James Ellis

Summary

Affiliation: The Hospital for Sick Children
Country: Canada

Publications

  1. ncbi request reprint Retrovirus silencing by an epigenetic TRIM
    James Ellis
    Developmental and Stem Cell Biology Program, SickKids Hospital, Toronto, Ontario, Canada
    Cell 131:13-4. 2007
  2. pmc Initiation of DNA replication at the human beta-globin 3' enhancer
    Alla Buzina
    Developmental Biology Program, Hospital for Sick Children Toronto, Ontario, Canada
    Nucleic Acids Res 33:4412-24. 2005
  3. pmc Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy
    Alla Buzina
    Developmental and Stem Cell Biology Program, SickKids, Toronto, Ontario, Canada
    PLoS Genet 4:e1000051. 2008
  4. pmc X-chromosome inactivation in rett syndrome human induced pluripotent stem cells
    Aaron Y L Cheung
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children Toronto, ON, Canada
    Front Psychiatry 3:24. 2012
  5. pmc MECP2 isoform-specific vectors with regulated expression for Rett syndrome gene therapy
    Mojgan Rastegar
    Developmental and Stem Cell Biology Program, SickKids Hospital, Toronto, Ontario, Canada
    PLoS ONE 4:e6810. 2009
  6. pmc Isolation of MECP2-null Rett Syndrome patient hiPS cells and isogenic controls through X-chromosome inactivation
    Aaron Y L Cheung
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    Hum Mol Genet 20:2103-15. 2011
  7. pmc Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element
    Sylvie Rival-Gervier
    Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Mol Ther 21:1536-50. 2013
  8. pmc Rapid transcriptional pulsing dynamics of high expressing retroviral transgenes in embryonic stem cells
    Mandy Y M Lo
    Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Canada
    PLoS ONE 7:e37130. 2012
  9. pmc Epigenetics of induced pluripotency, the seven-headed dragon
    Ugljesa Djuric
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Canada M5G 1L7
    Stem Cell Res Ther 1:3. 2010
  10. doi request reprint iPSC technology: platform for drug discovery. Point
    J Ellis
    Ontario Human iPS Cell Facility, Toronto, Ontario, Canada
    Clin Pharmacol Ther 89:639-41. 2011

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Retrovirus silencing by an epigenetic TRIM
    James Ellis
    Developmental and Stem Cell Biology Program, SickKids Hospital, Toronto, Ontario, Canada
    Cell 131:13-4. 2007
    ..Epigenetic silencing of retrovirus transcription is accomplished by "writing" a dimethyl mark on lysine 9 of histone H3 that is read by the heterochromatin protein HP1gamma...
  2. pmc Initiation of DNA replication at the human beta-globin 3' enhancer
    Alla Buzina
    Developmental Biology Program, Hospital for Sick Children Toronto, Ontario, Canada
    Nucleic Acids Res 33:4412-24. 2005
    ..We conclude that a mammalian enhancer can cooperate with adjacent sequences to create an efficient replicator module...
  3. pmc Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy
    Alla Buzina
    Developmental and Stem Cell Biology Program, SickKids, Toronto, Ontario, Canada
    PLoS Genet 4:e1000051. 2008
    ..The modified transgenes are the first to transfer intronic elements that potentiate LCR activity and are designed to facilitate correction of hemoglobinopathies using single copy vectors...
  4. pmc X-chromosome inactivation in rett syndrome human induced pluripotent stem cells
    Aaron Y L Cheung
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children Toronto, ON, Canada
    Front Psychiatry 3:24. 2012
    ..Finally, we discuss the relative strengths and weaknesses of post- and pre-XCI hiPSCs in the context of RTT, and other X-linked and autosomal disorders for translational medicine...
  5. pmc MECP2 isoform-specific vectors with regulated expression for Rett syndrome gene therapy
    Mojgan Rastegar
    Developmental and Stem Cell Biology Program, SickKids Hospital, Toronto, Ontario, Canada
    PLoS ONE 4:e6810. 2009
    ..Our objective is to develop viral vectors for MECP2 gene transfer into Neural Stem Cells (NSC) and neurons suitable for gene therapy of Rett Syndrome...
  6. pmc Isolation of MECP2-null Rett Syndrome patient hiPS cells and isogenic controls through X-chromosome inactivation
    Aaron Y L Cheung
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    Hum Mol Genet 20:2103-15. 2011
    ..Analysis of isogenic control and mutant hiPS cell-derived neurons represents a promising source for understanding the pathogenesis of RTT and the role of MECP2 in human neurons...
  7. pmc Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element
    Sylvie Rival-Gervier
    Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Mol Ther 21:1536-50. 2013
    ..We conclude that vectors with both 3'D4Z4 and HS4 insulator elements fully block silencing, and may have unprecedented utility for gene transfer applications that require long-term gene expression in pluripotent stem (PS) cells. ..
  8. pmc Rapid transcriptional pulsing dynamics of high expressing retroviral transgenes in embryonic stem cells
    Mandy Y M Lo
    Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Canada
    PLoS ONE 7:e37130. 2012
    ..These events are directed by a constitutive housekeeping gene promoter and may provide precedence for rapid transcriptional pulsing at endogenous genes in mammalian stem cells...
  9. pmc Epigenetics of induced pluripotency, the seven-headed dragon
    Ugljesa Djuric
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Canada M5G 1L7
    Stem Cell Res Ther 1:3. 2010
    ....
  10. doi request reprint iPSC technology: platform for drug discovery. Point
    J Ellis
    Ontario Human iPS Cell Facility, Toronto, Ontario, Canada
    Clin Pharmacol Ther 89:639-41. 2011
    ..Although challenges remain for efficient differentiation and high-throughput scale-up, we propose that iPSCs have distinct advantages over embryonic stem cells (ESCs) for evaluating drugs by performing clinical trials on a dish...
  11. ncbi request reprint Silencing and variegation of gammaretrovirus and lentivirus vectors
    James Ellis
    Developmental Biology Program, Hospital for Sick Children, Toronto, ON, Canada M5G 1L7
    Hum Gene Ther 16:1241-6. 2005
    ..It will therefore be important to further characterize the mechanisms of silencing, in order to create better gammaretrovirus and lentivirus vectors that consistently express at single copy for gene therapy...
  12. ncbi request reprint Retrovirus silencing and vector design: relevance to normal and cancer stem cells?
    James Ellis
    Developmental Biology Program, Hospital for Sick Children, Toronto, Ontario, Canada
    Curr Gene Ther 5:367-73. 2005
    ..Ultimately, optimized vector designs may prove to be valuable tools for gene therapy of both normal and cancer stem cells...
  13. ncbi request reprint Retrovirus silencing, variegation, extinction, and memory are controlled by a dynamic interplay of multiple epigenetic modifications
    Shuyuan Yao
    Developmental Biology Program, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
    Mol Ther 10:27-36. 2004
    ..A molecular mechanism of competitive H1 and MeCP2 binding may account for this epigenetic interplay, and a model for variegation is discussed...
  14. pmc Retrovirus silencer blocking by the cHS4 insulator is CTCF independent
    Shuyuan Yao
    Developmental Biology Program, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nucleic Acids Res 31:5317-23. 2003
    ....
  15. doi request reprint EOS lentiviral vector selection system for human induced pluripotent stem cells
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, SickKids, Toronto, Ontario, Canada
    Nat Protoc 4:1828-44. 2009
    ..Overall, preparation of the selection system takes 2 weeks, and the generation of human iPS cells takes approximately 2 months...
  16. pmc Constitutive heterochromatin reorganization during somatic cell reprogramming
    Eden Fussner
    Programs in Genetics and Genome Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    EMBO J 30:1778-89. 2011
    ..Thus, constitutive heterochromatin is compacted in partial iPS cells but reorganizes into dispersed 10 nm chromatin fibres as the fully reprogrammed iPS cell state is acquired...
  17. pmc Unexpected acceleration of type 1 diabetes by transgenic expression of B7-H1 in NOD mouse peri-islet glia
    Jason Yantha
    Neuroscience and Mental Health program, Research Institute, The Hospital for Sick Children, Toronto, Ontario, Canada
    Diabetes 59:2588-96. 2010
    ..To distinguish between these alternatives, we sought to suppress pSC autoimmunity by transgenic expression of the negative costimulatory molecule B7-H1 in NOD pSC...
  18. doi request reprint Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
    Nat Methods 6:370-6. 2009
    ..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2...
  19. ncbi request reprint Real-time fluorescence tracking of dynamic transgene variegation in stem cells
    John Ramunas
    Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada
    Mol Ther 15:810-7. 2007
    ..Our approach applies real-time imaging technology to track the long-term dynamics of transgene expression to investigate the timing and expression patterns leading to variegation...
  20. ncbi request reprint LCR-regulated transgene expression levels depend on the Oct-1 site in the AT-rich region of beta -globin intron-2
    Rikki R Bharadwaj
    Developmental Biology Program, Hospital for Sick Children, Toronto, ON, Canada
    Blood 101:1603-10. 2003
    ..In addition, the Oct-1 site in the ATR is required for high-level 5'HS3 beta/gamma-globin transgene expression and should be retained in LCRbeta-globin expression cassettes designed for gene therapy...
  21. pmc Modeling and rescue of the vascular phenotype of Williams-Beuren syndrome in patient induced pluripotent stem cells
    Caroline Kinnear
    Department of Pediatrics, University of Toronto, Toronto, Ontario, Canada
    Stem Cells Transl Med 2:2-15. 2013
    ..The ability of rapamycin to rescue the phenotype provides an attractive therapeutic candidate for patients with WBS and vascular stenoses...
  22. pmc Open and closed domains in the mouse genome are configured as 10-nm chromatin fibres
    Eden Fussner
    Program in Genetics and Genome Biology, Hospital for Sick Children, Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO Rep 13:992-6. 2012
    ..These findings indicate that the 30-nm chromatin model does not reflect the true regulatory structure in vivo...
  23. pmc Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein
    Amy P Wong
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Nat Biotechnol 30:876-82. 2012
    ..Our study provides a method for generating patient-specific airway epithelial cells for disease modeling and in vitro drug testing...
  24. doi request reprint A vertebrate Polycomb response element governs segmentation of the posterior hindbrain
    Angela Sing
    Samuel Lunenfeld Research Institute, Mt Sinai Hospital, Toronto, ON, M5G 1X5, Canada
    Cell 138:885-97. 2009
    ..Thus, we provide evidence that similar to invertebrates, PREs act as entry sites for PcG/trxG chromatin remodeling in vertebrates...
  25. doi request reprint Retroviral vector silencing during iPS cell induction: an epigenetic beacon that signals distinct pluripotent states
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, SickKids Hospital, Toronto, Ontario, Canada
    J Cell Biochem 105:940-8. 2008
    ....
  26. ncbi request reprint Deviation of islet autoreactivity to cryptic epitopes protects NOD mice from diabetes
    Aihua Song
    The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada
    Eur J Immunol 33:546-55. 2003
    ....
  27. ncbi request reprint Transgenic mouse overexpressing syntaxin-1A as a diabetes model
    Patrick P L Lam
    University of Toronto, Room 7226, Medical Science Building, 1 King s College Circle, Toronto, Ontario, Canada
    Diabetes 54:2744-54. 2005
    ....
  28. doi request reprint Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines
    Steven J Kattman
    McEwen Center for Regenerative Medicine, University Health Network, Toronto, Ontario, Canada
    Cell Stem Cell 8:228-40. 2011
    ..Importantly, we found that individual mouse and human pluripotent stem cell lines require optimization of these signaling pathways for efficient cardiac differentiation, illustrating a principle that may well apply in other contexts...
  29. pmc MBNL proteins repress ES-cell-specific alternative splicing and reprogramming
    Hong Han
    Banting and Best Department of Medical Research and Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Nature 498:241-5. 2013
    ....
  30. pmc Over-Expression of Either MECP2_e1 or MECP2_e2 in Neuronally Differentiated Cells Results in Different Patterns of Gene Expression
    Marija Orlic-Milacic
    Molecular Neuropsychiatry and Development Lab, Campbell Family Mental Health Research Institute, The Centre for Addiction and Mental Health, Toronto, Ontario, Canada
    PLoS ONE 9:e91742. 2014
    ....
  31. pmc A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    Nat Chem Biol 7:566-74. 2011
    ..In mouse embryonic stem cells, UNC0638 reactivated G9a-silenced genes and a retroviral reporter gene in a concentration-dependent manner without promoting differentiation...
  32. doi request reprint Personalized Medicine in the Genomics Era: highlights from an international symposium on childhood heart disease
    Natalie Farra
    Department of Molecular Genetics, University of Toronto, Toronto, Canada
    Future Cardiol 8:157-60. 2012
    ..The symposium focused on international research partnerships and application of current state-of-the-art in genomics and stem cell medicine towards personalized healthcare for childhood onset heart disease...
  33. ncbi request reprint eGFP reporter genes silence LCRbeta-globin transgene expression via CpG dinucleotides
    Bruno Dalle
    Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Ther 11:591-9. 2005
    ..We conclude that dmGFP ameliorates silencing, but optimal LCRbeta-globin expression is obtained in the absence of nonmammalian reporters...
  34. ncbi request reprint Enhanced gene expression through temperature profile-induced variations in molecular architecture of thermoresponsive polymer vectors
    Matthieu D Lavigne
    School of Pharmacy and Biomedical Sciences, and Institute of Biomedical and Biomolecular Science, University of Portsmouth, St Michael s Building, White Swan Road, Portsmouth PO1 2DT, UK
    J Gene Med 9:44-54. 2007
    ..g. the chain-extended coil to chain-collapsed globule phase transition that occurs at a lower critical solution temperature (LCST), in order to transport nucleic acid in one polymer state and release it in another...
  35. pmc Nuclear matrix association of the human beta-globin locus utilizing a novel approach to quantitative real-time PCR
    G Charles Ostermeier
    Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA
    Nucleic Acids Res 31:3257-66. 2003
    ..These results provide the first evidence that nuclear matrix association dynamically mediates the looping of the beta-globin locus to achieve transcriptional control...
  36. ncbi request reprint "Agouti NOD": identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells
    Jing Chen
    The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609 1500, USA
    Mamm Genome 16:775-83. 2005
    ..CBALs- Tyr(+)/Lt blastocysts produced approximately 50% live-born mice, of which approximately 11% were chimeric. Presumably because of high genomic instability, no germline transmission was observed...