RAFICK PIERRE SEKALY

Summary

Country: Canada

Publications

  1. ncbi Support for the RV144 HIV vaccine trial
    Robert Belshe
    Science 305:177-80; author reply 177-80. 2004
  2. doi Transcription factor FOXO3a controls the persistence of memory CD4(+) T cells during HIV infection
    Julien van Grevenynghe
    Laboratoire d Immunologie, Centre de Recherche, Hopital Saint Luc, Centre Hospitalier de l Universite de Montreal, 264 Boulevard Rene Levesque Est, Montreal, Quebec H2X 1P1, Canada
    Nat Med 14:266-74. 2008
  3. pmc Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses
    Denis Gaucher
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal CR CHUM Saint Luc, Montreal, Quebec, Canada
    J Exp Med 205:3119-31. 2008
  4. pmc HIV-1 viremia prevents the establishment of interleukin 2-producing HIV-specific memory CD4+ T cells endowed with proliferative capacity
    Souheil Antoine Younes
    Departement de Microbiologie et Immunologie, Universite de Montreal, 2900 Edouard Montpetit Boulevard, Montreal H3T 1J4, Canada
    J Exp Med 198:1909-22. 2003
  5. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  6. pmc FuGEFlow: data model and markup language for flow cytometry
    Yu Qian
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    BMC Bioinformatics 10:184. 2009
  7. pmc The failed HIV Merck vaccine study: a step back or a launching point for future vaccine development?
    RAFICK PIERRE SEKALY
    Universite de Montreal, CR CHUM, Institut National de la Santé et de la Recherche Médicale U743, Montreal, Québec H2X1P1, Canada
    J Exp Med 205:7-12. 2008
  8. pmc Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells
    Catherine Riou
    Laboratoire d Immunologie, Centre de Recherche, Hôpital Saint Luc du Centre Hospitalier de l Université de Montréal CHUM, Montreal, Quebec H2X 1P1, Canada
    J Exp Med 204:79-91. 2007
  9. ncbi The selective increase in caspase-3 expression in effector but not memory T cells allows susceptibility to apoptosis
    Laurent Sabbagh
    Laboratory of Immunology, Universite de Montreal, Montreal, Canada
    J Immunol 173:5425-33. 2004
  10. ncbi Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection
    Elias A Said
    Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Hôpital St Luc, Montreal, Quebec, Canada
    Nat Med 16:452-9. 2010

Research Grants

  1. Novel Concepts for the Eradication of HIV
    RAFICK PIERRE SEKALY; Fiscal Year: 2009
  2. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2006
  3. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2004
  4. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2005

Detail Information

Publications63

  1. ncbi Support for the RV144 HIV vaccine trial
    Robert Belshe
    Science 305:177-80; author reply 177-80. 2004
  2. doi Transcription factor FOXO3a controls the persistence of memory CD4(+) T cells during HIV infection
    Julien van Grevenynghe
    Laboratoire d Immunologie, Centre de Recherche, Hopital Saint Luc, Centre Hospitalier de l Universite de Montreal, 264 Boulevard Rene Levesque Est, Montreal, Quebec H2X 1P1, Canada
    Nat Med 14:266-74. 2008
    ..The crucial role of FOXO3a in the survival of memory cells will help shed light on the underlying immunological mechanisms that control viral replication in EC subjects...
  3. pmc Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses
    Denis Gaucher
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal CR CHUM Saint Luc, Montreal, Quebec, Canada
    J Exp Med 205:3119-31. 2008
    ....
  4. pmc HIV-1 viremia prevents the establishment of interleukin 2-producing HIV-specific memory CD4+ T cells endowed with proliferative capacity
    Souheil Antoine Younes
    Departement de Microbiologie et Immunologie, Universite de Montreal, 2900 Edouard Montpetit Boulevard, Montreal H3T 1J4, Canada
    J Exp Med 198:1909-22. 2003
    ..Our data favor a model whereby competent HIV-specific Tcm continuously arise in small numbers but under persistent antigenemia are rapidly induced to differentiate into IFN-gamma only-producing cells that lack self-renewal capacity...
  5. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  6. pmc FuGEFlow: data model and markup language for flow cytometry
    Yu Qian
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    BMC Bioinformatics 10:184. 2009
    ..We have extended FuGE object model to accommodate flow cytometry data and metadata...
  7. pmc The failed HIV Merck vaccine study: a step back or a launching point for future vaccine development?
    RAFICK PIERRE SEKALY
    Universite de Montreal, CR CHUM, Institut National de la Santé et de la Recherche Médicale U743, Montreal, Québec H2X1P1, Canada
    J Exp Med 205:7-12. 2008
    ....
  8. pmc Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells
    Catherine Riou
    Laboratoire d Immunologie, Centre de Recherche, Hôpital Saint Luc du Centre Hospitalier de l Université de Montréal CHUM, Montreal, Quebec H2X 1P1, Canada
    J Exp Med 204:79-91. 2007
    ..Our results provide, for the first time in humans, an insight into molecular mechanisms that could be responsible for the longevity and persistence of CD4+ TCM...
  9. ncbi The selective increase in caspase-3 expression in effector but not memory T cells allows susceptibility to apoptosis
    Laurent Sabbagh
    Laboratory of Immunology, Universite de Montreal, Montreal, Canada
    J Immunol 173:5425-33. 2004
    ..These findings indicate that the selective up-regulation of caspase-3 transcription is required to maintain the cytoplasmic levels of this protease, which control AICD and T cell homeostasis...
  10. ncbi Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection
    Elias A Said
    Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Hôpital St Luc, Montreal, Quebec, Canada
    Nat Med 16:452-9. 2010
    ..We describe a new function for PD-1 whereby microbial products inhibit T cell expansion and function by upregulating PD-1 levels and IL-10 production by monocytes after binding of PD-1 by PD-L1...
  11. ncbi Generation and maintenance of human memory cells during viral infection
    Rabih Halwani
    Laboratoire d Immunologie, Centre de Recherche du CHUM, Campus St Luc, Pavillon Edouard Asselin, 264 Boulevard Rene Levesque Est, Montreal, Quebec, H3C 3J7, Canada
    Springer Semin Immunopathol 28:197-208. 2006
    ....
  12. ncbi Slow disease progression and robust therapy-mediated CD4+ T-cell recovery are associated with efficient thymopoiesis during HIV-1 infection
    Marie Lise Dion
    Laboratoire d Immunologie, Centre de Recherches du Centre Hospitalier de l Université de Montréal CR CHUM, Hopital Saint Luc, Montreal, QC, Canada
    Blood 109:2912-20. 2007
    ..Overall, we demonstrate that efficient thymopoiesis is key in the natural maintenance and in therapy-mediated recovery of naive and total CD4+ T cells...
  13. ncbi Upregulation of PD-1 expression on HIV-specific CD8+ T cells leads to reversible immune dysfunction
    Lydie Trautmann
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal CR CHUM Saint Luc, 264 Rene Levesque Est, Montreal, Québec H2X1P1, Canada
    Nat Med 12:1198-202. 2006
    ..The accumulation of HIV-specific dysfunctional CD8(+) T cells in the infected host could prevent the renewal of a functionally competent HIV-specific CD8(+) repertoire...
  14. ncbi Reversible blockade of thymic output: an inherent part of TLR ligand-mediated immune response
    Thomas Démoulins
    Laboratoire d Immunologie, Centre de Recherches du Centre Hospitalier de l Université de Montréal, Saint Luc, Canada
    J Immunol 181:6757-69. 2008
    ....
  15. doi Dendritic cell inhibition is connected to exhaustion of CD8+ T cell polyfunctionality during chronic hepatitis C virus infection
    Ian Gaël Rodrigue-Gervais
    Departement de Microbiologie et Immunologie, Centre de Recherche du Centre Hospitalier de Université de Montréal, Montreal, Quebec, Canada
    J Immunol 184:3134-44. 2010
    ..Our findings identify reversible virus evasion of DC-mediated innate immunity as an additional important factor that impacts the severity of polyfunctional CD8(+) T cell exhaustion during a chronic viral infection...
  16. pmc CD160 and PD-1 co-expression on HIV-specific CD8 T cells defines a subset with advanced dysfunction
    Yoav Peretz
    Caprion ImmuneCarta Services, Montreal, Quebec, Canada
    PLoS Pathog 8:e1002840. 2012
    ....
  17. ncbi Peripheral blood CCR4+CCR6+ and CXCR3+CCR6+CD4+ T cells are highly permissive to HIV-1 infection
    Annie Gosselin
    Department of Microbiology and Immunology, Universite de Montreal, Montreal, Quebec, Canada
    J Immunol 184:1604-16. 2010
    ..It is necessary that new therapeutic strategies against HIV interfere with viral replication/persistence in discrete CCR6(+) T cell subsets...
  18. pmc Down-regulation of CTLA-4 by HIV-1 Nef protein
    Mohamed El-Far
    Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Hôpital St Luc, Montreal, Quebec, Canada
    PLoS ONE 8:e54295. 2013
    ..Down-regulation of CTLA-4 may contribute to the mechanisms by which HIV-1 sustains T cell activation, a critical step in viral replication and dissemination...
  19. ncbi HIV infection rapidly induces and maintains a substantial suppression of thymocyte proliferation
    Marie Lise Dion
    Laboratoire d Immunologie, Centre de Recherches du CHUM, Hotel Dieu, Montreal, Quebec H2X 1P1, Canada
    Immunity 21:757-68. 2004
    ..Our findings reveal a substantial reduction in intrathymic proliferation. The analysis also indicates the existence of a compensatory mechanism acting to sustain the numbers of recent thymic emigrants (RTEs) in the periphery...
  20. ncbi T-cell exhaustion in HIV infection
    Mohamed El-Far
    Centre de Recherche du CHUM, Hopital Saint Luc, 264, René Lévesque Est, Bureau 1317, Montreal, Quebec, H2X 1P1, Canada
    Curr HIV/AIDS Rep 5:13-9. 2008
    ..In this review, we examine the molecular mechanisms involved in such T-cell dysfunction. Better understanding of these mechanisms will assist in the development of novel therapies to prevent the immune damage mediated by HIV infection...
  21. ncbi [mRNA-transfected dendritic cells: a promising strategy in immunotherapy]
    Karine Maisnier-Patin
    Laboratoire d Immunologie, Centre de Recherche du CHUM Saint Luc, Montreal, Quebec, Canada
    Med Sci (Paris) 23:279-84. 2007
    ..In this review, we report several advances made in the field of anti-tumoral and anti-HIV immunotherapy using mRNA-transfected dendritic cells-based approaches...
  22. pmc Autologous HIV-1 clade-B Nef peptides elicit increased frequency, breadth and function of CD8+ T-cells compared to consensus peptides
    Mehrnoosh Doroudchi
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal CR CHUM Saint Luc, Montreal, Quebec, Canada
    PLoS ONE 7:e49562. 2012
    ..To determine the function and phenotype of CD8(+) T-cells targeting consensus and autologous sequences of entire HIV-1 Nef protein...
  23. pmc Programmed death-1 is a marker for abnormal distribution of naive/memory T cell subsets in HIV-1 infection
    Gaelle Breton
    Laboratoire d Immunologie, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Saint Luc, Montreal, Quebec H2X 1P1, Canada
    J Immunol 191:2194-204. 2013
    ..Our findings identify PD-1 as a marker for aberrant distribution of T cell subsets in HIV-1 infection. ..
  24. ncbi Caspase-3 is a component of Fas death-inducing signaling complex in lipid rafts and its activity is required for complete caspase-8 activation during Fas-mediated cell death
    Salah M Aouad
    Departement de Microbiologie et Immunologie, Universite de Montreal, Montreal, Quebec, Canada
    J Immunol 172:2316-23. 2004
    ..Overall, these findings support a model whereby caspase-3 is a component of the death-inducing signaling complex located in lipid rafts, and as such, is involved in the amplification of caspase-8 activity by the mitochondrion...
  25. ncbi MMTV Superantigens Coerce an Unconventional Topology between the TCR and MHC Class II
    Jean Simon Fortin
    Laboratoire d Immunologie Moléculaire, Departement de Microbiologie, Infectiologie et Immunologie, Universite de Montreal, Montreal, Quebec HC3 3J7, Canada
    J Immunol 192:1896-906. 2014
    ..Our findings highlight the plasticity of the TCR CDRs. ..
  26. ncbi Evidence for adequate thymic function but impaired naive T-cell survival following allogeneic hematopoietic stem cell transplantation in the absence of chronic graft-versus-host disease
    Jean Francois Poulin
    Laboratoire d Immunologie, Departement de Microbiologie et Immunologie, Universite de Montreal, C P 6128, Succ Centre Ville, Montreal QC H3C 3J7, Canada
    Blood 102:4600-7. 2003
    ....
  27. ncbi The duration of exposure to HIV modulates the breadth and the magnitude of HIV-specific memory CD4+ T cells
    Souheil Antoine Younes
    Laboratoire d Immunologie, Departement de Microbiologie et Immunologie, Universite de Montreal, Montreal, Quebec, Canada
    J Immunol 178:788-97. 2007
    ....
  28. ncbi TLR Ligand-Induced Type I IFNs Affect Thymopoiesis
    Marie Laurence Baron
    Laboratoire d Immunologie, Immunologie Humaine, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Hopital Saint Luc, Montreal, Quebec, Canada
    J Immunol 180:7134-46. 2008
    ..Because TLR ligands are widely used as vaccine adjuvants, their immunomodulatory actions mediated mainly by IFN-alpha suggested by our results should be taken in consideration...
  29. pmc HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation
    Nicolas Chomont
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal Saint Luc, Montreal, Quebec, Canada
    Nat Med 15:893-900. 2009
    ....
  30. ncbi p56Lck tyrosine kinase enhances the assembly of death-inducing signaling complex during Fas-mediated apoptosis
    Ehssan Sharif-Askari
    Laboratoire d Immunologie, Centre de Recherche CHUM Saint Luc, Montréal H2X 1P1, Canada
    J Biol Chem 282:36048-56. 2007
    ..Our results support a novel model whereby sensitivity to apoptosis is regulated through quantitative changes in the stoichiometry of DISC components triggered by p56(Lck) activation and localization...
  31. pmc Immunologic activity and safety of autologous HIV RNA-electroporated dendritic cells in HIV-1 infected patients receiving antiretroviral therapy
    Jean Pierre Routy
    Immunodeficiency Service and Division of Hematology, McGill University Health Centre, McGill University, Montreal, QC, Canada
    Clin Immunol 134:140-7. 2010
    ..No evidence of autoimmunity, changes in viral load, or significant changes in absolute CD4(+) and CD8(+) T cell counts were observed. This pilot study supports the further clinical investigation of AGS-004...
  32. doi Relative contribution of HIV-specific functional lymphocyte subsets restricted by protective and non-protective HLA alleles
    Yoav Peretz
    National Immune Monitoring Laboratory NIML, Genome Quebec, Montreal, Quebec, Canada
    Viral Immunol 24:189-98. 2011
    ..HLA haplotypes influence the relative functional composition of HIV-specific responses...
  33. ncbi The MHC class II beta chain cytoplasmic tail overcomes the invariant chain p35-encoded endoplasmic reticulum retention signal
    Hayssam Khalil
    Laboratoire d Immunologie Moléculaire, Departement de Microbiologie et Immunologie, Faculte de Medecine, Universite de Montreal, CP 6128 Succursale Centre Ville, Montreal, Quebec H3C 3J7, Canada
    Int Immunol 15:1249-63. 2003
    ..Altogether, these results further dissect the masking of di-basic retention signals, and emphasize the interplay between class II molecules and Ii for the transport of the complex to the endocytic pathway...
  34. doi HIV Gag p24 specific responses secreting IFN-gamma and/or IL-2 in treatment-naïve individuals in acute infection early disease (AIED) are associated with low viral load
    Michel L Ndongala
    Research Institute of the McGill University Health Center, Montreal General Hospital, Quebec, Canada
    Clin Immunol 131:277-87. 2009
    ..Therefore the early appearance of broader and more intense Gag-p24-specific responses may be a determinant of subsequent VL...
  35. doi CD4 T cell nadir independently predicts the magnitude of the HIV reservoir after prolonged suppressive antiretroviral therapy
    Mohamed Rachid Boulassel
    Division of Hematology, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada
    J Clin Virol 53:29-32. 2012
    ..However, the relevance of reservoir level while on efficient antiretroviral therapy (ART) is still unknown...
  36. pmc Persistence and fitness of multidrug-resistant human immunodeficiency virus type 1 acquired in primary infection
    Bluma G Brenner
    McGill University AIDS Centre, Jewish General Hospital, Montreal, Quebec, Canada
    J Virol 76:1753-61. 2002
    ..These results indicate that MDR viruses that are generated following PHI can establish persistent infections as dominant quasispecies despite their impaired replicative competence...
  37. pmc HTLV-1 evades type I interferon antiviral signaling by inducing the suppressor of cytokine signaling 1 (SOCS1)
    Stephanie Oliere
    Molecular Oncology Group, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada
    PLoS Pathog 6:e1001177. 2010
    ..These observations identify a novel SOCS1 driven mechanism of evasion of the type I IFN antiviral response against HTLV-1...
  38. ncbi Why T cells of thymic versus extrathymic origin are functionally different
    Marie Eve Blais
    Institute for Research in Immunology and Cancer, and Department of Medicine, University of Montreal, Station Centre Ville, Montreal, Quebec, Canada
    J Immunol 180:2299-312. 2008
    ....
  39. ncbi The magnitude of thymic output is genetically determined through controlled intrathymic precursor T cell proliferation
    Gaël Dulude
    Laboratoire d Immunologie, Centre de Recherches du Centre Hospitalier de l Université Montréal, Saint Luc, Montreal, Quebec, Canada
    J Immunol 181:7818-24. 2008
    ..Our results provide the first demonstration of the genetic regulation of thymic output...
  40. ncbi [Inhibition of the PD-1 pathway restores the effector function of HIV-specific T cells]
    Lydie Trautmann
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal Saint Luc, INSERM U743, 264 Boulevard Rene Levesque Est, Montréal Québec H2X 1P1, Canada
    Med Sci (Paris) 23:24-5. 2007
  41. ncbi Selective up-regulation of caspase-3 gene expression following TCR engagement
    Laurent Sabbagh
    Laboratoire d Immunologie, Centre de Recherche du CHUM, Campus St Luc, Pavillon Edouard Asselin, 264 Boul Rene Levesque Est 1307D, Montreal, Que, Canada H2X 1P1
    Mol Immunol 42:1345-54. 2005
    ..Together, these results show that TCR activation triggers the selective increase in caspase-3 mRNA levels, independently of caspase activity and the induction of apoptosis...
  42. ncbi CD4 dimers constitute the functional component required for T cell activation
    Maria Cristina Moldovan
    Laboratoire d Immunologie, Institut de Recherches Cliniques de Montreal, Quebec, Canada
    J Immunol 169:6261-8. 2002
    ..More importantly, we demonstrate that dimer formation is essential for the coligand and coreceptor functions of CD4 in T cell activation. These data strongly suggest that CD4 dimerization is necessary for helper T cell function...
  43. ncbi Therapeutic vaccination with simian immunodeficiency virus (SIV)-DNA + IL-12 or IL-15 induces distinct CD8 memory subsets in SIV-infected macaques
    Rabih Halwani
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal Saint Luc, Montreal, Quebec, Canada
    J Immunol 180:7969-79. 2008
    ..These results indicate that plasmid IL-12 and IL-15 cytokines represent a significant addition to enhance the ability of therapeutic DNA vaccines to induce better immunity...
  44. doi A conserved polylysine motif in CD86 cytoplasmic tail is necessary for cytoskeletal association and effective co-stimulation
    Tanya Girard
    Department of Microbiology and Immunology, McGill University, Montreal, Canada
    Biochem Biophys Res Commun 423:301-7. 2012
    ..Altogether, our results reveal the critical importance of the cytoskeleton-dependent CD86 polarization to the IS and more specifically the K4 motif for effective co-signaling...
  45. pmc Clonotype and repertoire changes drive the functional improvement of HIV-specific CD8 T cell populations under conditions of limited antigenic stimulation
    Loury Janbazian
    Laboratory of Immunology, Department of Microbiology and Immunology, University of Montreal, Montreal, Quebec H2X 1P1, Canada
    J Immunol 188:1156-67. 2012
    ....
  46. ncbi Th17 lineage commitment and HIV-1 pathogenesis
    Petronela Ancuta
    Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Saint Luc Hospital, Pavillon Edouard Asselin, 264 Blvd René Lévesque East, Montreal, Quebec, Canada
    Curr Opin HIV AIDS 5:158-65. 2010
    ....
  47. pmc Cellular and molecular mechanisms of memory T-cell survival
    Andre Tanel
    Laboratoire d Immunologie, Centre de Recherche du Centre Hospitalier de l Université de Montréal CR CHUM Saint Luc, 264 Rene Levesque Est, Montreal, Quebec H2X 1P1, Canada
    Expert Rev Vaccines 8:299-312. 2009
    ....
  48. pmc Prediction of graft-versus-host disease in humans by donor gene-expression profiling
    Chantal Baron
    Institute of Research in Immunology and Cancer, University of Montreal, Montreal, Quebec, Canada
    PLoS Med 4:e23. 2007
    ..Therefore, we tested the hypothesis that some donors may be "stronger alloresponders" than others, and consequently more likely to elicit GVHD...
  49. ncbi Estimating thymic function through quantification of T-cell receptor excision circles
    Marie Lise Dion
    Laboratoire d Immunologie, Centre de Recherches du CHUM, Montreal, Canada
    Methods Mol Biol 380:197-213. 2007
    ....
  50. doi Poly (I:C) induced immune response in lymphoid tissues involves three sequential waves of type I IFN expression
    Thomas Démoulins
    Laboratoire d Immunologie, Centre de Recherche du CHUM, Hopital Saint Luc, Montreal, Quebec, Canada
    Virology 386:225-36. 2009
    ..This IFN-HTA strategy can serve as a useful tool to qualify and quantify the expression of various IFN-alpha subtypes under distinct immune responses and thus provides a first step in evaluating their function...
  51. ncbi Triggering of T cell activation via CD4 dimers
    Maria Cristina Moldovan
    Department of Microbiology and Immunology, McGill University, Montreal, Canada
    J Immunol 176:5438-45. 2006
    ..This supports a role for CD4 dimerization in maintaining active signaling machinery. We suggest that modulation of the dimer/monomer ratio may permit tuning of activation thresholds during initial engagement...
  52. ncbi Cloning and functional characterization of the murine caspase-3 gene promoter
    Laurent Sabbagh
    Laboratoire d Immunologie, Centre de Recherche du CHUM, Campus St Luc, Pavillon Edouard Asselin, 264 Boulevard Rene Levesque Est, 1370D, Montreal, Quebec H3C 3J7, Canada
    DNA Cell Biol 25:104-15. 2006
    ..Finally, some of the conserved transcription factor binding sites identified between the human and mouse promoters appear to play an important role in lymphoid cells...
  53. ncbi IL-4 influences the differentiation and the susceptibility to activation-induced cell death of human naive CD8+ T cells
    Catherine Riou
    Laboratoire d Immunologie, Centre de Recherche du CHUM, Pavillon Edouard Asselin, Hopital Saint Luc, 264 René Lévesque Boulevard E, Montreal, Quebec H2X 1P1, Canada
    Int Immunol 18:827-35. 2006
    ....
  54. pmc Poly(I:C) and lipopolysaccharide innate sensing functions of circulating human myeloid dendritic cells are affected in vivo in hepatitis C virus-infected patients
    Ian Gaël Rodrigue-Gervais
    Centre Hospitalier de l Universite de Montreal, Hopital Saint Luc, 264 Boul René Lévesque Est, Rm EA 312, Montreal, Quebec, Canada H2X 1P1
    J Virol 81:5537-46. 2007
    ..Taken together, these data indicate that detection of HCV genomic RNA in DCs and loss of function in the danger signal responsiveness of a small proportion of DCs in vivo are interrelated rather than independent phenomena...
  55. pmc Dissecting the HIV-specific immune response: a systems biology approach
    Yoav Peretz
    ImmuneCarta Services, Montreal, Quebec, Canada
    Curr Opin HIV AIDS 7:17-23. 2012
    ....
  56. pmc Systems analysis of a RIG-I agonist inducing broad spectrum inhibition of virus infectivity
    Marie Line Goulet
    Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Canada
    PLoS Pathog 9:e1003298. 2013
    ....
  57. doi Immunogenicity and tolerability of an inactivated and adjuvanted pandemic H1N1 influenza vaccine, in HIV-1-infected patients
    Cecile L Tremblay
    Centre de recherche du Centre Hospitalier de l Universite de Montreal, Montreal, QC, Canada
    Vaccine 29:1359-63. 2011
    ..There was no correlation between baseline CD4 cell count (p=0.539) or HIV viral load (p=0.381) and immune response. Other vaccine strategies should be evaluated in this HIV population, to improve response rates...
  58. ncbi A defective viral superantigen-presenting phenotype in HLA-DR transfectants is corrected by CIITA
    Georges A Azar
    Laboratoire d Immunologie Moléculaire, Departement de Microbiologie et Immunologie, Faculte de Medecine, Universite de Montreal, Hôpital St Luc, Montreal, Canada
    J Immunol 174:7548-57. 2005
    ..Together, our results suggest that functional vSAgs-DR complexes form after the very late stages of class II maturation, most probably at the cell surface...
  59. pmc Recognition of a defined region within p24 gag by CD8+ T cells during primary human immunodeficiency virus type 1 infection in individuals expressing protective HLA class I alleles
    Hendrik Streeck
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
    J Virol 81:7725-31. 2007
    ....
  60. ncbi Evaluation of OX40 ligand as a costimulator of human antiviral memory CD8 T cell responses: comparison with B7.1 and 4-1BBL
    Lena Serghides
    Department of Immunology, University of Toronto, Toronto, Ontario, Canada
    J Immunol 175:6368-77. 2005
    ..Thus, OX40L costimulates human antiviral memory CD8 T cell responses largely through indirect effects and can enhance anti-influenza, anti-EBV, and anti-HIV responses, particularly in combination with 4-1BBL or B7...
  61. pmc Inhibition of HIV-1 transmission in trans from dendritic cells to CD4+ T lymphocytes by natural antibodies to the CRD domain of DC-SIGN purified from breast milk and intravenous immunoglobulins
    Mary Requena
    Unité INSERM U743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomedicales des Cordeliers, Paris, France
    Immunology 123:508-18. 2008
    ..Our data support a role of natural antibodies to DC-SIGN in the modulation of postnatal HIV transmission through breast-feeding and in the natural host defence against HIV-1 in infected individuals...
  62. ncbi Enhancement of HIV-specific CD8 T cell responses by dual costimulation with CD80 and CD137L
    Jacob Bukczynski
    Department of Immunology, University of Toronto, Toronto, Canada
    J Immunol 175:6378-89. 2005
    ..Taken together, these data suggest that 4-1BBL and B7.1 have additive or synergistic effects on HIV-specific CD8 T cell responses and represent a promising combination for therapeutic vaccination for HIV...
  63. pmc Diminished proliferation of human immunodeficiency virus-specific CD4+ T cells is associated with diminished interleukin-2 (IL-2) production and is recovered by exogenous IL-2
    Christiana Iyasere
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1876, USA
    J Virol 77:10900-9. 2003
    ..These results also suggest that relatively high frequencies of HIV-specific CD4(+) T cells persist in the peripheral blood during viremia, are not replicatively senescent, and proliferate when IL-2 is provided exogenously...

Research Grants6

  1. Novel Concepts for the Eradication of HIV
    RAFICK PIERRE SEKALY; Fiscal Year: 2009
    ..We will identify pathways that can be targeted to purge HIV from its reservoir. This will lead to the development of novel immunological interventions in the treatment of HIV. PUBLIC HEALTH RELEVANCE: Eradication of HI ..
  2. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2006
    ..Overall, we will be able to assess the importance of Ag specific CD4+ memory T cells and of viral escape in the maintenance of persistence CTL response. ..
  3. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2004
    ..Overall, we will be able to assess the importance of Ag specific CD4+ memory T cells and of viral escape in the maintenance of persistence CTL response. ..
  4. Effect of Viral Escape on Memory T Cell Maturation
    RAFICK PIERRE SEKALY; Fiscal Year: 2005
    ..Overall, we will be able to assess the importance of Ag specific CD4+ memory T cells and of viral escape in the maintenance of persistence CTL response. ..