Robert G Rutledge

Summary

Affiliation: Natural Resources Canada
Country: Canada

Publications

  1. pmc Potential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca)
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, Quebec, QC G1V 4C7, Canada
    BMC Plant Biol 13:116. 2013
  2. pmc A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, 1055 du PEPS, Quebec, Quebec G1V 4C7, Canada
    BMC Biotechnol 8:47. 2008
  3. pmc Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, 1055 du PEPS, Quebec, Quebec G1V 4C7, Canada
    BMC Mol Biol 9:96. 2008
  4. pmc Assessing the performance capabilities of LRE-based assays for absolute quantitative real-time PCR
    Robert G Rutledge
    Canadian Forest Service, Natural Resources Canada, Quebec, Quebec, Canada
    PLoS ONE 5:e9731. 2010
  5. pmc A Java program for LRE-based real-time qPCR that enables large-scale absolute quantification
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, Quebec, Canada
    PLoS ONE 6:e17636. 2011
  6. doi request reprint Initiation of somatic embryos and regeneration of plants from primordial shoots of 10-year-old somatic white spruce and expression profiles of 11 genes followed during the tissue culture process
    Krystyna Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, P O Box 10380, Stn Sainte Foy, Quebec, QC, G1V 4C7, Canada
    Planta 233:635-47. 2011
  7. ncbi request reprint Characterization and tissue-specific expression of two lepidopteran farnesyl diphosphate synthase homologs: implications for the biosynthesis of ethyl-substituted juvenile hormones
    Michel Cusson
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Quebec City, Quebec G1V 4C7, Canada
    Proteins 65:742-58. 2006
  8. doi request reprint Hormonally regulated overexpression of Arabidopsis WUS and conifer LEC1 (CHAP3A) in transgenic white spruce: implications for somatic embryo development and somatic seedling growth
    Krystyna Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Quebec, QC, Canada
    Plant Cell Rep 29:723-34. 2010

Detail Information

Publications8

  1. pmc Potential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca)
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, Quebec, QC G1V 4C7, Canada
    BMC Plant Biol 13:116. 2013
    ....
  2. pmc A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, 1055 du PEPS, Quebec, Quebec G1V 4C7, Canada
    BMC Biotechnol 8:47. 2008
    ....
  3. pmc Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, 1055 du PEPS, Quebec, Quebec G1V 4C7, Canada
    BMC Mol Biol 9:96. 2008
    ..Called "linear regression of efficiency" or LRE, this kinetic-based approach redefines amplification efficiency as the maximal efficiency (Emax) generated at the onset of thermocycling...
  4. pmc Assessing the performance capabilities of LRE-based assays for absolute quantitative real-time PCR
    Robert G Rutledge
    Canadian Forest Service, Natural Resources Canada, Quebec, Quebec, Canada
    PLoS ONE 5:e9731. 2010
    ....
  5. pmc A Java program for LRE-based real-time qPCR that enables large-scale absolute quantification
    Robert G Rutledge
    Natural Resources Canada, Canadian Forest Service, Quebec, Canada
    PLoS ONE 6:e17636. 2011
    ..However, a major challenge of implementing LRE quantification is the labor intensive nature of the analysis...
  6. doi request reprint Initiation of somatic embryos and regeneration of plants from primordial shoots of 10-year-old somatic white spruce and expression profiles of 11 genes followed during the tissue culture process
    Krystyna Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, P O Box 10380, Stn Sainte Foy, Quebec, QC, G1V 4C7, Canada
    Planta 233:635-47. 2011
    ..Mature somatic embryos and plants were produced from the explants of responding genotype. Implication of these results for future research on adult conifer recalcitrance in micropropagation is discussed...
  7. ncbi request reprint Characterization and tissue-specific expression of two lepidopteran farnesyl diphosphate synthase homologs: implications for the biosynthesis of ethyl-substituted juvenile hormones
    Michel Cusson
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Quebec City, Quebec G1V 4C7, Canada
    Proteins 65:742-58. 2006
    ..These results suggest that type-II FPPS plays a leading role in lepidopteran JH biosynthesis in spite of its apparently more conventional catalytic cavity...
  8. doi request reprint Hormonally regulated overexpression of Arabidopsis WUS and conifer LEC1 (CHAP3A) in transgenic white spruce: implications for somatic embryo development and somatic seedling growth
    Krystyna Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Quebec, QC, Canada
    Plant Cell Rep 29:723-34. 2010
    ..Implications of these results for further work are discussed...