S E Douglas

Summary

Affiliation: National Research Council
Country: Canada

Publications

  1. ncbi request reprint Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
    Susan E Douglas
    Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
    Dev Comp Immunol 27:589-601. 2003
  2. ncbi request reprint Structural characterization of the antimicrobial peptide pleurocidin from winter flounder
    Raymond T Syvitski
    College of Pharmacy, 5968 College Street, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5
    Biochemistry 44:7282-93. 2005
  3. ncbi request reprint Exploring the diversity of marine-derived fungal polyketide synthases
    Kimberly M Mayer
    Center for Marine Science, University of North Carolina at Wilmington, 5600 Marvin Moss Lane, Wilmington, NC 28409, USA
    Can J Microbiol 53:291-302. 2007
  4. ncbi request reprint Identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum)
    Susan E Douglas
    Institute for Marine Biosciences, Halifax, Nova Scotia, Canada
    Eur J Biochem 270:3720-30. 2003
  5. ncbi request reprint Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum)
    S E Douglas
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, B3H 3Z1, Nova Scotia, Canada
    Dev Comp Immunol 25:137-47. 2001
  6. ncbi request reprint The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae
    S E Douglas
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
    J Mol Evol 48:236-44. 1999
  7. ncbi request reprint Plastid evolution: origins, diversity, trends
    S E Douglas
    Canadian Institute for Advanced Research, Program in Evolutionary Biology, National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada
    Curr Opin Genet Dev 8:655-61. 1998
  8. ncbi request reprint Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
    A L Greenshields
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
    Protein Pept Lett 15:985-94. 2008
  9. ncbi request reprint The small subunit of ribulose-1,5-bisphosphate carboxylase is plastid-encoded in the chlorophyll c-containing alga Cryptomonas phi
    S E Douglas
    Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada
    Plant Mol Biol 13:13-20. 1989
  10. ncbi request reprint Identification of immune-relevant genes from atlantic salmon using suppression subtractive hybridization
    S C M Tsoi
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada
    Mar Biotechnol (NY) 6:199-214. 2004

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
    Susan E Douglas
    Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
    Dev Comp Immunol 27:589-601. 2003
    ..Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish...
  2. ncbi request reprint Structural characterization of the antimicrobial peptide pleurocidin from winter flounder
    Raymond T Syvitski
    College of Pharmacy, 5968 College Street, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5
    Biochemistry 44:7282-93. 2005
    ..From diffusion studies it was determined that each micelle contains at least two pleurocidin molecules...
  3. ncbi request reprint Exploring the diversity of marine-derived fungal polyketide synthases
    Kimberly M Mayer
    Center for Marine Science, University of North Carolina at Wilmington, 5600 Marvin Moss Lane, Wilmington, NC 28409, USA
    Can J Microbiol 53:291-302. 2007
    ..Evaluating primer-binding sites was necessary to obtain KS domain fragments from putative PKSs while maintaining a level of sequence information adequate to properly classify and characterize them...
  4. ncbi request reprint Identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum)
    Susan E Douglas
    Institute for Marine Biosciences, Halifax, Nova Scotia, Canada
    Eur J Biochem 270:3720-30. 2003
    ....
  5. ncbi request reprint Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum)
    S E Douglas
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, B3H 3Z1, Nova Scotia, Canada
    Dev Comp Immunol 25:137-47. 2001
    ....
  6. ncbi request reprint The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae
    S E Douglas
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
    J Mol Evol 48:236-44. 1999
    ..Furthermore, recombination events involving both tRNA genes and the rRNA cistrons appear to have been responsible for the structure of the cryptophyte plastid genome, including the formation of the inverted repeat...
  7. ncbi request reprint Plastid evolution: origins, diversity, trends
    S E Douglas
    Canadian Institute for Advanced Research, Program in Evolutionary Biology, National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada
    Curr Opin Genet Dev 8:655-61. 1998
    ....
  8. ncbi request reprint Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
    A L Greenshields
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
    Protein Pept Lett 15:985-94. 2008
    ..Expression was attempted in baculovirus and bacterial expression systems and the various purification and refolding methods used to determine the optimal strategy for production of active, correctly refolded hepcidin are reviewed...
  9. ncbi request reprint The small subunit of ribulose-1,5-bisphosphate carboxylase is plastid-encoded in the chlorophyll c-containing alga Cryptomonas phi
    S E Douglas
    Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada
    Plant Mol Biol 13:13-20. 1989
    ....
  10. ncbi request reprint Identification of immune-relevant genes from atlantic salmon using suppression subtractive hybridization
    S C M Tsoi
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada
    Mar Biotechnol (NY) 6:199-214. 2004
    ....
  11. doi request reprint Expression, purification and structural characterization of recombinant hepcidin, an antimicrobial peptide identified in Japanese flounder, Paralichthys olivaceus
    B Srinivasulu
    Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, Canada
    Protein Expr Purif 61:36-44. 2008
    ..NMR-based studies showed that hepcidin contained a rare vicinal disulfide linkage at the top of a loop structure and a short beta-sheet structure encompassing residues 7-13 and 19-25 that is stabilized by three disulfide bonds...
  12. ncbi request reprint Trypsinogen expression during the development of the exocrine pancreas in winter flounder (Pleuronectes americanus)
    H M Murray
    AquaNet, Memorial University of Newfoundland, St John s, Canada
    Comp Biochem Physiol A Mol Integr Physiol 138:53-9. 2004
    ..The tissue expression patterns observed here are likely due in part to the diffuse nature of the pancreas. Trypsin-like activity was evident from hatch and continued at significant levels through to at least 25 dph...
  13. doi request reprint Translational machinery of senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.): comparative sequence analysis of the complete set of 60s ribosomal proteins and their expression
    Makoto P Matsuoka
    Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, Halifax, Nova Scotia, B3H 3Z1, Canada
    Mar Biotechnol (NY) 10:676-91. 2008
    ..The genomic resources and knowledge developed in this survey will be useful for the study of Pleuronectiformes evolution...
  14. doi request reprint Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus L
    Anthony J Manning
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, B3H 3Z1 Canada
    J Endocrinol 196:181-92. 2008
    ..Patterns of preproghrelin expression suggest that ghrelin has important roles during and after larval development in halibut, and that ghrelin is associated with digestive and gonadal tissues in this teleost...
  15. ncbi request reprint Cellular localization of pleurocidin gene expression and synthesis in winter flounder gill using immunohistochemistry and in situ hybridization
    Harry M Murray
    AquaNet, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
    Cell Tissue Res 312:197-202. 2003
    ..These observations suggest that pleurocidin is expressed in not one but two distinct populations of cells within the winter flounder, one being an important group of inflammatory cells, the eosinophilic granular cells...
  16. pmc Nucleotide sequence of the genes for ribosomal protein S4 and tRNA(Arg) from the chlorophyll c-containing alga Cryptomonas phi
    S E Douglas
    Atlantic Research Laboratory, National Research Council of Canada, Halifax, Nova Scotia
    Nucleic Acids Res 18:1903. 1990
  17. ncbi request reprint Unusual organization of a ribosomal protein operon in the plastid genome of Cryptomonas phi: evolutionary considerations
    S E Douglas
    Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada
    Curr Genet 19:289-94. 1991
    ..Transcript analysis suggests that these genes are co-transcribed along with several up and/or down-stream genes. The evolutionary significance of this unique gene organization is discussed...
  18. doi request reprint Effect of early introduction of microencapsulated diet to larval Atlantic halibut, Hippoglossus hippoglossus L. assessed by microarray analysis
    H M Murray
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada, B3H 3Z1
    Mar Biotechnol (NY) 12:214-29. 2010
    ..Interestingly, several of these genes were involved in pigmentation and eye development, in agreement with the phenotypic abnormalities seen in the larvae...
  19. ncbi request reprint Identification of genes differentially expressed in Atlantic salmon (Salmo salar) in response to infection by Aeromonas salmonicida using cDNA microarray technology
    K Vanya Ewart
    Institute for Marine Biosciences, Halifax, NS, Canada B3H 3Z1
    Dev Comp Immunol 29:333-47. 2005
    ..In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity...
  20. pmc Novel antimicrobial peptides derived from flatfish genes
    Aleksander Patrzykat
    Institute for Marine Biosciences, National Research Council of Canada, Halifax NS B3H 3Z1, Canada
    Antimicrob Agents Chemother 47:2464-70. 2003
    ..albicans. The methods described here will be useful for the identification of novel peptides with good antimicrobial activities...
  21. ncbi request reprint A secY homologue is found in the plastid genome of Cryptomonas phi
    S E Douglas
    Institute for Marine Biosciences, National Research Council, Halifax, Nova Scotia, Canada
    FEBS Lett 298:93-6. 1992
    ..coli homologue. This report of a secY homologue in a plastid genome provides preliminary evidence that a prokaryotic-like protein export system may be operating in plastids...
  22. ncbi request reprint Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in Atlantic salmon during Aeromonas salmonicida infection
    Stephen Tsoi
    National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, NS, Canada
    Vet Immunol Immunopathol 109:183-7. 2006
    ....
  23. pmc Antimicrobial activities of jadomycin B and structurally related analogues
    David L Jakeman
    College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
    Antimicrob Agents Chemother 53:1245-7. 2009
    ..The most active of these jadomycin analogues showed good activity against a variety of staphylococci, including methicillin-resistant Staphylococcus aureus...
  24. pmc Comprehensive EST analysis of Atlantic halibut (Hippoglossus hippoglossus), a commercially relevant aquaculture species
    Susan E Douglas
    Institute for Marine Biosciences, Halifax, Nova Scotia, Canada
    BMC Genomics 8:144. 2007
    ..This forms the basis for subsequent microarray design, SNP detection and the placement of novel markers on genetic linkage maps...
  25. ncbi request reprint A C-terminal glycine suppresses production of pleurocidin as a fusion peptide in Escherichia coli
    Brian C Bryksa
    NRC Institute for Marine Biosciences, National Research Council Canada, Halifax, NS, Canada B3H 3Z1
    Protein Expr Purif 45:88-98. 2006
    ..This antimicrobial effect is probably facilitated by residual solubility of the fusion peptide and by a C-terminal cap structure, which stabilizes the r-pleurocidin-G alpha-helix that is thought to be important for activity...
  26. ncbi request reprint The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish
    K Vanya Ewart
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
    Dev Comp Immunol 32:380-90. 2008
    ..Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response...
  27. ncbi request reprint Antimicrobial peptides: cooperative approaches to protection
    Aleksander Patrzykat
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada, B3H 3Z1
    Protein Pept Lett 12:19-25. 2005
    ....
  28. ncbi request reprint Gone gene fishing: how to catch novel marine antimicrobials
    Aleksander Patrzykat
    Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
    Trends Biotechnol 21:362-9. 2003
    ..The past three years has seen a revolution in the methods used to identify novel antimicrobials from marine sources; among the most promising are marine cationic antimicrobial peptides (CAPs)...
  29. ncbi request reprint Isolation and characterization of cDNA sequences of L-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbic acid, from extant primitive fish groups
    Young Sun Cho
    Department of Aquaculture, Pukyong National University, Busan 608 737, South Korea
    Comp Biochem Physiol B Biochem Mol Biol 147:178-90. 2007
    ..The transcription and enzyme activity of GULO were kidney-specific when measured by biochemical assay and reverse transcription-PCR...
  30. pmc Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): phylogeny and tissue- and development-specific
    Manuel Manchado
    IFAPA Centro El Toruño, Junta de Andalucía Camino Tiro de pichón s n, El Puerto de Santa María, Cadiz, Spain
    BMC Evol Biol 7:107. 2007
    ..Their high representation in cDNA libraries has resulted in the increase of RP sequences available from different organisms and their proposal as appropriate molecular markers for phylogenetic analysis...
  31. ncbi request reprint Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
    Ann Elise O Jordal
    National Institute of Nutrition and Seafood Research, N 5817 Bergen, Norway
    J Nutr 135:2355-61. 2005
    ..This partially validated microarray may be used for further gene expression profiling using other dietary comparisons, and for further characterization of selected genes...