Affiliation: Simon Fraser University
Harden N, Ricos M, Ong Y, Chia W, Lim L. Participation of small GTPases in dorsal closure of the Drosophila embryo: distinct roles for Rho subfamily proteins in epithelial morphogenesis. J Cell Sci. 1999;112 ( Pt 3):273-84 pubmed
..Rather, our results suggest that while all &Rgr; subfamily p21s tested are required for dorsal closure, they act largely in parallel. ..
Wang S, Tsai A, Wang M, Yoo S, Kim H, Yoo B, et al
. Phospho-regulated Drosophila adducin is a determinant of synaptic plasticity in a complex with Dlg and PIP2 at the larval neuromuscular junction. Biol Open. 2014;3:1196-206 pubmed publisher
..These results are further evidence that Hts is a signaling-responsive regulator of synaptic plasticity in Drosophila. ..
Vlachos S, Jangam S, Conder R, Chou M, Nystul T, Harden N. A Pak-regulated cell intercalation event leading to a novel radial cell polarity is involved in positioning of the follicle stem cell niche in the Drosophila ovary. Development. 2015;142:82-91 pubmed publisher
..The wide basal stalk in Pak mutants enables the formation of extra FSC niches which are mispositioned and yet functional, indicating that the FSC niche can be established in diverse locations. ..
Wang S, Yoo S, Kim H, Wang M, Zheng C, Parkhouse W, et al
. Detection of in situ protein-protein complexes at the Drosophila larval neuromuscular junction using proximity ligation assay. J Vis Exp. 2015;:52139 pubmed publisher
..The technique is performed on larval body wall muscle preparations to show that a complex between Dlg and Hts does indeed exist at the postsynaptic region of NMJs. ..
Harden N, Ricos M, Yee K, Sanny J, Langmann C, Yu H, et al
. Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila. J Cell Sci. 2002;115:2119-29 pubmed
..We propose that amnioserosa morphogenesis is a useful system for studying the regulation of epithelial morphogenesis by Drac1. ..
Harden N. Cell biology. Of grainy heads and broken skins. Science. 2005;308:364-5 pubmed
Harden N, Wang S, Krieger C. Making the connection - shared molecular machinery and evolutionary links underlie the formation and plasticity of occluding junctions and synapses. J Cell Sci. 2016;129:3067-76 pubmed publisher
Harden N. New Insights from Drosophila into the Regulation of EGFR Signaling. Methods Mol Biol. 2017;1652:37-42 pubmed publisher
..Recent studies in the fly have led to some surprising results, identifying regulators of Egfr acting in unexpected ways. ..
Reed B, Harden N. Studying Nonproliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo. Methods Mol Biol. 2017;1652:229-256 pubmed publisher
..Although this "tool kit" is designed for looking at Egfr, it can be readily adapted to look at the participation of any signaling molecule in dorsal closure. ..