Andras Nagy

Summary

Affiliation: Samuel Lunenfeld Research Institute
Country: Canada

Publications

  1. doi request reprint c-Myb-dependent smooth muscle cell differentiation
    Karolina M Kolodziejska
    Heart and Stroke Richard Lewar Center of Excellence in Cardiovascular Research, Department of Medicine, University of Toronto, Canada
    Circ Res 102:554-61. 2008
  2. pmc Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos
    Marina Gertsenstein
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 5:e11260. 2010
  3. pmc Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
    Gusztav Belteki
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Nucleic Acids Res 33:e51. 2005
  4. ncbi request reprint Chimaeras and mosaics for dissecting complex mutant phenotypes
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Int J Dev Biol 45:577-82. 2001
  5. ncbi request reprint Tailoring the genome: the power of genetic approaches
    Andras Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Genet 33:276-84. 2003
  6. ncbi request reprint Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Curr Biol 8:661-4. 1998
  7. pmc Highly efficient site-specific transgenesis in cancer cell lines
    Iacovos P Michael
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
    Mol Cancer 11:89. 2012
  8. ncbi request reprint Vascular endothelial growth factor a signaling in the podocyte-endothelial compartment is required for mesangial cell migration and survival
    Vera Eremina
    Department of Maternal and Fetal Health, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Room 855Q, 600 University of Toronto, Ontario, Canada M5G 1X5
    J Am Soc Nephrol 17:724-35. 2006
  9. ncbi request reprint Hedgehog regulates distinct vascular patterning events through VEGF-dependent and -independent mechanisms
    Leigh Coultas
    Developmental and Stem Cell Biology Program, Hospital for Sick Children Research Institute, 555 University Ave, Toronto, Ontario, Canada
    Blood 116:653-60. 2010
  10. ncbi request reprint Vascular endothelial growth factor directly inhibits primitive neural stem cell survival but promotes definitive neural stem cell survival
    Tamaki Wada
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 3E1
    J Neurosci 26:6803-12. 2006

Collaborators

Detail Information

Publications107 found, 100 shown here

  1. doi request reprint c-Myb-dependent smooth muscle cell differentiation
    Karolina M Kolodziejska
    Heart and Stroke Richard Lewar Center of Excellence in Cardiovascular Research, Department of Medicine, University of Toronto, Canada
    Circ Res 102:554-61. 2008
    ..Our data are the first to implicate c-Myb in SMC differentiation from precursor stem cell-derived populations, reinforcing its potential role in phenotypic modulation of SMCs and vascular disease...
  2. pmc Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos
    Marina Gertsenstein
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 5:e11260. 2010
    ..Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources...
  3. pmc Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
    Gusztav Belteki
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Nucleic Acids Res 33:e51. 2005
    ..The presented approach abrogates the need to generate tissue-specific rtTA transgenes for tissues where well-characterized Cre lines already exist...
  4. ncbi request reprint Chimaeras and mosaics for dissecting complex mutant phenotypes
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Int J Dev Biol 45:577-82. 2001
    ..Our views on chimaeras presented below definitely contain elements which grew out from this principle...
  5. ncbi request reprint Tailoring the genome: the power of genetic approaches
    Andras Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Genet 33:276-84. 2003
    ..The genomes of each of these four models have been sequenced, and all have well developed methods of efficient genetic manipulations...
  6. ncbi request reprint Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles
    A Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Curr Biol 8:661-4. 1998
    ..This, and the possibility of subsequent lineage-specific or conditional allele repair in situ, represent new genome modification strategies that can be used to investigate multiple functions of a single gene...
  7. pmc Highly efficient site-specific transgenesis in cancer cell lines
    Iacovos P Michael
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
    Mol Cancer 11:89. 2012
    ....
  8. ncbi request reprint Vascular endothelial growth factor a signaling in the podocyte-endothelial compartment is required for mesangial cell migration and survival
    Vera Eremina
    Department of Maternal and Fetal Health, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Room 855Q, 600 University of Toronto, Ontario, Canada M5G 1X5
    J Am Soc Nephrol 17:724-35. 2006
    ..Together, these results demonstrate a key role for VEGF-A production in the podocyte for mesangial cell survival and differentiation...
  9. ncbi request reprint Hedgehog regulates distinct vascular patterning events through VEGF-dependent and -independent mechanisms
    Leigh Coultas
    Developmental and Stem Cell Biology Program, Hospital for Sick Children Research Institute, 555 University Ave, Toronto, Ontario, Canada
    Blood 116:653-60. 2010
    ..These findings establish that Hh uses both VEGF- and Notch-dependent and -independent mechanisms to pattern specific events in early blood vascular development...
  10. ncbi request reprint Vascular endothelial growth factor directly inhibits primitive neural stem cell survival but promotes definitive neural stem cell survival
    Tamaki Wada
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 3E1
    J Neurosci 26:6803-12. 2006
    ..In conclusion, VEGF-A function through Flk1 mediates survival (and not proliferative or fate change) effects on NSCs, specifically...
  11. ncbi request reprint Cre recombinase specificity defined by the tau locus
    Ella Korets-Smith
    Samuel Lunenfeld Research Institute, 600 University Ave, Toronto, Ontario, M5G 1X5, Canada
    Genesis 40:131-8. 2004
    ..In spite of the observed variability, our data suggest that the tau::Cre mouse line can be used for pan-neuronal recombination of floxed alleles when it is used with caution...
  12. ncbi request reprint Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals
    Kristina Vintersten
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genesis 40:241-6. 2004
    ....
  13. doi request reprint Soluble Flt-1 regulates Flk-1 activation to control hematopoietic and endothelial development in an oxygen-responsive manner
    Kelly A Purpura
    Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Stem Cells 26:2832-42. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  14. pmc Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis
    Keli Xu
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
    Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation...
  15. pmc Developmental and adult phenotyping directly from mutant embryonic stem cells
    Sophia H L George
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Proc Natl Acad Sci U S A 104:4455-60. 2007
    ....
  16. ncbi request reprint Activated Fps/Fes partially rescues the in vivo developmental potential of Flk1-deficient vascular progenitor cells
    Jody J Haigh
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Ave, Toronto, Ontario, Canada M5G 1X5
    Blood 103:912-20. 2004
    ..We have also demonstrated that activated Fps/Fes causes hemangioma formation in vivo, independently of Flk1, as a result of increasing vascular progenitor density...
  17. pmc MBNL proteins repress ES-cell-specific alternative splicing and reprogramming
    Hong Han
    Banting and Best Department of Medical Research and Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Nature 498:241-5. 2013
    ....
  18. ncbi request reprint Cortical and retinal defects caused by dosage-dependent reductions in VEGF-A paracrine signaling
    Jody J Haigh
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Canada
    Dev Biol 262:225-41. 2003
    ..VEGF-A dosage is therefore a critical parameter regulating the density of the vascular plexus in the developing CNS that is in turn a key determinant in the development and architectural organization of the nervous system...
  19. ncbi request reprint A specific requirement for PDGF-C in palate formation and PDGFR-alpha signaling
    Hao Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Genet 36:1111-6. 2004
    ..Our results also show that PDGF-C signaling is a new pathway in palatogenesis, different from, and independent of, those previously implicated...
  20. doi request reprint Adipose vascular endothelial growth factor regulates metabolic homeostasis through angiogenesis
    Hoon Ki Sung
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON M5T 3H7, Canada
    Cell Metab 17:61-72. 2013
    ..Besides revealing insights into the molecular mechanisms of obesity-related metabolic diseases, this study points to the therapeutic potential of increased adipose angiogenesis...
  21. doi request reprint PhiC31 integrase facilitates genetic approaches combining multiple recombinases
    Claudio Monetti
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Methods 53:380-5. 2011
    ..This article describes how to create "dockable" mouse embryonic stem (ES) cell lines, integrate incoming vectors, and analyze the resulting clones. Current applications of this technology are also discussed...
  22. pmc Enhanced natural-killer cell and erythropoietic activities in VEGF-A-overexpressing mice delay F-MuLV-induced erythroleukemia
    David Cervi
    Department of Molecular and Cellular Biology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada
    Blood 109:2139-46. 2007
    ..We propose that a compensatory erythropoietic response combined with increased natural killer (NK) cell activity account for the extended survival of erythroleukemic, Vegfhi/+ mice...
  23. pmc Oct4 is required ~E7.5 for proliferation in the primitive streak
    Brian DeVeale
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    PLoS Genet 9:e1003957. 2013
    ..Impaired expansion of the primitive streak occurs coincident with Oct4 depletion ∼E7.5 and precedes deficient convergent extension which contributes to several aspects of the phenotype...
  24. ncbi request reprint Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein
    Hao Ding
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, M5G 1X5, Canada
    Cell 117:873-86. 2004
    ..spretus chromosomes in F1 cells. We conclude that Rtel is an essential gene that regulates telomere length and prevents genetic instability...
  25. pmc Induced pluripotent stem cell lines derived from equine fibroblasts
    Kristina Nagy
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Stem Cell Rev 7:693-702. 2011
    ..EiPS cell could also potentially be used to revive recently extinct or currently threatened equine species...
  26. doi request reprint Functional genomics reveals a BMP-driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming
    Payman Samavarchi-Tehrani
    Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Cell Stem Cell 7:64-77. 2010
    ..These studies thus define a multistep mechanism that incorporates a BMP-miRNA-MET axis during somatic cell reprogramming. PAPERCLIP:..
  27. ncbi request reprint Nestin is not essential for development of the CNS but required for dispersion of acetylcholine receptor clusters at the area of neuromuscular junctions
    Paria Mohseni
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5T 3H7 Canada
    J Neurosci 31:11547-52. 2011
    ....
  28. ncbi request reprint Hypoxia and hypoxia-inducible factor-1 target genes in central nervous system radiation injury: a role for vascular endothelial growth factor
    Robert A Nordal
    Department of Radiation Oncology, Sunnybrook and Women s College Health Sciences Center, University of Toronto, Toronto, Ontario, Canada
    Clin Cancer Res 10:3342-53. 2004
    ..Because hypoxia mediates VEGF up-regulation through hypoxia-inducible factor-1alpha (HIF1alpha) induction, we studied the relationships of hypoxia, HIF1alpha expression, and expression of VEGF in this damage pathway...
  29. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  30. ncbi request reprint Passaged human chondrocytes accumulate extracellular matrix when induced by bovine chondrocytes
    Nazish Ahmed
    CIHR BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada
    J Tissue Eng Regen Med 4:233-41. 2010
    ..These data suggest that it may be possible to use autologous chondrocytes obtained from osteoarthritic cartilage to form tissue suitable to use for cartilage repair...
  31. doi request reprint Vascular endothelial growth factor--a positive and negative regulator of tumor growth
    Laura M Vecchiarelli-Federico
    Department of Medical Biophysics, Division of Molecular and Cellular Biology, University of Toronto, Sunnybrook Health Sciences Centre, Toronto, Ontario M4N 3M5, Canada
    Cancer Res 70:863-7. 2010
    ..In light of these recent findings, the various mechanisms underlying the negative role of VEGF during early tumor development, progression, and metastasis will be discussed...
  32. pmc Aggregation chimeras: combining ES cells, diploid, and tetraploid embryos
    Mika Tanaka
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada
    Methods Mol Biol 530:287-309. 2009
    ..Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved...
  33. doi request reprint Production of mouse chimeras by aggregating pluripotent stem cells with embryos
    Andras Nagy
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada
    Methods Enzymol 476:123-49. 2010
    ..Since most of the large knockout projects are using the C57BL/6 background, we will pay special attention to cell lines derived from this inbred strain...
  34. ncbi request reprint Genomic imprinting of Mash2, a mouse gene required for trophoblast development
    F Guillemot
    Samuel Lunenfield Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Genet 9:235-42. 1995
    ..5 days post-coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin-2, insulin-like growth factor-2 and H19...
  35. pmc An induction gene trap screen in embryonic stem cells: Identification of genes that respond to retinoic acid in vitro
    L M Forrester
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Proc Natl Acad Sci U S A 93:1677-82. 1996
    ..These studies demonstrate the potential of this induction gene trap approach for identifying and mutating genes downstream of signal transduction pathways...
  36. ncbi request reprint Murine embryonic stem cells
    Andras Nagy
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada
    Methods Enzymol 418:3-21. 2006
    ....
  37. doi request reprint Human embryonic fibroblasts support single cell enzymatic expansion of human embryonic stem cells in xeno-free cultures
    Mark Kibschull
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, M5T 3H7, Canada
    Stem Cell Res 6:70-82. 2011
    ..Our study clearly shows that the source of human fibroblasts is essential for long-term xeno-free hESC maintenance...
  38. doi request reprint Microenvironment-mediated reversion of epiblast stem cells by reactivation of repressed JAK-STAT signaling
    Kento Onishi
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada
    Integr Biol (Camb) 4:1367-76. 2012
    ..Our findings demonstrate that signaling pathway activation and repression create barriers to cell fate transitions that can be overcome by microenvironmental control...
  39. doi request reprint Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures
    David A Fluri
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    Nat Methods 9:509-16. 2012
    ..This method is an important step toward the development of robust PSC generation, expansion and differentiation technology...
  40. pmc Bone marrow transplantation results in human donor blood cells acquiring and displaying mouse recipient class I MHC and CD45 antigens on their surface
    Nobuko Yamanaka
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 4:e8489. 2009
    ..These events have not been investigated in hematopoietic stem cell transplant models...
  41. ncbi request reprint Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells
    Cheryle A Séguin
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1L7, Canada
    Cell Stem Cell 3:182-95. 2008
    ..These endoderm progenitor cells maintain a stable phenotype through many passages in culture, thereby providing new tools to explore the pathways of endoderm differentiation...
  42. ncbi request reprint Site-specific cassette exchange and germline transmission with mouse ES cells expressing phiC31 integrase
    Gusztav Belteki
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Biotechnol 21:321-4. 2003
    ..The product sites of the recombination event (attL and attR) are not substrates for the integrase. Therefore, the phiC31 integrase is ideal to facilitate site-specific insertions into the mammalian genome...
  43. pmc Glomerular-specific alterations of VEGF-A expression lead to distinct congenital and acquired renal diseases
    Vera Eremina
    The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Clin Invest 111:707-16. 2003
    ..Our data demonstrate that tight regulation of VEGF-A signaling is critical for establishment and maintenance of the glomerular filtration barrier and strongly supports a pivotal role for VEGF-A in renal disease...
  44. pmc Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal
    Anna Katerina Hadjantonakis
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    BMC Biotechnol 2:11. 2002
    ..We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice 2...
  45. pmc The generation of definitive neural stem cells from PiggyBac transposon-induced pluripotent stem cells can be enhanced by induction of the NOTCH signaling pathway
    Ryan P Salewski
    Division of Genetics and Development, Toronto Western Research Institute, Toronto, Canada
    Stem Cells Dev 22:383-96. 2013
    ..This study suggests the intrinsic differences between ESCs and iPSCs in their ability to acquire a dNSC fate that can be overcome by inducing the NOTCH pathway...
  46. doi request reprint Elevated coding mutation rate during the reprogramming of human somatic cells into induced pluripotent stem cells
    Junfeng Ji
    Cancer Genomics, Ontario Institute for Cancer Research, Toronto, Ontario, Canada
    Stem Cells 30:435-40. 2012
    ..Thus the factor induced reprogramming stress contributes to a significant proportion of the mutation load of iPSCs...
  47. ncbi request reprint Tbx20 dose-dependently regulates transcription factor networks required for mouse heart and motoneuron development
    Jun K Takeuchi
    Cardiovascular Research, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada
    Development 132:2463-74. 2005
    ..We conclude that Tbx20 is positioned at a critical node in transcription factor networks required for heart and motoneuron development where it dose-dependently regulates gene expression...
  48. ncbi request reprint Essential role of Mash-2 in extraembryonic development
    F Guillemot
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 371:333-6. 1994
    ..Mash-2 is the first transcription factor shown to play a critical part in the development of the mammalian trophoblast lineage...
  49. ncbi request reprint Parental origin-specific expression of Mash2 is established at the time of implantation with its imprinting mechanism highly resistant to genome-wide demethylation
    M Tanaka
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Mech Dev 87:129-42. 1999
    ..Our results suggest the possibility that a mechanism other than DNA methylation, such as allele-specific chromatin conformation, may be involved in maintenance of parental origin-specific expression of Mash2...
  50. ncbi request reprint Mash2 is expressed in oogenesis and preimplantation development but is not required for blastocyst formation
    J Rossant
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Mech Dev 73:183-91. 1998
    ..If Mash2 functions in other aspects of trophoblast development, it must act cooperatively with other factors...
  51. ncbi request reprint Improved embryonic stem cell technologies
    J S Draper
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Handb Exp Pharmacol . 2007
    ..This chapter will review both the history and recent technological progress that has been made in mouse ES cell derivation, genetic manipulation and the generation of ES cell-derived chimaeric animals...
  52. ncbi request reprint Mash2 acts cell autonomously in mouse spongiotrophoblast development
    M Tanaka
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Dev Biol 190:55-65. 1997
    ....
  53. ncbi request reprint Oligodendrogliomas result from the expression of an activated mutant epidermal growth factor receptor in a RAS transgenic mouse astrocytoma model
    Hao Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, USA
    Cancer Res 63:1106-13. 2003
    ....
  54. ncbi request reprint Mice with Cre recombinase activatable PDGF-C expression
    Hao Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genesis 32:181-3. 2002
  55. doi request reprint Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function
    Jing Jin
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Cell 151:384-99. 2012
    ..sFLT1 also regulates pericyte function in vessels outside of the kidney. Our findings demonstrate an autocrine function for sFLT1 to control pericyte behavior...
  56. ncbi request reprint Creation and use of a cre recombinase transgenic database
    Andras Nagy
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada
    Methods Mol Biol 530:365-78. 2009
    ....
  57. doi request reprint An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming
    Mathieu Gabut
    Banting and Best Department of Medical Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
    Cell 147:132-46. 2011
    ..These results reveal a pivotal role for an AS event in the regulation of pluripotency through the control of critical ESC-specific transcriptional programs...
  58. doi request reprint Cartilage tissue formation using redifferentiated passaged chondrocytes in vitro
    Nazish Ahmed
    CIHR BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, University of Toronto, Toronto, Canada
    Tissue Eng Part A 15:665-73. 2009
    ....
  59. doi request reprint Copy number variation and selection during reprogramming to pluripotency
    Samer M Hussein
    Samuel Lunenfeld Research Institute, Toronto, Ontario M5T 3H7, Canada
    Nature 471:58-62. 2011
    ..Remarkably, expansion of human iPS cells in culture selects rapidly against mutated cells, driving the lines towards a genetic state resembling human ES cells...
  60. ncbi request reprint Supplementation-dependent differences in the rates of embryonic stem cell self-renewal, differentiation, and apoptosis
    Sowmya Viswanathan
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Room 407, 4 Taddle Creek Road, Toronto, Ontario M5S 3G9, Canada
    Biotechnol Bioeng 84:505-17. 2003
    ..Our findings therefore have important applications in engineering stem cell culture systems to predictably generate desired stem cells or their derivatives for various regenerative therapies...
  61. ncbi request reprint Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity
    L Lefebvre
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada
    Nat Genet 27:257-8. 2001
    ..The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse...
  62. doi request reprint Secondary cell reprogramming systems: as years go by
    Andras Nagy
    Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada Institute of Medical Science, University of Toronto, Toronto, Ontario M5T 3H7, Canada Department of Obstetrics and Gynaecology, University of Toronto, Toronto, Ontario, Canada Electronic address
    Curr Opin Genet Dev 23:534-9. 2013
    ..This review outlines the key components of the 2°RS, its recent development and applications that have led to a deeper understanding of the 'magic' of induced cell fate changes...
  63. doi request reprint Concise review: Embryonic stem cells versus induced pluripotent stem cells: the game is on
    Mira C Puri
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Stem Cells 30:10-4. 2012
    ..Such comparative studies will be crucial for determining the more suitable cell type for future stem cell-based therapies for human degenerative diseases...
  64. doi request reprint Multifaceted role of vascular endothelial growth factor signaling in adult tissue physiology: an emerging concept with clinical implications
    Hoon Ki Sung
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Curr Opin Hematol 17:206-12. 2010
    ..This review will focus on the role of the VSP for proper function of many organs in adult life...
  65. pmc Niche-mediated control of human embryonic stem cell self-renewal and differentiation
    Raheem Peerani
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    EMBO J 26:4744-55. 2007
    ..Our results illustrate, for the first time, a role for Smad1 in the integration of spatial information and in the niche-size-dependent control of hESC self-renewal and differentiation...
  66. pmc Local acting Sticky-trap inhibits vascular endothelial growth factor dependent pathological angiogenesis in the eye
    Iacovos P Michael
    Lunenfeld Tanenbaum Research Institute Mount Sinai Hospital, Toronto, ON, Canada
    EMBO Mol Med 6:604-23. 2014
    ..These unique pharmacological characteristics of Sticky-trap could allow for safe treatment of pathological angiogenesis in patients with diabetic retinopathy and retinopathy of pre-maturity. ..
  67. ncbi request reprint Glycogen synthase kinase 3alpha-specific regulation of murine hepatic glycogen metabolism
    Katrina MacAulay
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada
    Cell Metab 6:329-37. 2007
    ..We conclude that GSK-3 isoforms exhibit tissue-specific physiological functions and that GSK-3alpha KO mice are insulin sensitive, reinforcing the potential of GSK-3 as a therapeutic target for type II diabetes...
  68. pmc Exogenous Mtv-7 superantigen transgene expression in major histocompatibility complex class II I-E- mice reconstituted with embryonic stem cell-derived hematopoietic stem cells
    C A Chambers
    Division of Neurobiology and Molecular Immunology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Proc Natl Acad Sci U S A 91:1138-42. 1994
    ....
  69. ncbi request reprint Embryonic development is disrupted by modest increases in vascular endothelial growth factor gene expression
    L Miquerol
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
    Development 127:3941-6. 2000
    ..In addition, aberrant coronary development was characterized by formation of oversized epicardial vessels, apparently through vasculogenesis. We infer that embryonic survival requires a narrow window of VEGF-A expression...
  70. ncbi request reprint The mouse Pdgfc gene: dynamic expression in embryonic tissues during organogenesis
    H Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Ontario, M5G 1X5, Toronto, Canada
    Mech Dev 96:209-13. 2000
    ..Contrary to PDGF-A, Pdgfc is not expressed in the central nervous system, except in the cerebellum, and neurogenic derivatives of the neural crest cells. Pdgfc is also absent from the heart and the vascular endothelium..
  71. ncbi request reprint ES cell-mediated conditional transgenesis
    M Gertsenstein
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Methods Mol Biol 185:285-307. 2002
  72. ncbi request reprint Astrocyte-specific expression of activated p21-ras results in malignant astrocytoma formation in a transgenic mouse model of human gliomas
    H Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5 Canada
    Cancer Res 61:3826-36. 2001
    ..Therefore, this transgenic mouse astrocytoma model recapitulates many of the molecular histopathological and growth characteristics of human malignant astrocytomas in a reproducible, germ-line-transmitted, and high-penetrance manner...
  73. pmc Expression and hypoxic regulation of angiopoietins in human astrocytomas
    H Ding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Neuro Oncol 3:1-10. 2001
    ..These data suggest that both VEGF and angiopoietins are involved in regulating tumor angiogenesis in human astrocytomas...
  74. ncbi request reprint Multiple developmental roles of VEGF suggested by a LacZ-tagged allele
    L Miquerol
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G1X5, Canada
    Dev Biol 212:307-22. 1999
    ..We believe that the LacZ-tagged allele we have generated offers a precise means of detecting VEGF expression under a variety of physiological and pathological conditions...
  75. pmc Heterogeneous vascular dependence of tumor cell populations
    J L Yu
    Molecular and Cellular Biology Research Program, Sunnybrook and Women's College Health Sciences Centre, 2075 Bayview Ave, Toronto, Ontario, Canada M4N 3M5
    Am J Pathol 158:1325-34. 2001
    ..This potential for selection of less vascular-dependent tumor cell variants throughout the course of disease progression may have important implications for the long-term efficacy of anti-angiogenic therapy...
  76. ncbi request reprint Gastrulation and angiogenesis, not endothelial specification, is sensitive to partial deficiency in vascular endothelial growth factor-a in mice
    Li Juan Duan
    Center for Vascular Biology, University of Connecticut Health Center, Farmington 06030, USA
    Biol Reprod 69:1852-8. 2003
    ....
  77. ncbi request reprint Insufficient VEGFA activity in yolk sac endoderm compromises haematopoietic and endothelial differentiation
    Annette Damert
    Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany
    Development 129:1881-92. 2002
    ..However, normal VEGFA expression in the yolk sac mesoderm alone is not sufficient for supporting the proper development of the early vascular and haematopoietic system...
  78. ncbi request reprint Transgenic targeting with regulatory elements of the human CD34 gene
    Hanna S Radomska
    Harvard Institutes of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:4410-9. 2002
    ....
  79. pmc Contrasting effects of VEGF gene disruption in embryonic stem cell-derived versus oncogene-induced tumors
    Alicia Viloria-Petit
    Molecular and Cellular Biology Research, Sunnybrook and Women s College Health Sciences Centre, University of Toronto, Ontario M4N 3M5, Canada
    EMBO J 22:4091-102. 2003
    ....
  80. ncbi request reprint SCL interacts with VEGF to suppress apoptosis at the onset of hematopoiesis
    Richard Martin
    Laboratory of Hematopoiesis and Leukemia, Clinical Research Institute of Montreal, Montreal, Canada
    Development 131:693-702. 2004
    ..This indicates that SCL functions downstream of VEGF to ensure an expansion of the hematopoietic compartment...
  81. pmc Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2
    Kotaro Takeda
    Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030 3501, USA
    Mol Cell Biol 26:8336-46. 2006
    ..Taken together, these data indicate that among all three PHD proteins, PHD2 is uniquely essential during mouse embryogenesis...
  82. ncbi request reprint Phenotypic analyses of mouse embryos with ubiquitous expression of Oct4: effects on mid-hindbrain patterning and gene expression
    Veronica Ramos-Mejia
    Departamento de Fisiologia Molecular y Genetica del Desarrollo, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    Dev Dyn 232:180-90. 2005
    ..Our observations support the hypothesis that Oct4, like zebrafish Pou2, has a conserved function during early brain patterning in mouse...
  83. pmc Autocrine VEGF signaling is required for vascular homeostasis
    Sunyoung Lee
    Department of Molecular, Cellular and Developmental Biology, Jonsson Comprehensive Cancer Center and Molecular Biology Institute, UCLA, Los Angeles, CA 90095, USA
    Cell 130:691-703. 2007
    ..These results reveal a cell-autonomous VEGF signaling pathway that holds significance for vascular homeostasis but is dispensable for the angiogenic cascade...
  84. pmc Epigenetic and phenotypic consequences of a truncation disrupting the imprinted domain on distal mouse chromosome 7
    Rosemary Oh
    Department of Medical Genetics, Life Sciences Centre, Molecular Epigenetics Group, 5503 2350 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia, Canada
    Mol Cell Biol 28:1092-103. 2008
    ..Thus, all the imprinted genes located in the region and required for normal development are silenced by an IC2-dependent mechanism on the paternal allele...
  85. pmc Vascular endothelial growth factor controls neuronal migration and cooperates with Sema3A to pattern distinct compartments of the facial nerve
    Quenten Schwarz
    Department of Cell Biology, Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom
    Genes Dev 18:2822-34. 2004
    ..These observations demonstrate, for the first time, that VEGF contributes to neuronal patterning in vivo, and that different compartments of one cell can be co-ordinately patterned by structurally distinct ligands for a shared receptor...
  86. pmc Oct4 is required for primordial germ cell survival
    James Kehler
    Germline Development, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania, 382 W Street Road, Kennett Square, Pennsylvania 19348, USA
    EMBO Rep 5:1078-83. 2004
    ..These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline...
  87. pmc The knockout mouse project
    Christopher P Austin
    National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA
    Nat Genet 36:921-4. 2004
    ..It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain...
  88. pmc Early restriction of peripheral and proximal cell lineages during formation of the lung
    Anne Karina T Perl
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    Proc Natl Acad Sci U S A 99:10482-7. 2002
    ....
  89. ncbi request reprint Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
    Oluseun Adewumi
    UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    Nat Biotechnol 25:803-16. 2007
    ..Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected...
  90. ncbi request reprint Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH gene
    J Henri Bayle
    Howard Hughes Medical Institute and the Department Pathology, Beckman Center for Molecular and Genetic Medicine, Stanford University, Stanford, California 94305, USA
    J Biol Chem 277:28884-91. 2002
    ..DCoH function as it pertains to HNF1 activity appears to be partially complemented by a newly identified homolog, DCoH2...
  91. ncbi request reprint Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer
    Malcolm Logan
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genesis 33:77-80. 2002
    ..Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb...
  92. ncbi request reprint Plasma extravasation induced by dietary supplemented histamine in histamine-free mice
    Hiroshi Ohtsu
    Department of Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Eur J Immunol 32:1698-708. 2002
    ..Therefore, among the allergic skin reactions, histamine is revealed to be an important mediator especially for the plasma extravasation in an immediate-type allergy model...
  93. ncbi request reprint Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice
    Yoshio Hori
    Departments of Cellular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan
    J Immunol 169:1978-83. 2002
    ..coli infectious peritonitis model, causing a delay in the elimination of bacteria. This also raised the possibility of the use of antihistamine drugs for bacterial infection...
  94. ncbi request reprint Ectopic expression of KitD814Y in spermatids of transgenic mice, interferes with sperm morphogenesis
    Denhi Schnabel
    Departamento de Fisiologia Molecular y Genetica del Desarrollo, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62271, Mexico
    Dev Dyn 233:29-40. 2005
    ..Our observations suggest that PLCgamma-1 activity could be involved in the shaping of spermatozoa...
  95. pmc ARS2 is a conserved eukaryotic gene essential for early mammalian development
    Michael D Wilson
    Centre for Biomedical Research, University of Victoria, P O Box 3020 Station CSC, Victoria, BC, Canada
    Mol Cell Biol 28:1503-14. 2008
    ..The analysis of data from several independent protein-protein interaction studies in mammals, combined with functional studies of its Arabidopsis ortholog, SERRATE, suggests that this essential process is related to RNA metabolism...
  96. ncbi request reprint "Agouti NOD": identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells
    Jing Chen
    The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609 1500, USA
    Mamm Genome 16:775-83. 2005
    ..CBALs- Tyr(+)/Lt blastocysts produced approximately 50% live-born mice, of which approximately 11% were chimeric. Presumably because of high genomic instability, no germline transmission was observed...
  97. doi request reprint Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells
    Michael D O'Connor
    Terry Fox Laboratory, 675 West 10th Avenue, Vancouver, British Columbia, Canada V5Z 1L3
    Stem Cells 26:1109-16. 2008
    ..Its use under standardized assay conditions should enhance future elucidation of the mechanisms that regulate hESC propagation and their early differentiation...
  98. pmc Activation of beta-catenin signaling programs embryonic epidermis to hair follicle fate
    Yuhang Zhang
    Department of Dermatology and Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Development 135:2161-72. 2008
    ..Controlled downregulation of beta-catenin signaling is required for normal placode patterning within embryonic ectoderm, hair follicle downgrowth, and adoption of the full range of follicular fates...
  99. doi request reprint Functional immobilization of signaling proteins enables control of stem cell fate
    Kristin Alberti
    Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Hohe Str 6, D 01069 Dresden, Germany
    Nat Methods 5:645-50. 2008
    ..The introduced method allows for the robust investigation of cell fate responses from interface-immobilized ligands...
  100. pmc The gut microbiota as an environmental factor that regulates fat storage
    Fredrik Backhed
    Center for Genome Sciences and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:15718-23. 2004
    ..Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AY 667702--AY 668946)...
  101. pmc Disruption of the endocytic protein HIP1 results in neurological deficits and decreased AMPA receptor trafficking
    Martina Metzler
    Centre for Molecular Medicine and Therapeutics, Childrens and Womens Hospital, Department of Medical Genetics, University of British Columbia, Vancouver, BC, V5Z 4H4
    EMBO J 22:3254-66. 2003
    ..Together, these data provide strong evidence that HIP1 regulates AMPA receptor trafficking in the central nervous system through its function in clathrin-mediated endocytosis...

Research Grants6

  1. New Directions in Mutagenized ES Cell Libraries
    Andras Nagy; Fiscal Year: 2001
    ..Both strategies will be developed first in existing 129 ES cells, but will be transferred to newly derived C57BL/6 cell lines, as soon as they are validated, to provide mutations on the B6 'gold standard' background. ..
  2. New Directions in Mutagenized ES Cell Libraries
    Andras Nagy; Fiscal Year: 2003
    ..Both strategies will be developed first in existing 129 ES cells, but will be transferred to newly derived C57BL/6 cell lines, as soon as they are validated, to provide mutations on the B6 'gold standard' background. ..
  3. New Directions in Mutagenized ES Cell Libraries
    Andras Nagy; Fiscal Year: 2002
    ..Both strategies will be developed first in existing 129 ES cells, but will be transferred to newly derived C57BL/6 cell lines, as soon as they are validated, to provide mutations on the B6 'gold standard' background. ..
  4. Contribution to the completion of Comprehensive Mouse Knockout Resource
    Andras Nagy; Fiscal Year: 2007
    ..This aspect will ensure the most cost effective methods are applied at each step along the way to completing the core goals of the KOMP. ..
  5. Contribution to the completion of Comprehensive Mouse Knockout Resource
    Andras Nagy; Fiscal Year: 2007
    ..This aspect will ensure the most cost effective methods are applied at each step along the way to completing the core goals of the KOMP. ..