Jason J LeBlanc

Summary

Affiliation: Nova Scotia
Country: Canada

Publications

  1. ncbi request reprint Detection of circulating norovirus genotypes: hitting a moving target
    Brenda Lee Rooney
    Dalhousie University, Halifax, Nova Scotia, Canada
    Virol J 11:129. 2014
  2. doi request reprint Homogenization with heat treatment: A cost effective alternative to nucleic acid extraction for herpes simplex virus real-time PCR from viral swabs
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 179:261-4. 2012
  3. pmc Switching gears for an influenza pandemic: validation of a duplex reverse transcriptase PCR assay for simultaneous detection and confirmatory identification of pandemic (H1N1) 2009 influenza virus
    Jason J LeBlanc
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, 5788 University Avenue, Room 315, Mackenzie Building, Halifax, Nova Scotia B3H 1V8, Canada
    J Clin Microbiol 47:3805-13. 2009
  4. pmc Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:2786-9. 2008
  5. pmc Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:4049-51. 2008
  6. pmc Detection of influenza H7N9 virus: all molecular tests are not equal
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority CDHA, Halifax, Nova Scotia, Canada
    J Clin Microbiol 51:3835-8. 2013
  7. doi request reprint Difficulty with mumps diagnosis: what is the contribution of mumps mimickers?
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Nova Scotia, Canada
    J Clin Virol 46:381-3. 2009
  8. ncbi request reprint Molecular detection of varicella zoster virus while keeping an eye on the budget
    Khalifa Binkhamis
    Department of Pathology and Laboratory Medicine, Capital District Health Authority, Halifax, Nova Scotia, Canada Dalhousie University, Halifax, Nova Scotia, Canada
    J Virol Methods 202:24-7. 2014
  9. pmc A cost effective real-time PCR for the detection of adenovirus from viral swabs
    Turkiya Al-Siyabi
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Room 404B, Mackenzie Building, 5788 University Ave, Halifax NS B3H 1V8, Canada
    Virol J 10:184. 2013
  10. ncbi request reprint Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 151:158-60. 2008

Collaborators

Detail Information

Publications11

  1. ncbi request reprint Detection of circulating norovirus genotypes: hitting a moving target
    Brenda Lee Rooney
    Dalhousie University, Halifax, Nova Scotia, Canada
    Virol J 11:129. 2014
    ..This study evaluated the analytical and clinical performance characteristics of one real-time RT-PCR and two end-point RT-PCRs commonly used in microbiology laboratories...
  2. doi request reprint Homogenization with heat treatment: A cost effective alternative to nucleic acid extraction for herpes simplex virus real-time PCR from viral swabs
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 179:261-4. 2012
    ..With a cost of less than $1 USD per extraction, this method provides an economical, rapid, and effective method to recover HSV-1/2 DNA from swabs suitable for real-time HSV PCR...
  3. pmc Switching gears for an influenza pandemic: validation of a duplex reverse transcriptase PCR assay for simultaneous detection and confirmatory identification of pandemic (H1N1) 2009 influenza virus
    Jason J LeBlanc
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, 5788 University Avenue, Room 315, Mackenzie Building, Halifax, Nova Scotia B3H 1V8, Canada
    J Clin Microbiol 47:3805-13. 2009
    ....
  4. pmc Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:2786-9. 2008
    ..HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study...
  5. pmc Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada
    J Clin Microbiol 46:4049-51. 2008
    ..LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks...
  6. pmc Detection of influenza H7N9 virus: all molecular tests are not equal
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority CDHA, Halifax, Nova Scotia, Canada
    J Clin Microbiol 51:3835-8. 2013
    ....
  7. doi request reprint Difficulty with mumps diagnosis: what is the contribution of mumps mimickers?
    Todd F Hatchette
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Nova Scotia, Canada
    J Clin Virol 46:381-3. 2009
    ..In February 2007, mumps re-emerged in university students in Nova Scotia. Despite highly sensitive methods for mumps virus detection, only 14% (298/2082) of cases during the peak of the outbreak were laboratory confirmed...
  8. ncbi request reprint Molecular detection of varicella zoster virus while keeping an eye on the budget
    Khalifa Binkhamis
    Department of Pathology and Laboratory Medicine, Capital District Health Authority, Halifax, Nova Scotia, Canada Dalhousie University, Halifax, Nova Scotia, Canada
    J Virol Methods 202:24-7. 2014
    ..8%. Overall, homogenization and heat treatment combined with a qualitative in-house real-time PCR is a rapid, accurate and cost effective method for the detection of VZV. ..
  9. pmc A cost effective real-time PCR for the detection of adenovirus from viral swabs
    Turkiya Al-Siyabi
    Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority, Room 404B, Mackenzie Building, 5788 University Ave, Halifax NS B3H 1V8, Canada
    Virol J 10:184. 2013
    ..2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture...
  10. ncbi request reprint Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes
    Jason J LeBlanc
    Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada
    J Virol Methods 151:158-60. 2008
    ..Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added...
  11. pmc Detection and differentiation of herpes simplex viruses by use of the viper platform: advantages, limitations, and concerns
    Amanda L S Lang
    Dalhousie University, Halifax, Nova Scotia, Canada
    J Clin Microbiol 52:2186-8. 2014
    ..The HSV-Q(x) assay was found to be highly sensitive and accurate; however, a gray zone may be required for specimens with values falling between 50 and 800 maximum relative fluorescence units. ..