Research Topics
Genomes and Genes
| Janet RossantSummaryAffiliation: The Hospital for Sick Children Country: Canada Publications
| Collaborators
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Detail Information
Publications
Stem cells and lineage development in the mammalian blastocystJanet Rossant
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
Reprod Fertil Dev 19:111-8. 2007....- Stem cells: the magic brewJanet Rossant
Nature 448:260-2. 2007 
Loss of Notch signalling induced by Dll4 causes arterial calibre reduction by increasing endothelial cell response to angiogenic stimuliRui Benedito
Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinaria, Technical University of Lisbon, Lisboa, Portugal
BMC Dev Biol 8:117. 2008..Dll4 null embryos die very early in development with severely reduced arterial calibre and lumen and loss of arterial cell identity...- A developmental journey and lessons learned along the wayJanet Rossant
The Hospital for Sick Children, Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada
Mol Biol Cell 21:9-10. 2010..We also need to use our science skills to contribute to public debate on complex issues of the day... 
Reprogramming to pluripotency: from frogs to stem cellsJanet Rossant
Hospital for Sick Children Research Institute, University of Toronto, Ontario, Canada
Cell 138:1047-50. 2009..This year's Albert Lasker Basic Medical Research Award goes to John Gurdon and Shinya Yamanaka for their contributions to our understanding of how to reprogram adult cells back to early embryonic states...- Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouseJanet Rossant
Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
Development 136:701-13. 2009..We also discuss the continuing debate that surrounds the relative impacts of early lineage bias versus the stochastic allocation of cells with respect to the events that pattern the blastocyst and initiate its later asymmetries... - Retrospective. Anne McLaren (1927-2007)Janet Rossant
Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada. M5G 1X8
Science 317:609. 2007 - Stem cells and early lineage developmentJanet Rossant
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Department of Molecular Genetics, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
Cell 132:527-31. 2008..Understanding the initial developmental status of the different pluripotent lines is critical for defining starting conditions for differentiation toward therapeutically relevant cell types... - The impact of developmental biology on pluripotent stem cell research: successes and challengesJanet Rossant
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
Dev Cell 21:20-3. 2011..However, details of developmental pathways differ even across mammalian species. Full realization of the potential of stem cells will require more direct studies of human or primate developmental biology... - Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cellsPeter J Rugg-Gunn
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
Dev Cell 22:887-901. 2012..These results provide a comprehensive stem cell proteomic resource and enable new approaches to interrogate the mechanisms that regulate cell fate specification... - Disruption of early proximodistal patterning and AVE formation in Apc mutantsClaire Chazaud
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
Development 133:3379-87. 2006..In addition, we found that nuclear beta-catenin and other molecular markers are asymmetrically expressed by 4.5 days... - Live imaging and genetic analysis of mouse notochord formation reveals regional morphogenetic mechanismsYojiro Yamanaka
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
Dev Cell 13:884-96. 2007..Therefore, we propose three distinct regions within the mouse notochord, each with unique morphogenetic origins... - Hedgehog regulates distinct vascular patterning events through VEGF-dependent and -independent mechanismsLeigh Coultas
Developmental and Stem Cell Biology Program, Hospital for Sick Children Research Institute, 555 University Ave, Toronto, Ontario, Canada
Blood 116:653-60. 2010..These findings establish that Hh uses both VEGF- and Notch-dependent and -independent mechanisms to pattern specific events in early blood vascular development... - Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocystDan Strumpf
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Ontario, Canada
Development 132:2093-102. 2005..Thus, Cdx2 is essential for segregation of the ICM and TE lineages at the blastocyst stage by ensuring repression of Oct4 and Nanog in the TE... - Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2Amy Ralston
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, Toronto, ON M5G 1L7, Canada
Development 137:395-403. 2010.... - Vascular endothelial growth factor directly inhibits primitive neural stem cell survival but promotes definitive neural stem cell survivalTamaki Wada
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 3E1
J Neurosci 26:6803-12. 2006..In conclusion, VEGF-A function through Flk1 mediates survival (and not proliferative or fate change) effects on NSCs, specifically... - Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryoAmy Ralston
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, 101 College St, Toronto, ON, Canada
Dev Biol 313:614-29. 2008..Cdx2 therefore appears to act downstream of the first lineage decision, suggesting that processes influencing lineage allocation or morphogenesis may regulate Cdx2 expression along the inside/outside axis of the embryo... - Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetryJun K Takeuchi
Department of Cardiovascular Research, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
Proc Natl Acad Sci U S A 104:846-51. 2007..Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry... - Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathwayClaire Chazaud
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
Dev Cell 10:615-24. 2006..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers... - Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genesBrian Cox
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada
Mol Syst Biol 3:109. 2007..These targets are evidence that Nmyc is a regulator of genes involved in mRNA processing and a repressor of the imprinted gene Igf2r in the developing lung... - Porcupine homolog is required for canonical Wnt signaling and gastrulation in mouse embryosSteffen Biechele
Department of Molecular Genetics, University of Toronto, ON, Canada
Dev Biol 355:275-85. 2011..Taken together, these data confirm the importance of Porcn for Wnt secretion and gastrulation and suggest that disruption of early development underlies the male lethality of human PORCN mutants... - Baf60c is essential for function of BAF chromatin remodelling complexes in heart developmentHeiko Lickert
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
Nature 432:107-12. 2004.... - Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherinRobert Odell Stephenson
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
Development 137:3383-91. 2010..They also show that Cdx2 expression is strongly linked to apical membrane polarization... - Translational analysis of mouse and human placental protein and mRNA reveals distinct molecular pathologies in human preeclampsiaBrian Cox
The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
Mol Cell Proteomics 10:M111.012526. 2011..We conclude that clinically similar preeclampsia patients exhibit divergent placental gene expression profiles thus implicating divergent molecular mechanisms in the origins of this disease... - Foxh1 is essential for development of the anterior heart fieldIngo Von Both
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
Dev Cell 7:331-45. 2004..Thus, Foxh1 and Nkx2-5 functionally interact and are essential for development of the AHF and its derivatives, the RV and OFT, in response to TGFbeta-like signals... - Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profilingThomas Kislinger
Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada
Cell 125:173-86. 2006..This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal... - Comparative systems biology of human and mouse as a tool to guide the modeling of human placental pathologyBrian Cox
The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
Mol Syst Biol 5:279. 2009..The entire data are available as a web-accessible database to guide the informed development of mouse models to study human disease... - Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis developmentPantelis Georgiades
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
Development 133:1059-68. 2006..This study provides new insights about the genetic and cellular basis of the patterning role and development of the early trophoblast... - Lunatic Fringe-mediated Notch signaling is required for lung alveogenesisKeli Xu
Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation... - Zfp206 regulates ES cell gene expression and differentiationWen Zhang
Department of Molecular and Medical Genetics, University of Toronto, 4388 Medical Sciences Building, 1 King s College Circle, Toronto, ON M5S 1A8, Canada
Nucleic Acids Res 34:4780-90. 2006..Together, these data indicate that Zfp206 is a regulator of ES cell differentiation that controls a set of genes expressed very early in development, most of which themselves appear to be regulators... - Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotencyAkitsu Hotta
Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
Nat Methods 6:370-6. 2009..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2... - A novel Phex mutation in a new mouse model of hypophosphatemic ricketsCeleste Owen
Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
J Cell Biochem 113:2432-41. 2012.... - Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryoKashif Ahmed
Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada
PLoS ONE 5:e10531. 2010..Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells... - Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitorsMasatsugu Ema
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
Blood 107:111-7. 2006..Thus, Flk1 expression marks progenitors with broad mesodermal potential but is not absolutely required for the development of all mesodermal lineages in which it is expressed... - Genome-wide analysis of mouse transcripts using exon microarrays and factor graphsBrendan J Frey
Electrical and Computer Engineering, University of Toronto, 10 King s College Rd, Toronto, Ontario M5S 3G4, Canada
Nat Genet 37:991-6. 2005..GenRate also detected tens of thousands of potential new exons and reconciled discrepancies in current cDNA databases by 'stitching' new transcribed regions into previously annotated genes... - Cdx2 is essential for axial elongation in mouse developmentKallayanee Chawengsaksophak
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
Proc Natl Acad Sci U S A 101:7641-5. 2004..The gene appears to be important in the integration of the pathways controlling embryonic axial elongation, and anterior-posterior patterning... - Making the blastocyst: lessons from the mouseKatie Cockburn
Department of Molecular Genetics, University of Toronto, Canada
J Clin Invest 120:995-1003. 2010..A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells... - Activated Fps/Fes partially rescues the in vivo developmental potential of Flk1-deficient vascular progenitor cellsJody J Haigh
Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Ave, Toronto, Ontario, Canada M5G 1X5
Blood 103:912-20. 2004..We have also demonstrated that activated Fps/Fes causes hemangioma formation in vivo, independently of Flk1, as a result of increasing vascular progenitor density... - FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocystYojiro Yamanaka
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
Development 137:715-24. 2010..In conclusion, we propose a model in which stochastic and progressive specification of EPI and PE lineages occurs during maturation of the blastocyst in an FGF/MAP kinase signal-dependent manner... - The role of FGF/Erk signaling in pluripotent cellsFredrik Lanner
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
Development 137:3351-60. 2010..Understanding how extrinsic signals such as FGF regulate different stem cell states will be crucial to harvest the clinical promise of induced pluripotent and embryo-derived stem cells... - Lineage allocation and asymmetries in the early mouse embryoJanet Rossant
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
Philos Trans R Soc Lond B Biol Sci 358:1341-8; discussion 1349. 2003.... - Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryoPeter J Rugg-Gunn
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, ON, Canada
Proc Natl Acad Sci U S A 107:10783-90. 2010..These studies indicate that histone modification mechanisms may differ between early embryo lineages and emphasize the importance of examining in vivo and in vitro progenitor cells... - Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochordOwen J Tamplin
Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
Dev Biol 360:415-25. 2011..The combination of highly specific gene expression profiling and genome-wide ChIP analysis is a powerful means of understanding developmental pathways, even for small cell populations such as the notochord... - Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivativesOwen J Tamplin
Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
BMC Genomics 9:511. 2008..However, the targets of Foxa2 during embryogenesis, and the molecular impact of organizer loss on the gastrula embryo, have not been well defined... - Adhesion is prerequisite, but alone insufficient, to elicit stem cell pluripotencyPhillip Karpowicz
Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada M5S 1A8
J Neurosci 27:5437-47. 2007..These adhesive differences suggest an evolving compartmentalization in multipotent NSCs during development and serve to illustrate the importance of cell-cell association for revealing cellular contribution... - Lineage development and polar asymmetries in the peri-implantation mouse blastocystJanet Rossant
Samuel Lunenfeld Research Institute, Mount Sinal Hospital, Toronto, Ont, Canada
Semin Cell Dev Biol 15:573-81. 2004..The exact relationship between lineage segregation, preimplantation polarities and the postimplantation axes remain to be determined but are now open to molecular and cellular investigation... - Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cellsCheryle A Séguin
The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1L7, Canada
Cell Stem Cell 3:182-95. 2008..These endoderm progenitor cells maintain a stable phenotype through many passages in culture, thereby providing new tools to explore the pathways of endoderm differentiation... - High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivo in miceJohn C Slevin
Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Canada
BMC Dev Biol 6:10. 2006..This method may be useful for determining the developmental effects of targeted genetic or cellular interventions at critical stages of placentation, gastrulation, axis formation, and neural tube closure... - The V-ATPase a3 subunit mutation R740S is dominant negative and results in osteopetrosis in miceNoelle Ochotny
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
J Bone Miner Res 26:1484-93. 2011..These results suggest that the V(0) complex has proton-pumping-independent functions in mammalian cells... - Cell and molecular regulation of the mouse blastocystYojiro Yamanaka
Program of Developmental Biology, Hospital for Sick Children, Toronto, Ontario, Canada
Dev Dyn 235:2301-14. 2006..Together, these mechanisms guide the differentiation of the first lineages of the mouse and thereby set up tissues that will be important for the first steps of embryonic body patterning... - Endothelial cells and VEGF in vascular developmentLeigh Coultas
The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8 Canada
Nature 438:937-45. 2005.... - Three-dimensional analysis of vascular development in the mouse embryoJohnathon R Walls
Mouse Imaging Centre, Hospital for Sick Children, Toronto Centre for Phenogenomics, Toronto, Ontario, Canada
PLoS ONE 3:e2853. 2008..0 and E10.0. This atlas is freely available at http://www.mouseimaging.ca/research/mouse_atlas.html and will serve as a platform to provide insight into normal and abnormal vascular development... - Gene trapping in embryonic stem cellsWilliam L Stanford
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Methods Enzymol 420:136-62. 2006.... - Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryosMarina Gertsenstein
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
PLoS ONE 5:e11260. 2010..Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources... - Trophoblast expression of fms-like tyrosine kinase 1 is not required for the establishment of the maternal-fetal interface in the mouse placentaMasanori Hirashima
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, ON, Canada M5G 1X5
Proc Natl Acad Sci U S A 100:15637-42. 2003..Targeting maternal sFlt1 levels for treatment of preeclampsia may thus be possible without affecting the proper formation of the placenta... - Mouse trophoblast stem cellsJennifer Quinn
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Ontario, Canada
Methods Mol Med 121:125-48. 2006..This chapter will discuss the initial derivation of trophoblast stem cells from the blastocyst stage, maintenance, differentiation, flow cytometry and transfection techniques that can be used with these cells... - Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR proteinAmy P Wong
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
Nat Biotechnol 30:876-82. 2012..Our study provides a method for generating patient-specific airway epithelial cells for disease modeling and in vitro drug testing... - Cytopenia induction by 5-fluorouracil identifies thrombopoietic mutants in sensitized ENU mutagenesis screensNicole M Anderson
Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ont, Canada
Exp Hematol 40:48-60. 2012.... - How signaling promotes stem cell survival: trophoblast stem cells and Shp2Amy Ralston
The Hospital for Sick Children, and Department of Medical Genetics and Microbiology, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
Dev Cell 10:275-6. 2006..These studies provide a novel link between FGF/Erk signaling and cell survival that may be relevant to other stem and progenitor cell niches... - Emerging asymmetry and embryonic patterning in early mouse developmentJanet Rossant
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Ontario M5G 1X5, Canada
Dev Cell 7:155-64. 2004..Whether these asymmetries are causally related to embryonic patterning or are coincidental outcomes of the topology of normal development remains uncertain... - Two mouse mutations mapped to chromosome 11 with differing morphologies but similar progressive inflammatory alopeciaGeoffrey A Wood
Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
Exp Dermatol 14:373-9. 2005..The early onset of the disease, predictable progression, and differences in inflammatory cell localization between the two mutants make these mice particularly useful models for inflammatory hair loss and autoimmune diseases in general... - The genetics of induced pluripotencyAmy Ralston
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
Reproduction 139:35-44. 2010..Knowledge of the requirement for these genes in the embryo and in embryonic stem cells will advance our understanding of how to reverse the developmental clock for therapeutic benefit... - The Crumbs complex couples cell density sensing to Hippo-dependent control of the TGF-β-SMAD pathwayXaralabos Varelas
Center for Systems Biology, Mount Sinai Hospital, Toronto, ON, Canada
Dev Cell 19:831-44. 2010..Accordingly, disruption of the Crumbs complex enhances TGF-β signaling and predisposes cells to TGF-β-mediated epithelial-to-mesenchymal transitions... - Gene-trap expression screening to identify endothelial-specific genesMasanori Hirashima
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
Blood 104:711-8. 2004..Gene-trap expression screening can thus be used to identify early endothelial-specific genes and analyze their function in mice... - Phenotypic annotation of the mouse X chromosomeBrian J Cox
Program in Developmental and Stem Cell Biology, The Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1L7, Canada
Genome Res 20:1154-64. 2010..The entire data set is available via the publicly accessible website (http://xlinkedgenes.ibme.utoronto.ca/)... - The functional landscape of mouse gene expressionWen Zhang
Banting and Best Department of Medical Research, University of Toronto, 1 King s College Circle, Toronto, ON M5S 1A8, Canada
J Biol 3:21. 2004.... - Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous stateFredrik Lanner
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Stem Cells 28:191-200. 2010..We conclude that N-sulfated HS is required for FGF4 signaling to maintain ES cells primed for differentiation in a heterogeneous state. Inhibiting this pathway facilitates a more naïve ground state... - The challenge of regulating rapidly changing science: stem cell legislation in CanadaPeter J Rugg-Gunn
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
Cell Stem Cell 4:285-8. 2009..We encourage scientists and policymakers to collaborate to ensure a clear regulatory framework that accommodates future advances... - Vascular development and patterning: making the right choicesJanet Rossant
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
Curr Opin Genet Dev 13:408-12. 2003.... - Magnetic resonance imaging for detection and analysis of mouse phenotypesBrian J Nieman
Mouse Imaging Centre, Hospital for Sick Children, Toronto, Canada
NMR Biomed 18:447-68. 2005..In particular, the needs for high-throughput imaging and comparative anatomical analyses in large biological studies are described and current efforts at handling these issues are presented... - Cell fate decisions in early blood vessel formationMasatsugu Ema
Samuel Lunenfeld Research Institute and the Department of Molecular and Medical Genetics, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
Trends Cardiovasc Med 13:254-9. 2003.... - Spatial and temporal patterns of ERK signaling during mouse embryogenesisLaura Beth Corson
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
Development 130:4527-37. 2003..These studies identify key domains of sustained ERK signaling in the intact mouse embryo, give significant insight into the regulation of this signaling in vivo and pinpoint regions where downstream target genes can be sought... - Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotypeTilo Kunath
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5
Nat Biotechnol 21:559-61. 2003..Such embryos, in which endogenous p120-Ras GTPase-activating protein (RasGAP), encoded by Rasa1 (also known as RasGAP), was silenced, had the same phenotype as did the previously reported Rasa1 null mutation... - Signaling pathways in vascular developmentJanet Rossant
Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Molecular and Medical Genetics, University of Toronto, 600 University Avenue, Ontario, M5G 1X5, Canada
Annu Rev Cell Dev Biol 18:541-73. 2002..Further understanding of the complexities of the pathways should help refine such strategies... - The Centre for Modeling Human Disease Gene Trap resourceChristine To
Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
Nucleic Acids Res 32:D557-9. 2004..asp). The clones are available as a resource to researchers worldwide to help to functionally annotate the mammalian genome and will serve as a source to test candidate loci identified by phenotype-driven mutagenesis screens... - A novel in vitro model of trophoblast-mediated decidual blood vessel remodelingCaroline Dunk
Departments of Physiology and Fetal and Maternal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Lab Invest 83:1821-8. 2003.... - Molecular profiling of non-small cell lung cancer and correlation with disease-free survivalDennis A Wigle
Thoracic Oncology Site Group, Princess Margaret Hospital, Samuel Lunenfeld Research Institute of Mount Sinai Hospital, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
Cancer Res 62:3005-8. 2002..The results provide evidence that molecular subtyping of NSCLC can identify distinct profiles of gene expression correlating with disease-free survival... - PRISM, a generic large scale proteomic investigation strategy for mammalsThomas Kislinger
Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada
Mol Cell Proteomics 2:96-106. 2003..PRISM therefore offers an effective platform for in-depth investigation of complex mammalian proteomes... - The mouse genome sequence--the end of the tail, or just the beginning?Janet Rossant
Samuel Lunenfeld Research Institute, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
Genome Biol 4:109. 2003..Each illustrates how the availability of the genome sequence will change the way mouse biologists do business in future... - A Gja1 missense mutation in a mouse model of oculodentodigital dysplasiaAnn M Flenniken
Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Development 132:4375-86. 2005..Thus, these mice represent an experimental model with which to explore the clinical manifestations of ODDD and to evaluate potential intervention strategies... - Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiationHitoshi Niwa
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
Cell 123:917-29. 2005..This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development... - Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocystFrançois Gerbe
INSERM, UMR384, Faculte de Medecine, 28 place H Dunant BP38, Clermont Ferrand 63001, Universite d Auvergne, Clermont Fd, France
Dev Biol 313:594-602. 2008..Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps... - Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificityTetsuya S Tanaka
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, 21224 6820, USA
Genome Res 12:1921-8. 2002..We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage... - Remembering Rosa Beddington--a tribute from her friends and colleaguesPaul F Goetinck
Dev Dyn 223:3-11. 2002 - Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouseMasatsugu Ema
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada M5G 1X5
Genes Dev 17:380-93. 2003..We propose a model in which the combinatorial effects of Flk1 and Tal1 act to regulate cell fate choice in early development into hematopoietic, endothelial, and smooth muscle lineages... - A monoclonal mouse?Janet Rossant
Nature 415:967-9. 2002 - Trophoblast-specific gene manipulation using lentivirus-based vectorsPantelis Georgiades
Developmental Genetics and Embryology Research Unit, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus
Biotechniques 42:317-8, 320, 322-5. 2007..This lentivirus lineage-specific infection system thus facilitates both gain and loss of function studies during placental development in the mouse and potentially other mammalian species... - Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH geneJ Henri Bayle
Howard Hughes Medical Institute and the Department Pathology, Beckman Center for Molecular and Genetic Medicine, Stanford University, Stanford, California 94305, USA
J Biol Chem 277:28884-91. 2002..DCoH function as it pertains to HNF1 activity appears to be partially complemented by a newly identified homolog, DCoH2... - Ethics. The ISSCR guidelines for human embryonic stem cell researchGeorge Q Daley
Children's Hospital, Boston, Massachusetts, USA
Science 315:603-4. 2007 - Characterization of human embryonic stem cell lines by the International Stem Cell InitiativeOluseun Adewumi
UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
Nat Biotechnol 25:803-16. 2007..Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected... - The International Stem Cell Initiative: toward benchmarks for human embryonic stem cell researchPeter W Andrews
Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
Nat Biotechnol 23:795-7. 2005 - Transcriptome analysis of mouse stem cells and early embryosAlexei A Sharov
National Institute on Aging, Baltimore, Maryland, USA
PLoS Biol 1:E74. 2003..The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine... - Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genesChiharu Kimura-Yoshida
Department of Molecular Embryology, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka Prefectural Hospital Organization, Murodo cho, Izumi, Osaka 594 1101, Japan
Proc Natl Acad Sci U S A 104:5919-24. 2007..These findings provide profound insights into conserved roles of Foxa2 transcription factors in anterior specification throughout the evolution of the chordate body plan... - Mouse embryonic chimeras: tools for studying mammalian developmentPatrick P L Tam
Embryology Unit, Children s Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia
Development 130:6155-63. 2003..By combining embryonic stem cell technology, molecularly tagged mutations and sensitive cell lineage markers, chimeras can provide invaluable insights into the tissue-specific requirement and the mode of action of many mouse genes... - Control of effector CD8+ T cell function by the transcription factor EomesoderminErika L Pearce
Abramson Family Cancer Research Institute, and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 302:1041-3. 2003..We suggest that Eomesodermin is likely to complement the actions of T-bet and act as a key regulatory gene in the development of cell-mediated immunity... - Connexin31-deficient trophoblast stem cells: a model to analyze the role of gap junction communication in mouse placental developmentMark Kibschull
Institute of Anatomy, University Hospital Duisburg Essen, 45122, Germany
Dev Biol 273:63-75. 2004..We conclude that during trophoblast cell lineage differentiation, the Cx31 gap junction channel is involved in maintaining the proliferative diploid trophoblast cell population... - Stem cells. Setting standards for human embryonic stem cellsAli H Brivanlou
Rockefeller University, New York, NY 10021, USA
Science 300:913-6. 2003 - The mouse embryo autonomously acquires anterior-posterior polarity at implantationKatsuyoshi Takaoka
Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
Dev Cell 10:451-9. 2006..This asymmetry in molecular patterning of the mouse embryo pushes back the origin of the A-P body axis to the peri-implantation stage... - Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocystsTilo Kunath
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada
Development 132:1649-61. 2005..Based on gene expression analysis, chimera studies and imprinted X-inactivation, XEN cell lines are representative of extra-embryonic endoderm and provide a new cell culture model of an early mammalian lineage...