Research Topics
Genomes and GenesSpecies
| R S GuptaSummaryAffiliation: McMaster University Country: Canada Publications
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Detail Information
Publications
What are archaebacteria: life's third domain or monoderm prokaryotes related to gram-positive bacteria? A new proposal for the classification of prokaryotic organismsR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Mol Microbiol 29:695-707. 1998..These observations provide evidence for an alternate view of the evolutionary relationship among living organisms that is different from the currently popular three-domain proposal...- Evolutionary relationships among photosynthetic prokaryotes (Heliobacterium chlorum, Chloroflexus aurantiacus, cyanobacteria, Chlorobium tepidum and proteobacteria): implications regarding the origin of photosynthesisR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
Mol Microbiol 32:893-906. 1999..Some implications of these results concerning the origin of photosynthesis and the earliest prokaryotic fossils are discussed... - The natural evolutionary relationships among prokaryotesR S Gupta
Department of Biochemistry, McMaster University Hamilton, Ontario, Canada
Crit Rev Microbiol 26:111-31. 2000.... - Life's third domain (Archaea): an established fact or an endangered paradigm?R S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, L8N 3Z5, Canada
Theor Popul Biol 54:91-104. 1998..A new classifica- tion of organisms consistent with phenotype and macromolecular sequence data is proposed... - The phylogeny of proteobacteria: relationships to other eubacterial phyla and eukaryotesR S Gupta
Department of Biochemistry, McMaster University, L8N 3Z5, Hamilton, Ont, Canada
FEMS Microbiol Rev 24:367-402. 2000..These signatures generally support the origin of mitochondria from an alpha-proteobacterium and provide evidence that the nuclear cytosolic homologs of many genes are also derived from proteobacteria... - Cloning and some novel characteristics of mitochondrial Hsp70 from Chinese hamster cellsB Singh
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Exp Cell Res 234:205-16. 1997..These studies raise the possibility that similar to the Hsp60 chaperonin and a number of other mitochondrial proteins, mHsp70 may have an extramitochondrial role... - The sequences of heat shock protein 40 (DnaJ) homologs provide evidence for a close evolutionary relationship between the Deinococcus-thermus group and cyanobacteriaK Bustard
Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada
J Mol Evol 45:193-205. 1997..However, in phylogenetic trees based on Hsp40 sequences, the two archaebacterial homologs showed a polyphyletic branching within Gram-positive bacteria, similar to that seen with Hsp70 sequences... - Phylogenetic analysis of mycoplasmas based on Hsp70 sequences: cloning of the dnaK (hsp70) gene region of Mycoplasma capricolumM Falah
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Int J Syst Bacteriol 47:38-45. 1997..The phylogenetic trees based on Hsp70 sequences show a polyphyletic branching of archaebacteria with the gram-postive species, which is statistically strongly favored... - A novel ubiquitous protein 'chaperonin' supports the endosymbiotic origin of mitochondrion and plant chloroplastR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Biochem Biophys Res Commun 163:780-7. 1989..The sequence data for this protein thus support the contention that the endosymbiont that gave rise to mitochondrion was a member of purple bacteria, while plant chloroplast originated from a member of the cyanobacterial lineage... - Evolution of HSP70 gene and its implications regarding relationships between archaebacteria, eubacteria, and eukaryotesR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Canada
J Mol Evol 37:573-82. 1993..To explain the phylogenies based on HSP70 and other gene sequences, a model for the origin of eukaryotic cells involving fusion between archaebacteria and gram-negative eubacteria is proposed... - Adenosine-AMP exchange activity is an integral part of the mammalian adenosine kinaseR S Gupta
Department of Biochemistry, McMaster University Hamilton, Ontario, Canada
Biochem Mol Biol Int 39:493-502. 1996..Some implications of these observations regarding the catalytic mechanism of AK are discussed... - Cloning and characterization of multiple groEL chaperonin-encoding genes in Rhizobium melilotiE Rusanganwa
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Gene 126:67-75. 1993..The presence of multiple GroEL homologues in R. meliloti suggests a possible role of the GroEL or HSP60 chaperonins in the nodulation (symbiosis) and nitrogen fixation processes... 
Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigenS Jindal
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Mol Cell Biol 9:2279-83. 1989..The observed high degree of homology between human P1 and mycobacterial antigen also suggests the possible involvement of this protein in certain autoimmune diseases...- Gene structure for adenosine kinase in Chinese hamster and human: high-frequency mutants of CHO cells involve deletions of several introns and exonsB Singh
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
DNA Cell Biol 20:53-65. 2001..The endpoints of these deletions lie in the large introns within the AK gene... - Cloning of HSP70 (dnaK) gene from Clostridium perfringens using a general polymerase chain reaction based approachK A Galley
Department of Biochemistry, McMaster University, Hamilton, Canada
Biochim Biophys Acta 1130:203-8. 1992..The HSP70s from C. perfringens as well as other Gram-positive groups of bacteria contain a large deletion of 25 amino acids, near the N-terminal end, which is not seen in HSP70 from other sources... - Molecular cloning of a Chinese hamster mitochondrial protein related to the "chaperonin" family of bacterial and plant proteinsD J Picketts
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
J Biol Chem 264:12001-8. 1989..These observations strongly indicate that P1 protein is a member of the chaperonin family and that it may be involved in a similar function in mammalian cells... - Phylogenetic analysis of 70 kD heat shock protein sequences suggests a chimeric origin for the eukaryotic cell nucleusR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Curr Biol 4:1104-14. 1994.... - Protein phylogenies and signature sequences: A reappraisal of evolutionary relationships among archaebacteria, eubacteria, and eukaryotesR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
Microbiol Mol Biol Rev 62:1435-91. 1998..In this model, the ancestral eukaryotic cell is a chimera that resulted from a unique fusion event between the two separate groups of prokaryotes followed by integration of their genomes... - Immunoelectron microscopic localization of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian cellsB J Soltys
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Exp Cell Res 222:16-27. 1996..These findings raise interesting questions concerning the possible role of Hsp60 at extramitochondrial sites... - The use of signature sequences in different proteins to determine the relative branching order of bacterial divisions: evidence that Fibrobacter diverged at a similar time to Chlamydia and the Cytophaga-Flavobacterium-Bacteroides divisionE Griffiths
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Microbiology 147:2611-22. 2001..succinogenes homologues. These results provide evidence that F. succinogenes does not belong to the proteobacterial division and thus should be placed in a similar position as the Chlamydia and CFB groups of species... - Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequenceM Falah
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
J Bacteriol 176:7748-53. 1994..The analysis presented here also suggests a monophyletic origin of the mitochondrial homologs... - Cloning of the HSP70 gene from Halobacterium marismortui: relatedness of archaebacterial HSP70 to its eubacterial homologs and a model for the evolution of the HSP70 geneR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
J Bacteriol 174:4594-605. 1992..mazei) and the gram-positive group of bacteria constitutes the ancestral form of the protein and that all other HSP70s (viz., other eubacteria as well as eukaryotes) containing the insert have evolved from this ancient protein... - Identification of a protein altered in mutants resistant to microtubule inhibitors as a member of the major heat shock protein (hsp70) familyS Ahmad
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Mol Cell Biol 10:5160-5. 1990..The fact that there is an alteration in hsc70 in mutants resistant to antimitotic drugs suggests a role for this protein in the in vivo assembly and function of microtubules... - The effect of inorganic phosphate on the activity of bacterial ribokinaseM C Maj
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
J Protein Chem 20:139-44. 2001..The similar effect of PVI on distantly related enzymes suggests that a common mechanism for activity is shared among PfkB family members... - Structure-activity studies on mammalian adenosine kinaseM C Maj
Department of Biochemistry, McMaster University, Hamilton, Ontario, L8N 3Z5, Canada
Biochem Biophys Res Commun 275:386-93. 2000.... - The branching order and phylogenetic placement of species from completed bacterial genomes, based on conserved indels found in various proteinsR S Gupta
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Int Microbiol 4:187-202. 2001.... 
Adenosine kinase and ribokinase--the RK family of proteinsJ Park
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada L8N 3Z5
Cell Mol Life Sci 65:2875-96. 2008..The cellular metabolism and transport of ribose and adenosine are also briefly discussed, as well as the beneficial effects of ribose and adenosine in physiology and how these effects can be harnessed for pharmacological purposes...- Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene familiesT J Venner
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
DNA Cell Biol 9:545-52. 1990..The sequence data, however, suggest that most of these genes, except one (per haploid genome), are likely to be nonfunctional pseudogenes... - Nucleotide sequences of three different isoforms of beta-tubulin cDNA from Chinese hamster ovary cellsS Ahmad
Department of Biochemistry, McMaster University, Hamilton, Canada
Biochim Biophys Acta 1090:252-4. 1991..These results provide evidence for the expression of three different isoforms of beta-tubulin in Chinese hamster ovary cells... - Cloning of a Chinese hamster protein homologous to the mouse t-complex protein TCP-1: structural similarity to the ubiquitous 'chaperonin' family of heat-shock proteinsS Ahmad
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Biochim Biophys Acta 1087:253-5. 1990..The deduced amino acid sequences of the mouse/Chinese hamster TCP-1 proteins exhibit significant identity to the 60-65 kDa heat shock 'chaperonin' family of proteins present in prokaryotes and in the eukaryotic cell organelles... - Nucleotide sequence of rat hsp60 (chaperonin, GroEL homolog) cDNAT J Venner
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Nucleic Acids Res 18:5309. 1990 - The influence of inorganic phosphate on the activity of adenosine kinaseM Maj
Department of Biochemistry, McMaster University, Hamilton, Ont, Canada
Biochim Biophys Acta 1476:33-42. 2000..The effect that phosphate exerts on AK may be either to protect the enzyme from inactivation at high adenosine and H(+) concentrations or to stabilize substrate binding at the active site... - Immunogold localization of mitochondrial aspartate aminotransferase in mitochondria and on the cell surface in normal rat tissuesJ D Cechetto
Department of Biochemistry, McMaster University, Hamilton, Canada
Histol Histopathol 17:353-64. 2002..These results have implications for the role of mitochondria in apoptosis and different diseases... - Cytochrome-C localizes in secretory granules in pancreas and anterior pituitaryB J Soltys
Department of Biochemistry, McMaster University, Hamilton, Ontario, L8N 3Z5, Canada
Cell Biol Int 25:331-8. 2001..The indicated presence of cytochrome-c outside mitochondria in certain tissues under normal physiological conditions raises interesting questions concerning translocation mechanisms and the cellular functions of cytochrome-c... - Immunoelectron microscopy provides evidence for the presence of mitochondrial heat shock 10-kDa protein (chaperonin 10) in red blood cells and a variety of secretory granulesS K Sadacharan
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8 N 3Z5
Histochem Cell Biol 116:507-17. 2001..The presence of these proteins at extramitochondrial sites in normal tissues has important implications concerning the role of mitochondria in apoptosis and genetic diseases... - Nucleotide sequence of mouse HSP60 (chaperonin, GroEL homolog) cDNAT J Venner
Department of Biochemistry, McMaster University, Hamilton, Canada
Biochim Biophys Acta 1087:336-8. 1990..The deduced amino acid sequence of mouse hsp60 protein differs from the corresponding proteins from Chinese hamster and human cells in 7 and 13 residues, respectively, most of which are conservative replacements... - Mitochondrial-matrix proteins at unexpected locations: are they exported?B J Soltys
Dept of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
Trends Biochem Sci 24:174-7. 1999..We suggest that mitochondria, as organelles of bacterial origin, possess specific mechanisms for export of proteins to other compartments... - Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the geneB Singh
Department of Biochemistry, McMaster University, Hamilton, Canada
Eur J Biochem 241:564-71. 1996..The sequence similarity data support the possibility that adenosine kinase shares a common evolutionary ancestor with these protein sequences... - Intestinal expression and cellular immune responses to human heat-shock protein 60 in Crohn's diseaseM E Baca-Estrada
Department of Medicine, McMaster University, Hamilton, Ontario, Canada
Dig Dis Sci 39:498-506. 1994..These results suggest that endogenous human HSP60 is unlikely to be a target for an autoimmune response in patients with Crohn's disease... - Mitochondrial import of the human chaperonin (HSP60) proteinB Singh
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Biochem Biophys Res Commun 169:391-6. 1990..ornithine carbamoyltransferase and uncoupling protein) much less binding of pre P1 to mitochondria was observed. The significance of this latter observation at present is unclear... - Induction of Hsp60 by Photofrin-mediated photodynamic therapyJ G Hanlon
Hamilton Regional Cancer Centre, 699 Concession Street, ON, L8V 5C2, Hamilton, Canada
J Photochem Photobiol B 64:55-61. 2001..These results indicate that the presence of PII and the subsequent oxidative stress of PDT can induce Hsp60 and implicated it as a common factor that may contribute to the resistance observed in the induced resistant cells... - Investigation of the relationship between altered intracellular pH and multidrug resistance in mammalian cellsD Boscoboinik
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Br J Cancer 61:568-72. 1990..Therefore, except for the effect of cyclosporin A on the CHRC5 line, the effects of other agents on reversal of multidrug resistance and intracellular pH did not correlate with each other... - Cloning of HSP60 (GroEL) operon from Clostridium perfringens using a polymerase chain reaction based approachE Rusanganwa
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Biochim Biophys Acta 1130:90-4. 1992..The approach described here, employing this set of degenerate oligonucleotide primers, could be used to clone HSP60 gene/cDNA from any species... - Cell surface localization of the 60 kDa heat shock chaperonin protein (hsp60) in mammalian cellsB J Soltys
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Cell Biol Int 21:315-20. 1997..The finding of hsp60 on the cell surface of mammalian cells may signify chaperone involvement in surface functions... - Reversal of intrinsic multidrug resistance in Chinese hamster ovary cells by amiloride analogsR F Epand
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
Br J Cancer 63:247-51. 1991..The differential effect of amiloride analogs on intrinsic versus acquired MDR characteristics of Chinese hamster cells suggests some differences in the underlying resistance mechanisms... - Hypocholesterolaemic effect of the oleoresin of Capsicum annum L. in gerbils (Meriones hurrianae Jerdon)R S Gupta
Department of Zoology, University of Rajasthan, Jaipur, India
Phytother Res 16:273-5. 2002..CO feeding prevented the accumulation of cholesterol and triglycerides in the liver and aorta. The faecal excretion of cholesterol and triglycerides were significantly increased in oleoresin fed gerbils... - Comparative protein profiles of Salmonella and Escherichia coliMeenal Gupta
Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, G B Pant University of Agriculture and Technology, Pantnagar 263 145, India
Indian J Exp Biol 43:475-8. 2005..It was found that S. Weltevreden shared 7 bands with E. coli O78, A protein of molecular weight 20.89 kDa was found in all Salmonella serovars, but not in E. coli O78 suggesting its genus specific attribute...