Research Topics
| S E DouglasSummaryAffiliation: National Research Council Country: Canada Publications
| Collaborators
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Detail Information
Publications
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fishSusan E Douglas
Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
Dev Comp Immunol 27:589-601. 2003..Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish...- Structural characterization of the antimicrobial peptide pleurocidin from winter flounderRaymond T Syvitski
College of Pharmacy, 5968 College Street, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5
Biochemistry 44:7282-93. 2005..From diffusion studies it was determined that each micelle contains at least two pleurocidin molecules... - Exploring the diversity of marine-derived fungal polyketide synthasesKimberly M Mayer
Center for Marine Science, University of North Carolina at Wilmington, 5600 Marvin Moss Lane, Wilmington, NC 28409, USA
Can J Microbiol 53:291-302. 2007..Evaluating primer-binding sites was necessary to obtain KS domain fragments from putative PKSs while maintaining a level of sequence information adequate to properly classify and characterize them... - Identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum)Susan E Douglas
Institute for Marine Biosciences, Halifax, Nova Scotia, Canada
Eur J Biochem 270:3720-30. 2003.... - Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum)S E Douglas
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, B3H 3Z1, Nova Scotia, Canada
Dev Comp Immunol 25:137-47. 2001.... - The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algaeS E Douglas
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
J Mol Evol 48:236-44. 1999..Furthermore, recombination events involving both tRNA genes and the rRNA cistrons appear to have been responsible for the structure of the cryptophyte plastid genome, including the formation of the inverted repeat... - Plastid evolution: origins, diversity, trendsS E Douglas
Canadian Institute for Advanced Research, Program in Evolutionary Biology, National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada
Curr Opin Genet Dev 8:655-61. 1998.... - Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptidesA L Greenshields
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
Protein Pept Lett 15:985-94. 2008..Expression was attempted in baculovirus and bacterial expression systems and the various purification and refolding methods used to determine the optimal strategy for production of active, correctly refolded hepcidin are reviewed... - The small subunit of ribulose-1,5-bisphosphate carboxylase is plastid-encoded in the chlorophyll c-containing alga Cryptomonas phiS E Douglas
Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada
Plant Mol Biol 13:13-20. 1989.... - Identification of immune-relevant genes from atlantic salmon using suppression subtractive hybridizationS C M Tsoi
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada
Mar Biotechnol (NY) 6:199-214. 2004.... 
Expression, purification and structural characterization of recombinant hepcidin, an antimicrobial peptide identified in Japanese flounder, Paralichthys olivaceusB Srinivasulu
Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, Canada
Protein Expr Purif 61:36-44. 2008..NMR-based studies showed that hepcidin contained a rare vicinal disulfide linkage at the top of a loop structure and a short beta-sheet structure encompassing residues 7-13 and 19-25 that is stabilized by three disulfide bonds...- Trypsinogen expression during the development of the exocrine pancreas in winter flounder (Pleuronectes americanus)H M Murray
AquaNet, Memorial University of Newfoundland, St John s, Canada
Comp Biochem Physiol A Mol Integr Physiol 138:53-9. 2004..The tissue expression patterns observed here are likely due in part to the diffuse nature of the pancreas. Trypsin-like activity was evident from hatch and continued at significant levels through to at least 25 dph... - Translational machinery of senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.): comparative sequence analysis of the complete set of 60s ribosomal proteins and their expressionMakoto P Matsuoka
Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, Halifax, Nova Scotia, B3H 3Z1, Canada
Mar Biotechnol (NY) 10:676-91. 2008..The genomic resources and knowledge developed in this survey will be useful for the study of Pleuronectiformes evolution... - Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus LAnthony J Manning
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, B3H 3Z1 Canada
J Endocrinol 196:181-92. 2008..Patterns of preproghrelin expression suggest that ghrelin has important roles during and after larval development in halibut, and that ghrelin is associated with digestive and gonadal tissues in this teleost... - Cellular localization of pleurocidin gene expression and synthesis in winter flounder gill using immunohistochemistry and in situ hybridizationHarry M Murray
AquaNet, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
Cell Tissue Res 312:197-202. 2003..These observations suggest that pleurocidin is expressed in not one but two distinct populations of cells within the winter flounder, one being an important group of inflammatory cells, the eosinophilic granular cells... 
Nucleotide sequence of the genes for ribosomal protein S4 and tRNA(Arg) from the chlorophyll c-containing alga Cryptomonas phiS E Douglas
Atlantic Research Laboratory, National Research Council of Canada, Halifax, Nova Scotia
Nucleic Acids Res 18:1903. 1990- Unusual organization of a ribosomal protein operon in the plastid genome of Cryptomonas phi: evolutionary considerationsS E Douglas
Atlantic Research Laboratory, National Research Council, Halifax, Nova Scotia, Canada
Curr Genet 19:289-94. 1991..Transcript analysis suggests that these genes are co-transcribed along with several up and/or down-stream genes. The evolutionary significance of this unique gene organization is discussed... - Effect of early introduction of microencapsulated diet to larval Atlantic halibut, Hippoglossus hippoglossus L. assessed by microarray analysisH M Murray
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada, B3H 3Z1
Mar Biotechnol (NY) 12:214-29. 2010..Interestingly, several of these genes were involved in pigmentation and eye development, in agreement with the phenotypic abnormalities seen in the larvae... - Identification of genes differentially expressed in Atlantic salmon (Salmo salar) in response to infection by Aeromonas salmonicida using cDNA microarray technologyK Vanya Ewart
Institute for Marine Biosciences, Halifax, NS, Canada B3H 3Z1
Dev Comp Immunol 29:333-47. 2005..In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity... - Novel antimicrobial peptides derived from flatfish genesAleksander Patrzykat
Institute for Marine Biosciences, National Research Council of Canada, Halifax NS B3H 3Z1, Canada
Antimicrob Agents Chemother 47:2464-70. 2003..albicans. The methods described here will be useful for the identification of novel peptides with good antimicrobial activities... - A secY homologue is found in the plastid genome of Cryptomonas phiS E Douglas
Institute for Marine Biosciences, National Research Council, Halifax, Nova Scotia, Canada
FEBS Lett 298:93-6. 1992..coli homologue. This report of a secY homologue in a plastid genome provides preliminary evidence that a prokaryotic-like protein export system may be operating in plastids... - Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in Atlantic salmon during Aeromonas salmonicida infectionStephen Tsoi
National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, NS, Canada
Vet Immunol Immunopathol 109:183-7. 2006.... - Antimicrobial activities of jadomycin B and structurally related analoguesDavid L Jakeman
College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
Antimicrob Agents Chemother 53:1245-7. 2009..The most active of these jadomycin analogues showed good activity against a variety of staphylococci, including methicillin-resistant Staphylococcus aureus... - Comprehensive EST analysis of Atlantic halibut (Hippoglossus hippoglossus), a commercially relevant aquaculture speciesSusan E Douglas
Institute for Marine Biosciences, Halifax, Nova Scotia, Canada
BMC Genomics 8:144. 2007..This forms the basis for subsequent microarray design, SNP detection and the placement of novel markers on genetic linkage maps... - A C-terminal glycine suppresses production of pleurocidin as a fusion peptide in Escherichia coliBrian C Bryksa
NRC Institute for Marine Biosciences, National Research Council Canada, Halifax, NS, Canada B3H 3Z1
Protein Expr Purif 45:88-98. 2006..This antimicrobial effect is probably facilitated by residual solubility of the fusion peptide and by a C-terminal cap structure, which stabilizes the r-pleurocidin-G alpha-helix that is thought to be important for activity... - The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fishK Vanya Ewart
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
Dev Comp Immunol 32:380-90. 2008..Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response... - Antimicrobial peptides: cooperative approaches to protectionAleksander Patrzykat
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada, B3H 3Z1
Protein Pept Lett 12:19-25. 2005.... - Gone gene fishing: how to catch novel marine antimicrobialsAleksander Patrzykat
Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1
Trends Biotechnol 21:362-9. 2003..The past three years has seen a revolution in the methods used to identify novel antimicrobials from marine sources; among the most promising are marine cationic antimicrobial peptides (CAPs)... - Isolation and characterization of cDNA sequences of L-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbic acid, from extant primitive fish groupsYoung Sun Cho
Department of Aquaculture, Pukyong National University, Busan 608 737, South Korea
Comp Biochem Physiol B Biochem Mol Biol 147:178-90. 2007..The transcription and enzyme activity of GULO were kidney-specific when measured by biochemical assay and reverse transcription-PCR... - Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): phylogeny and tissue- and development-specificManuel Manchado
IFAPA Centro El Toruño, Junta de Andalucía Camino Tiro de pichón s n, El Puerto de Santa María, Cadiz, Spain
BMC Evol Biol 7:107. 2007..Their high representation in cDNA libraries has resulted in the increase of RP sequences available from different organisms and their proposal as appropriate molecular markers for phylogenetic analysis... - Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)Ann Elise O Jordal
National Institute of Nutrition and Seafood Research, N 5817 Bergen, Norway
J Nutr 135:2355-61. 2005..This partially validated microarray may be used for further gene expression profiling using other dietary comparisons, and for further characterization of selected genes...