Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Dev Cell 22:887-901. 2012

Detail Information

Publications103 found, 100 shown here

  1. ncbi request reprint Stem cells and lineage development in the mammalian blastocyst
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Reprod Fertil Dev 19:111-8. 2007
    ....
  2. ncbi request reprint Stem cells: the magic brew
    Janet Rossant
    Nature 448:260-2. 2007
  3. pmc Loss of Notch signalling induced by Dll4 causes arterial calibre reduction by increasing endothelial cell response to angiogenic stimuli
    Rui Benedito
    Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinaria, Technical University of Lisbon, Lisboa, Portugal
    BMC Dev Biol 8:117. 2008
    ..Dll4 null embryos die very early in development with severely reduced arterial calibre and lumen and loss of arterial cell identity...
  4. ncbi request reprint Retrospective. Anne McLaren (1927-2007)
    Janet Rossant
    Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 1X8
    Science 317:609. 2007
  5. doi request reprint The impact of developmental biology on pluripotent stem cell research: successes and challenges
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
    Dev Cell 21:20-3. 2011
    ..However, details of developmental pathways differ even across mammalian species. Full realization of the potential of stem cells will require more direct studies of human or primate developmental biology...
  6. doi request reprint Stem cells and early lineage development
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Department of Molecular Genetics, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
    Cell 132:527-31. 2008
    ..Understanding the initial developmental status of the different pluripotent lines is critical for defining starting conditions for differentiation toward therapeutically relevant cell types...
  7. doi request reprint Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
    Janet Rossant
    Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
    Development 136:701-13. 2009
    ..We also discuss the continuing debate that surrounds the relative impacts of early lineage bias versus the stochastic allocation of cells with respect to the events that pattern the blastocyst and initiate its later asymmetries...
  8. pmc A developmental journey and lessons learned along the way
    Janet Rossant
    The Hospital for Sick Children, Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada
    Mol Biol Cell 21:9-10. 2010
    ..We also need to use our science skills to contribute to public debate on complex issues of the day...
  9. doi request reprint Reprogramming to pluripotency: from frogs to stem cells
    Janet Rossant
    Hospital for Sick Children Research Institute, University of Toronto, Ontario, Canada
    Cell 138:1047-50. 2009
    ..This year's Albert Lasker Basic Medical Research Award goes to John Gurdon and Shinya Yamanaka for their contributions to our understanding of how to reprogram adult cells back to early embryonic states...
  10. pmc Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Dev Cell 22:887-901. 2012
    ..These results provide a comprehensive stem cell proteomic resource and enable new approaches to interrogate the mechanisms that regulate cell fate specification...
  11. doi request reprint Porcn-dependent Wnt signaling is not required prior to mouse gastrulation
    Steffen Biechele
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, ON M5G 1X8, Canada
    Development 140:2961-71. 2013
    ....
  12. ncbi request reprint Disruption of early proximodistal patterning and AVE formation in Apc mutants
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Development 133:3379-87. 2006
    ..In addition, we found that nuclear beta-catenin and other molecular markers are asymmetrically expressed by 4.5 days...
  13. ncbi request reprint Live imaging and genetic analysis of mouse notochord formation reveals regional morphogenetic mechanisms
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Dev Cell 13:884-96. 2007
    ..Therefore, we propose three distinct regions within the mouse notochord, each with unique morphogenetic origins...
  14. doi request reprint Hedgehog regulates distinct vascular patterning events through VEGF-dependent and -independent mechanisms
    Leigh Coultas
    Developmental and Stem Cell Biology Program, Hospital for Sick Children Research Institute, 555 University Ave, Toronto, Ontario, Canada
    Blood 116:653-60. 2010
    ..These findings establish that Hh uses both VEGF- and Notch-dependent and -independent mechanisms to pattern specific events in early blood vascular development...
  15. ncbi request reprint Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst
    Dan Strumpf
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Ontario, Canada
    Development 132:2093-102. 2005
    ..Thus, Cdx2 is essential for segregation of the ICM and TE lineages at the blastocyst stage by ensuring repression of Oct4 and Nanog in the TE...
  16. pmc Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry
    Jun K Takeuchi
    Department of Cardiovascular Research, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    Proc Natl Acad Sci U S A 104:846-51. 2007
    ..Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry...
  17. doi request reprint Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, Toronto, ON M5G 1L7, Canada
    Development 137:395-403. 2010
    ....
  18. ncbi request reprint Vascular endothelial growth factor directly inhibits primitive neural stem cell survival but promotes definitive neural stem cell survival
    Tamaki Wada
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 3E1
    J Neurosci 26:6803-12. 2006
    ..In conclusion, VEGF-A function through Flk1 mediates survival (and not proliferative or fate change) effects on NSCs, specifically...
  19. ncbi request reprint Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, 101 College St, Toronto, ON, Canada
    Dev Biol 313:614-29. 2008
    ..Cdx2 therefore appears to act downstream of the first lineage decision, suggesting that processes influencing lineage allocation or morphogenesis may regulate Cdx2 expression along the inside/outside axis of the embryo...
  20. pmc Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes
    Brian Cox
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada
    Mol Syst Biol 3:109. 2007
    ..These targets are evidence that Nmyc is a regulator of genes involved in mRNA processing and a repressor of the imprinted gene Igf2r in the developing lung...
  21. ncbi request reprint Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers...
  22. doi request reprint Porcupine homolog is required for canonical Wnt signaling and gastrulation in mouse embryos
    Steffen Biechele
    Department of Molecular Genetics, University of Toronto, ON, Canada
    Dev Biol 355:275-85. 2011
    ..Taken together, these data confirm the importance of Porcn for Wnt secretion and gastrulation and suggest that disruption of early development underlies the male lethality of human PORCN mutants...
  23. doi request reprint Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin
    Robert Odell Stephenson
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Development 137:3383-91. 2010
    ..They also show that Cdx2 expression is strongly linked to apical membrane polarization...
  24. ncbi request reprint Baf60c is essential for function of BAF chromatin remodelling complexes in heart development
    Heiko Lickert
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nature 432:107-12. 2004
    ....
  25. doi request reprint The Hippo pathway member Nf2 is required for inner cell mass specification
    Katie Cockburn
    Department of Molecular Genetics, University of Toronto, ON M5S 1A8, Canada
    Curr Biol 23:1195-201. 2013
    ..Together, these data establish a clear role for Nf2 upstream of Yap in the preimplantation embryo and demonstrate that Hippo signaling is essential to segregate the ICM from the TE. ..
  26. pmc Translational analysis of mouse and human placental protein and mRNA reveals distinct molecular pathologies in human preeclampsia
    Brian Cox
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
    Mol Cell Proteomics 10:M111.012526. 2011
    ..We conclude that clinically similar preeclampsia patients exhibit divergent placental gene expression profiles thus implicating divergent molecular mechanisms in the origins of this disease...
  27. ncbi request reprint Foxh1 is essential for development of the anterior heart field
    Ingo Von Both
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Dev Cell 7:331-45. 2004
    ..Thus, Foxh1 and Nkx2-5 functionally interact and are essential for development of the AHF and its derivatives, the RV and OFT, in response to TGFbeta-like signals...
  28. doi request reprint Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
    Nat Methods 6:370-6. 2009
    ..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2...
  29. pmc Comparative systems biology of human and mouse as a tool to guide the modeling of human placental pathology
    Brian Cox
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
    Mol Syst Biol 5:279. 2009
    ..The entire data are available as a web-accessible database to guide the informed development of mouse models to study human disease...
  30. ncbi request reprint Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis development
    Pantelis Georgiades
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Development 133:1059-68. 2006
    ..This study provides new insights about the genetic and cellular basis of the patterning role and development of the early trophoblast...
  31. pmc Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis
    Keli Xu
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
    Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation...
  32. ncbi request reprint Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling
    Thomas Kislinger
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada
    Cell 125:173-86. 2006
    ..This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal...
  33. pmc Zfp206 regulates ES cell gene expression and differentiation
    Wen Zhang
    Department of Molecular and Medical Genetics, University of Toronto, 4388 Medical Sciences Building, 1 King s College Circle, Toronto, ON M5S 1A8, Canada
    Nucleic Acids Res 34:4780-90. 2006
    ..Together, these data indicate that Zfp206 is a regulator of ES cell differentiation that controls a set of genes expressed very early in development, most of which themselves appear to be regulators...
  34. doi request reprint A novel Phex mutation in a new mouse model of hypophosphatemic rickets
    Celeste Owen
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Cell Biochem 113:2432-41. 2012
    ....
  35. pmc Making the blastocyst: lessons from the mouse
    Katie Cockburn
    Department of Molecular Genetics, University of Toronto, Canada
    J Clin Invest 120:995-1003. 2010
    ..A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells...
  36. ncbi request reprint Genome-wide analysis of mouse transcripts using exon microarrays and factor graphs
    Brendan J Frey
    Electrical and Computer Engineering, University of Toronto, 10 King s College Rd, Toronto, Ontario M5S 3G4, Canada
    Nat Genet 37:991-6. 2005
    ..GenRate also detected tens of thousands of potential new exons and reconciled discrepancies in current cDNA databases by 'stitching' new transcribed regions into previously annotated genes...
  37. pmc Cdx2 is essential for axial elongation in mouse development
    Kallayanee Chawengsaksophak
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Proc Natl Acad Sci U S A 101:7641-5. 2004
    ..The gene appears to be important in the integration of the pathways controlling embryonic axial elongation, and anterior-posterior patterning...
  38. ncbi request reprint Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors
    Masatsugu Ema
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Blood 107:111-7. 2006
    ..Thus, Flk1 expression marks progenitors with broad mesodermal potential but is not absolutely required for the development of all mesodermal lineages in which it is expressed...
  39. pmc Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryo
    Kashif Ahmed
    Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada
    PLoS ONE 5:e10531. 2010
    ..Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells...
  40. ncbi request reprint Activated Fps/Fes partially rescues the in vivo developmental potential of Flk1-deficient vascular progenitor cells
    Jody J Haigh
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Ave, Toronto, Ontario, Canada M5G 1X5
    Blood 103:912-20. 2004
    ..We have also demonstrated that activated Fps/Fes causes hemangioma formation in vivo, independently of Flk1, as a result of increasing vascular progenitor density...
  41. doi request reprint FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Development 137:715-24. 2010
    ..In conclusion, we propose a model in which stochastic and progressive specification of EPI and PE lineages occurs during maturation of the blastocyst in an FGF/MAP kinase signal-dependent manner...
  42. doi request reprint Location of transient ectodermal progenitor potential in mouse development
    Lingyu Li
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Development 140:4533-43. 2013
    ..Our work not only improves our understanding of ectodermal layer development in early embryos, but also provides a framework for regenerative differentiation towards ectodermal tissues. ..
  43. doi request reprint The role of FGF/Erk signaling in pluripotent cells
    Fredrik Lanner
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Development 137:3351-60. 2010
    ..Understanding how extrinsic signals such as FGF regulate different stem cell states will be crucial to harvest the clinical promise of induced pluripotent and embryo-derived stem cells...
  44. pmc Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, ON, Canada
    Proc Natl Acad Sci U S A 107:10783-90. 2010
    ..These studies indicate that histone modification mechanisms may differ between early embryo lineages and emphasize the importance of examining in vivo and in vitro progenitor cells...
  45. pmc Lineage allocation and asymmetries in the early mouse embryo
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Philos Trans R Soc Lond B Biol Sci 358:1341-8; discussion 1349. 2003
    ....
  46. pmc Enu mutagenesis identifies a novel platelet phenotype in a loss-of-function Jak2 allele
    Nicole M Anderson
    Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 8:e75472. 2013
    ..Our data suggest that perturbing JAK2 activation may have unexpected consequences in elevation of platelet number and correspondingly, important implications for treatment of hematological disorders with constitutive Jak2 activity...
  47. doi request reprint Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord
    Owen J Tamplin
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    Dev Biol 360:415-25. 2011
    ..The combination of highly specific gene expression profiling and genome-wide ChIP analysis is a powerful means of understanding developmental pathways, even for small cell populations such as the notochord...
  48. pmc Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives
    Owen J Tamplin
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    BMC Genomics 9:511. 2008
    ..However, the targets of Foxa2 during embryogenesis, and the molecular impact of organizer loss on the gastrula embryo, have not been well defined...
  49. doi request reprint Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells
    Cheryle A Séguin
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1L7, Canada
    Cell Stem Cell 3:182-95. 2008
    ..These endoderm progenitor cells maintain a stable phenotype through many passages in culture, thereby providing new tools to explore the pathways of endoderm differentiation...
  50. ncbi request reprint Lineage development and polar asymmetries in the peri-implantation mouse blastocyst
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinal Hospital, Toronto, Ont, Canada
    Semin Cell Dev Biol 15:573-81. 2004
    ..The exact relationship between lineage segregation, preimplantation polarities and the postimplantation axes remain to be determined but are now open to molecular and cellular investigation...
  51. ncbi request reprint Adhesion is prerequisite, but alone insufficient, to elicit stem cell pluripotency
    Phillip Karpowicz
    Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Neurosci 27:5437-47. 2007
    ..These adhesive differences suggest an evolving compartmentalization in multipotent NSCs during development and serve to illustrate the importance of cell-cell association for revealing cellular contribution...
  52. pmc High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivo in mice
    John C Slevin
    Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Canada
    BMC Dev Biol 6:10. 2006
    ..This method may be useful for determining the developmental effects of targeted genetic or cellular interventions at critical stages of placentation, gastrulation, axis formation, and neural tube closure...
  53. pmc Three-dimensional analysis of vascular development in the mouse embryo
    Johnathon R Walls
    Mouse Imaging Centre, Hospital for Sick Children, Toronto Centre for Phenogenomics, Toronto, Ontario, Canada
    PLoS ONE 3:e2853. 2008
    ..0 and E10.0. This atlas is freely available at http://www.mouseimaging.ca/research/mouse_atlas.html and will serve as a platform to provide insight into normal and abnormal vascular development...
  54. pmc Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos
    Marina Gertsenstein
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 5:e11260. 2010
    ..Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources...
  55. doi request reprint The V-ATPase a3 subunit mutation R740S is dominant negative and results in osteopetrosis in mice
    Noelle Ochotny
    Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
    J Bone Miner Res 26:1484-93. 2011
    ..These results suggest that the V(0) complex has proton-pumping-independent functions in mammalian cells...
  56. ncbi request reprint Mouse trophoblast stem cells
    Jennifer Quinn
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Ontario, Canada
    Methods Mol Med 121:125-48. 2006
    ..This chapter will discuss the initial derivation of trophoblast stem cells from the blastocyst stage, maintenance, differentiation, flow cytometry and transfection techniques that can be used with these cells...
  57. pmc Trophoblast expression of fms-like tyrosine kinase 1 is not required for the establishment of the maternal-fetal interface in the mouse placenta
    Masanori Hirashima
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, ON, Canada M5G 1X5
    Proc Natl Acad Sci U S A 100:15637-42. 2003
    ..Targeting maternal sFlt1 levels for treatment of preeclampsia may thus be possible without affecting the proper formation of the placenta...
  58. ncbi request reprint Endothelial cells and VEGF in vascular development
    Leigh Coultas
    The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8 Canada
    Nature 438:937-45. 2005
    ....
  59. ncbi request reprint Cell and molecular regulation of the mouse blastocyst
    Yojiro Yamanaka
    Program of Developmental Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Dev Dyn 235:2301-14. 2006
    ..Together, these mechanisms guide the differentiation of the first lineages of the mouse and thereby set up tissues that will be important for the first steps of embryonic body patterning...
  60. ncbi request reprint Gene trapping in embryonic stem cells
    William L Stanford
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Methods Enzymol 420:136-62. 2006
    ....
  61. ncbi request reprint Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein
    Amy P Wong
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Nat Biotechnol 30:876-82. 2012
    ..Our study provides a method for generating patient-specific airway epithelial cells for disease modeling and in vitro drug testing...
  62. pmc Cytopenia induction by 5-fluorouracil identifies thrombopoietic mutants in sensitized ENU mutagenesis screens
    Nicole M Anderson
    Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ont, Canada
    Exp Hematol 40:48-60. 2012
    ....
  63. doi request reprint The genetics of induced pluripotency
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Reproduction 139:35-44. 2010
    ..Knowledge of the requirement for these genes in the embryo and in embryonic stem cells will advance our understanding of how to reverse the developmental clock for therapeutic benefit...
  64. ncbi request reprint Two mouse mutations mapped to chromosome 11 with differing morphologies but similar progressive inflammatory alopecia
    Geoffrey A Wood
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Exp Dermatol 14:373-9. 2005
    ..The early onset of the disease, predictable progression, and differences in inflammatory cell localization between the two mutants make these mice particularly useful models for inflammatory hair loss and autoimmune diseases in general...
  65. ncbi request reprint Emerging asymmetry and embryonic patterning in early mouse development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Ontario M5G 1X5, Canada
    Dev Cell 7:155-64. 2004
    ..Whether these asymmetries are causally related to embryonic patterning or are coincidental outcomes of the topology of normal development remains uncertain...
  66. ncbi request reprint Gene-trap expression screening to identify endothelial-specific genes
    Masanori Hirashima
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Blood 104:711-8. 2004
    ..Gene-trap expression screening can thus be used to identify early endothelial-specific genes and analyze their function in mice...
  67. ncbi request reprint How signaling promotes stem cell survival: trophoblast stem cells and Shp2
    Amy Ralston
    The Hospital for Sick Children, and Department of Medical Genetics and Microbiology, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    Dev Cell 10:275-6. 2006
    ..These studies provide a novel link between FGF/Erk signaling and cell survival that may be relevant to other stem and progenitor cell niches...
  68. doi request reprint The Crumbs complex couples cell density sensing to Hippo-dependent control of the TGF-β-SMAD pathway
    Xaralabos Varelas
    Center for Systems Biology, Mount Sinai Hospital, Toronto, ON, Canada
    Dev Cell 19:831-44. 2010
    ..Accordingly, disruption of the Crumbs complex enhances TGF-β signaling and predisposes cells to TGF-β-mediated epithelial-to-mesenchymal transitions...
  69. pmc Phenotypic annotation of the mouse X chromosome
    Brian J Cox
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1L7, Canada
    Genome Res 20:1154-64. 2010
    ..The entire data set is available via the publicly accessible website (http://xlinkedgenes.ibme.utoronto.ca/)...
  70. pmc The functional landscape of mouse gene expression
    Wen Zhang
    Banting and Best Department of Medical Research, University of Toronto, 1 King s College Circle, Toronto, ON M5S 1A8, Canada
    J Biol 3:21. 2004
    ....
  71. doi request reprint Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state
    Fredrik Lanner
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    Stem Cells 28:191-200. 2010
    ..We conclude that N-sulfated HS is required for FGF4 signaling to maintain ES cells primed for differentiation in a heterogeneous state. Inhibiting this pathway facilitates a more naïve ground state...
  72. doi request reprint The challenge of regulating rapidly changing science: stem cell legislation in Canada
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Cell Stem Cell 4:285-8. 2009
    ..We encourage scientists and policymakers to collaborate to ensure a clear regulatory framework that accommodates future advances...
  73. ncbi request reprint Vascular development and patterning: making the right choices
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Curr Opin Genet Dev 13:408-12. 2003
    ....
  74. ncbi request reprint Cell fate decisions in early blood vessel formation
    Masatsugu Ema
    Samuel Lunenfeld Research Institute and the Department of Molecular and Medical Genetics, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Trends Cardiovasc Med 13:254-9. 2003
    ....
  75. ncbi request reprint Magnetic resonance imaging for detection and analysis of mouse phenotypes
    Brian J Nieman
    Mouse Imaging Centre, Hospital for Sick Children, Toronto, Canada
    NMR Biomed 18:447-68. 2005
    ..In particular, the needs for high-throughput imaging and comparative anatomical analyses in large biological studies are described and current efforts at handling these issues are presented...
  76. ncbi request reprint Spatial and temporal patterns of ERK signaling during mouse embryogenesis
    Laura Beth Corson
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Development 130:4527-37. 2003
    ..These studies identify key domains of sustained ERK signaling in the intact mouse embryo, give significant insight into the regulation of this signaling in vivo and pinpoint regions where downstream target genes can be sought...
  77. ncbi request reprint Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype
    Tilo Kunath
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5
    Nat Biotechnol 21:559-61. 2003
    ..Such embryos, in which endogenous p120-Ras GTPase-activating protein (RasGAP), encoded by Rasa1 (also known as RasGAP), was silenced, had the same phenotype as did the previously reported Rasa1 null mutation...
  78. pmc The Centre for Modeling Human Disease Gene Trap resource
    Christine To
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 32:D557-9. 2004
    ..asp). The clones are available as a resource to researchers worldwide to help to functionally annotate the mammalian genome and will serve as a source to test candidate loci identified by phenotype-driven mutagenesis screens...
  79. ncbi request reprint A novel in vitro model of trophoblast-mediated decidual blood vessel remodeling
    Caroline Dunk
    Departments of Physiology and Fetal and Maternal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Lab Invest 83:1821-8. 2003
    ....
  80. ncbi request reprint Molecular profiling of non-small cell lung cancer and correlation with disease-free survival
    Dennis A Wigle
    Thoracic Oncology Site Group, Princess Margaret Hospital, Samuel Lunenfeld Research Institute of Mount Sinai Hospital, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Cancer Res 62:3005-8. 2002
    ..The results provide evidence that molecular subtyping of NSCLC can identify distinct profiles of gene expression correlating with disease-free survival...
  81. ncbi request reprint Signaling pathways in vascular development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Molecular and Medical Genetics, University of Toronto, 600 University Avenue, Ontario, M5G 1X5, Canada
    Annu Rev Cell Dev Biol 18:541-73. 2002
    ..Further understanding of the complexities of the pathways should help refine such strategies...
  82. pmc The mouse genome sequence--the end of the tail, or just the beginning?
    Janet Rossant
    Samuel Lunenfeld Research Institute, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Genome Biol 4:109. 2003
    ..Each illustrates how the availability of the genome sequence will change the way mouse biologists do business in future...
  83. ncbi request reprint A Gja1 missense mutation in a mouse model of oculodentodigital dysplasia
    Ann M Flenniken
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Development 132:4375-86. 2005
    ..Thus, these mice represent an experimental model with which to explore the clinical manifestations of ODDD and to evaluate potential intervention strategies...
  84. ncbi request reprint PRISM, a generic large scale proteomic investigation strategy for mammals
    Thomas Kislinger
    Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada
    Mol Cell Proteomics 2:96-106. 2003
    ..PRISM therefore offers an effective platform for in-depth investigation of complex mammalian proteomes...
  85. pmc Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity
    Tetsuya S Tanaka
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, 21224 6820, USA
    Genome Res 12:1921-8. 2002
    ..We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage...
  86. ncbi request reprint Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
    ..This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development...
  87. ncbi request reprint Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst
    François Gerbe
    INSERM, UMR384, Faculte de Medecine, 28 place H Dunant BP38, Clermont Ferrand 63001, Universite d Auvergne, Clermont Fd, France
    Dev Biol 313:594-602. 2008
    ..Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps...
  88. ncbi request reprint Remembering Rosa Beddington--a tribute from her friends and colleagues
    Paul F Goetinck
    Dev Dyn 223:3-11. 2002
  89. pmc Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse
    Masatsugu Ema
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada M5G 1X5
    Genes Dev 17:380-93. 2003
    ..We propose a model in which the combinatorial effects of Flk1 and Tal1 act to regulate cell fate choice in early development into hematopoietic, endothelial, and smooth muscle lineages...
  90. ncbi request reprint Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH gene
    J Henri Bayle
    Howard Hughes Medical Institute and the Department Pathology, Beckman Center for Molecular and Genetic Medicine, Stanford University, Stanford, California 94305, USA
    J Biol Chem 277:28884-91. 2002
    ..DCoH function as it pertains to HNF1 activity appears to be partially complemented by a newly identified homolog, DCoH2...
  91. ncbi request reprint A monoclonal mouse?
    Janet Rossant
    Nature 415:967-9. 2002
  92. ncbi request reprint Stem cells. Setting standards for human embryonic stem cells
    Ali H Brivanlou
    Rockefeller University, New York, NY 10021, USA
    Science 300:913-6. 2003
  93. ncbi request reprint Control of effector CD8+ T cell function by the transcription factor Eomesodermin
    Erika L Pearce
    Abramson Family Cancer Research Institute, and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 302:1041-3. 2003
    ..We suggest that Eomesodermin is likely to complement the actions of T-bet and act as a key regulatory gene in the development of cell-mediated immunity...
  94. ncbi request reprint Trophoblast-specific gene manipulation using lentivirus-based vectors
    Pantelis Georgiades
    Developmental Genetics and Embryology Research Unit, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus
    Biotechniques 42:317-8, 320, 322-5. 2007
    ..This lentivirus lineage-specific infection system thus facilitates both gain and loss of function studies during placental development in the mouse and potentially other mammalian species...
  95. ncbi request reprint Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
    Oluseun Adewumi
    UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    Nat Biotechnol 25:803-16. 2007
    ..Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected...
  96. ncbi request reprint The mouse embryo autonomously acquires anterior-posterior polarity at implantation
    Katsuyoshi Takaoka
    Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Dev Cell 10:451-9. 2006
    ..This asymmetry in molecular patterning of the mouse embryo pushes back the origin of the A-P body axis to the peri-implantation stage...
  97. ncbi request reprint Ethics. The ISSCR guidelines for human embryonic stem cell research
    George Q Daley
    Children s Hospital, Boston, Massachusetts, USA
    Science 315:603-4. 2007
  98. ncbi request reprint The International Stem Cell Initiative: toward benchmarks for human embryonic stem cell research
    Peter W Andrews
    Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
    Nat Biotechnol 23:795-7. 2005
  99. ncbi request reprint Connexin31-deficient trophoblast stem cells: a model to analyze the role of gap junction communication in mouse placental development
    Mark Kibschull
    Institute of Anatomy, University Hospital Duisburg Essen, 45122, Germany
    Dev Biol 273:63-75. 2004
    ..We conclude that during trophoblast cell lineage differentiation, the Cx31 gap junction channel is involved in maintaining the proliferative diploid trophoblast cell population...
  100. ncbi request reprint Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts
    Tilo Kunath
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada
    Development 132:1649-61. 2005
    ..Based on gene expression analysis, chimera studies and imprinted X-inactivation, XEN cell lines are representative of extra-embryonic endoderm and provide a new cell culture model of an early mammalian lineage...
  101. pmc Transcriptome analysis of mouse stem cells and early embryos
    Alexei A Sharov
    National Institute on Aging, Baltimore, Maryland, USA
    PLoS Biol 1:E74. 2003
    ..The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine...