P Roughley

Summary

Affiliation: McGill University
Country: Canada

Publications

  1. pmc The efficacy of Link N as a mediator of repair in a rabbit model of intervertebral disc degeneration
    Fackson Mwale
    Division of Orthopaedic Surgery, McGill University, 1650 Cedar Avenue, Montreal, QC, Canada, H3G 1A4
    Arthritis Res Ther 13:R120. 2011
  2. pmc Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degenerate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues
    James Melrose
    Raymond Purves Research Laboratory, Institute of Bone and Joint Research, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Leonards, NSW, Australia
    Arthritis Res Ther 10:R79. 2008
  3. pmc Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site
    Jordi Monfort
    Osteoarthritis Research Unit, University of Montreal Hospital Centre, Notre Dame Hospital, 1560 Sherbrooke Street East, Montreal, Quebec H2L 4M1, Canada
    Arthritis Res Ther 8:R26. 2006
  4. ncbi request reprint Biology of intervertebral disc aging and degeneration: involvement of the extracellular matrix
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Spine (Phila Pa 1976) 29:2691-9. 2004
  5. doi request reprint The role of hyaluronan produced by Has2 gene expression in development of the spine
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada
    Spine (Phila Pa 1976) 36:E914-20. 2011
  6. ncbi request reprint The structure and function of cartilage proteoglycans
    P J Roughley
    Genetics Unit, Shriners Hospital for Children and Department of Surgery, McGill University, Montreal, Quebec, Canada
    Eur Cell Mater 12:92-101. 2006
  7. ncbi request reprint The role of proteoglycans in aging, degeneration and repair of the intervertebral disc
    P J Roughley
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, H3G 1A6, Canada
    Biochem Soc Trans 30:869-74. 2002
  8. ncbi request reprint The involvement of aggrecan polymorphism in degeneration of human intervertebral disc and articular cartilage
    P Roughley
    Shriners Hospital for Children, Department of Surgery, McGill University, Montreal, Quebec, Canada
    Eur Cell Mater 11:1-7; discussion 7. 2006
  9. pmc The structure and degradation of aggrecan in human intervertebral disc
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, QC, Canada
    Eur Spine J 15:S326-32. 2006
  10. pmc Variations in aggrecan structure modulate its susceptibility to aggrecanases
    Peter J Roughley
    Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Biochem J 375:183-9. 2003

Collaborators

Detail Information

Publications77

  1. pmc The efficacy of Link N as a mediator of repair in a rabbit model of intervertebral disc degeneration
    Fackson Mwale
    Division of Orthopaedic Surgery, McGill University, 1650 Cedar Avenue, Montreal, QC, Canada, H3G 1A4
    Arthritis Res Ther 13:R120. 2011
    ..The purpose of the present study was to evaluate the effect of Link N in vivo in a rabbit model of IVD degeneration...
  2. pmc Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degenerate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues
    James Melrose
    Raymond Purves Research Laboratory, Institute of Bone and Joint Research, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Leonards, NSW, Australia
    Arthritis Res Ther 10:R79. 2008
    ..Catabolism of SLRPs has important consequences for the integrity of articular cartilage and meniscus by interfering with their tissue homeostatic functions...
  3. pmc Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site
    Jordi Monfort
    Osteoarthritis Research Unit, University of Montreal Hospital Centre, Notre Dame Hospital, 1560 Sherbrooke Street East, Montreal, Quebec H2L 4M1, Canada
    Arthritis Res Ther 8:R26. 2006
    ..Awareness of SLRP degradation products, especially those of biglycan and fibromodulin, may assist in early detection of OA cartilage degradation...
  4. ncbi request reprint Biology of intervertebral disc aging and degeneration: involvement of the extracellular matrix
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Spine (Phila Pa 1976) 29:2691-9. 2004
    ..A review of current knowledge and opinions concerning the biologic changes that take place during development, maturation and degeneration of the intervertebral disc...
  5. doi request reprint The role of hyaluronan produced by Has2 gene expression in development of the spine
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada
    Spine (Phila Pa 1976) 36:E914-20. 2011
    ..Histologic analysis of spine development in cartilage-specific knockout mice...
  6. ncbi request reprint The structure and function of cartilage proteoglycans
    P J Roughley
    Genetics Unit, Shriners Hospital for Children and Department of Surgery, McGill University, Montreal, Quebec, Canada
    Eur Cell Mater 12:92-101. 2006
    ..This review discusses the structure/function relationships of the cartilage proteoglycans, and the manner in which perturbations in proteoglycan structure or abundance can adversely affect tissue function...
  7. ncbi request reprint The role of proteoglycans in aging, degeneration and repair of the intervertebral disc
    P J Roughley
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, H3G 1A6, Canada
    Biochem Soc Trans 30:869-74. 2002
    ..Such cells can be supported in a biomolecular scaffold, but it is essential that the scaffold is compatible with high aggrecan retention if a functional tissue is to be attained...
  8. ncbi request reprint The involvement of aggrecan polymorphism in degeneration of human intervertebral disc and articular cartilage
    P Roughley
    Shriners Hospital for Children, Department of Surgery, McGill University, Montreal, Quebec, Canada
    Eur Cell Mater 11:1-7; discussion 7. 2006
    ....
  9. pmc The structure and degradation of aggrecan in human intervertebral disc
    Peter J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, QC, Canada
    Eur Spine J 15:S326-32. 2006
    ..The origin and spectrum of disc proteoglycan heterogeneity is the same in both the annulus fibrosus and nucleus pulposus...
  10. pmc Variations in aggrecan structure modulate its susceptibility to aggrecanases
    Peter J Roughley
    Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Biochem J 375:183-9. 2003
    ..Thus, for aggrecan cleavage by aggrecanases, variations in cleavage efficiency exist with respect to the species and age of the animal from which the aggrecan is derived and the type of aggrecanase being used...
  11. ncbi request reprint Osteogenesis imperfecta--clinical and molecular diversity
    P J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, Canada
    Eur Cell Mater 5:41-7; discussion 47. 2003
    ..Treatment of osteogenesis imperfecta by bisphosphonate therapy can improve bone mass in all types of the disorder, and while not being a cure for the disorder does improve the quality of life of the patient...
  12. ncbi request reprint The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation
    Peter Roughley
    Genetics Unit, Shriners Hospital for Children, and Department of Surgery, McGill University, Montreal, Canada
    Biomaterials 27:388-96. 2006
    ..The results support the concept that chitosan may be a suitable scaffold for cell-based supplementation to help restore the function of the NP during the early stages of IVD degeneration...
  13. pmc Articular cartilage and changes in arthritis: noncollagenous proteins and proteoglycans in the extracellular matrix of cartilage
    P J Roughley
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Arthritis Res 3:342-7. 2001
    ..This review provides a selective survey of these molecules and discusses their structure, function, and involvement in inherited and arthritic disorders...
  14. doi request reprint Analysis of aggrecan catabolism by immunoblotting and immunohistochemistry
    Peter J Roughley
    Research Unit, Shriners Hospital for Children, Montreal, QC, Canada
    Methods Mol Biol 836:219-37. 2012
    ....
  15. pmc Severe osteogenesis imperfecta caused by a small in-frame deletion in CRTAP
    I M Ben Amor
    Genetics Unit, Shriners Hospital for Children and McGill University, Montreal, Canada
    Am J Med Genet A 155:2865-70. 2011
    ..We are unaware of prior reports of this finding. We suggest that this deletion affects crucial amino acids that are important for the interaction and/or stabilization of CRTAP and P3H1...
  16. ncbi request reprint Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue engineering of intervertebral disc
    F Mwale
    Lady Davis Institute for Medical Research and Department of Surgery, SMBD Jewish General Hospital, Montreal, QC, Canada
    Eur Cell Mater 8:58-63; discussion 63-4. 2004
    ....
  17. ncbi request reprint Link protein can retard the degradation of hyaluronan in proteoglycan aggregates
    E Rodriguez
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Osteoarthritis Cartilage 14:823-9. 2006
    ..The aim of this work was to determine whether degradation of HA in PG aggregate degradation is influenced by link protein (LP) stabilization of the PG aggregates...
  18. ncbi request reprint Type V osteogenesis imperfecta: a new form of brittle bone disease
    F H Glorieux
    Genetics Unit, Shriners Hospital, Montreal, Quebec, Canada
    J Bone Miner Res 15:1650-8. 2000
    ..In conclusion, OI type V is a new form of autosomal dominant OI, which does not appear to be associated with collagen type I mutations. The genetic defect underlying this disease remains to be elucidated...
  19. pmc Differential regulation and expression of hyaluronan synthases in human articular chondrocytes, synovial cells and osteosarcoma cells
    A D Recklies
    Joint Diseases Laboratory, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, H3G 1A6, Canada
    Biochem J 354:17-24. 2001
    ..In addition, regulation of HA biosynthesis appears to be multi-faceted, with control of HAS gene expression and mRNA levels being only one aspect of this process...
  20. pmc A biological approach to treating disc degeneration: not for today, but maybe for tomorrow
    M Alini
    Orthopaedic Research Laboratory, Division of Orthopaedic Surgery, McGill University, Montreal, Canada
    Eur Spine J 11:S215-20. 2002
    ....
  21. ncbi request reprint Age-related changes in fibromodulin and lumican in human intervertebral discs
    R Sztrolovics
    Joint Diseases Laboratory, Shriners Hospital for Children, Montreal, Quebec, Canada
    Spine (Phila Pa 1976) 24:1765-71. 1999
    ..CONCLUSIONS: Growth, aging, and degeneration of the intervertebral disc are associated with changes in the abundance and structure of fibromodulin and lumican, which presumably influence the functional properties of the tissue...
  22. ncbi request reprint Localization of the human fibromodulin gene (FMOD) to chromosome 1q32 and completion of the cDNA sequence
    R Sztrolovics
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Canada
    Genomics 23:715-7. 1994
  23. ncbi request reprint The cleavage of biglycan by aggrecanases
    L I Melching
    Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada
    Osteoarthritis Cartilage 14:1147-54. 2006
    ..While this may be the preferred substrate for these enzymes, they are also able to degrade other proteins. The aim of this work was to determine whether the aggrecanases could degrade biglycan and decorin...
  24. ncbi request reprint Mechanism of proteoglycan aggregate degradation in cartilage stimulated with oncostatin M
    M Durigova
    Shriners Hospital for Children, Department of Surgery, McGill University, Montreal, Quebec, Canada
    Osteoarthritis Cartilage 16:98-104. 2008
    ..To investigate the potential synergistic and differential effects of cytokine combinations on proteoglycan aggregate catabolism in cartilage...
  25. ncbi request reprint Thirty-three novel COL1A1 and COL1A2 mutations in patients with osteogenesis imperfecta types I-IV
    L M Ward
    Genetics Unit, Shriners Hospital for Children, , , Canada
    Hum Mutat 17:434. 2001
    ..The variety of mutations described in the COL1A1/COL1A2 genes giving rise to an OI phenotype is in accordance with the clinical heterogeneity of the disease. Hum Mutat 17:434, 2001...
  26. pmc Development of a whole disc organ culture system to study human intervertebral disc
    M Parolin
    Department of Surgery, McGill University, Montreal, Quebec, Canada
    Evid Based Spine Care J 1:67-8. 2010
    ....
  27. doi request reprint Short- and long-term outcome of patients with pseudo-vitamin D deficiency rickets treated with calcitriol
    T Edouard
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    J Clin Endocrinol Metab 96:82-9. 2011
    ..The long-term (>1 yr) effects of calcitriol replacement treatment have not been reported...
  28. ncbi request reprint The consequence of PRELP overexpression on skin
    J Grover
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Matrix Biol 26:140-3. 2007
    ..A connective tissue phenotype was observed in the skin, where the organization of collagen fibrils in the dermis was perturbed and the thickness of the hypodermal fat layer was diminished...
  29. ncbi request reprint Cartilage proteoglycans: structure and potential functions
    P J Roughley
    Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    Microsc Res Tech 28:385-97. 1994
    ..Finally, type IX collagen can also be considered as a proteoglycan, as its alpha 2(IX) chain may bear a glycosaminoglycan chain. It may serve as a bridge between the collagen fibrils or with the interspersed aggrecan network...
  30. doi request reprint Characterization of an ADAMTS-5-mediated cleavage site in aggrecan in OSM-stimulated bovine cartilage
    M Durigova
    Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec H3G 1A6, Canada
    Osteoarthritis Cartilage 16:1245-52. 2008
    ....
  31. ncbi request reprint Identification of type I collagen gene polymorphisms: tolerance of sequence variation at an alpha 2(I) helix Y position
    R Sztrolovics
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Canada
    Matrix Biol 14:9-19. 1994
    ..The data suggest that at least some alpha 1(I) and alpha 2(I) helix Y positions may be tolerant of sequence variation, particularly if the replacing amino acid is proline, a residue involved in stabilizing the collagen triple helix...
  32. ncbi request reprint Osteogenesis imperfecta type VII: an autosomal recessive form of brittle bone disease
    L M Ward
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Bone 31:12-8. 2002
    ..The disease has subsequently been localized to chromosome 3p22-24.1, which is outside the loci for type I collagen genes. The underlying genetic basis for the disease remains to be determined...
  33. ncbi request reprint Identification of type I collagen gene (COL1A2) mutations in nonlethal osteogenesis imperfecta
    R Sztrolovics
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    Hum Mol Genet 2:1319-21. 1993
  34. ncbi request reprint The gene organization, chromosome location, and expression of a 55-kDa matrix protein (PRELP) of human articular cartilage
    J Grover
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    Genomics 38:109-17. 1996
    ..The presence of protein in the cartilage matrix was also much lower in the neonate than in the adult. In noncartilagenous tissues appreciable message levels were observed only in the adult lung...
  35. ncbi request reprint The structure and chromosome location of the human chondroadherin gene (CHAD)
    J Grover
    Department of Surgery, Shriners Hospital for Children, Montreal, Quebec, H3G 1A6, Canada
    Genomics 45:379-85. 1997
    ..Isolation of a cosmid clone spanning the chondroadherin gene enabled its chromosome location to be established. The gene was shown to reside at chromosome 17q21.33...
  36. ncbi request reprint The human lumican gene. Organization, chromosomal location, and expression in articular cartilage
    J Grover
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    J Biol Chem 270:21942-9. 1995
    ..In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan...
  37. ncbi request reprint Osteogenesis imperfecta: comparison of molecular defects with bone histological changes
    R Sztrolovics
    Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    Bone 15:321-8. 1994
    ..The results are compatible with there being a gradient in severity between OI types II and III, and that parameters external to the gene mutations might account for the survival differences in the 2 cases presented in this study...
  38. ncbi request reprint Changes in leucine-rich repeat proteoglycans during maturation of the bovine growth plate
    M Alini
    Orthopaedic Research Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada
    Matrix Biol 19:805-13. 2001
    ..In the case of both biglycan and fibromodulin, message expression continued at a similar level throughout the growth plate. Thus, the leucine-rich repeat proteoglycans are different in the way they behave during growth plate maturation...
  39. pmc Dermatan sulphate proteoglycans of human articular cartilage. The properties of dermatan sulphate proteoglycans I and II
    P J Roughley
    Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    Biochem J 262:823-7. 1989
    ..Sequence analysis of the N-terminal 20 amino acid residues reveals the presence of a single site for the potential substitution of dermatan sulphate at residue 4 of DS-PGII and two such sites at residues 5 and 10 for DS-PGI...
  40. pmc Characterization of the human proline/arginine-rich end leucine-rich repeat protein (PRELP) gene promoter and identification of a repressor element
    J Grover
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Biochem J 336:77-82. 1998
    ..The factor was not detected in nuclear protein preparations from adult chondrocytes in which the PRELP gene is expressed...
  41. ncbi request reprint Analysis of the human lumican gene promoter
    J Grover
    Genetics Unit, Shriners Hospital for Children and Department of Surgery, McGill University, Montreal, Quebec H3G 1A6, Canada
    J Biol Chem 275:40967-73. 2000
    ..The mouse lumican promoter does not possess an equivalent site, and this may explain why the lumican gene is expressed in fetal murine cartilage but not in fetal human cartilage...
  42. ncbi request reprint Characterization and expression of murine PRELP
    J Grover
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Matrix Biol 20:555-64. 2001
    ..The promoter region of the mouse PRELP gene possesses no TATA box in its proximal region, in common with humans, and shows differences in the conservation of elements known to be involved in regulating expression of the human PRELP gene...
  43. ncbi request reprint Osteogenesis imperfecta type VII maps to the short arm of chromosome 3
    M Labuda
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    Bone 31:19-25. 2002
    ..This study excludes type I collagen mutations in the pathogenesis of the disease and assigns this form of OI to a locus other than the ones containing the type I collagen genes...
  44. ncbi request reprint Differential regulation of matrix degrading enzymes in a TNFalpha-induced model of nucleus pulposus tissue degeneration
    Cheryle A Séguin
    BioEngineering of Skeletal Tissues Team, University of Toronto, Ontario, Canada
    Matrix Biol 25:409-18. 2006
    ..Depletion of tissue proteoglycans was mediated by ERK and NF-kappaB-dependent "aggrecanase" activity, suggesting MT1-MMP and ADAMTS-4 and -5 as effectors of TNFalpha-induced tissue catabolism...
  45. ncbi request reprint Proteoglycan expression in bleomycin lung fibroblasts: role of transforming growth factor-beta(1) and interferon-gamma
    Narayanan Venkatesan
    Meakins Christie Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada H2X 2P2
    Am J Physiol Lung Cell Mol Physiol 283:L806-14. 2002
    ..Activation of fibroblasts by TGF-beta(1) promotes abnormal deposition of PGs in fibrotic lungs; downregulation of TGF-beta(1) by IFN-gamma may have potential therapeutic benefits in this disease...
  46. ncbi request reprint Consortium for osteogenesis imperfecta mutations in the helical domain of type I collagen: regions rich in lethal mutations align with collagen binding sites for integrins and proteoglycans
    Joan C Marini
    Bone and Extracellular Matrix Branch, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Hum Mutat 28:209-21. 2007
    ..Our data on genotype-phenotype relationships indicate that the two collagen chains play very different roles in matrix integrity and that phenotype depends on intracellular and extracellular events...
  47. pmc Biglycan and fibromodulin fragmentation correlates with temporal and spatial annular remodelling in experimentally injured ovine intervertebral discs
    James Melrose
    Raymond Purves Laboratory, Institute of Bone and Joint Research, Kolling Institute of Medical Research, University of Sydney at Royal North Shore Hospital, St Leonards, 2065, NSW, Australia
    Eur Spine J 16:2193-205. 2007
    ....
  48. ncbi request reprint Measurement of glycosaminoglycan release from cartilage explants
    John S Mort
    Methods Mol Med 135:201-9. 2007
    ..Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method...
  49. pmc In vivo intervertebral disc remodeling: kinetics of mRNA expression in response to a single loading event
    Jeffrey J MaClean
    Spine Bioengineering Lab, College of Engineering and Mathematical Sciences, University of Vermont, 201 Perkins Hall, 33 Colchester Avenue, Burlington, Vermont 05405 0156, USA
    J Orthop Res 26:579-88. 2008
    ..Results have important implications for disc remodeling and tissue engineering...
  50. ncbi request reprint Reduced expression of the small leucine-rich proteoglycans, lumican, and decorin is associated with poor outcome in node-negative invasive breast cancer
    Sandra Troup
    Department of Pathology, University of Manitoba, Faculty of Medicine, Winnipeg, Manitoba, R3E OW3 Canada
    Clin Cancer Res 9:207-14. 2003
    ..To examine the prognostic significance of lumican and decorin, two abundant small leucine-rich proteoglycans in breast tissue stroma...
  51. doi request reprint Lumican inhibits collagen deposition in tissue engineered cartilage
    Wael Kafienah
    Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol, UK
    Matrix Biol 27:526-34. 2008
    ..These results suggest that lumican plays a pivotal role in the development of tissue engineered cartilage and that regulation of this protein may be important for the production of high-quality implants...
  52. ncbi request reprint The characterization of versican and its message in human articular cartilage and intervertebral disc
    Robert Sztrolovics
    Shriners Hospital for Children, Montreal, Que, Canada
    J Orthop Res 20:257-66. 2002
    ..The increased presence of versican in the disc relative to articular cartilage may suggest a more pronounced functional role for this proteoglycan, particularly in the nucleus pulposus...
  53. pmc Hyaluronate degradation as an alternative mechanism for proteoglycan release from cartilage during interleukin-1beta-stimulated catabolism
    Robert Sztrolovics
    Joint Diseases Laboratory, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada H3G 1A6
    Biochem J 362:473-9. 2002
    ..It is likely that both mechanisms contribute to cartilage metabolism in normal physiology and pathology...
  54. pmc The mechanism of aggrecan release from cartilage differs with tissue origin and the agent used to stimulate catabolism
    Robert Sztrolovics
    Joint Diseases Laboratory, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada H3G 1A6
    Biochem J 362:465-72. 2002
    ....
  55. ncbi request reprint Peptides of type II collagen can induce the cleavage of type II collagen and aggrecan in articular cartilage
    Tadashi Yasuda
    Shriners Hospitals for Children, Department of Surgery, McGill University, Montreal, Quebec, Canada H3G 1A6
    Matrix Biol 25:419-29. 2006
    ....
  56. ncbi request reprint SLRP interaction can protect collagen fibrils from cleavage by collagenases
    Yeqing Geng
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Matrix Biol 25:484-91. 2006
    ..Interaction with either recombinant decorin, fibromodulin or lumican results in decreased collagenase cleavage of both fibril types. Thus SLRP interaction can help protect collagen fibrils from cleavage by collagenases...
  57. ncbi request reprint Tumor necrosis factor-alpha modulates matrix production and catabolism in nucleus pulposus tissue
    Cheryle A Séguin
    BioEngineering of Skeletal Tissues Team, Mount Sinai Hospital, Ontario, Canada
    Spine (Phila Pa 1976) 30:1940-8. 2005
    ..This study examines changes in the production of extracellular matrix molecules as well as the induction of tissue degradation in in vitro formed nucleus pulposus (NP) tissues following incubation with tumor necrosis factor (TNF)alpha...
  58. pmc Analysis of intimal proteoglycans in atherosclerosis-prone and atherosclerosis-resistant human arteries by mass spectrometry
    Paul Talusan
    Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Mol Cell Proteomics 4:1350-7. 2005
    ..The enhanced deposition of lumican in the intima of an atherosclerosis prone artery has important implications for the pathogenesis of atherosclerosis...
  59. ncbi request reprint Insect cell conditioned medium contains an endoglycosidase able to liberate chondroitin sulfate chains from aggrecan
    Peter J Roughley
    Shriners Hospital for Children and Department of Surgery, McGill University, Montreal, Quebec, Canada
    Matrix Biol 24:371-5. 2005
    ..It may also represent an impediment to such research when baculovirus systems are used to generate recombinant proteoglycans...
  60. ncbi request reprint Link protein has greater affinity for versican than aggrecan
    Shuiliang Shi
    Joint Diseases Laboratory, Shriners Hospitals for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    J Biol Chem 279:12060-6. 2004
    ..These observations suggest that link protein may function as a stabilizer of the interaction, not only between aggrecan and hyaluronan in cartilage, but also between versican and hyaluronan in many tissues...
  61. ncbi request reprint Aggrecan turnover in human intervertebral disc as determined by the racemization of aspartic acid
    Sarit S Sivan
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 281:13009-14. 2006
    ....
  62. pmc Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis
    Allan A Young
    Raymond Purves Research Laboratory, Institute of Bone and Joint Research, Royal North Shore Hospital, University of Sydney, St Leonards, New South Wales, Australia
    Arthritis Res Ther 7:R852-61. 2005
    ..This study highlights the focal nature of the degenerative changes that occur in OA cartilage and suggests that altered synthesis and proteolysis of SLRPs may play an important role in cartilage destruction in arthritis...
  63. ncbi request reprint Production of antibodies against degradative neoepitopes in aggrecan
    John S Mort
    Joint Diseases Laboratory, Shriners Hospital for Children, and Departments of Surgery and Medicine, McGill University, Montreal, Quebec, Canada
    Methods Mol Med 100:237-50. 2004
    ..Limitations associated with the use of antipeptide antibodies to study authentic protein neoepitopes are discussed...
  64. pmc Effects of mechanical loading on intervertebral disc metabolism in vivo
    James C Iatridis
    University of Vermont, Burlington, VT 05405, USA
    J Bone Joint Surg Am 88:41-6. 2006
    ....
  65. ncbi request reprint The glycosaminoglycan attachment regions of human aggrecan
    Elida Rodriguez
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec H3G 1A6, Canada
    J Biol Chem 281:18444-50. 2006
    ..There was, however, no change in the sulfation pattern of the disaccharide repeats in the CS chains from the two domains...
  66. ncbi request reprint Genetic rescue of chondrodysplasia and the perinatal lethal effect of cartilage link protein deficiency
    Matyas Czipri
    Section of Biochemistry and Molecular Biology, Departments of Orthopedic Surgery and Biochemistry, Rush University at Rush Presbyterian St Luke s Medical Center, Chicago, Illinois 60612, USA
    J Biol Chem 278:39214-23. 2003
    ....
  67. ncbi request reprint The potential and limitations of a cell-seeded collagen/hyaluronan scaffold to engineer an intervertebral disc-like matrix
    Mauro Alini
    Orthopaedic Research Laboratory, Royal Victoria Hospital, McGill University, Montreal, Canada
    Spine (Phila Pa 1976) 28:446-54; discussion 453. 2003
    ..The use of a cell-seeded biomatrix for tissue engineering of the intervertebral disc...
  68. pmc Excess biglycan causes eyelid malformation by perturbing muscle development and TGF-alpha signaling
    Yasuhito Hayashi
    Department of Ophthalmology, University of Cincinnati Medical Center, Cincinnati, OH 45267 0527, United States
    Dev Biol 277:222-34. 2005
    ....
  69. ncbi request reprint Osteogenesis imperfecta type VI: a form of brittle bone disease with a mineralization defect
    Francis H Glorieux
    Shriners Hospital for Children, and Department of Surgery, McGill University, Montreal, Quebec, Canada
    J Bone Miner Res 17:30-8. 2002
    ..The underlying genetic defect remains to be elucidated...
  70. ncbi request reprint Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between Endoglin and Lumican expression
    Luisa M Botella
    Centro de Investigaciones Biologicas CSIC, Ramiro de Maeztu, 9 Madrid, Spain
    Matrix Biol 22:561-72. 2004
    ..This report describes for the first time a relationship between endoglin and lumican expression...
  71. doi request reprint Cartilage in normal and osteoarthritis conditions
    Johanne Martel-Pelletier
    Osteoarthritis Research Unit, University of Montreal Hospital Centre, Montreal, Canada
    Best Pract Res Clin Rheumatol 22:351-84. 2008
    ..The alterations in osteoarthritic cartilage are numerous and involve morphologic and metabolic changes in chondrocytes, as well as biochemical and structural alterations in the extracellular matrix macromolecules...
  72. ncbi request reprint Alterations in lung mechanics in decorin-deficient mice
    Anita Fust
    Meakins Christie Laboratories, Shriner s Hospital for Crippled Children, McGill University, Montreal, Quebec, Canada H2X 2P2
    Am J Physiol Lung Cell Mol Physiol 288:L159-66. 2005
    ..These alterations in lung tissue mechanical behavior in Dcn-/- mice support a critical role for decorin in the formation of the lung collagen network...
  73. ncbi request reprint Identification of beta1 integrin as mediator of melanoma cell adhesion to lumican
    Marie France D'Onofrio
    Laboratoire de Biochimie Médicale et Biologie Moléculaire, CNRS UMR 6198, Faculte de Medecine, IFR 53, Universite de Reims Champagne Ardenne, 51095 Reims Cedex, France
    Biochem Biophys Res Commun 365:266-72. 2008
    ..The increased beta1 integrin-mediated adhesion of melanoma cells to lumican might explain, at least in part, the anti-invasive effect of this SLRP...
  74. ncbi request reprint The use of biochemical markers of bone and cartilage metabolism to monitor osteoarthritis--dreams and reality
    Peter J Roughley
    J Rheumatol 30:910-2. 2003
  75. ncbi request reprint Lumican is a major proteoglycan component of the bone matrix
    Afshin Raouf
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Ont, Toronto, Canada
    Matrix Biol 21:361-7. 2002
    ....
  76. ncbi request reprint Cartilage-specific constitutive expression of TSG-6 protein (product of tumor necrosis factor alpha-stimulated gene 6) provides a chondroprotective, but not antiinflammatory, effect in antigen-induced arthritis
    Tibor T Glant
    Rush University, Rush Presbyterian St Luke s Medical Center, Chicago, Illinois 60612, USA
    Arthritis Rheum 46:2207-18. 2002
    ..To study the chondroprotective effect of constitutively expressed TSG-6 protein (tumor necrosis factor alpha-induced protein 6; Tnfip6) in cartilage, using antigen-induced arthritis (AIA) in mice...
  77. ncbi request reprint What is intervertebral disc degeneration, and what causes it?
    Michael A Adams
    Department of Anatomy, University of Bristol, Bristol, United Kingdom
    Spine (Phila Pa 1976) 31:2151-61. 2006
    ..Review and reinterpretation of existing literature...