Janet Rossant

Summary

Affiliation: Samuel Lunenfeld Research Institute
Country: Canada

Publications

  1. pmc Lineage allocation and asymmetries in the early mouse embryo
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Philos Trans R Soc Lond B Biol Sci 358:1341-8; discussion 1349. 2003
  2. ncbi request reprint Mouse-based phenogenomics for modelling human disease
    J Rossant
    Dept of Molecular and Medical Genetics, University of Toronto, and Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Ontario M5G 1X5, Toronto, Canada
    Trends Mol Med 7:502-7. 2001
  3. pmc The mouse genome sequence--the end of the tail, or just the beginning?
    Janet Rossant
    Samuel Lunenfeld Research Institute, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Genome Biol 4:109. 2003
  4. ncbi request reprint Placental development: lessons from mouse mutants
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Nat Rev Genet 2:538-48. 2001
  5. ncbi request reprint Mash2 is expressed in oogenesis and preimplantation development but is not required for blastocyst formation
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Mech Dev 73:183-91. 1998
  6. ncbi request reprint Signaling pathways in vascular development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Molecular and Medical Genetics, University of Toronto, 600 University Avenue, Ontario, M5G 1X5, Canada
    Annu Rev Cell Dev Biol 18:541-73. 2002
  7. ncbi request reprint Emerging asymmetry and embryonic patterning in early mouse development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Ontario M5G 1X5, Canada
    Dev Cell 7:155-64. 2004
  8. ncbi request reprint Lineage development and polar asymmetries in the peri-implantation mouse blastocyst
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinal Hospital, Toronto, Ont, Canada
    Semin Cell Dev Biol 15:573-81. 2004
  9. ncbi request reprint Chimeras and mosaics in mouse mutant analysis
    J Rossant
    Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada
    Trends Genet 14:358-63. 1998
  10. ncbi request reprint Vascular development and patterning: making the right choices
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Curr Opin Genet Dev 13:408-12. 2003

Detail Information

Publications160 found, 100 shown here

  1. pmc Lineage allocation and asymmetries in the early mouse embryo
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Philos Trans R Soc Lond B Biol Sci 358:1341-8; discussion 1349. 2003
    ....
  2. ncbi request reprint Mouse-based phenogenomics for modelling human disease
    J Rossant
    Dept of Molecular and Medical Genetics, University of Toronto, and Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Ontario M5G 1X5, Toronto, Canada
    Trends Mol Med 7:502-7. 2001
    ..Integrated, multidisciplinary programs will be needed to fully exploit the power of the mouse in molecular medicine...
  3. pmc The mouse genome sequence--the end of the tail, or just the beginning?
    Janet Rossant
    Samuel Lunenfeld Research Institute, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Genome Biol 4:109. 2003
    ..Each illustrates how the availability of the genome sequence will change the way mouse biologists do business in future...
  4. ncbi request reprint Placental development: lessons from mouse mutants
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Nat Rev Genet 2:538-48. 2001
    ..Furthering our understanding of mouse trophoblast development should provide novel insights into human placental function...
  5. ncbi request reprint Mash2 is expressed in oogenesis and preimplantation development but is not required for blastocyst formation
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Mech Dev 73:183-91. 1998
    ..If Mash2 functions in other aspects of trophoblast development, it must act cooperatively with other factors...
  6. ncbi request reprint Signaling pathways in vascular development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Molecular and Medical Genetics, University of Toronto, 600 University Avenue, Ontario, M5G 1X5, Canada
    Annu Rev Cell Dev Biol 18:541-73. 2002
    ..Further understanding of the complexities of the pathways should help refine such strategies...
  7. ncbi request reprint Emerging asymmetry and embryonic patterning in early mouse development
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Ontario M5G 1X5, Canada
    Dev Cell 7:155-64. 2004
    ..Whether these asymmetries are causally related to embryonic patterning or are coincidental outcomes of the topology of normal development remains uncertain...
  8. ncbi request reprint Lineage development and polar asymmetries in the peri-implantation mouse blastocyst
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinal Hospital, Toronto, Ont, Canada
    Semin Cell Dev Biol 15:573-81. 2004
    ..The exact relationship between lineage segregation, preimplantation polarities and the postimplantation axes remain to be determined but are now open to molecular and cellular investigation...
  9. ncbi request reprint Chimeras and mosaics in mouse mutant analysis
    J Rossant
    Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada
    Trends Genet 14:358-63. 1998
    ....
  10. ncbi request reprint Vascular development and patterning: making the right choices
    Janet Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Curr Opin Genet Dev 13:408-12. 2003
    ....
  11. ncbi request reprint Stem cells from the Mammalian blastocyst
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Stem Cells 19:477-82. 2001
    ..Manipulation of both intrinsic and extrinsic regulators may be needed to reveal the full potential of stem cells from other stages of development and the adult...
  12. pmc Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Dev Cell 22:887-901. 2012
    ..These results provide a comprehensive stem cell proteomic resource and enable new approaches to interrogate the mechanisms that regulate cell fate specification...
  13. ncbi request reprint Porcn-dependent Wnt signaling is not required prior to mouse gastrulation
    Steffen Biechele
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, ON M5G 1X8, Canada
    Development 140:2961-71. 2013
    ....
  14. ncbi request reprint Disruption of early proximodistal patterning and AVE formation in Apc mutants
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Development 133:3379-87. 2006
    ..In addition, we found that nuclear beta-catenin and other molecular markers are asymmetrically expressed by 4.5 days...
  15. ncbi request reprint Live imaging and genetic analysis of mouse notochord formation reveals regional morphogenetic mechanisms
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Dev Cell 13:884-96. 2007
    ..Therefore, we propose three distinct regions within the mouse notochord, each with unique morphogenetic origins...
  16. doi request reprint The Hippo pathway member Nf2 is required for inner cell mass specification
    Katie Cockburn
    Department of Molecular Genetics, University of Toronto, ON M5S 1A8, Canada
    Curr Biol 23:1195-201. 2013
    ..Together, these data establish a clear role for Nf2 upstream of Yap in the preimplantation embryo and demonstrate that Hippo signaling is essential to segregate the ICM from the TE. ..
  17. ncbi request reprint Hedgehog regulates distinct vascular patterning events through VEGF-dependent and -independent mechanisms
    Leigh Coultas
    Developmental and Stem Cell Biology Program, Hospital for Sick Children Research Institute, 555 University Ave, Toronto, Ontario, Canada
    Blood 116:653-60. 2010
    ..These findings establish that Hh uses both VEGF- and Notch-dependent and -independent mechanisms to pattern specific events in early blood vascular development...
  18. ncbi request reprint Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst
    Dan Strumpf
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Ontario, Canada
    Development 132:2093-102. 2005
    ..Thus, Cdx2 is essential for segregation of the ICM and TE lineages at the blastocyst stage by ensuring repression of Oct4 and Nanog in the TE...
  19. pmc Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry
    Jun K Takeuchi
    Department of Cardiovascular Research, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    Proc Natl Acad Sci U S A 104:846-51. 2007
    ..Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry...
  20. ncbi request reprint Vascular endothelial growth factor directly inhibits primitive neural stem cell survival but promotes definitive neural stem cell survival
    Tamaki Wada
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 3E1
    J Neurosci 26:6803-12. 2006
    ..In conclusion, VEGF-A function through Flk1 mediates survival (and not proliferative or fate change) effects on NSCs, specifically...
  21. ncbi request reprint Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, Toronto, ON M5G 1L7, Canada
    Development 137:395-403. 2010
    ....
  22. ncbi request reprint Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, 101 College St, Toronto, ON, Canada
    Dev Biol 313:614-29. 2008
    ..Cdx2 therefore appears to act downstream of the first lineage decision, suggesting that processes influencing lineage allocation or morphogenesis may regulate Cdx2 expression along the inside/outside axis of the embryo...
  23. pmc Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes
    Brian Cox
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada
    Mol Syst Biol 3:109. 2007
    ..These targets are evidence that Nmyc is a regulator of genes involved in mRNA processing and a repressor of the imprinted gene Igf2r in the developing lung...
  24. ncbi request reprint Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers...
  25. pmc Enu mutagenesis identifies a novel platelet phenotype in a loss-of-function Jak2 allele
    Nicole M Anderson
    Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 8:e75472. 2013
    ..Our data suggest that perturbing JAK2 activation may have unexpected consequences in elevation of platelet number and correspondingly, important implications for treatment of hematological disorders with constitutive Jak2 activity...
  26. ncbi request reprint Dissecting Wnt/beta-catenin signaling during gastrulation using RNA interference in mouse embryos
    Heiko Lickert
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M5G 1X5, Canada
    Development 132:2599-609. 2005
    ..This functional genomic approach allows the rapid identification of functionally important components of embryonic development from large datasets of putative targets...
  27. doi request reprint Porcupine homolog is required for canonical Wnt signaling and gastrulation in mouse embryos
    Steffen Biechele
    Department of Molecular Genetics, University of Toronto, ON, Canada
    Dev Biol 355:275-85. 2011
    ..Taken together, these data confirm the importance of Porcn for Wnt secretion and gastrulation and suggest that disruption of early development underlies the male lethality of human PORCN mutants...
  28. ncbi request reprint Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis development
    Pantelis Georgiades
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Development 133:1059-68. 2006
    ..This study provides new insights about the genetic and cellular basis of the patterning role and development of the early trophoblast...
  29. ncbi request reprint Foxh1 is essential for development of the anterior heart field
    Ingo Von Both
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Dev Cell 7:331-45. 2004
    ..Thus, Foxh1 and Nkx2-5 functionally interact and are essential for development of the AHF and its derivatives, the RV and OFT, in response to TGFbeta-like signals...
  30. pmc Translational analysis of mouse and human placental protein and mRNA reveals distinct molecular pathologies in human preeclampsia
    Brian Cox
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
    Mol Cell Proteomics 10:M111.012526. 2011
    ..We conclude that clinically similar preeclampsia patients exhibit divergent placental gene expression profiles thus implicating divergent molecular mechanisms in the origins of this disease...
  31. ncbi request reprint Baf60c is essential for function of BAF chromatin remodelling complexes in heart development
    Heiko Lickert
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nature 432:107-12. 2004
    ....
  32. ncbi request reprint Vascular endothelial growth factor a signaling in the podocyte-endothelial compartment is required for mesangial cell migration and survival
    Vera Eremina
    Department of Maternal and Fetal Health, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Room 855Q, 600 University of Toronto, Ontario, Canada M5G 1X5
    J Am Soc Nephrol 17:724-35. 2006
    ..Together, these results demonstrate a key role for VEGF-A production in the podocyte for mesangial cell survival and differentiation...
  33. doi request reprint Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin
    Robert Odell Stephenson
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Development 137:3383-91. 2010
    ..They also show that Cdx2 expression is strongly linked to apical membrane polarization...
  34. pmc Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis
    Keli Xu
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
    Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation...
  35. ncbi request reprint Activated Fps/Fes partially rescues the in vivo developmental potential of Flk1-deficient vascular progenitor cells
    Jody J Haigh
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Ave, Toronto, Ontario, Canada M5G 1X5
    Blood 103:912-20. 2004
    ..We have also demonstrated that activated Fps/Fes causes hemangioma formation in vivo, independently of Flk1, as a result of increasing vascular progenitor density...
  36. pmc Comparative systems biology of human and mouse as a tool to guide the modeling of human placental pathology
    Brian Cox
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, Ontario, Canada
    Mol Syst Biol 5:279. 2009
    ..The entire data are available as a web-accessible database to guide the informed development of mouse models to study human disease...
  37. ncbi request reprint Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors
    Masatsugu Ema
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Blood 107:111-7. 2006
    ..Thus, Flk1 expression marks progenitors with broad mesodermal potential but is not absolutely required for the development of all mesodermal lineages in which it is expressed...
  38. doi request reprint Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
    Nat Methods 6:370-6. 2009
    ..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2...
  39. pmc Cdx2 is essential for axial elongation in mouse development
    Kallayanee Chawengsaksophak
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Proc Natl Acad Sci U S A 101:7641-5. 2004
    ..The gene appears to be important in the integration of the pathways controlling embryonic axial elongation, and anterior-posterior patterning...
  40. pmc Zfp206 regulates ES cell gene expression and differentiation
    Wen Zhang
    Department of Molecular and Medical Genetics, University of Toronto, 4388 Medical Sciences Building, 1 King s College Circle, Toronto, ON M5S 1A8, Canada
    Nucleic Acids Res 34:4780-90. 2006
    ..Together, these data indicate that Zfp206 is a regulator of ES cell differentiation that controls a set of genes expressed very early in development, most of which themselves appear to be regulators...
  41. ncbi request reprint Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling
    Thomas Kislinger
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada
    Cell 125:173-86. 2006
    ..This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal...
  42. doi request reprint A novel Phex mutation in a new mouse model of hypophosphatemic rickets
    Celeste Owen
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Cell Biochem 113:2432-41. 2012
    ....
  43. pmc Making the blastocyst: lessons from the mouse
    Katie Cockburn
    Department of Molecular Genetics, University of Toronto, Canada
    J Clin Invest 120:995-1003. 2010
    ..A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells...
  44. ncbi request reprint Genome-wide analysis of mouse transcripts using exon microarrays and factor graphs
    Brendan J Frey
    Electrical and Computer Engineering, University of Toronto, 10 King s College Rd, Toronto, Ontario M5S 3G4, Canada
    Nat Genet 37:991-6. 2005
    ..GenRate also detected tens of thousands of potential new exons and reconciled discrepancies in current cDNA databases by 'stitching' new transcribed regions into previously annotated genes...
  45. pmc Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryo
    Kashif Ahmed
    Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada
    PLoS ONE 5:e10531. 2010
    ..Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells...
  46. doi request reprint Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
    Janet Rossant
    Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
    Development 136:701-13. 2009
    ..We also discuss the continuing debate that surrounds the relative impacts of early lineage bias versus the stochastic allocation of cells with respect to the events that pattern the blastocyst and initiate its later asymmetries...
  47. doi request reprint FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Development 137:715-24. 2010
    ..In conclusion, we propose a model in which stochastic and progressive specification of EPI and PE lineages occurs during maturation of the blastocyst in an FGF/MAP kinase signal-dependent manner...
  48. pmc Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo
    Peter J Rugg-Gunn
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, ON, Canada
    Proc Natl Acad Sci U S A 107:10783-90. 2010
    ..These studies indicate that histone modification mechanisms may differ between early embryo lineages and emphasize the importance of examining in vivo and in vitro progenitor cells...
  49. pmc The tumor suppressor gene Smad4/Dpc4 is required for gastrulation and later for anterior development of the mouse embryo
    C Sirard
    Amgen Institute, Toronto, Ontario M5G 2C1, Canada
    Genes Dev 12:107-19. 1998
    ..Rescued embryos show severe anterior truncations, indicating a second important role for Smad4 in anterior patterning during embryogenesis...
  50. ncbi request reprint Location of transient ectodermal progenitor potential in mouse development
    Lingyu Li
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Development 140:4533-43. 2013
    ..Our work not only improves our understanding of ectodermal layer development in early embryos, but also provides a framework for regenerative differentiation towards ectodermal tissues. ..
  51. doi request reprint The role of FGF/Erk signaling in pluripotent cells
    Fredrik Lanner
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada
    Development 137:3351-60. 2010
    ..Understanding how extrinsic signals such as FGF regulate different stem cell states will be crucial to harvest the clinical promise of induced pluripotent and embryo-derived stem cells...
  52. ncbi request reprint Mash2 acts cell autonomously in mouse spongiotrophoblast development
    M Tanaka
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Dev Biol 190:55-65. 1997
    ....
  53. doi request reprint Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord
    Owen J Tamplin
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    Dev Biol 360:415-25. 2011
    ..The combination of highly specific gene expression profiling and genome-wide ChIP analysis is a powerful means of understanding developmental pathways, even for small cell populations such as the notochord...
  54. ncbi request reprint Adhesion is prerequisite, but alone insufficient, to elicit stem cell pluripotency
    Phillip Karpowicz
    Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Neurosci 27:5437-47. 2007
    ..These adhesive differences suggest an evolving compartmentalization in multipotent NSCs during development and serve to illustrate the importance of cell-cell association for revealing cellular contribution...
  55. pmc Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives
    Owen J Tamplin
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    BMC Genomics 9:511. 2008
    ..However, the targets of Foxa2 during embryogenesis, and the molecular impact of organizer loss on the gastrula embryo, have not been well defined...
  56. pmc Trophoblast expression of fms-like tyrosine kinase 1 is not required for the establishment of the maternal-fetal interface in the mouse placenta
    Masanori Hirashima
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, ON, Canada M5G 1X5
    Proc Natl Acad Sci U S A 100:15637-42. 2003
    ..Targeting maternal sFlt1 levels for treatment of preeclampsia may thus be possible without affecting the proper formation of the placenta...
  57. pmc The Centre for Modeling Human Disease Gene Trap resource
    Christine To
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 32:D557-9. 2004
    ..asp). The clones are available as a resource to researchers worldwide to help to functionally annotate the mammalian genome and will serve as a source to test candidate loci identified by phenotype-driven mutagenesis screens...
  58. ncbi request reprint Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells
    Cheryle A Séguin
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1L7, Canada
    Cell Stem Cell 3:182-95. 2008
    ..These endoderm progenitor cells maintain a stable phenotype through many passages in culture, thereby providing new tools to explore the pathways of endoderm differentiation...
  59. ncbi request reprint Characterization of a gene trap insertion into a novel gene, cordon-bleu, expressed in axial structures of the gastrulating mouse embryo
    S Gasca
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Dev Genet 17:141-54. 1995
    ..Therefore, the product of the cobl gene may also prove to be an important component of the genetic pathway regulating vertebrate axis formation...
  60. ncbi request reprint Genomic imprinting of Mash2, a mouse gene required for trophoblast development
    F Guillemot
    Samuel Lunenfield Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Genet 9:235-42. 1995
    ..5 days post-coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin-2, insulin-like growth factor-2 and H19...
  61. ncbi request reprint The tumor suppressor gene Brca1 is required for embryonic cellular proliferation in the mouse
    R Hakem
    Amgen Institute, Toronto, Ontario, Canada
    Cell 85:1009-23. 1996
    ..Thus, the death of Brca1(5-6) mutant embryos prior to gastrulation may be due to a failure of the proliferative burst required for the development of the different germ layers...
  62. ncbi request reprint Notch1 is required for the coordinate segmentation of somites
    R A Conlon
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Development 121:1533-45. 1995
    ..However, we have found that somitogenesis is delayed and disorganized in Notch1 mutant embryos. We propose that Notch1 normally coordinates the process of somitogenesis, and we provide a model of how this might occur...
  63. ncbi request reprint Interaction of the TEK and TIE receptor tyrosine kinases during cardiovascular development
    M C Puri
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Ontario, Canada M5G 1X5
    Development 126:4569-80. 1999
    ..This analysis demonstrates essential functions for TEK and TIE in maintaining the integrity of the mature vasculature...
  64. pmc Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein
    Amy P Wong
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Nat Biotechnol 30:876-82. 2012
    ..Our study provides a method for generating patient-specific airway epithelial cells for disease modeling and in vitro drug testing...
  65. ncbi request reprint Stem cells and lineage development in the mammalian blastocyst
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Reprod Fertil Dev 19:111-8. 2007
    ....
  66. ncbi request reprint Gene trapping in embryonic stem cells
    William L Stanford
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Methods Enzymol 420:136-62. 2006
    ....
  67. ncbi request reprint Cell and molecular regulation of the mouse blastocyst
    Yojiro Yamanaka
    Program of Developmental Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Dev Dyn 235:2301-14. 2006
    ..Together, these mechanisms guide the differentiation of the first lineages of the mouse and thereby set up tissues that will be important for the first steps of embryonic body patterning...
  68. ncbi request reprint Cell fate decisions in early blood vessel formation
    Masatsugu Ema
    Samuel Lunenfeld Research Institute and the Department of Molecular and Medical Genetics, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Trends Cardiovasc Med 13:254-9. 2003
    ....
  69. ncbi request reprint Gene-trap expression screening to identify endothelial-specific genes
    Masanori Hirashima
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Blood 104:711-8. 2004
    ..Gene-trap expression screening can thus be used to identify early endothelial-specific genes and analyze their function in mice...
  70. ncbi request reprint How signaling promotes stem cell survival: trophoblast stem cells and Shp2
    Amy Ralston
    The Hospital for Sick Children, and Department of Medical Genetics and Microbiology, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    Dev Cell 10:275-6. 2006
    ..These studies provide a novel link between FGF/Erk signaling and cell survival that may be relevant to other stem and progenitor cell niches...
  71. pmc High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivo in mice
    John C Slevin
    Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Canada
    BMC Dev Biol 6:10. 2006
    ..This method may be useful for determining the developmental effects of targeted genetic or cellular interventions at critical stages of placentation, gastrulation, axis formation, and neural tube closure...
  72. ncbi request reprint Endothelial cells and VEGF in vascular development
    Leigh Coultas
    The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8 Canada
    Nature 438:937-45. 2005
    ....
  73. pmc Three-dimensional analysis of vascular development in the mouse embryo
    Johnathon R Walls
    Mouse Imaging Centre, Hospital for Sick Children, Toronto Centre for Phenogenomics, Toronto, Ontario, Canada
    PLoS ONE 3:e2853. 2008
    ..0 and E10.0. This atlas is freely available at http://www.mouseimaging.ca/research/mouse_atlas.html and will serve as a platform to provide insight into normal and abnormal vascular development...
  74. ncbi request reprint Mouse trophoblast stem cells
    Jennifer Quinn
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Ontario, Canada
    Methods Mol Med 121:125-48. 2006
    ..This chapter will discuss the initial derivation of trophoblast stem cells from the blastocyst stage, maintenance, differentiation, flow cytometry and transfection techniques that can be used with these cells...
  75. pmc Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos
    Marina Gertsenstein
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 5:e11260. 2010
    ..Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources...
  76. doi request reprint The V-ATPase a3 subunit mutation R740S is dominant negative and results in osteopetrosis in mice
    Noelle Ochotny
    Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
    J Bone Miner Res 26:1484-93. 2011
    ..These results suggest that the V(0) complex has proton-pumping-independent functions in mammalian cells...
  77. ncbi request reprint Essential role of Mash-2 in extraembryonic development
    F Guillemot
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 371:333-6. 1994
    ..Mash-2 is the first transcription factor shown to play a critical part in the development of the mammalian trophoblast lineage...
  78. ncbi request reprint Otx2 and HNF3beta genetically interact in anterior patterning
    O Jin
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Int J Dev Biol 45:357-65. 2001
    ..In addition, the forebrain truncations suggest an involvement of both genes in anterior patterning, through their overlapping expression domains in either the AVE and/or the prechordal mesoderm...
  79. ncbi request reprint A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis
    F Shalaby
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cell 89:981-90. 1997
    ....
  80. ncbi request reprint Parental origin-specific expression of Mash2 is established at the time of implantation with its imprinting mechanism highly resistant to genome-wide demethylation
    M Tanaka
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Mech Dev 87:129-42. 1999
    ..Our results suggest the possibility that a mechanism other than DNA methylation, such as allele-specific chromatin conformation, may be involved in maintenance of parental origin-specific expression of Mash2...
  81. pmc The receptor tyrosine kinase TIE is required for integrity and survival of vascular endothelial cells
    M C Puri
    Program of Molecular Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    EMBO J 14:5884-91. 1995
    ..TIE is not essential, however, for vasculogenesis, the early differentiation of endothelial cells...
  82. ncbi request reprint HNF-3 beta is essential for node and notochord formation in mouse development
    S L Ang
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cell 78:561-74. 1994
    ..HNF-3 beta is not required for the development of definitive endoderm cells, but foregut morphogenesis is severely affected in HNF-3 beta -/- embryos...
  83. ncbi request reprint Two mouse mutations mapped to chromosome 11 with differing morphologies but similar progressive inflammatory alopecia
    Geoffrey A Wood
    Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Exp Dermatol 14:373-9. 2005
    ..The early onset of the disease, predictable progression, and differences in inflammatory cell localization between the two mutants make these mice particularly useful models for inflammatory hair loss and autoimmune diseases in general...
  84. ncbi request reprint Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts
    Tilo Kunath
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada
    Development 132:1649-61. 2005
    ..Based on gene expression analysis, chimera studies and imprinted X-inactivation, XEN cell lines are representative of extra-embryonic endoderm and provide a new cell culture model of an early mammalian lineage...
  85. ncbi request reprint Defects in heart and lung development in compound heterozygotes for two different targeted mutations at the N-myc locus
    C B Moens
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada
    Development 119:485-99. 1993
    ..This study illustrates the importance of generating different mutations at a given locus to elucidate fully the function of a particular gene during development...
  86. ncbi request reprint fgfr-1 is required for embryonic growth and mesodermal patterning during mouse gastrulation
    T P Yamaguchi
    Samuel Lunenfeld Research Institute SLRI, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 8:3032-44. 1994
    ..These results suggest that FGFR-1 transduces signals that specify mesodermal cell fates and regional patterning of the mesoderm during gastrulation...
  87. ncbi request reprint The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis
    D Dufort
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5
    Development 125:3015-25. 1998
    ..We show that such mutant embryos lack foregut and midgut endoderm. In addition, left-right asymmetry is affected in the mutant embryos...
  88. ncbi request reprint A targeted mutation reveals a role for N-myc in branching morphogenesis in the embryonic mouse lung
    C B Moens
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 6:691-704. 1992
    ....
  89. ncbi request reprint Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype
    Tilo Kunath
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5
    Nat Biotechnol 21:559-61. 2003
    ..Such embryos, in which endogenous p120-Ras GTPase-activating protein (RasGAP), encoded by Rasa1 (also known as RasGAP), was silenced, had the same phenotype as did the previously reported Rasa1 null mutation...
  90. pmc Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse
    Masatsugu Ema
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada M5G 1X5
    Genes Dev 17:380-93. 2003
    ..We propose a model in which the combinatorial effects of Flk1 and Tal1 act to regulate cell fate choice in early development into hematopoietic, endothelial, and smooth muscle lineages...
  91. ncbi request reprint Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Curr Biol 8:661-4. 1998
    ..This, and the possibility of subsequent lineage-specific or conditional allele repair in situ, represent new genome modification strategies that can be used to investigate multiple functions of a single gene...
  92. ncbi request reprint Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice
    Q Ding
    Program in Developmental Biology and Division of Endocrinology, Research Institute, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Development 125:2533-43. 1998
    ..Our results imply that Gli2 is required to mediate high level but not low level Shh activity and show that the development of motor neurons can occur in the absence of floor plate induction...
  93. pmc Mining mouse microarray data
    D A Wigle
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, MsS 1A8, Canada
    Genome Biol 2:REVIEWS1019. 2001
    ..Microarray data posted on the internet can be reanalyzed to study a range of questions...
  94. ncbi request reprint FGF signaling in mouse gastrulation and anteroposterior patterning
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cold Spring Harb Symp Quant Biol 62:127-33. 1997
  95. ncbi request reprint Conservation of the Notch signalling pathway in mammalian neurogenesis
    J L de la Pompa
    Amgen Institute, Toronto, Ontario, Canada
    Development 124:1139-48. 1997
    ..Our results demonstrate conservation of the Notch pathway and its regulatory mechanisms from fly to mouse, and support a role for the murine Notch signalling pathway in the regulation of neural stem cell differentiation...
  96. ncbi request reprint Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium
    G H Fong
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 376:66-70. 1995
    ..We suggest that the Flt-1 signalling pathway may regulate normal endothelial cell-cell or cell-matrix interactions during vascular development...
  97. ncbi request reprint Chimaeras and mosaics for dissecting complex mutant phenotypes
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Int J Dev Biol 45:577-82. 2001
    ..Our views on chimaeras presented below definitely contain elements which grew out from this principle...
  98. ncbi request reprint The basic-helix-loop-helix protein pod1 is critically important for kidney and lung organogenesis
    S E Quaggin
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5
    Development 126:5771-83. 1999
    ..Defects specific to later specialized cell types where Pod1 is expressed, such as the podocytes, were also observed, suggesting that this transcription factor may play multiple roles in kidney morphogenesis...
  99. ncbi request reprint Stem cells and early lineage development
    Janet Rossant
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Department of Molecular Genetics, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
    Cell 132:527-31. 2008
    ..Understanding the initial developmental status of the different pluripotent lines is critical for defining starting conditions for differentiation toward therapeutically relevant cell types...
  100. ncbi request reprint Anterior mesendoderm induces mouse Engrailed genes in explant cultures
    S L Ang
    Division of Molecular and Medical Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Development 118:139-49. 1993
    ..These results provide the first direct evidence for a role of mesendoderm in induction and regionalization of neural tissue in mouse...
  101. ncbi request reprint Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice
    F Shalaby
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 376:62-6. 1995
    ..These results indicate that Flk-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo...