Research Topics
| Benjamin RappazSummaryAffiliation: McGill University Country: Canada Publications
| Collaborators |
Detail Information
Publications
Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion mediumBenjamin Rappaz
Ecole Polytechnique Federale de Lausanne, Brain Mind Institute, Lausanne, Switzerland
Opt Lett 33:744-6. 2008..The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength...
Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopyBenjamin Rappaz
Ecole Polytechnique Federale de Lausanne, Brain Mind Institute, CH 1015 Lausanne, Switzerland
J Biomed Opt 14:034049. 2009..Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening...- Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflectionJonas Kuhn
Institute of Imaging and Applied Optics, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
Opt Lett 34:653-5. 2009..6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions... 
Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickeringDaniel Boss
Brain Mind Institute, Ecole Polytechnique Federale de Lausanne, Lausanne, Vaud, Switzerland
PLoS ONE 7:e40667. 2012..Further, our experiments show that the role of ATP as a driving force of CMF is questionable. ATP, however, seems to be required to maintain the unique biomechanical properties of the RBC membrane that lead to thermally excited CMF...- Living specimen tomography by digital holographic microscopy: morphometry of testate amoebaFlorian Charrière
Ecole Polytechnique Federale de Lausanne, Imaging and Applied Optics Institute, CH 1015 Lausanne, Switzerland
Opt Express 14:7005-13. 2006..Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%... - Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzerBenjamin Rappaz
Brain Mind Institute, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
Cytometry A 73:895-903. 2008..In conclusion, DHM in combination with a decoupling procedure allows measuring noninvasively volume, refractive index, and hemoglobin content of single-living RBCs with a high accuracy... - Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopyBenjamin Rappaz
Brain Mind Institute, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
Blood Cells Mol Dis 42:228-32. 2009..CMF amplitudes of 35.9+/-8.9 nm and 4.7+/-0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively... - Determination of transmembrane water fluxes in neurons elicited by glutamate ionotropic receptors and by the cotransporters KCC2 and NKCC1: a digital holographic microscopy studyPascal Jourdain
Brain and Mind Institute, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
J Neurosci 31:11846-54. 2011..This observation is of particular interest since it shows that DHM is the first imaging technique able to monitor dynamically and in situ the activity of these cotransporters during physiological and/or pathological neuronal conditions... - Simultaneous optical recording in multiple cells by digital holographic microscopy of chloride current associated to activation of the ligand-gated chloride channel GABA(A) receptorPascal Jourdain
Brain and Mind Institute, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
PLoS ONE 7:e51041. 2012..Finally, chloride currents can be determined and pharmacologically characterized non-invasively simultaneously on a large cellular sampling by DHM... - Digital holographic microscopy applied to life sciencesChristian Depeursinge
Institute of Applied Optics, Laboratoire d Optique Appliquée, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
Conf Proc IEEE Eng Med Biol Soc 2007:6244-7. 2007..DHM has the advantage to be non-invasive (no phototoxicity, no contrast agents) and allows a high throughput measurements...