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Species | L RaptisSummaryAffiliation: Queen's University Country: Canada Publications
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Publications
Cellular ras gene activity is required for full neoplastic transformation by the large tumor antigen of SV40L Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Cell Growth Differ 8:891-901. 1997..In addition, SVLT expression in C3H10T1/2 cells led to increased c-Ras activity, as determined by an increase in the Ras-bound GTP/GTP + GDP ratio. These results suggest that c-Ras is required for full neoplastic transformation by SVLT...- v-Ras and v-Raf block differentiation of transformable C3H10T1/2-derived preadipocytes at lower levels than required for neoplastic transformationL Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Exp Cell Res 235:188-97. 1997..Consistent with its established role as a downstream effector of Ras, v-Raf expression mirrored the v-Ras effects upon adipocytic differentiation and transformation... - Specific inhibition of growth factor-stimulated extracellular signal-regulated kinase 1 and 2 activation in intact cells by electroporation of a growth factor receptor-binding protein 2-Src homology 2 binding peptideL Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Cell Growth Differ 11:293-303. 2000..These results demonstrate the potential of the in situ electroporation approach described here in the study of the coupling of activated receptor tyrosine kinases to the ERK1/2 cascade... - In situ electroporation of large numbers of cells using minimal volumes of materialLeda Raptis
Department of Microbiology and Immunology, Queen's University, Kingston Ontario, Canada K7L3N6
Anal Biochem 317:124-8. 2003 - Ras(leu61) blocks differentiation of transformable 3T3 L1 and C3H10T1/2-derived preadipocytes in a dose- and time-dependent mannerL Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Cell Growth Differ 8:11-21. 1997..Consistent with its established role as a downstream effector of Ras, v-Raf expression mirrored the Rasleu61 effects on adipocytic differentiation and transformation... 
Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acidG Li
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Br J Cancer 80:676-84. 1999..Identification of the nature of that linkage may lead to new approaches to cancer therapy...- Adenovirus E1A requires c-Ras for full neoplastic transformation or suppression of differentiation of murine preadipocytesJ Cao
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Mol Carcinog 46:284-302. 2007.... - Transfection techniques affecting Stat3 activity levelsR Arulanandam
Department of Microbiology and Immunology and Department of Pathology and Molecular Medicine and Cancer Research Center, Queen's University, Kingston, Ont, Canada K7L 3N6
Anal Biochem 338:83-9. 2005..These results indicate that caution is required in the interpretation of results with regard to activity of Stat3 following certain commonly used transient transfection regimens... - Cell-to-cell adhesion modulates Stat3 activity in normal and breast carcinoma cellsAdina Vultur
Department of Microbiology, Queen s University, Kingston, Ontario, Canada K7L 3N6
Oncogene 23:2600-16. 2004..More importantly, our results suggest that Stat3 activity is upregulated during the confluence-mediated growth arrest by a signalling mechanism that requires JAKs... 
Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communicationLeda Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Methods Mol Biol 423:173-89. 2008....- Beyond structure, to survival: activation of Stat3 by cadherin engagementLeda Raptis
Department of Microbiology and Immunology, and Cancer Research Institute, Queen s University, Kingston, ON K7L 3N6
Biochem Cell Biol 87:835-43. 2009..It is clear that at any given time the total Stat3 activity levels in a cell are the sum of the effects of cell to cell adhesion plus the conventional Stat3 activating factors present... - TAZ is a novel oncogene in non-small cell lung cancerZ Zhou
Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario, Canada
Oncogene 30:2181-6. 2011..These results indicate that TAZ is an oncogene and has an important role in tumorigenicity of NSCLC cells. Therefore, TAZ may present a novel target for the future diagnosis, prognosis and therapy of lung cancer... - Electrode assemblies used for electroporation of cultured cellsLeda Raptis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Methods Mol Biol 423:61-76. 2008..An assembly is also described for the electroporation of sensitive cells without the use of an upper electrode... - Examination of gap junctional, intercellular communication by in situ electroporation on two co-planar indium-tin oxide electrodesAikaterini Anagnostopoulou
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, K7L3N6, Canada
Mol Oncol 1:226-31. 2007..This technique can also be used for the introduction of other non-permeant molecules such as peptides or siRNA, followed by examination of the cellular phenotype or gene expression levels in situ... - The role of Hsp90N, a new member of the Hsp90 family, in signal transduction and neoplastic transformationNicholas Grammatikakis
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6
J Biol Chem 277:8312-20. 2002..With the recent finding that p50(cdc37) is tumorigenic in transgenic mice, these results reinforce the intriguing observation that the family of heat shock proteins represents a novel class of molecules with oncogenic potential... - Activated Rac1 requires gp130 for Stat3 activation, cell proliferation and migrationRozanne Arulanandam
Departments of Microbiology and Immunology and Pathology and Molecular Medicine, and Queen s University Cancer Institute, Queen s University, Botterell Hall, Rm 713, Kingston, Ontario, Canada K7L3N6
Exp Cell Res 316:875-86. 2010.... - Cadherin-cadherin engagement promotes cell survival via Rac1/Cdc42 and signal transducer and activator of transcription-3Rozanne Arulanandam
Department of Microbiology and Immunology, Department of Pathology and Molecular Medicine, and Cancer Research Institute, Queen s University, Ontario, Canada K7L 3N6
Mol Cancer Res 7:1310-27. 2009..Mol Cancer Res 2009;7(8):1310-27)... - Differential effects of Stat3 inhibition in sparse vs confluent normal and breast cancer cellsAikaterini Anagnostopoulou
Department of Microbiology, Queen s University, Kingston, Ont, Canada K7L3N6
Cancer Lett 242:120-32. 2006..In normal cells on the other hand, Stat3 inhibition at post-confluence caused apoptosis while in sparsely growing cells it induced merely a growth retardation... - Gap junctional intercellular communication in cells isolated from urethane-induced tumors in A/J miceAdina Vultur
Department of Microbiology, Queen's University, Kingston, Ontario, Canada
DNA Cell Biol 22:33-40. 2003..Propagation of tumor cells in culture induces additional alterations that can lead to gap junction closure... - Synthesis, characterization and Stat3 inhibitory properties of the prototypical platinum(IV) anticancer drug, [PtCl3(NO2)(NH3)2] (CPA-7)Shalyn L Littlefield
Department of Chemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
Inorg Chem 47:2798-804. 2008..The freshly prepared drug is obtained as a single isomer which may in fact be fac- or mer-[PtCl3(NO2)(NH3)2], but recrystallization resulted in a disordered crystal containing approximately equal amounts of the two geometric isomers... - In situ electroporation of radioactive compounds into adherent cellsEvangelia Tomai
Department of Microbiology, Queen's University, Kingston, Ontario, Canada
DNA Cell Biol 22:339-46. 2003.... - The simian virus 40 large tumor antigen activates cSrc and requires cSrc for full neoplastic transformationRozanne Arulanandam
Department of Microbiology and Immunology, Queen s University, Botterell Hall, Rm 713, Kingston, Ontario K7L3N6, Canada
Anticancer Res 30:47-53. 2010..To investigate the role of the cellular protooncogene product, cSrc, in neoplastic transformation by the large tumor antigen of simian virus 40 (TAg), the ability of TAg to increase cSrc activity was examined... - Housekeeping genes; expression levels may change with density of cultured cellsSamantha Greer
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
J Immunol Methods 355:76-9. 2010..On the other hand the levels of heat-shock protein-90 and beta-actin remained unchanged at a wide range of cell densities, making these proteins into more reliable loading controls... - Differential effects of c-Ras upon transformation, adipocytic differentiation, and apoptosis mediated by the simian virus 40 large tumor antigenJun Cao
Department of Microbiology, Queen s University, Kingston, Ont, Canada
Biochem Cell Biol 85:32-48. 2007.... - Adenovirus-5 E1A suppresses differentiation of 3T3 L1 preadipocytes at lower levels than required for induction of apoptosisJun Cao
Departments of Microbiology and Immunology and Pathology, and Cancer Research Center, Queen's University, Kingston, Ontario, Canada
Mol Carcinog 43:38-50. 2005..These data reveal a dissociation between E1A signals leading to transformation, suppression of differentiation and induction of apoptosis, based on levels of expression... - Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigenAdina Vultur
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
Mol Biol Cell 16:3832-46. 2005..Taken together, our results suggest that Stat3 is an important component of a pathway emanating from TAg and leading to neoplastic conversion...