Gerard Wright

Summary

Affiliation: McMaster University
Country: Canada

Publications

  1. ncbi request reprint Domain-domain interactions in the aminoglycoside antibiotic resistance enzyme AAC(6')-APH(2'')
    David D Boehr
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5
    Biochemistry 43:9846-55. 2004
  2. ncbi request reprint Biochemistry. A new target for antibiotic development
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada, L8N 3Z5
    Science 315:1373-4. 2007
  3. doi request reprint Antibiotic resistance in the environment: a link to the clinic?
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada L8N 3Z5
    Curr Opin Microbiol 13:589-94. 2010
  4. doi request reprint The antibiotic resistome
    Gerard D Wright
    McMaster University, M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, Hamilton, ON, L8N 3Z5, Canada
    Expert Opin Drug Discov 5:779-88. 2010
  5. doi request reprint Antibiotic resistance is ancient: implications for drug discovery
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main St W, Hamilton, ON, L8S 4K1, Canada
    Trends Microbiol 20:157-9. 2012
  6. doi request reprint Antibiotics: a new hope
    Gerard D Wright
    MG DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Chem Biol 19:3-10. 2012
  7. doi request reprint Molecular mechanisms of antibiotic resistance
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main St W, Hamilton, ON, Canada
    Chem Commun (Camb) 47:4055-61. 2011
  8. ncbi request reprint Bacterial resistance to antibiotics: enzymatic degradation and modification
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada
    Adv Drug Deliv Rev 57:1451-70. 2005
  9. pmc Inactivation of the lipopeptide antibiotic daptomycin by hydrolytic mechanisms
    Vanessa M D'Costa
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry, and Biomedical Sciences, McMaster University, Hamilton, ON, Canada
    Antimicrob Agents Chemother 56:757-64. 2012
  10. doi request reprint Making sense of antisense in antibiotic drug discovery
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, ON, Canada
    Cell Host Microbe 6:197-8. 2009

Collaborators

  • Justin R Nodwell
  • David D Boehr
  • M A Valvano
  • Eric D Brown
  • Jeff Pootoolal
  • James McNulty
  • Allan Matte
  • Gaik Lean Chee
  • D B Northrop
  • Brian K Hubbard
  • James Kronstad
  • Christopher Walsh
  • M S Paget
  • Matthew I Hutchings
  • F Gao
  • Hee Jeon Hong
  • Donald W Hughes
  • Kalinka Koteva
  • Ishac Nazi
  • Patricia L Taylor
  • Tariq A Mukhtar
  • Sherry S Lamb
  • Kalinka P Koteva
  • Vanessa M D'Costa
  • Lindsay Kalan
  • Maulik Thaker
  • Kari Ann Draker
  • Rong Shi
  • Albert M Berghuis
  • David C Bareich
  • Julie Perry
  • Linda J Ejim
  • Nadine H Elowe
  • Linda Ejim
  • I Ahmad Mirza
  • Kun Zhang
  • Bijan Zakeri
  • Murray S Junop
  • Emma Griffiths
  • Gladys P De Leon
  • Magdalena Korczynska
  • Ian F Moore
  • Nancy L McKenzie
  • Tushar Shakya
  • Mariya Morar
  • Tejal Patel
  • Murray Junop
  • Miroslaw Cygler
  • Traian Sulea
  • Jennifer Baysarowich
  • David Bareich
  • Wangrong Yang
  • Xiaoming Li
  • Suzanne L Jacques
  • Nicole J Johnston
  • John M Neu
  • Paul R Thompson
  • Gianfranco de Pascale
  • Nicholas Waglechner
  • Seiji Sugiman-Marangos
  • Peter Spanogiannopoulos
  • Tom Kelly
  • Qizhi Cui
  • Sara Ebert
  • Kirandeep Bhullar
  • Ariane Proteau
  • John R Walker
  • Kim M Blakely
  • Fiona McArthur
  • Elena Evdokimova
  • Adam Scott
  • Jonathan D Chechetto
  • Jan E Blanchard
  • Peter R Williamson
  • Sathesh Bhat
  • Laura Rossi
  • Anita Sham
  • Rachael Sumerfield
  • Katherine M McGrann
  • J Concepcion Loredo-Osti
  • Danielle Malo
  • Steve Jenkins
  • Jonathan D Cechetto
  • Michela Zolli-Juran
  • Denis M Daigle
  • Christina Capone
  • Robert Kinach
  • Kirk Green
  • Theresa J Noga
  • Hoi Kiong Lai

Detail Information

Publications64

  1. ncbi request reprint Domain-domain interactions in the aminoglycoside antibiotic resistance enzyme AAC(6')-APH(2'')
    David D Boehr
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5
    Biochemistry 43:9846-55. 2004
    ..Domain-domain interactions in AAC(6')-APH(2' ') offer a unique target for inhibitor strategies, as we show that their disruption simultaneously inhibits both activities >90%...
  2. ncbi request reprint Biochemistry. A new target for antibiotic development
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada, L8N 3Z5
    Science 315:1373-4. 2007
  3. doi request reprint Antibiotic resistance in the environment: a link to the clinic?
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada L8N 3Z5
    Curr Opin Microbiol 13:589-94. 2010
    ..Understanding the extent of the environmental resistome and its mobilization into pathogenic bacteria is essential for the management and discovery of antibiotics...
  4. doi request reprint The antibiotic resistome
    Gerard D Wright
    McMaster University, M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, Hamilton, ON, L8N 3Z5, Canada
    Expert Opin Drug Discov 5:779-88. 2010
    ..There is, therefore, a great interest in understanding the origins, scope and evolution of antibiotic resistance...
  5. doi request reprint Antibiotic resistance is ancient: implications for drug discovery
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main St W, Hamilton, ON, L8S 4K1, Canada
    Trends Microbiol 20:157-9. 2012
    ..This prevalence of resistance, well before the use of antibiotics, denotes the importance of taking microbial chemical ecology and deep metagenomic profiling into account in the development and use of antibiotics...
  6. doi request reprint Antibiotics: a new hope
    Gerard D Wright
    MG DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Chem Biol 19:3-10. 2012
    ..This knowledge is fueling a renaissance of interest and innovation in antibiotic discovery, synthesis, and mechanism that is poised to inform drug discovery to address pressing clinical needs...
  7. doi request reprint Molecular mechanisms of antibiotic resistance
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main St W, Hamilton, ON, Canada
    Chem Commun (Camb) 47:4055-61. 2011
    ..Understanding the chemical rationale and underpinnings of resistance is an essential component of our response to this clinical challenge...
  8. ncbi request reprint Bacterial resistance to antibiotics: enzymatic degradation and modification
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada
    Adv Drug Deliv Rev 57:1451-70. 2005
    ....
  9. pmc Inactivation of the lipopeptide antibiotic daptomycin by hydrolytic mechanisms
    Vanessa M D'Costa
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry, and Biomedical Sciences, McMaster University, Hamilton, ON, Canada
    Antimicrob Agents Chemother 56:757-64. 2012
    ....
  10. doi request reprint Making sense of antisense in antibiotic drug discovery
    Gerard D Wright
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, ON, Canada
    Cell Host Microbe 6:197-8. 2009
    ..In an era of increasing antibiotic resistance, growing clinical need for new drugs, and few lead molecules, new research is helping to identify new targets and the mechanism of action of lead molecules...
  11. ncbi request reprint The antibiotic resistome: the nexus of chemical and genetic diversity
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main Street West Hamilton, Ontario, L8N 3Z5, Canada
    Nat Rev Microbiol 5:175-86. 2007
    ..Why is resistance inevitable and where does it come from? Understanding the molecular diversity that underlies resistance will inform our use of these drugs and guide efforts to develop new efficacious antibiotics...
  12. ncbi request reprint Mechanisms of resistance to antibiotics
    Gerard D Wright
    Department of Biochemistry, McMaster University, 1200 Main St W, Hamilton, Ontario L8N 3Z5, Canada
    Curr Opin Chem Biol 7:563-9. 2003
    ....
  13. ncbi request reprint On the road to bacterial cell death
    Gerard D Wright
    Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada
    Cell 130:781-3. 2007
    ..In this issue, Kohanski et al. (2007) provide evidence that the production of reactive oxygen species is a shared mechanism of cell death initiated by bactericidal antibiotics...
  14. ncbi request reprint New strategies for combating multidrug-resistant bacteria
    Gerard D Wright
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main St W, Hamilton, Ontario, L8N 3Z5, Canada
    Trends Mol Med 13:260-7. 2007
    ..This new chemistry, coupled with a growing understanding of the mechanisms, origins and distribution of antibiotic resistance, position us to tackle the challenges of antibiotic resistance in the 21st century...
  15. doi request reprint Chemical biology of tetracycline antibiotics
    Bijan Zakeri
    Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main St W, Hamilton, ON L8N3Z5, Canada
    Biochem Cell Biol 86:124-36. 2008
    ..Herein we discuss recent findings that have clarified the mode of action and the biosynthetic pathway of tetracyclines and that have shed light on the chemical biology of tetracycline antibiotics...
  16. ncbi request reprint Streptogramin antibiotics: mode of action and resistance
    Nicole J Johnston
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, ON, Canada
    Curr Drug Targets 3:335-44. 2002
    ..This review describes the current understanding of streptogramin function and resistance with emphasis on molecular mechanism and epidemiology...
  17. doi request reprint Isolation of flavonoids from the heartwood and resin of Prunus avium and some preliminary biological investigations
    James McNulty
    Department of Chemistry and Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4M1
    Phytochemistry 70:2040-6. 2009
    ..The defensive role of these natural plant flavonoids as antifungal phytoalexins and phytoanticipins is discussed...
  18. doi request reprint Novel approaches to discovery of antibacterial agents
    Patricia L Taylor
    Michael G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St W, Hamilton, ON L8N3Z5, Canada
    Anim Health Res Rev 9:237-46. 2008
    ..Finally, inhibiting non-essential genes with small molecules is being explored as a method for rescuing the activity of 'old' antibiotics, providing a novel synergistic approach to antimicrobial discovery...
  19. ncbi request reprint Chimeric streptogramin-tyrocidine antibiotics that overcome streptogramin resistance
    Tariq A Mukhtar
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street, West Hamilton, Ontario, L8N 3Z5, Canada
    Chem Biol 12:229-35. 2005
    ..These results allow for the development of a brand new class of antibiotics with the ability to evade type B streptogramin-resistance mechanisms...
  20. ncbi request reprint Glycopeptide antibiotic resistance
    Jeff Pootoolal
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
    Annu Rev Pharmacol Toxicol 42:381-408. 2002
    ....
  21. ncbi request reprint Inhibitors of bacterial cystathionine beta-lyase: leads for new antimicrobial agents and probes of enzyme structure and function
    Linda J Ejim
    Antimicrobial Research Centre, McMaster High Throughput Screening Laboratory, Department of Biochemistry and Biomedical Sciences, McMaster University, Ontario L8N 3Z5, Canada
    J Med Chem 50:755-64. 2007
    ..These studies provide the first lead molecules for antimicrobial agents that target cystathionine beta-lyase in methionine biosynthesis...
  22. ncbi request reprint Enzyme-assisted suicide: molecular basis for the antifungal activity of 5-hydroxy-4-oxonorvaline by potent inhibition of homoserine dehydrogenase
    Suzanne L Jacques
    Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton L8N 3Z5, Canada
    Chem Biol 10:989-95. 2003
    ..These findings clarify the apparent paradox of the potent antifungal actions of HON given its weak steady-state inhibition characteristics...
  23. ncbi request reprint Functional annotation of putative aminoglycoside antibiotic modifying proteins in Mycobacterium tuberculosis H37Rv
    Kari Ann Draker
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5
    J Antibiot (Tokyo) 56:135-42. 2003
    ..This study demonstrates the necessity of biochemical annotation methods as a follow up to in silico sequence alignment-based methods of assigning gene product function...
  24. ncbi request reprint Broad-spectrum peptide inhibitors of aminoglycoside antibiotic resistance enzymes
    David D Boehr
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 10:189-96. 2003
    ..These peptides represent the first example of broad-spectrum inhibitors of aminoglycoside resistance enzymes...
  25. ncbi request reprint Sampling the antibiotic resistome
    Vanessa M D'Costa
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Ontario, Canada, L8N 3Z5
    Science 311:374-7. 2006
    ..They are a reservoir of resistance determinants that can be mobilized into the microbial community. Study of this reservoir could provide an early warning system for future clinically relevant antibiotic resistance mechanisms...
  26. ncbi request reprint StoPK-1, a serine/threonine protein kinase from the glycopeptide antibiotic producer Streptomyces toyocaensis NRRL 15009, affects oxidative stress response
    John M Neu
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main St W, Hamilton, ON, Canada, L8N 3Z5
    Mol Microbiol 44:417-30. 2002
    ..These results broaden the roles of Ser/Thr protein kinases in bacteria and underscore the diversity of signal transduction mechanisms available to respond to various stimuli...
  27. ncbi request reprint Expanding the soil antibiotic resistome: exploring environmental diversity
    Emma Griffiths
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main St W, Hamilton, Ontario, Canada L8N 3Z5
    Curr Opin Microbiol 10:481-9. 2007
    ..This challenges our current understanding of antibiotic resistance and provides both barriers and opportunities for antimicrobial drug discovery...
  28. doi request reprint Induction of antimicrobial activities in heterologous streptomycetes using alleles of the Streptomyces coelicolor gene absA1
    Nancy L McKenzie
    Department of Biochemistry and Biomedical Sciences, MG DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada
    J Antibiot (Tokyo) 63:177-82. 2010
    ..As a result, we identified new, broad-spectrum antimicrobial activities for pulvomycin, including a potent antimicrobial activity against highly antibiotic-resistant Gram-negative and Gram-positive pathogens...
  29. ncbi request reprint New targets and screening approaches in antimicrobial drug discovery
    Eric D Brown
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
    Chem Rev 105:759-74. 2005
  30. ncbi request reprint Streptogramins, oxazolidinones, and other inhibitors of bacterial protein synthesis
    Tariq A Mukhtar
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
    Chem Rev 105:529-42. 2005
  31. ncbi request reprint Structure and function of sedoheptulose-7-phosphate isomerase, a critical enzyme for lipopolysaccharide biosynthesis and a target for antibiotic adjuvants
    Patricia L Taylor
    Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada
    J Biol Chem 283:2835-45. 2008
    ..We postulate GmhA acts through an enediol-intermediate isomerase mechanism...
  32. pmc Glycopeptide sulfation evades resistance
    Lindsay Kalan
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada
    J Bacteriol 195:167-71. 2013
    ..Our results begin to unravel the mystery of the biological role of glycopeptide sulfation and offer a potential new strategy for the development of new antibiotics that avoid resistance...
  33. pmc Cystathionine beta-lyase is important for virulence of Salmonella enterica serovar Typhimurium
    Linda J Ejim
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    Infect Immun 72:3310-4. 2004
    ..This result confirms a previous chemical validation of the Met biosynthetic pathway as a target for the development of antibacterial agents and demonstrates that cystathionine beta-lyase is important for bacterial virulence...
  34. doi request reprint Structure and function of the glycopeptide N-methyltransferase MtfA, a tool for the biosynthesis of modified glycopeptide antibiotics
    Rong Shi
    Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada
    Chem Biol 16:401-10. 2009
    ..Computational docking and molecular dynamics simulations were used to model binding of demethyl-vancomycin aglycone to MtfA. These results demonstrate its utility as a tool for engineering methylated analogs of GPAs...
  35. ncbi request reprint Multicopy suppressors for novel antibacterial compounds reveal targets and drug efflux susceptibility
    Xiaoming Li
    Department of Biochemistry, Antimicrobial Research Centre, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 11:1423-30. 2004
    ..Two leads, which produced clones containing the gene folA, encoding dihydrofolate reductase (DHFR), proved to target DHFR in vivo and were competitive inhibitors in vitro...
  36. pmc Nucleotide selectivity of antibiotic kinases
    Tushar Shakya
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Antimicrob Agents Chemother 54:1909-13. 2010
    ..These differences reveal likely different pathways in antibiotic resistance enzyme evolution and can be exploited in selective inhibitor design to counteract resistance...
  37. ncbi request reprint Simultaneous in vitro assay of the first four enzymes in the fungal aspartate pathway identifies a new class of aspartate kinase inhibitor
    David C Bareich
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street, West Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 10:967-73. 2003
  38. pmc Noncanonical vancomycin resistance cluster from Desulfitobacterium hafniense Y51
    Lindsay Kalan
    Michael G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street W, Hamilton, Ontario, Canada
    Antimicrob Agents Chemother 53:2841-5. 2009
    ..This finding represents a new glycopeptide antibiotic resistance gene cluster and expands the genetic diversity of resistance to this important class of antibiotic...
  39. ncbi request reprint Molecular mechanism of the enterococcal aminoglycoside 6'-N-acetyltransferase': role of GNAT-conserved residues in the chemistry of antibiotic inactivation
    Kari Ann Draker
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 43:446-54. 2004
    ....
  40. doi request reprint Structural and kinetic characterization of the LPS biosynthetic enzyme D-alpha,beta-D-heptose-1,7-bisphosphate phosphatase (GmhB) from Escherichia coli
    Patricia L Taylor
    Department of Biochemistry and Biomedical Sciences and M G DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 49:1033-41. 2010
    ..This study provides insight into the structure-function relationship of GmhB, a new target for combatting gram-negative bacterial infection...
  41. ncbi request reprint New phenolic inhibitors of yeast homoserine dehydrogenase
    Linda Ejim
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5
    Bioorg Med Chem 12:3825-30. 2004
    ..These results provide the first nonamino acid inhibitors of this class of enzyme and have the potential to be exploited as leads in antifungal compound design...
  42. pmc Rifamycin antibiotic resistance by ADP-ribosylation: Structure and diversity of Arr
    Jennifer Baysarowich
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada L8N 3Z5
    Proc Natl Acad Sci U S A 105:4886-91. 2008
    ..This work highlights the extent of the rifamycin resistome in microbial genera with the potential to negatively impact the expanded use of this class of antibiotic...
  43. doi request reprint Receptor domains of two-component signal transduction systems
    Julie Perry
    MG DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main St W, Hamilton, Ontario, Canada
    Mol Biosyst 7:1388-98. 2011
    ....
  44. doi request reprint The tetracycline resistome
    Maulik Thaker
    Department of Biochemistry and Biomedical Sciences, Michael G DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada
    Cell Mol Life Sci 67:419-31. 2010
    ....
  45. ncbi request reprint Kinetic mechanism of the GCN5-related chromosomal aminoglycoside acetyltransferase AAC(6')-Ii from Enterococcus faecium: evidence of dimer subunit cooperativity
    Kari Ann Draker
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Ontario L8N 3Z5, Canada
    Biochemistry 42:6565-74. 2003
    ..Taken together, these results demonstrate subunit cooperativity in the AAC(6')-Ii dimer, with possible relevance to other oligomeric members of the GNAT superfamily...
  46. ncbi request reprint Mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: role of the nucleotide positioning loop
    Paul R Thompson
    Department of Biochemistry, Antimicrobial Research Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    Biochemistry 41:7001-7. 2002
    ..The APH NPL therefore acts as a lever, promoting phosphoryl transfer to the aminoglycoside substrate, with the biological outcome of clinically relevant antibiotic resistance...
  47. ncbi request reprint One-pot chemoenzymatic preparation of coenzyme A analogues
    Ishac Nazi
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Ontario, Canada L8N 3Z5
    Anal Biochem 324:100-5. 2004
    ..The synthesis of five novel CoA derivatives is reported and the method is shown to be robust and tolerant of a number of different pantothenic acid structures, which indicates that the procedure should be widely applicable...
  48. ncbi request reprint Small molecule functional discrimination of the kinases required for the microbial synthesis of threonine and isoleucine
    David Bareich
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street W, Hamilton, Ontario, Canada L8N 3Z5
    Bioorg Med Chem 12:807-15. 2004
    ..These bisubstrate compounds only inhibited the bacterial aspartate kinase. These results reveal unexpected differences in small molecule interactions among these functionally similar enzymes...
  49. ncbi request reprint TetX is a flavin-dependent monooxygenase conferring resistance to tetracycline antibiotics
    Wangrong Yang
    Antimicrobial Research Center, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    J Biol Chem 279:52346-52. 2004
    ..This is the first molecular characterization of an antibiotic-inactivating monooxygenase, the origins of which may lie in environmental bacteria...
  50. ncbi request reprint Tigecycline is modified by the flavin-dependent monooxygenase TetX
    Ian F Moore
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, L8N 3Z5, Canada
    Biochemistry 44:11829-35. 2005
    ....
  51. ncbi request reprint Biosynthesis of sulfated glycopeptide antibiotics by using the sulfotransferase StaL
    Sherry S Lamb
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Chem Biol 13:171-81. 2006
    ..This unique modification by sulfation expands glycopeptide diversity with potential application for the development of new antibiotics...
  52. ncbi request reprint An in vitro screen of bacterial lipopolysaccharide biosynthetic enzymes identifies an inhibitor of ADP-heptose biosynthesis
    Gladys P De Leon
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Chem Biol 13:437-41. 2006
    ....
  53. pmc Role of homoserine transacetylase as a new target for antifungal agents
    Ishac Nazi
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Antimicrob Agents Chemother 51:1731-6. 2007
    ..This work validates HTA as an attractive drug-susceptible target for new antifungal agent design...
  54. doi request reprint Structure and mechanism of the lincosamide antibiotic adenylyltransferase LinB
    Mariya Morar
    M G DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences and the Department of Chemistry, McMaster University, Hamilton, ON L8N 3Z5, Canada
    Structure 17:1649-59. 2009
    ..The LinB structure provides an evolutionary link to ancient nucleotide polymerases and suggests that, like protein kinases and acetyltransferases, these are proto-resistance elements from which drug resistance can evolve...
  55. ncbi request reprint Establishing the principles of recognition in the adenine-binding region of an aminoglycoside antibiotic kinase [APH(3')-IIIa]
    David D Boehr
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Biochemistry 44:12445-53. 2005
    ..The principles governing adenine recognition established in this study may be applied to other protein-ligand complexes and used to navigate future studies directed at discovering potent and selective inhibitors of APH-type enzymes...
  56. pmc Accessorizing natural products: adding to nature's toolbox
    Sherry S Lamb
    Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5
    Proc Natl Acad Sci U S A 102:519-20. 2005
  57. pmc Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009
    Jeff Pootoolal
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, ON, Canada L8N 3Z5
    Proc Natl Acad Sci U S A 99:8962-7. 2002
    ..These results provide increased understanding of the biosynthesis of these complex natural products...
  58. ncbi request reprint The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of ASP-99 as an active site base important for acetyl transfer
    David D Boehr
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    J Biol Chem 278:12873-80. 2003
    ..The prominent role of this residue in aminoglycoside modification can be exploited as an anchoring site for the development of compounds capable of reversing antibiotic resistance in vivo...
  59. ncbi request reprint Functionally important amino acids in Saccharomyces cerevisiae aspartate kinase
    David C Bareich
    Department of Biochemistry, McMaster University, 1200 Main St, W, Hamilton, Ont, Canada L8N 3Z5
    Biochem Biophys Res Commun 311:597-603. 2003
    ..These results provide the first identification of amino acid residues crucial to the action of this important metabolic enzyme...
  60. ncbi request reprint Crystal structure of homoserine transacetylase from Haemophilus influenzae reveals a new family of alpha/beta-hydrolases
    I Ahmad Mirza
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3A 1A4
    Biochemistry 44:15768-73. 2005
    ..Furthermore, the properties of the tunnel provide a rationale for how homoserine transacetylase catalyzes a transferase reaction vs hydrolysis, despite extensive similarity in active site architecture to hydrolytic enzymes...
  61. ncbi request reprint Crystal structure of StaL, a glycopeptide antibiotic sulfotransferase from Streptomyces toyocaensis
    Rong Shi
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6
    J Biol Chem 282:13073-86. 2007
    ....
  62. ncbi request reprint Characterization of an inducible vancomycin resistance system in Streptomyces coelicolor reveals a novel gene (vanK) required for drug resistance
    Hee Jeon Hong
    Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, UK
    Mol Microbiol 52:1107-21. 2004
    ..Analysis of mutants defective in the vanRS and cseBC cell envelope signal transduction systems revealed significant cross-talk between the two pathways...
  63. ncbi request reprint Synthesis and structure-activity relationships of truncated bisubstrate inhibitors of aminoglycoside 6'-N-acetyltransferases
    Feng Gao
    Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 2K6, Canada
    J Med Chem 49:5273-81. 2006
    ..Moreover, one of the inhibitors is able to block aminoglycoside resistance in cells expressing this enzyme...
  64. pmc Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase
    Magdalena Korczynska
    Department of Biochemistry, McGill University, Montreal, QC, Canada H3A 4A2
    Proc Natl Acad Sci U S A 104:10388-93. 2007
    ....