Jason J LeBlanc

Summary

Affiliation: Nova Scotia
Country: Canada

Publications

  1. pmc A cost effective real-time PCR for the detection of adenovirus from viral swabs
    Turkiya Al-Siyabi
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Room 404B, Mackenzie Building, 5788 University Ave, Halifax NS B3H 1V8, Canada
    Virol J 10:184. 2013
  2. doi request reprint Homogenization with heat treatment: A cost effective alternative to nucleic acid extraction for herpes simplex virus real-time PCR from viral swabs
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 179:261-4. 2012
  3. pmc Switching gears for an influenza pandemic: validation of a duplex reverse transcriptase PCR assay for simultaneous detection and confirmatory identification of pandemic (H1N1) 2009 influenza virus
    Jason J LeBlanc
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, 5788 University Avenue, Room 315, Mackenzie Building, Halifax, Nova Scotia B3H 1V8, Canada
    J Clin Microbiol 47:3805-13. 2009
  4. pmc Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:2786-9. 2008
  5. pmc Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:4049-51. 2008
  6. doi request reprint Difficulty with mumps diagnosis: what is the contribution of mumps mimickers?
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Nova Scotia, Canada
    J Clin Virol 46:381-3. 2009
  7. doi request reprint Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 151:158-60. 2008

Collaborators

Detail Information

Publications7

  1. pmc A cost effective real-time PCR for the detection of adenovirus from viral swabs
    Turkiya Al-Siyabi
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Room 404B, Mackenzie Building, 5788 University Ave, Halifax NS B3H 1V8, Canada
    Virol J 10:184. 2013
    ..2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture...
  2. doi request reprint Homogenization with heat treatment: A cost effective alternative to nucleic acid extraction for herpes simplex virus real-time PCR from viral swabs
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 179:261-4. 2012
    ..With a cost of less than $1 USD per extraction, this method provides an economical, rapid, and effective method to recover HSV-1/2 DNA from swabs suitable for real-time HSV PCR...
  3. pmc Switching gears for an influenza pandemic: validation of a duplex reverse transcriptase PCR assay for simultaneous detection and confirmatory identification of pandemic (H1N1) 2009 influenza virus
    Jason J LeBlanc
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, 5788 University Avenue, Room 315, Mackenzie Building, Halifax, Nova Scotia B3H 1V8, Canada
    J Clin Microbiol 47:3805-13. 2009
    ....
  4. pmc Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:2786-9. 2008
    ..HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study...
  5. pmc Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:4049-51. 2008
    ..LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks...
  6. doi request reprint Difficulty with mumps diagnosis: what is the contribution of mumps mimickers?
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Nova Scotia, Canada
    J Clin Virol 46:381-3. 2009
    ..In February 2007, mumps re-emerged in university students in Nova Scotia. Despite highly sensitive methods for mumps virus detection, only 14% (298/2082) of cases during the peak of the outbreak were laboratory confirmed...
  7. doi request reprint Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 151:158-60. 2008
    ..Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added...