L Raptis

Summary

Affiliation: Queen's University
Country: Canada

Publications

  1. ncbi request reprint Cellular ras gene activity is required for full neoplastic transformation by the large tumor antigen of SV40
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 8:891-901. 1997
  2. ncbi request reprint v-Ras and v-Raf block differentiation of transformable C3H10T1/2-derived preadipocytes at lower levels than required for neoplastic transformation
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Exp Cell Res 235:188-97. 1997
  3. ncbi request reprint Specific inhibition of growth factor-stimulated extracellular signal-regulated kinase 1 and 2 activation in intact cells by electroporation of a growth factor receptor-binding protein 2-Src homology 2 binding peptide
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 11:293-303. 2000
  4. ncbi request reprint In situ electroporation of large numbers of cells using minimal volumes of material
    Leda Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston Ontario, Canada K7L3N6
    Anal Biochem 317:124-8. 2003
  5. ncbi request reprint Ras(leu61) blocks differentiation of transformable 3T3 L1 and C3H10T1/2-derived preadipocytes in a dose- and time-dependent manner
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 8:11-21. 1997
  6. pmc Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid
    G Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Br J Cancer 80:676-84. 1999
  7. ncbi request reprint Adenovirus E1A requires c-Ras for full neoplastic transformation or suppression of differentiation of murine preadipocytes
    J Cao
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Carcinog 46:284-302. 2007
  8. ncbi request reprint Transfection techniques affecting Stat3 activity levels
    R Arulanandam
    Department of Microbiology and Immunology and Department of Pathology and Molecular Medicine and Cancer Research Center, Queen s University, Kingston, Ont, Canada K7L 3N6
    Anal Biochem 338:83-9. 2005
  9. doi request reprint Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communication
    Leda Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Methods Mol Biol 423:173-89. 2008
  10. ncbi request reprint Cell-to-cell adhesion modulates Stat3 activity in normal and breast carcinoma cells
    Adina Vultur
    Department of Microbiology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Oncogene 23:2600-16. 2004

Collaborators

  • J Turkson
  • M J Corbley
  • S Chevalier
  • G Li
  • J Yang
  • Y Lu
  • M S Tsao
  • Nicholas Grammatikakis
  • X Yang
  • G M Ross
  • Bruce E Elliott
  • Lionel Larue
  • Poh Gek Forkert
  • Rozanne Arulanandam
  • Adina Vultur
  • Jun Cao
  • Aikaterini Anagnostopoulou
  • R Arulanandam
  • Mulu Geletu
  • Evangelia Tomai
  • M Geletu
  • Richard Jove
  • Z Zhou
  • Samantha Greer
  • Helene Feracci
  • Shalyn L Littlefield
  • J Cao
  • A Vultur
  • H Feracci
  • B Saez
  • Y Hao
  • N Liu
  • Rice Honeywell
  • Peter F Truesdell
  • Esther Carefoot
  • Michael C Baird
  • Kevin Firth
  • A Anagnostopoulou
  • Aikaterini Anagnostopoulu
  • Joon S Kim
  • Matthew Glenn
  • Andrew D Hamilton
  • Thomas Preston
  • Guilian Niu
  • Keivan Zandi
  • Katarzyna Jaronczyk
  • Peter Greer
  • Andrew Craig
  • Alvin M Malkinson
  • Valerie Balboa
  • Kevin L Firth
  • Heather L Brownell
  • Katherine Peebles
  • Tina Hsu

Detail Information

Publications27

  1. ncbi request reprint Cellular ras gene activity is required for full neoplastic transformation by the large tumor antigen of SV40
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 8:891-901. 1997
    ..In addition, SVLT expression in C3H10T1/2 cells led to increased c-Ras activity, as determined by an increase in the Ras-bound GTP/GTP + GDP ratio. These results suggest that c-Ras is required for full neoplastic transformation by SVLT...
  2. ncbi request reprint v-Ras and v-Raf block differentiation of transformable C3H10T1/2-derived preadipocytes at lower levels than required for neoplastic transformation
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Exp Cell Res 235:188-97. 1997
    ..Consistent with its established role as a downstream effector of Ras, v-Raf expression mirrored the v-Ras effects upon adipocytic differentiation and transformation...
  3. ncbi request reprint Specific inhibition of growth factor-stimulated extracellular signal-regulated kinase 1 and 2 activation in intact cells by electroporation of a growth factor receptor-binding protein 2-Src homology 2 binding peptide
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 11:293-303. 2000
    ..These results demonstrate the potential of the in situ electroporation approach described here in the study of the coupling of activated receptor tyrosine kinases to the ERK1/2 cascade...
  4. ncbi request reprint In situ electroporation of large numbers of cells using minimal volumes of material
    Leda Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston Ontario, Canada K7L3N6
    Anal Biochem 317:124-8. 2003
  5. ncbi request reprint Ras(leu61) blocks differentiation of transformable 3T3 L1 and C3H10T1/2-derived preadipocytes in a dose- and time-dependent manner
    L Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Cell Growth Differ 8:11-21. 1997
    ..Consistent with its established role as a downstream effector of Ras, v-Raf expression mirrored the Rasleu61 effects on adipocytic differentiation and transformation...
  6. pmc Rodent fibroblast model for studies of response of malignant cells to exogenous 5-aminolevulinic acid
    G Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Br J Cancer 80:676-84. 1999
    ..Identification of the nature of that linkage may lead to new approaches to cancer therapy...
  7. ncbi request reprint Adenovirus E1A requires c-Ras for full neoplastic transformation or suppression of differentiation of murine preadipocytes
    J Cao
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Carcinog 46:284-302. 2007
    ....
  8. ncbi request reprint Transfection techniques affecting Stat3 activity levels
    R Arulanandam
    Department of Microbiology and Immunology and Department of Pathology and Molecular Medicine and Cancer Research Center, Queen s University, Kingston, Ont, Canada K7L 3N6
    Anal Biochem 338:83-9. 2005
    ..These results indicate that caution is required in the interpretation of results with regard to activity of Stat3 following certain commonly used transient transfection regimens...
  9. doi request reprint Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communication
    Leda Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Methods Mol Biol 423:173-89. 2008
    ....
  10. ncbi request reprint Cell-to-cell adhesion modulates Stat3 activity in normal and breast carcinoma cells
    Adina Vultur
    Department of Microbiology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Oncogene 23:2600-16. 2004
    ..More importantly, our results suggest that Stat3 activity is upregulated during the confluence-mediated growth arrest by a signalling mechanism that requires JAKs...
  11. doi request reprint Beyond structure, to survival: activation of Stat3 by cadherin engagement
    Leda Raptis
    Department of Microbiology and Immunology, and Cancer Research Institute, Queen s University, Kingston, ON K7L 3N6
    Biochem Cell Biol 87:835-43. 2009
    ..It is clear that at any given time the total Stat3 activity levels in a cell are the sum of the effects of cell to cell adhesion plus the conventional Stat3 activating factors present...
  12. doi request reprint TAZ is a novel oncogene in non-small cell lung cancer
    Z Zhou
    Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario, Canada
    Oncogene 30:2181-6. 2011
    ..These results indicate that TAZ is an oncogene and has an important role in tumorigenicity of NSCLC cells. Therefore, TAZ may present a novel target for the future diagnosis, prognosis and therapy of lung cancer...
  13. doi request reprint Electrode assemblies used for electroporation of cultured cells
    Leda Raptis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Methods Mol Biol 423:61-76. 2008
    ..An assembly is also described for the electroporation of sensitive cells without the use of an upper electrode...
  14. doi request reprint Activated Rac1 requires gp130 for Stat3 activation, cell proliferation and migration
    Rozanne Arulanandam
    Departments of Microbiology and Immunology and Pathology and Molecular Medicine, and Queen s University Cancer Institute, Queen s University, Botterell Hall, Rm 713, Kingston, Ontario, Canada K7L3N6
    Exp Cell Res 316:875-86. 2010
    ....
  15. doi request reprint Cadherin-cadherin engagement promotes cell survival via Rac1/Cdc42 and signal transducer and activator of transcription-3
    Rozanne Arulanandam
    Department of Microbiology and Immunology, Department of Pathology and Molecular Medicine, and Cancer Research Institute, Queen s University, Ontario, Canada K7L 3N6
    Mol Cancer Res 7:1310-27. 2009
    ..Mol Cancer Res 2009;7(8):1310-27)...
  16. ncbi request reprint Examination of gap junctional, intercellular communication by in situ electroporation on two co-planar indium-tin oxide electrodes
    Aikaterini Anagnostopoulou
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, K7L3N6, Canada
    Mol Oncol 1:226-31. 2007
    ..This technique can also be used for the introduction of other non-permeant molecules such as peptides or siRNA, followed by examination of the cellular phenotype or gene expression levels in situ...
  17. ncbi request reprint The role of Hsp90N, a new member of the Hsp90 family, in signal transduction and neoplastic transformation
    Nicholas Grammatikakis
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6
    J Biol Chem 277:8312-20. 2002
    ..With the recent finding that p50(cdc37) is tumorigenic in transgenic mice, these results reinforce the intriguing observation that the family of heat shock proteins represents a novel class of molecules with oncogenic potential...
  18. doi request reprint Classical cadherins control survival through the gp130/Stat3 axis
    M Geletu
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Biochim Biophys Acta 1833:1947-59. 2013
    ....
  19. ncbi request reprint Differential effects of Stat3 inhibition in sparse vs confluent normal and breast cancer cells
    Aikaterini Anagnostopoulou
    Department of Microbiology, Queen s University, Kingston, Ont, Canada K7L3N6
    Cancer Lett 242:120-32. 2006
    ..In normal cells on the other hand, Stat3 inhibition at post-confluence caused apoptosis while in sparsely growing cells it induced merely a growth retardation...
  20. ncbi request reprint Gap junctional intercellular communication in cells isolated from urethane-induced tumors in A/J mice
    Adina Vultur
    Department of Microbiology, Queen s University, Kingston, Ontario, Canada
    DNA Cell Biol 22:33-40. 2003
    ..Propagation of tumor cells in culture induces additional alterations that can lead to gap junction closure...
  21. doi request reprint Housekeeping genes; expression levels may change with density of cultured cells
    Samantha Greer
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Immunol Methods 355:76-9. 2010
    ..On the other hand the levels of heat-shock protein-90 and beta-actin remained unchanged at a wide range of cell densities, making these proteins into more reliable loading controls...
  22. ncbi request reprint The simian virus 40 large tumor antigen activates cSrc and requires cSrc for full neoplastic transformation
    Rozanne Arulanandam
    Department of Microbiology and Immunology, Queen s University, Botterell Hall, Rm 713, Kingston, Ontario K7L3N6, Canada
    Anticancer Res 30:47-53. 2010
    ..To investigate the role of the cellular protooncogene product, cSrc, in neoplastic transformation by the large tumor antigen of simian virus 40 (TAg), the ability of TAg to increase cSrc activity was examined...
  23. ncbi request reprint In situ electroporation of radioactive compounds into adherent cells
    Evangelia Tomai
    Department of Microbiology, Queen s University, Kingston, Ontario, Canada
    DNA Cell Biol 22:339-46. 2003
    ....
  24. doi request reprint Synthesis, characterization and Stat3 inhibitory properties of the prototypical platinum(IV) anticancer drug, [PtCl3(NO2)(NH3)2] (CPA-7)
    Shalyn L Littlefield
    Department of Chemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Inorg Chem 47:2798-804. 2008
    ..The freshly prepared drug is obtained as a single isomer which may in fact be fac- or mer-[PtCl3(NO2)(NH3)2], but recrystallization resulted in a disordered crystal containing approximately equal amounts of the two geometric isomers...
  25. ncbi request reprint Differential effects of c-Ras upon transformation, adipocytic differentiation, and apoptosis mediated by the simian virus 40 large tumor antigen
    Jun Cao
    Department of Microbiology, Queen s University, Kingston, Ont, Canada
    Biochem Cell Biol 85:32-48. 2007
    ....
  26. ncbi request reprint Adenovirus-5 E1A suppresses differentiation of 3T3 L1 preadipocytes at lower levels than required for induction of apoptosis
    Jun Cao
    Departments of Microbiology and Immunology and Pathology, and Cancer Research Center, Queen s University, Kingston, Ontario, Canada
    Mol Carcinog 43:38-50. 2005
    ..These data reveal a dissociation between E1A signals leading to transformation, suppression of differentiation and induction of apoptosis, based on levels of expression...
  27. pmc Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen
    Adina Vultur
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Mol Biol Cell 16:3832-46. 2005
    ..Taken together, our results suggest that Stat3 is an important component of a pathway emanating from TAg and leading to neoplastic conversion...