Van Luu-The

Summary

Country: Canada

Publications

  1. ncbi Type 5 17beta-hydroxysteroid dehydrogenase: its role in the formation of androgens in women
    V Luu-The
    Medical Research Council Group in Molecular Endocrinology, Oncology and Molecular Endocrinology Research Center, Laval University Hospital CHUL and Laval University, 2705 Laurier Boulevard, G1V 4G2, Quebec, Canada
    Mol Cell Endocrinol 171:77-82. 2001
  2. ncbi Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction
    Van Luu-The
    Molecular Endocrinology and Oncology Research Center, Laval University, Quebec, Canada
    Biotechniques 38:287-93. 2005
  3. ncbi Quantitative appreciation of steroidogenic gene expression in mouse tissues: new roles for type 2 5alpha-reductase, 20alpha-hydroxysteroid dehydrogenase and estrogen sulfotransferase
    Van Luu-The
    Molecular Endocrinology and Oncology Research Center, Laval University Hospital Research Center CRCHUL, Laval University, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 93:269-76. 2005
  4. ncbi Androgen biosynthetic pathways in the human prostate
    Van Luu-The
    Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center, Laval University, Quebec, Canada
    Best Pract Res Clin Endocrinol Metab 22:207-21. 2008
  5. ncbi Expression profiles of phases 1 and 2 metabolizing enzymes in human skin and the reconstructed skin models Episkin and full thickness model from Episkin
    Van Luu-The
    Oncology, Molecular Endocrinology and Genomic Research Center, Quebec University Hospital Research Center CRCHUQ and Laval University, 2705 Laurier Boulevard, Quebec G1 V 4G2, Canada
    J Steroid Biochem Mol Biol 116:178-86. 2009
  6. ncbi The intracrine sex steroid biosynthesis pathways
    Van Luu-The
    Oncology, Molecular Endocrinology and Human Genomics Research Center CREMOGH, Department of Molecular Medicine, Laval University and Laval University Hospital Research Center CRCHUL, Quebec, Canada
    Prog Brain Res 181:177-92. 2010
  7. ncbi Analysis and characteristics of multiple types of human 17beta-hydroxysteroid dehydrogenase
    V Luu-The
    Medical Research Council Group in Molecular Endocrinology, Oncology and Molecular Endocrinology Research Center, Laval University Hospital Center CHUL and Laval University, Quebec, G1V 4G2, Sainte Foy, Canada
    J Steroid Biochem Mol Biol 76:143-51. 2001
  8. ncbi Steroid metabolism and profile of steroidogenic gene expression in Episkin: high similarity with human epidermis
    Van Luu-The
    Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center CRCHUL and Laval University, 2705 Laurier Boulevard, Quebec G1V 4G2, Canada
    J Steroid Biochem Mol Biol 107:30-6. 2007
  9. ncbi Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids
    Jean Francois Couture
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL 2705 boul Laurier, Ste Foy, QC, Canada G1V 4G2
    J Mol Biol 331:593-604. 2003
  10. ncbi Tetrahydrogestrinone induces a genomic signature typical of a potent anabolic steroid
    Fernand Labrie
    Molecular Endocrinology and Oncology Laboratory, Laval University Medical Research Center CRCHUL and Laval University, Quebec City, G1V 4G2, Canada
    J Endocrinol 184:427-33. 2005

Collaborators

Detail Information

Publications73

  1. ncbi Type 5 17beta-hydroxysteroid dehydrogenase: its role in the formation of androgens in women
    V Luu-The
    Medical Research Council Group in Molecular Endocrinology, Oncology and Molecular Endocrinology Research Center, Laval University Hospital CHUL and Laval University, 2705 Laurier Boulevard, G1V 4G2, Quebec, Canada
    Mol Cell Endocrinol 171:77-82. 2001
    ..The double activity of type 5 17beta-HSD in the female reproductive tissues is probably necessary to the control of the optimal level of progesterone and sex steroids...
  2. ncbi Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction
    Van Luu-The
    Molecular Endocrinology and Oncology Research Center, Laval University, Quebec, Canada
    Biotechniques 38:287-93. 2005
    ..Using triplicate experiments, we show that measurement variations using our method are low with a mean of variation coefficients of <1%...
  3. ncbi Quantitative appreciation of steroidogenic gene expression in mouse tissues: new roles for type 2 5alpha-reductase, 20alpha-hydroxysteroid dehydrogenase and estrogen sulfotransferase
    Van Luu-The
    Molecular Endocrinology and Oncology Research Center, Laval University Hospital Research Center CRCHUL, Laval University, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 93:269-76. 2005
    ..On the other hand estrogen sulfotransferase, the enzyme that inactivates estrogen, has been found selectively expressed in male tissues, thus suggesting a role for this enzyme to protect male-specific tissues against estrogenic activity...
  4. ncbi Androgen biosynthetic pathways in the human prostate
    Van Luu-The
    Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center, Laval University, Quebec, Canada
    Best Pract Res Clin Endocrinol Metab 22:207-21. 2008
    ..In the present chapter, we review the data in order to provide a clearer picture of the pathways involved in DHT biosynthesis and metabolism in the human prostate...
  5. ncbi Expression profiles of phases 1 and 2 metabolizing enzymes in human skin and the reconstructed skin models Episkin and full thickness model from Episkin
    Van Luu-The
    Oncology, Molecular Endocrinology and Genomic Research Center, Quebec University Hospital Research Center CRCHUQ and Laval University, 2705 Laurier Boulevard, Quebec G1 V 4G2, Canada
    J Steroid Biochem Mol Biol 116:178-86. 2009
    ..FTM is a full thickness reconstructed skin model that also contains fibroblasts seeded in a collagen matrix...
  6. ncbi The intracrine sex steroid biosynthesis pathways
    Van Luu-The
    Oncology, Molecular Endocrinology and Human Genomics Research Center CREMOGH, Department of Molecular Medicine, Laval University and Laval University Hospital Research Center CRCHUL, Quebec, Canada
    Prog Brain Res 181:177-92. 2010
    ..We also examine the molecular evolution of steroidogenic enzymes which explains the major differences in steroid metabolism observed between laboratory animals and humans...
  7. ncbi Analysis and characteristics of multiple types of human 17beta-hydroxysteroid dehydrogenase
    V Luu-The
    Medical Research Council Group in Molecular Endocrinology, Oncology and Molecular Endocrinology Research Center, Laval University Hospital Center CHUL and Laval University, Quebec, G1V 4G2, Sainte Foy, Canada
    J Steroid Biochem Mol Biol 76:143-51. 2001
    ..It is noteworthy that rat type 6 17beta-HSD also catalyzes the reaction in the oxidative direction. In this report, we analyze the different characteristics of the multiple types of human 17beta-HSD...
  8. ncbi Steroid metabolism and profile of steroidogenic gene expression in Episkin: high similarity with human epidermis
    Van Luu-The
    Laboratory of Molecular Endocrinology and Oncology, Laval University Hospital Research Center CRCHUL and Laval University, 2705 Laurier Boulevard, Quebec G1V 4G2, Canada
    J Steroid Biochem Mol Biol 107:30-6. 2007
    ..Except for UGT-glucuronosyl transferase, similar mRNA expression profiles between Episkin and human epidermis are observed...
  9. ncbi Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids
    Jean Francois Couture
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL 2705 boul Laurier, Ste Foy, QC, Canada G1V 4G2
    J Mol Biol 331:593-604. 2003
    ..We thus propose that Prog is maintained in a new steroid-binding site composed mainly of residues found in the carboxy-terminal region of the protein...
  10. ncbi Tetrahydrogestrinone induces a genomic signature typical of a potent anabolic steroid
    Fernand Labrie
    Molecular Endocrinology and Oncology Laboratory, Laval University Medical Research Center CRCHUL and Laval University, Quebec City, G1V 4G2, Canada
    J Endocrinol 184:427-33. 2005
    ..The present microarray data provide an extremely precise and unquestionable signature of the androgenic/anabolic activity of THG, an approach which should apply to the analysis of the activity of any anabolic steroid...
  11. ncbi Loop relaxation, a mechanism that explains the reduced specificity of rabbit 20alpha-hydroxysteroid dehydrogenase, a member of the aldo-keto reductase superfamily
    Jean Francois Couture
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Ste Foy, Quebec, Canada G1V 4G2
    J Mol Biol 339:89-102. 2004
    ....
  12. ncbi Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Dept of Nutrition, Laval University Medical Center, 2705 Laurier Boulevard, Rm T3 67, Quebec City, Prov Quebec, Canada G1V 4G2
    Am J Physiol Endocrinol Metab 288:E398-404. 2005
    ..Increased progesterone and/or dihydrotestosterone reduction in abdominal adipose tissue may impact locally on fat cell metabolism...
  13. ncbi Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Quebec QC, Canada G1V 4G2
    J Mol Biol 364:747-63. 2006
    ....
  14. ncbi Differential androgen and estrogen substrates specificity in the mouse and primates type 12 17beta-hydroxysteroid dehydrogenase
    Pierre Gilles Blanchard
    Oncology and Molecular Endocrinology Research Center, Laval University Hospital Research Center CRCHUL and Laval University, 2705 Laurier Boulevard, Quebec, Canada
    J Endocrinol 194:449-55. 2007
    ....
  15. ncbi Local androgen inactivation in abdominal visceral adipose tissue
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Quebec, Canada G1V 4G2
    J Clin Endocrinol Metab 88:5944-50. 2003
    ..Increased androgen inactivation in omental adipose tissue of abdominally obese women may impact locally on the regulation of adipocyte metabolism...
  16. ncbi Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, Laval University, Quebec, Canada G1V 4G2
    J Mol Biol 369:525-40. 2007
    ....
  17. ncbi Glucocorticoid-induced androgen inactivation by aldo-keto reductase 1C2 promotes adipogenesis in human preadipocytes
    Alain Veilleux
    Endocrinology and Genomics and Dept of Food Science and Nutrition, Laval University Medical Research Center, Laval University, Quebec City, QC, Canada
    Am J Physiol Endocrinol Metab 302:E941-9. 2012
    ..The interplay between glucocorticoids and AKR1C2-dependent androgen inactivation may locally modulate adipogenesis and lipid accumulation in a depot-specific manner...
  18. ncbi Isolation and characterization of a cDNA encoding mouse 3alpha-hydroxysteroid dehydrogenase: an androgen-inactivating enzyme selectively expressed in female tissues
    Véronique Bellemare
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, 2705 Laurier Boulevard, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 98:18-24. 2006
    ..This role could be related to the inactivation of excess of androgen and progesterone that are more severely regulated than in man...
  19. ncbi Crystal structures of human Delta4-3-ketosteroid 5beta-reductase (AKR1D1) reveal the presence of an alternative binding site responsible for substrate inhibition
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Laval, Quebec QC G1V 4G2, Canada
    Biochemistry 47:13537-46. 2008
    ..The results of this report provide structural insights into the substrate inhibition of h5beta-red by C19- and C21-steroids...
  20. ncbi Effects of androgens on adipocyte differentiation and adipose tissue explant metabolism in men and women
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Laval University, Quebec, QC, Canada
    Clin Endocrinol (Oxf) 72:176-88. 2010
    ..To examine the effects of aromatizable or nonaromatizable androgens on abdominal subcutaneous (SC) and omental (OM) adipose tissue lipid metabolism and adipogenesis in men and women...
  21. ncbi Response of the adipose tissue transcriptome to dihydrotestosterone in mice
    Yonghua Zhang
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Quebec, Canada
    Physiol Genomics 35:254-61. 2008
    ....
  22. ncbi Caenorhabditis elegans LET-767 is able to metabolize androgens and estrogens and likely shares common ancestor with human types 3 and 12 17beta-hydroxysteroid dehydrogenases
    Serge Desnoyers
    Pediatrics Research Unit, Laval University Medical Center CHUL and Laval University, 2705 Laurier Boulevard, Quebec, G1V 4G2 Canada
    J Endocrinol 195:271-9. 2007
    ..The high conservation of the active site and amino acids responsible for enzymatic activity and substrate selectivity strongly suggests that LET-767 shares a common ancestor with human 17beta-HSD3 and 12...
  23. ncbi Androgen inactivation and steroid-converting enzyme expression in abdominal adipose tissue in men
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Laval University, 2705 Laurier Boulevard (T3-67, , , Canada G1V 4G2
    J Endocrinol 191:637-49. 2006
    ..Further studies are required to elucidate whether local androgen inactivation in abdominal adipose tissue is involved in the modulation of adipocyte metabolism and regional fat distribution in men...
  24. ncbi Dehydroepiandrosterone (DHEA) is an anabolic steroid like dihydrotestosterone (DHT), the most potent natural androgen, and tetrahydrogestrinone (THG)
    Fernand Labrie
    Molecular Endocrinology and Oncology Laboratory, Laval University Hospital Research Center CRCHUL and Laval University, Quebec City, Canada G1V 4G2
    J Steroid Biochem Mol Biol 100:52-8. 2006
    ..The present microarray approach to identify anabolic compounds is applicable to all potential androgenic/anabolic compounds...
  25. ncbi Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone
    Fernand Labrie
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Center Centre Hospitalier de l Université Laval and Laval University, Quebec City, Quebec G1V 4G2, Canada
    Endocr Rev 24:152-82. 2003
    ....
  26. ncbi Specific transcriptional response of four blockers of estrogen receptors on estradiol-modulated genes in the mouse mammary gland
    Ezequiel Calvo
    Molecular Endocrinology, Oncology and Human Genomics Research Center, Laval University and Laval University Hospital Research Center, 2705 Laurier Blvd, Quebec, QC, G1V 4G2, Canada
    Breast Cancer Res Treat 134:625-47. 2012
    ..When used alone, ACOL modulated the smallest number of genes also influenced by E(2), namely 4 %, thus possibly explaining potential utilities of this compound in breast cancer prevention and therapy...
  27. ncbi Expression and localization of estrogenic type 12 17beta-hydroxysteroid dehydrogenase in the cynomolgus monkey
    Hong Liu
    Oncology and Molecular Endocrinology Laboratory, Laval University Hospital Research Center CRCHUL and Laval University, Quebec, GlV 4G2, Canada
    BMC Biochem 8:2. 2007
    ....
  28. ncbi Cloning and characterization of human form 2 type 7 17beta-hydroxysteroid dehydrogenase, a primarily 3beta-keto reductase and estrogen activating and androgen inactivating enzyme
    Hong Liu
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center and Laval University, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 94:173-9. 2005
    ..2. This newly identified form of 17beta-HSD7 could have a significant role by modulating active hormone levels in estrogen-sensitive cells or tissues...
  29. ncbi Expression of enzymes involved in synthesis and metabolism of estradiol in human breast as studied by immunocytochemistry and in situ hybridization
    Zhuo Li
    Oncology and Molecular Endocrinology Research Center, Laval University Hospital Research Centre CRCHUL and Laval University, Quebec City, Quebec, Canada
    Histol Histopathol 24:273-82. 2009
    ..The present results indicate the enzymes which play a role in the synthesis and metabolism of E2 are expressed in both epithelial and stromal cells in human breast...
  30. ncbi C6-(N,N-butyl-methyl-heptanamide) derivatives of estrone and estradiol as inhibitors of type 1 17beta-hydroxysteroid dehydrogenase: Chemical synthesis and biological evaluation
    Christine Cadot
    Oncology and Molecular Endocrinology Research Center, CHUQ Pavillon CHUL and Université Laval, Quebec, Canada
    Bioorg Med Chem 15:714-26. 2007
    ..Interestingly, the new inhibitors were also found less estrogenic than the lead compound in two breast cancer cell lines, T-47D and MCF-7...
  31. ncbi Inhibitors of type 1 17beta-hydroxysteroid dehydrogenase with reduced estrogenic activity: modifications of the positions 3 and 6 of estradiol
    Martin R Tremblay
    Oncology and Molecular Endocrinology Research Center, , CHUQ-Pavillon CHUL, 2705 Boulevard Laurier, , QC G1V 4G2, Canada
    J Enzyme Inhib Med Chem 20:153-63. 2005
    ..This study contributes to the knowledge required for the development of compounds with the desired profile, that is, a potent inhibitor of type 1 17beta-HSD without estrogen-like effects...
  32. ncbi Specific estradiol biosynthetic pathway in choriocarcinoma (JEG-3) cell line
    Melanie Samson
    Oncology and Molecular Endocrinology Research Center, CHUL Research Center, CHUQ, and Laval University Medical Center, 2705 Laurier Boulevard, Quebec, QC, Canada
    J Steroid Biochem Mol Biol 116:154-9. 2009
    ..Our data are clearly in favor of the pathway in which the step of aromatization precedes the step of reduction by 17beta-HSD...
  33. ncbi Structure-based inhibitor design for an enzyme that binds different steroids: a potent inhibitor for human type 5 17beta-hydroxysteroid dehydrogenase
    Wei Qiu
    Canadian Institutes of Health Research Group in Molecular Endocrinology, Laval University Medical Center, Centre Hospitalier de Universités de Québec and Laval University, Quebec G1V 4G2, Canada
    J Biol Chem 282:8368-79. 2007
    ....
  34. ncbi Assessment of the ability of type 2 cytochrome b5 to modulate 17,20-lyase activity of human P450c17
    Penny Soucy
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Ste Foy Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 80:71-5. 2002
    ..Furthermore, the analysis of mRNA expression of types 1 and 2 cyt-b5 by RT-PCR using primers specific to each type showed that both types are present in the liver but also in the adrenal and testis...
  35. ncbi 3Beta-sulfamate derivatives of C19 and C21 steroids bearing a t-butylbenzyl or a benzyl group: synthesis and evaluation as non-estrogenic and non-androgenic steroid sulfatase inhibitors
    Liviu C Ciobanu
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, Centre Hospitalier Universitaire de Quebec (CHUQ-Pavillon CHUL) 2705 Boulevard Laurier and Laval University, , QC, G1V 4G2, Canada
    J Enzyme Inhib Med Chem 18:15-26. 2003
    ....
  36. ncbi Potent and selective steroidal inhibitors of 17beta-hydroxysteroid dehydrogenase type 7, an enzyme that catalyzes the reduction of the key hormones estrone and dihydrotestosterone
    Edith Bellavance
    Laboratory of Medicinal Chemistry, Oncology and Molecular Endocrinology, CHUQ CHUL Research Center and Laval University, 2705 Boulevard Laurier, Quebec G1V 4G2, Canada
    J Med Chem 52:7488-502. 2009
    ..First selective and efficient inhibitors of 17beta-HSD7 are now available for additional mechanistic and therapeutic studies...
  37. ncbi [From the gene to the clinic: prostate cancer death can now be an exception?]
    Fernand Labrie
    Centre de recherche en endocrinologie moléculaire et oncologique, Centre de Recherche du CHUL, Centre Hospitalier de l Université Laval et Université Laval, Sainte Foy, Quebec, G1V 4G2 Canada
    Med Sci (Paris) 19:910-9. 2003
    ..Today's data show that with the simple application of the available diagnostic and therapeutic tools, death from prostate cancer should be an exception...
  38. ncbi Progesterone metabolism in adipose cells
    Yonghua Zhang
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Center, Quebec City, Canada
    Mol Cell Endocrinol 298:76-83. 2009
    ..In conclusion, preadipocytes and lipid-storing, mature adipocytes efficiently generate progesterone metabolites in women, which is consistent with rather modest effects progesterone on abdominal fat cell differentiation...
  39. ncbi Localization of type 8 17beta-hydroxysteroid dehydrogenase mRNA in mouse tissues as studied by in situ hybridization
    Georges Pelletier
    Oncology and Molecular Endocrinology Research Center, Laval University Hospital CHUL, Quebec, Canada
    J Histochem Cytochem 53:1257-71. 2005
    ..In the lung, type 8 17beta-HSD mRNA was detected in bronchial epithelial cells and walls of pulmonary arteries. The present data suggest that type 8 17beta-HSD can exert its action to downregulate E2 levels in a large variety of tissues...
  40. ncbi Characterization of type 12 17beta-hydroxysteroid dehydrogenase, an isoform of type 3 17beta-hydroxysteroid dehydrogenase responsible for estradiol formation in women
    Van Luu-The
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center, Quebec, Canada G1V 4G2
    Mol Endocrinol 20:437-43. 2006
    ..Overall, our results strongly suggest that 17beta-HSD12 is the major estrogenic 17beta-HSD responsible for the conversion of E1 to E2 in women, especially in the ovary, the predominant source of estrogens before menopause...
  41. ncbi Steroidal lactones as inhibitors of 17beta-hydroxysteroid dehydrogenase type 5: chemical synthesis, enzyme inhibitory activity, and assessment of estrogenic and androgenic activities
    Patrick Bydal
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, CHUL Research Center and University Laval, 2705 Laurier Boulevard, Quebec, Quebec G1V 4G2, Canada
    Eur J Med Chem 44:632-44. 2009
    ..03 microM. Interestingly, no significant proliferative effect was detected on Shionogi (AR+) cells in culture in the presence of 0.1 and 1 microM of lactone 26...
  42. ncbi Expression of 11β-hydroxysteroid dehydrogenase type 1 in breast cancer and adjacent non-malignant tissue. An immunocytochemical study
    Lu Lu
    Laval University Hospital Research Center, Quebec, QC, Canada
    Pathol Oncol Res 17:627-32. 2011
    ....
  43. ncbi Major impact of hormonal therapy in localized prostate cancer--death can already be an exception
    Fernand Labrie
    Oncology and Molecular Endocrinology Research Center, Le Centre Hospitalier de l Université Laval CHUL and Laval University, 2705 Laurier Boulevard, Quebec City, Quebec, Canada G1V 4G2
    J Steroid Biochem Mol Biol 92:327-44. 2004
    ..Most importantly, recent data show that the addition of a pure antiandrogen to an LHRH agonist in order to block the androgens made locally in the prostate leads to a 90% long-term control or probable cure of prostate cancer...
  44. ncbi Temporal analysis of E2 transcriptional induction of PTP and MKP and downregulation of IGF-I pathway key components in the mouse uterus
    Mahine Ivanga
    Oncology and Molecular Endocrinology Research Center, Centre Hospitalier de l Universite Laval Research Center, Centre Hospitalier Universitaire de Quebec, Department of Anatomy and Physiology, Laval University, Quebec, Canada
    Physiol Genomics 29:13-23. 2007
    ..Furthermore, the protein tyrosine phosphatases and MAPK phosphatases are likely to modulate the estrogenic uterotrophic action by targeting, at different steps, the IGF-I pathway...
  45. ncbi Differential estrogenic 17beta-hydroxysteroid dehydrogenase activity and type 12 17beta-hydroxysteroid dehydrogenase expression levels in preadipocytes and differentiated adipocytes
    Véronique Bellemare
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center and Laval University, Quebec, Canada
    J Steroid Biochem Mol Biol 114:129-34. 2009
    ..0001) in differentiated adipocytes. The increased estrogenic 17beta-HSD activity is consistent with the increase in protein expression levels of 17beta-HSD12...
  46. ncbi Molecular characterization of the cynomolgus monkey Macaca fascicularis steroidogenic enzymes belonging to the aldo-keto reductase family
    Hong Liu
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CRCHUL and Laval University, 2705 Laurier Boulevard, Quebec G1V 4G2, Canada
    J Steroid Biochem Mol Biol 104:75-80. 2007
    ..Monkey 20alpha-HSD mRNA is highly expressed in the kidney, stomach, and liver. Our study provides the basis for future investigations on the regulation and function of these enzymes in the monkey...
  47. ncbi Androgen metabolism in adipose tissue: recent advances
    Karine Blouin
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Canada Department of Nutrition, Laval University, Canada
    Mol Cell Endocrinol 301:97-103. 2009
    ..We hypothesize that body fat distribution patterns likely emerge from the local adipose tissue balance between active androgens and glucocorticoids in each fat compartment...
  48. ncbi Characterization of 17alpha-hydroxysteroid dehydrogenase activity (17alpha-HSD) and its involvement in the biosynthesis of epitestosterone
    Véronique Bellemare
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, 2705 Laurier Boulevard, Quebec, G1V 4G2, Canada
    BMC Biochem 6:12. 2005
    ..It was found to possess antiandrogenic activity as well as neuroprotective effects. So far, the exact pathway leading to the formation of epiT has not been elucidated...
  49. ncbi 3Beta-alkyl-androsterones as inhibitors of type 3 17beta-hydroxysteroid dehydrogenase: inhibitory potency in intact cells, selectivity towards isoforms 1, 2, 5 and 7, binding affinity for steroid receptors, and proliferative/antiproliferative activities o
    Béatrice Tchédam Ngatcha
    Oncology and Molecular Endocrinology Research Center, CHUL Research Center and Université Laval, CHUQ Pavillon CHUL, 2705 Boulevard Laurier, Que, Canada G1V 4G2
    Mol Cell Endocrinol 248:225-32. 2006
    ..The more potent inhibitors were 3beta-hexyl-ADT (2), 3beta-cyclohexylethyl-ADT (4) and 3beta-phenylethyl-ADT (7)...
  50. ncbi Androsterone 3alpha-ether-3beta-substituted and androsterone 3beta-substituted derivatives as inhibitors of type 3 17beta-hydroxysteroid dehydrogenase: chemical synthesis and structure-activity relationship
    Béatrice Tchédam Ngatcha
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, CHUQ Pavillon CHUL and Université Laval, 2705 Boulevard Laurier, Quebec G1V 4G2, Canada
    J Med Chem 48:5257-68. 2005
    ..The results of our study identified potent type 3 17beta-HSD inhibitors for potential use in the treatment of androgen-sensitive diseases...
  51. ncbi Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor. Role of recombinant cytochrome b5 and P450 reductase
    Penny Soucy
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Center CHUL and Laval University, Quebec, Canada
    Eur J Biochem 270:1349-55. 2003
    ..Our results therefore demonstrate that human P450c17, as other enzymes of the classical steroidogenic pathway, is involved in the biosynthetic pathway leading to the formation of androstenol...
  52. ncbi Inhibition of estrone sulfate-induced uterine growth by potent nonestrogenic steroidal inhibitors of steroid sulfatase
    Liviu C Ciobanu
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, , 2705 Laurier Boulevard, , Canada G1V 4G2
    Cancer Res 63:6442-6. 2003
    ..This report clearly shows that a steroid sulfatase inhibitor can efficiently block estrogen action from the inactive precursor estrone sulfate, in vitro and in vivo...
  53. ncbi Expression of enzymes involved in estrogen metabolism in human prostate
    Yasukazu Takase
    Oncology and Molecular Endocrinology Laboratory, CHUL Research Center, 2705 Laurier Boulevard, , G1V 4G2, Canada
    J Histochem Cytochem 54:911-21. 2006
    ....
  54. ncbi Comparison of crystal structures of human type 3 3alpha-hydroxysteroid dehydrogenase reveals an "induced-fit" mechanism and a conserved basic motif involved in the binding of androgen
    Jean Francois Couture
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center and Laval University, Quebec, Canada
    Protein Sci 14:1485-97. 2005
    ..All these results have thus been used to reevaluate the binding mode of this enzyme for androgens...
  55. ncbi Visceral adiposity and endothelial lipase
    Marie Eve Paradis
    Nutraceuticals and Functional Foods Institute, 2440 Boulevard Hochelaga, Laval University, Quebec, Canada
    J Clin Endocrinol Metab 91:3538-43. 2006
    ..However, the extent to which EL contributes to modulate the deteriorated high-density lipoprotein profile observed in obesity in humans is less clear...
  56. ncbi Gonadotropin-releasing hormone agonists in the treatment of prostate cancer
    Fernand Labrie
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center Centre Hospitalier de l Université Laval, 2705 Laurier Boulevard, Quebec City, Quebec, Canada
    Endocr Rev 26:361-79. 2005
    ....
  57. ncbi Expression and activity of 20alpha-hydroxysteroid dehydrogenase (AKR1C1) in abdominal subcutaneous and omental adipose tissue in women
    Sophie Blanchette
    Molecular Endocrinology and Oncology Research Center, Laval University Medical Center, 2705 Laurier Boulevard, Room T3-67, , PQ, Canada G1V 4G2
    J Clin Endocrinol Metab 90:264-70. 2005
    ..Additional studies are required to establish whether local conversion of progesterone may impact on the metabolism and function of adipocytes located within the abdominal cavity...
  58. ncbi Omental adipose tissue type 1 11 beta-hydroxysteroid dehydrogenase oxoreductase activity, body fat distribution, and metabolic alterations in women
    Alain Veilleux
    Department of Food Science and Nutrition, Laval University, Quebec, Canada
    J Clin Endocrinol Metab 94:3550-7. 2009
    ....
  59. ncbi Nonsteroidal compounds designed to mimic potent steroid sulfatase inhibitors
    Liviu Constantin Ciobanu
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, , Pavillon CHUL T3-67, 2705 Laurier Boulevard, Sainte-Foy, Qu, Canada G1V 4G2
    J Steroid Biochem Mol Biol 80:339-53. 2002
    ..Such results suggest that compound from this later category better mimic the steroidal inhibitor...
  60. ncbi Expression, crystallization and preliminary X-ray analysis of human and rabbit 20alpha-hydroxysteroid dehydrogenases in complex with NADP(H) and various steroid substrates
    Jean Francois Couture
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Quebec G1V 4G2, Canada
    Acta Crystallogr D Biol Crystallogr 58:135-9. 2002
    ..7 A) or 4-androstenedione (1.8 A). All these crystals belong to the monoclinic space group P2(1). A partial data set has also been collected for a crystal of h20alpha-HSD (P2(1)2(1)2(1)) in complex with NADP(H) and progesterone...
  61. ncbi Inhibition of human-type 1 3beta-hydroxysteroid deshydrogenase/Delta(5)-Delta(4)-isomerase expression using siRNA
    Melanie Samson
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center, 2705 Laurier Boulevard, Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 94:253-7. 2005
    ..Up to 98% inhibition has been observed. To our knowledge, this is the first report showing successful inhibition of steroidogenic enzymes using siRNA technology...
  62. ncbi Human type 1 estrogen sulfotransferase: catecholestrogen metabolism and potential involvement in cancer promotion
    Frederic Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center (CHUL) and Laval University, Quebec G1V 4G2, Canada
    Ann N Y Acad Sci 963:221-8. 2002
    ..Indeed, hEST1 may also be involved in the production of stable precursors for local steroid biosynthesis or in the activation of promutagenic estrogen metabolites into carcinogens...
  63. ncbi Synthesis and optimization of a new family of type 3 17 beta-hydroxysteroid dehydrogenase inhibitors by parallel liquid-phase chemistry
    René Maltais
    Medicinal Chemistry Division, Oncology and Molecular Endocrinology Research Center, Centre Hospitalier Universitaire de Québec and Université Laval, 2705 Laurier Boulevard, Sainte Foy, Quebec G1V 4G2, Canada
    J Med Chem 45:640-53. 2002
    ....
  64. ncbi Analysis of 17beta-hydroxysteroid dehydrogenase types 5, 7, and 12 genetic sequence variants in breast cancer cases from French Canadian Families with high risk of breast and ovarian cancer
    Marie Plourde
    Cancer Genomics Laboratory, Endocrinology and Genomics Division CHUQ Research Center and Laval University, Quebec, Canada
    J Steroid Biochem Mol Biol 116:134-53. 2009
    ..Finally, a haplotype-based approach was used to determine tagging SNPs providing valuable information for studies investigating associations of common variants in these genes with breast cancer risk...
  65. ncbi Structure-activity relationships of a series of analogs of the endozepine octadecaneuropeptide (ODN(11)(-)(18)) on neurosteroid biosynthesis by hypothalamic explants
    Jean Luc Do Rego
    INSERM U413, Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research IFRMP 23, University of Rouen, 76821 Mont Saint Aignan, France
    J Med Chem 50:3070-6. 2007
    ..The present study indicates that the C-terminal hexapeptide of ODN/OP is the minimal sequence retaining full biological activity on steroid-producing neurons...
  66. ncbi Neuropeptide Y inhibits the biosynthesis of sulfated neurosteroids in the hypothalamus through activation of Y(1) receptors
    Delphine Beaujean
    European Institute for Peptide Research (IFRMP 23, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U-413, UA Centre National de la Recherche Scientifique, University of Rouen, 76821 Mont-Saint-Aignan, France
    Endocrinology 143:1950-63. 2002
    ..Our data also demonstrate that NPY, acting via Y(1) receptors, exerts an inhibitory effect on the biosynthesis of sulfated neurosteroids...
  67. ncbi Vasotocin and mesotocin stimulate the biosynthesis of neurosteroids in the frog brain
    Jean-Luc Do-Rego
    , , Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research, University of Rouen, 76821 Mont-Saint-Aignan, France
    J Neurosci 26:6749-60. 2006
    ..The present study provides the first evidence for a regulatory effect of VT and MT on neurosteroid biosynthesis. These data suggest that neurosteroids may mediate some of the behavioral actions of VT and MT...
  68. ncbi Androsterone derivatives substituted at position 16: chemical synthesis, inhibition of type 3 17beta-hydroxysteroid dehydrogenase, binding affinity for steroid receptors and proliferative/antiproliferative activity on Shionogi (AR+) cells
    Béatrice Tchédam-Ngatcha
    J Enzyme Inhib Med Chem 17:155-65. 2002
    ....
  69. ncbi The crystal structure of human Delta4-3-ketosteroid 5beta-reductase defines the functional role of the residues of the catalytic tetrad in the steroid double bond reduction mechanism
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center, Quebec QC, G1V 4G2 Canada
    Biochemistry 47:8261-70. 2008
    ....
  70. ncbi Immunohistochemical localization and biological activity of the steroidogenic enzyme cytochrome P450 17alpha-hydroxylase/C17, 20-lyase (P450C17) in the frog brain and pituitary
    Jean Luc Do Rego
    INSERM U413, Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research IFRMP 23, University of Rouen, Mont Saint Aignan, France
    J Neurochem 100:251-68. 2007
    ..This is the first detailed immunohistochemical mapping of P450(C17) in the brain and pituitary of any vertebrate. Altogether, the present data provide evidence that CNS neurons and pituitary cells can synthesize androgens...
  71. ncbi Ontogeny of 3 beta-hydroxysteroid dehydrogenase and 5 alpha-reductase in the frog brain
    Mauro Vallarino
    Department of Experimental Biology, University of Genova, Italy
    Ann N Y Acad Sci 1040:490-3. 2005
    ..These observations indicate that biosynthesis of biologically active steroids occurs in the brain of tadpoles, suggesting that neurosteroids may play a role in brain development...
  72. ncbi Microarray analysis of survival pathways in human PC-3 prostate cancer cells
    R Tenta
    Department of Experimental Physiology, Medical School, University of Athens, Greece
    Cancer Genomics Proteomics 4:309-18. 2007
    ..Insulin-like growth factor 1 (IGF-1), transforming growth factor beta 1 (TGFbeta1), and interleukin 6 (IL-6), act as survival factors inhibiting chemotherapy-induced apoptosis in PC-3 human prostate cancer cells, in vitro...
  73. ncbi Isolation and characterization of goat ovarian aromatase cDNA: assessment of the activity using an intact cell system and placental expression
    Raúl José Bobes
    Dpto de Fisiologia, Biofisica y NC, CINVESTAV IPN, Apdo Postal 14 740, 07000 Mexico D F, Mexico
    Theriogenology 62:532-43. 2004
    ....