Gail Otulakowski

Summary

Affiliation: The Hospital for Sick Children
Country: Canada

Publications

  1. ncbi request reprint Amiloride-insensitive Na+ and fluid absorption in the mammalian distal lung
    Hugh O'Brodovich
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Department of Pediatrics, University of Toronto, Ontario, Canada M5G 1X8
    Am J Physiol Lung Cell Mol Physiol 294:L401-8. 2008
  2. doi request reprint Global and gene-specific translational regulation in rat lung development
    Gail Otulakowski
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G1X8, Canada
    Am J Respir Cell Mol Biol 40:555-67. 2009
  3. ncbi request reprint Steroid and oxygen effects on eIF4F complex, mTOR, and ENaC translation in fetal lung epithelia
    Gail Otulakowski
    CIHR Group in Lung Development, Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Toronto, ON, Canada
    Am J Respir Cell Mol Biol 37:457-66. 2007
  4. ncbi request reprint Oxygen and glucocorticoids modulate alphaENaC mRNA translation in fetal distal lung epithelium
    Gail Otulakowski
    Programme in Lung Biology Research, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Am J Respir Cell Mol Biol 34:204-12. 2006
  5. doi request reprint Glucocorticoid-mediated repression of REDD1 mRNA expression in rat fetal distal lung epithelial cells
    Gail Otulakowski
    CIHR Group in Lung Development, Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Toronto, Ontario, M5G 1X8, Canada
    Pediatr Res 65:514-9. 2009
  6. ncbi request reprint Differential translational efficiency of ENaC subunits during lung development
    Gail Otulakowski
    CIHR Group in Lung Development, Research Institute of the Hospital for Sick Children, Programme in Lung Biology Research, 555 University Avenue, Toronto, ON, M5G 1X8 Canada
    Am J Respir Cell Mol Biol 30:862-70. 2004
  7. doi request reprint Long-term terbutaline exposure stimulates alpha1-Na+-K+-ATPase expression at posttranscriptional level in rat fetal distal lung epithelial cells
    Muhammad S Rahman
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, 555 Univ Ave, Toronto, Ontario, Canada M5G 1X8
    Am J Physiol Lung Cell Mol Physiol 298:L96-L104. 2010
  8. ncbi request reprint The role of alpha-, beta-, and gamma-ENaC subunits in distal lung epithelial fluid absorption induced by pulmonary edema fluid
    Nael Elias
    Program in Lung Biology, Hospital for Sick Children Research Institute, Toronto, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L537-45. 2007
  9. doi request reprint Pulmonary neuroendocrine cell-secreted factors may alter fetal lung liquid clearance
    Shephali G Gandhi
    CIHR Group in Lung Development, Hospital for Sick Children, Research Institute, Toronto, Ontario, Canada
    Pediatr Res 65:274-8. 2009
  10. doi request reprint Lung-derived soluble mediators are pathogenic in ventilator-induced lung injury
    Thomas Jaecklin
    Programme in Physiology and Experimental Medicine, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Am J Physiol Lung Cell Mol Physiol 300:L648-58. 2011

Collaborators

  • Hugh O'Brodovich
  • Martin Post
  • Ernest Cutz
  • Nael Elias
  • L Jain
  • P M Barker
  • Gerard Curley
  • Douglas Eaton
  • Otto Helve
  • Brian P Kavanagh
  • Doreen Engelberts
  • Vanya Peltekova
  • Thomas Jaecklin
  • Shephali G Gandhi
  • Satoko Uematsu
  • Takehiro Niitsu
  • Bijan Rafii
  • Muhammad S Rahman
  • Nicola Ngiam
  • Wenming Duan
  • Katharine Banasikowska
  • Harry Robert Bremner
  • Ian Copland
  • Shinya Tsuchida
  • Aparna Sarangapani
  • Shephali Gandhi
  • Christine Law
  • Alexandra Garces
  • Xi Juan Chen
  • Don Mahuran
  • Hui Fang Bao
  • Michael S Harris
  • Daniel J Gillie
  • Chris Sulowski
  • Tony Cheung
  • Vicky Hannam
  • Tanya Freywald

Detail Information

Publications21

  1. ncbi request reprint Amiloride-insensitive Na+ and fluid absorption in the mammalian distal lung
    Hugh O'Brodovich
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Department of Pediatrics, University of Toronto, Ontario, Canada M5G 1X8
    Am J Physiol Lung Cell Mol Physiol 294:L401-8. 2008
    ..It also provides new information, using primary cultures of rat fetal distal lung epithelia and alveolar type II cells grown under submersion and air-liquid interface culture conditions, regarding putative blockers of this transport...
  2. doi request reprint Global and gene-specific translational regulation in rat lung development
    Gail Otulakowski
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G1X8, Canada
    Am J Respir Cell Mol Biol 40:555-67. 2009
    ..Translational regulation of IL-18 and protein phosphatase 1 regulatory (inhibitor) subunit 2 is gene-specific, as these changes contrast with the corresponding global changes in polysome abundance...
  3. ncbi request reprint Steroid and oxygen effects on eIF4F complex, mTOR, and ENaC translation in fetal lung epithelia
    Gail Otulakowski
    CIHR Group in Lung Development, Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Toronto, ON, Canada
    Am J Respir Cell Mol Biol 37:457-66. 2007
    ..We speculate that at birth increased Po(2) acts with GC through an mTOR-related pathway to increase alpha-ENaC protein synthesis, thereby promoting lung fluid absorption...
  4. ncbi request reprint Oxygen and glucocorticoids modulate alphaENaC mRNA translation in fetal distal lung epithelium
    Gail Otulakowski
    Programme in Lung Biology Research, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Am J Respir Cell Mol Biol 34:204-12. 2006
    ..These data suggest that postnatal O2 exposure may be important for efficient translation of the alphaENaC mRNA...
  5. doi request reprint Glucocorticoid-mediated repression of REDD1 mRNA expression in rat fetal distal lung epithelial cells
    Gail Otulakowski
    CIHR Group in Lung Development, Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, Toronto, Ontario, M5G 1X8, Canada
    Pediatr Res 65:514-9. 2009
    ..These studies indicate that REDD1 expression in response to hypoxia and DEX is cell-type specific and that physiologically appropriate levels of PO2 should be used when investigating fetal lung development...
  6. ncbi request reprint Differential translational efficiency of ENaC subunits during lung development
    Gail Otulakowski
    CIHR Group in Lung Development, Research Institute of the Hospital for Sick Children, Programme in Lung Biology Research, 555 University Avenue, Toronto, ON, M5G 1X8 Canada
    Am J Respir Cell Mol Biol 30:862-70. 2004
    ..We conclude that there are different translational efficiencies for ENaC subunits and that perinatal processes globally modulate lung mRNA translation...
  7. doi request reprint Long-term terbutaline exposure stimulates alpha1-Na+-K+-ATPase expression at posttranscriptional level in rat fetal distal lung epithelial cells
    Muhammad S Rahman
    Program in Physiology and Experimental Medicine, Hospital for Sick Children Research Institute, 555 Univ Ave, Toronto, Ontario, Canada M5G 1X8
    Am J Physiol Lung Cell Mol Physiol 298:L96-L104. 2010
    ....
  8. ncbi request reprint The role of alpha-, beta-, and gamma-ENaC subunits in distal lung epithelial fluid absorption induced by pulmonary edema fluid
    Nael Elias
    Program in Lung Biology, Hospital for Sick Children Research Institute, Toronto, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L537-45. 2007
    ..01) across all genotype groups. We conclude that beta- and gamma-, but not alpha-, ENaC subunits are essential for EF to exert its maximal effect on net fluid absorption by distal lung epithelia...
  9. doi request reprint Pulmonary neuroendocrine cell-secreted factors may alter fetal lung liquid clearance
    Shephali G Gandhi
    CIHR Group in Lung Development, Hospital for Sick Children, Research Institute, Toronto, Ontario, Canada
    Pediatr Res 65:274-8. 2009
    ..We conclude that at least one, but not all mediators released by pulmonary neuroendocrine cells alter distal lung epithelial ion transport...
  10. doi request reprint Lung-derived soluble mediators are pathogenic in ventilator-induced lung injury
    Thomas Jaecklin
    Programme in Physiology and Experimental Medicine, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Am J Physiol Lung Cell Mol Physiol 300:L648-58. 2011
    ..Although no single class of factor appears solely responsible for the decrease in barrier function, the current data implicate lipid-soluble protein-bound molecules as not just markers but pathogenic mediators in VILI...
  11. doi request reprint Dissociation of inflammatory mediators and function: experimental lung injury in nonpulmonary sepsis
    Satoko Uematsu
    Program in Physiology and Experimental Medicine, Hospital for Sick Children, University of Toronto, Ontario, Canada
    Crit Care Med 41:151-8. 2013
    ..Inflammatory mediators in the plasma or bronchoalveolar fluid are sometimes proposed as biomarkers in ICU patients...
  12. ncbi request reprint Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung
    Shephali G Gandhi
    Canadian Institutes of Health Research Group in Lung Development, University of Toronto, Toronto, Ontario, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L651-9. 2007
    ..EF did increase, by amiloride-sensitive pathways, the alveolar fluid clearance from the lungs of adult rats. We conclude that cardiogenic EF increases Na(+) transport by adult lung epithelia in primary cell culture, in situ and in vivo...
  13. doi request reprint Hypercapnic acidosis in ventilator-induced lung injury
    Vanya Peltekova
    Physiology and Experimental Medicine, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada
    Intensive Care Med 36:869-78. 2010
    ..Ventilator-associated lung injury has a wide spectrum of candidate mechanisms, affording multiple opportunities for intervention such as hypercapnia to exert benefit or harm...
  14. pmc Pulmonary oedema fluid induces non-alpha-ENaC-dependent Na(+) transport and fluid absorption in the distal lung
    Bijan Rafii
    Lung Biology Programme of the Research Institute of The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8
    J Physiol 544:537-48. 2002
    ..Thus proteins or protein-dependent factors within cardiogenic EF induce an alpha-ENaC-independent and amiloride-insensitive apical membrane Na(+) conductance and liquid absorption in the distal lung...
  15. ncbi request reprint Expression of epithelial sodium channel alpha-subunit mRNAs with alternative 5'-untranslated regions in the developing human lung
    Katharine Banasikowska
    Canadian Institutes for Health Research Group in Lung Development, Research Institute of the Hospital for Sick Children, Ontario, Canada M5G 1X8
    Am J Physiol Lung Cell Mol Physiol 287:L608-15. 2004
    ....
  16. doi request reprint Cyclooxygenase inhibition in ventilator-induced lung injury
    Takehiro Niitsu
    Program in Physiology and Experimental Medicine, Hospital for Sick Children, Toronto, Canada
    Anesth Analg 112:143-9. 2011
    ..Adult male rats were anesthetized and randomized with or without nonselective COX inhibition (ibuprofen) and were subjected to injurious mechanical ventilation (positive end-expiratory pressure = 0; peak inspiratory pressure = 21 mm Hg)...
  17. doi request reprint Early growth response-1 worsens ventilator-induced lung injury by up-regulating prostanoid synthesis
    Nicola Ngiam
    Physiology and Experimental Medicine, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Am J Respir Crit Care Med 181:947-56. 2010
    ..We have demonstrated increased expression of the early growth response-1 (Egr1) gene by injurious ventilation, but whether-or how-such up-regulation contributes to injury is unknown...
  18. doi request reprint Do soluble mediators cause ventilator-induced lung injury and multi-organ failure?
    Thomas Jaecklin
    Physiology and Experimental Medicine Program, Hospital for Sick Children, University of Toronto, Toronto, Canada
    Intensive Care Med 36:750-7. 2010
    ..Among the commonly reported classes of ventilation-associated mediators are cytokines, coagulation factors, hormones (e.g., angiotensin-II), lipid-derived mediators and oxidants, yet proof of their pathogenicity is lacking...
  19. ncbi request reprint Promoter analysis of the gene encoding the beta-subunit of the rat amiloride-sensitive epithelial sodium channel
    Harry Robert Bremner
    Canadian Institutes of Health Research Group in Lung Development, Lung Biology Programme, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Am J Physiol Lung Cell Mol Physiol 282:L124-34. 2002
    ..In a comparison of these data with those in published studies on alpha- and gamma-ENaC promoters, the beta- and gamma-subunit promoters appear to be more similar to each other than to the alpha-promoter...
  20. ncbi request reprint Low expression of human epithelial sodium channel in airway epithelium of preterm infants with respiratory distress
    Otto Helve
    Department of Neonatology, Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
    Pediatrics 113:1267-72. 2004
    ..In addition, the effect of systemic dexamethasone therapy on the 3 hENaC subunits was studied in 4 preterm infants who received prolonged assisted ventilation...
  21. ncbi request reprint Thyroid hormone and Na+-K+-ATPase: more than simple transcription
    Gail Otulakowski
    Am J Physiol Lung Cell Mol Physiol 292:L4-5. 2007