Genomes and Genes
Affiliation: Public Health Agency of Canada
- In vitro and in vivo characterization of recombinant Ebola viruses expressing enhanced green fluorescent proteinHideki Ebihara
Department of Special Pathogens, International Research Center for Infectious Diseases, University of Tokyo, Tokyo, Japan
J Infect Dis 196:S313-22. 2007..These findings demonstrate that the incorporation of a foreign gene will attenuate ZEBOV in vivo but that these viruses still have potential for in vitro and in vivo research applications...
- Molecular determinants of Ebola virus virulence in miceHideki Ebihara
Institute of Medical Science, University of Tokyo, Tokyo, Japan
PLoS Pathog 2:e73. 2006..These findings suggest a critical role for overcoming the interferon-induced antiviral state in the pathogenicity of EBOV and offer new insights into the pathogenesis of EBOV infection...
- Tyro3 family-mediated cell entry of Ebola and Marburg virusesMasayuki Shimojima
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
J Virol 80:10109-16. 2006..Antibody to Axl inhibited infection of pseudotype viruses in a number of Axl-positive cell lines. These results implicate each Tyro3 family member as a cell entry factor in filovirus infection...
- Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entryMasayuki Shimojima
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
J Virol 86:2067-78. 2012..The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry...
- The role of reverse genetics systems in studying viral hemorrhagic feversHideki Ebihara
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Japan
Thromb Haemost 94:240-53. 2005..Here we review the existing reverse genetics systems for VHF causing viruses and discuss their use in studying viral replication, pathogenesis, and the development of antivirals and vaccines...
- Ebola virus matrix protein VP40 uses the COPII transport system for its intracellular transportSeiya Yamayoshi
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639, Japan
Cell Host Microbe 3:168-77. 2008..These findings identify a conserved intersection between a host pathway and filovirus replication, an intersection that can be targeted in the development of new antiviral drugs...
- Human macrophage C-type lectin specific for galactose and N-acetylgalactosamine promotes filovirus entryAyato Takada
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
J Virol 78:2943-7. 2004..We propose that filoviruses use different C-type lectins to gain cellular entry, depending on the cell type, and promote efficient viral replication...
- In vitro evaluation of antisense RNA efficacy against filovirus infection, by use of reverse geneticsAllison Groseth
National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada
J Infect Dis 196:S382-9. 2007..Therefore, we explored the application of minigenomes as screening tools to identify functional siRNA targets under biosafety level 2 conditions...
- Epitopes required for antibody-dependent enhancement of Ebola virus infectionAyato Takada
Department of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 060 0818, Japan
J Infect Dis 196:S347-56. 2007..These data suggest that GP lacking the ADE-inducing epitopes may increase the potential of GP as a vaccine antigen...
- The multimerization of hantavirus nucleocapsid protein depends on type-specific epitopesKumiko Yoshimatsu
Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo 060 8638, Japan
J Virol 77:943-52. 2003..Combined, the results indicate that several steps and regions are involved in multimerization of hantavirus NP...
- Use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization testMichiko Ogino
Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine, Sapporo 060 8638, Japan
Clin Diagn Lab Immunol 10:154-60. 2003..The stability of the pseudotype viruses allows distribution of the material to remote areas by using conventional cooling boxes for use as a diagnostic reagent...
- Protective efficacy of neutralizing antibodies against Ebola virus infectionAyato Takada
Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 060 0818, Japan
Vaccine 25:993-9. 2007..These results underscore the potential therapeutic utility of monoclonal antibodies for postexposure treatment of Ebola virus infections...
- Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplificationYohei Kurosaki
National Research Institute of Police Science, Kashiwa 277 0882, Japan
J Virol Methods 141:78-83. 2007..Furthermore, as this technique does not require sophisticated instrumentation, it seems very suitable for diagnosis in the field or laboratories in Ebola outbreak areas such as Central Africa...
- Infection of naive target cells with virus-like particles: implications for the function of ebola virus VP24Thomas Hoenen
Institut fur Virologie, Philipps Universitat Marburg, Marburg, Germany
J Virol 80:7260-4. 2006..Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex...
- Cell fusion activities of Hantaan virus envelope glycoproteinsMichiko Ogino
Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
J Virol 78:10776-82. 2004..This variability in fusion induction suggests the involvement of host cell factors in the process of cell membrane fusion...
- Generation of biologically contained Ebola virusesPeter Halfmann
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 105:1129-33. 2008....
- Assembly and budding of EbolavirusTakeshi Noda
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
PLoS Pathog 2:e99. 2006..These data form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus...