Vincent Poitout

Summary

Country: Canada

Publications

  1. pmc Characterization of the human pancreatic islet proteome by two-dimensional LC/MS/MS
    Thomas O Metz
    Biological Science Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA
    J Proteome Res 5:3345-54. 2006
  2. pmc Glucolipotoxicity: fuel excess and beta-cell dysfunction
    Vincent Poitout
    Montreal Diabetes Research Center, CR CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada H1W 4A4
    Endocr Rev 29:351-66. 2008
  3. pmc Glucolipotoxicity of the pancreatic beta cell
    Vincent Poitout
    Montreal Diabetes Research Center, CRCHUM, Department of Medicine, University of Montreal, Montreal, QC, Canada
    Biochim Biophys Acta 1801:289-98. 2010
  4. doi request reprint Modulating GPR40: therapeutic promise and potential in diabetes
    Vincent Poitout
    Montreal Diabetes Research Center, CRCHUM, and Department of Medicine, Universite de Montreal, Montreal, QC, Canada Electronic address
    Drug Discov Today 18:1301-8. 2013
  5. pmc Lipotoxicity impairs incretin signalling
    V Poitout
    Montreal Diabetes Research Center, CRCHUM, Technopole Angus, 2901 Rachel Est, Montreal, QC, Canada
    Diabetologia 56:231-3. 2013
  6. pmc Regulation of the insulin gene by glucose and fatty acids
    Vincent Poitout
    Department of Medicine, University of Montreal, Montreal, QC, Canada
    J Nutr 136:873-6. 2006
  7. pmc Glucolipotoxicity of the pancreatic beta-cell: myth or reality?
    Vincent Poitout
    Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, Canada, H1W 4A4
    Biochem Soc Trans 36:901-4. 2008
  8. pmc Phospholipid hydrolysis and insulin secretion: a step toward solving the Rubik's cube
    Vincent Poitout
    Montreal Diabetes Research Center, CR CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada
    Am J Physiol Endocrinol Metab 294:E214-6. 2008
  9. pmc Lack of preservation of insulin gene expression by a glucagon-like peptide 1 agonist or a dipeptidyl peptidase 4 inhibitor in an in vivo model of glucolipotoxicity
    Ghislaine Fontes
    Montreal Diabetes Research Center, CRCHUM, University of Montreal, QC, Canada
    Diabetes Res Clin Pract 87:322-8. 2010
  10. pmc Pioglitazone acutely reduces energy metabolism and insulin secretion in rats
    Julien Lamontagne
    Molecular Nutrition Unit and Montreal Diabetes Research Center at the Centre de Recherche du Centre Hospitalier de l Université de Montréal, Universite de Montreal, Montreal, Quebec, Canada
    Diabetes 62:2122-9. 2013

Research Grants

Collaborators

Detail Information

Publications42

  1. pmc Characterization of the human pancreatic islet proteome by two-dimensional LC/MS/MS
    Thomas O Metz
    Biological Science Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA
    J Proteome Res 5:3345-54. 2006
    ..The resulting peptide reference library provides a resource for future higher throughput and quantitative studies of islet biology...
  2. pmc Glucolipotoxicity: fuel excess and beta-cell dysfunction
    Vincent Poitout
    Montreal Diabetes Research Center, CR CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada H1W 4A4
    Endocr Rev 29:351-66. 2008
    ..Finally, we attempt to define the role of these phenomena in the natural history of beta-cell compensation, decompensation, and failure during the course of type 2 diabetes...
  3. pmc Glucolipotoxicity of the pancreatic beta cell
    Vincent Poitout
    Montreal Diabetes Research Center, CRCHUM, Department of Medicine, University of Montreal, Montreal, QC, Canada
    Biochim Biophys Acta 1801:289-98. 2010
    ..It is therefore likely that glucolipotoxicity contributes to beta-cell failure in type 2 diabetes as well as to the decline in beta-cell function observed after the onset of the disease...
  4. doi request reprint Modulating GPR40: therapeutic promise and potential in diabetes
    Vincent Poitout
    Montreal Diabetes Research Center, CRCHUM, and Department of Medicine, Universite de Montreal, Montreal, QC, Canada Electronic address
    Drug Discov Today 18:1301-8. 2013
    ..Much remains to be discovered regarding the biology of the receptor to inform the development of GPR40-based drugs. ..
  5. pmc Lipotoxicity impairs incretin signalling
    V Poitout
    Montreal Diabetes Research Center, CRCHUM, Technopole Angus, 2901 Rachel Est, Montreal, QC, Canada
    Diabetologia 56:231-3. 2013
    ..These novel findings provide convincing support for the notion that restoring normal circulating lipid levels in type 2 diabetes might help improve the efficacy of incretin-based therapies...
  6. pmc Regulation of the insulin gene by glucose and fatty acids
    Vincent Poitout
    Department of Medicine, University of Montreal, Montreal, QC, Canada
    J Nutr 136:873-6. 2006
    ..Glucotoxicity and lipotoxicity have common targets, which makes their combination particularly harmful to insulin gene expression and beta-cell function in type 2 diabetes...
  7. pmc Glucolipotoxicity of the pancreatic beta-cell: myth or reality?
    Vincent Poitout
    Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, Canada, H1W 4A4
    Biochem Soc Trans 36:901-4. 2008
    ....
  8. pmc Phospholipid hydrolysis and insulin secretion: a step toward solving the Rubik's cube
    Vincent Poitout
    Montreal Diabetes Research Center, CR CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada
    Am J Physiol Endocrinol Metab 294:E214-6. 2008
  9. pmc Lack of preservation of insulin gene expression by a glucagon-like peptide 1 agonist or a dipeptidyl peptidase 4 inhibitor in an in vivo model of glucolipotoxicity
    Ghislaine Fontes
    Montreal Diabetes Research Center, CRCHUM, University of Montreal, QC, Canada
    Diabetes Res Clin Pract 87:322-8. 2010
    ..Prolonged exposure of pancreatic beta-cells to elevated levels of glucose and fatty acids adversely affects insulin secretion and gene expression...
  10. pmc Pioglitazone acutely reduces energy metabolism and insulin secretion in rats
    Julien Lamontagne
    Molecular Nutrition Unit and Montreal Diabetes Research Center at the Centre de Recherche du Centre Hospitalier de l Université de Montréal, Universite de Montreal, Montreal, Quebec, Canada
    Diabetes 62:2122-9. 2013
    ..The results suggest that pioglitazone has direct metabolic deceleration effects on the β-cell that may contribute to its capacity to lower insulinemia and antidiabetic action...
  11. pmc Deletion of GPR40 impairs glucose-induced insulin secretion in vivo in mice without affecting intracellular fuel metabolism in islets
    Thierry Alquier
    Montreal Diabetes Research Center, Research Centre of the Montréal University Hospital, University of Montreal, Montreal, QC, Canada
    Diabetes 58:2607-15. 2009
    ..This study was aimed to ascertain whether GPR40 controls insulin secretion in vivo and modulates intracellular fuel metabolism in islets...
  12. pmc The fatty acid receptor GPR40 plays a role in insulin secretion in vivo after high-fat feeding
    Melkam Kebede
    Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Montreal, Canada
    Diabetes 57:2432-7. 2008
    ..Glucose tolerance, insulin tolerance, and insulin secretion in response to glucose and Intralipid were assessed during the course of the diet period...
  13. pmc Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3β in pancreatic β-cells
    Meriem Semache
    Montreal Diabetes Research Center, CRCHUM, Quebec City H1W4A4, Canada
    J Biol Chem 288:24825-33. 2013
    ..We conclude that PASK phosphorylates and inactivates GSK3β, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3β-mediated PDX-1 protein degradation in pancreatic β-cells. ..
  14. pmc Pioglitazone acutely reduces insulin secretion and causes metabolic deceleration of the pancreatic beta-cell at submaximal glucose concentrations
    Julien Lamontagne
    Department of Nutrition and Biochemistry, Molecular Nutrition Unit and Montreal Diabetes Research Center, Universite de Montreal, Montreal, Quebec, Canada
    Endocrinology 150:3465-74. 2009
    ..We suggest that AMPK activation and the metabolic deceleration of the beta-cell caused by Pio contribute to its known effects to reduce hyperinsulinemia and preserve beta-cell function and act as an antidiabetic agent...
  15. pmc Cyclical and alternating infusions of glucose and intralipid in rats inhibit insulin gene expression and Pdx-1 binding in islets
    Derek K Hagman
    DVM, Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, H1W 4A4, Canada
    Diabetes 57:424-31. 2008
    ..Whether such a phenomenon also occurs in vivo is unknown. Our objective was therefore to ascertain whether chronic nutrient oversupply inhibits insulin gene expression in vivo...
  16. pmc Lipopolysaccharides impair insulin gene expression in isolated islets of Langerhans via Toll-Like Receptor-4 and NF-κB signalling
    Julie Amyot
    Montreal Diabetes Research Center, Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Montreal, Quebec, Canada
    PLoS ONE 7:e36200. 2012
    ..In this study, we tested the hypothesis that LPS alters insulin gene expression via TLR4 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in islets...
  17. pmc Binding of activating transcription factor 6 to the A5/Core of the rat insulin II gene promoter does not mediate its transcriptional repression
    Julie Amyot
    Montreal Diabetes Research Center, Centre de recherche du Centre hospitalier de l Université de Montréal CRCHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec H1W 4A4, Canada
    J Mol Endocrinol 47:273-83. 2011
    ..Therefore, although ATF6 binds directly to the A5/Core of the rat insulin II gene promoter, this direct binding does not appear to contribute to its repressive activity...
  18. pmc Glucose activates free fatty acid receptor 1 gene transcription via phosphatidylinositol-3-kinase-dependent O-GlcNAcylation of pancreas-duodenum homeobox-1
    Melkam Kebede
    Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, and Department of Medicine, University of Montreal, Montreal, QC, Canada H3T 1J4
    Proc Natl Acad Sci U S A 109:2376-81. 2012
    ..These observations reveal a unique mechanism by which glucose metabolism regulates the function of transcription factors in the nucleus to induce gene expression...
  19. pmc Involvement of Per-Arnt-Sim Kinase and extracellular-regulated kinases-1/2 in palmitate inhibition of insulin gene expression in pancreatic beta-cells
    Ghislaine Fontes
    Montreal Diabetes Research Center, CRCHUM, University of Montreal, Quebec, Canada
    Diabetes 58:2048-58. 2009
    ..This study aimed to ascertain the role of extracellular-regulated kinases (ERKs)1/2, protein kinase B (PKB), and Per-Arnt-Sim kinase (PASK) in palmitate inhibition of insulin gene expression in pancreatic beta-cells...
  20. doi request reprint Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Bader Zarrouki
    Montreal Diabetes Research Center, University of Montreal, Montreal, Quebec, Canada
    Diabetes 63:982-93. 2014
    ..We conclude that chronic nutrient excess promotes β-cell mass expansion via a pathway that involves EGFR signaling, mTOR activation, and FOXM1-mediated cell proliferation. ..
  21. pmc Adipose triglyceride lipase is implicated in fuel- and non-fuel-stimulated insulin secretion
    Marie Line Peyot
    Molecular Nutrition Unit and Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Montreal, Quebec H1W4A4, Canada
    J Biol Chem 284:16848-59. 2009
    ..The results demonstrate the importance of ATGL and intracellular lipid signaling for fuel- and non-fuel-induced insulin secretion...
  22. doi request reprint Early detection of liver steatosis by magnetic resonance imaging in rats infused with glucose and intralipid solutions and correlation to insulin levels
    Gaspard d'Assignies
    Department of Radiology, University of Montreal, Hopital Saint Luc, 1058 rue Saint Denis, Montreal, Quebec, Canada, H2X 3J4 Research Center CHUM, Hopital Saint Luc, 264, René Lévesque Blvd East, Montreal, Quebec, Canada, H2X 1P1 Department of Radiology, Beaujon Hospital, Universite Paris VII, 100 bd du Général Leclerc, 92118 Clichy, France
    Metabolism 62:1850-7. 2013
    ....
  23. pmc GPR40 is necessary but not sufficient for fatty acid stimulation of insulin secretion in vivo
    Martin G Latour
    Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, H1W 4A4 Canada
    Diabetes 56:1087-94. 2007
    ..We conclude that GPR40 contributes approximately half of the full acute insulin secretory response to fatty acids in mice but does not play a role in the mechanisms by which fatty acids chronically impair insulin secretion...
  24. doi request reprint Fatty acid receptor Gpr40 mediates neuromicrovascular degeneration induced by transarachidonic acids in rodents
    Jean Claude Honoré
    Department of Pediatrics, Research Center CHU Ste Justine, Montreal, Quebec, Canada
    Arterioscler Thromb Vasc Biol 33:954-61. 2013
    ..We hypothesized that TAAs play a significant role in neonatal HI-induced cerebral microvascular degeneration through GPR40 activation...
  25. pmc G protein-coupled receptors and insulin secretion: 119 and counting
    S R Murthy Madiraju
    Montreal Diabetes Research Centre, University of Montreal, 2901 Rachel East, Montreal, Quebec, Canada
    Endocrinology 148:2598-600. 2007
  26. doi request reprint [Membrane fatty acid receptors in the β-cell: novel therapeutic targets for type 2 diabetes]
    Arturo D Mancini
    département de médecine de l université de Montréal, Montréal H1W 4A4, Quebec, Canada
    Med Sci (Paris) 29:715-21. 2013
    ..However, our understanding of the biology and pharmacology of these receptors remains incomplete. ..
  27. ncbi request reprint A role for the malonyl-CoA/long-chain acyl-CoA pathway of lipid signaling in the regulation of insulin secretion in response to both fuel and nonfuel stimuli
    Raphael Roduit
    Molecular Nutrition Unit, Department of Nutrition, University of Montréal and the Centre Hospitalier de l Université de Montréal, Montreal, Quebec, Canada
    Diabetes 53:1007-19. 2004
    ..The data show the existence of important links between the metabolic coupling factor malonyl-CoA, the partitioning of fatty acids, and the stimulation of insulin secretion to both fuel and nonfuel stimuli...
  28. doi request reprint The fatty acid receptor FFA1/GPR40 a decade later: how much do we know?
    Arturo D Mancini
    Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Department of Medicine, Universite de Montreal, Montreal, QC, Canada
    Trends Endocrinol Metab 24:398-407. 2013
    ..Here we summarize the current state of knowledge and emerging concepts regarding the role of GPR40 in regulating glucose homeostasis. ..
  29. ncbi request reprint Increasing triglyceride synthesis inhibits glucose-induced insulin secretion in isolated rat islets of langerhans: a study using adenoviral expression of diacylglycerol acyltransferase
    Cynthia L Kelpe
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    Endocrinology 143:3326-32. 2002
    ..We conclude that a selective increase in TG content impairs glucose-induced insulin secretion, a mechanism likely to mediate, at least in part, the deleterious effects of FA on pancreatic beta-cell function...
  30. ncbi request reprint The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription
    Li Zhao
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 280:11887-94. 2005
    ..Collectively, these results suggest that MafA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells...
  31. ncbi request reprint Pancreatic islet response to hyperglycemia is dependent on peroxisome proliferator-activated receptor alpha (PPARalpha)
    Hélène Bihan
    INSERM U 341, Hotel Dieu, Paris, France
    FEBS Lett 579:2284-8. 2005
    ..As such, islet PPARalpha could contribute to delay the progression of type 2 diabetes...
  32. pmc Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans
    Derek K Hagman
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    J Biol Chem 280:32413-8. 2005
    ..These results demonstrate that prolonged exposure of islets to palmitate inhibits insulin gene transcription by impairing nuclear localization of PDX-1 and cellular expression of MafA...
  33. ncbi request reprint Insulin secretory deficiency and glucose intolerance in Rab3A null mice
    Kazuro Yaekura
    Pacific Northwest Research Institute and Departments of Pharmacology and Medicine, University of Washington, Seattle, Washington 98122, USA
    J Biol Chem 278:9715-21. 2003
    ..Also, this study indicates, for the first time, that the in vivo insulin secretory dysfunction found in type-2 diabetes can lie solely at the level of defective insulin exocytosis...
  34. ncbi request reprint The ins and outs of fatty acids on the pancreatic beta cell
    Vincent Poitout
    Pacific Northwest Research Institute and Department of Medicine, University of Washington, Seattle, WA, USA
    Trends Endocrinol Metab 14:201-3. 2003
  35. ncbi request reprint Elevated glucose attenuates human insulin gene promoter activity in INS-1 pancreatic beta-cells via reduced nuclear factor binding to the A5/core and Z element
    Maria F Pino
    Department of Physiology, Michigan State University, 3176 Biomedical and Physical Sciences Building, East Lansing, MI 48824, USA
    Mol Endocrinol 19:1343-60. 2005
    ....
  36. ncbi request reprint Palmitate inhibition of insulin gene expression is mediated at the transcriptional level via ceramide synthesis
    Cynthia L Kelpe
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    J Biol Chem 278:30015-21. 2003
    ..We conclude that prolonged exposure to elevated levels of palmitate affects glucose-stimulated insulin gene expression via transcriptional mechanisms and ceramide synthesis...
  37. ncbi request reprint Beta-cell lipotoxicity: burning fat into heat?
    Vincent Poitout
    Endocrinology 145:3563-5. 2004
  38. ncbi request reprint Differential effects of hyperlipidemia on insulin secretion in islets of langerhans from hyperglycemic versus normoglycemic rats
    Isabelle Briaud
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    Diabetes 51:662-8. 2002
    ..We conclude that hyperlipidemia induced by high-fat feeding affects insulin secretion in islets from hyperglycemic GK rats only, by a mechanism which may involve, at least in part, modulation of UCP-2 expression...
  39. ncbi request reprint Minireview: Secondary beta-cell failure in type 2 diabetes--a convergence of glucotoxicity and lipotoxicity
    Vincent Poitout
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    Endocrinology 143:339-42. 2002
    ..We propose that chronic hyperglycemia, independent of hyperlipidemia, is toxic for beta-cell function, whereas chronic hyperlipidemia is deleterious only in the context of concomitant hyperglycemia...
  40. ncbi request reprint Evidence against the involvement of oxidative stress in fatty acid inhibition of insulin secretion
    Patrick C Moore
    Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA
    Diabetes 53:2610-6. 2004
    ....
  41. ncbi request reprint Effect of the two-layer (University of Wisconsin solution-perfluorochemical plus O2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton Isolation Protocol
    Shinichi Matsumoto
    Islet and Cell Processing Laboratory ICPL at Puget Sound Blood Center and Northwest Tissue Center, Seattle, WA, USA
    Transplantation 74:1414-9. 2002
    ..In this study, we hypothesized that the two-layer method (TLM) could extend the acceptable preservation period of pancreata before islet isolation and increase islet yields...
  42. ncbi request reprint Beta-cell glucose toxicity, lipotoxicity, and chronic oxidative stress in type 2 diabetes
    R Paul Robertson
    Pacific Northwest Research Institute and the Departments of Medicine and Pharmacology, University of Washington, Seattle, Washington 98122, USA
    Diabetes 53:S119-24. 2004
    ....

Research Grants10

  1. Mechanisms of Fatty-Acid Inhibition of the Insulin Gene
    Vincent Poitout; Fiscal Year: 2009
    ..It will provide new therapeutic targets aimed at preserving insulin secretion in type 2 diabetes, a devastating disease that affects more than 18 million Americans. ..
  2. Mechanisms of Fatty-Acid Inhibition of the Insulin Gene
    Vincent Poitout; Fiscal Year: 2007
    ..It will provide new therapeutic targets aimed at preserving insulin secretion in type 2 diabetes, a devastating disease that affects more than 18 million Americans. ..
  3. Role of GPR40 in the regulation of insulin secretion
    Vincent Poitout; Fiscal Year: 2005
    ..As an alternative approach, we will knock down GPR40 expression in isolated adult mouse islets by RNA silencing using adenoviral technology. ..
  4. Neutral lipid dysregulation of the pancreatic beta-cell
    Vincent Poitout; Fiscal Year: 2004
    ..These experiments will provide important insights into the pathophysiology of beta-cell dysfunction of type 2 diabetes, and have clear implications for the treatment of this disease. ..
  5. Mechanisms of Fatty-Acid Inhibition of the Insulin Gene
    Vincent Poitout; Fiscal Year: 2010
    ..It will provide new therapeutic targets aimed at preserving insulin secretion in type 2 diabetes, a devastating disease that affects more than 18 million Americans. ..