Affiliation: McGill University
- Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservationM Ndao
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B 200 Antwerp, Belgium
Parasite 11:103-6. 2004..Cryopreservation of trypanosomes using glycerol yielded 90% viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35%...
- Comparison of blood smear, antigen detection, and nested-PCR methods for screening refugees from regions where malaria is endemic after a malaria outbreak in Quebec, CanadaMomar Ndao
National Reference Centre for Parasitology, McGill University Centre for Tropical Diseases, Montreal General Hospital, Room R3 137, Montreal, Quebec, Canada H3G 1A4
J Clin Microbiol 42:2694-700. 2004..Our data demonstrate that PCR is, by far, the most powerful tool for such surveillance...
- Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivaxP J de Almeida
Institute of Tropical Medicine, Belgium
Acta Trop 66:45-50. 1997..Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques...
- Extension of the prophylactic effect of isometamidium against trypanosome infections in cattle using a biodegradable copolymerS Geerts
Institute of Tropical Medicine, Antwerpen, Belgium
Acta Trop 73:49-58. 1999..Statistical analysis showed that the incidence of trypanosomiasis was significantly lower in the SRD treated than in the i.m. treated group...
- PCR primer evaluation for the detection of trypanosome DNA in naturally infected goatsP J Pereira de Almeida
Institute of Tropical Medicine, Antwerp, Belgium
Vet Parasitol 80:111-6. 1998..The ORPHON5J primers yielded no positive results. Analyses with the GOL primers of putatively negative samples, yielded aberrant band patterns whose diagnostic significance still remains to be determined...
- Performance of enzyme-linked immunosorbent assays for detection of antibodies against T. congolense and T. vivax in goatsVeerle Lejon
Parasitology Department, Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
Vet Parasitol 116:87-95. 2003..congolense or T. vivax infections. Acceptable inter-plate repeatability was observed. The implications of results and technical requirements for ongoing applied research are discussed...
- Malaria epidemics and surveillance systems in CanadaJ Dick MacLean
Montreal General Hospital, McGill University Centre for Tropical Diseases, Quebec, Canada
Emerg Infect Dis 10:1195-201. 2004..The Canadian experience is likely to be generalizable to other industrialized countries where malaria is a reportable disease within a passive surveillance system...
- Malaria "epidemic" in Quebec: diagnosis and response to imported malariaMomar Ndao
National Reference Centre for Parasitology, McGill University, Montreal, Que
CMAJ 172:46-50. 2005..Imported malaria is an increasing problem. The arrival of 224 African refugees presented the opportunity to investigate the diagnosis and management of imported malaria within the Quebec health care system...
- Development and comparison of enzyme immunoassays for diagnosis of Chagas' disease using fixed forms of Trypanosoma cruzi (Epimastigotes, Amastigotes, and Trypomastigotes) and assessment of antigen stability for the three assaysMariolga Berrizbietia
National Reference Centre for Parasitology, Montreal General Hospital, McGill University, Quebec, Canada
J Clin Microbiol 42:1766-9. 2004..3%), and trypomastigotes (99.3%). The fixed-trypomastigote assay was stable over 4 months at 4 degrees C and room temperature. These data suggest that a fixed-trypomastigote EIA may be a suitable candidate for blood bank screening...
- Purified excreted-secreted antigens from Trypanosoma cruzi trypomastigotes as tools for diagnosis of Chagas' diseaseMariolga Berrizbeitia
McGill Center for Tropical Diseases, Montreal General Hospital, Room D7 153, Montreal, Quebec, H3G IA4 Canada
J Clin Microbiol 44:291-6. 2006..A 60-kDa polypeptide was identified as a major contributor to the cross-reactivity with Leishmania. These data suggest the need for field validation studies of TESA- and TESA(IA)-based assays in regions where Chagas' disease is endemic...
- Development of a genetic assay to distinguish between Leishmania viannia species on the basis of isoenzyme differencesWen Wei Zhang
Department of Microbiology and Immunology, McGill University, Montreal, Canada
Clin Infect Dis 42:801-9. 2006..However, because of the close genetic similarity of L. braziliensis and L. peruviana, there currently exists no simple assay to distinguish between these species...