R W Stokes
- Two distinct receptors mediate nonopsonic phagocytosis of different strains of Pseudomonas aeruginosaJ P Heale
Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
J Infect Dis 183:1214-20. 2001..The impact of lung infection with P. aeruginosa may be determined, in part, by the phagocytic receptor that mediates ingestion...
- Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficientR W Stokes
Division of Infectious and Immunological Diseases, British Columbia s Children s Hospital, Vancouver, Canada
J Immunol 160:5514-21. 1998..The receptor mediating nonopsonic binding of M. tuberculosis to differentiated alveolar Mphi was not unequivocally identified in this study, but could also be CR3...
- The glycan-rich outer layer of the cell wall of Mycobacterium tuberculosis acts as an antiphagocytic capsule limiting the association of the bacterium with macrophagesRichard W Stokes
Department of Paediatrics, University of British Columbia, Vancouver, British Columbia, Canada
Infect Immun 72:5676-86. 2004..tuberculosis, like many other microbial pathogens, has an antiphagocytic capsule that limits and controls the interaction of the bacterium with macrophages...
- Effect of mycobacterial phospholipids on interaction of Mycobacterium tuberculosis with macrophagesL M Thorson
Division of Infectious and Immunological Diseases, British Columbia's Children's Hospital, University of British Columbia, Vancouver, British Columbia, Canada
Infect Immun 69:2172-9. 2001..We have also shown that the ability of macrophages to interact with mycobacteria is greatly affected by an as yet unidentified product from the interaction of chloroform and polypropylene tubes...
- Utilization of CD11b knockout mice to characterize the role of complement receptor 3 (CR3, CD11b/CD18) in the growth of Mycobacterium tuberculosis in macrophagesM D Melo
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada
Cell Immunol 205:13-23. 2000..However, whether a mycobacterium will gain access to the intracellular environment, and the type of Mφ that the bacterium enters, is significantly affected by the presence or absence of CR3...
- Mycobacterium tuberculosis efpA encodes an efflux protein of the QacA transporter familyJ L Doran
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
Clin Diagn Lab Immunol 4:23-32. 1997..However, efpA was not present in 17 other opportunistically pathogenic or nonpathogenic mycobacterial species...
- CD43 is required for optimal growth inhibition of Mycobacterium tuberculosis in macrophages and in miceApril K Randhawa
Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
J Immunol 175:1805-12. 2005..tuberculosis results in an increased bacterial load during both the acute and chronic stages of infection and more rapid development of granulomas, with greater lung involvement and distinctive cellularity...
- Selection of transposon mutants of Mycobacterium tuberculosis with increased macrophage infectivity identifies fadD23 to be involved in sulfolipid production and association with macrophagesJennifer Lynett
Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V5Z 4H4, Canada
Microbiology 153:3133-40. 2007..Analysis of the cell envelope constituents of the Tn : : fadD23 strain revealed a lack of sulfolipid production which was restored following complementation with the wild-type gene...
- Absence of complement receptor 3 results in reduced binding and ingestion of Mycobacterium tuberculosis but has no significant effect on the induction of reactive oxygen and nitrogen intermediates or on the survival of the bacteria in resident and interfeAmanda W J Rooyakkers
Department of Paediatrics, University of British Columbia, Vancouver, BC, Canada
Microb Pathog 39:57-67. 2005..However, the presence of CR3 was not critical in any of these mechanisms. Furthermore, we demonstrate that the control of intracellular growth of Mtb in IFN-gamma activated Mphis is not mediated by a direct effect of RNI...
- CD43 controls the intracellular growth of Mycobacterium tuberculosis through the induction of TNF-alpha-mediated apoptosisApril K Randhawa
Department of Medicine, University of British Columbia, Vancouver, BC, Canada
Cell Microbiol 10:2105-17. 2008..We propose that CD43 is involved in the inflammatory response to M. tuberculosis and, through the induction of pro-inflammatory mediators, can regulate apoptosis to control intracellular growth of the bacterium...
- Single mucosal, but not parenteral, immunization with recombinant adenoviral-based vaccine provides potent protection from pulmonary tuberculosisJun Wang
Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Centre for Gene Therapeutics, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
J Immunol 173:6357-65. 2004..Our study also lends strong evidence that respiratory mucosal vaccination is critically advantageous over systemic routes of vaccination against TB...
- The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamate/glutamine levels and is important for growth in vivoSiobhan Cowley
Department of Medicine, Division of Infectious Diseases, University of British Columbia, 2733 Heather St, Vancouver, British Columbia, Canada V5Z 3J5
Mol Microbiol 52:1691-702. 2004..These data support our hypothesis that PknG mediates the transfer of signals sensing nutritional stress in M. tuberculosis and translates them into metabolic adaptation...
- Two-dimensional bacterial genome display: a method for the genomic analysis of mycobacteriaEdith M Dullaghan
The Division of Infectious and Immunological Diseases, British Columbia s Children s Hospital, University of British Columbia, Vancouver, Canada
Microbiology 148:3111-7. 2002..That 2DBGD can be used to identify differences resulting from either insertional mutagenesis using a gentamicin-resistance gene or from a frameshift mutation is demonstrated...
- Two-dimensional display and whole genome comparison of bacterial pathogen genomes of high G+C DNA contentChad A Malloff
Department of Pathology, University of British Columbia, Canada
Gene 293:205-11. 2002....
- Characterization of genes differentially expressed within macrophages by virulent and attenuated Mycobacterium tuberculosis identifies candidate genes involved in intracellular growthAlice H Li
Department of Pathology and Laboratory Medicine, University of British Columbia, 950 West 28th Avenue, Vancouver, BC, Canada
Microbiology 154:2291-303. 2008..Lastly, treatment of intracellular bacteria with a putative inhibitor of fumarate reductase resulted in a significant reduction of bacterial growth...